Keanekaragaman Ikan dan Hubungannya dengan Kualitas Air di Perairan Sungai Bingei, Binjai

46

Lampiran 1. Peta Lokasi Penelitian

Keterangan
Stasiun 1
Stasiun 2
Stasiun 3

: Daerah Kontrol
: Daerah Pengerukan Pasir
: Daerah Perkebunan

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Lampiran 2.

Bagan Kerja Metode Winkler untuk Mengukur Kelarutan

Oksigen (DO)
Sampel Air
1 ml MnSO4
1 ml KOH – KI

dikocok
didiamkan
Sampel Dengan
Endapan Putih/Coklat
1 ml H2SO4

dikocok
didiamkan
Larutan Sampel
Berwarna Coklat
diambil sebanyak 100 ml
ditetesi Na2S2O3 0,0125 N

Sampel Berwarna
Kuning Pucat

ditambahkan 5 tetes amilum

Sampel Berwarna
Biru
dititrasi dengan Na2S2O3 0,0125 N

Sampel Bening
Dihitung volume Na2S2O3 yang terpakai
(= nilai DO akhir)
Hasil
(Suin, 2002)

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Lampiran 3. Bagan Kerja Metode Winkler untuk Mengukur BOD 5

Sampel Air


Sampel Air

Sampel Air
diinkubasi selama 5 hari
pada temperatur 20°C

dihitung nilai DO awal

dihitung nilai DO akhir

DO Akhir

DO Awal

Keterangan :



Penghitungan nilai DO awal dan DO akhir sama

dengan penghitungan Nilai DO
Nilai BOD5 = Nilai awal – Nilai DO akhir
(Suin, 2002)

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Lampiran 4. Tabel Kelarutan O2 (Oksigen)
T˚C
0
1
2
3
4
5
6
7
8

9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28

29
30

0,0

0,1

0,2

0,3

0,4

0,5

0,6

0,7

0,8


0,9

14,6
13,77
13,40
13,05
12,70
12,37
12,06
11,76
11,47
11,19
10,92
10,67
10,43
10,20
9,98
9,76
9,56

9,37
9,18
9,01
8,84
8,68
8,53
8,38
8,25
8,11
7,99
7,86
7,75
7,64
7,53

14,12
13,74
13,37
13,01
12,67

12,34
12,03
11,73
11,44
11,16
10,90
10,65
10,40
10,17
9,95
9,74
9,54
9,35
9,18
8,99
8,83
8,67
8,52
8,37
8,23

8,10
7,97
7,85
7,74
7,62
7,52

14,08
13,70
13,33
12,98
12,64
12,31
12,00
11,70
11,41
11,14
10,87
10,62
10,38

10,15
9,93
9,72
9,52
9,33
9,15
8,98
8,81
8,65
8,50
8,36
8,22
8,09
7,96
7,84
7,72
7,61
7,51

14,04
13,66
13,30
12,94
12,60
12,28
11,97
11,67
11,38
11,11
10,85
10,60
10,36
10,13
9,91
9,70
9,50
9,31
9,13
8,96
8,79
8,64
8,49
8,34
8,21
8,07
7,95
7,83
7,71
7,60
7,50

14,00
13,63
13,26
12,91
12,57
12,25
11,94
11,64
11,36
11,08
10,82
10,57
10,34
10,11
9,89
9,68
9,48
9,30
9,12
8,94
8,78
8,62
8,47
8,33
8,19
8,06
7,94
7,82
7,70
7,59
7,48

13,97
13,59
13,22
12,87
12,54
12,22
11,91
11,61
11,33
11,06
10,80
10,55
10,31
10,09
9,87
9,66
9,46
9,28
9,10
8,93
8,76
8,61
8,46
8,32
8,18
8,05
7,92
7,81
7,69
7,58
7,47

13,93
13,55
13,19
12,84
12,51
12,18
11,88
11,58
11,30
11,03
10,77
10,53
10,29
10,06
9,85
9,64
9,45
9,26
9,08
8,91
8,75
8,59
8,44
8,30
8,17
8,04
7,91
7,79
7,68
7,57
7,46

