KELARUTAN OIL SLUDGE DENGAN BIOSURFAKTAN Acinetobacter sp. P2(1) DAN VARIASI VOLUME CRUDE ENZIM LIPASE Bacillus sp. LII63B Repository - UNAIR REPOSITORY
Lampiran 1. Data tegangan permukaan supernatan produksi biosurfaktan Acinetobacter sp. P2(1) (dyne/cm)
Kode TP 1 TP2 TP3 Rata2 Sd r TP (dyne/cm) P1B1 74,2 79,6 83,3 79,03 4,57 54,80
P1B2 80,5 83,2 82,8 82,17 1,46 56,97 P1B3 72,2 75,6 76,4 74,73 2,23 51,82 P1B4 72,4 77 80,3 76,57 3,97 53,09 P1B5 76,8 80,7 84,3 80,60 3,75 55,88 P1B6 76,4 80 82,3 79,57 2,97 55,17 P1B7 83,9
83 82,8 83,23 0,58 57,71 P1B8 88 87,3 86,4 87,23 0,80 60,48 P1B11 75,6 79,7
82 79,1 3,24 54,84 P1B13 73,2 76,4 79 76,2 2,90 52,83 P1B15 80,4 83,5 87,3 83,73 3,45 58,06
P1B17 78,7 79,5 81,9 80,03 1,66 55,49 P1B19 79,1 80 80,9 80 0,9 55,47 P1B21 79,2 81,5 82,5 81,07 1,69 56,21 akuades 102,6 104,5 104,4 103,83 1,06
72 kontrol 78,8 79,1 78,9 78,93 0,15 54,73 P2B2 68 69,6 73,1 70,23 2,61 48,36 P2B3
67 72,8 73,9 71,23 3,71 49,04 P2B4 69 74,5 76 73,17 3,68 50,37 P2B5 70 72,9 75,4 72,77 2,70 50,10 akuades 104,4 104,7 104,6 104,57 0,15
72 kontrol 72,9 72,8 73,1 72,93 0,15 50,22 Rata-Rata Tegangan permukaan 54,26 Rata-rata standart deviasi 3,32
ADLN Perpustakaan Universitas Airlangga Lampiran 2. Data nilai emulsifikasi supernatan produksi biosurfaktan Acinetobacter sp. P2(1) (%) pada minyak uji solar
Kode % 1 Jam % 24 Jam
2 19,2
3 1,31 2,76 14,11 10,65
13,8
2 9,32 8,8
1 12,8 15,4
9 14,1
P1B1
4 8,36 4,08 12,9 7,63 4,18 4,43 8,36 8,20
7 4,18 12,5
P1B1
1 1,25 1,94 24,44 21,4
7 22,9
1 11,3
9 22,0
9 23,1
4 25,6
5 24,4
P1B1
5 15,88 7,39 28,18 21,24
4 13,2
3 27,8
6 22,6
6 15,3
3 45,9
P1B2
5 10,6
P1B1
2 26,8
Rata-Rata 4,79 4,39 21,19 17,66
6 5,78 7,12 27,40 26,06
8 32,2
3 18,2
3 27,6
8 31,4
30 20,7
P2B5
2 35,6 7,79 8,92 37,09 35,73
7 44,7
5 45,8 34,7
7 6,25 9,09 8,87 5,1 2,76 2,24 9,42 7,68
P2B4 30,7
8 24,6 8,92 9,09 35,17 31,54
4 28,1
5 41,8
6 28,4
P2B3 45,3 31,7
6 10,51 10,49 19,27 16,76
5 19,8
2 5,06 25,3
7 21,2
P2B2 7,92 28,6
3 23,2
6 3,13 4,97 4,14 10,47 7,82
Sd % 1 Jam
5 5,43 17,4
1 54,5
2 54,8
2 58,4
4 60,5
P1B3 62,5
1 15,4 9,23 11,2 2,26 3,15 16,21 11,94
2 17,8
1 13,6
P1B2 17,2
9 5,92 7,62 19,49 14,16
9 19,5
3 2,06 0,29 60,49 54,52
7 19,4
1 13,5
3 P1B1 25,4
2 AE
1 AE
3 AE
2 AE
1 AE
AE
Jam Rata2 % 24 jam
Sd % 24 jam Rata2 % 1
3 54,2
P1B4 2,94 11,2
3 4,88 9,38 10,9
5 16,3
1 12,1
1 14,4
P1B1
1 8,36 6,1 3,19 2,25 10,82 8,36
3 7,63 10,6
2 10,8
P1B8 14,0
1 1,60 1,57 15,57 14,78
8 13,2
5 14,7
4 14,1
