Pengaruh Pemberian Minyak Kelapa Murni dan Minyak Kedelai terhadap Profil Lipida Tikus Jantan (Rattus norvegicus).
Lampiran 1 . Bagan Alur Posedur Pembuatan Pakan Diet Tinggi Lemak
Pakan Diet Tinggi Lemak
81% Pakan Standar pellet 551 10% Lemak Kambing 1% Kuning Telur Dicampur rata sampai setengah padat
Dibentuk berupa silinder dengan ukuran diameter 0,5 cm panjang 1 cm.
Dikeringkan
Lampiran 2 . Bagan Alur Pengujian terhadap Profil Lipida Serum Darah Tikus
Tikus Diukur serum darah Diberi perlakuan selama 21 hari Diukur kadar profil lipida pada hari ke-1, 7, dan
14. Kadar profil lipida awal Kadar profil lipida
Lampiran 3 . Bagan Alur Pengambilan Serum Darah Tikus
Tikus Didislokasikan leher Dibedah diambil darah dari jantung Darah Tikus
Didiamkan selama 10 menit Disentrifugasi dengan kecepatan 3000 rpm selama 15 menit Diambil cairan bening
Serum siap diukur
Lampiran 4 . Bagan Alur Pengukuran Kadar Kolesterol Total
Reagen Kolesterol Diukur larutan blanko Diukur larutan standar Serum
Dipipet sebanyak 10 µl Masukkan dalam tabung reaksi yang berisi larutan reagen kolesterol sebanyak
1 ml Dihomogenkan Diinkubasi pada suhu kamar selama 20 menit Diukur dengan spektrofotometer mikrolab 300 pada panjang gelombang 546 nm
Dicatat hasilnya Kadar Kolesterol Total
Lampiran 5 . Bagan Alur Pengukuran Kadar Trigliserida
Reagen Trigliserida Diukur larutan blanko Diukur larutan standar Serum
Dipipet sebanyak 10 µl Masukkan dalam tabung reaksi yang berisi larutan reagen trigliserida sebanyak
1 ml Dihomogenkan Diinkubasi pada suhu kamar selama 20 menit Diukur dengan spektrofotometer mikrolab 300 pada panjang gelombang 546 nm
Dicatat hasilnya Kadar Trigliserida
Lampiran 6 . Bagan Alur Pengukuran Kadar HDL
200 µl larutan standart HDL Ditambah 500 µl pereaksi kolesterol yang telah diencerkan dengan akuades ( rasio 4 + 1 )
Diinkubasi pada suhu kamar selama 20
menit
Disentrifugasi dengan kecepatan 3000 rpm selama 15 menitLarutan supernatan Diukur larutan blanko Diukur larutan standar Serum Dipipet sebanyak 100 µl Dimasukkan dalam tabung reaksi yang berisi larutan reagen kolesterol HDL sebanyak 1 ml
Dihomogenkan Diinkubasi pada suhu kamar selama 20
menit
Diukur pada mikrolab 300 denganPanjang gelombang 546 nm Dicatat hasilnya Kadar HDL
Lampiran 7 . Data hasil Pengukuran Kadar lipida Tikus sebelum perlakuan
(mg/dl) Lipida Awal Non diet selama 21 hari diet selama 21 hari
71 77
84 Kolesterol total 79 80 80 77 82 83 174 214 219
Trigliserida
171 210 215 169 203 220 34 32
26 HDL 41 36 31 41 40
35
Lampiran 8 . Data hasil Pengukuran Kadar Kolesterol Total Tikus setelah perlakuan (mg/dl) Perlakuan Minggu 1 Minggu 2 minggu 3
63 65 84 ND 68 70 83
69 66 84 80 90 95 D 81 89 89
80 88 92 68 73 82 ND+VCO100 69 75 85
70 68 90 66 65 59 ND+MK100 62 65 62
64 67 61 76 86 83 D+VCO100 78 82 85
83 81 84 75 62 63 D+MK100 78 63 61
81 62 61 93 67 58 D+VCO75+MK25 89 65 61
96 67 64 58 45 43 D+VCO75+MK25 59 45 41
56 46 46 64 48 49 D+VCO50+MK50 65 58 53
