Analisis Keragaman Beberapa Genotipe Tanaman Tebu (Saccharum spp.) Di Sumatera Utara Berdasarkan Marka RAPD (Random Amplified Polymorphism DNA)

  Ahmed, A.Z.dan K. A. M. Khaled.2008. Detection of genetic similarity of sugarcane genotypes. sugar crops res. Inst.Agric. Res. Center, Giza, Egypt. Anderson J.M., 2000. Strategies of photosynthetic adaptations and acclimation. dalam: Yunus M., Pathre U., Mohanty P., editor. probing photosynthesis. mechanisms, regulation, and adaptation. London: Taylor & Francis. pp. 284-291. Anonim. 2005. Menghijaukan pantai memanen minyak.http://wwwfaperta.ugm.ac.id/jatropha. Diakses pada 22 Mei 2014. Balitbangtan. 2007. Prospek dan arah pengembangan agribisnis tebu.edisi ke dua.

  Badan Penelitian dan Pengembangan Pertanian, Departemen Pertanian. Bonnett G.D., Nowak E., Olivares-Villegas J.J., Berding N., Morgan T.,Aitken

  K.S. 2008. Identifying the risks of transgene escapefrom sugarcane crops to related species, with particularreference to Saccharum spontaneum.

  Trop. Plant Biol. 1:58–71 Crowder L.V. 2006. Genetika Tumbuhan. Diterjemahkan oleh Lilik Kusdiarti.

  Gadjah Mada University Press. Yogyakarta. 499 hlm. Daniels, J. and Daniels C.A. 1975. Geographical, historical and cultural aspects of theorigin of the indian and chinese sugarcanes S. barberi and S. sinense.

  SugarcaneBreeding Newsletters, Vol. 36, pp. 4–23. Darke R. 1999. The color encyclopedia of ornamental grasses: sedges,rushes, restios, cat-tails and selected bamboos. Timber Press,Portland.

  Delita, K., Efriani, M. dan Ummi, K. 2008. Korelasi aktivitas nitrat reduktasedan pertumbuhan beberapa genotipe tanaman jarak pagar (Jatrophacurcas

  

Linn.) yang diperlakukan dengan zat pengatur tumbuh 2,4-D.Jurnal Akta

Agrosia 11 (1) : 80 – 86.

  Departemen Pertanian, 2013. http://ditjenbun.deptan.go.id/bbpptpmedan/statis-6- visimisi.html diakses 28 Desember 2013. Direktorat Jenderal Perkebunan. 2007. Permentan 37, 38, 39. Departemen Pertanian. Jakarta. 42 hlm. Daniels, J., Roach, B. T. 1987. “Taxonomy And Evolution”. InD’Heinz (Eds). sugarcane improvement throughbreeding. Development In Crop Science II. Elsevier.Hlm 9. Filgueiras T.S. dan Lerina R. 2001. Saccharum. In: Longhi-Wagner, HM(ed) poacaeae, flora fanerogâmica do estado de são paulo,fapesp-hucitec, são paulo. http://www.ibot.sp.gov.br/PESQUISA/florasp/florasp.htm. Diakses 29 Januari 2014. Farooq, S; dan Azam, F. 2002. Molecular markers in plantbreeding – 1: concepts and characterization. Pakistan journal of biological sciences 5 (10): 1135-

  1140. Gorham, J., A. Bristol, E.M. Young dan R.G. Wyn Jones. 1991. The presence of

  • the enhanced K /Na discrimination trait in diploid Triticum species.

  Theoretical and Applied Genetics, 82:729-736.

  Hemmat, M, Weeden, N.F, Managanaris, A.G, Lawson, DM. 1994. Molecular marker linkage map for apple J. Heredity. 85:4-11 Hussain, A. 2003. Isolation, purification and characterization of invertases from sugarcane grown under saline condition. Thesis. Faculty of Science

  University of Agriculture. Faisalabad, Pakistan. Hussain K., M. F. Nisar, K. Nawaz, A. Majeed, andK.H. Bhatti.2010.

