Pengaruh Ekstrak Metanol Daun Suren (Toona sureni Bl Merr) Terhadap Fungsi Hati dan Nilai Darah (Rattus norvegicus L.) Jantan yang Dipapari Karbon Tetraklorida (CCl4)
Lampiran 1. Hasil Uji Skrining Fitokimia Ekstrak Metanol Daun Suren
Universitas Sumatera Utara
Lampiran 2. Data Pengamatan Rata-Rata Kadar SGPT, SGOT, Eritrosit
dan Kadar HbTikus putih yang diberi ekstrak metanol suren
dengan dosis 100 mg/kg BB, 150 mg/kg BB,dan 200 mg/kg/BB
Kelompok
Rata-rata Kadar
SGPT(µ/L)
Rata-rata Kadar
SGOT (µ/L)
Rata-rata
Jumlah Eritrosit
(x 106/mm3)
Rata-rata
Kadar Hb
(g/100mL)
C10H
C24H
MK10H
MK24H
K
CS100
CS150
CS200
255,35
359,9
242,36
211,62
139,87
198,1
287,25
228,25
371,47
362,75
343,22
354,97
315,02
272,25
245,5
236,7
7,42
7,31
7,61
5,97
7,07
9,74
6,75
8,86
15,42
12,42
13,85
13,1
11,65
12,47
15,65
13,05
Universitas Sumatera Utara
Lampiran 3. Hasil Analisis SPSS 20 untuk Kadar SGPT
ANOVA
Kadar_SGPT
Sum of Squares
Df
Mean Square
F
Sig.
Between Groups
118290,701
7
16898,672
4,040
,005
Within Groups
100399,936
24
4183,331
Total
218690,638
31
Multiple Comparisons
Dependent Variable: Kadar_SGPT
Bonferroni
(I)
(J) Perlakuan
Perlakuan
C24H
C10H
C24H
MK10H
MK24H
Mean
Std. Error
Sig.
95% Confidence Interval
Difference (I-
Lower
J)
Bound
Upper Bound
-104,55000
45,73473
,879
-265,1988
56,0988
MK10H
12,99000
45,73473
1,000
-147,6588
173,6388
MK24H
43,72500
45,73473
1,000
-116,9238
204,3738
K
115,47500
45,73473
,521
-45,1738
276,1238
CS100
57,25000
45,73473
1,000
-103,3988
217,8988
CS150
-31,90000
45,73473
1,000
-192,5488
128,7488
CS200
27,10000
45,73473
1,000
-133,5488
187,7488
C10H
104,55000
45,73473
,879
-56,0988
265,1988
MK10H
117,54000
45,73473
,470
-43,1088
278,1888
MK24H
148,27500
45,73473
,097
-12,3738
308,9238
220,02500
*
45,73473
,002
59,3762
380,6738
CS100
161,80000
*
45,73473
,047
1,1512
322,4488
CS150
72,65000
45,73473
1,000
-87,9988
233,2988
CS200
131,65000
45,73473
,231
-28,9988
292,2988
C10H
-12,99000
45,73473
1,000
-173,6388
147,6588
C24H
-117,54000
45,73473
,470
-278,1888
43,1088
MK24H
30,73500
45,73473
1,000
-129,9138
191,3838
K
102,48500
45,73473
,968
-58,1638
263,1338
CS100
44,26000
45,73473
1,000
-116,3888
204,9088
CS150
-44,89000
45,73473
1,000
-205,5388
115,7588
CS200
14,11000
45,73473
1,000