9 7,12 1,25 9,68 5,22 4,17 4,21 7,12 5,38
1 15,2
P1B7 17,3
3 4,7 3,82 6,23 1,71 1,21 9,62 4,92
P1B6 9,8 7,82 11,2
5 1,99 0,170 28,26 16,91
9 16,7
9 17,0
3 16,8
2 26,2
3 30,2
P1B5 28,3
ADLN Perpustakaan Universitas Airlangga Lampiran 3. Grafik karakteristik biosurfaktan (tegangan permukaan, aktivitas emulsifikasi, dan berat kering produk kasar) dalam supernatan kultur Acinetobacter sp. P2(1)
ADLN Perpustakaan Universitas Airlangga
ADLN Perpustakaan Universitas Airlangga
Lampiran 4. Nilai tegangan permukaan produk kasar biosurfaktan Acinetobacter sp. P2(1) dan tween-20 dengan konsentrasi sama dengan CMC (dyne/cm)Standart TP Rata- r TP Perlakuan deviasi (dyne/cm) rata (dyne/cm)
(±) 129,8
Akuades 128 129,77
72 1,75 131,5126,6 Buffer fosfat pH 7 128,2 127,87 70,95 1,13 128,8 96,6
Produk kasar biosurfaktan 97,2 96,87 53,74 0,31 (=CMC) 96,8
101,8 Tween-20 (=CMC) 102,3 102,3 56,76 0,5 102,8 Lampiran 5. Nilai aktivitas emulsifikasi produk kasar biosurfaktan Acinetobacter sp. P2(1) dan tween-20 dengan konsentrasi sama dengan CMC (%) pada minyak uji solar Perlakuan
Persentase kelarutan (%) 1 jam 24 jam Biosurfaktan 42,59 40,34
42,86 40,75 38,1 36,69 Rata-rata 41,18 ± 2,68 39,26 ± 2,24 Tween-20
34,72 32,65 36,67 43,21 35,1 29,99 Rata-rata 35,5 ± 1,031 32,25 ± 2,19
ADLN Perpustakaan Universitas Airlangga
ADLN Perpustakaan Universitas Airlangga
Lampiran 6. Nilai serapan crude enzim lipase Bacillus sp. LII63B dengan UV-Vis dengan nilai λ= 410 pada waktu 16 jamWaktu inkubasi Nilai serapan UV-Vis 1 0,129 16 jam 2 0,127 Rata-rata 0,129 ± 0,001
Kemudian nilai serapan yang didapatkan dimasukkan ke dalam kurva standart p-nitrofenol untuk mengetahui nilai aktivitas enzimnya, Gambar, Kurva standart p-nitrofenol
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Lampiran 7. Nilai blanko kelarutan oil sludge (%) pada masing-masing perlakuan Perlakuan Wo Wt Wb Wb X 8
K 1,037 1,0399 0,0002 0,0016
S 1,1203 1,1251 0,0048 0,0384
B 1,146 1,1509 0,0049 0,0392
E1 1,1001 1,1073 0,0072 0,0576
E2 1,136 1,1394 0,0034 0,0272
E3 1,1842 1,1875 0,0033 0,0264
BE1 1,1698 1,1719 0,0021 0,0168
BE2 1,2067 1,2117 0,005 0,04
BE3 1,1844 1,1893 0,0049 0,0392
- perlakuan blanko tidak disertai dengan penambahan oil sludge
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Lampiran 8. Nilai kelarutan oil sludge (%) pada masing-masing perlakuan
Keterangan : K : penambahan akuades pada oil sludge,
S : penambahan surfaktan sintetis (tween-20) pada oil sludge.
