KELARUTAN OIL SLUDGE DENGAN BIOSURFAKTAN Acinetobacter sp. P2(1) DAN VARIASI VOLUME CRUDE ENZIM LIPASE Bacillus sp. LII63B Repository - UNAIR REPOSITORY

  Lampiran 1. Data tegangan permukaan supernatan produksi biosurfaktan Acinetobacter sp. P2(1) (dyne/cm)

  Kode TP 1 TP2 TP3 Rata2 Sd r TP (dyne/cm) P1B1 74,2 79,6 83,3 79,03 4,57 54,80

  P1B2 80,5 83,2 82,8 82,17 1,46 56,97 P1B3 72,2 75,6 76,4 74,73 2,23 51,82 P1B4 72,4 77 80,3 76,57 3,97 53,09 P1B5 76,8 80,7 84,3 80,60 3,75 55,88 P1B6 76,4 80 82,3 79,57 2,97 55,17 P1B7 83,9

  83 82,8 83,23 0,58 57,71 P1B8 88 87,3 86,4 87,23 0,80 60,48 P1B11 75,6 79,7

  82 79,1 3,24 54,84 P1B13 73,2 76,4 79 76,2 2,90 52,83 P1B15 80,4 83,5 87,3 83,73 3,45 58,06

  P1B17 78,7 79,5 81,9 80,03 1,66 55,49 P1B19 79,1 80 80,9 80 0,9 55,47 P1B21 79,2 81,5 82,5 81,07 1,69 56,21 akuades 102,6 104,5 104,4 103,83 1,06

  72 kontrol 78,8 79,1 78,9 78,93 0,15 54,73 P2B2 68 69,6 73,1 70,23 2,61 48,36 P2B3

  67 72,8 73,9 71,23 3,71 49,04 P2B4 69 74,5 76 73,17 3,68 50,37 P2B5 70 72,9 75,4 72,77 2,70 50,10 akuades 104,4 104,7 104,6 104,57 0,15

  72 kontrol 72,9 72,8 73,1 72,93 0,15 50,22 Rata-Rata Tegangan permukaan 54,26 Rata-rata standart deviasi 3,32

  

ADLN Perpustakaan Universitas Airlangga Lampiran 2. Data nilai emulsifikasi supernatan produksi biosurfaktan Acinetobacter sp. P2(1) (%) pada minyak uji solar

