3.2. Materials and equipment
Table 2 List of field materials and equipments
Equipment Utility
Material Utility
IR-Non contact thermometer
Conventional thermometer
Temperature measurement Infra red sensor
Temperature measurement alcohol
Ethanol 70
Preservation for seaweed, seagrass and
dead hatchlings
Roll meter Length and distance
Inconel tag Flipper tag Hand held GPS
Positioning Measuring tape
flexible and stiff Seaturtle morphometric
measurement Caliper
Hatchling body measurement Digital Scale
Hatchling body mass measurement
Ziplock bag Sand and seaweed
samples storage Styrofoam box
Seaweed and dead hatchlings samples storage
Shovel Head light
Sand sampler Nightly measurement
∗
see Appendix 1a
3.3. Data collection
Table 3 List of data collection
Data Note
Primary Data
Nesting beach characteristic: - Sand grain size composition
Field sampling; samples were analyzed at Soil Laboratoty in Dept. Tanah, Faperta-IPB
- Beach shade area Field measurement
- Beach vegetation Field sampling, samples were identified at LIPI
Botani Nest natural artificial characteristic:
- Nest position relative to beach segments and vegetation
Field observation and measurement - Nest and sand temperature
Field measurement - Nest depth
Field measurement - Sand grain size composition
Field sampling, samples were analyzed at Environment Laboratory, Dept. BDP, FPIK-IPB
- Nest humidity Field sampling, samples were analyzed at
Marine Biology Laboratory at Dept. ITK, FPIk- IPB
Seaturtle morphometric adult and hatchling
Field measurement Seaturtle track
Field measurement
Table 3 continue
Secondary Data
Population data UPTD Konservasi Penyu Pangumbahan
Beach profile Yonathan 2010, unpublished data
Sea bottom substrate http:edcsns17.cr.usgs.govEarthExplorer
Monthly SST April 2010 2011 and Oct 2010 2011
http:www.oceancolor.gsfc.nasa.gov
3.4.
Sampling methods 3.4.1.
Seaturtle body measurement and tagging
Adult female nesting seaturtle body measurement and flipper tag attachment were done after the oviposition was done. Morphometric measurements were done
in two ways, Curve Carapace Length and Width CCLW and Straight Carapace Length and Width SCLW Bolten, 1999 see Figure 3. Measurements over the
carapace curve CCL in adults are 3 to 4 cm larger than straight carapace length SCL Marquez-M, 1990. CCLW was measured by using flexible measuring
tape while Straight Carapace Length and Width SCLW was measured by using stiff measuring tape and stick.
Straight carapace length and width SCLW Bolten, 1999 of 21 nesting green turtles and curved carapace length and width CCLW Bolten, 1999 of 25
green turtles were measured during the field work. Additional data of curved carapace length and width CCLW and population data of nesting green turtle in
2010 n = 104 and 2011 n = 36 were obtained from the UPTD Konservasi Penyu Pangumbahan. Hatchlings carapace length and width n = 58 were
measured by using SCLW as suggested by Bolten 1999. Hatchlings body weight n = 55 was measured by using digital scale accuracy ± 0.01 gram.
In addition, we also investigated hatchlings straight carapace length - width and body weight. Straight carapace size was measured using a caliper with
accuracy of ± 0.1 cm. The main interest for this measurement was to know whether it was safe to sample hatchlings body fluids for sex determination. Sex of
seaturtle hatchlings can not be defined by looking at external anatomy. There are several methods to do so, both direct and indirect method. Conventional method
was to look at the gonad by dissecting. Testosterone reproduction hormone level assay by using blood tissue allows us to define hatchlings sex without harming
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