untuk
konsentrasi krioprotektan EG adalah: Y
=
+
46,
untuk konsentrasi DMSO mempunyai
Y
=
+
tidak
Y
adalah
spermatozoa pascapencairan
dan
X adalah
untuk konsentrasi krioprotektan EG atau
DMSO.
Hasil menunj
uk
kan
bahwa,
jenis krioprotektan
baik maupun
DMSO dapat dipakai untuk
semen EG yang dapat dipakai
untuk
4 , 6 atau
8,
sedangkan DMSO
sebaiknya
4
atau
6.
Ethylene
glycol
EG pada taraf konsentrasi
4,6
dan 8 dapat sebagai
dan tidak
fat
spermatozoa.
pula
DMSO
pada
taraf
4
dan
6
dapat dipakai
sebagai
krioprotektan pada taraf
8, DMSO
toksiknya terhadap viabilitas
spermatozoa pada
suhu
SEM, sperma yang
baik DMSO maupun
EG, secara menunjukkan
di
bagian ekor
sebagian
bagian
ekor membran plasma ekor
dan sebagian membran akrosom
atau bagian
akrosom lepas bagian
anterior kepala sperma.
dan
dan d m
Kontrak No. 0
1 1
SUMMARY
EFFECTMTY
OF
CRYOPROTECTANTS AND EVALUATION OF FROZEN
SPERM QUALITY
AS
A
O N
GENE POOL
PRESERVATION OF
PROPAGATED
GREEN JUNGLE
CALLUS Rita
ti,
Nia 2000,
+
34
pages
application of frozen
technique using the method
has
been
widely used for
cow
and to a
certain
extend in
sheep
and goats. Studies on
frozen sperm in
are
very
limited, mostly in
Local
fowl and quails, however,
no
study of this sort has
ever been
conducted
in the Green jungle
fowl.
The common cryoprotectant
for frozen ruminant
i s
glycerol. Procedures using glycerol for
poultry sperm
resulted
in good
sperm
quality thawing,
however,
with
low
fertilizing capacity. For these
reasons
it
i s
necessary to investigate the
suitable
cryoprotectant
for freezing poultry semen.
From
previous experience with frozen Local fowl sperm
6
ethylene glycol or
dimethylsulphoxide DMSO could be utilized as
for freezing
Green Jungle fowl sperm,
however,
the
optimal concentration, effectivity, efficiency and economically, remains to
be investigated. The damage,
resulted by
the freezing
as
indicated
by sperm abnormalities has only been
observed by microscopy. More
study on the sperm freeze
thaw
damage should be
explored by using the
electron microscopy. experiment
has
been conducted
to
obtain
a method
for Green Jungle fowl frozen
sperm,
by the most
cryoprotectant
with
optimal concentrat ion. The
frozen-thawed semen posses high quality suitable for
with high
motility, morphology, viability
and
fertilizing capacity. Observation and more detailed
study
on the
of frozen
sperm
conducted
to determine the
freeze-thaw damage. The factorial
2x3
completely randomized design was used with 2
factors
EG and
DMSO and 3 levels
of
concentration
and
8
and
3 times replications.
Data
were analyzed using the
analysis of
variance
followed by the Duncan multiple range test.
Semen was collected and pooled from Green Jungle fowls induced with
40
PMSG .m. Fresh
semen quality posses
the
following characteristics: ejaculate volume
v
of
0.01
4
0.001 colour,
viscous, pH
7.27
0.01 8
and microscopically showed mass
movement
of
1.0 ,
concentration
3.036
billions
per and
10.47
0.479 abnormality.
Frozen-thawed semen
motility
of the Green Jungle fowl
is
lowest
8
DMSO
1
and highest
in
EG Lowest
abnormality
was
found in frozen
in 6
EG
8.50
and highest
8
DMSO during
2 hours at was 2.22
from
spermatozoa
frozen in DMSO
and in
4
EG. Frozen-thawed semen
decrease motility from 13.89 to 3
with ranging
7.1
8 to 65.
The decrease in motility
and
increase in abnormality of
spermatozoa
was
resulted in semen
frozen 8
DMSO and differ significantly
either between all concentrations 4, 6
and
8
or
EG
or
DMSO
method in
the
doses determination, the equation for EG
i s
46,
the equation
for
DMSO is +
-
I
The
axis
denotes
the
post-thaw
sperm
and the X
axis
denotes the EG
DMSO
results show that either EG
or DMSO could be used as for
Green Jungle fowl.
Ethylene glycol EG
at
concentrations of
4, 6
and 8
could be
used safely as
DMSO could only be used
y at
concentration of
4
and
6,
while 8
DMSO
shows
toxic
effects
to sperm
viability
Faculty Staff fionr
the
Department of Physiology
and
of
Reproduction
and Faculty of
Veterinary
Medicine, Agricultural University
and of
Biology, Faculty
of Science and Mathematics,
University. No.: 0
1 1
PRAKATA
Untuk kan keane
ekosistem, jenis
k
satwa
jenis
satwa
langka,
per
konservasi bai
k
secara maupun
konservasi konservasi
yang
oleh iptek
terapan Salah satu
ptek
terapan
bidang
reproduksi yai tu pembekuan semen, dan
induksi telah
dapat dipakai
dan nutfah
Teknologi reproduksi terapan j
uga dimanfaatkan alat
atau dalam
konservasi dan satwa ayam
yang
terancam
hasi itian
tahun 20001200 data
satu
terbaik optimal
semen ayam
tahun
penelitian dilakukan
upaya meningkatkan
semen
yang
dan
tahun is
bank
gen
pembekuan semen ayam yang
disimpan
nitrogen
cair
hersuhu
dengan telah penelitian
di
tahun ini
diucapkan kasi
h
kepada Proyek
Peningkatan dan
Pada Pendidikan
LPB.
Ucapan
kasi
h
disampai kan pula
kepada
Kedokteran Ketua
Ketua
dan
yang telah
penelitian mendukung pengembangan iptek
staf yang
dapat dilupakan, kepada
Saudara bin Mopun
dan Saudara
Bernard S., yang
ah semen, diucapkan
terima kasih. Demikian pula kepada teknisi
Bagian Reproduksi
Kebidanan
Saudara yang
telah pembekuan
semen, staf
ayam Fisiologi
tim
mengucapkan kasih.
dukungan dan
ke
semua pihak, sulit
penelitian
ini dapat