-146,5388
174,7588
C10H
-43,72500
45,73473
1,000
-204,3738
116,9238
C24H
-148,27500
45,73473
,097
-308,9238
12,3738
MK10H
-30,73500
45,73473
1,000
-191,3838
129,9138
K
71,75000
45,73473
1,000
-88,8988
232,3988
CS100
13,52500
45,73473
1,000
-147,1238
174,1738
K
Universitas Sumatera Utara
K
CS150
-75,62500
45,73473
1,000
-236,2738
85,0238
CS200
-16,62500
45,73473
1,000
-177,2738
144,0238
C10H
-115,47500
45,73473
,521
-276,1238
45,1738
C24H
-220,02500
*
45,73473
,002
-380,6738
-59,3762
MK10H
-102,48500
45,73473
,968
-263,1338
58,1638
MK24H
-71,75000
45,73473
1,000
-232,3988
88,8988
CS100
-58,22500
45,73473
1,000
-218,8738
102,4238
CS150
-147,37500
45,73473
,102
-308,0238
13,2738
CS200
-88,37500
45,73473
1,000
-249,0238
72,2738
C10H
-57,25000
45,73473
1,000
-217,8988
103,3988
45,73473
,047
-322,4488
-1,1512
C24H
CS100
CS150
CS200
-161,80000
*
MK10H
-44,26000
45,73473
1,000
-204,9088
116,3888
MK24H
-13,52500
45,73473
1,000
-174,1738
147,1238
K
58,22500
45,73473
1,000
-102,4238
218,8738
CS150
-89,15000
45,73473
1,000
-249,7988
71,4988
CS200
-30,15000
45,73473
1,000
-190,7988
130,4988
C10H
31,90000
45,73473
1,000
-128,7488
192,5488
C24H
-72,65000
45,73473
1,000
-233,2988
87,9988
MK10H
44,89000
45,73473
1,000
-115,7588
205,5388
MK24H
75,62500
45,73473
1,000
-85,0238
236,2738
K
147,37500
45,73473
,102
-13,2738
308,0238
CS100
89,15000
45,73473
1,000
-71,4988
249,7988
CS200
59,00000
45,73473
1,000
-101,6488
219,6488
C10H
-27,10000
45,73473
1,000
-187,7488
133,5488
C24H
-131,65000
45,73473
,231
-292,2988
28,9988
MK10H
-14,11000
45,73473
1,000
-174,7588
146,5388
MK24H
16,62500
45,73473
1,000
-144,0238
177,2738
K
88,37500
45,73473
1,000
-72,2738
249,0238
CS100
30,15000
45,73473
1,000
-130,4988
190,7988
CS150
-59,00000
45,73473
1,000
-219,6488
101,6488
*. The mean difference is significant at the 0.05 level.
Universitas Sumatera Utara
Lampiran 4. Hasil Analisis SPSS 20 untuk Jumlah Eritrosit
ANOVA
Jumlah_Eritrosit
Sum of Squares
Between Groups
Within Groups
df
39960221875000,
7
000
15536975000000,
24
000
55497196875000,
Total
Mean Square
F
5708603125000,0
00
Sig.
8,818
,000
647373958333,33
3
31
000
Multiple Comparisons
Dependent Variable: Jumlah_Eritrosit
Bonferroni
(I)
(J) Perlakuan
Perlakuan
C24H
C10H
Sig.
