Uji Toksisitas Subkronik Ekstrak Etanol Pecut Kuda (Stachytharpheta jamaicensis L.Vahl) Pada Mencit

(1)

(2)

(3)

(4)

Lampiran 4.

Bagan alur

penelitian uji toksisitas subkronik

Pengamatan selama 28 hari

P1: Kontrol,

diberi

larutan

CMC Na

P2:

Perlakuan,

diberi EEPK

dosis 200

mg/kg bb

P3:

Perlakuan,

diberi

EEPK dosis

400 mg/kg

bb

P4:

Perlakuan,

diberi

EEPK dosis

800 mg/kg

bb

Gejala toksik

Berat badan

Histopatologi

Hati

SGPT

Analisis data

Hewan uji

(mencit)

EEPK

Berat makanan

SGOT

Makroskopik

Hati

Kematian

(5)

Lampiran 5.

Perhitungan volume pemberian ekstrak etanol pecut kuda dosis

200, 400, dan 800 mg/kg bb

Dosis ekstrak etanol pecut kuda yang digunakan dalam penelitian ini adalah 200,

400, dan 800 mg/kg bb

1. EEPK dosis 200 mg/kg bb

Berat badan mencit = 25 g

Dosis pemberian =

� ��

= 5 mg

Konsentrasi ekstrak = 200 mg/10 ml

Volume pemberian =

��

�

= 0,25 ml

2. EEPK dosis 400 mg/kg bb

Berat badan mencit = 26,1g

Dosis pemberian =

, � ��

= 10,44 mg

Konsentrasi ekstrak = 200 mg/10 ml

Volume pemberian =

, ��

��

�

= 0,52 ml

3. EEPK dosis 800 mg/kg bb

Berat badan mencit = 27,7 g

Dosis pemberian =

, � ��

= 22,16 mg

Konsentrasi ekstrak = 400 mg/10 ml

Volume pemberian =

, ��

(6)

Lampiran 6.

Hasil uji pendahuluan

1. Hasil pengamatan kematian

Kel.

Jumlah

mencit

Hari ke-

∑

Persen

kematian

1-7

8 9-21

22 23-25

26 27-28

K1

8 -

0 0 %

K2

8 -

0 0%

K3

8 -

1J

-

1 12,5 %

K4

8 -

1J

-

1J

-

2 25%

Keterangan: K1 = kontrol; K2 = dosis 200 mg/kg bb; K3 = dosis 400 mg/kg bb;

K4 = dosis 800 mg/kg bb; J = Jantan

2. Hasil pengamatan gejala toksik

Kelompok

Hewan

Gejala Toksik

Hari ke-

1-4

5-14

15-21

22-28

Kontrol

Tremor

-

Diare

-

Salivasi

-

Lemas

-

Jalan mundur

-

Jalan dengan perut

-

Dosis 200

mg/kg bb

Tremor

-

Diare

-

Salivasi

-

Lemas

-

Jalan mundur

-

Jalan dengan perut

-

Dosis 400

mg/kg bb

Tremor

-

Diare

-

Salivasi

-

Lemas

-

+

Jalan mundur

-

Jalan dengan perut

-

Dosis 800

mg/kg bb

Tremor

-

Diare

-

Salivasi

-

Lemas

-

+

Jalan mundur

-

(7)

3. Hasil rata-rata berat badan

Kelompok

Rata-rata berat badan (g) ± SD pada hari ke-

1

7

14

21

28 K1

27,87 ±

4,03

29,72 ±

4,14

29,06 ±

4,56

28,87 ± 4,29

29,03 ± 4,16

K2

27,61 ±

3,38

27,65 ±

1,90

27,47 ±

1,99

26,92 ± 2,63

26,78 ± 2,69

K3

27,23 ±

4,15

28,23 ±

3,79

28,44 ±

3,05

27,95 ± 6,02

27,69 ± 2,66

K4

26,09 ±

3,78

25,59 ±

3,31

24,69 ±

3,25

25,55 ± 2,57

25,35 ± 2,51

Keterangan: K1 = kontrol; K2 = dosis 200 mg/kg bb; K3 = dosis 400 mg/kg bb;