  Kode % 1 Jam % 24 Jam

  2 19,2

  3 1,31 2,76 14,11 10,65

  13,8

  2 9,32 8,8

  1 12,8 15,4

  9 14,1

  P1B1

  4 8,36 4,08 12,9 7,63 4,18 4,43 8,36 8,20

  7 4,18 12,5

  P1B1

  1 1,25 1,94 24,44 21,4

  7 22,9

  1 11,3

  9 22,0

  9 23,1

  4 25,6

  5 24,4

  P1B1

  5 15,88 7,39 28,18 21,24

  4 13,2

  3 27,8

  6 22,6

  6 15,3

  3 45,9

  P1B2

  5 10,6

  P1B1

  2 26,8

  Rata-Rata 4,79 4,39 21,19 17,66

  6 5,78 7,12 27,40 26,06

  8 32,2

  3 18,2

  3 27,6

  8 31,4

  30 20,7

  P2B5

  2 35,6 7,79 8,92 37,09 35,73

  7 44,7

  5 45,8 34,7

  7 6,25 9,09 8,87 5,1 2,76 2,24 9,42 7,68

  P2B4 30,7

  8 24,6 8,92 9,09 35,17 31,54

  4 28,1

  5 41,8

  6 28,4

  P2B3 45,3 31,7

  6 10,51 10,49 19,27 16,76

  5 19,8

  2 5,06 25,3

  7 21,2

  P2B2 7,92 28,6

  3 23,2

  6 3,13 4,97 4,14 10,47 7,82

  Sd % 1 Jam

  5 5,43 17,4

  1 54,5

  2 54,8

  2 58,4

  4 60,5

  P1B3 62,5

  1 15,4 9,23 11,2 2,26 3,15 16,21 11,94

  2 17,8

  1 13,6

  P1B2 17,2

  9 5,92 7,62 19,49 14,16

  9 19,5

  3 2,06 0,29 60,49 54,52

  7 19,4

  1 13,5

  3 P1B1 25,4

  2 AE

  1 AE

  3 AE

  2 AE

  1 AE

  AE

  Jam Rata2 % 24 jam

  Sd % 24 jam Rata2 % 1

  3 54,2

  P1B4 2,94 11,2

  3 4,88 9,38 10,9

  5 16,3

  1 12,1

  1 14,4

  P1B1

  1 8,36 6,1 3,19 2,25 10,82 8,36

  3 7,63 10,6

  2 10,8

  P1B8 14,0

  1 1,60 1,57 15,57 14,78

  8 13,2

  5 14,7

  4 14,1

  9 7,12 1,25 9,68 5,22 4,17 4,21 7,12 5,38

  1 15,2

  P1B7 17,3

  3 4,7 3,82 6,23 1,71 1,21 9,62 4,92

  P1B6 9,8 7,82 11,2

  5 1,99 0,170 28,26 16,91

  9 16,7

  9 17,0

  3 16,8

  2 26,2

  3 30,2

  P1B5 28,3

  

ADLN Perpustakaan Universitas Airlangga Lampiran 3. Grafik karakteristik biosurfaktan (tegangan permukaan, aktivitas emulsifikasi, dan berat kering produk kasar) dalam supernatan kultur Acinetobacter sp. P2(1)

  

ADLN Perpustakaan Universitas Airlangga

  

ADLN Perpustakaan Universitas Airlangga

Lampiran 4. Nilai tegangan permukaan produk kasar biosurfaktan Acinetobacter sp. P2(1) dan tween-20 dengan konsentrasi sama dengan CMC (dyne/cm)

  Standart TP Rata- r TP Perlakuan deviasi (dyne/cm) rata (dyne/cm)

  (±) 129,8

Akuades 128 129,77

72 1,75 131,5

  126,6 Buffer fosfat pH 7 128,2 127,87 70,95 1,13 128,8 96,6

  Produk kasar biosurfaktan 97,2 96,87 53,74 0,31 (=CMC) 96,8

  101,8 Tween-20 (=CMC) 102,3 102,3 56,76 0,5 102,8 Lampiran 5. Nilai aktivitas emulsifikasi produk kasar biosurfaktan Acinetobacter sp. P2(1) dan tween-20 dengan konsentrasi sama dengan CMC (%) pada minyak uji solar Perlakuan

  Persentase kelarutan (%) 1 jam 24 jam Biosurfaktan 42,59 40,34

  42,86 40,75 38,1 36,69 Rata-rata 41,18 ± 2,68 39,26 ± 2,24 Tween-20

  34,72 32,65 36,67 43,21 35,1 29,99 Rata-rata 35,5 ± 1,031 32,25 ± 2,19

  

ADLN Perpustakaan Universitas Airlangga

  

ADLN Perpustakaan Universitas Airlangga

Lampiran 6. Nilai serapan crude enzim lipase Bacillus sp. LII63B dengan UV-Vis dengan nilai λ= 410 pada waktu 16 jam

  Waktu inkubasi Nilai serapan UV-Vis 1 0,129 16 jam 2 0,127 Rata-rata 0,129 ± 0,001

  Kemudian nilai serapan yang didapatkan dimasukkan ke dalam kurva standart p-nitrofenol untuk mengetahui nilai aktivitas enzimnya, Gambar, Kurva standart p-nitrofenol

  

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Lampiran 7. Nilai blanko kelarutan oil sludge (%) pada masing-masing perlakuan Perlakuan Wo Wt Wb Wb X 8

  

K 1,037 1,0399 0,0002 0,0016

S 1,1203 1,1251 0,0048 0,0384

B 1,146 1,1509 0,0049 0,0392

E1 1,1001 1,1073 0,0072 0,0576

  

E2 1,136 1,1394 0,0034 0,0272

E3 1,1842 1,1875 0,0033 0,0264

BE1 1,1698 1,1719 0,0021 0,0168

BE2 1,2067 1,2117 0,005 0,04

BE3 1,1844 1,1893 0,0049 0,0392

  • perlakuan blanko tidak disertai dengan penambahan oil sludge

  

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Lampiran 8. Nilai kelarutan oil sludge (%) pada masing-masing perlakuan

Keterangan : K : penambahan akuades pada oil sludge,

  

S : penambahan surfaktan sintetis (tween-20) pada oil sludge.