95% Confidence Interval
Difference (I-
Lower
Upper
J)
Bound
Bound
1,000 -1885952,86
2110952,86
MK10H
-195000,000 568934,952
1,000 -2193452,86
1803452,86
MK24H
1452500,000 568934,952
-545952,86
3450952,86
1,000 -1645952,86
2350952,86
568934,952
,012 -4323452,86
-326547,14
CS150
670000,000 568934,952
1,000 -1328452,86
2668452,86
CS200
-1445000,000 568934,952
,503 -3443452,86
553452,86
C10H
-112500,000 568934,952
1,000 -2110952,86
1885952,86
MK10H
-307500,000 568934,952
1,000 -2305952,86
1690952,86
MK24H
1340000,000 568934,952
K
352500,000 568934,952
2325000,000*
,489
-658452,86
3338452,86
1,000 -1758452,86
2238452,86
568934,952
,007 -4435952,86
-439047,14
CS150
557500,000 568934,952
1,000 -1440952,86
2555952,86
CS200
-1557500,000 568934,952
,321 -3555952,86
440952,86
C10H
195000,000 568934,952
1,000 -1803452,86
2193452,86
C24H
307500,000 568934,952
1,000 -1690952,86
2305952,86
K
CS100
MK10H
Std. Error
112500,000 568934,952
CS100
C24H
Mean
MK24H
K
CS100
CS150
240000,000 568934,952
2437500,000*
1647500,000 568934,952
547500,000 568934,952
2130000,000
*
568934,952
865000,000 568934,952
,757
,222
-350952,86
3645952,86
1,000 -1450952,86
2545952,86
,028
-4128452,86
1,000 -1133452,86
-131547,14
2863452,86
Universitas Sumatera Utara
CS200
-1250000,000 568934,952
1,000 -3248452,86
748452,86
C10H
-1452500,000 568934,952
,489 -3450952,86
545952,86
C24H
-1340000,000 568934,952
,757 -3338452,86
658452,86
MK10H
-1647500,000 568934,952
,222 -3645952,86
350952,86
K
-1100000,000 568934,952
1,000 -3098452,86
898452,86
MK24H
CS100
CS150
,001 -4895952,86
-899047,14
C10H
-352500,000 568934,952
1,000 -2350952,86
1645952,86
C24H
-240000,000 568934,952
1,000 -2238452,86
1758452,86
MK10H
-547500,000 568934,952
1,000 -2545952,86
1450952,86
MK24H
1100000,000 568934,952
1,000
-898452,86
3098452,86
568934,952
,002 -4675952,86
-679047,14
CS150
317500,000 568934,952
1,000 -1680952,86
2315952,86
CS200
-1797500,000 568934,952
,119 -3795952,86
200952,86
C10H
2325000,000
*
568934,952
,012
326547,14
4323452,86
C24H
2437500,000* 568934,952
,007
439047,14
4435952,86
*
2897500,000*
2677500,000*
MK10H
2130000,000
568934,952
,028
131547,14
4128452,86
MK24H
3777500,000* 568934,952
,000
1779047,14
5775952,86
2677500,000
*
568934,952
,002
679047,14
4675952,86
2995000,000
*
568934,952
,001
996547,14
4993452,86
CS150
CS200
880000,000 568934,952
1,000 -1118452,86
2878452,86
C10H
-670000,000 568934,952
1,000 -2668452,86
1328452,86
C24H
-557500,000 568934,952
1,000 -2555952,86
1440952,86
MK10H
-865000,000 568934,952
1,000 -2863452,86
1133452,86
MK24H
782500,000 568934,952
1,000 -1215952,86
2780952,86
-317500,000 568934,952
1,000 -2315952,86
1680952,86
568934,952
,001 -4993452,86
-996547,14
568934,952
,030 -4113452,86
-116547,14
K
CS100
CS200
CS200
-782500,000 568934,952
,000 -5775952,86 -1779047,14
568934,952
K
CS150
568934,952
1215952,86
CS100
CS100
3777500,000*
1,000 -2780952,86
CS200
K
-
2995000,000*
2115000,000*
C10H
1445000,000 568934,952
,503
-553452,86
3443452,86
C24H
1557500,000 568934,952
,321
-440952,86
3555952,86
MK10H
1250000,000 568934,952
1,000
-748452,86
3248452,86
,001
899047,14
4895952,86
MK24H
2897500,000
*
568934,952
Universitas Sumatera Utara
K
1797500,000 568934,952
CS100
-880000,000 568934,952
CS150
2115000,000
*
,119
568934,952
-200952,86
3795952,86
1,000 -2878452,86
1118452,86
,030
116547,14
4113452,86
*. The mean difference is significant at the 0.05 level.
Lampiran 5. Hasil Analisis SPSS 20untuk kadar SGOT
ANOVA
Kadar_SGOT
Sum of Squares
Df
Mean Square
F
Sig.
Between Groups
82447,565
7
11778,224
1,618
,178
Within Groups
174659,750
24
7277,490
Total
257107,315
31
Lampiran 6. Hasil Analisis SPSS 20untuk kadar Hemoglobin
ANOVA
Kadar_Hemoglobin
Sum of Squares
Df
Mean Square
F
Sig.
