Pengaruh Penyinaran Sinar Ultraviolet C (UV-C) Sebagai Elisitor Untuk Meningkatkan Produksi Katekin Melalui Kultur Kalus Pucuk Daun Teh (Camellia sinensis L.)
LAMPIRAN
Lampiran 1. Gambar Penelitian
a. Tanaman teh yang digunakan sebagai sumber eksplan
b. Hasil ektraksi kalus teh (Camellia sinensis L.)
Universitas Sumatera Utara
32
Lampiran 2 . Komposisi Media MS (Murashige & Skoog) 1962
Bahan Kimia
Makro Nutrien
NH4NO3
KNO3
CaCl2 . H2O
MgSO4 . 7H2O
KH2PO4
Iron
Na2EDTA
FeSO4 . 7H2O
Mikro Nutrien
MnSO4 . 4H2O
ZnSO4 . 7H2O
H3BO3
KI
NaMoO4 . 2H2O
CuSO4 . 5H2O
CoCl . 6H2O
Vitamin
Glycine
Nicotine Acid
Pyrodoxin HCl
Thyamine HCl
Myo-inositol
pH
Gula
Agar
Konsentrasi Dalam Media (mg/l)
1650,000
1900,000
440,000
370,000
170,000
37,000
27,000
22,300
8,600
6,200
0,830
0,250
0,025
0,025
2,000
0,500
0,500
0,100
100,000
5,8
3g
7g
Universitas Sumatera Utara
33
Lampiran 3. Data Berat Kering Kalus (g) dan Uji Statistik
Perlakuan
K0
K1
K2
K3
U1
0,21
0,17
0,24
0,19
U2
0,26
0,17
0,23
0,26
U3
0,33
0,20
0,25
0,17
U4
0,21
0,17
0,24
0,19
U5
0,26
0,20
0,25
0,17
U6
0,30
0,19
0,23
0,26
Jumlah Rata-rata
1,57
0,26
1,10
0,18
1,44
0,24
1,24
0,20
Uji Statistik
Descriptives
Ulangan
N
K0
K1
K2
K3
Total
Mean
6
6
6
6
24
,2617
,1833
,2400
,2067
,2229
Std.
Deviation
,04792
,01506
,00894
,04227
,04359
Test of Homogeneity of Variances
Ulangan
Levene
df1
df2
Statistic
4,960
3
20
Std.
Error
,01956
,00615
,00365
,01726
,00890
Sig.
,010
ANOVA
Ulangan
Between
Groups
Within Groups
Total
Sum of
Squares
,022
,022
,044
df
3
20
23
Mean
Square
,007
F
6,605
Sig.
,003
,001
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34
Ulangan
Duncana
Perlakuan_bb
N
Subset for alpha = 0.05
s
1
2
3
K1
6
,1833
,2067
K3
6
,2067
,2400
K2
6
,2400
K0
6
,2617
,237
,097
Sig.
,271
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 6,000.
Universitas Sumatera Utara
35
Lampiran 4. Indeks Pertumbuhan (g) dan Uji Statistik
Perlakuan
K0
K1
K2
K3
U1
0,13
0,12
0,13
0,13
U2
0,16
0,12
0,12
0,16
U3
0,18
0,13
0,14
0,13
U4
0,15
0,09
0,17
0,12
U5
0,18
0,10
0,16
0,10
U6
0,21
0,10
0,17
0,14
Jumlah Rata-rata
1,01
0,16
0,66
0,11
0,89
0,15
0,78
0,13
Uji Statistik
Descriptives
Ulangan
N
K0
K1
K2
K3
Total
Mean
6
6
6
6
24
,1683
,1100
,1483
,1300
,1392
Std.
Deviation
,02787
,01549
,02137
,02000
,02992
Test of Homogeneity of Variances
Ulangan
Levene
df1
df2
Statistic
,833
3
20
Std.
Error
,01138
,00632
,00872
,00816
,00611
Sig.
,491
ANOVA
Ulangan
Between
Groups
Within Groups
Total
Sum of
Squares
,011
,009
,021
df
3
20
23
Mean
Square
,004
F
7,983
Sig.
