Potensi gen bmpr-1b dan bmp-15 sebagai penciri untuk seleksi sifat prolifik pada domba ekor gemuk (DEG) Lombok
POTENSI GEN BMPR-1B DAN BMP-15 SEBAGAI PENCIRI
UNTUK SELEKSI SIFAT PROLIFIK PADA DOMBA EKOR
GEMUK (DEG) LOMBOK
TAPAUL ROZI
SEKOLAH PASCA SARJANA
INSTITUT PERTANIAN BOGOR
BOGOR
2011
PERNYATAAN MENGENAI TESIS DAN
SUMBER DAYA INFORMASI
Dengan ini saya menyatakan bahwa tesis yang berjudul Potensi Gen
BMPR-1B dan BMP15 sebagai Penciri untuk Seleksi Sifat Prolifik pada Domba
Ekor Gemuk (DEG) Lombok adalah karya saya dengan arahan dari komisi
pembimbing dan belum diajukan dalam bentuk apapun kepada perguruan tinggi
manapun. Sumber informasi yang berasal atau dikutip dari karya yang diterbitkan
maupun tidak diterbitkan dari penulis lain telah disebutkan dalam teks dan
dicantumkan dalam Daftar Pustaka di bagian akhir tesis ini.
Bogor, Februari 2011
Tapaul Rozi
NRP. D151080201
ABSTRACT
TAPAUL ROZI. Potential of BMPR-1B and BMP-15 genes as Marker Selection
for Prolification in The Lomboknese Fat Tail Sheep (LFTS). Under supervised of
MULADNO and JAKARIA
The Lomboknese fat tailed sheep (LFTS) is a local sheep that have been
long time raised and well adapted to the extreme environments of Lombok island.
The aim of this study was to identify the BMPR-1B and BMP-15 genes and their
influences on the prolification of the LFTS. Polymorphism of BMPR-IB and
BMP-15 genes were identified with restriction enzymes AvaII and HinfI. Data
were analyzed based on allele frequencies and t test was applied to compare the
mean values of litter size in different genotypes. The results of study showed that
polymorphism of BMPR-1B gene resulted in three genotypes, namely : ++ (140
bp/140 bp), B+ (140 bp/109 bp/31 bp) and BB (109 bp/31 bp) with allele + and B
frequencies were 0.839 and 0.161, respectively. While BMP-15 gene resulted in
two genotypes, namely ++ (110 bp/110 bp) and G+ (141 bp / 110 bp) with allele
G and + frequencies were 0.946 and 0.054, respectively. Chi-square test for
confirming the Hardy-Weinberg equilibrium showed that both BMPR-1B and
BMP-15 genes were in the equilibrium conditions. A highly significant difference
(P
UNTUK SELEKSI SIFAT PROLIFIK PADA DOMBA EKOR
GEMUK (DEG) LOMBOK
TAPAUL ROZI
SEKOLAH PASCA SARJANA
INSTITUT PERTANIAN BOGOR
BOGOR
2011
PERNYATAAN MENGENAI TESIS DAN
SUMBER DAYA INFORMASI
Dengan ini saya menyatakan bahwa tesis yang berjudul Potensi Gen
BMPR-1B dan BMP15 sebagai Penciri untuk Seleksi Sifat Prolifik pada Domba
Ekor Gemuk (DEG) Lombok adalah karya saya dengan arahan dari komisi
pembimbing dan belum diajukan dalam bentuk apapun kepada perguruan tinggi
manapun. Sumber informasi yang berasal atau dikutip dari karya yang diterbitkan
maupun tidak diterbitkan dari penulis lain telah disebutkan dalam teks dan
dicantumkan dalam Daftar Pustaka di bagian akhir tesis ini.
Bogor, Februari 2011
Tapaul Rozi
NRP. D151080201
ABSTRACT
TAPAUL ROZI. Potential of BMPR-1B and BMP-15 genes as Marker Selection
for Prolification in The Lomboknese Fat Tail Sheep (LFTS). Under supervised of
MULADNO and JAKARIA
The Lomboknese fat tailed sheep (LFTS) is a local sheep that have been
long time raised and well adapted to the extreme environments of Lombok island.
The aim of this study was to identify the BMPR-1B and BMP-15 genes and their
influences on the prolification of the LFTS. Polymorphism of BMPR-IB and
BMP-15 genes were identified with restriction enzymes AvaII and HinfI. Data
were analyzed based on allele frequencies and t test was applied to compare the
mean values of litter size in different genotypes. The results of study showed that
polymorphism of BMPR-1B gene resulted in three genotypes, namely : ++ (140
bp/140 bp), B+ (140 bp/109 bp/31 bp) and BB (109 bp/31 bp) with allele + and B
frequencies were 0.839 and 0.161, respectively. While BMP-15 gene resulted in
two genotypes, namely ++ (110 bp/110 bp) and G+ (141 bp / 110 bp) with allele
G and + frequencies were 0.946 and 0.054, respectively. Chi-square test for
confirming the Hardy-Weinberg equilibrium showed that both BMPR-1B and
BMP-15 genes were in the equilibrium conditions. A highly significant difference
(P