Respon Cylindrocladium sp. Terhadap Fungisida Berbahan Aktif Mancozeb Secara In Vitro

ABSTRACT

PEBRIAN INDRA RISKY DALIMUNTHE. Response Cylindrocladium sp. To
the active substance Mancozeb Fungicide In Vitro. Guided by EDY BATARA
MULYA SIREGAR and NELLY ANNA.
Cylindrocladium sp. is one of the pathogens trigger dangerous diseases
that blight on eucalyptus plants in the pulp industry. Widely spread and
prevention with the use of a contact fungicide mancozeb 80% WP.
Research on Response Cylindrocladium sp. To the active
substanceMancozeb Fungicide In Vitro has been completed. The purpose of this
study was to determine the response Cylindrocladium sp. on the provision of
different concentrations and characterize the growth of colonies (shape, color, and
texture). Research using completely randomized design (CRD) non factorial with
basic media PDA. Concentrations used were 0 mg / ml (control) 0.4 mg / ml
(M1), 0.8 mg / ml (M2), 1.2 mg / ml (M3), and 1.6 mg / ml (M4). Parameters
measured were Cylindrocladium colony diameter sp., The percentage of colonies
relative barriers Cylindrocladium sp., Spore density Cylindrocladium sp.,
Macroscopic and microscopic observation. Treatment significantly affect colony
growth, relative barriers and spore density. Macroscopic characteristics include a
white colony, the hyphae such as cotton, spread evenly and thicken in the middle.
Microscopic characteristics which include changes in the structure of the hyphae,

branching hyphae, density klamidiospora, Konia increasingly stunted and swelling
of
such
tumors
in
a
set
of
hyphae.
Keywords: Response, In Vitro, Cylindrocladium sp., Fungicide Mancozeb 80%
WP.

Universitas Sumatera Utara

ABSTRAK

PEBRIAN INDRA RISKY DALIMUNTHE. Respon Cylindrocladium sp.
Terhadap Fungisida Berbahan Aktif Mancozeb Secara In Vitro. Dibimbing oleh
EDY BATARA MULYA SIREGAR dan NELLY ANNA
Cylindrocladium sp. adalah salah satu patogen pemicu munculnya

penyakit berbahaya yaitu hawar daun pada tanaman ekaliptus pada industri pulp.
Penyebarannya secara luas dan pencegahannya dengan penggunaan fungisida
kontak mancozeb 80% WP.
Penelitian tentang Respon Cylindrocladium sp. Terhadap Fungisida
Berbahan Aktif Mancozeb Secara In Vitro telah selesai dilakukan. Tujuan
penelitian ini adalah menentukan respon Cylindrocladium sp. terhadap pemberian
konsentrasi yang berbeda dan mengkarakterisasi pertumbuhan koloni (bentuk,
warna, dan tekstur). Penelitian menggunakan Rancangan Acak Lengkap (RAL)
non Faktorial dengan media dasar PDA. Konsentrasi yang digunakan adalah 0
mg/ml (kontrol) 0.4 mg/ml (M1), 0.8 mg/ml (M2), 1.2 mg/ml (M3), dan 1.6
mg/ml (M4). Parameter yang diamati adalah diameter koloni Cylindrocladium sp.,
Persentase hambatan relative koloni Cylindrocladium sp., kerapatan spora
Cylindrocladium sp., pengamatan makroskopis dan mikroskopis. Perlakuan
berpengaruh nyata terhadap pertumbuhan koloni, hambatan relatif dan kerapatan
spora. Karakteristik makroskopis meliputi koloni berwarna putih, hifa seperti
kapas, menyebar merata dan menebal di tengah. Karakteristik mikroskopis yang
meliputi perubahan struktur pada hifa, percabangan hifa, kerapatan klamidiospora,
konia yang semakin kerdil dan terjadi pembengkakan seperti tumor pada
sekumpulan hifa.
Kata kunci: Respon, In Vitro, Cylindrocladium sp., Fungisida Mancozeb 80% WP.

Universitas Sumatera Utara