64 55 51
Lampiran 9 . Data hasil Pengukuran Kadar Trigliserida Tikus setelah perlakuan (mg/dl) Perlakuan Minggu 1 Minggu 2 minggu 3
120 122 120 ND 128 124 135
127 120 125 190 200 205 D 194 208 203
192 204 207 99 85 72 ND+VCO100 98 96 80
140 71 82 68 63 63 ND+MK100 68 65 65
69 62 60 154 118 115 D+VCO100 150 120 108
167 116 96 140 130 120 D+MK100 138 128 117
139 132 123 93 67 58 D+VCO75+MK25 89 65 61
96 67 64 69 62 61 D+VCO75+MK25 68 62 62
67 62 50 84 68 53 D+VCO50+MK50 82 67 53
80 68 53
Lampiran 10 . Data hasil Pengukuran Kadar HDL Tikus setelah perlakuan (mg/dl) Perlakuan Minggu 1 Minggu 2 minggu 3
36 32 40 ND 38 32 38
37 40 36 39 43 46 D 36 45 43
39 42 44 38 45 65 ND+VCO100 39 46 65
40 44 64 42 41 38 ND+MK100 44 41 42
46 43 42 39 42 54 D+VCO100 41 50 51
41 54 58 43 33 30 D+MK100 41 32 31
44 32 32 37 49 45 D+VCO75+MK25 35 40 45
42 39 45 37 30 29 D+VCO75+MK25 36 31 25
35 32 32 43 33 35 D+VCO50+MK50 45 42 37
44 39 38
Lampiran 11 . Analisis Statistik Data Profil lipida Kontrol Awal
Kolmogorov-Smirnov Z ,524 ,727 ,499 Asymp. Sig. (2-tailed) ,946 ,666 ,965 a. Test distribution is Normal.
8 HDL Between Groups 99,556 2 49,778 2,835 ,136 Within Groups 105,333 6 17,556 Total 204,889
8 Trigliserida Between Groups 3677,556 2 1838,778 124,429 ,000 Within Groups 88,667 6 14,778 Total 3766,222
Kolesterol total Between Groups 67,556 2 33,778 3,619 ,093 Within Groups 56,000 6 9,333 Total 123,556
Sum of Squares df Mean Square F Sig.
2. Uji ANOVA dan uji lanjutan Duncan ANOVA
b. Calculated from data.
Most Extreme Differences Absolute ,175 ,242 ,166 Positive ,112 ,213 ,122 Negative -,175 -,242 -,166
1. Uji normalitas dengan menggunakan uji Kolmogorov-Smirnov One-Sample Kolmogorov-Smirnov Test
Mean 79,2222 199,4444 35,1111 Std. Deviation 3,92994 21,69741 5,06074
a,b
9 Normal Parameters
9
9
Kolesterol total Trigliserida HDL N
8
Lampiran 11 (lanjutan) Kolesterol Total
a
Duncan Subset for alpha = 0.05 perlakuan N
1
2 awal/normal 3 75,6667 nondiet selama 21 hari 3 79,6667 79,6667 diet selama 21 hari 3 82,3333
Sig. ,160 ,326 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Trigliserida
a
Duncan Subset for alpha = 0.05 perlakuan N
1
2
3 awal/normal 3 171,3333 nondiet selama 21 hari 3 209,0000 diet selama 21 hari 3 218,0000
Sig. 1,000 1,000 1,000
HDL
a
Duncan Subset for alpha =
0.05 perlakuan N 1 diet selama 21 hari
3 30,6667 nondiet selama 21 hari 3 36,0000 awal/normal 3 38,6667
Sig. ,065 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Lampiran 12 . Analisis Statistik Data Kolesterol Total
Most Extreme Differences Absolute ,145 ,141 ,221 Positive ,145 ,141 ,178 Negative -,071 -,124 -,221
26 KT minggu_3 Between Groups 7249,333 8 906,167 164,205 ,000 Within Groups 99,333 18 5,519 Total 7348,667
26 KT minggu_2 Between Groups 4308,074 8 538,509 84,045 ,000 Within Groups 115,333 18 6,407 Total 4423,407
KT minggu_1 Between Groups 2837,185 8 354,648 60,604 ,000 Within Groups 105,333 18 5,852 Total 2942,519
