Institutional Repository | Satya Wacana Christian University: Aktivitas Antioksidan dan Kadar Tempe Satu Kali Perebusan dari Kedelai (Glycine max L Merr) Lokal var. Grobogan dan Impor

29 LAMPIRAN

Lampiran 1. Gambar Tempe Satu kali Perebusan dengan Berbagai Perbandingan Kedelai Lokal dan Impor

Tempe kedelai lokal 100% Tempe kedelai lokal:impor = 75% : 25%

Tempe kedelai lokal:impor = 50% : 50% Tempe kedelai lokal:impor = 25%: 75%

Lampiran 2. Hasil Pengukuran Kadar Air Tempe

Ulangan

Persentase (Lokal : Impor)

Kadar Air (%)

I II III IV V Rata-rata

100% : 0% 64,48 63,75 63,43 64,54 64,48 64,14 75% : 25% 63,72 63,16 63,34 64,23 64,41 63,77 50% : 50% 63,69 63,05 63,00 64,01 63,73 63,44 25% : 75% 63,39 62,81 62,76 63,98 63,42 63,27 0% : 100% 62,69 62,59 62,62 62,66 62,68 62,65

31

Lampiran 3. Hasil Pengukuran Absorbansi Sampel Tabel 3.1. Absorbansi Aktivitas Antioksidan Tempe

Ulangan

Konsentrasi (Lokal : Impor)

Absorbansi ( λ = 700 nm)

I II III IV V

100% : 0% 0,843 0,873 0,806 0,850 0,8369 75% : 25% 0,831 0,847 0,794 0,847 0,819 50% : 50% 0,828 0,828 0,786 0,826 0,813 25% : 75% 0,818 0,797 0,731 0,795 0,773 0% : 100% 0,770 0,786 0,726 0,789 0,763

Tabel 3.2. Absorbansi Kadar Fenolik Total Tempe Ulangan

Konsentrasi (Lokal : Impor)

Absorbansi ( λ = 765 nm)

I II III IV V

100% : 0% 0,873 0,882 0,823 0,878 0,860 75% : 25% 0,868 0,869 0,813 0,861 0,847 50% : 50% 0,843 0,842 0,808 0,843 0,825 25% : 75% 0,822 0,811 0,799 0,833 0,803 0% : 100% 0,803 0,773 0,751 0,825 0,736

Lampiran 4. Kurva Standar K4Fe(CN)6

Contoh Perhitungan Aktivitas Antioksidan Tempe Sampel : Tempe Kedelai Lokal 100 % (Ulangan I) Persamaaan Kurva Standar: y = 0,369 x + 0,047 Absorbansi (700 nm) = 0,843

0,843 = 0,369 x + 0,047 0,369 x = 0,843 – 0,047 x =

x = 2,1572 mM Konsentrasi (mM) Absorbansi (700nm) 0,271 0,543 0,814 1,086 1,357 1,629 1,900 2,172 2,443 2,715 0,124 0,251 0,357 0,462 0,566 0,674 0,706 0,856 0,950 1,049

33 Lampiran 4 (Lanjutan)

Dalam 100 ml = 0,1 L x 2,1572 mM = 0,21572 mek / 5,01 gram

= 4,306 x 10-2mek / gram berat basah Kadar Air Sampel = 64,48 %

Berat basah sampel = 5,01 gram, maka Berat air =

Berat kering sampel = (5,01 – 3,2304) gram = 1,7796 gram Berdasarkan berat kering =

Lampiran 5. Kurva Standar Asam Galat

Contoh Perhitungan Kadar Fenolik Total Tempe Sampel : Tempe Kedelai Lokal 100 % (Ulangan I) Persamaaan Kurva Standar: y = 9,724 x – 0,009 Absorbansi (765 nm) = 0,873

0,873 = 9,724 x -0,009 9,724 x = 0,873 – 0,037 x =

x = 9,0703 x 10-2 mg/ml Konsentrasi

(mg/ml)

Absorbansi ( 765 nm)

0,01 0,081

0,02 0,188

0,03 0,265

0,04 0,396

0,05 0,496

0,06 0,568

0,07 0,656

0,08 0,772

0,09 0,874

35 Lampiran 5 (Lanjutan)

Dalam 100 ml = 100 x 9,0703 x 10-2 mg/ml = 9,0703 mg

Kadar Air Sampel = 64,48 % Berat basah sampel = 5,01 gram, maka Berat air =

Berat kering sampel = (5,01 – 3,2304) gram = 1,7796 gram Berdasarkan berat kering =