  Morphological traits vs. genetic diversity: reliable basisfor sugarcane varieties identification. The BIOL (E-Journal of Life Sciences) 1(2): 41-43. Indrawanto.C., Purwono, Siswanto, M.Syakir dan W. Rumini M.S., 2010.

  Budidaya dan Pasca Panen Tebu. Pusat Penelitian dan Pengembangan Perkebunan. ESKA Media. Jakarta. Indriani, F. C. 2000. Keragaman genetik plasma nutfah kenaf (hibiscuscannabinus l.) dan beberapa spesies yang sekerabat berdasarkananalisis isozim. Tesis.

  Fakultas Pertanian. Program Pasca Sarjana.Universitas Brawijaya. Malang. Joshi, S.P., Prabhakar K., Ranjekar, P.K and Gupta, V.S. 2011. Molecular markers in plant genome analysis. http/www.ias.ac.in/currsci/jul25/articles

  15. htm. Julisaniah, N.I., Sulistyowati, L., dan Sugiharto, A.N. 2008. Analisis kekerabatan mentimun(Cucumis sativus L.) menggunakan Metode RAPD – PCR danIsozim. Jurnal Biodiversitas 9 (2) : 99 – 102 Kartikaningrum, S., Hermiati, N., Baihaki, A., Murdaningsih, H. K., dan Nurita,T.

  M. 2002. Kekerabatan antar genus anggrek sub tribe sarchantinaeberdasarkan data fenotip dan pola pita DNA. Jurnal Zuriat 13 (1) : 1 –10. Kawar P.G., Devarumath P.M., Nerkar Y. 2009. Use RAPD marker for assement of genetic diversity in sugarcane cultivars. International Journal of

  Biotechnology 8, 67-71. Kementerian Pertanian, 2012. Laporan kinerja kementerian pertanian tahun 2011. www.deptan.go.id. Diakses pada tanggal 28 Januari 2014.

  Kiss, G B., Osanandi G., Kalman K, Kalo P, Okresz L. 1993. Construction of a basic linkage map of alfalfa using RFLP. RAPD, isozyme and morphological markers. Mol. Gen. Genet. 238:129- 137

  Karuniawan, A., Sahala, B., dan Ismail, A. 2008. Keanekaragaman genetikmucuna berdasarkan karakter morfologi dan komponen hasil.

  JurnalZuriat 19 (1) : 41 – 59.

  Liu, C.J., Witcombe, J.R., Pittawy T.S., Nash, M, Hash C.T., Brusso, C.S. dan Gale, M.D.1994. An RFLP-based genetic map of pearl millet (Pennisetum

  glaucum). Theoretical and applied genetics 8:481-487 dalam P.M Jonah,

  L. L. Bello, O. Lucky, A. Midau, S. M. Moruppa.2011. The importance of molecular markers in plant breeding programmes. Global Journal of Science Frontier Research Vol. 11. Global Journals Inc. (USA). Lynch M. dan B.G. Milligan.1994. Analysis of population genetic structure with rapd markers. Molecular Ecology (1994) 3, 91-99.

  Maftuchah, 2001. Strategi pemanfaatan penanda molekuler dalam perkembangan bidang hortikultura. Makalah sarasehan pemanfaatan pendanda molekuler di bidang hortikultura. Perhorti Jatim-deptan. Mansyah, E., Baihaki, A., Setiamiharja, R., Darsa, J. S., dan Sobir. 2003.

  Analisisvariabilitas genetik manggis (Garcinia mangostana L.) di Jawa danSumatra Barat menggunakan teknik RAPD. Jurnal Zuriat 14 (1) : 35 –

  44. Melchinger, A.E., 1999. Genetic diversity and heterosis, The genetics and exploitation of heterosis incrops, Coors. J.G. and Pandey S. (ed). American Society of Agronomy, Crop Science Society ofAmerica, and Soil Science Society of America, Madison, WI. Mullis, K. B., dan F. A. Faloona. 1987. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 155 :335-350. Mulyadi M.,Rusjidi dan D. Siahaan. 1997. Pengenalan Varietas Tanaman Tebu.

