Respon Cylindrocladium sp. Terhadap Fungisida Berbahan Aktif Mancozeb Secara In Vitro
LAMPIRAN
Lampiran 1. Data Diameter Koloni Cylindrocladium sp.
Tabel 1. Pengamatan I Hari ke-4
Perlakuan
Ulangan
Total
Rataan
1
2
3
4
5
M0(kontrol)
2.19
2.31
2.3
2.27
2.36
11.43
2.286
M1(0.4 mg/ml)
2.25
2.17
1.89
2.32
2.1
10.73
2.146
M2(0.8 mg/ml)
2.02
2.01
2.19
1.95
2.33
10.5
2.1
M3(1.2 mg/ml)
2.52
2.06
1.88
2.05
2.18
10.69
2.138
M4(1.6 mg/ml)
2.07
1.96
1.92
2.04
2.16
10.15
2.03
Total
53.5
Rataan
2.14
Tabel 2. Analisis Sidik Ragam Pengamatan I Hari ke-4
SK
DB
JK
KT
Perlakuan
4
0.1752
0.04382
Galat
20
0.4867
0.02433
Total
24
0.662
Tabel 3. Pengamatan II Hari ke-8
Perlakuan
F Tabel
F Tabel
0.05
0.01
F Hitung
1.80062
tn
2.866081
Ulangan
4.43069016
Total
Rataan
1
2
3
4
5
M0(kontrol)
3.72
4.88
5.85
5.15
4.64
24.24
4.848
M1(0.4 mg/ml)
5.21
4.51
3.52
4.88
5.81
23.93
4.786
M2(0.8 mg/ml)
3.55
3.47
3.17
3.18
4.23
17.6
3.52
M3(1.2 mg/ml)
2.14
3.11
8.2
3.35
3.12
19.92
3.984
M4(1.6 mg/ml)
2.46
2.91
3.23
3.43
1.98
14.01
2.802
Total
99.7
Rataan
3.988
Tabel 4. Analisis Sidik Ragam Pengamatan II Hari ke-8
SK
DB
JK
KT
Perlakuan
4
15.010
3.75255
Galat
20
30.542
1.52714
Total
24
45.553
F Tabel
F Tabel
0.05
0.01
F Hitung
2.457240
tn
2.866081
4.4306901
Universitas Sumatera Utara
Tabel 5. Pengamatan III Hari ke-12
Perlakuan
Ulangan
Total
Rataan
1
2
3
4
5
M0(kontrol)
5.41
7.27
8.01
7.79
7.17
35.65
7.13
M1(0.4 mg/ml)
5.92
7.41
5.78
7.81
8.48
35.4
7.08
M2(0.8 mg/ml)
3.41
6.09
3.75
4.06
4.05
21.36
4.272
M3(1.2 mg/ml)
2.79
2.82
7.85
2.92
4.49
20.87
4.174
M4(1.6 mg/ml)
3.59
5.52
3.06
4.01
1.97
18.15
3.63
Total
131.43
Rataan
5.2572
Tabel 6. Analisis Sidik Ragam Pengamatan III Hari ke-12
SK
DB
JK
KT
F Tabel
F Tabel
0.05
0.01
F Hitung
Perlakuan
4
58.108
14.5271
Galat
20
39.974
1.99873
Total
24
98.083
7.26817
**
2.8660814
4.4306901
ANOVA
Diameter
Sum of Squares
df
Mean Square
Between Groups
58.109
4
14.527
Within Groups
39.975
20
1.999
Total
98.083
24
Tabel 7. Pengamatan IV Hari ke-16
Perlakuan
1
M0(kontrol)
6.69
F
Sig.
7.268
Ulangan
.001
Total
Rataan
2
3
4
5
8.62
8.43
8.31
7.72
39.77
7.954
M1(0.4 mg/ml)
6.35
7.79
5.83
7.87
8.54
36.38
7.276
M2(0.8 mg/ml)
3.35
6.17
3.8
3.92
3.92
21.16
4.232
M3(1.2 mg/ml)
2.87
2.73
7.75
2.84
4.66
20.85
4.17
M4(1.6 mg/ml)
3.61
5.52
3.02
3.85
2
18
3.6
Total
Rataan
136.16
5.4464
Universitas Sumatera Utara
Tabel 8. Analisis Sidik Ragam Pengamatan IV Hari ke-16
SK
DB
JK
KT
F Hitung
Perlakuan
4
80.743
20.185
Galat
20
37.761
1.888
Total
24
118.505
10.691
**
F Tabel
F Tabel
0.05
0.01
2.8660814
4.4306901
ANOVA
Diameter
Sum of Squares
df
Mean Square
Between Groups
80.743
4
20.186
Within Groups
37.762
20
1.888
118.505
24
Total
Tabel 9. Pengamatan V Hari ke-20
Perlakuan
F
Sig.