B : penambahan biosurfaktan Acinetobacter sp. P2(1) pada oil sludge. E1 : penambahan 1 mL crude enzim Bacillus sp. LII63B pada oil sludge. E2 : penambahan 2 mL crude enzim Bacillus sp. LII63B pada oil sludge. E3 : penambahan 3 mL crude enzim Bacillus sp. LII63B pada oil sludge. BE1 : penambahan biosurfaktan dan 1 mL crude enzim Bacillus sp. LII63B pada oil sludge.

  BE2 : penambahan biosurfaktan dan 2 mL crude enzim Bacillus sp. LII63B pada oil sludge. BE3 : penambahan biosurfaktan dan 3 mL crude enzim Bacillus sp. LII63B pada oil sludge. Lampiran 9. Rekapan hasil analisis signifikansi masing-masing perlakuan berdasarkan pada uji Independent Samples T-Test K S B E1 E2 E3 BE1 BE2 BE3 K S S S S S S S S S S S S S S TS TS B S S S TS S S E1 S S S S S E2

  S S S TS E3 S S TS BE1

  TS S BE2 TS BE3

  Keterangan : S : signifikan TS : tidak signifikan

  

ADLN Perpustakaan Universitas Airlangga

  

ADLN Perpustakaan Universitas Airlangga

Lampiran 10. Analisis statistik masing-masing perlakuan pada kelarutan oil sludge

  NPar Tests One-Sample Kolmogorov-Smirnov Test Hasil N

  27 a,,b

  Normal Parameters Mean 30.0554 Std. Deviation 14.32734 Most Extreme Absolute .117 Differences

  Positive .090 Negative -.117 Kolmogorov-Smirnov Z .607 Asymp. Sig. (2-tailed) .854 a. Test distribution is Normal.

  b. Calculated from data.

  Test of Homogeneity of Variances Hasil Levene

  Statistic df1 df2 Sig.

  3.025

  8 18 .024 Robust Tests of Equality of Means

  Hasil a Statistic df1 df2 Sig.

  Brown- 142.889 8 8.643 .000 Forsythe a. Asymptotically F distributed. H : Tidak ada pengaruh yang signifikan

1 H : Ada pengaruh yang signifikan

  α = 0,05 (P value < α) Kesimpulan : Tolak H

  

ADLN Perpustakaan Universitas Airlangga

Lampiran 11. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Akuades dengan tween-20; (2) Akuades dengan biosurfaktan Acinetobacter sp. P2(1)

  T-Test 1. Group Statistics Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K

  3 3.5928 .23952 .13829 S 3 33.8520 1.53972 .88896

  Independent Samples Test Levene's Test for

  Equality of Variances t-test for Equality of Means 95% Confidence Interval of the

  Sig.

  Difference (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper Hasil Equal 9.190 .039 4 .000 -30.25918 .89965 -32.75701 -27.76136 - variances 33.634 assumed

  • Equal 2.097 .001 -30.25918 .89965 -33.96385 -26.55452 variances 33.634 not assumed

  T-Test 2. Group Statistics Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K

  3 3.5928 .23952 .13829 B 3 22.0359 2.87425 1.65945

  Independent Samples Test

  Levene's Test for Equality of

  Variances t-test for Equality of Means 95% Confidence

  Interval of the Difference

  Sig. (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper

  • Hasil Equal variances 3.338 .142 4 .000 -18.44311 1.66520 - - assumed 11.076 23.06646 13.81977
  • Equal variances 2.028 .008 -18.44311 1.66520 - - not assumed 11.076 25.51467 11.37156

  

ADLN Perpustakaan Universitas Airlangga

Lampiran 12. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Akuades dengan crude enzim lipase 12,5% (v/v);

  (2) Akuades dengan crude enzim lipase 25% (v/v)

  1. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K 3 3.5928 .23952 .13829 E1 3 12.6946 2.15565 1.24457 Independent Samples Test

  Levene's Test for Equality of Variances t-test for Equality of Means 95% Confidence

  Interval of the Sig.