Lampiran 6. Hasil Perhitungan Aktivitas Antioksidan Tempe Ulangan

Persentase (Lokal : Impor)

Aktivitas Antioksidan (mek / gram)

I II III IV V Rata-rata

100% : 0% 12,1218x10

-2

12,3259x10-2 11,2491x10-2 12,2012x10-2 11,9651x10-2 11,9726x10-2 75% : 25% 11,7128x10

-2

11,7462x10-2 11,0442x10-2 12,0975x10-2 11,6864x10-2 11,6574x10-2 50% : 50% 11,6113x10

-2

11,4331x10-2 10,8254x10-2 11,6616x10-2 11,4242x10-2 11,3911x10-2 25% : 75% 11,4144x10

-2

10,9304x10-2 9,9357x10-2 11,2106x10-2 10,6935x10-2 10,8369x10-2 0% : 100% 10,5028x10

-2

37

Lampiran 7. Hasil Perhitungan Kadar Fenolik Total Tempe Ulangan

Konsentrasi (Lokal : Impor)

Kadar Fenolik Total ( mg / gram)

I _{II III IV V Rata-rata}

100% : 0% 5,0968 5,0454 4,6793 5,1142 4,9819 4,9835 75% : 25% 4,9718 4,8920 4,6117 4,9924 4,9173 4,87704 50% : 50% 4,8068 4,7275 4,5416 4,8399 4,7119 4,72714 25% : 75% 4,6686 4,5349 4,4537 4,7887 4,5385 4,59688 0% : 100% 4,4763 4,2994 4,1650 4,5848 4,0978 4,32466

Lampiran 8. Analisa Data Aktivitas Antioksidan Tempe

Ulangan Persentase Kedelai (Lokal : Impor) Tu Xu du W

100%:0% 75%:25% 50%:50% 25%:75 0%:100%

1 0,1212 0,1171 0,1161 0,1141 0,105 0,5736 0,1147 0,0021 0,000141

2 0,1233 0,1175 0,1143 0,1093 0,1071 0,5714 0,1143 0,0014 0,000158 3 0,1125 0,1104 0,1083 0,0994 0,0981 0,5286 0,1057 -0,0069 0,000161

4 0,122 0,1209 0,1166 0,1121 0,1075 0,5792 0,1158 0,0033 0,000151

5 0,1197 0,1169 0,1162 0,1069 0,1038 0,5615 0,1123 0,0001 0,000167

Tp 0,5986 0,5829 0,5696 0,5418 0,5214 2,8143

Xp 0,1197 0,1166 0,1139 0,1084 0,1043 (X…) 0,1126

dp 0,007153 0,004001 0,0013388 -0,004204 -0,008288 SD 0,00425 0,00381 0,0033333 0,005733 0,003794 SE 0,00528 0,00473 0,00414 0,00712 0,00471

39 Lampiran 8 (Lanjutan)

W1= {(0,1212 x 0,007153) + (0,1171x 0,004001) + (0,1161x0,0013388) + (0,1141x-0,004204) + (0,105x-0,008288)} = 0,000141

W2= {(0,1233x 0,007153) + (0,1175x 0,004001) + (0,1143x0,0013388) + (0,1093x-0,004204) + (0,1071x-0,008288)} = 0,000158

W3= {(0,1125x 0,007153) + (0,1104x 0,004001) + (0,1083x0,0013388) + (0,0994x-0,004204) + (0,0981x-0,008288)} = 0,000161

W4= {(0,122x 0,007153) + (0,1209x 0,004001) + (0,1166x0,0013388) + (0,1121x-0,004204) + (0,1075x-0,008288)} = 0,000151

W5= {(0,1197x 0,007153) + (0,1169x 0,004001) + (0,1162x0,0013388) + (0,1069x-0,004204) + (0,1038x-0,008288)} = 0,000167

N = Wix du

={(0,000141x0,0021) + (0,000158x 0,0014) + (0,000161x -0,0069) + (0,000151x 0,0033) + (0,000167x0,0001) = -8,3 x 10-8

D = du2x dp2

= {[(0,0021)2+ (0,0014)2+ (-0,0069)2+ (0,0033)2 + (0,0001)2]x [(0,007153)2+ (0,004001)2+ (0,0013388)2+ (-0,004204)2+ (-0,008288)2]} = 1,0138 x 10-8

FK = = = 31,6818 x 10-2

JK Non Aditivitas = = = 7,0147x 10-7 JK Total = p2– FK

= {(0,1212)2 + (0,1171)2+ …. +(0,1038)2} -31,6818 x 10-2 = 31,7959 x 10-2 - 31,6818 x 10-2

