Uji Antibakteri Ekstrak Air Bawang Putih (Allium Sativum) dan Hasil Hidrolisis Enzimatis Minyak Kelapa Murni serta Kombinasinya terhadap Beberapa Bakteri Penyebab Diare
Lampiran 1. Hasil identifikasi bawang putih
Lampiran 2. Bahan uji VCO dan bawang putih Keterangan: A. Minyak Kelapa Murni (VCO); B. Bawang putih
A B
®
Lampiran 3. Tris (hidroksil) amninometana dan Lipozyme TL IM A B
®
Keterangan: A. Tris (hidroksil) amninometana (Merck); B. Lipozyme TL IM
Lampiran 4. Bahan untuk pengujian antibakteri
Keterangan: A. Nutrient Agar (OXOID); B. Mueller Hinton Agar (OXOID); C.
NaCl 0,9%; D. Aqua bidestilata steril; E. Pencadang kertas diameter 6 mm (OXOID) A B C D
E
Lampiran 5. Sertifikat pengujian Tetrasiklin Hidroklorida
Lampiran 6. Bagan kerja hidrolisis VCO secara enzimatik 30 g VCO Dimasukkan ke dalam erlenmeyer
Ditambahkan 50 ml akuades, 12,5 ml
CaCl
2 0,063 M, 25 ml larutan buffer
Tris-HCl, dan 500 mg enzim
®
LIPOZIM TL IM Diaduk dengan batang pengaduk 10
menit
Diinkubasi selama 14 jam pada suhuo
55±0,5
C, sambil dikocok setiap jam selama 10 menit Dipindahkan ke dalam corong pisah untuk diekstraksi dengan 50 ml n-heksan Lapisan bawah Lapisan atas (n-heksan)
(air) Filtrat I Diekstraksi lagi dengan 50 ml n-heksan Lapisan bawah Lapisan atas (n-heksan)
(air) Filtrat II Digabung Ditambahkan 50 mg Na
2 SO 4 anhidrat
Didiamkan selama 15 menit Disaring Diuapkan di atas penangas air untuk menghilangkan n-heksan
Hasil hidrolisis VCO
Lampiran 7. Jumlah bahan untuk hidrolisis VCO Buffer CaCl
2 Enzim
No. Berat VCO Akuades Tris- ® n-heksan Na
2 SO
4
0,063 M LIPOZIM
Hidrolisis (g) (ml) HCl (ml) (mg)
(ml) TL IM (mg) (ml)1 30,0133 50 12,5 25 501,0112 100
50 2 30,5002 50 12,5 25 502,1002 100
50 3 31,0070 50 12,5 25 501,7022 100
50 Pembuatan larutan CaCl
2 0,063 M 50 ml dan buffer Tris-HCl:
1. Larutan CaCl
2 0,063 M 50 ml
Timbang 463,1 mg CaCl .2H O, tambahkan akuades hinggal 50 ml.
2
2
mg M = BM × ml mg = 0,063 × 111 × 50 ml = 463,1 mg
2. Larutan buffer Tris-HCl 100 ml Larutkan Tris (hidroksil) amninometana 12,11 gram dalam akuades 80 ml HCl 4,2 ml. Atur pH 8, dan add dengan akuades hingga volume 100 ml.
(p)
Lampiran 8. Pembakuan KOH yang diperlukan untuk penentuan bilangan asam Miligrek Kalium Biftalat=Miligrek KOH mg K.Biftalat
BE =V ×N KOH N KOH= mg K Biftalat
BE ×V Keterangan: BE K.Biftalat = bobot ekuivalen = 204,2 V = volume titrasi KOH N = normalitas
Normalitas rata-rata: N �i= N1 + N2
2 Deviasi (%): D =
� Ni + N � N
� � × 100% mg
KBiftalat BE KBiftalat Vol titrasi
(ml) N KOH N �i
D (%) Pembakuan I 101,0021 204,2 5,25 0,0942 0,0949 1,58
100,7011 204,2 5,15 0,0957 0,0938 0,85 100,1751 204,2 5,25 0,0934 0,0945 2,43 Pembakuan II 102,0017 204,2 5,20 0,0961 0,0942 3,81
101,0991 204,2 5,35 0,0925 0,0954 1,36 101,022 204,2 5,25 0,0948 0,0936 2,45 Pembakuan III 103,0021 204,2 5,15 0,0979 0,0974 1,02
102,9211 204,2 5,20 0,0969 0,0979 0,10 103,0713 204,2 5,15 0,0980 0,0974 1,12 Kesimpulan: N KOH I = 0,0938 N N KOH II = 0,0954 N
N KOH III = 0,0979 N
Lampiran 9. Perhitungan bilangan asam Bilangan Asam (acid value) =
II 1 5,1021 56,1 0,0938 132,05 136,1931 138,8796 ± 2,6029
VCOT1 3 .3865 .4913 .435100 .0528117 HVCO1 3 136.0174 141.5228 138.667967 2.7583783 HVCO2 3 136.1931 141.3900 138.879633 2.6029250 HVCO3 3 136.3949 138.5888 137.292700 1.1499050 Valid N (listwise)
Deviation
N Minimum Maximum Mean Std.
2 5,0021 56,1 0,0954 130,75 139,8944 3 5,1003 56,1 0,0979 128,70 138,5888 Descriptive Statistics
III 1 5,0579 56,1 0,0938 131,10 136,3949 137,2927 ± 1,1499
2 5,0900 56,1 0,0954 132,25 139,0558 3 5,1255 56,1 0,0979 131,95 141,3900 HVCO
2 5,1021 56,1 0,0954 132,00 138,4637 3 5,0780 56,1 0,0979 130,85 141,5228 HVCO
A × N ×BM KOH G Keterangan: A = jumlah ml KOH untuk titrasi N = normalitas larutan KOH G = bobot minyak (gram) BM KOH = 56,1 Sampel n
I 1 5,0700 56,1 0,0938 131,05 136,0174 138,6679 ± 2,7583
2 5,0072 56,1 0,0954 0,40 0,4275 3 5,0301 56,1 0,0979 0,45 0,4913 HVCO
VCOT 1 4,9970 56,1 0,0938 0,35 0,3865 0,4351 ± 0,0528
Asam Mean ± SD
(ml) Bil.