  Difference (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper

  • Hasil Equal 3.122 .152 4 .002 -9.10183 1.25223 variances 7.269 12.57857 5.62509 assumed
  • Equal - 2.049 .017 -9.10183 1.25223 - variances not 7.269 14.36722 3.83644 assumed

  2. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K 3 3.5928 .23952 .13829 E2 3 40.9581 .23952 .13829 Independent Samples Test

  Levene's Test for Equality of Variances t-test for Equality of Means 95% Confidence

  Interval of the Sig.

  Difference (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper

  • Hasil Equal .000 1.000 4 .000 -37.36527 .19557 variances 191.060 37.90825 36.82229 assumed
  • Equal 4.000 .000 -37.36527 .19557 variances 191.060 37.90825 36.82229 not assumed

  • 39.69654 Equal variances not assumed
  • 39.69654
  • 17.64303
  • 12.669
  • 15.00826

  • 205.757 4.000 .000 -40.23952 .19557 - 40.78250

  2.024 .006 -22.59481 1.78350 - 30.18136

  Equal variances not assumed

  27.54659

  11.188 .029 - 12.669 4 .000 -22.59481 1.78350 -

  Lower Upper Hasil Equal variances assumed

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Independent Samples Test

  205.757 4 .000 -40.23952 .19557 - 40.78250

  Difference Lower Upper Hasil Equal variances assumed .000 1.000 -

  Std. Error Difference 95% Confidence Interval of the

  (2- tailed) Mean Difference

  Sig.

  Levene's Test for Equality of Variances t-test for Equality of Means F Sig. t df

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K 3 3.5928 .23952 .13829 BE1 3 26.1876 3.07981 1.77813 Independent Samples Test

  2. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K 3 3.5928 .23952 .13829 E3 3 43.8323 .23952 .13829

  1. T-Test

Group Statistics

  Lampiran 13. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Akuades dengan crude enzim lipase 37,5% (v/v); (2) Akuades dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 12,5% (v/v)

  

ADLN Perpustakaan Universitas Airlangga

  

ADLN Perpustakaan Universitas Airlangga

  33.31093

  2.002 .018 -43.03393 5.91627 - 68.46297

  Equal variances not assumed

  59.46013

  15.037 .018 - 7.274 4 .002 -43.03393 5.91627 -

  Lower Upper Hasil Equal variances assumed

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Independent Samples Test

  2.023 .004 -28.18363 1.84671 - 36.04508

  Equal variances not assumed

  7.269 .054 - 15.262 4 .000 -28.18363 1.84671 -

  Independent Samples Test

  BE2 3 31.7764 3.18962 1.84153

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Lower Upper Hasil Equal variances assumed

  Lampiran 14. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Akuades dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 25% (v/v); (2) Akuades dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v)

  1. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K 3 3.5928 .23952 .13829

  • 23.05634
  • 15.262
  • 20.32219

  2. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K 3 3.5928 .23952 .13829

  BE3 3 46.6267 10.24448 5.91465

  • 26.60773
  • 7.274
  • 17.60490

  

ADLN Perpustakaan Universitas Airlangga

Lampiran 15. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Tween-20 dengan biosurfaktan Acintobacter sp.P2(1); (2) Tween-20 dengan crude enzim lipase 12,5% (v/v)

  1. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil S 3 33.8520 1.53972 .88896

  B 3 22.0359 2.87425 1.65945

  Independent Samples Test

  Levene's Test for Equality of

  Variances t-test for Equality of Means 95% Confidence

  Interval of the Difference

  Sig. (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper

  Hasil Equal variances .543 .502 6.277 4 .003 11.81607 1.88256 6.58925 17.04288 assumed Equal variances 6.277 3.061 .008 11.81607 1.88256 5.89142 17.74072 not assumed

  2. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil S 3 33.8520 1.53972 .88896 E1 3 12.6946 2.15565 1.24457

  Independent Samples Test Levene's Test for Equality of

  Variances t-test for Equality of Means 95% Confidence Interval of the Difference

  Sig. (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper Hasil Equal variances .114 .752 13.833 4 .000 21.15735 1.52944 16.91094 25.40376 assumed

  Equal variances 13.833 3.619 .000 21.15735 1.52944 16.72883 25.58587 not assumed

  

ADLN Perpustakaan Universitas Airlangga

Lampiran 16. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Tween-20 dengan crude enzim lipase 25% (v/v);