K4 = dosis 800 mg/kg bb

4. Hasil rata-rata berat makanan

Kelompok

Rata-rata konsumsi makanan (g) ± SD pada minggu ke-

1

2

3

4 K1

25,39 ± 0,60

24,77 ± 0,60

25,13 ± 0,87

25,05 ± 0,65

K2

24,84 ± 1,09

24,44 ± 0,94

24,71 ± 0,82

24,92 ± 0,68

K3

25,15 ± 0,92

24,91 ± 0,78

24,99 ± 0,95

25,16 ± 0,37

K4

25,10 ± 0,86

24,91 ± 1,05

25,02 ± 0,48

25,16 ± 0,91

Keterangan: K1 = kontrol; K2 = dosis 200 mg/kg bb; K3 = dosis 400 mg/kg bb;

K4 = dosis 800 mg/kg bb

(8)

(9)

(10)

Lampiran 8.

Hasil rata-rata kadar SGPT dan SGOT

1. Hasil rata-rata pengukuran SGPT

Kelompok

Rata-rata kadar SGPT (IU/L) ± SD

Normal

38,00 ± 5,55

Dosis 200 mg/kg BB

46,00 ± 7,21

Dosis 400 mg/kg BB

78,71 ± 4,88*

Dosis 800 mg/kg BB

80,83 ± 5,77*

Keterangan: * = adanya perbedaan yang signifikan (p < 0,05)

2. Hasil rata-rata pengukuran SGOT

Kelompok

Rata-rata kadar SGOT (IU/L) ± SD

Normal

84,75 ± 14,22

Dosis 200 mg/kg BB

116,12 ± 68,02

Dosis 400 mg/kg BB

250,43 ± 36,63*

Dosis 800 mg/kg BB

312,83 ± 67,24*

(11)

Lampiran 9

. Gambar hewan percobaan

Pemberian sediaan uji secara oral

(12)

Lampiran 10.

Hasil analisis

spss

berat badan

Descriptive Statistics

N Mean Std. Deviation Minimum Maximum BBharike1 32 27.2044 3.72867 20.89 34.82 BBharike7 32 27.8019 3.56349 20.78 36.12 BBharike14 32 27.4150 3.60359 20.10 36.71 BBharike21 32 27.4150 3.60359 20.10 36.71 BBhariike28 32 27.3031 3.28575 21.32 35.67

One-Sample Kolmogorov-Smirnov Test

BBharike1 BBharike7 BBharike14 BBharike21 BBhariike28

N 32 32 32 32 32

Normal Parametersa Mean 27.2044 27.8019 27.4150 27.4150 27.3031 Std.

Deviation 3.72867 3.56349 3.60359 3.60359 3.28575 Most Extreme

Differences

Absolute .132 .133 .108 .108 .153 Positive .132 .133 .108 .108 .153 Negative -.077 -.120 -.086 -.086 -.113 Kolmogorov-Smirnov Z .747 .753 .609 .609 .865 Asymp. Sig. (2-tailed) .632 .622 .853 .853 .442 a. Test distribution is Normal.

(13)

Lampiran 10

. (Lanjutan)

Descriptives

N Mean

Std.

Deviation Std. Error

95% Confidence Interval for Mean

Min. Max. Lower Bound

Upper Bound BBharike

kontrol 8 27.8738 4.03861 1.42786 24.4974 31.2501 23.95 33.52 dosis 200

mg/kg bb 8 27.6175 3.38944 1.19835 24.7839 30.4511 23.56 34.82 dosis 400

mg/kg bb 8 27.2350 4.15955 1.47062 23.7575 30.7125 21.88 34.00 dosis 800

mg/kg bb 8 26.0913 3.78756 1.33910 22.9248 29.2577 20.89 31.34 Total 32 27.2044 3.72867 .65914 25.8600 28.5487 20.89 34.82 BBharike