KOH N KOH Volume Titrasi
Berat Minyak (g) BM
3
Lampiran 10. Zona hambat HVCO dan EABP dalam akuades serta VCOT Bahan uji Kadar
(v/v) Zona hambat (mm)
BC SA EC ST SD
VC Blanko (akuades) - - - - - -
VCOT 100% 7,0 6,2 6,4 6,1 - 6,2 HVCO 10% - - - - 6,2 - 25% 6,1 7,5 - 7,5 7,0 6,5 50% 7,9 8,9 7,2 8,9 7,9 6,9 75% 8,1 10,7 8,7 9,3 8,7 7,8 100% 10,5 11,2 9,0 9,4 9,4 8,5 EABP 10% 13,0 9,3 9,6 - 7,0 8,0 25% 17,0 16,7 11,5 6,1 9,5 9,3 50% 17,9 16,0 13,4 8,1 11,0 11,3 75% 19,5 16,5 15,5 9,4 12,5 12,4 100% 23,8 18,2 20,0 19,5 19,6 19,0 HVCO 50% + EABP 50% 1:1 12,5 11,5 9,4 8,7 9,4 10,0
HVCO 75% + EABP 25% 3:1 11,0 8,5 8,0 6,7 9,0 8,5 HVCO 25% + EABP 75% 1:3 16,1 14,0 11,7 9,1 9,7 12,6
VCOT 50% + EABP 50% 1:1 15,0 9,8 10,4 8,0 10,6 12,4
VCOT 75% + EABP 25% 3:1 13,0 6,5 8,1 7,0 7,5 10,5
VCOT 25% + EABP 75% 1:3 17,2 13,2 13,0 12,1 14,3 14,9
Keterangan: diameter pencadang kertas 6 mm; (-): tidak ada zona hambat; BC:
Bacillus cereus ATCC 14579; SA: Staphylococcus aureus ATCC 25923; EC: Escherichia coli ATCC 8939; ST: Salmonella thypi ATCC 00786; SD: Shigella dysenteriae ATCC 13313; VC: Vibrio cholera ATCC 39315Lampiran 11. Gambar hasil uji antibakteri HVCO dan EABP dalam akuades serta VCOT terhadap Bacillus cereus ATCC 14579
Lampiran 12. Gambar hasil uji antibakteri HVCO dan EABP dalam akuades serta VCOT terhadap Staphylococcus aureus ATCC 25923
Lampiran 13. Gambar hasil uji antibakteri HVCO dan EABP dalam akuades serta VCOT terhadap Escherichia coli ATCC 8939
Lampiran 14. Gambar hasil uji antibakteri HVCO dan EABP dalam akuades serta VCOT terhadap Salmonella thypi ATCC 00786
Lampiran 15. Gambar hasil uji antibakteri HVCO dan EABP dalam akuades serta VCOT terhadap Shigella dysenteriae ATCC 13313
Lampiran 16. Gambar hasil uji antibakteri HVCO dan EABP dalam akuades serta VCOT terhadap Vibrio cholera ATCC 39315
Lampiran 17. Zona hambat VCOT, HVCO, dan EABP dalam etanol Bahan uji Kadar
(v/v) Zona hambat (mm)
BC SA EC ST SD
VC Blanko (etanol) - - - - - -
VCOT 10% - - - - - - 25% - 6,3 - - - - 50% - 6,3 - - - - 75% 6,2 6,8 - - - - 100% 7,4 10,9 7,9 6,7 - 6,4
HVCO 10% 8,3 8,6 8,0 7,6 8,4 8,1 25% 8,5 9,7 8,7 7,9 9,5 9,4 50% 9,4 9,5 9,4 9,0 10,2 9,5 75% 10,9 10,5 9,5 9,7 10,0 10,6 100% 13,7 14,2 10,7 11,8 12,3 12,7
Blanko (etanol+akuades 1:1) 7,9 7,0 6,2 6,4 7,0 6,5 EABP 10% 12,6 8,5 - - 7,2 7,8 25% 15,9 8,6 - - 7,0 10,1 50% 17,4 9,0 8,3 10,0 7,7 13,2 75% 18,3 9,2 10,0 10,5 8,5 15,0 100% 18,9 10,0 11,2 11,0 9,6 14,1 HVCO 50% + EABP 50% 1:1 19,0 17,3 19,1 12,2 10,5 17,0
HVCO 75% + EABP 25% 3:1 13,4 16,0 15,2 11,0 9,2 12,2 HVCO 25% + EABP 75% 1:3 22,5 21,1 18,0 20,2 11,2 20,6
VCOT 50% + EABP 50% 1:1 16,9 15,9 19,4 18,3 9,0 16,6
VCOT 75% + EABP 25% 3:1 16,0 12,0 13,4 10,0 7,4 15,0
VCOT 25% + EABP 75% 1:3 20,7 16,0 19,0 20,9 11,0 22,0
Keterangan: diameter pencadang kertas 6 mm; (-): tidak ada zona hambat; BC:
Bacillus cereus ATCC 14579; SA: Staphylococcus aureus ATCC 25923; EC: Escherichia coli ATCC 8939; ST: Salmonella thypi ATCC 00786; SD: Shigella dysenteriae ATCC 13313; VC: Vibrio cholera ATCC 39315Lampiran 18. Gambar hasil uji antibakteri VCOT, HVCO, dan EABP dalam etanol terhadap Bacillus cereus ATCC 14579
Lampiran 19. Gambar hasil uji antibakteri VCOT, HVCO, dan EABP dalam etanol terhadap Staphylococcus aureus ATCC 25923
Lampiran 20. Gambar hasil uji antibakteri VCOT, HVCO, dan EABP dalam etanol terhadap Escherichia coli ATCC 8939
Lampiran 21. Gambar hasil uji antibakteri VCOT, HVCO, dan EABP dalam etanol terhadap Salmonella thypi ATCC 00786
Lampiran 22. Gambar hasil uji antibakteri VCOT, HVCO, dan EABP dalam etanol terhadap Shigella dysenteriae ATCC 13313
Lampiran 23. Gambar hasil uji antibakteri VCOT, HVCO, dan EABP dalam etanol terhadap Vibrio cholera ATCC 39315
Lampiran 24. Diagram perbandingan aktivitas antibakteri menggunakan pelarut akuades, etanol 96% dan DMSO terhadap Staphylococcus aureus ATCC 25923, Salmonella thypi ATCC 00786, Shigella dysenteriae ATCC 13313, dan Vibrio cholera ATCC 39315
18
Salmonella thypi ATCC 00786 Akuades Etanol 96% DMSO Akuades+etanol 1:1 Akuades+DMSO 1:1
20
15
10
5
2
Staphylococcus aureus ATCC 25923 Akuades Etanol 96% DMSO Akuades+etanol 1:1 Akuades+DMSO 1:1
20 Blanko
16
14
12
10
8
6
4
VCOT 100% HVCO 100% EABP 100%
25 Blanko
VCOT 100% HVCO 100% EABP 100%
Lampiran 24. (Lanjutan)
25 Blanko
VCOT 100% HVCO 100% EABP 100%
20 Blanko
12
