Appendix Uji Toksisitas Akut Ekstrak Etanol Herba Selada Air (Nasturtium officinale R. Br.) Pada Organ Hati Mencit

Lampiran 1. Hasil Identifikasi Tumbuhan

57

Lampiran 2. Rekomendasi Persetujuan Etik Penelitian Kesehatan

58

Lampiran 3. Tumbuhan Selada Air

59

Lampiran 4. Alat – Alat yang Digunakan

Spuit dan Oral sonde

Neraca listrik

60

Neraca hewan

Alat bedah

61

Mikroskop

62

Lampiran 5. Hewan Penelitian dan Pengamatan Gejala Toksik

Hewan Penelitian

Uji Panggung

Uji Defekasi

63

Uji Urinasi

Uji Katalepsi

64

Lampiran 6. Pengukuran Organ Hati Mencit Jantan dan Mencit Betina

Pengukuran Organ Hati Mencit Jantan

Pengukuran Organ Hati Mencit Betina

65

Lampiran 7. Hasil Analisis Data Secara Statistik
Berat Badan Mencit Minggu I
Descriptive Statistics
Dependent Variable:Rata-Rata Berat Minggu I
Dosis Perlakuan

Jenis

Kelamin

Kontrol

Jantan

2.433002E
1

Betina

2.418666E
1
2.425834E
1

Total
50 mg/Kg BB

Jantan

Betina
Total

500 mg/Kg BB

Jantan
Betina
Total

1000 mg/Kg BB

Jantan
Betina
Total

2000 mg/Kg BB

Jantan
Betina
Total

4000 mg/Kg BB

Jantan
Betina
Total

Total

Mean

2.431336E
1
2.428334E
1
2.429835E
1
2.512666E
1
2.518332E

1
2.515499E
1
2.512666E
1
2.505000E
1
2.508833E
1
2.478664E
1
2.440000E
1
2.459332E
1
2.552666E
1
2.474802E
1
2.513734E

1

Jantan

2.486833E
1

Betina

2.464189E
1
2.475511E
1

Total

66

Std. Deviation

N

.7316575

5

.8451785

5

.7490719

10

.5530785

5

1.1247140

5

.8357141

10

1.0670805

5

1.3285861

5

1.1364288

10

.4747086

5

1.4304720

5

1.0056001

10

1.6316191

5

1.2285406

5

1.3767795

10

1.4240464

5

1.3315490

5

1.3629798

10

1.0737915

30

1.1834851

30

1.1261562

60

Multiple Comparisons
Rata-Rata Berat Minggu I
LSD

Mean
Difference
(I-J)

95% Confidence
Interval

(I) Dosis
Perlakuan

(J) Dosis
Perlakuan

Kontrol

50 mg/Kg BB

-.040010

.5158868

.939

-1.077270

.997250

500 mg/Kg BB

-.896650

.5158868

.089

-1.933910

.140610

1000 mg/Kg BB

-.829990

.5158868

.114

-1.867250

.207270

2000 mg/Kg BB

-.334980

.5158868

.519

-1.372240

.702280

4000 mg/Kg BB

-.879000

.5158868

.095

-1.916260

.158260

.040010

.5158868

.939

500 mg/Kg BB

-.856640

.5158868

.103

-1.893900

.180620

1000 mg/Kg BB

-.789980

.5158868

.132

-1.827240

.247280

2000 mg/Kg BB

-.294970

.5158868

.570

-1.332230

.742290

4000 mg/Kg BB

-.838990

.5158868

.110

-1.876250

.198270

Kontrol

.896650

.5158868

.089

-.140610 1.933910

50 mg/Kg BB

.856640

.5158868

.103

-.180620 1.893900

1000 mg/Kg BB

.066660

.5158868

.898

-.970600 1.103920

2000 mg/Kg BB

.561670

.5158868

.282

-.475590 1.598930

4000 mg/Kg BB

.017650

.5158868

.973

-1.019610 1.054910

50 mg/Kg BB

500 mg/Kg BB

Kontrol

1000 mg/Kg BB Kontrol

Std. Error

Sig.

Lower
Bound

Upper
Bound

-.997250 1.077270

.829990

.5158868

.114

-.207270 1.867250

50 mg/Kg BB

.789980

.5158868

.132

-.247280 1.827240

500 mg/Kg BB

-.066660

.5158868

.898

2000 mg/Kg BB

.495010

.5158868

.342

4000 mg/Kg BB

-.049010

.5158868

.925

2000 mg/Kg BB Kontrol

-1.103920

.970600

-.542250 1.532270
-1.086270

.988250

.334980

.5158868

.519

-.702280 1.372240

50 mg/Kg BB

.294970

.5158868

.570

-.742290 1.332230

500 mg/Kg BB

-.561670

.5158868

.282

-1.598930

.475590

1000 mg/Kg BB

-.495010

.5158868

.342

-1.532270

.542250

4000 mg/Kg BB

-.544020

.5158868

.297

-1.581280

.493240

.879000

.5158868

.095

50 mg/Kg BB

.838990

.5158868

.110

-.198270 1.876250

500 mg/Kg BB

-.017650

.5158868

.973

-1.054910 1.019610

1000 mg/Kg BB

.049010

.5158868

.925

-.988250 1.086270

2000 mg/Kg BB

.544020

.5158868

.297

-.493240 1.581280

4000 mg/Kg BB Kontrol

Based on observed means.
The error term is Mean Square(Error) = 1,331.

