Lampiran 2 Pola difraksi sinar X a. Kondisi proses, kristanlitas dan grafik pola difraksi MOCAF native
b. Kondisi proses, kristanlitas dan grafik pola difraksi Mocaf termodifikasi 1 siklus
Pola Difraksi Sinar X Kristalin Mocaf native
c. Kondisi proses, kristanlitas dan grafik pola difraksi Mocaf termodifikasi 2 siklus
Pola Difraksi Sinar X Kristalin MOCAF termodifikasi 1 siklus
d. Kondisi proses, kristanlitas dan grafik pola difraksi Mocaf termodifikasi 3 siklus
Pola Difraksi Sinar X Kristalin MOCAF termodifikasi 2 siklus
Pola Difraksi Sinar X Kristalin Mocaf termodifikasi 3 siklus
Lampiran 3 Daya cerna Mocaf native dan Mocaf termodifikasi a. Kurva Standar Maltosa
Konsentrasi ABS
0.1 0.098
0.2 0.241
0.3 0.393
0.4 0.548
0.5 0.713
b. Perhitungan kadar maltosa Kadar maltosa =
dimana : Abs = absorban a = 0.0298
b = 1.4477
Abs Maltosa
mgL Mocaf native A
0.369 0.275471437
Blanko Mocaf native A 0.005
0.024038129 Pati murni A
0.431 0.31829799
Blanko pati murni A 0.008
0.026110382 Mocaf native B
0.371 0.276852939
Blanko Mocaf native B 0.005
0.024038129 Pati murni B
0.432 0.318988741
Blanko pati murni B 0.009
0.026801133
y = 1.4477x - 0.0298 R² = 0.9945
-0.2 0.2
0.4 0.6
0.8
0.1 0.2
0.3 0.4
0.5 0.6
Abso rb
a n
si
Konsentrasi Maltosa mgL
Standar Maltosa
Kadar maltosa Mocaf termodifikasi Ulangan 1
Sampel Abs
Maltosa mgL MS1A
0.334 0.251295158
MS1B 0.334
0.251295158 MS2A
0.313 0.23678939
MS2B 0.314
0.237480141 MS3A
0.305 0.231263383
MS3B 0.306
0.231954134 Blanko
Abs Maltosa mgL
MS1A 0.032
0.042688402 MS1B
0.032 0.042688402
MS2A 0.028
0.039925399 MS2B
0.039 0.047523658
MS3A 0.041
0.04890516 MS3B
0.038 0.046832907
Kadar maltosa Mocaf termodifikasi Ulangan 2
Sampel Abs
Maltosa mgL MS1A
0.333 0.250604407
MS1B 0.337
0.25336741 MS2A
0.316 0.238861643
MS2B 0.315
0.238170892 MS3A
0.307 0.232644885
MS3B 0.306
0.231954134 Blanko
Abs Maltosa mgL
MS1A 0.036
0.045451406 MS1B
0.044 0.050977412
MS2A 0.035
0.044760655 MS2B
0.026 0.038543897
MS3A 0.041
0.04890516 MS3B
0.037 0.046142157
MS1 : Mocaf termodifikasi 1 Siklus MS2 : Mocaf termodifikasi 2 Siklus
MS3 : Mocaf termodifikasi 3 Siklus A B : Duplo
c. Persentase daya cerna Mocaf native dan Mocaf termodifikasi Daya cerna =
x100
Daya Cerna Mocaf native
Maltosa Daya Cerna
DC Sampel
Blanko Pati Murni
Blanko A-B
C-D A
B C
D 0.275471
0.024038129 0.318298
0.02611 0.251433
0.292188 0.8605201
86.1