= 11,4056 x 10-4

JK Ulangan = - FK

Lampiran 8 (Lanjutan)

JK Perlakuan = - FK

= – 31,6818 x 10-2 = 7,7664 x 10-4 JK Galad Acak = JK Total – JK Ulangan – JK Perlakuan

= (11,4056 x 10-4) - (3,2634 x 10-4)– (7,7664 x 10-4) = 3,7581 x 10-4

DASIRA UJI ADDITIVITAS

Sumber Ragam Db JK KT F Hitung F tabel

5% 1%

Ulangan 4 3,2634 x 10-4

Perlakuan 4 7,7664 x 10-4 Galad Acak 16 3,7581 x 10-5

Non Additivitas 1 7,0147 x 10-7 7,0147 x 10-7 0,2853 4,53 8,68 Sisa 15 3,6879 x 10-5 2,4587 x 10-7

F Hitung Non Additivitas < F Tabel 5% dan 1% Data Additif DAFTAR SIDIK RAGAM

Sumber Ragam Db JK KT F Hitung F tabel

5% 1%

Ulangan 4 3,2634 x 10-4 8,1584 x 10-5 34,7341** 3,01 4,77 Perlakuan 4 7,7664 x 10-4 1,9416 x 10-4 82,6628** 3,01 4,77 Galad Acak 16 3,7581 x 10-5 2,3488 x 10-6

Total 24

41 Lampiran 8 (Lanjutan)

KV = x 100% Uji Beda Nyata Jujur (BNJ) 5%

= x 100% BNJ 5 % : W = q α(p,n2)Sx

= 1,36 % Sx = = 6,8557 x10-4 BNJ 5 % = 4,34 x (5,7446 x 10-4)

= 2,9754 x 10-3 Aktivitas Antioksidan (mek / gram)

0%:100% 25%:75% 50%:50% 75%:25% 100%:0% Purata

± SE

0,1043 ± 0,0047

0,1084 ± 0,0071

0,1139 ± 0,0041

0,1166 ± 0,0047

0,1197 ± 0,0053

Lampiran 9. Analisa Data Kadar Fenolik Total Tempe

Ulangan Persentase Kedelai (Lokal : Impor) Tu Xu du W

100% L 75% L 50% L 25% L 100% I

1 5,0968 4,9718 4,8068 4,6686 4,4763 24,0203 4,8041 0,1022 0,249759 2 5,0454 4,892 4,7275 4,5349 4,2994 23,4992 4,6998 -0,0020 0,300054 3 4,6793 4,6117 4,5416 4,4537 4,165 22,4513 4,4903 -0,2116 0,202343 4 5,1142 4,9924 4,8399 4,7887 4,5848 24,3200 4,8640 0,1622 0,205574 5 4,9819 4,9173 4,7199 4,5385 4,0978 23,2554 4,6511 -0,0508 0,362071 Tp 24,9176 24,3852 23,6357 22,9844 21,6233 117,5462

Xp 4,9835 4,8770 4,7271 4,5969 4,3247 (X…) 4,7018 dp 0,2817 0,1752 0,0253 -0,1049 -0,3772

SD 0,1777 0,1537 0,1157 0,1319 0,2051 SE 0,2206 0,1909 0,1436 0,1639 0,2546

43 Lampiran 9 (Lanjutan)

W1= {(5,0968x 0,2817) + (4,9718x 0,1752) + (4,8068x0,0253) + (4,6686x-0,1049) + (4,4763x-0,3772)} = 0,249759

W2= {(5,0454x 0,2817) + (4,892x 0,1752) + (4,7275x0,0253) + (4,5349x-0,1049) + (4,2994x-0,3772)} = 0,300054

W3= {(4,6793x 0,2817) + (4,6117x 0,1752) + (4,5416x0,0253) + (4,4537x-0,1049) + (4,165x-0,3772)} = 0,202343

W4= {(5,1142x 0,2817) + (4,9924x 0,1752) + (4,8399x0,0253) + (4,7887x-0,1049) + (4,5848x-0,3772)} = 0,205574

W5= {(4,9819x 0,2817) + (4,9173x 0,1752) + (4,7199x0,0253) + (4,5385x-0,1049) + (4,0978x-0,3772)} = 0,362071

N = Wix du

={(0,249759 x0,1022) + (0,300054 x -0,0020) + (0,202343 x -0,2116) + (0,205574 x 0,1622) + (0,362071 x-0,0508) = -2,9348 x 10-3