B : penambahan biosurfaktan Acinetobacter sp. P2(1) pada oil sludge. E1 : penambahan 1 mL crude enzim Bacillus sp. LII63B pada oil sludge. E2 : penambahan 2 mL crude enzim Bacillus sp. LII63B pada oil sludge. E3 : penambahan 3 mL crude enzim Bacillus sp. LII63B pada oil sludge. BE1 : penambahan biosurfaktan dan 1 mL crude enzim Bacillus sp. LII63B pada oil sludge.BE2 : penambahan biosurfaktan dan 2 mL crude enzim Bacillus sp. LII63B pada oil sludge. BE3 : penambahan biosurfaktan dan 3 mL crude enzim Bacillus sp. LII63B pada oil sludge. Lampiran 9. Rekapan hasil analisis signifikansi masing-masing perlakuan berdasarkan pada uji Independent Samples T-Test K S B E1 E2 E3 BE1 BE2 BE3 K S S S S S S S S S S S S S S TS TS B S S S TS S S E1 S S S S S E2
S S S TS E3 S S TS BE1
TS S BE2 TS BE3
Keterangan : S : signifikan TS : tidak signifikan
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ADLN Perpustakaan Universitas Airlangga
Lampiran 10. Analisis statistik masing-masing perlakuan pada kelarutan oil sludgeNPar Tests One-Sample Kolmogorov-Smirnov Test Hasil N
27 a,,b
Normal Parameters Mean 30.0554 Std. Deviation 14.32734 Most Extreme Absolute .117 Differences
Positive .090 Negative -.117 Kolmogorov-Smirnov Z .607 Asymp. Sig. (2-tailed) .854 a. Test distribution is Normal.
b. Calculated from data.
Test of Homogeneity of Variances Hasil Levene
Statistic df1 df2 Sig.
3.025
8 18 .024 Robust Tests of Equality of Means
Hasil a Statistic df1 df2 Sig.
Brown- 142.889 8 8.643 .000 Forsythe a. Asymptotically F distributed. H : Tidak ada pengaruh yang signifikan
1 H : Ada pengaruh yang signifikan
α = 0,05 (P value < α) Kesimpulan : Tolak H
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Lampiran 11. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Akuades dengan tween-20; (2) Akuades dengan biosurfaktan Acinetobacter sp. P2(1)T-Test 1. Group Statistics Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K
3 3.5928 .23952 .13829 S 3 33.8520 1.53972 .88896
Independent Samples Test Levene's Test for
Equality of Variances t-test for Equality of Means 95% Confidence Interval of the
Sig.
Difference (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper Hasil Equal 9.190 .039 4 .000 -30.25918 .89965 -32.75701 -27.76136 - variances 33.634 assumed
- Equal 2.097 .001 -30.25918 .89965 -33.96385 -26.55452 variances 33.634 not assumed
T-Test 2. Group Statistics Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K
3 3.5928 .23952 .13829 B 3 22.0359 2.87425 1.65945
Independent Samples Test
Levene's Test for Equality of
Variances t-test for Equality of Means 95% Confidence
Interval of the Difference
Sig. (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper
- Hasil Equal variances 3.338 .142 4 .000 -18.44311 1.66520 - - assumed 11.076 23.06646 13.81977
- Equal variances 2.028 .008 -18.44311 1.66520 - - not assumed 11.076 25.51467 11.37156
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Lampiran 12. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Akuades dengan crude enzim lipase 12,5% (v/v);(2) Akuades dengan crude enzim lipase 25% (v/v)
1. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K 3 3.5928 .23952 .13829 E1 3 12.6946 2.15565 1.24457 Independent Samples Test
Levene's Test for Equality of Variances t-test for Equality of Means 95% Confidence
Interval of the Sig.
