III. MATERIALS AND METHODS
3.1 Rearing Condition
The experiment was conducted at Balai Riset Budidaya Ikan Hias BRBIH Depok, West Java with the support of Institut de Recherche pour le
Développement. Fishes
were 3-inch
patin juvenile
Pangasianodon hypophthalmus with an average mass 6.4±0.2 g. Density was 12 fishes per 60 L
for each aquarium 50 cm x 40 cm x 40 cm. These fishes were obtained from Balai Besar Pengembangan Budidaya Air Tawar BBPBAT Sukabumi, West
Java. Before rearing, patin juvenile were being adapted to low fish meal diet
during one week. The experimental period lasted 6 weeks with various daily
protein supplies. Daily ration were given in the 3 equal meals at 08.00, 12.00 and 16.00. Aquariums were cleaned by siphoning every morning before first meal
07.00 am. Fish biomass was recorded every week to adjust daily ration in each aquarium. Prior to sampling, fishes were anesthetized with 600 µL L
-1
eugenol solution 10 in ethanol.
Water quality ammonia-N, pH, O
2
, temperature and conductivity was monitored weekly.
3.2 Diets
Feeds were prepared using fish meal Chile, soybean meal India, corn meal local market, wheat gluten meal Australia, palm oil local grocery, corn
oil, fish oil Aphytec, France, vitamin, mineral Agrobase
®
, France and binder Carboxyl Methyl Cellulose. The formulations were given in below Table 3.
3.3 Chemical Analysis
Chemical analyses were conducted to measure proximate composition of feed ingredients, feeds, fish body and faeces. Proximate of feed ingredients was used as
basis to formulate the diet and so proximate of diet after pelleting to check the composition. Proximate analysis of fish body was conducted at the beginning and
13 end of experimental period to measure protein and fat retention level. Proximate
of faeces were conducted to measure total of feed digestibility. Table 3. Diets formulation
Ingredients Composition g kg
-1
dry matter Diet HFM
Diet LFM Fish meal 76.4
131 24
Soybean meal 51.6 655
194 Corn meal 13.4
94 328
Wheat gluten meal 86.1 334
Palm oil 31
30 Corn oil
30 30
Fish oil 10
10 Vitamin
10 10
Mineral 30
30 Binder
10 10
Proximate composition g kg
-1
dry matter Ash
10.0 5.8
Crude protein 46.8
46.3 Fat
10.0 8.9
Crude fibre 13.6
11.8
3.4 Feed Digestibility Test
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