10.691
Ulangan
.000
Total
Rataan
1
2
3
4
5
M0(kontrol)
7.87
8.88
8.96
8.79
7.99
42.49
8.498
M1(0.4 mg/ml)
6.37
7.69
6.2
7.98
8.55
36.79
7.358
M2(0.8 mg/ml)
3.03
5.77
3.61
3.61
3.68
19.7
3.94
M3(1.2 mg/ml)
2.61
2.44
7.6
2.54
3.93
19.12
3.824
M4(1.6 mg/ml)
3.32
5.21
2.77
3.47
2.72
17.49
3.498
Total
135.59
Rataan
5.4236
Tabel 10. Analisis Sidik Ragam Pengamatan V Hari ke-20
SK
DB
JK
KT
Perlakuan
4
108.308
27.077
Galat
20
33.2006
1.6600
Total
24
141.508
F Hitung
16.311
*
F Tabel
F Tabel
0.05
0.01
2.8660814
4.4306901
ANOVA
Diameter
Sum of Squares
Between Groups
Within Groups
Total
df
Mean Square
108.308
4
27.077
33.201
20
1.660
141.508
24
F
16.311
Sig.
.000
Universitas Sumatera Utara
LAMPIRAN
Lampiran 2. Data Hambatan Relatif Koloni Cylindrocladium sp.
Tabel 1. Pengamatan Hambatan Relatif Koloni Cylindrocladium sp.
Perlakuan
Ulangan
Total
Rataan
4
8
12
16
20
M0(kontrol)
0
0
0
0
0
0
0
M1(0.4 mg/ml)
6.11
1.24
0.7
8.43
13.31
29.79
5.958
M2(0.8 mg/ml)
8.29
27.42
40.11
46.79
53.59
176.2
35.24
M3(1.2 mg/ml)
6.55
17.94
41.52
47.55
55.01
168.57
33.714
M4(1.6 mg/ml)
11.35
42.27
49.09
54.72
58.72
216.15
43.23
Total
590.71
Rataan
23.6284
Tabel 2. Sidik Ragam Pengamatan Hambatan Relatif Koloni Cylindrocladium sp.
SK
DB
JK
KT
F Tabel
F Tabel
0.05
0.01
F Hitung
Perlakuan
4
7456.58
1864.14
Galat
20
4507.53
225.377
Total
24
11964.1
8.27124
** 2.866081402
4.430690162
ANOVA
Hambatan Relatif
Sum of Squares
df
Mean Square
Between Groups
7456.578
4
1864.145
Within Groups
4507.535
20
225.377
11964.113
24
Total
F
8.271
Sig.
.000
LAMPIRAN
Lampiran 3. Pengamatan Makroskopis Cylindrocladium sp.
Universitas Sumatera Utara
(a)
(b)
(c)
(d)
(e)
Gambar 1. Koloni Fungi Cylindrocladium sp. pada Hari ke-4 Setelah Inokulasi
Keterangan: (a). Kontrol (cm), (b). Konsentrasi 0.4 mg/ml (cm), (c). Konsentrasi 0.8 mg/ml (cm),
(d).Konsentrasi 1.2 mg/ml (cm), dan (e). Konsentrasi 1.6 mg/ml (cm).
(a)
(b)
(c)
(d)
(e)
Gambar 2. Koloni Fungi Cylindrocladium sp. pada Hari ke-8 Setelah Inokulasi
Keterangan: (a). Kontrol (cm), (b). Konsentrasi 0.4 mg/ml (cm), (c). Konsentrasi 0.8 mg/ml (cm),
(d).Konsentrasi 1.2 mg/ml (cm), dan (e). Konsentrasi 1.6 mg/ml (cm).
Universitas Sumatera Utara
(a)
(b)
(c)
(d)
(e)
Gambar 3. Koloni Fungi Cylindrocladium sp. pada Hari ke-12 Setelah Inokulasi
Keterangan: (a). Kontrol (cm), (b). Konsentrasi 0.4 mg/ml (cm), (c). Konsentrasi 0.8 mg/ml (cm),
(d).Konsentrasi 1.2 mg/ml (cm), dan (e). Konsentrasi 1.6 mg/ml (cm).
(a)
(b)
(c)
(d)
(e)
Gambar 4. Koloni Fungi Cylindrocladium sp. pada Hari ke-16 Setelah Inokulasi
Keterangan: (a) Kontrol (cm), (b) Konsentrasi 0.4 mg/ml (cm), (c). Konsentrasi 0.8 mg/ml (cm),
(d). Konsentrasi 1.2 mg/ml (cm), dan (e). Konsentrasi 1.6 mg/ml (cm)
Universitas Sumatera Utara
Lampiran 1. Data Diameter Koloni Cylindrocladium sp.