Between Groups
57,697
7
8,242
1,952
,105
Within Groups
101,363
24
4,223
Total
159,060
31
Lampiran 7. Perhitungan dosis pada tikus (Konversi dosis mencit 20 g ke
tikus 200 g)
No
Perlakuan
Konversi mencit ke tikus
Dosis Tikus
1
Dosis CCl4
0,007 ------7,0
0,007 x 7 = 0,049 mL
2
Dosis Pelarut Minyak kelapa
0,007 ------7,0
0,1 x 7 = 0,7 mL
3
Dosis Larutan
Minyak kelapa
larutan CCl4 0,049 mL
dilarutkan dalam 0,7 mL
larutan minyak kelapa
larutan CCl4 4,9 mL dilarutkan
dalam 70 mL minyak kelapa dan
dosis CCl4 yang diberikan
sebanyak 0,7 mL/200 g BB
CCl4
+
Universitas Sumatera Utara
Lampiran 8. Dosis Ekstrak metanol suren
No Dosis
Dosis I
1
2
3
Dosis 100 mg/kg BB
Dosis II
Dosis 150 mg/kg BB
Dosis III
Dosis 200 mg/kg BB
Perhitungan
Hasil
100 mg/kg BB: x/200 g
20g/200 g BB
150 mg/kg BB : x/200 g
30g/200 g BB
200 mg/kg BB: x/200 g
40g/200 g BB
30 gr dalam 100 mL akuades =0,03 mg dalam 1mL air
Lampiran 9. Tabel Konversi Perhitungan Dosis Untuk Berbagai Jenis (Spesies)
Hewan Uji Menurut Laurence & Bacharah, 1984
Mencit
20 g
Tikus
200 g
Marmut
400 g
Kelinci
1,5 kg
Kucing
2 kg
Kera
4 kg
Anjing
4 kg
Manusia
70 kg
Mencit
20 g
1,0
Tikus
200 g
7,0
Marmut
400 g
13,25
Kelinci
1,5 kg
27,8
Kucing
2 kg
29,7
Anjing
4 kg
64,1
Manusia
70 kg
387,9
0,14
1,0
1,74
3,9
4,2
9,2
56,0
0,08
0,57
1,0
2,25
2,4
5,2
31,5
0,04
0,25
0,44
1,0
1,08
2,4
14,2
0,03
0,23
0,41
0,92
1,0
2,2
13,0
0,016
0,12
0,19
0,42
0,45
1,0
6,1
0,008
0,06
0,10
0,22
0,24
0,52
3,1
0,0026
0,018
0,031
0,07
0,076
0,16
1,0
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Lampiran 10. Pembuatan Ekstrak metanol daun suren
Daun Suren
dicuci
dikering anginkan selama 3 hari
dipotong kecil-kecil dan diblender
Bubuk daun suren
dimasukkan kedalam botol tertutup
direndam dengan metanol
dibiarkan selama dua hari
disaring dengan menggunakan kertas
saring
diuapkan diatas water bath
ekstrak kental metanol suren
dilarutkan dengan etil asetat sampai uji
flavonoid negatif (FeCl3)
Residu (diduga tanin)
Fraksi Etil asetat
diuapkan sampai kering
dilarutkan dengan metanol
Larutan metanol
ekstraksi dengan n-heksan
n-heksan
Larutan
metanol
diuapkan sampai kering
Flavonoid
Total
Universitas Sumatera Utara
Lampiran 11
3.1.Pengambilan darah
Pengambilan darah tikus
Hati Tikus
3.2 Pengukuran Kadar SGPT dan SGOT
Darah EDTA
Mengukur kadar SGPT
3.3 Kadar Hemoglobin dan Penghitungan Jumlah Eritrosit
Darah dalam larutan hayem
Menghitung Eritrosit
Universitas Sumatera Utara
Darah dalam Cyanmethglobin
3.4.Pembuatan Ekstrak Suren dan minyak kelapa
Perendaman Serbuk suren
pada metanol
Skrining fitokimia
Suren pada rotary evaporator
Pembuatan minyak kelapa
Universitas Sumatera Utara
Universitas Sumatera Utara
