Sex reversal of Nile tilapia using honey and analysis of aromatase gene expression
i
SEX REVERSAL IKAN NILA MENGGUNAKAN MADU DAN
ANALISIS EKSPRESI GEN AROMATASE
ENY HERIYATI
MAYOR ILMU AKUAKULTUR
SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
2012
ii
iii
PERNYATAAN MENGENAITESISDAN SUMBER
INFORMASI
Dengan ini saya menyatakan bahwa tesis “Sex Reversal Ikan Nila Menggunakan
Madu dan Analisis Ekspresi Gen Aromatase” adalah karya saya dengan arahan
dari komisi pembimbing dan belum diajukkan dalam bentuk apa pun kepada
perguruan tinggi mana pun. Sumber informasi yang berasal atau dikutip dari karya
yang diterbitkan maupun tidak diterbitkan dari penulis lain telah disebutkan dalam
teks dan dicantumkan dalam Daftar Pustaka di bagian akhir tesis ini.
Bogor, September 2012
Eny Heriyati
C151100181
iv
v
ABSTRACT
ENY HERIYATI.Sex reversal of Nile tilapia using honey and analysis of
aromatase gene expression.Supervised by ALIMUDDIN and HARTON ARFAH
The use of synthetic steroid hormones to produce monosex populations of
tilapia for intensive productive systems may lead to environmental and public
health concerns. Such a situation is derived from the fact that female tilapine
species have a high fecundity, generally reproducing at a small size and exhibiting
stunted somatic growth at higher densities, while male tilapias exhibit faster
growth rates and are often the preferred gender for monosex aquaculture.The
study was conducted to determine the effect of immersion Nile tilapia larvae in
water containing different honey source on male fish percentage and aromatase
gene expression. In the first experiment, in a total of 30 tilapia larvae at 12 days
posthatch were immersed in the water containing honey derived from the forest,
cultured and mangrove bees, with a dose of 10 mL/L water for 10 hours. Fish
were then maintained in the same condition for 2 months. The results showed that
percentage of male fish immersed by different honey was similar (p>0.05), and all
treatment was significantly different with the control (p
SEX REVERSAL IKAN NILA MENGGUNAKAN MADU DAN
ANALISIS EKSPRESI GEN AROMATASE
ENY HERIYATI
MAYOR ILMU AKUAKULTUR
SEKOLAH PASCASARJANA
INSTITUT PERTANIAN BOGOR
2012
ii
iii
PERNYATAAN MENGENAITESISDAN SUMBER
INFORMASI
Dengan ini saya menyatakan bahwa tesis “Sex Reversal Ikan Nila Menggunakan
Madu dan Analisis Ekspresi Gen Aromatase” adalah karya saya dengan arahan
dari komisi pembimbing dan belum diajukkan dalam bentuk apa pun kepada
perguruan tinggi mana pun. Sumber informasi yang berasal atau dikutip dari karya
yang diterbitkan maupun tidak diterbitkan dari penulis lain telah disebutkan dalam
teks dan dicantumkan dalam Daftar Pustaka di bagian akhir tesis ini.
Bogor, September 2012
Eny Heriyati
C151100181
iv
v
ABSTRACT
ENY HERIYATI.Sex reversal of Nile tilapia using honey and analysis of
aromatase gene expression.Supervised by ALIMUDDIN and HARTON ARFAH
The use of synthetic steroid hormones to produce monosex populations of
tilapia for intensive productive systems may lead to environmental and public
health concerns. Such a situation is derived from the fact that female tilapine
species have a high fecundity, generally reproducing at a small size and exhibiting
stunted somatic growth at higher densities, while male tilapias exhibit faster
growth rates and are often the preferred gender for monosex aquaculture.The
study was conducted to determine the effect of immersion Nile tilapia larvae in
water containing different honey source on male fish percentage and aromatase
gene expression. In the first experiment, in a total of 30 tilapia larvae at 12 days
posthatch were immersed in the water containing honey derived from the forest,
cultured and mangrove bees, with a dose of 10 mL/L water for 10 hours. Fish
were then maintained in the same condition for 2 months. The results showed that
percentage of male fish immersed by different honey was similar (p>0.05), and all
treatment was significantly different with the control (p