Tabel 1. Pengamatan I Hari ke-4
Perlakuan
Ulangan
Total
Rataan
1
2
3
4
5
M0(kontrol)
2.19
2.31
2.3
2.27
2.36
11.43
2.286
M1(0.4 mg/ml)
2.25
2.17
1.89
2.32
2.1
10.73
2.146
M2(0.8 mg/ml)
2.02
2.01
2.19
1.95
2.33
10.5
2.1
M3(1.2 mg/ml)
2.52
2.06
1.88
2.05
2.18
10.69
2.138
M4(1.6 mg/ml)
2.07
1.96
1.92
2.04
2.16
10.15
2.03
Total
53.5
Rataan
2.14
Tabel 2. Analisis Sidik Ragam Pengamatan I Hari ke-4
SK
DB
JK
KT
Perlakuan
4
0.1752
0.04382
Galat
20
0.4867
0.02433
Total
24
0.662
Tabel 3. Pengamatan II Hari ke-8
Perlakuan
F Tabel
F Tabel
0.05
0.01
F Hitung
1.80062
tn
2.866081
Ulangan
4.43069016
Total
Rataan
1
2
3
4
5
M0(kontrol)
3.72
4.88
5.85
5.15
4.64
24.24
4.848
M1(0.4 mg/ml)
5.21
4.51
3.52
4.88
5.81
23.93
4.786
M2(0.8 mg/ml)
3.55
3.47
3.17
3.18
4.23
17.6
3.52
M3(1.2 mg/ml)
2.14
3.11
8.2
3.35
3.12
19.92
3.984
M4(1.6 mg/ml)
2.46
2.91
3.23
3.43
1.98
14.01
2.802
Total
99.7
Rataan
3.988
Tabel 4. Analisis Sidik Ragam Pengamatan II Hari ke-8
SK
DB
JK
KT
Perlakuan
4
15.010
3.75255
Galat
20
30.542
1.52714
Total
24
45.553
F Tabel
F Tabel
0.05
0.01
F Hitung
2.457240
tn
2.866081
4.4306901
Universitas Sumatera Utara
Tabel 5. Pengamatan III Hari ke-12
Perlakuan
Ulangan
Total
Rataan
1
2
3
4
5
M0(kontrol)
5.41
7.27
8.01
7.79
7.17
35.65
7.13
M1(0.4 mg/ml)
5.92
7.41
5.78
7.81
8.48
35.4
7.08
M2(0.8 mg/ml)
3.41
6.09
3.75
4.06
4.05
21.36
4.272
M3(1.2 mg/ml)
2.79
2.82
7.85
2.92
4.49
20.87
4.174
M4(1.6 mg/ml)
3.59
5.52
3.06
4.01
1.97
18.15
3.63
Total
131.43
Rataan
5.2572
Tabel 6. Analisis Sidik Ragam Pengamatan III Hari ke-12
SK
DB
JK
KT
F Tabel
F Tabel
0.05
0.01
F Hitung
Perlakuan
4
58.108
14.5271
Galat
20
39.974
1.99873
Total
24
98.083
7.26817
**
2.8660814
4.4306901
ANOVA
Diameter
Sum of Squares
df
Mean Square
Between Groups
58.109
4
14.527
Within Groups
39.975
20
1.999
Total
98.083
24
Tabel 7. Pengamatan IV Hari ke-16
Perlakuan
1
M0(kontrol)
6.69
F
Sig.
7.268
Ulangan
.001
Total
Rataan
2
3
4
5
8.62
8.43
8.31
7.72
39.77
7.954
M1(0.4 mg/ml)
6.35
7.79
5.83
7.87
8.54
36.38
7.276
M2(0.8 mg/ml)
3.35
6.17
3.8
3.92
3.92
21.16
4.232
M3(1.2 mg/ml)
2.87
2.73
7.75
2.84
4.66
20.85
4.17
M4(1.6 mg/ml)
3.61
5.52
3.02
3.85
2
18
3.6
Total
Rataan
136.16
5.4464
Universitas Sumatera Utara
Tabel 8. Analisis Sidik Ragam Pengamatan IV Hari ke-16
SK
DB
JK
KT
F Hitung
Perlakuan
4
80.743
20.185
Galat
20
37.761
1.888
Total
24
118.505
10.691
**
F Tabel
F Tabel
0.05
0.01
2.8660814
4.4306901
ANOVA
Diameter
Sum of Squares
df
Mean Square
Between Groups
80.743
4
20.186
Within Groups
37.762
20
1.888
118.505
24
Total
Tabel 9. Pengamatan V Hari ke-20
Perlakuan
F
Sig.