Difference (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper
- Hasil Equal 3.122 .152 4 .002 -9.10183 1.25223 variances 7.269 12.57857 5.62509 assumed
- Equal - 2.049 .017 -9.10183 1.25223 - variances not 7.269 14.36722 3.83644 assumed
2. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K 3 3.5928 .23952 .13829 E2 3 40.9581 .23952 .13829 Independent Samples Test
Levene's Test for Equality of Variances t-test for Equality of Means 95% Confidence
Interval of the Sig.
Difference (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper
- Hasil Equal .000 1.000 4 .000 -37.36527 .19557 variances 191.060 37.90825 36.82229 assumed
- Equal 4.000 .000 -37.36527 .19557 variances 191.060 37.90825 36.82229 not assumed
- 39.69654 Equal variances not assumed
- 39.69654
- 17.64303
- 12.669
- 15.00826
- 205.757 4.000 .000 -40.23952 .19557 - 40.78250
2.024 .006 -22.59481 1.78350 - 30.18136
Equal variances not assumed
27.54659
11.188 .029 - 12.669 4 .000 -22.59481 1.78350 -
Lower Upper Hasil Equal variances assumed
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Independent Samples Test
205.757 4 .000 -40.23952 .19557 - 40.78250
Difference Lower Upper Hasil Equal variances assumed .000 1.000 -
Std. Error Difference 95% Confidence Interval of the
(2- tailed) Mean Difference
Sig.
Levene's Test for Equality of Variances t-test for Equality of Means F Sig. t df
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K 3 3.5928 .23952 .13829 BE1 3 26.1876 3.07981 1.77813 Independent Samples Test
2. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K 3 3.5928 .23952 .13829 E3 3 43.8323 .23952 .13829
1. T-Test
Group Statistics
Lampiran 13. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Akuades dengan crude enzim lipase 37,5% (v/v); (2) Akuades dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 12,5% (v/v)
ADLN Perpustakaan Universitas Airlangga
ADLN Perpustakaan Universitas Airlangga
33.31093
2.002 .018 -43.03393 5.91627 - 68.46297
Equal variances not assumed
59.46013
15.037 .018 - 7.274 4 .002 -43.03393 5.91627 -
Lower Upper Hasil Equal variances assumed
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Independent Samples Test
2.023 .004 -28.18363 1.84671 - 36.04508
Equal variances not assumed
7.269 .054 - 15.262 4 .000 -28.18363 1.84671 -
Independent Samples Test
BE2 3 31.7764 3.18962 1.84153
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Lower Upper Hasil Equal variances assumed
Lampiran 14. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Akuades dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 25% (v/v); (2) Akuades dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v)
1. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K 3 3.5928 .23952 .13829
- 23.05634
- 15.262
- 20.32219
2. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil K 3 3.5928 .23952 .13829
BE3 3 46.6267 10.24448 5.91465
- 26.60773
- 7.274
- 17.60490
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Lampiran 15. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Tween-20 dengan biosurfaktan Acintobacter sp.P2(1); (2) Tween-20 dengan crude enzim lipase 12,5% (v/v)1. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil S 3 33.8520 1.53972 .88896
B 3 22.0359 2.87425 1.65945
Independent Samples Test
Levene's Test for Equality of
Variances t-test for Equality of Means 95% Confidence
Interval of the Difference
Sig. (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper
Hasil Equal variances .543 .502 6.277 4 .003 11.81607 1.88256 6.58925 17.04288 assumed Equal variances 6.277 3.061 .008 11.81607 1.88256 5.89142 17.74072 not assumed
2. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil S 3 33.8520 1.53972 .88896 E1 3 12.6946 2.15565 1.24457
Independent Samples Test Levene's Test for Equality of
Variances t-test for Equality of Means 95% Confidence Interval of the Difference
Sig. (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper Hasil Equal variances .114 .752 13.833 4 .000 21.15735 1.52944 16.91094 25.40376 assumed