13,89
13,51
13,15
12,81
12,47
12,15
11,85
11,55
11,27
11,00
10,75
10,50
10,27
10,04
9,83
9,62
9,43
9,24
9,06
8,89
58,73
8,58
8,43
8,29
8,15
8,02
7,90
7,78
7,67
7,56
7,45

13,85
13,48
13,12
12,77
12,44
12,12
11,82
11,52
11,25
10,98
10,72
10,48
10,24
10,02
9,81
9,60
9,41
9,22
9,04
8,88
8,71
8,56
8,41
8,27
8,14
8,01
7,89
7,77
7,66
7,55
7,44

13,81
13,44
13,08
12,74
12,41
12,09
11,79
11,50
11,22
10,95
10,70
10,45
10,22
10,00
9,78
9,58
9,39
9,20
9,03
8,86
8,70
8,55
8,40
8,26
8,13
8,00
7,88
7,76
7,65
7,54
7,43

(Barus, 2004)

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Lampiran 5. Bagan Kerja Pengukuran Nitrat (NO 3 )
5 ml Sampel Air
1 ml NaCl (pipet volum)
5 ml H 2 SO 4
4 tetes Brucine Sulfat
Sulfanic acid

Larutan

Dipanaskan selama 25 menit

Larutan
Didinginkan
Diukur dengan
Spektrofotometer pada λ =
410 nm
Hasil

\

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Lampiran 5. Bagan Kerja Pengukuran Fosfat (PO 4 3-)
5 ml Sampel Air
1 ml Amstrong Reagent
1 ml Ascorbic Acid

Larutan
Dibiarka selama 20 menit
Diukur dengan
Spektrofotometer pada λ =
880 nm
Hasil

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Lampiran 7. Panjang dan Berat Ikan
a. Ikan Nila (Oreochromis niloticus)

No

Length (L)

Weight (W)

Log L

0.50514998

Log L*Log
W
0.400276645

1

6.2

3.2

0.79239169

0.62788459

2

5

2.3

0.69897

0.36172784

0.252836907

0.48855907

3

5.2

2.6

0.71600334

0.41497335

0.297122305

0.51266079

4

13.5

47.2

1.13033377

1.673942

1.892113168

1.27765443

5

12.7

36.1

1.10380372

1.5575072

1.719182245

1.21838265

6

12.3

32.3

1.08990511

1.50920252

1.644887543

1.18789315

7

11.9

27.5

1.07554696

1.43933269

1.548069905

1.15680127

8

13.7

48.1

1.13672057

1.68214508

1.912128905

1.29213365

9

13

36.5

1.11394335

1.56229286

1.740305751

1.24086979

8.857619

10.70627

11.40692

9.002839

total

Log W

Log L2

b. Ikan Hampala (Hampala macrolepidota)
No

Length (L)

Weight (W)

Log L

Log W

1
2
3

11.2
12.2
11.4

5.7
6.1
5.8

1.04921802
1.08635983
1.05690485

0.75587486
0.78532984
0.76342799

0.793077521
0.853150787
0.80687075

1.10085846
1.18017768
1.11704786

3.192483

2.304633

2.453099

4.562072

Total

Log L*Log W

Log L2

c. Ikan cencen (Mystacoleucus marginatus)
No

Length (L)
12.6

Weight
(W)
18.8

Log L

Log W

Log L*Log W

Log L2

1.10037055

1.27415785

1.402045767

1.21081534

12.7

20.7

1.10380372

1.31597035

1.452572964

1.21838265

9.6

7.3

0.98227123

0.86332286

0.84801721

0.96485678

9.2

6.9

0.96378783

0.83884909

0.808472543

0.92888698

7.5

4.5

0.87506126

0.65321251

0.571600968

0.76573221

Total

5.02529459

4.94551266

5.082709452

5.08867396

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d. Ikan (Puntius lateristriga)
No