  (2) Tween-20 dengan crude enzim lipase 37,5% (v/v)

  1. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil S 3 33.8520 1.53972 .88896 E2 3 40.9581 .23952 .13829

  Independent Samples Test Levene's Test for Equality of Variances t-test for Equality of Means

  95% Confidence Interval of the Difference Sig. (2- Mean Std. Error

  F Sig. t df tailed) Difference Difference Lower Upper Hasil Equal variances 9.190 .039 -7.899 4 .001 -7.10609 .89965 -9.60391 -4.60826 assumed

  Equal variances -7.899 2.097 .014 -7.10609 .89965 -10.81075 -3.40142 not assumed

  2. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil S 3 33.8520 1.53972 .88896

  E3 3 43.8323 .23952 .13829

  Independent Samples Test

  Levene's Test for Equality of

  Variances t-test for Equality of Means 95% Confidence

  Interval of the Difference

  Sig. (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper

  • Hasil Equal variances 9.190 .039 4 .000 -9.98034 .89965 - -7.48252 assumed 11.094 12.47816

  2.097 - Equal variances .007 -9.98034 .89965 -6.27568 - not assumed 11.094 13.68500

  

ADLN Perpustakaan Universitas Airlangga

Lampiran 17. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Tween-20 dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 12,5% (v/v); (2) Tween-20 dengan biosurfaktan 37,5% (v/v) crude enzim lipase 25% (v/v)

  1. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil S 3 33.8520 1.53972 .88896 BE1 3 26.1876 3.07981 1.77813

  Independent Samples Test Levene's Test for Equality of Variances t-test for Equality of Means

  95% Confidence Interval of the Difference Sig. (2- Mean Std. Error

  F Sig. t df tailed) Difference Difference Lower Upper Hasil Equal variances 2.617 .181 3.855 4 .018 7.66437 1.98796 2.14491 13.18383 assumed

  Equal variances 3.855 2.941 .032 7.66437 1.98796 1.26535 14.06339 not assumed

  2. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil S 3 33.8520 1.53972 .88896

  BE2 3 31.7764 3.18962 1.84153

  Independent Samples Test

  Levene's Test for Equality of

  Variances t-test for Equality of Means 95% Confidence

  Interval of the Difference

  Sig. (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper

  Hasil Equal variances 1.820 .249 1.015 4 .368 2.07555 2.04486 -3.60190 7.75300 assumed Equal variances 1.015 2.884 .388 2.07555 2.04486 -4.58260 8.73369 not assumed

  

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  29.38090 3.83140

  4.503 3.709 .013 9.34128 2.07430 3.39957 15.28300

  .160 .710 4.503 4 .011 9.34128 2.07430 3.58210 15.10046 Equal variances not assumed

  Lower Upper Hasil Equal variances assumed

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Independent Samples Test

  11.92312

  2.090 .161 -12.77475 5.98108 - 37.47263

  Equal variances not assumed

  11.079 .029 - 2.136 4 .100 -12.77475 5.98108 -

  Lampiran 18. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Tween-20 dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v); (2) Biosurfaktan Acinetobacter sp.P2(1) dengan crude enzim lipase 12,5% (v/v)

  Lower Upper Hasil Equal variances assumed

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Independent Samples Test

  E1 3 12.6946 2.15565 1.24457

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil B 3 22.0359 2.87425 1.65945

  2. T-Test

Group Statistics

  BE3 3 46.6267 10.24448 5.91465

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil S 3 33.8520 1.53972 .88896

  1. T-Test

Group Statistics

  • 2.136

  

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  23.54550

  2.028 .005 -21.79641 1.66520 - 28.86796

  Equal variances not assumed

  26.41975

  3.338 .142 - 13.089 4 .000 -21.79641 1.66520 -

  Lower Upper Hasil Equal variances assumed

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Independent Samples Test

  2.028 .007 -18.92216 1.66520 - 25.99371

  Equal variances not assumed

  3.338 .142 - 11.363 4 .000 -18.92216 1.66520 -

  Independent Samples Test

  E2 3 40.9581 .23952 .13829

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Lower Upper Hasil Equal variances assumed

  Lampiran 19. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Biosurfaktan Acinetobacter sp.P2(1) dengan crude enzim lipase 25% (v/v); (2) Biosurfaktan Acinetobacter sp.P2(1) dengan crude enzim lipase 37,5% (v/v)