kontrol 8 29.7262 4.14770 1.46643 26.2587 33.1938 25.73 36.12 dosis 200

mg/kg bb 8 27.6512 1.90562 .67374 26.0581 29.2444 25.71 30.41 dosis 400

mg/kg bb 8 28.2325 3.79278 1.34095 25.0617 31.4033 24.72 35.12 dosis 800

mg/kg bb 8 25.5975 3.31549 1.17220 22.8257 28.3693 20.78 30.27 Total 32 27.8019 3.56349 .62994 26.5171 29.0866 20.78 36.12 BBharike

kontrol 8 29.0600 4.56437 1.61375 25.2441 32.8759 23.74 36.71 dosis 200

mg/kg bb 8 27.4700 1.99600 .70569 25.8013 29.1387 24.67 30.41 dosis 400

mg/kg bb 8 28.4400 3.05076 1.07861 25.8895 30.9905 24.27 32.38 dosis 800

mg/kg bb 8 24.6900 3.25594 1.15115 21.9680 27.4120 20.10 28.38 Total 32 27.4150 3.60359 .63703 26.1158 28.7142 20.10 36.71 BBharike

kontrol 8 29.0600 4.56437 1.61375 25.2441 32.8759 23.74 36.71 dosis 200

(14)

dosis 400

mg/kg bb 8 28.4400 3.05076 1.07861 25.8895 30.9905 24.27 32.38 dosis 800

mg/kg bb 8 24.6900 3.25594 1.15115 21.9680 27.4120 20.10 28.38 Total 32 27.4150 3.60359 .63703 26.1158 28.7142 20.10 36.71 BBhariike

kontrol

8 28.7825 4.29808 1.51960 25.1892 32.3758 24.24 35.67

dosis 200 mg/kg bb

8 26.9225 2.63950 .93320 24.7158 29.1292 24.31 31.67

dosis 400 mg/kg bb

8 27.9525 3.01541 1.06611 25.4316 30.4734 25.11 32.10

dosis 800 mg/kg bb

8 25.5550 2.57627 .91085 23.4012 27.7088 21.32 29.33

Total

(15)

Lampiran 10.

(Lanjutan)

ANOVA

Sum of Squares df Mean Square F Sig. BBharike1 Between Groups 14.870 3 4.957 .334 .801

Within Groups 416.122 28 14.862

Total 430.992 31

BBharike7 Between Groups 70.165 3 23.388 2.024 .133 Within Groups 323.487 28 11.553

Total 393.652 31

BBharike14 Between Groups 89.482 3 29.827 2.668 .067 Within Groups 313.080 28 11.181

Total 402.563 31

BBharike21 Between Groups 46.488 3 15.496 1.506 .235 Within Groups 288.192 28 10.293

Total 334.680 31

BBhariike28 Between Groups 57.378 3 19.126 2.010 .135 Within Groups 266.423 28 9.515

(16)

Lampiran 11

. Hasil analisis

spss

berat makanan

Descriptive Statistics

N Mean Std. Deviation Minimum Maximum BM_Mingguke1 32 25.1250 .86797 23.00 26.37 BM_Mingguke2 32 24.7628 .84450 23.15 26.25 BM_Mingguke3 32 24.9650 .77959 23.00 26.25 BM_Mingguke4 32 25.0781 .65938 23.22 26.12

One-Sample Kolmogorov-Smirnov Test BM_Minggu ke1 BM_Minggu ke2 BM_Minggu ke3 BM_Minggu ke4

N 32 32 32 32

Normal Parametersa Mean 25.1250 24.7628 24.9650 25.0781 Std.

Deviation .86797 .84450 .77959 .65938 Most Extreme Differences Absolute .177 .167 .126 .152 Positive .109 .087 .100 .065 Negative -.177 -.167 -.126 -.152 Kolmogorov-Smirnov Z 1.001 .947 .711 .857 Asymp. Sig. (2-tailed) .269 .331 .693 .455 a. Test distribution is Normal.

