Pengaruh Konsentrasi Air Perasan Jeruk Nipis (Citrus Aurantifolia) dalam Menghambat Pertumbuhan Bakteri Streptococcus Pyogenes Secara in vitro
ii
ABSTRAK
Pendahuluan Jeruk nipis (Citrus aurantifolia ) merupakan tanaman yang
memiliki berbagai macam manfaat. Salah satunya sebagai antibakteri. Perbedaan
konsentrasi air perasan jeruk nipis diduga berpengaruh dalam menghambat
pertumbuhan bakteri Streptococcu pyogenes secara in vitro.
Metode Tujuan penelitian ini adalah untuk melihat pengaruh konsentrasi air
perasan jeruk nipis (Citrus aurantifolia) dalam menghambat pertumbuhan bakteri
Streptococcus pyogenes secara in vitro. Penelitian ini dilakukan dengan metode
eksperimental dengan desain Posttest Only Control Group Design . Sampel
penelitian berasal dari biakan specimen klinis yang telah diidentifikasi sebagai
bakteri Streptococcus pyogenes di Laboratorium Mikrobiologi FK USU
dengan empat kali pengulangan yang diambil dengan menggunakan rumus
Federer. Data hasil penelitian diolah dengan uji Kruskal-Wallis dan dilanjutkan
dengan uji Regresi Linier.
Hasil Berdasarkan uji Kruskal-Wallis didapati nilai p sebesar 0,000 (CI 95%). Hal
ini menunjukkan bahwa varians zona hambat yang terbentuk pada lima
konsentrasi yang berbeda memiliki perbedaan yang bermakna. Sedangkan pada
uji Regresi Liner didapatkan nilai p sebesar ,000 (CI 95%) yang membuktikan
bahwa terdapat pengaruh konsentrasi dalam menghambat pertumbuhan bakteri
Streptococcus pyogenes secara in vitro.
Diskusi Dari hasil analisis data tersebut dapat disimpulkan bahwa semakin tinggi
konsentrasi air perasan jeruk nipis, maka daya hambat terhadap bakteri
Streptococcus pyogenes secara in vitro semakin besar. Untuk ke depannya
diharapkan akan ada penelitian-penelitian lain mengenai pengaruh konsentrasi air
jeruk nipis terhadap bakteri lainnya.
Kata Kunci: Air Perasan Jeruk Nipis, Streptococcus pyogenes, Konsentrasi, Daya
Hambat
Universitas Sumatera Utara
iii
ABSTRACT
Introduction Lime (Citrus aurantifolia) is a plant which has many benefits. One
of them is as an antibacteria. The differences of concentration of lime fruit
squeeze was assumed to take impact in inhibit the growth of the bacteria
Streptococcus pyogenes in vitro condition.
Methods The purpose of this research was to determine the effect of concentration
of lime fruit (Citrus aurantifolia) squeeze towards the growth of the bacteria
Streptococcus pyogenes in vitro condition. This research was conducted with
experimental methods with Post Only Control Group Design.
relationship between the history of asthma and atopic dermatitis for the event of
allergic rhinitis by the 13-14 years old school students in Pasaman regency.. The
population in this research were all 13-14 years old school students in Pasaman
regency. Samples were 190 students, taken by cluster sampling method. Research
data was analyzed with Chi Square hypothesis test.
Results Based on the Chi Square test, the p value is 0,000 (CI 95%) for both
history of asthma and atopic dermatitis. It shows that there is a relationship
between the history of asthma and atopic dermatitis for the event of allergic
rhinitis. The prevalence ratio for history of asthma and atopic dermatitis are
2,079 and 2,381, respectively, so it can be assumed that the history of asthma and
atopic dermatitis are the risk factors for allergic rhinitis.
Discussion The conclusion of this research is there is a relationship between the
history of asthma and atopic dermatitis and the event of allergic rhinitis by the
13-14 years old school students in Pasaman regency. The limitations of this
research are: a limited amount of time in doing this research, some weaknesses of
the design of the research and sampling method, the data collection was only
based on the questionnaire, and also the competency of school students in
answering the questions from the questionnaire. For the future, hoped that there
will another researches about the history of asthma, atopic dermatitis and other
factors as the risk factors for allergic rhinitis.
