Analisis Logam Berat (Pb dan Cd) yang Terakumulasi pada Eceng Gondok (Eichornia Crassipes Solms.) di Sungai Asahan, Kabupaten Toba Samosir

LAMPIRAN

A. Peta Lokasi

Universitas Sumatera Utara

B. Bagan Kerja DO (Desolved Oxygen)

Sampel Air
1 ml MnSO4
1 ml KOHKI
Dikocok
Didiamkan
Sampel Endapan
Puith/Cokelat
1 ml H2SO4
Dikocok
Didiamkan
Larutan Sampel
Berwarna Cokelat
Diambil 100 ml

Dititrasi Na2S2O3 0,00125 N
Sampel Berwarna
Kuning Pucat
Ditambah 5 tetes Amilum
Sampel
Berwarna Biru
Dititrasi dengan Na2S2O3
0,00125 N
Sampel Bening
Dihitung volume
Na2S2O3yang terpakai
Hasil

Universitas Sumatera Utara

C. Bagan Kerja BOD5 (Biochemical Oxygen Demand)

Universitas Sumatera Utara

D. Bagan Kerja Kandungan Nitrat (NO3-)

5 ml samel air

1 ml NaCl (dengan pipet volume)
5 ml H2SO4 75 %
4 tetes Brucine sulfat sulfanic acid
Larutan

Dipanaskan selama 25 menit, suhu 950C

Larutan
Didinginkan
Diukur dengan spektrofotometer pada λ= 410
nm
Hasil (konsentrasi
Nitrrat)

Universitas Sumatera Utara

E. Bagan kerja Analisis Fosfat (PO4-)

5 ml samel air

2 ml Amstrong reagen
1 ml Asorbic Acid

Larutan

Dibiarkan selama 25 menit
Diukur dengan spektrofotometer pada λ= 880
nm
Hasil (konsentrasi
Fospat)

Universitas Sumatera Utara

F. Contoh Perhitungan

a. Konsentrasi Sebenarnya

K sebenarnya (mg/kg)=

�
,

=

�

��

.

�

�

� �

= 0,0082 mg/l

b. Biokonsentrasi Faktor (BCF)
BCF Pb/Cd

�

=

=

,
,

� /

�

� /

��

ℎ

8

= 455,62

Universitas Sumatera Utara

G. Hasil Analisis Varians ver 13.00

Tests of Normality
a

LOGAM_Cd

VAR00001
AIR
DAUN
BATANG
AKAR

SEDIMEN

Kolmogorov-Smirnov
Statistic
df
Sig.
.349
3
.360
3
.318
3
.175
3
.312
3

.
.
.

.
.

Statistic
.832
.808
.887
1.000
.896

Shapiro-Wilk
df
3
3
3
3
3

Sig.
.194

.133
.346
.994
.374

a. Lilliefors Signific ance Correction
Multiple Comparisons
Dependent Variable: LOGAM_Cd
Tukey HSD

(I) VAR00001
AIR

DAUN

BATANG

AKAR

SEDIMEN

(J) VAR00001
DAUN
BATANG
AKAR
SEDIMEN
AIR
BATANG
AKAR
SEDIMEN
AIR
DAUN
AKAR
SEDIMEN
AIR
DAUN
BATANG
SEDIMEN
AIR
DAUN

BATANG
AKAR

Mean
Difference
Std. Error
(I-J)
-.3321333* .0094521
-.8677333* .0094521
-.8514000* .0094521
-1.3937000* .0094521
.3321333* .0094521
-.5356000* .0094521
-.5192667* .0094521
-1.0615667* .0094521
.8677333* .0094521
.5356000* .0094521
.0163333
.0094521
-.5259667* .0094521
.8514000* .0094521
.5192667* .0094521
-.0163333
.0094521
-.5423000* .0094521
1.3937000* .0094521
1.0615667* .0094521
.5259667* .0094521
.5423000* .0094521

Sig.
.000
.000
.000
.000
.000
.000
.000
.000
.000
.000
.460
.000
.000
.000
.460
.000
.000
.000
.000
.000

95% Confidence Interval
Lower Bound Upper Bound
-.363241
-.301026
-.898841
-.836626
-.882508
-.820292
-1.424808
-1.362592
.301026
.363241
-.566708
-.504492
-.550374
-.488159
-1.092674
-1.030459
.836626
.898841
.504492
.566708
-.014774
.047441
-.557074
-.494859
.820292
.882508
.488159
.550374
-.047441
.014774
-.573408
-.511192
1.362592
1.424808
1.030459
1.092674
.494859
.557074
.511192
.573408

*. The mean difference is significant at the .05 level.

Universitas Sumatera Utara

Tests of Normality
a

LOGAM_Pb

VAR00001
AIR
DAUN
BATANG
AKAR
SEDIMEN

Kolmogorov-Smirnov
Statistic
df
Sig.
.253
3
.326
3
.181
3
.228
3
.286
3

.
.
.
.
.

Statistic
.964
.874
.999
.982
.930

Shapiro-Wilk
df
3
3
3
3
3

Sig.
.637
.308
.939
.743
.490

a. Lilliefors Signific ance Correction

Multiple Comparisons
Dependent Variable: LOGAM_Pb
Tukey HSD

(I) VAR00001
AIR

DAUN

BATANG

AKAR

SEDIMEN

(J) VAR00001
DAUN
BATANG
AKAR
SEDIMEN
AIR
BATANG
AKAR
SEDIMEN
AIR
DAUN
AKAR
SEDIMEN
AIR
DAUN
BATANG
SEDIMEN
AIR
DAUN
BATANG
AKAR

Mean
Difference
(I-J)
Std. Error
-1.2109667* .0594887
-1.9416000* .0594887
-2.5640333* .0594887
-3.1688667* .0594887
1.2109667* .0594887
-.7306333* .0594887
-1.3530667* .0594887
-1.9579000* .0594887
1.9416000* .0594887
.7306333* .0594887
-.6224333* .0594887
-1.2272667* .0594887
2.5640333* .0594887
1.3530667* .0594887
.6224333* .0594887
-.6048333* .0594887
3.1688667* .0594887
1.9579000* .0594887
1.2272667* .0594887
.6048333* .0594887

Sig.
.000
.000
.000
.000
.000
.000
.000
.000
.000
.000
.000
.000
.000
.000
.000
.000
.000
.000
.000
.000

95% Confidence Interval
Lower Bound Upper Bound
-1.406749
-1.015185
-2.137382
-1.745818
-2.759815
-2.368251
-3.364649
-2.973085
1.015185
1.406749
-.926415
-.534851
-1.548849
-1.157285
-2.153682
-1.762118
1.745818
2.137382
.534851
.926415
-.818215
-.426651
-1.423049
-1.031485
2.368251
2.759815
1.157285
1.548849
.426651
.818215
-.800615
-.409051
2.973085
3.364649
1.762118
2.153682
1.031485
1.423049
.409051
.800615

*. The mean difference is significant at the .05 level.

Universitas Sumatera Utara