2. Uji ANOVA dan uji lanjutan Duncan ANOVA Sum of Squares df Mean Square F Sig.
b. Calculated from data.
Kolmogorov-Smirnov Z ,754 ,731 1,147 Asymp. Sig. (2-tailed) ,621 ,660 ,144 a. Test distribution is Normal.
Mean 72,4074 67,1481 69,2222 Std. Deviation 10,63832 13,04343 16,81193
1. Uji normalitas dengan menggunakan uji Kolmogorov-Smirnov One-Sample Kolmogorov-Smirnov Test
a,b
27 Normal Parameters
27
27
KT minggu_3 N
KT minggu_1 KT minggu_2
26
Lampiran 12 (lanjutan) Kolesterol Total minggu -1
a
Duncan Subset for alpha = 0.05 perlakuan N
1
2
3
4
5 D+VCO25+MK75 3 57,6667 ND+MK100 3 64,0000 D+VCO50+MK50 3 64,3333 ND 3 66,6667 66,6667 ND+VCO100 3 69,0000 D+MK100 3 78,0000 D+VCO100 3 79,0000 D 3 80,3333 D+VCO75+MK25
3 92,6667
Sig. 1,000 ,217 ,253 ,278 1,000 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Kolesterol Total minggu -2
a
Duncan Subset for alpha = 0.05 perlakuan N
1
2
3
4
5
6 D+VCO25+MK75 3 45,3333 D+VCO50+MK50 3 53,6667 D+MK100 3 62,3333 ND+MK100 3 65,6667 D+VCO75+MK25 3 66,3333 ND 3 67,0000 ND+VCO100 3 72,0000 D+VCO100
3 83,0000 D
3 89,0000
Sig. 1,000 1,000 ,051 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Lampiran 12 (lanjutan) Kolesterol Total minggu -3
Duncan
a
perlakuan N Subset for alpha = 0.05
1
2
3
4
5 D+VCO25+MK75 3 43,3333 D+VCO50+MK50 3 51,0000 ND+MK100 3 60,6667 D+VCO75+MK25 3 61,0000 D+MK100 3 61,6667 ND 3 83,6667 D+VCO100 3 84,0000 ND+VCO100 3 85,6667 D
3 92,0000
Sig. 1,000 1,000 ,628 ,337 1,000 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Lampiran 13 . Analisis Statistik Data Trigliserida 1
Uji normalitas dengan menggunakan uji Kolmogorov-Smirnov
One-Sample Kolmogorov-Smirnov Test
TG TG TG minggu_1 minggu_2 minggu_3 N
27
27
27
a,b
Normal Parameters Mean 115,1481 101,9259 96,7037 Std. Deviation 41,34350 45,37021 47,61126
Most Extreme Differences Absolute ,170 ,234 ,192 Positive ,170 ,234 ,192 Negative -,122 -,189 -,163
Kolmogorov-Smirnov Z ,886 1,215 ,996 Asymp. Sig. (2-tailed) ,413 ,105 ,274 a. Test distribution is Normal.
b. Calculated from data.