,001
,000
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36
Ulangan
Duncana
Perlakuan_bb
N
Subset for alpha = 0.05
s
1
2
3
K1
6
,1100
,1300
K3
6
,1300
,1483
K2
6
,1483
K0
6
,1683
,125
,158
Sig.
,125
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 6,000.
Universitas Sumatera Utara
37
Lampiran 5. Luas Area Standar, Retention Time
Linear
dan Uji Statistik Regresi
Konsentrasi ( ppm )
Rata-Rata Luas Area
Rata-Rata Retention
Time
250
500
750
1000
1210266,5
2142538,5
3643158
6249108,5
3,3025
3,3125
3,3134
3,3015
Rata-rata
3,3074
Uji Statistik
SUMMARY OUTPUT
Regression Statistics
0,97506
Multiple R
0,950742
R Square
Adjusted R
Square
0,926114
598077,7
Standard Error
4
Observations
ANOVA
df
Regression
Residual
Total
Intercept
X Variable 1
1
2
3
SS
MS
F
1,38E+13 1,38E+13 38,60289
7,15E+11 3,58E+11
1,45E+13
Significance
F
0,02494
Standard
Coefficients Error
t Stat
P-value
-843519
732492,6 -1,15157 0,368574
6647,258
1069,874 6,213123 0,02494
Universitas Sumatera Utara
38
Lampiran 6. Kurva Analisis Regresi Linear Standar Katekin
7000000
y = 8215,14x - 2146753
R²= 0,97729
6000000
Luas Area
5000000
4000000
3000000
2000000
1000000
0
0
200
400
600
800
Konsentrasi ( ppm )
1000
1200
Universitas Sumatera Utara
39
Lampiran 7. Luas Area Perlakuan dan Retention Time
Perlakuan
Rata-Rata Luas Area
K0
K1
K2
K3
536592
1168564
5913723
874057
Rata-Rata Retention Time
Rata-Rata
3,3905
3,3855
3,5405
3,3910
3,342687
Universitas Sumatera Utara
40
Lampiran 8. Retention Time Standar dan Sampel
Standar (ppm)
250
500
750
1000
Retention time
Ulangan 1
Ulangan 2
3,3010
3,3090
3,3080
3,3300
3,3040
3,3160
3,3180
3,3200
K0
K1
K2
K3
Rata-Rata
3,3025
3,3125
3,3130
3,2800
3,3020
Rata-Rata
Sampel
Rata- Rata
Retention time
Ulangan 1
Ulangan 2
3,3840
3,3890
3,5410
3,3920
3,3970
3,3820
3,5400
3,3920
Rata- Rata
3,3905
3,3855
3,5405
3,3920
3,4271
Universitas Sumatera Utara
41
Lampiran 9. Analisis Kandungan Katekin Secara Kuantitatif dan Uji Statistik
Perlakuan
Rata-Rata Luas Area Sampel
Kadar Katekin
(ppm)
K0
K1
K2
K3
536592
1168564
5913723
874057
326,63412
402,09348
981,17330
367,71254
Universitas Sumatera Utara
42
Uji Statistik
Descriptives
Ulangan
Statistic
Kontrol
N
Mean
Std. Deviation
Std. Error
95% Confidence Interval for Mean
UV C 15
Minimum
Maximum
N
Mean
Std. Deviation
Std. Error
95% Confidence Interval for Mean
UV C 30
Lower Bound
Upper Bound
Minimum
Maximum
N
Mean
Std. Deviation
Std. Error
95% Confidence Interval for Mean
Total
Lower Bound
Upper Bound
Minimum
Maximum
N
Mean
Std. Deviation
Std. Error
95% Confidence Interval for Mean
UV C 45
Lower Bound
Upper Bound
Lower Bound
Upper Bound
Minimum
Maximum
N
Mean
Std. Deviation
Std. Error
95% Confidence Interval for Mean
Minimum
Maximum
Lower Bound
Upper Bound
2
619697,0000
117528,21810
83105,00000
-436252,1446
1675646,1446
536592,00
702802,00
2
1137573,5000
43827,18540
30990,50000
743801,8621
1531345,1379
1,11E+006
1,17E+006
2
5817846,5000
135589,84661
95876,50000
4599620,0616
7036072,9384
5,72E+006
5,91E+006
2
957563,0000
118095,31774
83506,00000
-103481,3327
2018607,3327
874057,00
1,04E+006
8
2133170,0000
2284402,89467
807658,38889
223361,3866
4042978,6134
536592,00
5,91E+006
Universitas Sumatera Utara
43
Test of Homogeneity of Variances
Ulangan
Levene
df1
df2
Statistic
116511859656
3
4
4764,000
Sig.
