Uji Sitotoksik Fraksi Etilasetat Daun Bangun-Bangun (Plectranthus amboinicus (Lour.) terhadap Larva Artemia salina Leach dengan Metode Brine Shrimp Lethality Test (BSLT)
UJI SITOTOKSIK FRAKSI ETILASETAT
DAUN BANGUN-BANGUN (Plectranthus amboinicus (Lour.) Spreng
TERHADAP LARVA Artemiasalina Leach DENGAN METODE
Brine Shrimp Lethality Test
ABSTRAK
Latar Belakang: Daun bangun-bangun (Plectranthus amboinicus (Lour.)
Spreng) merupakan salah satu tanaman yang berpotensi untuk dikembangkan
sebagai agen kemopreventif kanker.
Tujuan: Penelitian ini bertujuan untuk mengetahui efek sitotoksik fraksi
etilasetat daun bangun-bangun (FEDBB) terhadap larva Artemia salina Leach.
Metode: Penelitian eksperimentalini menggunakan 800 ekor larva udang
(Artemia salina Leach) yang dibagi menjadi 1 kelompok kontrol negatif dan 5
kelompok kontrol positif konsentrasi 10 µg/mL; 100 µg/mL dan 1000 µg/mL
fraksi etilasetat masing-masing terdiri dari 10 ekor larva dengan replikasi 5 kali.
Data hasil penelitian dianalisis secara ANAVA (analisis variansi) menggunakan
program SPSS (Statistical Product and Service Solution) versi 17.
Hasil:Hasil pengujian efek sitotoksik fraksi etilasetat secara berturut-turut
adalah fraksi etilasetat I nilai LC50 49,431 µg/mL, fraksi etilasetat II nilai LC50
42,955 µg/mL, fraksi etilasetat III nilai LC50 51,050 µg/mL, fraksi etilasetat IV
nilai LC50 72,945 µg/mL, fraksi etilasetat V nilai LC50 130,617 µg/mL.
Kesimpulan: Fraksi etilasetat II merupakan fraksi yang paling toksik terhadap
larva Artemia salina Leach karena mempunyai nilai LC50 yang paling rendah
diantara fraksi yang lain yaitu 42,955 µg/mL.
Kata kunci:
Plectranthus amboinicus (Lour.) Spreng, sitotoksik, Brine Shrimp
Lethality Test (BSLT), Larva Artemia salina
vii
Universitas Sumatera Utara
CYTOTOXIC EFFECT OF ETHYLACETATE FRACTION OF
BANGUN-BANGUN (Plectranthus amboinicus (Lour.) Spreng) LEAVES
ON Atremia salina Leach LARVA BY USING BRINE SHRIMP
LETHALITY METHOD
ABSTRACT
Background: Bangun-Bangun leaves (Plectranthus amboinicus (Lour.) Spreng is
one of the plants that have the potential to be developed as a cancer
chemopreventive agent.
The Purpose: The aim of the study were to find out the cytotoxic effect of
ethylacetate fraction bangun-bangun leaves (FEDBB) on Artemia salina Leach
larvae.
Methods: This experimental study using 800 Artemia salina Leach larvae were
divided into one control group negative 1 and 5 positive control group
concentration10 µg/mL; 100 µg/mL dan 1000 µg/mL of ethylacetate fraction each
consisting of 10 larvae with replication 5.The study cytotoxic effects of
ethylacetate fraction of bangun-bangun leaves were done on Artemia salina Leach
larvae using Brine Shrimp Lethality Test (BSLT) method. Data was analyzed by
ANOVA SPSS versi 17 analysis.
Result: Results of testing the cytotoxic effects of ethylacetate fraction in a row
ethylacetate fraction I LC50 49.431 µg/mL, ethylacetate fraction II LC50 42.955
µg/mL, ethylacetate fraction III LC50 51.050 µg/mL, ethylacetate fraction IV LC50
72.945 µg/mL, ethylacetate fraction V LC50 130.617 µg/mL.
Conclusion: The ethylacetate fraction II had the most toxic on Artemia salina
Leach larvae because of the lowest LC50 42.955 µg/mL.