Descriptives

N Mean

Std. Deviation

Std. Error

95% Confidence Interval for Mean

Min. Max. Lower Bound Upper Bound BM Minggu ke1

kontrol 8 25.3962 .60323 .21327 24.8919 25.9006 24.45 26.37 dosis 200 mg/kg bb 8 24.8462 1.09906 .38858 23.9274 25.7651 23.00 26.25 dosis 400 mg/kg bb 8 25.1525 .92113 .32567 24.3824 25.9226 23.15 26.12 dosis 800 mg/kg bb 8 25.1050 .86129 .30451 24.3849 25.8251 23.24 26.31

(17)

Total 32 25.1250 .86797 .15344 24.8121 25.4379 23.00 26.37 BM

Minggu ke2

kontrol 8 24.7788 .60690 .21457 24.2714 25.2861 24.12 25.43 dosis 200 mg/kg bb 8 24.4462 .94588 .33442 23.6555 25.2370 23.15 25.53 dosis 400 mg/kg bb 8 24.9125 .78689 .27821 24.2546 25.5704 23.51 25.55 dosis 800 mg/kg bb 8 24.9138 1.05344 .37245 24.0331 25.7944 23.43 26.25 Total 32 24.7628 .84450 .14929 24.4583 25.0673 23.15 26.25 BM

Minggu ke3

kontrol 8 25.1300 .87969 .31102 24.3946 25.8654 23.46 26.25 dosis 200 mg/kg bb 8 24.7112 .82009 .28995 24.0256 25.3969 23.00 25.35 dosis 400 mg/kg bb 8 24.9900 .95046 .33604 24.1954 25.7846 23.15 26.12 dosis 800 mg/kg bb 8 25.0288 .48132 .17017 24.6264 25.4311 24.13 25.64 Total 32 24.9650 .77959 .13781 24.6839 25.2461 23.00 26.25 BM

Minggu ke4

kontrol 8 25.0575 .65148 .23033 24.5129 25.6021 24.11 25.95 dosis 200 mg/kg bb 8 24.9288 .68782 .24318 24.3537 25.5038 23.98 25.67 dosis 400 mg/kg bb 8 25.1600 .37702 .13330 24.8448 25.4752 24.78 25.64 dosis 800 mg/kg bb 8 25.1662 .91830 .32467 24.3985 25.9340 23.22 26.12 Total 32 25.0781 .65938 .11656 24.8404 25.3159 23.22 26.12

ANOVA

Sum of Squares df Mean Square F Sig. BM_Mingguke1 Between Groups 1.219 3 .406 .514 .676

Within Groups 22.135 28 .791

Total 23.354 31

BM_Mingguke2 Between Groups 1.165 3 .388 .519 .672 Within Groups 20.944 28 .748

Total 22.109 31

BM_Mingguke3 Between Groups .770 3 .257 .398 .756 Within Groups 18.070 28 .645

Total 18.841 31

BM_Mingguke4 Between Groups .298 3 .099 .211 .888 Within Groups 13.181 28 .471

(18)

Lampiran 12

. Hasil analisis

spss

kadar SGPT

Descriptive Statistics

N Mean Std. Deviation Minimum Maximum

Kadar_SGPT 29 58.90 20.134 30 88

One-Sample Kolmogorov-Smirnov Test

Kadar_SGPT

N 29

Normal Parametersa Mean 58.90

Std. Deviation 20.134

Most Extreme Differences Absolute .203

Positive .203

Negative -.195

Kolmogorov-Smirnov Z 1.094

Asymp. Sig. (2-tailed) .182

Test distribution is Normal.