D = du2x dp2

= {[(0,1022)2+ (-0,0020)2+ (-0,2116)2+ (0,1622)2 + (-0,0508)2]x [(0,2817)2 + (0,1752)2+ (0,0253)2+ (-0,1049)2+ (-0,3772)2]} = 22,1968 x 10-3

FK = = = 552,6844

JK Non Aditivitas = = = 3,8803 x 10-4

JK Total = p2– FK

= {(5,0968)2 + (4,9718)2+ …. +(4,0978)2} -552,6844 = 554,5165 - 552,6844

= 1,8321

JK Ulangan = - FK

Lampiran 9 (Lanjutan)

JK Perlakuan = - FK

= – 552,6844= 1,3198

JK Galad Acak = JK Total – JK Ulangan – JK Perlakuan = (1,8321) - (4,2046 x10-1)– (1,3198)

= 9,1861 x 10-2 DASIRA UJI ADDITIVITAS

Sumber Ragam Db JK KT F Hitung F tabel

5% 1%

Ulangan 4 4,2046 x 10-1

Perlakuan 4 1,3198

Galad Acak 16 9,1861 x 10-2

Non Additivitas 1 3,8803 x 10-4 3,8803 x 10-4 0,0636 4,53 8,68 Sisa 15 9,1473 x 10-2 6,0982 x 10-3

F Hitung Non Additivitas < F Tabel 5% dan 1% Data Additif DAFTAR SIDIK RAGAM

Sumber Ragam Db JK KT F Hitung F tabel

5% 1%

Ulangan 4 4,2046 x 10-1 0,1051 18,3085** 3,01 4,77

Perlakuan 4 1,3198 0,3299 57,4697** 3,01 4,77

Galad Acak 16 9,1861 x 10-2 0,0057

Total 24

46

KV = x 100% Uji Beda Nyata Jujur (BNJ) 5%

= x 100% BNJ 5 % : W = q α(p,n2)Sx

= 1,61 % Sx = = 3,3764 x10-2 BNJ 5 % = 4,34 x (3,6530 x 10-2)

= 0,1465

Kadar Fenolik Total (mg / gram)

0%:100% 25%:75% 50%:50% 75%:25% 100%:0% Purata

± SE

4,3247 ± 0,2546

4,5969 ± 0,1639

4,7271 ± 0,1436

4,8770 ± 0,1909

4,9835 ± 0,2206

JK Perlakuan = - FK

= – 31,6818 x 10-2 = 7,7664 x 10-4

JK Galad Acak = JK Total – JK Ulangan – JK Perlakuan

= (11,4056 x 10-4) - (3,2634 x 10-4)– (7,7664 x 10-4) = 3,7581 x 10-4

DASIRA UJI ADDITIVITAS

Sumber Ragam Db JK KT F Hitung F tabel

5% 1%

Ulangan 4 3,2634 x 10-4

Perlakuan 4 7,7664 x 10-4

Galad Acak 16 3,7581 x 10-5

Non Additivitas 1 7,0147 x 10-7 7,0147 x 10-7 0,2853 4,53 8,68

Sisa 15 3,6879 x 10-5 2,4587 x 10-7

F Hitung Non Additivitas < F Tabel 5% dan 1% Data Additif

DAFTAR SIDIK RAGAM

Sumber Ragam Db JK KT F Hitung F tabel

5% 1%

Ulangan 4 3,2634 x 10-4 8,1584 x 10-5 34,7341** 3,01 4,77 Perlakuan 4 7,7664 x 10-4 1,9416 x 10-4 82,6628** 3,01 4,77 Galad Acak 16 3,7581 x 10-5 2,3488 x 10-6

Lampiran 8 (Lanjutan)

KV = x 100% Uji Beda Nyata Jujur (BNJ) 5%

= x 100% BNJ 5 % : W = q α(p,n2)Sx

= 1,36 % Sx = = 6,8557 x10-4

BNJ 5 % = 4,34 x (5,7446 x 10-4) = 2,9754 x 10-3

Aktivitas Antioksidan (mek / gram)

0%:100% 25%:75% 50%:50% 75%:25% 100%:0% Purata

± SE

0,1043 ± 0,0047

0,1084 ± 0,0071

0,1139 ± 0,0041

0,1166 ± 0,0047

0,1197 ± 0,0053

Ulangan Persentase Kedelai (Lokal : Impor) Tu Xu du W 100% L 75% L 50% L 25% L 100% I