Equal variances 13.833 3.619 .000 21.15735 1.52944 16.72883 25.58587 not assumed
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Lampiran 16. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Tween-20 dengan crude enzim lipase 25% (v/v);(2) Tween-20 dengan crude enzim lipase 37,5% (v/v)
1. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil S 3 33.8520 1.53972 .88896 E2 3 40.9581 .23952 .13829
Independent Samples Test Levene's Test for Equality of Variances t-test for Equality of Means
95% Confidence Interval of the Difference Sig. (2- Mean Std. Error
F Sig. t df tailed) Difference Difference Lower Upper Hasil Equal variances 9.190 .039 -7.899 4 .001 -7.10609 .89965 -9.60391 -4.60826 assumed
Equal variances -7.899 2.097 .014 -7.10609 .89965 -10.81075 -3.40142 not assumed
2. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil S 3 33.8520 1.53972 .88896
E3 3 43.8323 .23952 .13829
Independent Samples Test
Levene's Test for Equality of
Variances t-test for Equality of Means 95% Confidence
Interval of the Difference
Sig. (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper
- Hasil Equal variances 9.190 .039 4 .000 -9.98034 .89965 - -7.48252 assumed 11.094 12.47816
2.097 - Equal variances .007 -9.98034 .89965 -6.27568 - not assumed 11.094 13.68500
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Lampiran 17. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Tween-20 dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 12,5% (v/v); (2) Tween-20 dengan biosurfaktan 37,5% (v/v) crude enzim lipase 25% (v/v)1. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil S 3 33.8520 1.53972 .88896 BE1 3 26.1876 3.07981 1.77813
Independent Samples Test Levene's Test for Equality of Variances t-test for Equality of Means
95% Confidence Interval of the Difference Sig. (2- Mean Std. Error
F Sig. t df tailed) Difference Difference Lower Upper Hasil Equal variances 2.617 .181 3.855 4 .018 7.66437 1.98796 2.14491 13.18383 assumed
Equal variances 3.855 2.941 .032 7.66437 1.98796 1.26535 14.06339 not assumed
2. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil S 3 33.8520 1.53972 .88896
BE2 3 31.7764 3.18962 1.84153
Independent Samples Test
Levene's Test for Equality of
Variances t-test for Equality of Means 95% Confidence
Interval of the Difference
Sig. (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper
Hasil Equal variances 1.820 .249 1.015 4 .368 2.07555 2.04486 -3.60190 7.75300 assumed Equal variances 1.015 2.884 .388 2.07555 2.04486 -4.58260 8.73369 not assumed
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29.38090 3.83140
4.503 3.709 .013 9.34128 2.07430 3.39957 15.28300
.160 .710 4.503 4 .011 9.34128 2.07430 3.58210 15.10046 Equal variances not assumed
Lower Upper Hasil Equal variances assumed
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Independent Samples Test
11.92312
2.090 .161 -12.77475 5.98108 - 37.47263
Equal variances not assumed
11.079 .029 - 2.136 4 .100 -12.77475 5.98108 -
Lampiran 18. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Tween-20 dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v); (2) Biosurfaktan Acinetobacter sp.P2(1) dengan crude enzim lipase 12,5% (v/v)
Lower Upper Hasil Equal variances assumed
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Independent Samples Test
E1 3 12.6946 2.15565 1.24457
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil B 3 22.0359 2.87425 1.65945
2. T-Test
Group Statistics
BE3 3 46.6267 10.24448 5.91465
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil S 3 33.8520 1.53972 .88896
1. T-Test
Group Statistics
- 2.136
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23.54550
2.028 .005 -21.79641 1.66520 - 28.86796
Equal variances not assumed
26.41975
3.338 .142 - 13.089 4 .000 -21.79641 1.66520 -
Lower Upper Hasil Equal variances assumed
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Independent Samples Test