Length (L)

Weight (W)

Log L

1

10.8

8.5

1.03342376

0.92941893

0.960483597

1.06796466

2

11.4

8.9

1.05690485

0.94939001

1.003414904

1.11704786

2.090329

1.858838

1.942791

2.185013

Total

Log W

Log L*Log W

Log L2

e. Ikan jurung (Tor tambroides)

No

Length (L)

Weight (W)

Log L

Log W
0.91907809

Log L*Log
W
0.906920283

1

9.7

8.3

0.98677173

2

9.1

7.8

3

14

4

0.97371846

0.95904139

0.8920946

0.85555565

0.91976039

25.6

1.14612804

1.40823997

1.614023305

1.31360947

11.5

13.1

1.06069784

1.1172713

1.18508725

1.12507991

5

11.6

14.4

1.06445799

1.15836249

1.233028209

1.13307081

6

12.6

14.2

1.10037055

1.15228834

1.267944154

1.21081534

6.317468

6.647335

7.062559

6.676054

Total

Log L2

f. Ikan Baung (Mystus nemurus)
No

Length (L)

Weight (W)

Log L

Log W
0.90848502

Log L*Log
W
0.92773518

1

10.5

8.1

1.0211893

2

12.8

9.7

3

11.7

4

Log L2
1.04282758

1.10720997

0.98677173

1.092563502

1.22591392

9.1

1.06818586

0.95904139

1.024434456

1.14102104

13.3

9.5

1.12385164

0.97772361

1.098816278

1.26304251

5

12.2

9.4

1.08635983

0.97312785

1.05716701

1.18017768

6

11.8

9.4

1.07188201

0.97312785

1.043078237

1.14893104

7

12

9.3

1.07918125

0.96848295

1.045168635

1.16463216

8

11.9

9.5

1.07554696

0.97772361

1.051587653

1.15680127

Total

8.633407

7.724484

8.340551

9.323347

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g.

Ikan Lele Merah (Glypthotorax platygonoides)

No

Length (L)

Weight (W)

Log L

Log W
0.77085201

Log L*Log
W
0.876243336

1

13.7

5.9

1.13672057

2

14.2

6.1

3

14.3

4

1.29213365

1.15228834

0.78532984

0.904926415

1.32776843

6.2

1.15533604

0.79239169

0.915478675

1.33480136

13.7

6

1.13672057

0.77815125

0.884540531

1.29213365

5

13.5

5.7

1.13033377

0.75587486

0.854390874

1.27765443

6

14.1

6.3

1.14921911

0.79934055

0.918617437

1.32070457

7

13.2

5.1

1.12057393

0.70757018

0.792884694

1.25568594

8

13.8

6

1.13987909

0.77815125

0.886998336

1.29932433

Total

9.121071

6.167662

7.03408

10.40021

Log a =

Log b =

Log L2

∑ log W x ∑(log L)2− ∑ log L x ∑(log L x log W )
N.∑(log L)2−(∑ log L)2

∑ log W −(N log a)
∑ log L

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Lampiran 8. Contoh Perhitungan
Kepadatan (K) ikan Oreochromis niloticus
Jumlah individu suatu spesies / ulangan
Luas jala

KP =

12/30

=

12,56

jumlah K dalam setiap spesies
x 100 %
total K

KR =
=

FK =

=

= 0,013 ind/m2

0,013
0,034

x100% = 38,46%

Jumlah plot yang ditempati suatu jenis
x 100%
Jumlah total plot
5
30

x 100% = 16,67%

Indeks Diversitas Shannon-Wiener (Indeks Keanekaragaman) stasiun 2
H’ = − ∑ pi ln pi
H’ = - ∑ (� ln �+� ln �+ � ln �+� ln �
5