Vibrio cholera ATCC 39315 Akuades Etanol 96% DMSO Akuades+etanol 1:1 Akuades+DMSO 1:1
18
16
14
10
5
8
6
4
2
Shigella dysenteriae ATCC 13313 Akuades Etanol 96% DMSO Akuades+etanol 1:1 Akuades+DMSO 1:1
20
15
10
VCOT 100% HVCO 100% EABP 100%
Lampiran 25. Data pengukuran zona hambat terhadap Bacillus cereus ATCC 14579 Bahan uji
VCOT 50% + EABP 50% 1:1 17,6 14,3 15,4 15,6 16,0
5 Blanko (akuades) - - - - - Tetrasiklin HCl 5 29,3 29,0 29,8 28,9 27,4 2,5 27,4 26,9 27,5 27,7 27,7 1 27,0 27,3 26,9 27,5 26,8 0,5 24,4 24,9 24,7 24,7 24,9
4
3
2
1
Zona hambat (mm) Pengulangan ke-
VCOT 25% + EABP 75% 1:3 18,0 18,8 19,0 19,1 18,6 Baku pembanding Kadar (mg/ml)
VCOT 75% + EABP 25% 3:1 13,0 13,6 9,5 12,0 12,5
100% 21,7 22,4 21,5 21,7 22,0 HVCO 50% + EABP 50% 1:1 11,1 11,0 10,2 10,4 11,6 HVCO 75% + EABP 25% 3:1 10,3 9,5 9,4 10,8 10,3 HVCO 25% + EABP 75% 1:3 16,1 1,0 18,3 17,5 17,7
Kadar (v/v) Zona hambat (mm) Pengulangan ke-
Blanko (DMSO+akuades 1:1) - - - - - EABP 10% 13,0 13,4 14,3 14,0 13,5 25% 16,0 16,1 17,0 1,0 16,9 50% 18,0 18,2 18,0 18,5 18,1 75% 19,3 19,6 19,0 19,5 19,3
HVCO 10% 7,0 8,5 7,3 8,0 8,3 25% 9,0 8,5 8,7 8,5 8,8 50% 10,1 9,2 10,0 9,4 9,0 75% 11,4 12,0 11,8 10,9 11,0 100% 13,8 14,0 13,0 13,9 13,5
VCOT 10% - - - - - 25% - - - - - 50% - - - 6,2 - 75% - - 6,6 6,3 - 100% 7,0 - 6,9 7,2 7,5
5 Blanko (DMSO) - - - - -
4
3
2
1
0,1 21,6 21,5 21,0 22,0 21,6 0,05 18,0 18,1 17,9 17,7 17,7 0,01 14,4 14,0 13,9 14,4 14,4 Keterangan: diameter pencadang kertas 6,0 mm; (-): tidak ada zona hambat;
Lampiran 26. Hasil analisis frekuensi dan ANAVA zona hambat terhadap
Bacillus cereus ATCC 14579VCOT+EABP 1:1 5 15.780 1.1967 .5352 14.294 17.266 14.3 17.6
6.0
6.6 VCOT 100% 5 6.920 .5630 .2518 6.221 7.619
6.0
7.5 Blanko DMSO+akuades 1:1 5 6.000 .0000 .0000 6.000 6.000
6.0
6.0 EABP 10% 5 13.640 .5128 .2293 13.003 14.277 13.0 14.3 EABP 25% 5 16.600 .5050 .2258 15.973 17.227 16.0 17.0 EABP 50% 5 18.160 .2074 .0927 17.903 18.417 18.0 18.5 EABP 75% 5 19.340 .2302 .1030 19.054 19.626 19.0 19.6 EABP 100% 5 21.860 .3507 .1568 21.425 22.295 21.5 22.4 HVCO+EABP 1:1 5 10.860 .5639 .2522 10.160 11.560 10.2 11.6 HVCO+EABP 3:1 5 10.060 .5941 .2657 9.322 10.798 9.4 10.8 HVCO+EABP 1:3 5 17.320 .8258 .3693 16.295 18.345 16.1 18.3
VCOT+EABP 3:1 5 12.120 1.5802 .7067 10.158 14.082 9.5 13.6
6.0
VCOT+EABP 1:3 5 18.700 .4359 .1949 18.159 19.241 18.0 19.1 Blanko akuades 5 6.000 .0000 .0000 6.000 6.000
6.0
6.0 Tetrasiklin HCl 5mg/ml 5 29.260 .3507 .1568 28.825 29.695 28.9 29.8 Tetrasiklin HCl 2.5mg/ml 5 27.440 .3286 .1470 27.032 27.848 26.9 27.7 Tetrasiklin HCl 1mg/ml 5 27.100 .2915 .1304 26.738 27.462 26.8 27.5 Tetrasiklin HCl 0.5mg/ml 5 24.720 .2049 .0917 24.466 24.974 24.4 24.9 Tetrasiklin HCl 0.1mg/ml 5 21.540 .3578 .1600 21.096 21.984 21.0 22.0 Tetrasiklin HCl 0.05mg/ml 5 17.880 .1789 .0800 17.658 18.102 17.7 18.1 Tetrasiklin HCl 0.01mg/ml 5 14.220 .2490 .1114 13.911 14.529 13.9 14.4 Total 155 14.092 7.0840 .5690 12.968 15.216 6.0 29.8
Analisis Variansi (ANAVA)
zona hambat (mm) Sum of Squares df Mean Square F Sig.
Between Groups 7695.047 30 256.502 960.216 .000
Within Groups 33.124 124 .267
6.2 VCOT 75% 5 6.180 .2683 .1200 5.847 6.513
6.0 VCOT 50% 5 6.040 .0894 .0400 5.929 6.151
zona hambat (mm) N Mean Std.
6.0
Deviati on Std.
Error 95% Confidence
Interval for Mean Min Max
Lower Bound
Upper Bound
Blanko DMSO 5 6.000 .0000 .0000 6.000 6.000
6.0 HVCO 10% 5 7.820 .6458 .2888 7.018 8.622
6.0
7.0
8.5 HVCO 25% 5 8.700 .2121 .0949 8.437 8.963
8.5
9.0 HVCO 50% 5 9.540 .4879 .2182 8.934 10.146 9.0 10.1 HVCO 75% 5 11.420 .4817 .2154 10.822 12.018 10.9 12.0 HVCO 100% 5 13.640 .4037 .1806 13.139 14.141 13.0 14.0
VCOT 10% 5 6.000 .0000 .0000 6.000 6.000
6.0
6.0 VCOT 25% 5 6.000 .0000 .0000 6.000 6.000
Total 7728.171 154
Lampiran 27. Hasil analisis Tukey HSD zona hambat terhadap Bacillus cereus ATCC 14579
5 19.340
EABP 25%
5 16.600 16.600
HVCO+EABP 1:3
5 17.320 17.320
Tetrasiklin HCl 0.05mg/ml
5 17.880 17.880
EABP 50%
5 18.160 18.160 18.160
VCOT+EABP 1:3
5 18.700 18.700
EABP 75%
Tetrasiklin HCl 0.1mg/ml
VCOT+EABP 1:1
5 21.540
EABP 100%
5 21.860
Tetrasiklin HCl 0.5mg/ml
5 24.720
Tetrasiklin HCl 1mg/ml
5 27.100
Tetrasiklin HCl 2.5mg/ml
5 27.440
Tetrasiklin HCl 5mg/ml
5 29.260
Sig. .555 .602 .650 .739 .999 .818 .050 .996 .781 .931 .739 .781 .103 1.000 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 5.000.
