Pengaruh Penambahan Minyak Biji Anggur (Grape Seed Oil) Terhadap Efektivitas Sediaan Tabir Surya Kombinasi Oksibenzon dan Oktilmetoksisinamat Dalam Basis Vanishing Cream

PENGARUH PENAMBAHAN MINYAK BIJI ANGGUR (Grape Seed Oil)
TERHADAP EFEKTIVITAS SEDIAAN TABIR SURYA
KOMBINASI OKSIBENZON DAN OKTILMETOKSINAMAT
DALAM BASIS VANISHING CREAM

ABSTRAK
Latar belakang: Minyak biji anggur/ grape seed oil (MBA) merupakan salah
satu minyak nabati yang sering digunakan dalam industri kosmetik, kuliner, serta
untuk tujuan farmasetis maupun medis. Minyak biji anggur mengandung asam
lemak tidak jenuh berupa asam linoleat dan asam linolenat dalam konsentrasi
tinggi. Minyak biji anggur juga mengandung senyawa fenolik, tokoferol, serta
tannin yang dapat melindungi kulit dari bahaya radiasi ultraviolet matahari.
Tujuan dari penelitian ini adalah untuk melihat pengaruh penambahan minyak
biji anggur terhadap peningkatan efektivitas sediaan tabir surya kombinasi
oksibenzon dan oktilmetoksisinamat (2 dan 5 % b/b) dengan parameter nilai SPF
(Sun Protection Factor).
Metode: Sediaan dibuat dalam 5 formula yaitu formula A (blanko), formula B
(6% MBA), formula C (8% MBA), formula D (10% MBA), formula E (12%
MBA) yang dalam formulanya masing-masing mengandung oksibenzon dan
oktilmetoksisinamat (2 dan 5 % b/b). Evaluasi sediaan krim dilakukan terhadap
homogenitas, tipe emulsi, pH, stabilitas (warna dan bau), iritasi kulit, dan

efektivitas SPF sediaan secara in vitro menggunakan spektrofotometer UV.
Selanjutnya nilai SPF yang diperoleh diuji dengan One Way Anova untuk
mengetahui apakah terdapat perbedaan yang signifikan diantara kelima formula.
Hasil: Hasil pengujian menunjukkan seluruh sampel homogen dengan tipe emulsi
m/a, rentang pH pada kisaran 6,53 - 6,56, tidak menyebabkan iritasi dan sediaan
stabil selama penyimpanan 12 minggu pada suhu kamar. Hasil penelitian juga
menunjukkan bahwa nilai SPF sediaan, meningkat seiring dengan meningkatnya
konsentrasi minyak biji anggur yang ditambahkan dengan nilai SPF rata-rata dari
masing-masing formula sebagai berikut: formula A 11,0714; formula B 11,9054;
formula C 12,3189; formula D 12,7222; serta formula E 13,0343. Kelima formula
tersebut dapat digunakan secara optimal sebagai tabir surya dengan kategori
proteksi maksimal. Dari hasil uji statistik diketahui bahwa terdapat perbedaan
yang
signifikan
terhadap
nilai
SPF
kelima
formula
sediaan.

Kesimpulan: Dari penelitian yang dilakukan dapat disimpulkan bahwa dengan
penambahan minyak biji anggur dapat meningkatkan efektivitas sediaan tabir
surya dari oksibenzon dan oktilmetoksisinamat dalam basis vanishing cream.
Kata kunci: Tabir surya, minyak biji anggur, oksibenzon, oktilmetoksisinamat,
SPF (Sun Protection Factor)

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THE INFLUENCE OF GRAPE SEED OIL AGAINST THE
EFFECTIVENESS OF SUNSCREEN PREPARATIONS CONTAINING
THE COMBINATION OF OXYBENZONE AND
OCTILMETOXYCINNAMATE IN VANISHING CREAM BASIS

ABSTRACT
Background: Grape seed oil is a vegetable oil that is usually used i n cosmetic
industry, culinary, pharmaceutic and also medical used. Grape seed oil contains of
unsaturated fatty acid such as linoleic acid and linolenic acid in high concentration.
Grape seed oil also contain phenolic compound, tocoferol and tannin which can
protect skin from the impact of sun’s ultraviolet radiation.
Purpose: The aim of this study was to evaluate the influence of adding grape seed

oil to increase the effectiveness of sunscreen preparations containing the
combination of oxybenzone and octylmetoxycinnamate (2 and 5 % b/b) by
measuring the SPF (Sun Protection Factor) value as its parameter.
Methods: In this study, the preparations were made in 5 formulas such as formula
A (blank), formula B (6% GSO), formula C (8% GSO), formula D (10% GSO),
formula E (12% GSO), combine with oxybenzone and octylmetoxycinnamate (2
and 5 % b/b) in each formulas. Evaluations conducted on cream homogenity,
emulsion type, pH, stability, skin irritation, and determining the SPF value of the
preparations by in vitro way using spectrophotometer UV. Then the value of SPF
will be tested in One Way Anova to see whether there is any significant
difference in those formulas.
Result: The evaluation results showed that all sample homogen with emulsion
type w/o, in range 6.53 – 6.56 pH, non-irritating, and a stable preparation during
12 weeks of storage at room temperature. The evaluation also showed that the
preparation’s SPF value increases along with the increased of additional grape
seed oil concentration, the average SPF value from each formula were : formula A
11.0714; formula B 11.9054; formula C 12.3189; formula D 12.7222; and
formula E 13.0343. These formulas can be used optimally as sunscreen with
maximal protection category. From the result of statistic tests, there was a
significant difference on the SPF value between 5 formulas’ preparation.

Conclusion: From the evaluation, be concluded that with additional of grape seed
oil
can
increase
the
effectiveness
sunscreen
preparation
from
octylmetoxycinnamate and oxybenzone in vanishing cream basis.
Key word: Sunscreen, Grape Seed Oil, Octylmetoxycinnamate, Oxybenzone, SPF
(Sun Protection Factor).

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