Descriptives Kadar_SGPT

N Mean Std. Deviation

Std. Error

95% Confidence Interval for Mean

Minimum Maximum Lower Bound Upper Bound

Kontrol 8 38.00 5.555 1.964 33.36 42.64 30 45 Dosis 200 mg/kg BB 8 46.00 7.211 2.550 39.97 52.03 36 60 Dosis 400 mg/kg BB 7 78.71 4.889 1.848 74.19 83.24 69 84 Dosis 800 mg/kg BB 6 80.83 5.776 2.358 74.77 86.90 72 88 Total 29 58.90 20.134 3.739 51.24 66.56 30 88

(19)

ANOVA Kadar_SGPT

Sum of Squares df Mean Square F Sig. Between Groups 10460.428 3 3486.809 97.915 .000 Within Groups 890.262 25 35.610

Total 11350.690 28

Multiple Comparisons Kadar_SGPT

Tukey HSD

(I) Perlakuan (J) Perlakuan

Mean Difference (I-J)

Std. Error Sig.

95% Confidence Interval Lower Bound Upper Bound Kontrol Dosis 200 mg/kg BB -8.000 2.984 .058 -16.21 .21 Dosis 400 mg/kg BB -40.714* 3.088 .000 -49.21 -32.22 Dosis 800 mg/kg BB -42.833* 3.223 .000 -51.70 -33.97 Dosis 200 mg/kg BB Kontrol 8.000 2.984 .058 -.21 16.21 Dosis 400 mg/kg BB -32.714* 3.088 .000 -41.21 -24.22 Dosis 800 mg/kg BB -34.833* 3.223 .000 -43.70 -25.97 Dosis 400 mg/kg BB Kontrol 40.714* 3.088 .000 32.22 49.21 Dosis 200 mg/kg BB 32.714* 3.088 .000 24.22 41.21 Dosis 800 mg/kg BB -2.119 3.320 .919 -11.25 7.01 Dosis 800 mg/kg BB Kontrol 42.833* 3.223 .000 33.97 51.70 Dosis 200 mg/kg BB 34.833* 3.223 .000 25.97 43.70 Dosis 400 mg/kg BB 2.119 3.320 .919 -7.01 11.25 *. The mean difference is significant at the 0.05 level.

(20)

Lampiran 12

. (Lanjutan)

Homogeneous Subsets

Kadar_SGPT Tukey HSD

Perlakuan N

Subset for alpha = 0.05

1 2

Kontrol 8 38.00

Dosis 200 mg/kg BB 8 46.00

Dosis 400 mg/kg BB 7 78.71

Dosis 800 mg/kg BB 6 80.83

Sig. .079 .907

(21)

Lampiran 13

. Hasil analisis

spss

kadar SGOT

Descriptive Statistics

N Mean Std. Deviation Minimum Maximum

Kadar_SGOT 29 180.59 105.267 63 429

One-Sample Kolmogorov-Smirnov Test

Kadar_SGOT

N 29

Normal Parametersa Mean 180.59

Std. Deviation 105.267

Most Extreme Differences Absolute .208

Positive .208

Negative -.153

Kolmogorov-Smirnov Z 1.122

Asymp. Sig. (2-tailed) .161

Test distribution is Normal.

Descriptives Kadar_SGOT

N Mean

Std. Deviation

Std. Error

95% Confidence Interval for Mean

Min. Max. Lower Bound Upper Bound

Kontrol 8 84.75 14.220 5.028 72.86 96.64 68 112 Dosis 200 mg/kg BB 8 116.12 68.026 24.051 59.25 173.00 63 273 Dosis 400 mg/kg BB 7 250.43 36.637 13.848 216.54 284.31 184 303 Dosis 800 mg/kg BB 6 312.83 67.247 27.453 242.26 383.40 248 429 Total 29 180.59 105.267 19.548 140.54 220.63 63 429

(22)

Lampiran 13

. (lanjutan)

ANOVA Kadar_SGOT

Sum of Squares df Mean Square F Sig. Between Groups 245800.112 3 81933.371 31.770 .000 Within Groups 64472.923 25 2578.917

Total 310273.034 28

Multiple Comparisons Kadar_SGOT

Tukey HSD

(I) Perlakuan (J) Perlakuan

Mean Difference (I-J)

Std. Error Sig.