5 15.780
5 14.220
bahan uji N Subset for alpha = .05
12
1
2
3
4
5
6
7
8
9
10
11
13
Tetrasiklin HCl 0.01mg/ml
14
15
16
17 VCOT 10% 5 6.000
VCOT 25% 5 6.000 Blanko DMSO 5 6.000 Blanko akuades 5 6.000 Blanko DMSO+akuades 1:1 5 6.000
VCOT 50% 5 6.040
VCOT 75% 5 6.180
VCOT 100% 5 6.920 6.920 HVCO 10% 5 7.820 7.820 HVCO 25% 5 8.700 8.700 HVCO 50% 5 9.540 9.540 HVCO+EABP 3:1 5 10.060 10.060 HVCO+EABP 1:1 5 10.860 10.860 HVCO 75% 5 11.420
VCOT+EABP 3:1 5 12.120 HVCO 100%
5 13.640
EABP 10%
5 13.640
Universitas Sumatera Utara
Lampiran 28. Data pengukuran zona hambat terhadap Staphylococcus aureus
ATCC 25923 Bahan ujiVCOT 50% + EABP 50% 1:1 13,0 12,4 11,9 12,2 12,0
5 Blanko (akuades) - - - - - Tetrasiklin HCl 5 22,7 23,0 23,1 23,0 23,0 2,5 19,0 19,0 18,9 19,4 19,3 1 17,5 17,9 17,5 17,7 17,7 0,5 16,0 15,5 15,6 15,6 15,9
4
3
2
1
Zona hambat (mm) Pengulangan ke-
VCOT 25% + EABP 75% 1:3 13,8 13,5 13,0 14,6 13,5 Baku pembanding Kadar (mg/ml)
VCOT 75% + EABP 25% 3:1 10,0 12,0 11,0 11,2 11,5
100% 16,5 16,2 17,7 16,6 16,6 HVCO 50% + EABP 50% 1:1 10,3 11,9 12,6 11,6 12,0 HVCO 75% + EABP 25% 3:1 9,3 9,4 10,2 10,7 9,7 HVCO 25% + EABP 75% 1:3 12,0 11,0 10,8 13,0 12,5
Kadar (v/v) Zona hambat (mm) Pengulangan ke-
Blanko (DMSO+akuades 1:1) - - - - - EABP 10% 9,9 9,0 9,5 8,9 10,0 25% 10,8 11,0 11,0 12,5 11,6 50% 11,9 12,0 13,0 13,2 12,9 75% 13,2 15,4 15,0 14,7 13,9
HVCO 10% 8,7 9,4 8,5 8,6 9,0 25% 9,5 9,7 10,0 9,5 10,2 50% 10,7 9,9 10,5 10,7 11,1 75% 11,0 10,7 12,0 11,7 10,9 100% 14,0 13,6 14,0 13,6 14,5
VCOT 10% - - - - - 25% - - - - - 50% - - - - 6,6 75% 7,3 7,4 7,4 7,0 7,2 100% 9,0 10,7 9,3 8,9 9,0
5 Blanko (DMSO) - - - - -
4
3
2
1
0,1 14,1 14,8 14,0 14,3 14,4 0,05 10,5 10,4 10,0 9,9 10,1 0,01 7,0 8,1 7,3 7,0 7,2 Keterangan: diameter pencadang kertas 6,0 mm; (-): tidak ada zona hambat;
Lampiran 29. Hasil analisis frekuensi dan ANAVA zona hambat terhadap
Staphylococcus aureus ATCC 259236.0
7.4 VCOT 100% 5 9.380 .7530 .3367 8.445 10.315 8.9 10.7 Blanko DMSO+akuades 1:1 5 6.000 .0000 .0000 6.000 6.000
6.0
6.0 EABP 10% 5 9.460 .5030 .2249 8.835 10.085 8.9 10.0 EABP 25% 5 11.380 .6943 .3105 10.518 12.242 10.8 12.5 EABP 50% 5 12.600 .6042 .2702 11.850 13.350 11.9 13.2 EABP 75% 5 14.440 .8849 .3957 13.341 15.539 13.2 15.4 EABP 100% 5 16.720 .5718 .2557 16.010 17.430 16.2 17.7 HVCO+EABP 1:1 5 11.680 .8526 .3813 10.621 12.739 10.3 12.6 HVCO+EABP 3:1 5 9.860 .5857 .2619 9.133 10.587 9.3 10.7 HVCO+EABP 1:3 5 11.860 .9476 .4238 10.683 13.037 10.8 13.0
VCOT+EABP 1:1 5 12.300 .4359 .1949 11.759 12.841 11.9 13.0
VCOT+EABP 3:1 5 11.140 .7403 .3311 10.221 12.059 10.0 12.0
VCOT+EABP 1:3 5 13.680 .5891 .2634 12.949 14.411 13.0 14.6 Blanko akuades 5 6.000 .0000 .0000 6.000 6.000
6.0 Tetrasiklin HCl 5mg/ml 5 22.960 .1517 .0678 22.772 23.148 22.7 23.1 Tetrasiklin HCl 2.5mg/ml 5 19.120 .2168 .0970 18.851 19.389 18.9 19.4 Tetrasiklin HCl 1mg/ml 5 17.660 .1673 .0748 17.452 17.868 17.5 17.9 Tetrasiklin HCl 0.5mg/ml 5 15.720 .2168 .0970 15.451 15.989 15.5 16.0 Tetrasiklin HCl 0.1mg/ml 5 14.320 .3114 .1393 13.933 14.707 14.0 14.8 Tetrasiklin HCl 0.05mg/ml 5 10.180 .2588 .1158 9.859 10.501 9.9 10.5 Tetrasiklin HCl 0.01mg/ml 5 7.320 .4550 .2035 6.755 7.885
6.6 VCOT 75% 5 7.260 .1673 .0748 7.052 7.468
7.0
8.1 Total 155 11.276 4.2097 .3381 10.608 11.944 6.0 23.1
Analisis Variansi (ANAVA)
zona hambat (mm) Sum of Squares df Mean Square F Sig.
Between Groups 2699.866 30 89.996 381.181 .000
Within Groups 29.276 124 .236
7.0
6.0
Descriptives
Blanko DMSO 5 6.000 .0000 .0000 6.000 6.000
zona hambat (mm) N Mean Std.
Deviati on Std.
Error 95% Confidence
Interval for Mean Min Max
Lower Bound
Upper Bound
6.0
6.0 VCOT 50% 5 6.120 .2683 .1200 5.787 6.453
6.0 HVCO 10% 5 8.840 .3647 .1631 8.387 9.293
8.5
9.4 HVCO 25% 5 9.780 .3114 .1393 9.393 10.167 9.5 10.2 HVCO 50% 5 10.580 .4382 .1960 10.036 11.124 9.9 11.1 HVCO 75% 5 11.260 .5595 .2502 10.565 11.955 10.7 12.0 HVCO 100% 5 13.940 .3715 .1661 13.479 14.401 13.6 14.5
VCOT 10% 5 6.000 .0000 .0000 6.000 6.000
6.0
6.0 VCOT 25% 5 6.000 .0000 .0000 6.000 6.000
6.0
Total 2729.142 154
Lampiran 30. Hasil analisis Tukey HSD zona hambat terhadap Staphylococcus aureus ATCC 25923
EABP 75%
VCOT+EABP 1:1
5 12.300 12.300
EABP 50%
5 12.600 12.600
VCOT+EABP 1:3
5 13.680 13.680
HVCO 100%
5 13.940
Tetrasiklin HCl 0.1mg/ml
5 14.320
5 14.440
HVCO+EABP 1:3
Tetrasiklin HCl 0.5mg/ml
5 15.720
EABP 100%
5 16.720 16.720
Tetrasiklin HCl 1mg/ml
5 17.660
Tetrasiklin HCl 2.5mg/ml
5 19.120
Tetrasiklin HCl 5mg/ml
5 22.960
Sig. 1.000 .077 1.000 .215 .716 .093 .133 .112 .064 .418 .133 .803 .249 .371 1.000 1.000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 5.000.