Key words :
Plectranthus amboinicus (Lour.) Spreng, cytotocix, Brine Shrimp
Lethality Test (BSLT), Artemia salina larvae.
viii
Universitas Sumatera Utara
DAUN BANGUN-BANGUN (Plectranthus amboinicus (Lour.) Spreng
TERHADAP LARVA Artemiasalina Leach DENGAN METODE
Brine Shrimp Lethality Test
ABSTRAK
Latar Belakang: Daun bangun-bangun (Plectranthus amboinicus (Lour.)
Spreng) merupakan salah satu tanaman yang berpotensi untuk dikembangkan
sebagai agen kemopreventif kanker.
Tujuan: Penelitian ini bertujuan untuk mengetahui efek sitotoksik fraksi
etilasetat daun bangun-bangun (FEDBB) terhadap larva Artemia salina Leach.
Metode: Penelitian eksperimentalini menggunakan 800 ekor larva udang
(Artemia salina Leach) yang dibagi menjadi 1 kelompok kontrol negatif dan 5
kelompok kontrol positif konsentrasi 10 µg/mL; 100 µg/mL dan 1000 µg/mL
fraksi etilasetat masing-masing terdiri dari 10 ekor larva dengan replikasi 5 kali.
Data hasil penelitian dianalisis secara ANAVA (analisis variansi) menggunakan
program SPSS (Statistical Product and Service Solution) versi 17.
Hasil:Hasil pengujian efek sitotoksik fraksi etilasetat secara berturut-turut
adalah fraksi etilasetat I nilai LC50 49,431 µg/mL, fraksi etilasetat II nilai LC50
42,955 µg/mL, fraksi etilasetat III nilai LC50 51,050 µg/mL, fraksi etilasetat IV
nilai LC50 72,945 µg/mL, fraksi etilasetat V nilai LC50 130,617 µg/mL.
Kesimpulan: Fraksi etilasetat II merupakan fraksi yang paling toksik terhadap
larva Artemia salina Leach karena mempunyai nilai LC50 yang paling rendah
diantara fraksi yang lain yaitu 42,955 µg/mL.
Kata kunci:
Plectranthus amboinicus (Lour.) Spreng, sitotoksik, Brine Shrimp
Lethality Test (BSLT), Larva Artemia salina
vii
Universitas Sumatera Utara
CYTOTOXIC EFFECT OF ETHYLACETATE FRACTION OF
BANGUN-BANGUN (Plectranthus amboinicus (Lour.) Spreng) LEAVES
ON Atremia salina Leach LARVA BY USING BRINE SHRIMP
LETHALITY METHOD
ABSTRACT
Background: Bangun-Bangun leaves (Plectranthus amboinicus (Lour.) Spreng is
one of the plants that have the potential to be developed as a cancer
chemopreventive agent.
The Purpose: The aim of the study were to find out the cytotoxic effect of
ethylacetate fraction bangun-bangun leaves (FEDBB) on Artemia salina Leach
larvae.
Methods: This experimental study using 800 Artemia salina Leach larvae were
divided into one control group negative 1 and 5 positive control group
concentration10 µg/mL; 100 µg/mL dan 1000 µg/mL of ethylacetate fraction each
consisting of 10 larvae with replication 5.The study cytotoxic effects of
ethylacetate fraction of bangun-bangun leaves were done on Artemia salina Leach
larvae using Brine Shrimp Lethality Test (BSLT) method. Data was analyzed by
ANOVA SPSS versi 17 analysis.
Result: Results of testing the cytotoxic effects of ethylacetate fraction in a row
ethylacetate fraction I LC50 49.431 µg/mL, ethylacetate fraction II LC50 42.955
µg/mL, ethylacetate fraction III LC50 51.050 µg/mL, ethylacetate fraction IV LC50
72.945 µg/mL, ethylacetate fraction V LC50 130.617 µg/mL.
Conclusion: The ethylacetate fraction II had the most toxic on Artemia salina
Leach larvae because of the lowest LC50 42.955 µg/mL.
Key words :
Plectranthus amboinicus (Lour.) Spreng, cytotocix, Brine Shrimp
Lethality Test (BSLT), Artemia salina larvae.
viii
Universitas Sumatera Utara