  1. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil B 3 22.0359 2.87425 1.65945

  • 14.29881
  • 11.363
  • 11.85060

  2. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil B 3 22.0359 2.87425 1.65945

  E3 3 43.8323 .23952 .13829

  • 17.17306
  • 13.089
  • 14.72485

  

ADLN Perpustakaan Universitas Airlangga

  10.90452 2.60113

  3.957 .017 -9.74052 2.47891 -16.65238 -2.82865

  Equal variances not assumed

  .116 .751 - 3.929 4 .017 -9.74052 2.47891 -16.62308 -2.85796

  Lower Upper Hasil Equal variances assumed

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Independent Samples Test

  2.61381

  3.981 .163 -4.15170 2.43218 - 10.91720

  Equal variances not assumed

  .136 .731 - 1.707 4 .163 -4.15170 2.43218 -

  Independent Samples Test

  BE1 3 26.1876 3.07981 1.77813

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Lower Upper Hasil Equal variances assumed

  Lampiran 20. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Biosurfaktan Acinetobacter sp.P2(1) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 12,5% (v/v); (2) Biosurfaktan Acinetobacter sp.P2(1) dengan biosurfaktan 37,5% (v/v) crude enzim lipase 25% (v/v)

  1. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil B 3 22.0359 2.87425 1.65945

  • 1.707

  2. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil B 3 22.0359 2.87425 1.65945

  BE2 3 31.7764 3.18962 1.84153

  • 3.929

  

ADLN Perpustakaan Universitas Airlangga

  7.293 .054 - 4.003 4 .016 -24.59082 6.14304 -

  2.049 .002 -28.26344 1.25223 - 33.52883

  Equal variances not assumed

  31.74018

  3.122 .152 - 22.571 4 .000 -28.26344 1.25223 -

  Lower Upper Hasil Equal variances assumed

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Independent Samples Test

  2.313 .045 -24.59082 6.14304 - 47.87440

  41.64662

  Lower Upper Hasil Equal variances assumed

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Independent Samples Test

  E2 3 40.9581 .23952 .13829

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E1 3 12.6946 2.15565 1.24457

  2. T-Test

Group Statistics

  BE3 3 46.6267 10.24448 5.91465

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil B 3 22.0359 2.87425 1.65945

  1. T-Test

Group Statistics

  Lampiran 21. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Biosurfaktan Acinetobacter sp.P2(1) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v); (2) Crude enzim lipase 12,5% (v/v) dengan crude enzim lipase 25% (v/v)

  • 7.53501 Equal variances not assumed
  • 4.003
  • 1.30723

  • 24.78670
  • 22.571
  • 22.99805

  

ADLN Perpustakaan Universitas Airlangga

Lampiran 22. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Crude enzim lipase 12,5% (v/v) dengan crude enzim lipase 37,5% (v/v); (2) Crude enzim lipase 12,5% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 12,5% (v/v)

  1. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E1 3 12.6946 2.15565 1.24457

  E3 3 43.8323 .23952 .13829

  Independent Samples Test

  Levene's Test for Equality of

  Variances t-test for Equality of Means 95% Confidence

  Interval of the Difference

  Sig. (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper

  4 - Hasil Equal variances - 3.122 .152 .000 -31.13769 1.25223 - assumed 24.866 34.61443 27.66095 Equal variances - 2.049 .001 -31.13769 1.25223 - - not assumed 24.866 36.40308 25.87230

  2. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E1 3 12.6946 2.15565 1.24457

  BE1 3 26.1876 3.07981 1.77813

  Independent Samples Test

  Levene's Test for Equality of

  Variances t-test for Equality of Means 95% Confidence

  Interval of the Difference

  Sig. (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper

  • Hasil Equal variances .875 .402 4 .003 -13.49298 2.17041 -19.51901 -7.46695 assumed 6.217
  • Equal variances 3.580 .005 -13.49298 2.17041 -19.80807 -7.17789 not assumed 6.217

  