2.028 .007 -18.92216 1.66520 - 25.99371
Equal variances not assumed
3.338 .142 - 11.363 4 .000 -18.92216 1.66520 -
Independent Samples Test
E2 3 40.9581 .23952 .13829
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Lower Upper Hasil Equal variances assumed
Lampiran 19. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Biosurfaktan Acinetobacter sp.P2(1) dengan crude enzim lipase 25% (v/v); (2) Biosurfaktan Acinetobacter sp.P2(1) dengan crude enzim lipase 37,5% (v/v)
1. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil B 3 22.0359 2.87425 1.65945
- 14.29881
- 11.363
- 11.85060
2. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil B 3 22.0359 2.87425 1.65945
E3 3 43.8323 .23952 .13829
- 17.17306
- 13.089
- 14.72485
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10.90452 2.60113
3.957 .017 -9.74052 2.47891 -16.65238 -2.82865
Equal variances not assumed
.116 .751 - 3.929 4 .017 -9.74052 2.47891 -16.62308 -2.85796
Lower Upper Hasil Equal variances assumed
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Independent Samples Test
2.61381
3.981 .163 -4.15170 2.43218 - 10.91720
Equal variances not assumed
.136 .731 - 1.707 4 .163 -4.15170 2.43218 -
Independent Samples Test
BE1 3 26.1876 3.07981 1.77813
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Lower Upper Hasil Equal variances assumed
Lampiran 20. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Biosurfaktan Acinetobacter sp.P2(1) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 12,5% (v/v); (2) Biosurfaktan Acinetobacter sp.P2(1) dengan biosurfaktan 37,5% (v/v) crude enzim lipase 25% (v/v)
1. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil B 3 22.0359 2.87425 1.65945
- 1.707
2. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil B 3 22.0359 2.87425 1.65945
BE2 3 31.7764 3.18962 1.84153
- 3.929
ADLN Perpustakaan Universitas Airlangga
7.293 .054 - 4.003 4 .016 -24.59082 6.14304 -
2.049 .002 -28.26344 1.25223 - 33.52883
Equal variances not assumed
31.74018
3.122 .152 - 22.571 4 .000 -28.26344 1.25223 -
Lower Upper Hasil Equal variances assumed
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Independent Samples Test
2.313 .045 -24.59082 6.14304 - 47.87440
41.64662
Lower Upper Hasil Equal variances assumed
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Independent Samples Test
E2 3 40.9581 .23952 .13829
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E1 3 12.6946 2.15565 1.24457
2. T-Test
Group Statistics
BE3 3 46.6267 10.24448 5.91465
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil B 3 22.0359 2.87425 1.65945
1. T-Test
Group Statistics
Lampiran 21. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Biosurfaktan Acinetobacter sp.P2(1) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v); (2) Crude enzim lipase 12,5% (v/v) dengan crude enzim lipase 25% (v/v)
- 7.53501 Equal variances not assumed
- 4.003
- 1.30723
- 24.78670
- 22.571
- 22.99805
ADLN Perpustakaan Universitas Airlangga
Lampiran 22. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Crude enzim lipase 12,5% (v/v) dengan crude enzim lipase 37,5% (v/v); (2) Crude enzim lipase 12,5% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 12,5% (v/v)1. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E1 3 12.6946 2.15565 1.24457
E3 3 43.8323 .23952 .13829
Independent Samples Test
Levene's Test for Equality of
Variances t-test for Equality of Means 95% Confidence
Interval of the Difference
Sig. (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper
4 - Hasil Equal variances - 3.122 .152 .000 -31.13769 1.25223 - assumed 24.866 34.61443 27.66095 Equal variances - 2.049 .001 -31.13769 1.25223 - - not assumed 24.866 36.40308 25.87230
2. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E1 3 12.6946 2.15565 1.24457
BE1 3 26.1876 3.07981 1.77813
Independent Samples Test
Levene's Test for Equality of
Variances t-test for Equality of Means 95% Confidence
Interval of the Difference