95% Confidence Interval Lower Bound

Upper Bound Kontrol Dosis 200 mg/kg BB -31.375 25.392 .611 -101.22 38.47

Dosis 400 mg/kg BB -165.679* 26.283 .000 -237.97 -93.38 Dosis 800 mg/kg BB -228.083* 27.426 .000 -303.52 -152.64 Dosis 200 mg/kg BB Kontrol 31.375 25.392 .611 -38.47 101.22 Dosis 400 mg/kg BB -134.304* 26.283 .000 -206.60 -62.01 Dosis 800 mg/kg BB -196.708* 27.426 .000 -272.15 -121.27 Dosis 400 mg/kg BB Kontrol 165.679* 26.283 .000 93.38 237.97 Dosis 200 mg/kg BB 134.304* 26.283 .000 62.01 206.60 Dosis 800 mg/kg BB -62.405 28.253 .148 -140.12 15.31 Dosis 800 mg/kg BB Kontrol 228.083* 27.426 .000 152.64 303.52 Dosis 200 mg/kg BB 196.708* 27.426 .000 121.27 272.15 Dosis 400 mg/kg BB 62.405 28.253 .148 -15.31 140.12 *. The mean difference is significant at the 0.05 level.

(23)

Lampiran 13

. (lanjutan)

Homogeneous Subsets

Kadar_SGOT Tukey HSD

Perlakuan N

Subset for alpha = 0.05

1 2

Kontrol 8 84.75

Dosis 200 mg/kg BB 8 116.12

Dosis 400 mg/kg BB 7 250.43

Dosis 800 mg/kg BB 6 312.83

Sig. .652 .120

(24)

Lampiran 14

. Hasil analisis

spss

kadar kreatinin

Descriptive Statistics

N Mean Std. Deviation Minimum Maximum Kadar_Kreatinin 29 .3159 .18341 .10 .93

One-Sample Kolmogorov-Smirnov Test

Kadar_Kreatinin

N 29

Normal Parametersa Mean .3159

Std. Deviation .18341 Most Extreme Differences Absolute .181

Positive .181

Negative -.120

Kolmogorov-Smirnov Z .973

Asymp. Sig. (2-tailed) .300

a. Test distribution is Normal.

Descriptives Kadar_Kreatinin

N Mean Std. Deviation

Std. Error

95% Confidence Interval for Mean

Minimum Max. Lower Bound Upper Bound

Kontrol 8 .3025 .11841 .04187 .2035 .4015 .14 .48 Dosis 200 mg/kg bb 8 .2513 .12276 .04340 .1486 .3539 .10 .44 Dosis 400 mg/kg bb 7 .3457 .20816 .07868 .1532 .5382 .10 .62 Dosis 800 mg/kg bb 6 .3850 .28585 .11670 .0850 .6850 .13 .93 Total 29 .3159 .18341 .03406 .2461 .3856 .10 .93

ANOVA Kadar_Kreatinin

Sum of Squares df Mean Square F Sig.

Between Groups .070 3 .023 .666 .581

Within Groups .872 25 .035

(25)

Multiple Comparisons Kadar_Kreatinin

Tukey HSD

(I) Perlakuan (J) Perlakuan

Mean Difference (I-J)

Std. Error Sig.

95% Confidence Interval Lower Bound Upper Bound Kontrol Dosis 200 mg/kg bb .05125 .09339 .946 -.2056 .3081 Dosis 400 mg/kg bb -.04321 .09667 .970 -.3091 .2227 Dosis 800 mg/kg bb -.08250 .10087 .845 -.3600 .1950 Dosis 200 mg/kg bb Kontrol -.05125 .09339 .946 -.3081 .2056 Dosis 400 mg/kg bb -.09446 .09667 .763 -.3604 .1714 Dosis 800 mg/kg bb -.13375 .10087 .556 -.4112 .1437 Dosis 400 mg/kg bb Kontrol .04321 .09667 .970 -.2227 .3091 Dosis 200 mg/kg bb .09446 .09667 .763 -.1714 .3604 Dosis 800 mg/kg bb -.03929 .10391 .981 -.3251 .2465 Dosis 800 mg/kg bb Kontrol .08250 .10087 .845 -.1950 .3600 Dosis 200 mg/kg bb .13375 .10087 .556 -.1437 .4112 Dosis 400 mg/kg bb .03929 .10391 .981 -.2465 .3251