Lampiran 2. Data Pengamatan Rata-Rata Kadar SGPT, SGOT, Eritrosit
dan Kadar HbTikus putih yang diberi ekstrak metanol suren
dengan dosis 100 mg/kg BB, 150 mg/kg BB,dan 200 mg/kg/BB
Kelompok
Rata-rata Kadar
SGPT(µ/L)
Rata-rata Kadar
SGOT (µ/L)
Rata-rata
Jumlah Eritrosit
(x 106/mm3)
Rata-rata
Kadar Hb
(g/100mL)
C10H
C24H
MK10H
MK24H
K
CS100
CS150
CS200
255,35
359,9
242,36
211,62
139,87
198,1
287,25
228,25
371,47
362,75
343,22
354,97
315,02
272,25
245,5
236,7
7,42
7,31
7,61
5,97
7,07
9,74
6,75
8,86
15,42
12,42
13,85
13,1
11,65
12,47
15,65
13,05
Universitas Sumatera Utara
Lampiran 3. Hasil Analisis SPSS 20 untuk Kadar SGPT
ANOVA
Kadar_SGPT
Sum of Squares
Df
Mean Square
F
Sig.
Between Groups
118290,701
7
16898,672
4,040
,005
Within Groups
100399,936
24
4183,331
Total
218690,638
31
Multiple Comparisons
Dependent Variable: Kadar_SGPT
Bonferroni
(I)
(J) Perlakuan
Perlakuan
C24H
C10H
C24H
MK10H
MK24H
Mean
Std. Error
Sig.
95% Confidence Interval
Difference (I-
Lower
J)
Bound
Upper Bound
-104,55000
45,73473
,879
-265,1988
56,0988
MK10H
12,99000
45,73473
1,000
-147,6588
173,6388
MK24H
43,72500
45,73473
1,000
-116,9238
204,3738
K
115,47500
45,73473
,521
-45,1738
276,1238
CS100
57,25000
45,73473
1,000
-103,3988
217,8988
CS150
-31,90000
45,73473
1,000
-192,5488
128,7488
CS200
27,10000
45,73473
1,000
-133,5488
187,7488
C10H
104,55000
45,73473
,879
-56,0988
265,1988
MK10H
117,54000
45,73473
,470
-43,1088
278,1888
MK24H
148,27500
45,73473
,097
-12,3738
308,9238
220,02500
*
45,73473
,002
59,3762
380,6738
CS100
161,80000
*
45,73473
,047
1,1512
322,4488
CS150
72,65000
45,73473
1,000
-87,9988
233,2988
CS200
131,65000
45,73473
,231
-28,9988
292,2988
C10H
-12,99000
45,73473
1,000
-173,6388
147,6588
C24H
-117,54000
45,73473
,470
-278,1888
43,1088
MK24H
30,73500
45,73473
1,000
-129,9138
191,3838
K
102,48500
45,73473
,968
-58,1638
263,1338
CS100
44,26000
45,73473
1,000
-116,3888
204,9088
CS150
-44,89000
45,73473
1,000
-205,5388
115,7588
CS200
14,11000
45,73473
1,000
-146,5388
174,7588
C10H
-43,72500
45,73473
1,000
-204,3738
116,9238
C24H
-148,27500
45,73473
,097
-308,9238
12,3738
MK10H
-30,73500
45,73473
1,000
-191,3838
129,9138
K
71,75000
45,73473
1,000
-88,8988
232,3988
CS100
13,52500
45,73473
1,000
-147,1238
174,1738
K
Universitas Sumatera Utara
K
CS150
-75,62500
45,73473
1,000
-236,2738
85,0238
CS200
-16,62500
45,73473
1,000
-177,2738
144,0238
C10H
-115,47500
45,73473
,521
-276,1238
45,1738
C24H
-220,02500
*
45,73473
,002
-380,6738
-59,3762
MK10H
-102,48500
45,73473
,968
-263,1338
58,1638
MK24H
-71,75000
45,73473
1,000
-232,3988
88,8988
CS100
-58,22500
45,73473
1,000
-218,8738
102,4238
CS150
-147,37500
45,73473