1 5,0968 4,9718 4,8068 4,6686 4,4763 24,0203 4,8041 0,1022 0,249759 2 5,0454 4,892 4,7275 4,5349 4,2994 23,4992 4,6998 -0,0020 0,300054 3 4,6793 4,6117 4,5416 4,4537 4,165 22,4513 4,4903 -0,2116 0,202343 4 5,1142 4,9924 4,8399 4,7887 4,5848 24,3200 4,8640 0,1622 0,205574 5 4,9819 4,9173 4,7199 4,5385 4,0978 23,2554 4,6511 -0,0508 0,362071 Tp 24,9176 24,3852 23,6357 22,9844 21,6233 117,5462

Xp 4,9835 4,8770 4,7271 4,5969 4,3247 (X…) 4,7018

dp 0,2817 0,1752 0,0253 -0,1049 -0,3772 SD 0,1777 0,1537 0,1157 0,1319 0,2051 SE 0,2206 0,1909 0,1436 0,1639 0,2546

Lampiran 9 (Lanjutan)

W1= {(5,0968x 0,2817) + (4,9718x 0,1752) + (4,8068x0,0253) + (4,6686x-0,1049) + (4,4763x-0,3772)} = 0,249759

W2= {(5,0454x 0,2817) + (4,892x 0,1752) + (4,7275x0,0253) + (4,5349x-0,1049) + (4,2994x-0,3772)} = 0,300054

W3= {(4,6793x 0,2817) + (4,6117x 0,1752) + (4,5416x0,0253) + (4,4537x-0,1049) + (4,165x-0,3772)} = 0,202343

W4= {(5,1142x 0,2817) + (4,9924x 0,1752) + (4,8399x0,0253) + (4,7887x-0,1049) + (4,5848x-0,3772)} = 0,205574

W5= {(4,9819x 0,2817) + (4,9173x 0,1752) + (4,7199x0,0253) + (4,5385x-0,1049) + (4,0978x-0,3772)} = 0,362071

N = Wix du

={(0,249759 x0,1022) + (0,300054 x -0,0020) + (0,202343 x -0,2116) + (0,205574 x 0,1622) + (0,362071 x-0,0508) = -2,9348 x 10-3

D = du2x dp2

= {[(0,1022)2+ (-0,0020)2+ (-0,2116)2+ (0,1622)2 + (-0,0508)2]x [(0,2817)2 + (0,1752)2+ (0,0253)2+ (-0,1049)2+ (-0,3772)2]} = 22,1968 x 10-3

FK = = = 552,6844

JK Non Aditivitas = = = 3,8803 x 10-4

JK Total = p2– FK

= {(5,0968)2 + (4,9718)2+ …. +(4,0978)2} -552,6844 = 554,5165 - 552,6844

= 1,8321

JK Perlakuan = - FK

= – 552,6844= 1,3198

JK Galad Acak = JK Total – JK Ulangan – JK Perlakuan = (1,8321) - (4,2046 x10-1)– (1,3198)

= 9,1861 x 10-2

DASIRA UJI ADDITIVITAS

Sumber Ragam Db JK KT F Hitung F tabel

5% 1%

Ulangan 4 4,2046 x 10-1

Perlakuan 4 1,3198

Galad Acak 16 9,1861 x 10-2

Non Additivitas 1 3,8803 x 10-4 3,8803 x 10-4 0,0636 4,53 8,68

Sisa 15 9,1473 x 10-2 6,0982 x 10-3

F Hitung Non Additivitas < F Tabel 5% dan 1% Data Additif

DAFTAR SIDIK RAGAM

Sumber Ragam Db JK KT F Hitung F tabel

5% 1%

Ulangan 4 4,2046 x 10-1 0,1051 18,3085** 3,01 4,77

Perlakuan 4 1,3198 0,3299 57,4697** 3,01 4,77

Galad Acak 16 9,1861 x 10-2 0,0057

KV = x 100% Uji Beda Nyata Jujur (BNJ) 5%

= x 100% BNJ 5 % : W = q α(p,n2)Sx

= 1,61 % Sx = = 3,3764 x10-2

BNJ 5 % = 4,34 x (3,6530 x 10-2) = 0,1465

Kadar Fenolik Total (mg / gram)

0%:100% 25%:75% 50%:50% 75%:25% 100%:0% Purata

± SE

4,3247 ± 0,2546

4,5969 ± 0,1639

4,7271 ± 0,1436

4,8770 ± 0,1909

4,9835 ± 0,2206