Kadar_Kreatinin Tukey HSD

Perlakuan N

Subset for alpha = 0.05

1 Dosis 200 mg/kg bb 8 .2513

Kontrol 8 .3025

Dosis 400 mg/kg bb 7 .3457 Dosis 800 mg/kg bb 6 .3850

Sig. .539

Means for groups in homogeneous subsets are displayed.

(26)

Lampiran 15

. Hasil analisis spss kadar ureum

Descriptive Statistics

N Mean Std. Deviation Minimum Maximum

Kadar_Ureum 29 45.62 4.238 40 57

One-Sample Kolmogorov-Smirnov Test

Kadar_Ureum

N 29

Normal Parametersa Mean 45.62

Std. Deviation 4.238 Most Extreme Differences Absolute .188

Positive .188

Negative -.092

Kolmogorov-Smirnov Z 1.015

Asymp. Sig. (2-tailed) .254

a. Test distribution is Normal.

Descriptives Kadar_Ureum

N Mean Std. Deviation

Std. Error

95% Confidence Interval for Mean

Minimum Maximum Lower Bound Upper Bound

Kontrol 8 41.88 1.808 .639 40.36 43.39 40 45 Dosis 200 mg/kg bb 8 45.88 3.563 1.260 42.90 48.85 41 52 Dosis 400 mg/kg bb 7 45.57 2.760 1.043 43.02 48.12 43 51 Dosis 800 mg/kg bb 6 50.33 4.457 1.820 45.66 55.01 45 57 Total 29 45.62 4.238 .787 44.01 47.23 40 57

ANOVA Kadar_Ureum

Sum of Squares df Mean Square F Sig. Between Groups 246.030 3 82.010 7.984 .001 Within Groups 256.798 25 10.272

(27)

ANOVA Kadar_Ureum

Sum of Squares df Mean Square F Sig. Between Groups 246.030 3 82.010 7.984 .001 Within Groups 256.798 25 10.272

Total 502.828 28

Multiple Comparisons Kadar_Ureum

Tukey HSD

(I) Perlakuan (J) Perlakuan

Mean Difference (I-J)

Std. Error Sig.

95% Confidence Interval Lower Bound Upper Bound Kontrol Dosis 200 mg/kg bb -4.000 1.602 .085 -8.41 .41 Dosis 400 mg/kg bb -3.696 1.659 .143 -8.26 .87 Dosis 800 mg/kg bb -8.458* 1.731 .000 -13.22 -3.70 Dosis 200 mg/kg bb Kontrol 4.000 1.602 .085 -.41 8.41 Dosis 400 mg/kg bb .304 1.659 .998 -4.26 4.87 Dosis 800 mg/kg bb -4.458 1.731 .072 -9.22 .30 Dosis 400 mg/kg bb Kontrol 3.696 1.659 .143 -.87 8.26 Dosis 200 mg/kg bb -.304 1.659 .998 -4.87 4.26 Dosis 800 mg/kg bb -4.762 1.783 .059 -9.67 .14 Dosis 800 mg/kg bb Kontrol 8.458* 1.731 .000 3.70 13.22 Dosis 200 mg/kg bb 4.458 1.731 .072 -.30 9.22 Dosis 400 mg/kg bb 4.762 1.783 .059 -.14 9.67 *. The mean difference is significant at the 0.05 level.

Kadar_Ureum Tukey HSD

Perlakuan N

Subset for alpha = 0.05

1 2

Kontrol 8 41.88

Dosis 400 mg/kg bb 7 45.57

Dosis 200 mg/kg bb 8 45.88 45.88 Dosis 800 mg/kg bb 6 50.33

Sig. .112 .065

(1)

Lampiran 13. (lanjutan)

ANOVA Kadar_SGOT

Sum of Squares df Mean Square F Sig. Between Groups 245800.112 3 81933.371 31.770 .000 Within Groups 64472.923 25 2578.917

Total 310273.034 28

Multiple Comparisons Kadar_SGOT

Tukey HSD

(I) Perlakuan (J) Perlakuan

Mean Difference (I-J)

Std. Error Sig.

