Pengaruh Ekstrak Bawang Putih (Allium sativum)terhadap Aktivitas Proliferase Sel Limfosit Mencit (Mus musculus) sebagai Indikator Imunostimulan secara in Vitro
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C'I:/O
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PENGARU II EKSTRAI( BA WANG PUTUI (Allillm sattvllm)
TERHADAP AKTlVITAS PROLIFERASIsEL LIMFOSIT
MENCIT (Mus musculus) SEBAGAIINInKATOR
IMUNOSTiMULAN
SECARA
TN,VITRO
',;;) , , '
"" ; >
,,' ,
Ｇｾ＠
/; ,;It
SKRIPSI
Oleh :
YUNI YUPIANA
B0149608
FAKULTAS KEDOKTERAN HEWAN
INSTITUT PERTANIAN BOGOR
2000
I'ENGARUH EKSTRAK BA WANG I'UTIH (Allium.mtil'um)
TERHADAP AKTIVITAS PROLIFERASI SEL LlMFOSIT
MENCIT (Mus musculus) SEBAGAI INDIKA TOR IMUNOSTIMULAN
SECARA IN VITRO
Oleh
Yuni Yupiana
B01496087
Skripsi ini diajukan sebagai syarat untuk memperoleh ge1ar
Sarjana Kedokteran Hewan
Pada
Fakultas Kedokteran Hewan Institut Pertanian Bogor
FAKULTAS KEDOKTERAN HEWAN
INSTITUT PERTANIAN BOGOR
2000
DAFTARISI
Halaman
DAFTARISI. ................................................................................................................... .iv
DAFTARGAMBAR. ...................................................................................................... vii
DAFTAR TABEL........................................................................................................... viii
DAFTAR LAMPIRAN .... .... '" ......................................................................................... .ix
BAB I PENDAHULUAN ................................................................................................. 1
1.1. Latar Beiakang................................................................................................. 1
1.2. TujuanPenelitian............................................................................................. 3
BAB IT TINJAUAN PUSTAKA ................................................................................... 4
2.1. Botani Bawan& Putih.................................................................................... .4
2.1.1. Taksonomi ............................................................................................. 5
2.1.2. Kandungan Kimia ...................................................... '" ........................ 7
2.1.3. ManfaatBawangPutih......................................................................... 8
2.2. Sistem Imunitas ............................................................................................. 9
2.3. Imunomoduiator. .......................................................................................... 11
2.4. Limfosit.. ...................................................................................................... 13
2.4.1. Proliferasi Sel Limfosit ....................................................................... 15
IV
2.4.2. Kultur Sel Limfosit.. ........................................................................... 18
2.5. Concanavalin A ............................................................................................ 19
BAB III BAHAN DAN METODA ................................................................................. 21
3.1. Waktu dan Tempat Penelitian ...................................................................... 21
3.2. Bahan dan Alat Penelitian........................................................................... 21
3.2.1. Alat. .................................................................................................... 21
3.2.2. Bahan.................................................................................................. 22
3.3. MetodaPenelitian........................................................................................ 22
3.3.1. Persiapan Tanaman............................................................................. 22
A. Pembuatan Ekstrak ....................................................................... 22
B. Pengukuran Kadar Air .................................................................. 23
3.3 .2. Uji Aktifitas Proliferasi ....................................................................... 23
A. Pembuatan Media ......................................................................... 23
B. Isolasi Limfosit.. ........................................................................... 24
C. Pembuatan Larutan XTT-Menadion ............................................ 25
D. Penentuan Konsentrasi
Con-A
dan
Iumlah
Sel Limfosit
Optimal. ....................................................................................... 25
E. Perngujian Pengaruh Konsentrasi Bawang Putih ......................... 26
BAB IV HASIL DAN PEMBAHASAN ....................................................................... 27
4.1. Kandungan Air Bawang Putih.................................................................... 27
4.2. Proliferasi Limfosit. .................................................................................... 28
v
4.2.1. JumJah SeJ Limfosit dan Konsentrasi Con-A OptimaJ... ..................... 28
4.2.2. Pengaruh Konsentrasi Bawang Putih................................................. 29
A. Respon Limfosit. ....................... '" ................................................. 29
B. Modulasi Proliferasi Limfosit.. .................................................... .30
BAB V KESIMPULAN DAN SARAN ...................................................................... .33
5.1. Kesimpulan ............................................................................................... 33
5.2. Saran.......................................................................................................... 33
DAFTAR PUSTAKA ..................................................................................................... .34
LAMPIRAN .................................................................................................................... .37
Vi
Wati, A., H. M. Sadikin , H. Sriwidia, Endharmini. 1986. Potensi Sari Air Bawang
Putih (Allium sativum linn.) Pada Penghambatan Kenaikan Kadar Peroksida
Lipid dalam Bati Tikus yang Diracuni dengan CCl4 . Prosiding Simposium
Penelitian Bahan Obat Alami VIII : 413-416.