ADLN Perpustakaan Universitas Airlangga

  25.25286

  2.177 .025 -33.93210 6.04418 - 58.01562

  Equal variances not assumed

  50.71343

  9.277 .038 - 5.614 4 .005 -33.93210 6.04418 -

  Lower Upper Hasil Equal variances assumed

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Independent Samples Test

  3.512 .002 -19.08180 2.22265 - 25.60651

  Equal variances not assumed

  .703 .449 - 8.585 4 .001 -19.08180 2.22265 -

  Independent Samples Test

  BE2 3 31.7764 3.18962 1.84153

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Lower Upper Hasil Equal variances assumed

  Lampiran 23. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Crude enzim lipase 12,5% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 25% (v/v); (2) Crude enzim lipase 12,5% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v)

  1. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E1 3 12.6946 2.15565 1.24457

  • 12.91074
  • 8.585
  • 12.55709

  2. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E1 3 12.6946 2.15565 1.24457

  BE3 3 46.6267 10.24448 5.91465

  • 17.15078
  • 5.614
  • 9.84859

  

ADLN Perpustakaan Universitas Airlangga

  .000 1.000 - 14.697 4 .000 -2.87425 .19557 -3.41724 -2.33127

  8.282 2.024 .014 14.77046 1.78350 7.18391 22.35701

  11.188 .029 8.282 4 .001 14.77046 1.78350 9.81868 19.72224 Equal variances not assumed

  Lower Upper Hasil Equal variances assumed

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Independent Samples Test

  4.000 .000 -2.87425 .19557 -3.41724 -2.33127

  Equal variances not assumed

  Lower Upper Hasil Equal variances assumed

  Lampiran 24. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Crude enzim lipase 25% (v/v) dengan crude enzim lipase 37,5% (v/v); (2) Crude enzim lipase 25% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 12,5% (v/v)

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Independent Samples Test

  BE1 3 26.1876 3.07981 1.77813

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E2 3 40.9581 .23952 .13829

  2. T-Test

Group Statistics

  E3 3 43.8323 .23952 .13829

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E2 3 40.9581 .23952 .13829

  1. T-Test

Group Statistics

  • 14.697

  

ADLN Perpustakaan Universitas Airlangga

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E2 3 40.9581 .23952 .13829

  31.09770 19.76037

  10.75754 Equal variances not assumed

  15.037 .018 -.958 4 .392 -5.66866 5.91627 - 22.09486

  Lower Upper Hasil Equal variances assumed

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Independent Samples Test

  BE3 3 46.6267 10.24448 5.91465

  2. T-Test

Group Statistics

  Lampiran 25. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Crude enzim lipase 25% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 25% (v/v); (2) Crude enzim lipase 25% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v)

  4.972 2.023 .037 9.18164 1.84671 1.32019 17.04308

  7.269 .054 4.972 4 .008 9.18164 1.84671 4.05434 14.30893 Equal variances not assumed

  Lower Upper Hasil Equal variances assumed

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Independent Samples Test

  BE2 3 31.7764 3.18962 1.84153

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E2 3 40.9581 .23952 .13829

  1. T-Test

Group Statistics

  • .958 2.002 .439 -5.66866 5.91627 -
Lampiran 26. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Crude enzim lipase 37,5% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 12,5% (v/v); (2) Crude enzim lipase 37,5% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 25% (v/v)

  1. T-Test

Group Statistics

  BE2 3 31.7764 3.18962 1.84153

  6.528 2.023 .022 12.05589 1.84671 4.19444 19.91733

  7.269 .054 6.528 4 .003 12.05589 1.84671 6.92859 17.18318 Equal variances not assumed

  Lower Upper Hasil Equal variances assumed

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Independent Samples Test

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E3 3 43.8323 .23952 .13829

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E3 3 41.8363 3.46547 2.00079

  2. T-Test

Group Statistics

  Equal variances not assumed 5.846 3.946 .004 15.64870 2.67674 8.17630 23.12111

  Hasil Equal variances assumed .116 .751 5.846 4 .004 15.64870 2.67674 8.21689 23.08051

  Std. Error Difference Lower Upper

  Mean Difference

  F Sig. t df Sig. (2- tailed)

  Interval of the Difference

  Variances t-test for Equality of Means 95% Confidence

  Levene's Test for Equality of

  Independent Samples Test

  BE1 3 26.1876 3.07981 1.77813

  

ADLN Perpustakaan Universitas Airlangga

  