Sig. (2- Mean Std. Error F Sig. t df tailed) Difference Difference Lower Upper
- Hasil Equal variances .875 .402 4 .003 -13.49298 2.17041 -19.51901 -7.46695 assumed 6.217
- Equal variances 3.580 .005 -13.49298 2.17041 -19.80807 -7.17789 not assumed 6.217
ADLN Perpustakaan Universitas Airlangga
25.25286
2.177 .025 -33.93210 6.04418 - 58.01562
Equal variances not assumed
50.71343
9.277 .038 - 5.614 4 .005 -33.93210 6.04418 -
Lower Upper Hasil Equal variances assumed
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Independent Samples Test
3.512 .002 -19.08180 2.22265 - 25.60651
Equal variances not assumed
.703 .449 - 8.585 4 .001 -19.08180 2.22265 -
Independent Samples Test
BE2 3 31.7764 3.18962 1.84153
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Lower Upper Hasil Equal variances assumed
Lampiran 23. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Crude enzim lipase 12,5% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 25% (v/v); (2) Crude enzim lipase 12,5% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v)
1. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E1 3 12.6946 2.15565 1.24457
- 12.91074
- 8.585
- 12.55709
2. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E1 3 12.6946 2.15565 1.24457
BE3 3 46.6267 10.24448 5.91465
- 17.15078
- 5.614
- 9.84859
ADLN Perpustakaan Universitas Airlangga
.000 1.000 - 14.697 4 .000 -2.87425 .19557 -3.41724 -2.33127
8.282 2.024 .014 14.77046 1.78350 7.18391 22.35701
11.188 .029 8.282 4 .001 14.77046 1.78350 9.81868 19.72224 Equal variances not assumed
Lower Upper Hasil Equal variances assumed
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Independent Samples Test
4.000 .000 -2.87425 .19557 -3.41724 -2.33127
Equal variances not assumed
Lower Upper Hasil Equal variances assumed
Lampiran 24. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Crude enzim lipase 25% (v/v) dengan crude enzim lipase 37,5% (v/v); (2) Crude enzim lipase 25% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 12,5% (v/v)
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Independent Samples Test
BE1 3 26.1876 3.07981 1.77813
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E2 3 40.9581 .23952 .13829
2. T-Test
Group Statistics
E3 3 43.8323 .23952 .13829
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E2 3 40.9581 .23952 .13829
1. T-Test
Group Statistics
- 14.697
ADLN Perpustakaan Universitas Airlangga
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E2 3 40.9581 .23952 .13829
31.09770 19.76037
10.75754 Equal variances not assumed
15.037 .018 -.958 4 .392 -5.66866 5.91627 - 22.09486
Lower Upper Hasil Equal variances assumed
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Independent Samples Test
BE3 3 46.6267 10.24448 5.91465
2. T-Test
Group Statistics
Lampiran 25. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Crude enzim lipase 25% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 25% (v/v); (2) Crude enzim lipase 25% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v)
4.972 2.023 .037 9.18164 1.84671 1.32019 17.04308
7.269 .054 4.972 4 .008 9.18164 1.84671 4.05434 14.30893 Equal variances not assumed
Lower Upper Hasil Equal variances assumed
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Independent Samples Test
BE2 3 31.7764 3.18962 1.84153
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E2 3 40.9581 .23952 .13829
1. T-Test
Group Statistics
- .958 2.002 .439 -5.66866 5.91627 -
1. T-Test
Group Statistics
BE2 3 31.7764 3.18962 1.84153
6.528 2.023 .022 12.05589 1.84671 4.19444 19.91733
7.269 .054 6.528 4 .003 12.05589 1.84671 6.92859 17.18318 Equal variances not assumed
Lower Upper Hasil Equal variances assumed
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Independent Samples Test
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E3 3 43.8323 .23952 .13829
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E3 3 41.8363 3.46547 2.00079
2. T-Test
Group Statistics