5 11.860 11.860
5 11.680 11.680 11.680
bahan uji N Subset for alpha = .05
11
1
2
3
4
5
6
7
8
9
10
12
HVCO+EABP 1:1
13
14
15
16
17 VCOT 10% 5 6.000
VCOT 25% 5 6.000 Blanko DMSO 5 6.000 Blanko akuades 5 6.000 Blanko DMSO+akuades 1:1 5 6.000
VCOT 50% 5 6.120 6.120
VCOT 75% 5 7.260 7.260 Tetrasiklin HCl 0.01mg/ml 5 7.320 HVCO 10% 5 8.840
VCOT 100% 5 9.380 9.380 EABP 10% 5 9.460 9.460 9.460 HVCO 25% 5 9.780 9.780 9.780 HVCO+EABP 3:1 5 9.860 9.860 9.860 Tetrasiklin HCl 0.05mg/ml 5 10.180 10.180 10.180 HVCO 50% 5 10.580 10.580 10.580
VCOT+EABP 3:1 5 11.140 11.140 11.140 HVCO 75% 5 11.260 11.260 11.260 EABP 25%
5 11.380 11.380
Universitas Sumatera Utara
Lampiran 31. Gambar hasi uji uji antibakteri VCOT, HVCO, dan EABP terhadap Staphylococcus aureus ATCC 25923
Lampiran 32. Gambar hasi uji uji antibakteri baku pembanding Tetrasiklin HCl terhadap Staphylococcus aureus ATCC 25923
Lampiran 33. Data pengukuran zona hambat terhadap Escherichia coli ATCC 8939 Bahan uji
VCOT 50% + EABP 50% 1:1 10,4 11,7 9,7 10,7 TK
5 Blanko (akuades) - - - - - Tetrasiklin HCl 5 29,0 29,0 28,4 29,4 28,8 2,5 27,0 26,8 26,9 26,7 27,1 1 26,3 25,8 26,3 26,0 26,3 0,5 23,6 23,6 24,0 24,3 24,2
4
3
2
1
Zona hambat (mm) Pengulangan ke-
VCOT 25% + EABP 75% 1:3 11,6 11,0 12,9 11,0 TK Baku pembanding Kadar (mg/ml)
VCOT 75% + EABP 25% 3:1 7,0 8,0 8,4 7,4 TK
100% 17,0 18,5 18,6 18,0 17,7 HVCO 50% + EABP 50% 1:1 11,0 11,7 12,0 11,6 TK HVCO 75% + EABP 25% 3:1 10,2 10,5 9,9 8,9 TK HVCO 25% + EABP 75% 1:3 14,0 13,3 12,6 13,4 TK
Kadar (v/v) Zona hambat (mm) Pengulangan ke-
Blanko (DMSO+akuades 1:1) - - - - - EABP 10% 9,2 9,3 9,0 10,2 9,7 25% 10,3 11,4 12,4 11,9 12,0 50% 13,8 13,7 14,0 14,0 14,2 75% 14,5 15,2 15,9 15,0 14,7
HVCO 10% 8,7 8,8 7,7 8,5 8,5 25% 8,9 9,0 8,7 8,8 8,9 50% 9,8 10,3 9,5 9,7 10,0 75% 10,2 9,9 10,0 10,2 10,5 100% 11,2 10,6 11,0 11,0 11,1
VCOT 10% - - - - - 25% - - - - - 50% - - - - - 75% - - 6,3 - - 100% 7,5 8,0 7,3 8,2 7,5
5 Blanko (DMSO) - - - - -
4
3
2
1
0,1 20,0 19,7 20,2 20,1 20,1 0,05 18,8 18,7 19,0 19,3 19,3 0,01 14,6 14,5 14,5 15,0 14,5
Keterangan: diameter pencadang kertas 6,0 mm; (-): tidak ada zona hambat; TK:
terkontaminasi
Lampiran 34. Hasil analisis frekuensi dan ANAVA zona hambat terhadap
Escherichia coli ATCC 89397.0
6.3 VCOT 100% 5 7.700 .3808 .1703 7.227 8.173
7.3
8.2 Blanko DMSO+akuades 1:1 5 6.000 .0000 .0000 6.000 6.000
6.0
6.0 EABP 10% 5 9.480 .4764 .2131 8.888 10.072 9.0 10.2 EABP 25% 5 11.600 .8093 .3619 10.595 12.605 10.3 12.4 EABP 50% 5 13.940 .1949 .0872 13.698 14.182 13.7 14.2 EABP 75% 5 15.060 .5413 .2421 14.388 15.732 14.5 15.9 EABP 100% 5 18.260 .3912 .1749 17.774 18.746 17.7 18.6 HVCO+EABP 1:1 5 11.575 .4193 .2097 10.908 12.242 11.0 12.0 HVCO+EABP 3:1 5 9.875 .6946 .3473 8.770 10.980 8.9 10.5 HVCO+EABP 1:3 5 13.325 .5737 .2869 12.412 14.238 12.6 14.0
VCOT+EABP 1:1 5 10.625 .8302 .4151 9.304 11.946 9.7 11.7
VCOT+EABP 3:1 5 7.700 .6218 .3109 6.711 8.689
8.4 VCOT+EABP 1:3 5 11.625 .8958 .4479 10.200 13.050 11.0 12.9 Blanko akuades 5 6.000 .0000 .0000 6.000 6.000
6.0 VCOT 75% 5 6.060 .1342 .0600 5.893 6.227
6.0
6.0 Tetrasiklin HCl 5mg/ml 5 28.920 .3633 .1625 28.469 29.371 28.4 29.4 Tetrasiklin HCl 2.5mg/ml 5 26.900 .1581 .0707 26.704 27.096 26.7 27.1 Tetrasiklin HCl 1mg/ml 5 26.140 .2302 .1030 25.854 26.426 25.8 26.3 Tetrasiklin HCl 0.5mg/ml 5 23.940 .3286 .1470 23.532 24.348 23.6 24.3 Tetrasiklin HCl 0.1mg/ml 5 20.020 .1924 .0860 19.781 20.259 19.7 20.2 Tetrasiklin HCl 0.05mg/ml 5 19.020 .2775 .1241 18.675 19.365 18.7 19.3 Tetrasiklin HCl 0.01mg/ml 5 14.620 .2168 .0970 14.351 14.889 14.5 15.0
Total 149 12.676 6.7359 .5518 11.585 13.766 6.0 29.4
Analisis Variansi (ANAVA)
zona hambat (mm) Sum of Squares df Mean Square F Sig.
Between Groups 6696.848 30 223.228 1445.279 .000
Within Groups 18.226 118 .154
6.0
6.0
Descriptives
6.0
zona hambat (mm) N Mean Std.
Deviati on Std.