  Balai Penelitian Tembakau Deli PTP. Nusantara II. Medan. McMurdie P.J. dan S. Holmes. 2013. phyloseq: An R package for reproducible interactiveanalysis and graphics of microbiome census data. Plos One.

  Vol. 8 : 1-11. Nasir, M., 2002. Bioteknologi Molekuler. PT. Citra Aditya Bakti, Bandung. 286 hlm.

  , 2002. Bioteknologi. Potensi dan Keberhasilannya Dalam Bidang Pertanian. PT.

  Raja Grafindo Persada. Jakarta. 286 hlm. Oldeman, L.R.; and D. Syarifuddin. 1977. An Agroclimatic Map of Sulawesi.

  Central Research Institute for Agriculture, Bogor, Indonesia. Orozco-Castillo C., Chalmers K. J., Waugh R. dan Powell, 1994. Detection of genetic diversity and selective gene introgression in coffee using RAPD marker.Theor.Appl.Genet. 87: 934-940. Pandey R.N., S.P. Singh, J. Rastogi, M.L. Sharma and R.K. Singh.2012. Early assessment of genetic fidelity in sugarcane (Saccharum officinarum) plantlets regenerated through direct organogenesis with RAPD and SSR markers. AJCS 6(4):618-624.

  Perrier X. dan Jacquemoud-Colled J.P.,2006. DARwin Software.http://darwin.cirad.fr/darwin

  Poerba, Y. S. dan Martanti, D. 2008. Keragaman genetik berdasarkan markarandom amplified polymorphic DNA pada Amorphopallus

  muelleriBlume di Jawa. Jurnal Biodiversitas 9 (4) : 245 – 249.

  Rahman, M. and Y. Zafar. 2001. Genotyping of a new strain of lentil (Lens culinaris Medik.) by DNA fingerprinting technology. Pakistan Journal of

  Botany, 33(4): 423-428.

  Rahman, M.; T.A. Malik; M.A.Chowdhary; M.J. Iqbal andY.Zafar. 2004.

  Application of random amplified polymorphic DNA (RAPD) technique for the identification of markers linked to salinity tolerance in wheat (Triticum aestivum L.). National Institute for Biotechnology and Genetic Engineering (NIBGE). Department of Plant Breeding & Genetics, University of Agriculture Faisalabad, Pakistan. Department of Plant, Soil and General Agriculture, Southern Illinois University, Carbondale, IL 62901, USA. Restu. M, Mukrimin dan Gusmiaty. 2012. Optimalisasi teknik ekstraksi dan isolasi tanaman suren (Toona Sureni Merr.) untuk analisis keragaman genetik berdasarkanrandom amplified polymorphic DNA (RAPD). Fakultas Kehutanan, Universitas Hasanuddin, Makassar. Dalam Jurnal Natur Indonesia 14(2), Februari 2012: 138-142.

  Risbang Tebu, 2013. Data KBI 2014/2015. PTP. Nusantara II. Medan. Saitou, N. dan M. Nei, 1987. The neighbor – joining method : A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4 (4) : 406 – 425.

  Salisburry, F.B dan C.W. Ross. 1992. Fisiologi Tumbuhan Jilid 3: Biokimia Tumbuhan. D.R. Lukman dan Sumargono (Penerjemah). Penerbit ITP.

  Bandung. 173 Hal. Terjemahan dari: Plant Physiology:Plant Biochemistry. Saragih, D.Y. 2013. Keragaman molekuler dan pengaruh naungan terhadap beberapa mutan kedelai (Glycine max L.) hasil mutasi kolkisin. Tesis.

  Universitas Sumatera Utara. Medan. Sinaga, R., E. Susanto. 2009. Ekplorasi varietas tebu (Saccharum officinarum) toleran terhadap kekeringan di sumatera utara berdasarkan respon morfofisiologi. Laporan Hasil Penelitian. Hibah Penelitian Strategis Nasional. Universitas Sumatera Utara. Medan.