,102
-308,0238
13,2738
CS200
-88,37500
45,73473
1,000
-249,0238
72,2738
C10H
-57,25000
45,73473
1,000
-217,8988
103,3988
45,73473
,047
-322,4488
-1,1512
C24H
CS100
CS150
CS200
-161,80000
*
MK10H
-44,26000
45,73473
1,000
-204,9088
116,3888
MK24H
-13,52500
45,73473
1,000
-174,1738
147,1238
K
58,22500
45,73473
1,000
-102,4238
218,8738
CS150
-89,15000
45,73473
1,000
-249,7988
71,4988
CS200
-30,15000
45,73473
1,000
-190,7988
130,4988
C10H
31,90000
45,73473
1,000
-128,7488
192,5488
C24H
-72,65000
45,73473
1,000
-233,2988
87,9988
MK10H
44,89000
45,73473
1,000
-115,7588
205,5388
MK24H
75,62500
45,73473
1,000
-85,0238
236,2738
K
147,37500
45,73473
,102
-13,2738
308,0238
CS100
89,15000
45,73473
1,000
-71,4988
249,7988
CS200
59,00000
45,73473
1,000
-101,6488
219,6488
C10H
-27,10000
45,73473
1,000
-187,7488
133,5488
C24H
-131,65000
45,73473
,231
-292,2988
28,9988
MK10H
-14,11000
45,73473
1,000
-174,7588
146,5388
MK24H
16,62500
45,73473
1,000
-144,0238
177,2738
K
88,37500
45,73473
1,000
-72,2738
249,0238
CS100
30,15000
45,73473
1,000
-130,4988
190,7988
CS150
-59,00000
45,73473
1,000
-219,6488
101,6488
*. The mean difference is significant at the 0.05 level.
Universitas Sumatera Utara
Lampiran 4. Hasil Analisis SPSS 20 untuk Jumlah Eritrosit
ANOVA
Jumlah_Eritrosit
Sum of Squares
Between Groups
Within Groups
df
39960221875000,
7
000
15536975000000,
24
000
55497196875000,
Total
Mean Square
F
5708603125000,0
00
Sig.
8,818
,000
647373958333,33
3
31
000
Multiple Comparisons
Dependent Variable: Jumlah_Eritrosit
Bonferroni
(I)
(J) Perlakuan
Perlakuan
C24H
C10H
Sig.
95% Confidence Interval
Difference (I-
Lower
Upper
J)
Bound
Bound
1,000 -1885952,86
2110952,86
MK10H
-195000,000 568934,952
1,000 -2193452,86
1803452,86
MK24H
1452500,000 568934,952
-545952,86
3450952,86
1,000 -1645952,86
2350952,86
568934,952
,012 -4323452,86
-326547,14
CS150
670000,000 568934,952
1,000 -1328452,86
2668452,86
CS200
-1445000,000 568934,952
,503 -3443452,86
553452,86
C10H
-112500,000 568934,952
1,000 -2110952,86
1885952,86
MK10H
-307500,000 568934,952
1,000 -2305952,86
1690952,86
MK24H
1340000,000 568934,952
K
352500,000 568934,952
2325000,000*
,489
-658452,86
3338452,86
1,000 -1758452,86
2238452,86
568934,952
,007 -4435952,86
-439047,14
CS150
557500,000 568934,952
1,000 -1440952,86
2555952,86
CS200
-1557500,000 568934,952
,321 -3555952,86
440952,86
C10H
195000,000 568934,952
1,000 -1803452,86
2193452,86
C24H
307500,000 568934,952
1,000 -1690952,86
2305952,86
K
CS100
MK10H
Std. Error
112500,000 568934,952
CS100
C24H
Mean
MK24H
K
CS100
CS150
240000,000 568934,952
2437500,000*
1647500,000 568934,952
547500,000 568934,952
2130000,000
*
568934,952