5

2

2

2

2

4

4

13

13

13

13

13

13

13

13

H’ = - ∑ (- 0,363)+(- 0,28)+(- 0,28)+ (- 0, 353)
H’= 1,306
Indeks Keseragaman
H'
E=
H max
1,306
E =
ln 4
= 0,942
Indeks Similaritas
2c
x100%
IS =
a+b
2( 2)
x100%
IS =
4+4
= 50%

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Lampiran 9. Hasil Korelasi
Correlations
Keanekaragaman
Keanekaragaman

Pearson Correlation

1

Sig. (2-tailed)
N
Suhu

Pearson Correlation
Sig. (2-tailed)
N

Kec. Arus

Kej. Oksigen

.611

.097

.690

.056

.156

.083

3

3

3

3

3

3

1

.611

pH
BOD Nitrat Phosfat
.574 -.719 -.574
-.981
.611

.490

3

3

**

-.463 -1.000

.611

.123

3

3

**

.982 1.000

.721

-.691

.455

-.500

-.756

.514

.699

.667

.454

.693

.000

.121

.000

.488

3

3

3

3

-.691

-.999*

.514

.026

3

3

3

3

3

Pearson Correlation

.988

-.691

1

.329

.971

.996

.960

.691 -.816

Sig. (2-tailed)

.097

.514

.787

.153

.060

.180

.514

.393

3

3

3

3

3

3

3

3

3

3

3

Pearson Correlation

.468

.455

.329

1

.544

.239

.579

-.455

.278

.455

-.290

Sig. (2-tailed)

.690

.699

.787

.634

.846

.607

.699

.820

.699

.813

3

3

3

3

3

3

3

3

3

3

.945

*

.500 -.655

-.500

-.961

3

Pearson Correlation

.996

-.500

.971

.544

Sig. (2-tailed)

.056

.667

.153

.634

.212

.027

.667

.546

.667

.179

3

3

3

3

3

3

3

3

3

3

3

Pearson Correlation

.970

-.756

.996

.239

.945

1

.930

.756 -.866

-.756

-.999*

Sig. (2-tailed)
N

.156
3

.454
3

.060
3

.846
3

.212
3

3

.239
3

.454
3

.333
3

.454
3

.033
3

.992

-.463

.960

1

.463 -.622

-.463

-.948

N
DO

Kej.
DO
Oksigen
.970
.992

3

N
Pen. Cahaya

-.574

Pen.
Cahaya
.996

3

N
Int. Cahaya

3

Suhu
Kec. Arus Int. Cahaya
-.574
.988
.468

Pearson Correlation

1

.999

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Sig. (2-tailed)

.083

.693

.180

.607

.027

.239

3

3

3

3

3

3

Pearson Correlation

.574

**

.691

-.455

.500

.756

.463

Sig. (2-tailed)

.611

.000

.514

.699

.667

.454

.693

3

3

3

3

3

3

3

-.719

.982

-.816

.278

-.655

-.866

.490

.121

.393

.820

.546

3

3

3

3

3

-.574

**

-.691

.455

-.500

-.756

.611

.000

.514

.699

.667

.454

.693

.000

3

3

3

3

3

3

3

3

3

3

3

-.981

.721

*

-.290

-.961

*

-.948

-.721

.839

.721

1

.123

.488

.026

.813

.179

.033

.206

.488

.367

.488

3

3

3

3

3

3

3

3

3

3

N
pH

N

BOD

Pearson Correlation
Sig. (2-tailed)
N

Nitrat

Pearson Correlation
Sig. (2-tailed)
N

Phosfat

Pearson Correlation
Sig. (2-tailed)
N

-1.000

1.000

-.999

.693

.572

3

3

.206

3

3

**

-.721

.121

.000

.488

3

3

3

3

-.622

-.982

1

.982

.839

.333

.572

.121

.121

.367

3

3

3

3

3

3

**

.982

1

.721

-.999

3

.693

1 -.982 -1.000

-.463 -1.000

.488

3

**. Correlation is significant at the 0.01 level (2-tailed).
*. Correlation is significant at the 0.05 level (2-tailed).

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