ADLN Perpustakaan Universitas Airlangga

  13.63179 Equal variances not assumed

  1.52197

  3.995 .094 -5.58882 2.55988 - 12.69961

  Equal variances not assumed

  12.69618 1.51853

  .000 1.000 - 2.183 4 .094 -5.58882 2.55988 -

  Lower Upper Hasil Equal variances assumed

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Independent Samples Test

  28.22345 22.63462

  15.037 .018 -.472 4 .661 -2.79441 5.91627 - 19.22061

  Independent Samples Test

  BE3 3 46.6267 10.24448 5.91465

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Lower Upper Hasil Equal variances assumed

  Lampiran 27. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Crude enzim lipase 37,5% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v); (2) Biosurfaktan 37,5% (v/v) dan crude enzim lipase 12,5% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 25% (v/v)

  1. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E3 3 43.8323 .23952 .13829

  • .472 2.002 .683 -2.79441 5.91627 -

  2. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil BE1 3 26.1876 3.07981 1.77813

  BE2 3 31.7764 3.18962 1.84153

  • 2.183

  

ADLN Perpustakaan Universitas Airlangga

  Difference 95% Confidence

  2.61497

  Independent Samples Test

  Levene's Test for Equality of

  Variances t-test for Equality of Means F Sig. t df

  Sig. (2- tailed) Mean

  Difference Std. Error

  Interval of the Difference

  37.58687

  Lower Upper Hasil Equal variances assumed

  6.656 .061 - 2.397 4 .075 -14.85030 6.19470 -

  32.04955 2.34895

  Equal variances not assumed

  2.384 .118 -14.85030 6.19470 - 37.78341

  8.08281

  2.359 .064 -20.43912 6.17615 - 43.49321

  7.005 .057 - 3.309 4 .030 -20.43912 6.17615 -

  Independent Samples Test

  BE3 3 46.6267 10.24448 5.91465

  Interval of the Difference

  Difference 95% Confidence

  Difference Std. Error

  Sig. (2- tailed) Mean

  Variances t-test for Equality of Means F Sig. t df

  Levene's Test for Equality of

  Lower Upper Hasil Equal variances assumed

  Lampiran 28. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Biosurfaktan 37,5% (v/v) dan crude enzim lipase 12,5% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v); (2) Biosurfaktan 37,5% (v/v) dan crude enzim lipase 25% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v)

  1. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil BE1 3 26.1876 3.07981 1.77813

  • 3.29137 Equal variances not assumed
  • 3.309

  2. T-Test

Group Statistics

  Perlakuan N Mean Std. Deviation Std. Error Mean Hasil BE2 3 31.7764 3.18962 1.84153

  BE3 3 46.6267 10.24448 5.91465

  • 2.397

  

ADLN Perpustakaan Universitas Airlangga

Lampiran 29. Produksi Biosurfaktan Acinetobacter sp.P2(1)

  1

  2

  3

  4

  5 Keterangan : 1 : kultur yang diproduksi setelah 3 hari 2 : kontrol media produksi 3 : supernatan kultur setelah disentrifugasi pada saat panen 4 : hasil liofilisasi supernatan 5 : produk biosurfaktan keseluruhan

  

ADLN Perpustakaan Universitas Airlangga

Lampiran 30. Produksi Crude enzim lipase Bacillus sp. LII63B

  1

  2

  3

  4

  5

  6 Keterangan : 1 : media Bushnell Hash + 1% minyak untuk uji aktivitas enzim 2 : media NB 3 : isolat Bacillus sp. LII63B dalam media NB 4 : media produksi crude enzim lipase Bacillus sp. LII63B sebelum

5: media produksi crude enzim lipase Bacillus sp. LII63B sesudah inkubasi 6 : isolat Bacillus sp. LII63B dengan perbesaran 1000 X menggunakan mikroskop. Lampiran 31. Uji kelarutan oil sludge pada masing-masing perlakuan B

  2

  1

3 B

  3 B

ADLN Perpustakaan Universitas Airlangga

  1

  2

  1

  2

  3 S B

  1

  2

  1

  2

  3 K B

  1

  2

3 B

3 B

  B

3 B

  3 E2 B

  3 E3

ADLN Perpustakaan Universitas Airlangga

  1

  2

  1

  2

  1

  2

  2

  1

  2

  3 E1 B

  1

  2

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