0.276853 0.024038129
0.3189887 0.026801
0.252815 0.292188
0.8652482 86.5
Rata-rata 86.29
Daya Cerna Mocaf termodifikasi ulangan 1
KODE Maltosa
Daya Cerna
DC RATA2
Sampel Blanko
Pati Murni
Blanko A-B
C-D A
B C
D MS1A
0.25129 0.04268840
0.31829 0.0261
0.20860 0.29218
0.714 71.4
71.4
MS1B 0.25129
0.04268840 0.20860
0.714 71.4
MS2A 0.23678
0.03992539 0.19686
0.674 67.4
66.2
MS2B 0.23748
0.04752365 0.18995
0.650 65.0
MS3A 0.23126
0.04890516 0.18235
0.624 62.4
62.8
MS3B 0.23195
0.04683290 0.18512
0.634 63.4
Daya Cerna Mocaf termodifikasi ulangan 2
KODE Maltosa
Daya Cerna
DC RATA2
Sampel Blanko
Pati Murni
Blanko A-B
C-D A
B C
D MS1A
0.25060 0.04545140
0.31829 0.0261
0.20515 0.29218
0.702 70.2
69.7 MS1B
0.25336 0.05097741
0.20239 0.693
69.3 MS2A
0.23886 0.04476065
0.19410 0.664
66.4 67.4
MS2B 0.23817
0.03854389 0.19962
0.683 68.3
MS3A 0.23264
0.04890516 0.18374
0.629 62.9
63.2 MS3B
0.23195 0.04614215
0.18581 0.636
63.6
Daya Cerna RATA
SD Mocaf native ul1
86.1 86.28842 0.334329
Mocaf native ul2 86.5
Mocaf 1 siklus ul1 71.3948
70.56738 1.170153 Mocaf 1 siklus ul2
69.73995 Mocaf 2 siklus ul1
66.19385 66.78487 0.835824
Mocaf 2 siklus ul2 67.37589
Mocaf 3 siklus ul1 62.88416
63.06147 0.250747 Mocaf 3 siklus ul2
63.23877
d. Analisis of Variance dan Uji Lanjut Tukey Daya Cerna
a. Anova
DAYA CERNA Sum of Squares
df Mean Square
F Sig.
Between Groups 626.776
3 208.925
380.089 0.000
Within Groups 2.199
4 .550
Total 628.975
7
b. Uji Lanjut
Tukey HSD
a
SAMPEL N
Subset for alpha = 0.05 1
2 3
4 MOCAF 3 siklus
2 63.0550
MOCAF 2 siklus 2
66.7800 MOCAF 1 siklus
2 70.5650
MOCAF native 2
86.3000 Sig.
1.000 1.000
1.000 1.000
Lampiran 4 Derajat Putih Mocaf native dan Mocaf termodifikasi Warna =
x100 a. Hasil pengukuran warna dan warna pada BaSO
4
, Mocaf native dan Mocaf termodifikasi.
Sampel Warna
Warna BaSO
4
A 81.6
100 BaSO
4
B 81.6
100 Rata2
81.6 100
SD Mocaf native A
93.9 115.07
Mocaf native B 93.8
114.95 Rata2
93.85 115.01
SD 0.07
0.09 Mocaf 1 siklus A
77.5 94.98
Mocaf 1 siklus B 76
93.14 Rata2
76.75 94.06
SD 1.06
1.30 Mocaf 2 siklus A
81 99.26
Mocaf 2 siklus B 77.6
95.10 Rata2
79.3 97.18
SD 2.40
2.95 Mocaf 3 siklus A
80.8 99.02
Mocaf 3 siklus B 79.9
97.92 Rata2
80.35 98.47
SD 0.63
0.78
b. Analysis of variance dan uji lanjut Tukey warna pada BaSO
4
, Mocaf native dan Mocaf termodifikasi.
1. Anova
WARNA Sum of Squares
df Mean Square
F Sig.