  Setiyo I.E. 2001. Pemetaan dan keragaman genetik RAPD pada kelapa sawit sungai pancur (RISPA). Tesis. Institut Pertanian Bogor. Program Pascasarjana (tidak dipublikasikan). 47 hlm. Scortecci, K.C, S.Creste, T. Calsa Jr., M. A. Xavier. 2012. Challenges, opportunities and recentadvances in sugarcane breeding. http://www.intechopen.com/books/plant-breeding. Diakses pada tanggal 22 Januari 2014.

  Sreenivasan T.V., Ahloowalia B.S., Heinz D.J. 1987. Cytogenetics. In:Heinz D.J.

  (ed) Sugarcane Improvement Through Breeding.Elsevier, Amsterdam, pp 211–254. Smith L.B., Wasshausen D.C., Klein R.M..1982. Gramíneas. Gêneros:85.

  Paspalum até Zea. Flora Ilustrada Catarinense. HerbárioBarbosa Rodrigues, Itajaí, p. 504. Stammers M., Harris J., Evans G.M., Hayward M.D., et al. 1995. Use of random

  PCR (RAPD) technology to analyse phylogenetic relationships in the Lolium/Festuca complex. Heredity 74 (Pt 1): 19-27. Tabasum S, F.A. Khan, S. Nawaz, M.Z. Iqbal andA. Saeed. DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA.

  Genetics and Molecular Research 9 (1): 471-483.

  Tim Penyusun, 2010. Kamus Pertanian Umum. Penebar Swadaya. Jakarta.

  535 hlm. Toharisman, A.2007. Kinerja industri gula indonesia 2007. Pusat Penelitian Perkebunan Gula Indonesia (P3GI) – Pasuruan.

  Toruan, N. Mathius, D. Rahmawati dan Anidah. 2009. Genetic Variation among

  Aquilaria Species and Gyrinops Versteegiiusing amplified fragment length polymorphism markers. Biotropia vol. 16 no.2 : 88 – 95. Ullah S.M.S. Md. Amzad Hossain, Md. M. Hossain, S. Barman, Md. M. Hasan, Sohag, S. H. Prodhan. 2013.Genetic diversity analysis of chewing sugarcane (Saccharum spp.) varieties by using RAPD markers.J. BioSci.

  Biotech. 2013, 2(2): 145-150. ISSN: 1314-6246.

  Webster R, Shaw RB.1995. Taxonomy of the native north Americanspecies of Saccharum (Poaceae:Andropogoneae). Sida 16(3):551–580

  ZhangX.Q., X. Ma, X.Y. Gu, T.T. Chen, S.Y. Chen, L.-K. Huang.2013. Genetic relationships between Lolium (Poaceae) species revealed by RAPD markers. Genetics and Molecular Research 12 (3): 3246-3255. Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski, dan S.V. Tingey. 1990.

  DNA polymorphism amplified by arbitraryprimers are useful as genetic markers. Nucleic Acids. Res. 18: 6531.6535dalamP.M Jonah, L. L. Bello, O. Lucky, A. Midau, S. M. Moruppa.2011. The Importance of Molecular Markers in Plant Breeding Programmes. Global Journal of Science Frontier Research Vol. 11. Global Journals Inc. (USA).

  Wikipedia, 2013. Genotipe. Diakses pada tanggal 24 Nopember 2013. Welsh J. dan McClelland M.1990. Fingerprinting genomes using PCR with arbitrary primers. California Institute of Biological Research California

  Institute of Biological Research. USA. Nucleic Acids Research, Vol. 18, No. 24. Oxford University Press . 7213-7218. Yunita, R. 2009. Pemanfaatan variasi somaklonal dan seleksi in vitro dalam perakitan tanaman toleran cekaman abiotik. Balai Besar Penelitian dan

  Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Kementerian Pertanian. Bogor.