Uji Antidiabetes Ekstrak Etil Asetat Daun Kelapa Sawit (Elaeis Guineensis, Jacq.) Terhadap Mencit Jantan
UJI ANTIDIABETES EKSTRAK ETIL ASETAT DAUN KELAPA SAWIT
(Elaeis guineensis, Jacq.) TERHADAP MENCIT JANTAN
ABSTRAK
Penyakit diabetes melitus merupakan penyakit yang menjadi permasalahan
medis pada saat ini.Meningkatnya penderita diabetes melitus dari tahun ketahun
memerlukan usaha untuk mengatasinya.Kandungan metabolit sekunder daun
kelapa sawit dapat digunakan sebagai antidiabetes. Penelitian ini bertujuan untuk
menguji penurunan kadar glukosa darah (KGD) dari ekstrak etil asetat daun
kelapa sawit (Elaeis guineensis,Jacq.) terhadap mencit jantan.
Serbuk daun kelapa sawit diekstraksi secara maserasi menggunakan
pelarut etil asetat, kemudian di rotary evaporator dengan bantuan
vakum.Selanjutnya dilakukan skrining terhadap simplisia dan ekstrak etil asetat
daun kelapa sawit (EEADKS), diuji aktivitas antidiabetes dengan metode toleransi
glukosa dan yang diinduksi aloksan terhadap mencit jantan, terdiri dari 6
kelompok. Kelompok 1: kontrol negatif (Na-CMC 0,5%), kelompok 2-5 diberi
EEADKS dengan variasi dosis 50, 75, 100, 125 mg/kg bb dan kelompok 6
kelompok pembanding (glibenklamid untuk metode toleransi glukosa dan
metformin untuk metode induksi aloksan). Pada metode toleransi glukosa 30
menit setelah perlakuan(variasi dosis EEADKS),diberi larutan glukosa 3 g/kg bb,
kemudian KGD diukur pada menit ke- 30, 60, 90 dan 120. Pada metode induksi
aloksan mencit diabet diberi perlakuan dandiukur KGD pada hari ke- 3, 5, 7, 9,
11, 13 dan 15. Data yang diperoleh dianalisis menggunakan ANAVA, dilanjutkan
dengan uji Post-Hoc Tukey.
Hasil skrining fitokimia terhadap serbuk simplisia dan ekstrak etil asetat
daun kelapa sawit diperoleh adanya senyawa golongan alkaloid, saponin,
flavonoid, tanin dan glikosida.Hasil penurunan KGD baik metode uji toleransi
glukosa dan induksi aloksan memberikan hasil yang signifikan (p0,05).
Kata kunci:Antidiabetes, daun kelapa sawit, ekstrak etil asetat, kadar
glukosadarah, mencit jantan.
Universitas Sumatera Utara
ANTIDIABETIC TEST OF ETHYL ACETATE PALM LEAVES
(Elaeis guineensis, Jacq.) EXTRACT ON MALE MICE
ABSTRACT
Diabetes mellitus is a disease that becomes a medical problem today.
Increased diabetes mellitus from year to year requires efforts to overcome it. The
secondary metabolite of oil palm leaves can be used as antidiabetes. The study
aims to test the decrease of blood glucose level (BGL) from ethyl acetate extract
of oil palm leaves (Elaeis guineensis, Jacq.) on male mice.
The extraction of palm leaves powder was prepared by maceration with
ethyl acetate, then the macerate was concentrated by vacum rotary evaporator.
Screening of simplicia and ethyl acetate palm leaf extract (EAPLE) was examined
the antidiabetic activity with glucose tolerance method and alloxan-induced on
male mice, consisted of 6 groups. Group 1 was negative control (Na-CMC 0.5%),
group 2-5 were given EAPLE with dose variation of 50, 75, 100, 125 mg /kg bw
and group of 6 as a comparison group (glibenclamide for glucose tolerance
method and metformin for induction alloxan method). In 30 minutes after
treatment of glucose tolerance method (variation of EAPLE dose), dose 3 g/kgbw
glucose solution was given, and BGL was measured at 30, 60, 90 and 120
minutes. In the alloxan induction method diabetics were treated and measured by
BGL on day 3, 5, 7, 9, 11, 13 and 15. The result were analyzed using ANOVA,
and continued with Post-Hoc Tukey test.
The result of phytochemical screening on the powder of simplex and the
extract of ethyl acetate of palm oil leaves was obtained alkaloid group, saponin,
flavonoid, tannin and glycoside. The decrease of BGL both of glucose tolerance
and alloxan induction test method gave a significant result (p 0.05).