Equal variances not assumed 5.846 3.946 .004 15.64870 2.67674 8.17630 23.12111
Hasil Equal variances assumed .116 .751 5.846 4 .004 15.64870 2.67674 8.21689 23.08051
Std. Error Difference Lower Upper
Mean Difference
F Sig. t df Sig. (2- tailed)
Interval of the Difference
Variances t-test for Equality of Means 95% Confidence
Levene's Test for Equality of
Independent Samples Test
BE1 3 26.1876 3.07981 1.77813
ADLN Perpustakaan Universitas Airlangga
ADLN Perpustakaan Universitas Airlangga
13.63179 Equal variances not assumed
1.52197
3.995 .094 -5.58882 2.55988 - 12.69961
Equal variances not assumed
12.69618 1.51853
.000 1.000 - 2.183 4 .094 -5.58882 2.55988 -
Lower Upper Hasil Equal variances assumed
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Independent Samples Test
28.22345 22.63462
15.037 .018 -.472 4 .661 -2.79441 5.91627 - 19.22061
Independent Samples Test
BE3 3 46.6267 10.24448 5.91465
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Lower Upper Hasil Equal variances assumed
Lampiran 27. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Crude enzim lipase 37,5% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v); (2) Biosurfaktan 37,5% (v/v) dan crude enzim lipase 12,5% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 25% (v/v)
1. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil E3 3 43.8323 .23952 .13829
- .472 2.002 .683 -2.79441 5.91627 -
2. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil BE1 3 26.1876 3.07981 1.77813
BE2 3 31.7764 3.18962 1.84153
- 2.183
ADLN Perpustakaan Universitas Airlangga
Difference 95% Confidence
2.61497
Independent Samples Test
Levene's Test for Equality of
Variances t-test for Equality of Means F Sig. t df
Sig. (2- tailed) Mean
Difference Std. Error
Interval of the Difference
37.58687
Lower Upper Hasil Equal variances assumed
6.656 .061 - 2.397 4 .075 -14.85030 6.19470 -
32.04955 2.34895
Equal variances not assumed
2.384 .118 -14.85030 6.19470 - 37.78341
8.08281
2.359 .064 -20.43912 6.17615 - 43.49321
7.005 .057 - 3.309 4 .030 -20.43912 6.17615 -
Independent Samples Test
BE3 3 46.6267 10.24448 5.91465
Interval of the Difference
Difference 95% Confidence
Difference Std. Error
Sig. (2- tailed) Mean
Variances t-test for Equality of Means F Sig. t df
Levene's Test for Equality of
Lower Upper Hasil Equal variances assumed
Lampiran 28. Analisis statistik perbandingan kelarutan oil sludge antara perlakuan: (1) Biosurfaktan 37,5% (v/v) dan crude enzim lipase 12,5% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v); (2) Biosurfaktan 37,5% (v/v) dan crude enzim lipase 25% (v/v) dengan biosurfaktan 37,5% (v/v) dan crude enzim lipase 37,5% (v/v)
1. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil BE1 3 26.1876 3.07981 1.77813
- 3.29137 Equal variances not assumed
- 3.309
2. T-Test
Group Statistics
Perlakuan N Mean Std. Deviation Std. Error Mean Hasil BE2 3 31.7764 3.18962 1.84153
BE3 3 46.6267 10.24448 5.91465
- 2.397
ADLN Perpustakaan Universitas Airlangga
Lampiran 29. Produksi Biosurfaktan Acinetobacter sp.P2(1)1
2
3
4
5 Keterangan : 1 : kultur yang diproduksi setelah 3 hari 2 : kontrol media produksi 3 : supernatan kultur setelah disentrifugasi pada saat panen 4 : hasil liofilisasi supernatan 5 : produk biosurfaktan keseluruhan
ADLN Perpustakaan Universitas Airlangga
Lampiran 30. Produksi Crude enzim lipase Bacillus sp. LII63B1
2
3
4
5
6 Keterangan : 1 : media Bushnell Hash + 1% minyak untuk uji aktivitas enzim 2 : media NB 3 : isolat Bacillus sp. LII63B dalam media NB 4 : media produksi crude enzim lipase Bacillus sp. LII63B sebelum
5: media produksi crude enzim lipase Bacillus sp. LII63B sesudah inkubasi 6 : isolat Bacillus sp. LII63B dengan perbesaran 1000 X menggunakan mikroskop. Lampiran 31. Uji kelarutan oil sludge pada masing-masing perlakuan B
2
1
3 B
3 B
ADLN Perpustakaan Universitas Airlangga
1
2
1
2
3 S B
1
2
1
2
3 K B
1
2
3 B
3 B
B
3 B
3 E2 B
3 E3
ADLN Perpustakaan Universitas Airlangga
1
2
1
2
1
2
2
1
2
3 E1 B
1
2