Error 95% Confidence
Interval for Mean Min Max
Lower Bound
Upper Bound
Blanko DMSO 5 6.000 .0000 .0000 6.000 6.000
6.0 HVCO 10% 5 8.440 .4336 .1939 7.902 8.978
6.0 VCOT 50% 5 6.000 .0000 .0000 6.000 6.000
7.7
8.8 HVCO 25% 5 8.860 .1140 .0510 8.718 9.002
8.7
9.0 HVCO 50% 5 9.860 .3050 .1364 9.481 10.239 9.5 10.3 HVCO 75% 5 10.160 .2302 .1030 9.874 10.446 9.9 10.5 HVCO 100% 5 10.980 .2280 .1020 10.697 11.263 10.6 11.2
VCOT 10% 5 6.000 .0000 .0000 6.000 6.000
6.0
6.0 VCOT 25% 5 6.000 .0000 .0000 6.000 6.000
6.0
Total 6715.073 148
Lampiran 35. Hasil analisis Tukey HSD zona hambat terhadap Escherichia coli ATCC 8939
Tetrasiklin HCl 0.05mg/ml
4 11.625
HVCO+EABP 1:3
4 13.325
EABP 50%
5 13.940 13.940
Tetrasiklin HCl 0.01mg/ml
5 14.620 14.620
EABP 75%
5 15.060
EABP 100%
5 18.260
5 19.020
5 11.600 11.600
Tetrasiklin HCl 0.1mg/ml
5 20.020
Tetrasiklin HCl 0.5mg/ml
5 23.940
Tetrasiklin HCl 1mg/ml
5 26.140
Tetrasiklin HCl 2.5mg/ml
5 26.900
Tetrasiklin HCl 5mg/ml
5 28.920
Sig. 1.000 .483 .999 .825 .665 .410 .268 .055 .763 .836 .665 .997 .424 1.000 1.000 .424 1.000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 4.769. b The group sizes are unequal. The harmonic mean of the group sizes is used. Type I error levels are not guaranteed
VCOT+EABP 1:3
EABP 25%
bahan uji N Subset for alpha = .05
12
1
2
3
4
5
6
7
8
9
10
11
13
4 11.575 11.575
14
15
16
17 VCOT 10% 5 6.000
VCOT 25% 5 6.000
VCOT 50% 5 6.000 Blanko DMSO 5 6.000 Blanko akuades 5 6.000
Blanko DMSO+akuades 1:1 5 6.000
VCOT 75% 5 6.060
VCOT+EABP 3:1 4 7.700
VCOT 100% 5 7.700 HVCO 10% 5 8.440 8.440 HVCO 25% 5 8.860 8.860 EABP 10% 5 9.480 9.480 HVCO 50% 5 9.860 9.860 HVCO+EABP 3:1 4 9.875 9.875 HVCO 75% 5 10.160 10.160 10.160
VCOT+EABP 1:1 4 10.625 10.625 10.625 HVCO 100% 5 10.980 10.980 10.980 HVCO+EABP 1:1
Universitas Sumatera Utara
Lampiran 36. Data pengukuran zona hambat terhadap Salmonella thypi ATCC 00786 Bahan uji
VCOT 50% + EABP 50% 1:1 9,3 10,2 9,3 10,6 TK
5 Blanko (akuades) - - - - - Tetrasiklin HCl 5 30,8 30,0 31,0 30,3 31,5 2,5 26,9 27,0 27,2 27,0 26,9 1 25,7 25,6 26,0 25,9 25,6 0,5 22,1 22,4 22,0 22,0 21,7
4
3
2
1
Zona hambat (mm) Pengulangan ke-
VCOT 25% + EABP 75% 1:3 11,0 10,0 11,5 11,4 TK Baku pembanding Kadar (mg/ml)
VCOT 75% + EABP 25% 3:1 8,2 7,5 7,0 9,3 TK
100% 20,5 20,0 19,9 19,9 19,7 HVCO 50% + EABP 50% 1:1 12,0 11,3 12,4 11,6 TK HVCO 75% + EABP 25% 3:1 11,2 10,0 9,6 9,0 TK HVCO 25% + EABP 75% 1:3 14,0 13,7 13,0 12,9 TK
Kadar (v/v) Zona hambat (mm) Pengulangan ke-
Blanko (DMSO+akuades 1:1) - - - - - EABP 10% 9,4 9,0 9,8 9,4 10,0 25% 10,4 10,5 10,0 10,7 10,0 50% 11,0 12,0 11,7 12,2 11,0 75% 14,6 15,0 15,6 15,7 15,3
HVCO 10% 9,4 9,0 9,8 9,4 10,0 25% 10,4 10,5 10,0 10,7 10,0 50% 11,0 12,0 11,7 12,2 11,0 75% 17,6 15,0 15,6 15,7 15,3 100% 20,5 20,0 19,0 19,9 19,7
VCOT 10% - - - - - 25% - - - - - 50% - - - - - 75% - - - - - 100% - 7,0 6,3 6,9 6,5
5 Blanko (DMSO) - - - - -
4
3
2
1
0,1 15,5 16,0 15,7 16,1 16,2 0,05 13,8 13,3 13,7 13,8 13,6 0,01 9,8 10,2 9,9 10,1 10,3
Keterangan: diameter pencadang kertas 6,0 mm; (-): tidak ada zona hambat; TK:
terkontaminasi
Lampiran 37. Hasil analisis frekuensi dan ANAVA zona hambat terhadap
Salmonella thypi ATCC 00786VCOT+EABP 3:1 5 8.000 .9967 .4983 6.414 9.586
6.0 VCOT 75% 5 6.000 .0000 .0000 6.000 6.000
6.0
6.0 VCOT 100% 5 6.540 .4159 .1860 6.024 7.056
6.0
7.0 Blanko DMSO+akuades 1:1 5 6.000 .0000 .0000 6.000 6.000
6.0
6.0 EABP 10% 5 9.520 .3899 .1744 9.036 10.004 9.0 10.0 EABP 25% 5 10.320 .3114 .1393 9.933 10.707 10.0 10.7 EABP 50% 5 11.580 .5586 .2498 10.886 12.274 11.0 12.2 EABP 75% 5 15.240 .4506 .2015 14.681 15.799 14.6 15.7 EABP 100% 5 19.820 .5450 .2437 19.143 20.497 19.0 20.5 HVCO+EABP 1:1 5 11.825 .4787 .2394 11.063 12.587 11.3 12.4 HVCO+EABP 3:1 5 9.950 .9292 .4646 8.472 11.428 9.0 11.2 HVCO+EABP 1:3 5 13.400 .5354 .2677 12.548 14.252 12.9 14.0
VCOT+EABP 1:1 5 9.850 .6557 .3279 8.807 10.893 9.3 10.6
7.0
6.0 VCOT 50% 5 6.000 .0000 .0000 6.000 6.000
9.3 VCOT+EABP 1:3 5 10.975 .6850 .3425 9.885 12.065 10.0 11.5 Blanko akuades 5 6.000 .0000 .0000 6.000 6.000
6.0
6.0 Tetrasiklin HCl 5mg/ml 5 30.720 .5891 .2634 29.989 31.451 30.0 31.5 Tetrasiklin HCl 2.5mg/ml 5 27.000 .1225 .0548 26.848 27.152 26.9 27.2 Tetrasiklin HCl 1mg/ml 5 25.760 .1817 .0812 25.534 25.986 25.6 26.0 Tetrasiklin HCl 0.5mg/ml 5 22.040 .2510 .1122 21.728 22.352 21.7 22.4 Tetrasiklin HCl 0.1mg/ml 5 15.900 .2915 .1304 15.538 16.262 15.5 16.2 Tetrasiklin HCl 0.05mg/ml 5 13.640 .2074 .0927 13.383 13.897 13.3 13.8 Tetrasiklin HCl 0.01mg/ml 5 10.060 .2074 .0927 9.803 10.317 9.8 10.3
Total 149 11.932 6.7112 .5498 10.845 13.018 6.0 31.5
Analisis Variansi (ANAVA)
zona hambat (mm) Sum of Squares df Mean Square F Sig.