Weir, D. M. 1979. Handbook of Experimental Immunology. Blackwell Scientific
Publication. Oxford, London, Edinburgh, Melbourne: 23.7-23.10.
36
fb/r Ie I-I
ｾ｟＠
C'I:/O
GoA'v
PENGARU II EKSTRAI( BA WANG PUTUI (Allillm sattvllm)
TERHADAP AKTlVITAS PROLIFERASIsEL LIMFOSIT
MENCIT (Mus musculus) SEBAGAIINInKATOR
IMUNOSTiMULAN
SECARA
TN,VITRO
',;;) , , '
"" ; >
,,' ,
Ｇｾ＠
/; ,;It
SKRIPSI
Oleh :
YUNI YUPIANA
B0149608
FAKULTAS KEDOKTERAN HEWAN
INSTITUT PERTANIAN BOGOR
2000
I'ENGARUH EKSTRAK BA WANG I'UTIH (Allium.mtil'um)
TERHADAP AKTIVITAS PROLIFERASI SEL LlMFOSIT
MENCIT (Mus musculus) SEBAGAI INDIKA TOR IMUNOSTIMULAN
SECARA IN VITRO
Oleh
Yuni Yupiana
B01496087
Skripsi ini diajukan sebagai syarat untuk memperoleh ge1ar
Sarjana Kedokteran Hewan
Pada
Fakultas Kedokteran Hewan Institut Pertanian Bogor
FAKULTAS KEDOKTERAN HEWAN
INSTITUT PERTANIAN BOGOR
2000
DAFTARISI
Halaman
DAFTARISI. ................................................................................................................... .iv
DAFTARGAMBAR. ...................................................................................................... vii
DAFTAR TABEL........................................................................................................... viii
DAFTAR LAMPIRAN .... .... '" ......................................................................................... .ix
BAB I PENDAHULUAN ................................................................................................. 1
1.1. Latar Beiakang................................................................................................. 1
1.2. TujuanPenelitian............................................................................................. 3
BAB IT TINJAUAN PUSTAKA ................................................................................... 4
2.1. Botani Bawan& Putih.................................................................................... .4
2.1.1. Taksonomi ............................................................................................. 5
2.1.2. Kandungan Kimia ...................................................... '" ........................ 7
2.1.3. ManfaatBawangPutih......................................................................... 8
2.2. Sistem Imunitas ............................................................................................. 9
2.3. Imunomoduiator. .......................................................................................... 11
2.4. Limfosit.. ...................................................................................................... 13
2.4.1. Proliferasi Sel Limfosit ....................................................................... 15
IV
2.4.2. Kultur Sel Limfosit.. ........................................................................... 18
2.5. Concanavalin A ............................................................................................ 19
BAB III BAHAN DAN METODA ................................................................................. 21
3.1. Waktu dan Tempat Penelitian ...................................................................... 21
3.2. Bahan dan Alat Penelitian........................................................................... 21
3.2.1. Alat. .................................................................................................... 21
3.2.2. Bahan.................................................................................................. 22
3.3. MetodaPenelitian........................................................................................ 22
3.3.1. Persiapan Tanaman............................................................................. 22
A. Pembuatan Ekstrak ....................................................................... 22
B. Pengukuran Kadar Air .................................................................. 23
3.3 .2. Uji Aktifitas Proliferasi ....................................................................... 23
A. Pembuatan Media ......................................................................... 23
B. Isolasi Limfosit.. ........................................................................... 24
C. Pembuatan Larutan XTT-Menadion ............................................ 25
D. Penentuan Konsentrasi
Con-A
dan
Iumlah
Sel Limfosit
Optimal. ....................................................................................... 25
E. Perngujian Pengaruh Konsentrasi Bawang Putih ......................... 26
BAB IV HASIL DAN PEMBAHASAN ....................................................................... 27
4.1. Kandungan Air Bawang Putih.................................................................... 27
4.2. Proliferasi Limfosit. .................................................................................... 28
v
ｾ｟＠
C'I:/O
GoA'v
PENGARU II EKSTRAI( BA WANG PUTUI (Allillm sattvllm)
TERHADAP AKTlVITAS PROLIFERASIsEL LIMFOSIT
MENCIT (Mus musculus) SEBAGAIINInKATOR
IMUNOSTiMULAN
SECARA
TN,VITRO
',;;) , , '
"" ; >
,,' ,
Ｇｾ＠
/; ,;It
SKRIPSI
Oleh :
YUNI YUPIANA
B0149608
FAKULTAS KEDOKTERAN HEWAN
INSTITUT PERTANIAN BOGOR
2000
I'ENGARUH EKSTRAK BA WANG I'UTIH (Allium.mtil'um)
TERHADAP AKTIVITAS PROLIFERASI SEL LlMFOSIT
MENCIT (Mus musculus) SEBAGAI INDIKA TOR IMUNOSTIMULAN
SECARA IN VITRO
Oleh
Yuni Yupiana
B01496087
Skripsi ini diajukan sebagai syarat untuk memperoleh ge1ar
