characrerization of vector DNA microsatellite dengue hemorragic fever(DHF) aedes aegypti with enrichment method.

Characterization of vector DNA microsatellite Dengue Hemorragic
Fever {DHF) Aedes aeglpti with Enricharent Method

I

Hasmiwatil, Diong l{on'ljong2 and Dessy Arisanty 3
Pa.asitology Divisioru Medical F'aculiy. Andalas Llniversily, Padang

gmail. co m
and Science Faculty,
Andalas University, Padang
lBiochernestry Division, Medical lraculty, AndrJas
University, Padang
Emai I : hasmiwati6

5

@,
t Biology Departement, Mathemaics

ABSTRACT

This study aimed to characterizr DNA microsatellite of' Aedes aeg,tpti with enrichment
method, DNA was extracted and then, DNA was digested with restricted enzyme
simultaneously with Alu I, Rsal and Hinc II. DNA was adtrered with MluI adaptor (consist of
21 - mer: 5' CTC ffc CTT ACG CGT GGA C'tA3' and phospcrilated with 25-mer in end of
5': 3'ACA CGA GAA CGA A TG GCA CC'l'GAI'p 5'). Six oligonucleotides have (Allzo,
(CTbo {CTho'(AC}20 (AG}zo and (GC):o) microsatellite motifs, and hybrid by using Hybon
membrane. Result fragments from hybridization were eluled and amplified eCR) with 21mer primer. Result of study showed there were 700 bp,610 bp, 550 bp,490 bp dan 450 bp
'lhey were DNA fragment candidatef which
products of amplification from eluted DNA.
conrained motif of microsatellite. We concluded that enrichment method could result DNA
microsatellite fragment candidates' of DIIF Aedes aeppti vcctor in Wbst Sumatera"
Key Word: Aedes aegtpti,DNA extracl enrichrnent- hybridiz-ation, and DNA microsatelfite