10.691
Ulangan
.000
Total
Rataan
1
2
3
4
5
M0(kontrol)
7.87
8.88
8.96
8.79
7.99
42.49
8.498
M1(0.4 mg/ml)
6.37
7.69
6.2
7.98
8.55
36.79
7.358
M2(0.8 mg/ml)
3.03
5.77
3.61
3.61
3.68
19.7
3.94
M3(1.2 mg/ml)
2.61
2.44
7.6
2.54
3.93
19.12
3.824
M4(1.6 mg/ml)
3.32
5.21
2.77
3.47
2.72
17.49
3.498
Total
135.59
Rataan
5.4236
Tabel 10. Analisis Sidik Ragam Pengamatan V Hari ke-20
SK
DB
JK
KT
Perlakuan
4
108.308
27.077
Galat
20
33.2006
1.6600
Total
24
141.508
F Hitung
16.311
*
F Tabel
F Tabel
0.05
0.01
2.8660814
4.4306901
ANOVA
Diameter
Sum of Squares
Between Groups
Within Groups
Total
df
Mean Square
108.308
4
27.077
33.201
20
1.660
141.508
24
F
16.311
Sig.
.000
Universitas Sumatera Utara
LAMPIRAN
Lampiran 2. Data Hambatan Relatif Koloni Cylindrocladium sp.
Tabel 1. Pengamatan Hambatan Relatif Koloni Cylindrocladium sp.
Perlakuan
Ulangan
Total
Rataan
4
8
12
16
20
M0(kontrol)
0
0
0
0
0
0
0
M1(0.4 mg/ml)
6.11
1.24
0.7
8.43
13.31
29.79
5.958
M2(0.8 mg/ml)
8.29
27.42
40.11
46.79
53.59
176.2
35.24
M3(1.2 mg/ml)
6.55
17.94
41.52
47.55
55.01
168.57
33.714
M4(1.6 mg/ml)
11.35
42.27
49.09
54.72
58.72
216.15
43.23
Total
590.71
Rataan
23.6284
Tabel 2. Sidik Ragam Pengamatan Hambatan Relatif Koloni Cylindrocladium sp.
SK
DB
JK
KT
F Tabel
F Tabel
0.05
0.01
F Hitung
Perlakuan
4
7456.58
1864.14
Galat
20
4507.53
225.377
Total
24
11964.1
8.27124
** 2.866081402
4.430690162
ANOVA
Hambatan Relatif
Sum of Squares
df
Mean Square
Between Groups
7456.578
4
1864.145
Within Groups
4507.535
20
225.377
11964.113
24
Total
F
8.271
Sig.
.000
LAMPIRAN
Lampiran 3. Pengamatan Makroskopis Cylindrocladium sp.
Universitas Sumatera Utara
(a)
(b)
(c)
(d)
(e)
Gambar 1. Koloni Fungi Cylindrocladium sp. pada Hari ke-4 Setelah Inokulasi
Keterangan: (a). Kontrol (cm), (b). Konsentrasi 0.4 mg/ml (cm), (c). Konsentrasi 0.8 mg/ml (cm),
(d).Konsentrasi 1.2 mg/ml (cm), dan (e). Konsentrasi 1.6 mg/ml (cm).
(a)
(b)
(c)
(d)
(e)
Gambar 2. Koloni Fungi Cylindrocladium sp. pada Hari ke-8 Setelah Inokulasi
Keterangan: (a). Kontrol (cm), (b). Konsentrasi 0.4 mg/ml (cm), (c). Konsentrasi 0.8 mg/ml (cm),
(d).Konsentrasi 1.2 mg/ml (cm), dan (e). Konsentrasi 1.6 mg/ml (cm).
Universitas Sumatera Utara
(a)
(b)
(c)
(d)
(e)
Gambar 3. Koloni Fungi Cylindrocladium sp. pada Hari ke-12 Setelah Inokulasi
Keterangan: (a). Kontrol (cm), (b). Konsentrasi 0.4 mg/ml (cm), (c). Konsentrasi 0.8 mg/ml (cm),
(d).Konsentrasi 1.2 mg/ml (cm), dan (e). Konsentrasi 1.6 mg/ml (cm).
(a)
(b)
(c)
(d)
(e)
Gambar 4. Koloni Fungi Cylindrocladium sp. pada Hari ke-16 Setelah Inokulasi
Keterangan: (a) Kontrol (cm), (b) Konsentrasi 0.4 mg/ml (cm), (c). Konsentrasi 0.8 mg/ml (cm),
(d). Konsentrasi 1.2 mg/ml (cm), dan (e). Konsentrasi 1.6 mg/ml (cm)
Universitas Sumatera Utara