865000,000 568934,952
,757
,222
-350952,86
3645952,86
1,000 -1450952,86
2545952,86
,028
-4128452,86
1,000 -1133452,86
-131547,14
2863452,86
Universitas Sumatera Utara
CS200
-1250000,000 568934,952
1,000 -3248452,86
748452,86
C10H
-1452500,000 568934,952
,489 -3450952,86
545952,86
C24H
-1340000,000 568934,952
,757 -3338452,86
658452,86
MK10H
-1647500,000 568934,952
,222 -3645952,86
350952,86
K
-1100000,000 568934,952
1,000 -3098452,86
898452,86
MK24H
CS100
CS150
,001 -4895952,86
-899047,14
C10H
-352500,000 568934,952
1,000 -2350952,86
1645952,86
C24H
-240000,000 568934,952
1,000 -2238452,86
1758452,86
MK10H
-547500,000 568934,952
1,000 -2545952,86
1450952,86
MK24H
1100000,000 568934,952
1,000
-898452,86
3098452,86
568934,952
,002 -4675952,86
-679047,14
CS150
317500,000 568934,952
1,000 -1680952,86
2315952,86
CS200
-1797500,000 568934,952
,119 -3795952,86
200952,86
C10H
2325000,000
*
568934,952
,012
326547,14
4323452,86
C24H
2437500,000* 568934,952
,007
439047,14
4435952,86
*
2897500,000*
2677500,000*
MK10H
2130000,000
568934,952
,028
131547,14
4128452,86
MK24H
3777500,000* 568934,952
,000
1779047,14
5775952,86
2677500,000
*
568934,952
,002
679047,14
4675952,86
2995000,000
*
568934,952
,001
996547,14
4993452,86
CS150
CS200
880000,000 568934,952
1,000 -1118452,86
2878452,86
C10H
-670000,000 568934,952
1,000 -2668452,86
1328452,86
C24H
-557500,000 568934,952
1,000 -2555952,86
1440952,86
MK10H
-865000,000 568934,952
1,000 -2863452,86
1133452,86
MK24H
782500,000 568934,952
1,000 -1215952,86
2780952,86
-317500,000 568934,952
1,000 -2315952,86
1680952,86
568934,952
,001 -4993452,86
-996547,14
568934,952
,030 -4113452,86
-116547,14
K
CS100
CS200
CS200
-782500,000 568934,952
,000 -5775952,86 -1779047,14
568934,952
K
CS150
568934,952
1215952,86
CS100
CS100
3777500,000*
1,000 -2780952,86
CS200
K
-
2995000,000*
2115000,000*
C10H
1445000,000 568934,952
,503
-553452,86
3443452,86
C24H
1557500,000 568934,952
,321
-440952,86
3555952,86
MK10H
1250000,000 568934,952
1,000
-748452,86
3248452,86
,001
899047,14
4895952,86
MK24H
2897500,000
*
568934,952
Universitas Sumatera Utara
K
1797500,000 568934,952
CS100
-880000,000 568934,952
CS150
2115000,000
*
,119
568934,952
-200952,86
3795952,86
1,000 -2878452,86
1118452,86
,030
116547,14
4113452,86
*. The mean difference is significant at the 0.05 level.
Lampiran 5. Hasil Analisis SPSS 20untuk kadar SGOT
ANOVA
Kadar_SGOT
Sum of Squares
Df
Mean Square
F
Sig.
Between Groups
82447,565
7
11778,224
1,618
,178
Within Groups
174659,750
24
7277,490
Total
257107,315
31
Lampiran 6. Hasil Analisis SPSS 20untuk kadar Hemoglobin
ANOVA
Kadar_Hemoglobin
Sum of Squares
Df
Mean Square
F
Sig.