Between Groups 6645.315 30 221.510 1268.415 .000
Within Groups 20.607 118 .175
6.0
6.0
Descriptives
6.0 HVCO 10% 5 7.400 .3937 .1761 6.911 7.889
zona hambat (mm) N Mean Std.
Deviati on Std.
Error 95% Confidence
Interval for Mean Min Max
Lower Bound
Upper Bound
Blanko DMSO 5 6.000 .0000 .0000 6.000 6.000
6.0
6.9
6.0 VCOT 25% 5 6.000 .0000 .0000 6.000 6.000
7.8 HVCO 25% 5 8.020 .4324 .1934 7.483 8.557
7.3
8.4 HVCO 50% 5 8.560 .4159 .1860 8.044 9.076
8.0
9.1 HVCO 75% 5 9.360 .3209 .1435 8.962 9.758
9.0
9.8 HVCO 100% 5 10.880 .4658 .2083 10.302 11.458 10.5 11.6
VCOT 10% 5 6.000 .0000 .0000 6.000 6.000
6.0
Total 6665.922 148
Lampiran 38. Hasil analisis Tukey HSD zona hambat terhadap Salmonella thypi ATCC 00786
5 15.900
5 11.580
HVCO+EABP 1:1
4 11.825
HVCO+EABP 1:3
4 13.400
Tetrasiklin HCl 0.05mg/ml
5 13.640
EABP 75%
5 15.240
Tetrasiklin HCl 0.1mg/ml
EABP 100%
4 10.975 10.975
5 19.820
Tetrasiklin HCl 0.5mg/ml
5 22.040
Tetrasiklin HCl 1mg/ml
5 25.760
Tetrasiklin HCl 2.5mg/ml
5 27.000
Tetrasiklin HCl 5mg/ml
5 30.720
Sig. .978 .294 .897 .965 .123 .123 .059 .062 .142 1.000 .823 1.000 1.000 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 4.769. b The group sizes are unequal. The harmonic mean of the group sizes is used. Type I error levels are not guaranteed.
EABP 50%
VCOT+EABP 1:3
bahan uji N Subset for alpha = .05
11
1
2
3
4
5
6
7
8
9
10
12
VCOT+EABP 1:1 4 9.850 9.850 HVCO+EABP 3:1 4 9.950 9.950 9.950 Tetrasiklin HCl 0.01mg/ml 5 10.060 10.060 10.060 EABP 25% 5 10.320 10.320 10.320 HVCO 100% 5 10.880 10.880 10.880
13
14
15
16
17 VCOT 10% 5 6.000
VCOT 25% 5 6.000
VCOT 50% 5 6.000
VCOT 75% 5 6.000 Blanko DMSO 5 6.000 Blanko akuades 5 6.000
Blanko DMSO+akuades 1:1 5 6.000
VCOT 100% 5 6.540 6.540 HVCO 10% 5 7.400 7.400
VCOT+EABP 3:1 4 8.000 8.000 HVCO 25% 5 8.020 8.020 HVCO 50% 5 8.560 8.560 HVCO 75% 5 9.360 9.360 EABP 10% 5 9.520 9.520
Universitas Sumatera Utara
Lampiran 39. Gambar hasi uji uji antibakteri VCOT, HVCO, dan EABP terhadap Salmonella thypi ATCC 00786
Lampiran 40. Gambar hasi uji uji antibakteri baku pembanding Tetrasiklin HCl terhadap Salmonella thypi ATCC 00786
Lampiran 41. Data pengukuran zona hambat terhadap Shigella dysenteriae
ATCC 13313 Bahan ujiVCOT 50% + EABP 50% 1:1 10,0 9,0 10,0 10,4 TK
5 Blanko (akuades) - - - - - Tetrasiklin HCl 5 28,5 28.0 28,4 28,0 28,5 2,5 26,6 26,7 26,7 26,0 26,5 1 24,0 24,5 24,9 24,0 24,0 0,5 22,5 23,0 23,1 22,7 22,8
4
3
2
1
Zona hambat (mm) Pengulangan ke-
VCOT 25% + EABP 75% 1:3 11,0 12,0 11,7 12,3 TK Baku pembanding Kadar (mg/ml)
VCOT 75% + EABP 25% 3:1 9,9 9,3 9,0 10,0 TK
100% 15,5 15,0 16,1 16,0 16,8 HVCO 50% + EABP 50% 1:1 13,0 12,9 13,4 12,5 TK HVCO 75% + EABP 25% 3:1 11,2 9,9 9,0 9,4 TK HVCO 25% + EABP 75% 1:3 13,5 14,9 13,9 14,0 TK
Kadar (v/v) Zona hambat (mm) Pengulangan ke-
Blanko (DMSO+akuades 1:1) - - - - - EABP 10% 8,8 8,0 8,5 9,0 8,7 25% 10,0 10,4 9,5 10,2 10,3 50% 10,9 11,0 12,0 11,2 11,5 75% 12,6 12,0 12,0 12,5 13,0
HVCO 10% 8,5 8,7 7,6 8,0 7,7 25% 9,4 9,0 9,2 8,9 9,4 50% 9,7 9,9 9,4 9,9 9,2 75% 10,0 10,2 9,8 9,7 10,0 100% 13,0 13,2 12,7 12,6 13,0
VCOT 10% - - - - - 25% - - - - - 50% - - - - - 75% - - - - - 100% - 6,3 7,2 7,0 6,7
5 Blanko (DMSO) - - - - -
4
3
2
1
0,1 19,0 19,9 18,9 19,5 19,4 0,05 18,3 18,3 18,0 17,8 18,4 0,01 14,0 13,5 13,6 14,2 13,6
Keterangan: diameter pencadang kertas 6,0 mm; (-): tidak ada zona hambat; TK:
terkontaminasi
Lampiran 42. Hasil analisis frekuensi dan ANAVA zona hambat terhadap
Shigella dysenteriae ATCC 13313VCOT+EABP 1:1 5 9.850 .5972 .2986 8.900 10.800 9.0 10.4
6.0
6.0 VCOT 100% 5 6.640 .4930 .2205 6.028 7.252
6.0
7.2 Blanko DMSO+akuades 1:1 5 6.000 .0000 .0000 6.000 6.000
6.0
6.0 EABP 10% 5 8.600 .3808 .1703 8.127 9.073
8.0
9.0 EABP 25% 5 10.080 .3564 .1594 9.638 10.522 9.5 10.4 EABP 50% 5 11.320 .4438 .1985 10.769 11.871 10.9 12.0 EABP 75% 5 12.420 .4266 .1908 11.890 12.950 12.0 13.0 EABP 100% 5 15.880 .6760 .3023 15.041 16.719 15.0 16.8 HVCO+EABP 1:1 5 12.950 .3697 .1848 12.362 13.538 12.5 13.4 HVCO+EABP 3:1 5 9.875 .9570 .4785 8.352 11.398 9.0 11.2 HVCO+EABP 1:3 5 14.100 .5598 .2799 13.209 14.991 13.6 14.9
VCOT+EABP 3:1 5 9.550 .4796 .2398 8.787 10.313 9.0 10.0
6.0
VCOT+EABP 1:3 5 11.750 .5568 .2784 10.864 12.636 11.0 12.3 Blanko akuades 5 6.000 .0000 .0000 6.000 6.000
6.0
6.0 Tetrasiklin HCl 5mg/ml 5 28.280 .2588 .1158 27.959 28.601 28.0 28.5 Tetrasiklin HCl 2.5mg/ml 5 26.500 .2915 .1304 26.138 26.862 26.0 26.7 Tetrasiklin HCl 1mg/ml 5 24.280 .4087 .1828 23.773 24.787 24.0 24.9 Tetrasiklin HCl 0.5mg/ml 5 22.820 .2387 .1068 22.524 23.116 22.5 23.1 Tetrasiklin HCl 0.1mg/ml 5 19.340 .4037 .1806 18.839 19.841 18.9 19.9 Tetrasiklin HCl 0.05mg/ml 5 18.160 .2510 .1122 17.848 18.472 17.8 18.4 Tetrasiklin HCl 0.01mg/ml 5 13.780 .3033 .1356 13.403 14.157 13.5 14.2
Total 149 12.225 6.3724 .5220 11.193 13.256 6.0 28.5
Analisis Variansi (ANAVA)
zona hambat (mm) Sum of Squares df Mean Square F Sig.
