Abstract Pengaruh Pemberian Jus Buah Delima (Punica Granatum L.) Terhadap Kualitas Sperma Pada Mencit Yang Telah Diinduksi Ekstrak Daun Tembakau
PENGARUH PEMBERIAN JUS BUAH DELIMA (Punica granatumL.)
TERHADAP KUALITAS SPERMA MENCIT YANG TELAH DIINDUKSI
EKSTRAK DAUN TEMBAKAU
ABSTRAK
Ekstrak daun tembakau menyebabkan terjadinya penurunan kualitas spermatozoa.
Penurunan kualitas spermatozoa dapat dipulihkan dengan pemberian Jus Buah
Delima. Penelitian ini memakai mencit jantan (Mus musculus L.) dewasa fertil
berumur ± 2 bulan. Mencit dengan berat badan 20-22 gram sebanyak 24ekor.
Mencit dibagi menjadi 3 kontrol dan 3 perlakuan. Masing-masing terdiri dari 5
ekor. Kontrol 0 adalah kontrol negatif yang hanya diberi akuades, kontrol 1 adalah
kontrol positif yang diberi ekstrak daun tembakau dengan dosis
0,121mg/0,1ml//hari/ mencit dan kontrol 2 adalah kontrol positif yang diberi jus
buah delima dengan dosis 0,1 ml/hari/mencit. Pada perlakuan 1, 2 dan 3
pemberian ekstrak daun tembakau dengan dosis 0,121mg/0.1ml/hari/mencit dan
jus buah delima dengan konsentrasi 4%, 5%, 6% sebanyak 0.1ml/mencit selama
35 hari melalui oral. Proses pemulihan dilakukan pada kontrol 2, perlakuan 1, 2
dan 3 yang diberikan Jus buah delima sebanyak 0,1mg/hari/mencit. Pembedahan
dilakukan setelah 35 hari melalui dislokasi leher. Pemeriksaan spermatozoa
menggunakan kamar hitung Improved Neubauer dan mikroskop cahaya. Hasil
penelitian didapat jus buah delima dapat meningkatkan kualitas sperma mencit
yang turun akibat ekstrak daun tembakau.
Kata kunci: Ekstrak daun tembakau, Jus buah delima, Mus musculus L.,
Spermatozoa
v
GIVING EFFECT OF FRUIT JUICE POMENGRANATE (Punica granatum
L.) THE QUALITY OF SPERM ON MICE WHICH HAVE INDUCED
TOBACCO EXTRACT
ABSTRACT
Tobacco leaf extract led to a decline in sperm quality. Decline in sperm quality
can be restored by administration of Pomegranate Juice. The study used male
mice (Mus musculus L.) grown in fertile age ± 2 months. Mice weighing 20-22
grams were 24 tails. Mice were divided into 3 control and 3 treatment. Each
consisting of 5 mice. Control 0 is the negative control which was only given
distilled water, 1 control was positive controls were given extracts of tobacco
leaves with a dose of 0,121mg/day/0,1ml mice and control 2 are positive controls
were given pomegranate juice at a dose of 0.1 ml /day/ mice. In the treatment 1, 2
and 3 of extract of tobacco leaves with a dose of 0,121mg /0.1ml /day /mice and
pomegranate juice with a concentration of 4%, 5%, 6% as much as 0.1ml / mice
for 35 days orally. The recovery process is done in the control 2, treatment of 1, 2
and 3 were given pomegranate juice as much as 0.1 mg /day/mice. Surgery is
performed after 35 days by cervical dislocation. Examination of spermatozoa
using the Improved Neubauer counting chamber and the light microscope. The
result is pomegranate juice may improve sperm quality in mice which fell due to
tobacco leaf extracts.
Keywords: Pomegranate juice,Spermatozoa, Tobacco extract, Mus musculus L.
vi
TERHADAP KUALITAS SPERMA MENCIT YANG TELAH DIINDUKSI
EKSTRAK DAUN TEMBAKAU
ABSTRAK
Ekstrak daun tembakau menyebabkan terjadinya penurunan kualitas spermatozoa.
Penurunan kualitas spermatozoa dapat dipulihkan dengan pemberian Jus Buah
Delima. Penelitian ini memakai mencit jantan (Mus musculus L.) dewasa fertil
berumur ± 2 bulan. Mencit dengan berat badan 20-22 gram sebanyak 24ekor.
Mencit dibagi menjadi 3 kontrol dan 3 perlakuan. Masing-masing terdiri dari 5
ekor. Kontrol 0 adalah kontrol negatif yang hanya diberi akuades, kontrol 1 adalah
kontrol positif yang diberi ekstrak daun tembakau dengan dosis
0,121mg/0,1ml//hari/ mencit dan kontrol 2 adalah kontrol positif yang diberi jus
buah delima dengan dosis 0,1 ml/hari/mencit. Pada perlakuan 1, 2 dan 3
pemberian ekstrak daun tembakau dengan dosis 0,121mg/0.1ml/hari/mencit dan
jus buah delima dengan konsentrasi 4%, 5%, 6% sebanyak 0.1ml/mencit selama
35 hari melalui oral. Proses pemulihan dilakukan pada kontrol 2, perlakuan 1, 2
dan 3 yang diberikan Jus buah delima sebanyak 0,1mg/hari/mencit. Pembedahan
dilakukan setelah 35 hari melalui dislokasi leher. Pemeriksaan spermatozoa
menggunakan kamar hitung Improved Neubauer dan mikroskop cahaya. Hasil
penelitian didapat jus buah delima dapat meningkatkan kualitas sperma mencit
yang turun akibat ekstrak daun tembakau.
Kata kunci: Ekstrak daun tembakau, Jus buah delima, Mus musculus L.,
Spermatozoa
v
GIVING EFFECT OF FRUIT JUICE POMENGRANATE (Punica granatum
L.) THE QUALITY OF SPERM ON MICE WHICH HAVE INDUCED
TOBACCO EXTRACT
ABSTRACT
Tobacco leaf extract led to a decline in sperm quality. Decline in sperm quality
can be restored by administration of Pomegranate Juice. The study used male
mice (Mus musculus L.) grown in fertile age ± 2 months. Mice weighing 20-22
grams were 24 tails. Mice were divided into 3 control and 3 treatment. Each
consisting of 5 mice. Control 0 is the negative control which was only given
distilled water, 1 control was positive controls were given extracts of tobacco
leaves with a dose of 0,121mg/day/0,1ml mice and control 2 are positive controls
were given pomegranate juice at a dose of 0.1 ml /day/ mice. In the treatment 1, 2
and 3 of extract of tobacco leaves with a dose of 0,121mg /0.1ml /day /mice and
pomegranate juice with a concentration of 4%, 5%, 6% as much as 0.1ml / mice
for 35 days orally. The recovery process is done in the control 2, treatment of 1, 2
and 3 were given pomegranate juice as much as 0.1 mg /day/mice. Surgery is
performed after 35 days by cervical dislocation. Examination of spermatozoa
using the Improved Neubauer counting chamber and the light microscope. The
result is pomegranate juice may improve sperm quality in mice which fell due to
tobacco leaf extracts.
Keywords: Pomegranate juice,Spermatozoa, Tobacco extract, Mus musculus L.
vi