,000
ANOVA
Ulangan
Between Groups
Within Groups
Total
Ulangan
Duncana
Perlakuan
N
Sum of
Squares
36481411281
285,000
48064814807,
000
36529476096
092,000
df
Mean Square
3 121604704270
95,000
4 12016203701,
750
7
F
1012,006
Sig.
,000
Subset for alpha = 0.05
1
2
3
619697,0000
957563,0000
1137573,5000 5817846,5000
Kontrol
2
UV C 45
2
UV C 15
2
UV C 30
2
1,000
,176
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 2,000.
1,000
Universitas Sumatera Utara
44
Lampiran 10. Out Put High Performance Liquid Chromatography ( HPLC )
a. Out put Standar Katekin 250 Ppm
b. Out put Ektrak Katekin Disinari UV C 30 menit
Universitas Sumatera Utara
45
Lampiran 11. Perhitungan Kandungan Katekin Sampel Secara Kuantitatif
Y = bx + a
Y = 8215,14 x - 2146753
Keterangan:
Y = Luas Area Sampel
b = koefisien regresi
a = konstansta luas area standar
x = kadar katekin
Kandungan katekin kontrol
Y = bx + a
536592
= 8215,14 x – 2146753
-8215,14 x = -536592 – 2146753
x = 326,63412 ppm
Universitas Sumatera Utara
Lampiran 1. Gambar Penelitian
a. Tanaman teh yang digunakan sebagai sumber eksplan
b. Hasil ektraksi kalus teh (Camellia sinensis L.)
Universitas Sumatera Utara
32
Lampiran 2 . Komposisi Media MS (Murashige & Skoog) 1962
Bahan Kimia
Makro Nutrien
NH4NO3
KNO3
CaCl2 . H2O
MgSO4 . 7H2O
KH2PO4
Iron
Na2EDTA
FeSO4 . 7H2O
Mikro Nutrien
MnSO4 . 4H2O
ZnSO4 . 7H2O
H3BO3
KI
NaMoO4 . 2H2O
CuSO4 . 5H2O
CoCl . 6H2O
Vitamin
Glycine
Nicotine Acid
Pyrodoxin HCl
Thyamine HCl
Myo-inositol
pH
Gula
Agar
Konsentrasi Dalam Media (mg/l)
1650,000
1900,000
440,000
370,000
170,000
37,000
27,000
22,300
8,600
6,200
0,830
0,250
0,025
0,025
2,000
0,500
0,500
0,100
100,000
5,8
3g
7g
Universitas Sumatera Utara
33
Lampiran 3. Data Berat Kering Kalus (g) dan Uji Statistik
Perlakuan
K0
K1
K2
K3
U1
0,21
0,17
0,24
0,19
U2
0,26
0,17
0,23
0,26
U3
0,33
0,20
0,25
0,17
U4
0,21
0,17
0,24
0,19
U5
0,26
0,20
0,25
0,17
U6
0,30
0,19
0,23
0,26
Jumlah Rata-rata
1,57
0,26
1,10
0,18
1,44
0,24
1,24
0,20
Uji Statistik
Descriptives
Ulangan
N
K0
K1
K2
K3
Total
Mean
6
6
6
6
24
,2617
,1833
,2400
,2067
,2229
Std.