Between Groups 5993.023 30 199.767 1395.197 .000
Within Groups 16.896 118 .143
6.0 VCOT 75% 5 6.000 .0000 .0000 6.000 6.000
6.0 VCOT 50% 5 6.000 .0000 .0000 6.000 6.000
Descriptives
6.0 HVCO 10% 5 8.100 .4848 .2168 7.498 8.702
zona hambat (mm) N Mean Std.
Deviati on Std.
Error 95% Confidence
Interval for Mean Min Max
Lower Bound
Upper Bound
Blanko DMSO 5 6.000 .0000 .0000 6.000 6.000
6.0
7.6
6.0
8.7 HVCO 25% 5 9.180 .2280 .1020 8.897 9.463
8.9
9.4 HVCO 50% 5 9.620 .3114 .1393 9.233 10.007
9.2
9.9 HVCO 75% 5 9.940 .1949 .0872 9.698 10.182 9.7 10.2 HVCO 100% 5 12.900 .2449 .1095 12.596 13.204 12.6 13.2
VCOT 10% 5 6.000 .0000 .0000 6.000 6.000
6.0
6.0 VCOT 25% 5 6.000 .0000 .0000 6.000 6.000
Total 6009.918 148
Lampiran 43. Hasil analisis Tukey HSD zona hambat terhadap Shigella dysenteriae ATCC 13313
Tetrasiklin HCl 0.1mg/ml
VCOT+EABP 1:3 4 11.750 11.750 EABP 75% 5 12.420 12.420 HVCO 100% 5 12.900 12.900 HVCO+EABP 1:1 4 12.950 12.950 Tetrasiklin HCl 0.01mg/ml
5 13.780 13.780
HVCO+EABP 1:3
4 14.100
EABP 100%
5 15.880
Tetrasiklin HCl 0.05mg/ml
5 18.160
5 19.340
VCOT+EABP 3:1 4 9.550 HVCO 50% 5 9.620
Tetrasiklin HCl 0.5mg/ml
5 22.820
Tetrasiklin HCl 1mg/ml
5 24.280
Tetrasiklin HCl 2.5mg/ml
5 26.500
Tetrasiklin HCl 5mg/ml
5 28.280
Sig. .709 .970 .862 .087 .996 .617 .943 .109 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 Means for groups in homogeneous subsets are displayed. a Uses Harmonic Mean Sample Size = 4.769. b The group sizes are unequal. The harmonic mean of the group sizes is used. Type I error levels are not guaranteed.
VCOT+EABP 1:1 4 9.850 HVCO+EABP 3:1 4 9.875 HVCO 75% 5 9.940 EABP 25% 5 10.080 EABP 50% 5 11.320
VCOT 100% 5 6.640 HVCO 10% 5 8.100 EABP 10% 5 8.600 8.600 HVCO 25% 5 9.180 9.180
bahan uji N Subset for alpha = .05
9
1
2
3
4
5
6
7
8
10
VCOT 75% 5 6.000 Blanko DMSO 5 6.000 Blanko akuades 5 6.000 Blanko DMSO+akuades 1:1 5 6.000
11
12
13
14
15
16
17 VCOT 10% 5 6.000
VCOT 25% 5 6.000
VCOT 50% 5 6.000
Universitas Sumatera Utara
Lampiran 44. Gambar hasi uji uji antibakteri VCOT, HVCO, dan EABP terhadap Shigella dysenteriae ATCC 13313
Lampiran 45. Gambar hasi uji uji antibakteri baku pembanding Tetrasiklin HCl terhadap Shigella dysenteriae ATCC 13313
Lampiran 46. Data pengukuran zona hambat terhadap Vibrio cholera ATCC 39315 Bahan uji
VCOT 50% + EABP 50% 1:1 11,9 12,5 12,4 11,9 TK
5 Blanko (akuades) - - - - - Tetrasiklin HCl 5 27,7 27,3 28,0 27,7 28,2 2,5 24,4 24,0 23,8 24,1 23,5 1 22,5 22,5 22,1 21,9 22,4 0,5 21,6 21,0 21,5 20,9 21,6
4
3
2
1
Zona hambat (mm) Pengulangan ke-
VCOT 25% + EABP 75% 1:3 13,9 14,6 13,8 14,2 TK Baku pembanding Kadar (mg/ml)
VCOT 75% + EABP 25% 3:1 10,3 9,5 9,5 10,0 TK
100% 16,3 16,0 15,7 15,7 16,6 HVCO 50% + EABP 50% 1:1 14,0 14,0 14,5 14,8 TK HVCO 75% + EABP 25% 3:1 12,0 11,8 12,0 13,0 TK HVCO 25% + EABP 75% 1:3 16,2 1,4 17,0 17,3 TK
Kadar (v/v) Zona hambat (mm) Pengulangan ke-
Blanko (DMSO+akuades 1:1) - - - - - EABP 10% 9,4 8,3 8,6 8,8 9,0 25% 10,0 9,7 10,2 9,9 10,5 50% 11,7 11,3 11,0 12,0 11,5 75% 14,0 13,5 13,4 13,0 14,3
HVCO 10% 8,0 7,9 7,1 7,5 8,0 25% 8,5 9,0 8,5 8,3 8,6 50% 9,6 9,0 9,3 9,0 9,0 75% 9,9 10,1 9,7 10,7 10,2 100% 10.8 11,7 11,0 11,2 11,7
VCOT 10% - - - - - 25% - - - - - 50% - - - - - 75% - - 6,4 7,0 6,6 100% 7,3 7,3 7,9 7,7 7,7
5 Blanko (DMSO) - - - - -
4
3
2
1
0,1 18,5 19,0 18,7 18,7 18,5 0,05 15,5 15,5 16,2 15,7 15,5 0,01 12,8 12,0 12,3 13,0 12,3
Keterangan: diameter pencadang kertas 6,0 mm; (-): tidak ada zona hambat; TK:
terkontaminasiLampiran 47. Hasil analisis frekuensi dan ANAVA zona hambat terhadap Vibrio cholera ATCC 39315