Between Groups 532.843
4 133.211
60.783 0.000
Within Groups 10.958
5 2.192
Total 543.800
9
2. Uji Lanjut Warna
Tukey HSD
a
SAMPEL N
Subset for alpha = 0.05 1
2 3
Mocaf 1 siklus 2
94.0600 Mocaf 2 siklus
2 97.1800
97.1800 Mocaf 3 siklus
2 98.4700
98.4700 BaSO4
2 100.0000
Mocaf native 2
115.0100 Sig.
0.138 0.417
1.000
Lampiran 5 Amylose Leaching Mocaf native dan Mocaf termodifikasi a. Kurva Standar Amilosa
konsentrasi Abs
0.0025 0.019
0.005 0.035
0.0075 0.052
0.01 0.068
0.0125 0.083
0.015 0.102
b. Perhitungan Konsentrasi Amilosa dalam sampel dan Amylosa leaching. Konsentrasi sampel Ks =
dimana : Abs = Absorbansi a = 0.0013
b = 6.6714
Amylose leaching AML = x100
dimana : Fp = Faktor pengenceran
Ks = Konsentrasi sampel
W = Berat sampel mg
y = 6.6714x + 0.0012 R² = 0.9992
0.02 0.04
0.06 0.08
0.1 0.12
0.002 0.004
0.006 0.008
0.01 0.012
0.014 0.016
A b
sor b
an si
Konsentrasi Amilosa
Sampel W
Abs Ks
Fp AML
Mocaf native 1 100.2
0.026 0.00363
11.765 4.25673
Mocaf native 1 100.2
0.026 0.00363
11.765 4.25673
Sampel W
Abs Ks
Fp AML
MS1U1A 135.4
0.019 0.00265
11.765 2.30525
MS1U1B 127.3
0.018 0.00250
11.765 2.31341
MS1U2A 104.5
0.016 0.00220
11.765 2.48065
MS1U2B 104
0.017 0.00235
11.765 2.66214
MS2U1A 129.8
0.016 0.00220
11.765 1.99713
MS2U1B 133.3
0.017 0.00235
11.765 2.07699
MS2U2A 102.7
0.014 0.00190
11.765 2.18071
MS2U2B 100.3
0.014 0.00190
11.765 2.23289
MS3U1A 119.8
0.014 0.00190
11.765 1.86944
MS3U1B 121.2
0.015 0.00205
11.765 1.99334
MS3U2A 104.6
0.015 0.00205
11.765 2.30969
MS3U2B 103.5
0.014 0.00190
11.765 2.16385
c. Analysis of variance dan uji lanjut Tukey Amylose leaching pada Mocaf native dan Mocaf termodifikasi.
1. Anova
Amylose Leaching Sum of Squares
df Mean Square
F Sig.
Between Groups 6.396
3 2.132
88.919 .000
Within Groups .096
4 .024
Total 6.491
7 MS1U1: Mocaf termodifikasi 1 Siklus Ulangan 1
MS1U2: Mocaf termodifikasi 1 Siklus Ulangan 2 MS2U1: Mocaf termodifikasi 2 Siklus Ulangan 1
MS2U2 : Mocaf termodifikasi 2 Siklus Ulangan 2 MS3U1 : Mocaf termodifikasi 3 Siklus ulangan 1
MS3U2 : Mocaf termodifikasi 3 Siklus ulangan 2 A B : Duplo
2. Descriptives Rata-rata dan standar deviasi
N Mean
Std. Deviation Mocaf native
2 4.2500
.00000 Mocaf 1 siklus
2 2.4350
.19092 Mocaf 2 siklus
2 2.1150
.12021 Mocaf 3 siklus
2 2.0800
.21213 Total
8 2.7200
.96299
3. Uji lanjut
Tukey HSD
a
SAMPEL N
Subset for alpha = 0.05 1
2 Mocaf 3 siklus
2 2.0800
Mocaf 2 siklus 2
2.1150 Mocaf 1 siklus
2 2.4350
Mocaf native 2
4.2500 Sig.
.243 1.000
Lampiran 6 Sineresis Mocaf native dan Mocaf termodifikasi Sineresis =
x100 dimana : A
= Air yang keluar dari matriks gel gr W
= Berat sampel gr
KODE SAMPEL BERAT
SINERESIS Wgr
A gr Mocaf native A
10.012 2.8153
28.11925689 Mocaf native B
10.0119 2.7987
27.95373506 MS1U1A
10.0288 4.36
43.4747926 MS1U1B
10.0284 4.3
42.87822584 MS1U2A
10.0555 4.46
44.35383621 MS1U2B
10.0368 4.4
43.83867368 MS2U1A
10.0551 5.32
52.90847431 MS2U1B
10.0682 5.2472
52.11656503 MS2U2A
10.0262 5.19
51.76437733 MS2U2B
10.0205 5.302
52.91153136 MS3U1A
10.0287 5.8
57.83401637 MS3U1B
10.0586 5.91
58.75569165 MS3U2A
10.0455 5.89
58.63321885 MS3U2B
10.0414 5.87
58.45798395
Analysis of variance dan uji lanjut TUKEY Sineresis pada Mocaf native dan Mocaf termodifikasi.
A. Descriptives Rata-rata dan standar deviasi
N Mean
Std. Deviation Mocaf native
2 28.0400
.00000 Mocaf 1 siklus
2 43.6400
.65054 Mocaf 2 siklus
2 52.4250
.12021 Mocaf 3 siklus
2 58.4200
.18385 Total
8 45.6313
12.22830
B. Anova