Between Groups
57,697
7
8,242
1,952
,105
Within Groups
101,363
24
4,223
Total
159,060
31
Lampiran 7. Perhitungan dosis pada tikus (Konversi dosis mencit 20 g ke
tikus 200 g)
No
Perlakuan
Konversi mencit ke tikus
Dosis Tikus
1
Dosis CCl4
0,007 ------7,0
0,007 x 7 = 0,049 mL
2
Dosis Pelarut Minyak kelapa
0,007 ------7,0
0,1 x 7 = 0,7 mL
3
Dosis Larutan
Minyak kelapa
larutan CCl4 0,049 mL
dilarutkan dalam 0,7 mL
larutan minyak kelapa
larutan CCl4 4,9 mL dilarutkan
dalam 70 mL minyak kelapa dan
dosis CCl4 yang diberikan
sebanyak 0,7 mL/200 g BB
CCl4
+
Universitas Sumatera Utara
Lampiran 8. Dosis Ekstrak metanol suren
No Dosis
Dosis I
1
2
3
Dosis 100 mg/kg BB
Dosis II
Dosis 150 mg/kg BB
Dosis III
Dosis 200 mg/kg BB
Perhitungan
Hasil
100 mg/kg BB: x/200 g
20g/200 g BB
150 mg/kg BB : x/200 g
30g/200 g BB
200 mg/kg BB: x/200 g
40g/200 g BB
30 gr dalam 100 mL akuades =0,03 mg dalam 1mL air
Lampiran 9. Tabel Konversi Perhitungan Dosis Untuk Berbagai Jenis (Spesies)
Hewan Uji Menurut Laurence & Bacharah, 1984
Mencit
20 g
Tikus
200 g
Marmut
400 g
Kelinci
1,5 kg
Kucing
2 kg
Kera
4 kg
Anjing
4 kg
Manusia
70 kg
Mencit
20 g
1,0
Tikus
200 g
7,0
Marmut
400 g
13,25
Kelinci
1,5 kg
27,8
Kucing
2 kg
29,7
Anjing
4 kg
64,1
Manusia
70 kg
387,9
0,14
1,0
1,74
3,9
4,2
9,2
56,0
0,08
0,57
1,0
2,25
2,4
5,2
31,5
0,04
0,25
0,44
1,0
1,08
2,4
14,2
0,03
0,23
0,41
0,92
1,0
2,2
13,0
0,016
0,12
0,19
0,42
0,45
1,0
6,1
0,008
0,06
0,10
0,22
0,24
0,52
3,1
0,0026
0,018
0,031
0,07
0,076
0,16
1,0
Universitas Sumatera Utara
Lampiran 10. Pembuatan Ekstrak metanol daun suren
Daun Suren
dicuci
dikering anginkan selama 3 hari
dipotong kecil-kecil dan diblender
Bubuk daun suren
dimasukkan kedalam botol tertutup
direndam dengan metanol
dibiarkan selama dua hari
disaring dengan menggunakan kertas
saring
diuapkan diatas water bath
ekstrak kental metanol suren
dilarutkan dengan etil asetat sampai uji
flavonoid negatif (FeCl3)
Residu (diduga tanin)
Fraksi Etil asetat
diuapkan sampai kering
dilarutkan dengan metanol
Larutan metanol
ekstraksi dengan n-heksan
n-heksan
Larutan
metanol
diuapkan sampai kering
Flavonoid
Total
Universitas Sumatera Utara
Lampiran 11
3.1.Pengambilan darah
Pengambilan darah tikus
Hati Tikus
3.2 Pengukuran Kadar SGPT dan SGOT
Darah EDTA
Mengukur kadar SGPT
3.3 Kadar Hemoglobin dan Penghitungan Jumlah Eritrosit
Darah dalam larutan hayem
Menghitung Eritrosit
Universitas Sumatera Utara
Darah dalam Cyanmethglobin
3.4.Pembuatan Ekstrak Suren dan minyak kelapa
Perendaman Serbuk suren
pada metanol
Skrining fitokimia
Suren pada rotary evaporator
Pembuatan minyak kelapa
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