95% Confidence Interval Lower Bound

Upper Bound Kontrol Dosis 200 mg/kg BB -31.375 25.392 .611 -101.22 38.47

(2)

Lampiran 13. (lanjutan)

Homogeneous Subsets

Kadar_SGOT Tukey HSD

Perlakuan N

Subset for alpha = 0.05

1 2

Kontrol 8 84.75

Dosis 200 mg/kg BB 8 116.12

Dosis 400 mg/kg BB 7 250.43

Dosis 800 mg/kg BB 6 312.83

Sig. .652 .120

(3)

Lampiran 14. Hasil analisis

spss

kadar kreatinin

Descriptive Statistics

N Mean Std. Deviation Minimum Maximum

Kadar_Kreatinin 29 .3159 .18341 .10 .93

One-Sample Kolmogorov-Smirnov Test

Kadar_Kreatinin

N 29

Normal Parametersa Mean .3159

Std. Deviation .18341 Most Extreme Differences Absolute .181

Positive .181

Negative -.120

Kolmogorov-Smirnov Z .973

Asymp. Sig. (2-tailed) .300

a. Test distribution is Normal.

Descriptives Kadar_Kreatinin

N Mean Std. Deviation

Std. Error

95% Confidence Interval for Mean

Minimum Max. Lower Bound Upper Bound

ANOVA Kadar_Kreatinin

Sum of Squares df Mean Square F Sig.

Between Groups .070 3 .023 .666 .581

Within Groups .872 25 .035

(4)

Multiple Comparisons Kadar_Kreatinin

Tukey HSD

(I) Perlakuan (J) Perlakuan

Mean Difference (I-J)

Std. Error Sig.

Kadar_Kreatinin Tukey HSD

Perlakuan N

Subset for alpha = 0.05

1 Dosis 200 mg/kg bb 8 .2513

Kontrol 8 .3025

Dosis 400 mg/kg bb 7 .3457 Dosis 800 mg/kg bb 6 .3850

Sig. .539

Means for groups in homogeneous subsets are displayed.

(5)

Lampiran 15. Hasil analisis spss kadar ureum

Descriptive Statistics

N Mean Std. Deviation Minimum Maximum

Kadar_Ureum 29 45.62 4.238 40 57

One-Sample Kolmogorov-Smirnov Test

Kadar_Ureum

N 29

Normal Parametersa Mean 45.62

Std. Deviation 4.238

Most Extreme Differences Absolute .188

Positive .188

Negative -.092

Kolmogorov-Smirnov Z 1.015

Asymp. Sig. (2-tailed) .254

a. Test distribution is Normal.

Descriptives Kadar_Ureum

N Mean Std. Deviation

Std. Error

95% Confidence Interval for Mean

Minimum Maximum Lower Bound Upper Bound

ANOVA Kadar_Ureum

Sum of Squares df Mean Square F Sig.

Between Groups 246.030 3 82.010 7.984 .001

(6)

ANOVA Kadar_Ureum

Sum of Squares df Mean Square F Sig.

Between Groups 246.030 3 82.010 7.984 .001

Within Groups 256.798 25 10.272

Total 502.828 28

Multiple Comparisons Kadar_Ureum

Tukey HSD

(I) Perlakuan (J) Perlakuan

Mean Difference (I-J)

Std. Error Sig.

Kadar_Ureum Tukey HSD

Perlakuan N

Subset for alpha = 0.05

1 2

Kontrol 8 41.88

Dosis 400 mg/kg bb 7 45.57

Dosis 200 mg/kg bb 8 45.88 45.88

Dosis 800 mg/kg bb 6 50.33

Sig. .112 .065