Deviation
,04792
,01506
,00894
,04227
,04359
Test of Homogeneity of Variances
Ulangan
Levene
df1
df2
Statistic
4,960
3
20
Std.
Error
,01956
,00615
,00365
,01726
,00890
Sig.
,010
ANOVA
Ulangan
Between
Groups
Within Groups
Total
Sum of
Squares
,022
,022
,044
df
3
20
23
Mean
Square
,007
F
6,605
Sig.
,003
,001
Universitas Sumatera Utara
34
Ulangan
Duncana
Perlakuan_bb
N
Subset for alpha = 0.05
s
1
2
3
K1
6
,1833
,2067
K3
6
,2067
,2400
K2
6
,2400
K0
6
,2617
,237
,097
Sig.
,271
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 6,000.
Universitas Sumatera Utara
35
Lampiran 4. Indeks Pertumbuhan (g) dan Uji Statistik
Perlakuan
K0
K1
K2
K3
U1
0,13
0,12
0,13
0,13
U2
0,16
0,12
0,12
0,16
U3
0,18
0,13
0,14
0,13
U4
0,15
0,09
0,17
0,12
U5
0,18
0,10
0,16
0,10
U6
0,21
0,10
0,17
0,14
Jumlah Rata-rata
1,01
0,16
0,66
0,11
0,89
0,15
0,78
0,13
Uji Statistik
Descriptives
Ulangan
N
K0
K1
K2
K3
Total
Mean
6
6
6
6
24
,1683
,1100
,1483
,1300
,1392
Std.
Deviation
,02787
,01549
,02137
,02000
,02992
Test of Homogeneity of Variances
Ulangan
Levene
df1
df2
Statistic
,833
3
20
Std.
Error
,01138
,00632
,00872
,00816
,00611
Sig.
,491
ANOVA
Ulangan
Between
Groups
Within Groups
Total
Sum of
Squares
,011
,009
,021
df
3
20
23
Mean
Square
,004
F
7,983
Sig.
,001
,000
Universitas Sumatera Utara
36
Ulangan
Duncana
Perlakuan_bb
N
Subset for alpha = 0.05
s
1
2
3
K1
6
,1100
,1300
K3
6
,1300
,1483
K2
6
,1483
K0
6
,1683
,125
,158
Sig.
,125
Means for groups in homogeneous subsets are
displayed.
a. Uses Harmonic Mean Sample Size = 6,000.
Universitas Sumatera Utara
37
Lampiran 5. Luas Area Standar, Retention Time
Linear
dan Uji Statistik Regresi
Konsentrasi ( ppm )
Rata-Rata Luas Area
Rata-Rata Retention
Time
250
500
750
1000
1210266,5
2142538,5
3643158
6249108,5
3,3025
3,3125
3,3134
3,3015
Rata-rata
3,3074
Uji Statistik
SUMMARY OUTPUT
Regression Statistics
0,97506
Multiple R
0,950742
R Square
Adjusted R
Square
0,926114
598077,7
Standard Error
4
Observations
ANOVA
df
Regression
Residual
Total
Intercept
X Variable 1
1
2
3
SS
MS
F
1,38E+13 1,38E+13 38,60289
7,15E+11 3,58E+11
1,45E+13
Significance
F
0,02494
Standard
Coefficients Error
t Stat
P-value
-843519
732492,6 -1,15157 0,368574
6647,258
1069,874 6,213123 0,02494
Universitas Sumatera Utara
38
Lampiran 6. Kurva Analisis Regresi Linear Standar Katekin
7000000
y = 8215,14x - 2146753
R²= 0,97729
6000000
Luas Area
5000000
4000000
3000000
2000000
1000000
0
0
200
400
600
800
Konsentrasi ( ppm )
1000
1200
Universitas Sumatera Utara
39
Lampiran 7. Luas Area Perlakuan dan Retention Time
Perlakuan
Rata-Rata Luas Area
K0
K1
K2
K3
536592
1168564
5913723
874057
Rata-Rata Retention Time
Rata-Rata
3,3905
3,3855
3,5405
3,3910
3,342687
Universitas Sumatera Utara
40
Lampiran 8. Retention Time Standar dan Sampel
Standar (ppm)
250