Sarjana Kedokteran Hewan
Pada
Fakultas Kedokteran Hewan Institut Pertanian Bogor
FAKULTAS KEDOKTERAN HEWAN
INSTITUT PERTANIAN BOGOR
2000
DAFTARISI
Halaman
DAFTARISI. ................................................................................................................... .iv
DAFTARGAMBAR. ...................................................................................................... vii
DAFTAR TABEL........................................................................................................... viii
DAFTAR LAMPIRAN .... .... '" ......................................................................................... .ix
BAB I PENDAHULUAN ................................................................................................. 1
1.1. Latar Beiakang................................................................................................. 1
1.2. TujuanPenelitian............................................................................................. 3
BAB IT TINJAUAN PUSTAKA ................................................................................... 4
2.1. Botani Bawan& Putih.................................................................................... .4
2.1.1. Taksonomi ............................................................................................. 5
2.1.2. Kandungan Kimia ...................................................... '" ........................ 7
2.1.3. ManfaatBawangPutih......................................................................... 8
2.2. Sistem Imunitas ............................................................................................. 9
2.3. Imunomoduiator. .......................................................................................... 11
2.4. Limfosit.. ...................................................................................................... 13
2.4.1. Proliferasi Sel Limfosit ....................................................................... 15
IV
2.4.2. Kultur Sel Limfosit.. ........................................................................... 18
2.5. Concanavalin A ............................................................................................ 19
BAB III BAHAN DAN METODA ................................................................................. 21
3.1. Waktu dan Tempat Penelitian ...................................................................... 21
3.2. Bahan dan Alat Penelitian........................................................................... 21
3.2.1. Alat. .................................................................................................... 21
3.2.2. Bahan.................................................................................................. 22
3.3. MetodaPenelitian........................................................................................ 22
3.3.1. Persiapan Tanaman............................................................................. 22
A. Pembuatan Ekstrak ....................................................................... 22
B. Pengukuran Kadar Air .................................................................. 23
3.3 .2. Uji Aktifitas Proliferasi ....................................................................... 23
A. Pembuatan Media ......................................................................... 23
B. Isolasi Limfosit.. ........................................................................... 24
C. Pembuatan Larutan XTT-Menadion ............................................ 25
D. Penentuan Konsentrasi
Con-A
dan
Iumlah
Sel Limfosit
Optimal. ....................................................................................... 25
E. Perngujian Pengaruh Konsentrasi Bawang Putih ......................... 26
BAB IV HASIL DAN PEMBAHASAN ....................................................................... 27
4.1. Kandungan Air Bawang Putih.................................................................... 27
4.2. Proliferasi Limfosit. .................................................................................... 28
v
4.2.1. JumJah SeJ Limfosit dan Konsentrasi Con-A OptimaJ... ..................... 28
4.2.2. Pengaruh Konsentrasi Bawang Putih................................................. 29
A. Respon Limfosit. ....................... '" ................................................. 29
B. Modulasi Proliferasi Limfosit.. .................................................... .30
BAB V KESIMPULAN DAN SARAN ...................................................................... .33
5.1. Kesimpulan ............................................................................................... 33
5.2. Saran.......................................................................................................... 33
DAFTAR PUSTAKA ..................................................................................................... .34
LAMPIRAN .................................................................................................................... .37
Vi
Wati, A., H. M. Sadikin , H. Sriwidia, Endharmini. 1986. Potensi Sari Air Bawang
Putih (Allium sativum linn.) Pada Penghambatan Kenaikan Kadar Peroksida
Lipid dalam Bati Tikus yang Diracuni dengan CCl4 . Prosiding Simposium
Penelitian Bahan Obat Alami VIII : 413-416.
