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Karakteristik Oosit Domba dari Ovarium yang Disimpan pada Suhu dan Periode Waktu yang Berbeda

ABSTRACT

DHIA MARDHIA ENGCONG. Characteristic of Sheep Oocytes from the
Ovaries that was Store on Different Temperature and Different Period Of Time.
Under direction of NI WAYAN KURNIANI KARJA.
The aim of the study was to investigate the characteristic of sheep oocytes
from the ovaries that was stored on different temperature at a different period of
time. Ovaries obtained from the slaughter house. The ovaries were then stored at
4 ˚C for the period of 2, 5-7 and 8-10 h, 27-28 ˚C for the 2, 5-7 and 8-10 h, and
the third group was stored at 37-38 ˚C for 2, 5-7 and 8-10 h. At the end of the
storage, the oocytes were collected from the ovaries using the slicing method.
Collected oocytes were graded as A, B, C and D based on the layers of the
cumulus cell and images of the cytoplasm. The percentage of the oocytes for
grade A, B, C and D that was derived from the ovaries stored at 4 ˚C are no differ
than the other group (P>0.05). The percentage of oocytes of A’s grade from the
ovaries stored at the temperature of 27-28 ˚C for 2 hours were higher than the
oocytes from the ovaries that were stored for 5-7 and 8-10 hours (P>0.05).
Meanwhile the quantity of oocytes with the grade of B, C and D that was derived
from the ovaries stored at the temperature of 27-28 ˚C did have not change
compared to the other group (P>0.05). The quantity of oocytes with A’s grade that
was derived from the ovaries that was kept at the temperature 37-38 ˚C for 2

hours and 5-7 hours (P

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