Keywords:Antidiabetic, palm leaf , ethyl acetate extract, blood glucose level,
male mice
Universitas Sumatera Utara
(Elaeis guineensis, Jacq.) TERHADAP MENCIT JANTAN
ABSTRAK
Penyakit diabetes melitus merupakan penyakit yang menjadi permasalahan
medis pada saat ini.Meningkatnya penderita diabetes melitus dari tahun ketahun
memerlukan usaha untuk mengatasinya.Kandungan metabolit sekunder daun
kelapa sawit dapat digunakan sebagai antidiabetes. Penelitian ini bertujuan untuk
menguji penurunan kadar glukosa darah (KGD) dari ekstrak etil asetat daun
kelapa sawit (Elaeis guineensis,Jacq.) terhadap mencit jantan.
Serbuk daun kelapa sawit diekstraksi secara maserasi menggunakan
pelarut etil asetat, kemudian di rotary evaporator dengan bantuan
vakum.Selanjutnya dilakukan skrining terhadap simplisia dan ekstrak etil asetat
daun kelapa sawit (EEADKS), diuji aktivitas antidiabetes dengan metode toleransi
glukosa dan yang diinduksi aloksan terhadap mencit jantan, terdiri dari 6
kelompok. Kelompok 1: kontrol negatif (Na-CMC 0,5%), kelompok 2-5 diberi
EEADKS dengan variasi dosis 50, 75, 100, 125 mg/kg bb dan kelompok 6
kelompok pembanding (glibenklamid untuk metode toleransi glukosa dan
metformin untuk metode induksi aloksan). Pada metode toleransi glukosa 30
menit setelah perlakuan(variasi dosis EEADKS),diberi larutan glukosa 3 g/kg bb,
kemudian KGD diukur pada menit ke- 30, 60, 90 dan 120. Pada metode induksi
aloksan mencit diabet diberi perlakuan dandiukur KGD pada hari ke- 3, 5, 7, 9,
11, 13 dan 15. Data yang diperoleh dianalisis menggunakan ANAVA, dilanjutkan
dengan uji Post-Hoc Tukey.
Hasil skrining fitokimia terhadap serbuk simplisia dan ekstrak etil asetat
daun kelapa sawit diperoleh adanya senyawa golongan alkaloid, saponin,
flavonoid, tanin dan glikosida.Hasil penurunan KGD baik metode uji toleransi
glukosa dan induksi aloksan memberikan hasil yang signifikan (p0,05).
Kata kunci:Antidiabetes, daun kelapa sawit, ekstrak etil asetat, kadar
glukosadarah, mencit jantan.
Universitas Sumatera Utara
ANTIDIABETIC TEST OF ETHYL ACETATE PALM LEAVES
(Elaeis guineensis, Jacq.) EXTRACT ON MALE MICE
ABSTRACT
Diabetes mellitus is a disease that becomes a medical problem today.
Increased diabetes mellitus from year to year requires efforts to overcome it. The
secondary metabolite of oil palm leaves can be used as antidiabetes. The study
aims to test the decrease of blood glucose level (BGL) from ethyl acetate extract
of oil palm leaves (Elaeis guineensis, Jacq.) on male mice.
The extraction of palm leaves powder was prepared by maceration with
ethyl acetate, then the macerate was concentrated by vacum rotary evaporator.
Screening of simplicia and ethyl acetate palm leaf extract (EAPLE) was examined
the antidiabetic activity with glucose tolerance method and alloxan-induced on
male mice, consisted of 6 groups. Group 1 was negative control (Na-CMC 0.5%),
group 2-5 were given EAPLE with dose variation of 50, 75, 100, 125 mg /kg bw
and group of 6 as a comparison group (glibenclamide for glucose tolerance
method and metformin for induction alloxan method). In 30 minutes after
treatment of glucose tolerance method (variation of EAPLE dose), dose 3 g/kgbw
glucose solution was given, and BGL was measured at 30, 60, 90 and 120
minutes. In the alloxan induction method diabetics were treated and measured by
BGL on day 3, 5, 7, 9, 11, 13 and 15. The result were analyzed using ANOVA,
and continued with Post-Hoc Tukey test.
The result of phytochemical screening on the powder of simplex and the
extract of ethyl acetate of palm oil leaves was obtained alkaloid group, saponin,
flavonoid, tannin and glycoside. The decrease of BGL both of glucose tolerance
and alloxan induction test method gave a significant result (p 0.05).
Keywords:Antidiabetic, palm leaf , ethyl acetate extract, blood glucose level,
male mice
Universitas Sumatera Utara