Weir, D. M. 1979. Handbook of Experimental Immunology. Blackwell Scientific
Publication. Oxford, London, Edinburgh, Melbourne: 23.7-23.10.
36
fb/r Ie I-I
ｾ｟＠
C'I:/O
GoA'v
PENGARU II EKSTRAI( BA WANG PUTUI (Allillm sattvllm)
TERHADAP AKTlVITAS PROLIFERASIsEL LIMFOSIT
MENCIT (Mus musculus) SEBAGAIINInKATOR
IMUNOSTiMULAN
SECARA
TN,VITRO
',;;) , , '
"" ; >
,,' ,
Ｇｾ＠
/; ,;It
SKRIPSI
Oleh :
YUNI YUPIANA
B0149608
FAKULTAS KEDOKTERAN HEWAN
INSTITUT PERTANIAN BOGOR
2000
I'ENGARUH EKSTRAK BA WANG I'UTIH (Allium.mtil'um)
TERHADAP AKTIVITAS PROLIFERASI SEL LlMFOSIT
MENCIT (Mus musculus) SEBAGAI INDIKA TOR IMUNOSTIMULAN
SECARA IN VITRO
Oleh
Yuni Yupiana
B01496087
Skripsi ini diajukan sebagai syarat untuk memperoleh ge1ar
Sarjana Kedokteran Hewan
Pada
Fakultas Kedokteran Hewan Institut Pertanian Bogor
FAKULTAS KEDOKTERAN HEWAN
INSTITUT PERTANIAN BOGOR
2000
DAFTARISI
Halaman
DAFTARISI. ................................................................................................................... .iv
DAFTARGAMBAR. ...................................................................................................... vii
DAFTAR TABEL........................................................................................................... viii
DAFTAR LAMPIRAN .... .... '" ......................................................................................... .ix
BAB I PENDAHULUAN ................................................................................................. 1
1.1. Latar Beiakang................................................................................................. 1
1.2. TujuanPenelitian............................................................................................. 3
BAB IT TINJAUAN PUSTAKA ................................................................................... 4
2.1. Botani Bawan& Putih.................................................................................... .4
2.1.1. Taksonomi ............................................................................................. 5
2.1.2. Kandungan Kimia ...................................................... '" ........................ 7
2.1.3. ManfaatBawangPutih......................................................................... 8
2.2. Sistem Imunitas ............................................................................................. 9
2.3. Imunomoduiator. .......................................................................................... 11
2.4. Limfosit.. ...................................................................................................... 13
2.4.1. Proliferasi Sel Limfosit ....................................................................... 15
IV
2.4.2. Kultur Sel Limfosit.. ........................................................................... 18
2.5. Concanavalin A ............................................................................................ 19
BAB III BAHAN DAN METODA ................................................................................. 21
3.1. Waktu dan Tempat Penelitian ...................................................................... 21
3.2. Bahan dan Alat Penelitian........................................................................... 21
3.2.1. Alat. .................................................................................................... 21
3.2.2. Bahan.................................................................................................. 22
3.3. MetodaPenelitian........................................................................................ 22
3.3.1. Persiapan Tanaman............................................................................. 22
A. Pembuatan Ekstrak ....................................................................... 22
B. Pengukuran Kadar Air .................................................................. 23
3.3 .2. Uji Aktifitas Proliferasi ....................................................................... 23
A. Pembuatan Media ......................................................................... 23
B. Isolasi Limfosit.. ........................................................................... 24
C. Pembuatan Larutan XTT-Menadion ............................................ 25
D. Penentuan Konsentrasi
Con-A
dan
Iumlah
Sel Limfosit
Optimal. ....................................................................................... 25
E. Perngujian Pengaruh Konsentrasi Bawang Putih ......................... 26
BAB IV HASIL DAN PEMBAHASAN ....................................................................... 27
4.1. Kandungan Air Bawang Putih.................................................................... 27
4.2. Proliferasi Limfosit. .................................................................................... 28
v