Key words: Allergic Rhinitis, Asthma, Atopic Dermatitis, Risk Factor, School
Students, 13-14 years old
Universitas Sumatera Utara
ABSTRAK
Pendahuluan Jeruk nipis (Citrus aurantifolia ) merupakan tanaman yang
memiliki berbagai macam manfaat. Salah satunya sebagai antibakteri. Perbedaan
konsentrasi air perasan jeruk nipis diduga berpengaruh dalam menghambat
pertumbuhan bakteri Streptococcu pyogenes secara in vitro.
Metode Tujuan penelitian ini adalah untuk melihat pengaruh konsentrasi air
perasan jeruk nipis (Citrus aurantifolia) dalam menghambat pertumbuhan bakteri
Streptococcus pyogenes secara in vitro. Penelitian ini dilakukan dengan metode
eksperimental dengan desain Posttest Only Control Group Design . Sampel
penelitian berasal dari biakan specimen klinis yang telah diidentifikasi sebagai
bakteri Streptococcus pyogenes di Laboratorium Mikrobiologi FK USU
dengan empat kali pengulangan yang diambil dengan menggunakan rumus
Federer. Data hasil penelitian diolah dengan uji Kruskal-Wallis dan dilanjutkan
dengan uji Regresi Linier.
Hasil Berdasarkan uji Kruskal-Wallis didapati nilai p sebesar 0,000 (CI 95%). Hal
ini menunjukkan bahwa varians zona hambat yang terbentuk pada lima
konsentrasi yang berbeda memiliki perbedaan yang bermakna. Sedangkan pada
uji Regresi Liner didapatkan nilai p sebesar ,000 (CI 95%) yang membuktikan
bahwa terdapat pengaruh konsentrasi dalam menghambat pertumbuhan bakteri
Streptococcus pyogenes secara in vitro.
Diskusi Dari hasil analisis data tersebut dapat disimpulkan bahwa semakin tinggi
konsentrasi air perasan jeruk nipis, maka daya hambat terhadap bakteri
Streptococcus pyogenes secara in vitro semakin besar. Untuk ke depannya
diharapkan akan ada penelitian-penelitian lain mengenai pengaruh konsentrasi air
jeruk nipis terhadap bakteri lainnya.
Kata Kunci: Air Perasan Jeruk Nipis, Streptococcus pyogenes, Konsentrasi, Daya
Hambat
Universitas Sumatera Utara
iii
ABSTRACT
Introduction Lime (Citrus aurantifolia) is a plant which has many benefits. One
of them is as an antibacteria. The differences of concentration of lime fruit
squeeze was assumed to take impact in inhibit the growth of the bacteria
Streptococcus pyogenes in vitro condition.
Methods The purpose of this research was to determine the effect of concentration
of lime fruit (Citrus aurantifolia) squeeze towards the growth of the bacteria
Streptococcus pyogenes in vitro condition. This research was conducted with
experimental methods with Post Only Control Group Design.
relationship between the history of asthma and atopic dermatitis for the event of
allergic rhinitis by the 13-14 years old school students in Pasaman regency.. The
population in this research were all 13-14 years old school students in Pasaman
regency. Samples were 190 students, taken by cluster sampling method. Research
data was analyzed with Chi Square hypothesis test.
Results Based on the Chi Square test, the p value is 0,000 (CI 95%) for both
history of asthma and atopic dermatitis. It shows that there is a relationship
between the history of asthma and atopic dermatitis for the event of allergic
rhinitis. The prevalence ratio for history of asthma and atopic dermatitis are
2,079 and 2,381, respectively, so it can be assumed that the history of asthma and
atopic dermatitis are the risk factors for allergic rhinitis.
Discussion The conclusion of this research is there is a relationship between the
history of asthma and atopic dermatitis and the event of allergic rhinitis by the
13-14 years old school students in Pasaman regency. The limitations of this
research are: a limited amount of time in doing this research, some weaknesses of
the design of the research and sampling method, the data collection was only
based on the questionnaire, and also the competency of school students in
answering the questions from the questionnaire. For the future, hoped that there
will another researches about the history of asthma, atopic dermatitis and other
factors as the risk factors for allergic rhinitis.
Key words: Allergic Rhinitis, Asthma, Atopic Dermatitis, Risk Factor, School
Students, 13-14 years old
Universitas Sumatera Utara