them. Initially, it has to be confirmed that the sampled blood volume did not exceed 1 of the body weight Phillippa, 2011, personal communication.
3.4.2. Habitat characteristic
3.4.2.1. Nesting habitat
Main focus of this section was to observe the characteristic of green turtle C. mydas nesting beach of Pangumbahan, Sukabumi and nests both natural and
artificial characteristics. Characteristics which were studied in this research were beach profile, beach and hatcheries shade area, beach and nest sand grain
composition, nest temperature, nest humidity, and nest depth.
3.4.2.1.1. Nesting beach profile
Beach profile information was obtained from a research partner, Yonathan 2010, unpublished data who mapped the beach topography by using theodolith.
General information of beach profile such as slope and width also obtained with direct observation during sampling qualitatively.
3.4.2.1.2. Beach and hatchery shade area condition
There are reports noted that many green turtle nest located under canopy of beach vegetation, especially Sea pandanus Nuitja, 1992. The vegetation also
plays role in regulating humidity of the substrate surrounding. Some female turtles prefer to lay eggs under the canopy of vegetation.
Line intersect sampling method van Laar and Akça, 2007, see Figure 5, was carried out to measure the shade area of vegetation and sample domination
type and taxa of outer beach vegetation Diez and Ottenwalder, 1999. A few meters extension toward the vegetation aggregation was applied also as we
learned from our preliminary survey that green turtle of Pangumbahan also found nesting there. Type of vegetation was be classified as tree and shrub.
Figure 5 Line intercept sampling method van Laar and Akça, 2007. Lines stretched along the outer vegetation as long as 50 meters black stripes
with intervals of 50 meters along the outer vegetation dash line. Extensions were made a few meters inside the vegetation where the nest
frequently found. Note: Hatchery A: open cage hatchery; hatchery B: Asbestos roofed hatchery; UC: under canopy area; NC: No canopy area
and HWM: near High Water Mark area
Shade areas of hatcheries were not quantitatively measured in detail like in nesting beach. It was only qualitative observation. There were four hatcheries
facilities with different canopy. Two hatcheries were not built with roofs which were patchily shaded by vegetation canopy. The rest were built with roof, one was
built with asbestos roof and the other was with fiber roof. The roof provides shade evenly at all time of day.
3.4.2.1.3. Substrate of beach and nests
Surface sand was sampled at each beach segment, natural nest and artificial nest in order to get information for its grain size composition. Sand grain size
composition of Pangumbahan nesting beach was sampled at each beach segmentPos. Especially for Pos 2, samples were taken twice, Pos 2 left hand side
of the hatchery 2A and at right hand side of the hatchery 2B. Sand sample were taken at under canopy area UC, without canopy area NC and 1 sample at
near high water mark HWM. 13