67

-.158260 1.916260

Tests of Between-Subjects Effects
Dependent Variable:Rata-Rata Berat Minggu I
Source
Corrected Model
Intercept
Dosis
Sex

Type III Sum of
Squares

Df

Mean Square

F

Sig.

a

11

.996

.748

.688

36768.933

1

36768.933

2.763E4

.000

8.986

5

1.797

1.351

.260

10.952

.769

1

.769

.578

.451

1.197

5

.239

.180

.969

Error

63.873

48

1.331

Total

36843.759

60

74.825

59

Dosis * Sex

Corrected Total

a. R Squared = ,146 (Adjusted R Squared = -,049)

68

Berat Badan Mencit Minggu II
Descriptive Statistics
Dependent Variable:Rata-Rata Berat Minggu II
Dosis Perlakuan

Jenis
Kelamin

Kontrol

Jantan

2.741334E
1

Betina

2.778332E
1
2.759833E
1

Total
50 mg/Kg BB

Jantan
Betina
Total

500 mg/Kg BB

Jantan
Betina
Total

1000 mg/Kg BB

Jantan
Betina
Total

2000 mg/Kg BB

Jantan
Betina
Total

4000 mg/Kg BB

Jantan
Betina
Total

Total

Mean

2.806668E
1
2.676002E
1
2.741335E
1
2.840334E
1
2.714334E
1
2.777334E
1
2.901332E
1
2.770334E
1
2.835833E
1
2.825998E
1
2.724332E
1
2.775165E
1
2.897000E
1
2.727666E
1
2.812333E
1

Jantan

2.835444E
1

Betina

2.731833E
1
2.783639E
1

Total

69

Std. Deviation

N

1.0831545

5

.8531847

5

.9396692

10

.9478600

5

1.2959044

5

1.2727760

10

1.0435086

5

1.8977049

5

1.5891918

10

1.3501204

5

1.0727818

5

1.3410138

10

1.5486864

5

2.1838992

5

1.8635502

10

1.3636741

5

.6010143

5

1.3354900

10

1.2584866

30

1.3484473

30

1.3946856

60

Multiple Comparisons
Rata-Rata Berat Minggu II
LSD

Mean
Difference
(I-J)

95% Confidence
Interval

(I) Dosis
Perlakuan

(J) Dosis
Perlakuan

Kontrol

50 mg/Kg BB

.184980

.5990479

.759

-1.019487

1.389447

500 mg/Kg BB

-.175010

.5990479

.771

-1.379477

1.029457

1000 mg/Kg BB

-.760000

.5990479

.211

-1.964467

.444467

2000 mg/Kg BB

-.153320

.5990479

.799

-1.357787

1.051147

4000 mg/Kg BB

-.525000

.5990479

.385

-1.729467

.679467

Kontrol

-.184980

.5990479

.759

-1.389447

1.019487

500 mg/Kg BB

-.359990

.5990479

.551

-1.564457

.844477

1000 mg/Kg BB

-.944980

.5990479

.121

-2.149447

.259487

2000 mg/Kg BB

-.338300

.5990479

.575

-1.542767

.866167

4000 mg/Kg BB

-.709980

.5990479

.242

-1.914447

.494487

Kontrol

.175010

.5990479

.771

-1.029457

1.379477

50 mg/Kg BB

.359990

.5990479

.551

-.844477

1.564457

1000 mg/Kg BB

-.584990

.5990479

.334

-1.789457

.619477

2000 mg/Kg BB

.021690

.5990479

.971

-1.182777

1.226157

4000 mg/Kg BB

-.349990

.5990479

.562

-1.554457

.854477

50 mg/Kg BB

500 mg/Kg BB

1000 mg/Kg BB Kontrol

Std. Error

Sig.