500
750
1000
Retention time
Ulangan 1
Ulangan 2
3,3010
3,3090
3,3080
3,3300
3,3040
3,3160
3,3180
3,3200
K0
K1
K2
K3
Rata-Rata
3,3025
3,3125
3,3130
3,2800
3,3020
Rata-Rata
Sampel
Rata- Rata
Retention time
Ulangan 1
Ulangan 2
3,3840
3,3890
3,5410
3,3920
3,3970
3,3820
3,5400
3,3920
Rata- Rata
3,3905
3,3855
3,5405
3,3920
3,4271
Universitas Sumatera Utara
41
Lampiran 9. Analisis Kandungan Katekin Secara Kuantitatif dan Uji Statistik
Perlakuan
Rata-Rata Luas Area Sampel
Kadar Katekin
(ppm)
K0
K1
K2
K3
536592
1168564
5913723
874057
326,63412
402,09348
981,17330
367,71254
Universitas Sumatera Utara
42
Uji Statistik
Descriptives
Ulangan
Statistic
Kontrol
N
Mean
Std. Deviation
Std. Error
95% Confidence Interval for Mean
UV C 15
Minimum
Maximum
N
Mean
Std. Deviation
Std. Error
95% Confidence Interval for Mean
UV C 30
Lower Bound
Upper Bound
Minimum
Maximum
N
Mean
Std. Deviation
Std. Error
95% Confidence Interval for Mean
Total
Lower Bound
Upper Bound
Minimum
Maximum
N
Mean
Std. Deviation
Std. Error
95% Confidence Interval for Mean
UV C 45
Lower Bound
Upper Bound
Lower Bound
Upper Bound
Minimum
Maximum
N
Mean
Std. Deviation
Std. Error
95% Confidence Interval for Mean
Minimum
Maximum
Lower Bound
Upper Bound
2
619697,0000
117528,21810
83105,00000
-436252,1446
1675646,1446
536592,00
702802,00
2
1137573,5000
43827,18540
30990,50000
743801,8621
1531345,1379
1,11E+006
1,17E+006
2
5817846,5000
135589,84661
95876,50000
4599620,0616
7036072,9384
5,72E+006
5,91E+006
2
957563,0000
118095,31774
83506,00000
-103481,3327
2018607,3327
874057,00
1,04E+006
8
2133170,0000
2284402,89467
807658,38889
223361,3866
4042978,6134
536592,00
5,91E+006
Universitas Sumatera Utara
43
Test of Homogeneity of Variances
Ulangan
Levene
df1
df2
Statistic
116511859656
3
4
4764,000
Sig.
,000
ANOVA
Ulangan
Between Groups
Within Groups
Total
Ulangan
Duncana
Perlakuan
N
Sum of
Squares
36481411281
285,000
48064814807,
000
36529476096
092,000
df
Mean Square
3 121604704270
95,000
4 12016203701,
750
7
F
1012,006
Sig.
,000
Subset for alpha = 0.05
1
2
3
619697,0000
957563,0000
1137573,5000 5817846,5000
Kontrol
2
UV C 45
2
UV C 15
2
UV C 30
2
1,000
,176
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 2,000.
1,000
Universitas Sumatera Utara
44
Lampiran 10. Out Put High Performance Liquid Chromatography ( HPLC )
a. Out put Standar Katekin 250 Ppm
b. Out put Ektrak Katekin Disinari UV C 30 menit
Universitas Sumatera Utara
45
Lampiran 11. Perhitungan Kandungan Katekin Sampel Secara Kuantitatif
Y = bx + a
Y = 8215,14 x - 2146753
Keterangan:
Y = Luas Area Sampel
b = koefisien regresi
a = konstansta luas area standar
x = kadar katekin
Kandungan katekin kontrol
Y = bx + a
536592
= 8215,14 x – 2146753
-8215,14 x = -536592 – 2146753
x = 326,63412 ppm
Universitas Sumatera Utara