TG Between Groups 43051,407 8 5381,426 69,688 ,000 minggu_1 Within Groups 1390,000 18 77,222 Total 44441,407
26 TG Between Groups 53141,852 8 6642,731 316,321 ,000 minggu_2 Within Groups 378,000 18 21,000 Total 53519,852
26 TG Between Groups 58434,963 8 7304,370 261,562 ,000 minggu_3 Within Groups 502,667 18 27,926 Total 58937,630
26
Lampiran 13 (lanjutan) Trigliserida minggu -1
a
Duncan Subset for alpha = 0.05 perlakuan N
1
2
3
4
5
6 D+VCO25+MK75 3 68,0000 ND+MK100 3 68,3333 D+VCO50+MK50 3 82,0000 82,0000 D+VCO75+MK25 3 92,6667 ND+VCO100 3 112,3333 ND 3 125,0000 125,0000 D+MK100 3 139,0000 D+VCO100
3 157,0000 D
3 192,0000
Sig. ,080 ,154 ,094 ,067 1,000 1,000 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Trigliserida minggu -2
a
Duncan Subset for alpha = 0.05 perlakuan N
1
2
3
4
5 D+VCO25MK75 3 62,0000 ND+MK100 3 63,3333 D+VCO75+MK25 3 66,3333 D+VCO50+MK50 3 67,6667 ND+VCO100 3 84,0000 D+VCO100 3 118,0000 ND 3 122,0000 D+MK100 3 130,0000 D
3 204,0000
Sig. ,181 1,000 ,299 1,000 1,000 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Lampiran 13 (lanjutan) Trigliserida minggu -3
Duncan
a
perlakuan N Subset for alpha = 0.05
1
2
3
4
5 D+VCO50+MK50 3 53,0000 D+VCO25MK75 3 57,6667 D+VCO75+MK25 3 61,0000 ND+MK100 3 62,6667 ND+VCO100 3 78,0000 D+VCO100 3 106,3333 D+MK100 3 120,0000 ND 3 126,6667 D
3 205,0000 Sig. ,053 1,000 1,000 ,140 1,000 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Lampiran 14. Analisis Statistik Data HDL
Most Extreme Differences Absolute ,126 ,181 ,156 Positive ,126 ,181 ,156 Negative -,090 -,124 -,085
26 HDL minggu_3 Between Groups 3066,519 8 383,315 86,246 ,000 Within Groups 80,000 18 4,444 Total 3146,519
26 HDL minggu_2 Between Groups 877,630 8 109,704 8,511 ,000 Within Groups 232,000 18 12,889 Total 1109,630
HDL minggu_1 Between Groups 219,333 8 27,417 8,919 ,000 Within Groups 55,333 18 3,074 Total 274,667
ANOVA
Sum of Squares df Mean Square F Sig.b. Calculated from data.
Kolmogorov-Smirnov Z ,656 ,940 ,811 Asymp. Sig. (2-tailed) ,782 ,340 ,526 a. Test distribution is Normal.
Mean 39,8889 39,7037 42,5926 Std. Deviation 3,25025 6,53284 11,00091
One-Sample Kolmogorov-Smirnov Test
a,b
27 Normal Parameters
27
27
2 HDL minggu_3 N
HDL minggu_1 HDL minggu_
26
Lampiran 14 (lanjutan) HDL minggu -1
a
Duncan Subset for alpha = 0.05 perlakuan N
1
2
3
4 D+VCO25+MK75 3 36,0000 ND 3 37,0000 D 3 38,0000 38,0000 D+VCO75+MK25 3 38,0000 38,0000 ND+VCO100 3 39,0000 39,0000 D+VCO100 3 40,3333 40,3333 D+MK100 3 42,6667 42,6667 ND+MK100
3 44,0000
D+VCO50+MK50
3 44,0000
Sig. ,074 ,151 ,120 ,390 Means for groups in homogeneous subsets are displayed. Uses Harmonic Mean Sample Size = 3,000.
HDL minggu -2
a
Duncan Subset for alpha = 0.05 perlakuan N
1
2
3
4
5 D+VCO25MK75 3 31,0000 D+MK100 3 32,3333 32,3333 ND 3 34,6667 34,6667 D+VCO50+MK50 3 38,0000 38,0000 ND+MK100 3 41,6667 41,6667 D+VCO75+MK25 3 42,6667 42,6667 42,6667 D 3 43,3333 43,3333 43,3333 ND+VCO100 3 45,0000 45,0000 D+VCO100 3 48,6667 Sig. ,252 ,083 ,111 ,310 ,075 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Lampiran 14 (lanjutan) HDL minggu -3
Duncan
a
perlakuan N Subset for alpha = 0.05
1
2
3
4
5
6 D+VCO25+MK75 3 28,6667 D+MK100 3 31,0000 D+VCO50+MK50 3 36,6667 ND 3 38,0000 38,0000 ND+MK100 3 40,6667 D 3 44,3333 D+VCO75+MK25 3 45,0000 D+VCO100 3 54,3333 ND+VCO100
3 64,6667
Sig. ,192 ,449 ,139 ,703 1,000 1,000 Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Lampiran 15 Analisis statistik rasio kolesterol total dengan HDL
Test of Homogeneity of Variances
Levene Statistic df1 df2 Sig. minggu1 4.385
8 18 .004 minggu2 3.810
8 18 .009 minggu3 2.724
8 18 .037 ANOVA
Sum of Squares df Mean Square F Sig. minggu1 Between Groups 1.276 8 .160 15.954 .000 Within Groups .180
18 .010 Total 1.456 26 minggu2 Between Groups 4.012
8 .501 31.488 .000 Within Groups .287 18 .016 Total 4.299 26 minggu3 Between Groups 2.993
8 .374 59.426 .000 Within Groups .113 18 .006 Total 3.107
26 minggu1 a
Duncan Subset for alpha = .05
Perlakuan N
1
2
3
4 ND+MK100 3 1.4667 D+VCO50+MK50 3 1.4667 D+VCO75+MK25 3 1.5667 D+VCO25+MK75 3 1.6000 1.6000 D 3 1.7667 1.7667 D+MK100 3 1.8000 ND+VCO100 3 1.8000 D+VCO100 3 1.9333 ND
3 2.1667 Sig.
.150 .056 .076 1.000 Means for groups in homogeneous subsets are displayed.
a.
Uses Harmonic Mean Sample Size = 3.000.
Lampiran 15 (lanjutan)
minggu2
aDuncan Subset for alpha = .05
Perlakuan N
1
2
3 D+VCO100 3 1.2333 D+VCO50+MK50 3 1.2667 D+VCO25+MK75 3 1.3000 D+MK100 3 1.3333 D+VCO75+MK25 3 1.3667 ND+MK100 3 1.6000 D 3 1.7333 ND+VCO100 3 2.1667 ND 3 2.3333 Sig.
.259 .212 .123 Means for groups in homogeneous subsets are displayed.
a.
Uses Harmonic Mean Sample Size = 3.000.
minggu3
aDuncan Subset for alpha = .05
Perlakuan N
1
2
3 D 3 1.2000 D+VCO25+MK75 3 1.2000 ND+VCO100 3 1.2333 ND+MK100 3 1.2333 D+VCO75+MK25 3 1.2333 D+VCO50+MK50 3 1.2667 D+MK100 3 1.3000 1.3000 D+VCO100 3 1.4333 ND 3 2.3000 Sig.
.190 .054 1.000 Means for groups in homogeneous subsets are displayed.
a.
Uses Harmonic Mean Sample Size = 3.000.
Lampiran 16
Gambar Alat
Spektofotometer Microlab 300 ( Vital Scientific ) Sentrigufe ( Swing type model CD-50 SR Tomy Seiko )
Micropipet
Lampiran 17