Lower
Bound

Upper
Bound

.760000

.5990479

.211

-.444467

1.964467

50 mg/Kg BB

.944980

.5990479

.121

-.259487

2.149447

500 mg/Kg BB

.584990

.5990479

.334

-.619477

1.789457

2000 mg/Kg BB

.606680

.5990479

.316

-.597787

1.811147

4000 mg/Kg BB

.235000

.5990479

.697

-.969467

1.439467

2000 mg/Kg BB Kontrol

.153320

.5990479

.799

-1.051147

1.357787

50 mg/Kg BB

.338300

.5990479

.575

-.866167

1.542767

500 mg/Kg BB

-.021690

.5990479

.971

-1.226157

1.182777

1000 mg/Kg BB

-.606680

.5990479

.316

-1.811147

.597787

4000 mg/Kg BB

-.371680

.5990479

.538

-1.576147

.832787

.525000

.5990479

.385

-.679467

1.729467

50 mg/Kg BB

.709980

.5990479

.242

-.494487

1.914447

500 mg/Kg BB

.349990

.5990479

.562

-.854477

1.554457

1000 mg/Kg BB

-.235000

.5990479

.697

-1.439467

.969467

2000 mg/Kg BB

.371680

.5990479

.538

-.832787

1.576147

4000 mg/Kg BB Kontrol

Based on observed means.
The error term is Mean Square(Error) = 1,794.

70

Tests of Between-Subjects Effects
Dependent Variable:Rata-Rata Berat Minggu II
Source

Type III Sum of
Squares

Df

Mean Square

F

Sig.

a

11

2.603

1.451

.182

46491.871

1

46491.871

2.591E4

.000

6.015

5

1.203

.671

.648

16.103

1

16.103

8.974

.004

6.519

5

1.304

.727

.607

Error

86.126

48

1.794

Total

46606.635

60

114.764

59

Corrected Model
Intercept
Dosis
Sex
Dosis * Sex

Corrected Total

28.638

a. R Squared = ,250 (Adjusted R Squared = ,078)

71

Berat Organ hati

Descriptive Statistics
Dependent Variable:Berat Organ Relatif Hati
Dosis Perlakuan

Jenis
Kelamin

Kontrol

Jantan

5.366652E
0

Betina

5.149684E
0
5.258168E
0

Total
50 mg/Kg BB

Jantan
Betina
Total

500 mg/Kg BB

Jantan
Betina
Total

1000 mg/Kg BB

Jantan
Betina
Total

2000 mg/Kg BB

Jantan
Betina
Total

4000 mg/Kg BB

Jantan
Betina
Total

Total

Mean

5.439530E
0
5.247826E
0
5.343678E
0
5.338790E
0
5.608970E
0
5.473880E
0
5.511650E
0
5.057424E
0
5.284537E
0
5.299578E
0
5.393030E
0
5.346304E
0
5.536872E
0
5.224800E
0
5.380836E
0

Jantan

5.415512E
0

Betina

5.280289E
0
5.347901E
0

Total

72

Std. Deviation

N

.3951808

5

.2328523

5

.3264693

10

.2417794

5

.3054974

5

.2786914

10

.3501781

5

.5775840

5

.4722764

10

.1229841

5

.2529150

5

.3040774

10

.1982225

5

.1270257

5

.1645005

10

.3530887

5

.2957396

5

.3483304

10

.2814166

30

.3500181

30

.3221698

60

Multiple Comparisons
Berat Organ Relatif Hati
LSD

Mean
Difference
(I-J)

95% Confidence
Interval

(I) Dosis
Perlakuan

(J) Dosis
Perlakuan

Kontrol

50 mg/Kg BB

-.085510

.1393469

.542

-.365686

.194666

500 mg/Kg BB

-.215712

.1393469

.128

-.495888

.064464

1000 mg/Kg BB

-.026369

.1393469

.851

-.306545

.253807

2000 mg/Kg BB

-.088136

.1393469

.530

-.368312

.192040

4000 mg/Kg BB

-.122668

.1393469

.383

-.402844

.157508

.085510

.1393469

.542

-.194666

.365686

-.130202

.1393469

.355

-.410378

.149974

1000 mg/Kg BB

.059141

.1393469

.673

-.221035

.339317

2000 mg/Kg BB

-.002626

.1393469

.985

-.282802

.277550

4000 mg/Kg BB

-.037158

.1393469

.791

-.317334

.243018

Kontrol

.215712

.1393469

.128

-.064464

.495888

50 mg/Kg BB

.130202

.1393469

.355

-.149974

.410378

1000 mg/Kg BB

.189343

.1393469

.181

-.090833

.469519

2000 mg/Kg BB

.127576

.1393469

.364

-.152600

.407752

4000 mg/Kg BB

.093044

.1393469

.508

-.187132

.373220

50 mg/Kg BB

Kontrol
500 mg/Kg BB

500 mg/Kg BB

1000 mg/Kg BB Kontrol

Std. Error

Sig.

Lower
Bound

Upper
Bound

.026369

.1393469

.851

-.253807

.306545

50 mg/Kg BB

-.059141

.1393469

.673

-.339317

.221035

500 mg/Kg BB

-.189343

.1393469

.181

-.469519

.090833

2000 mg/Kg BB

-.061767

.1393469

.660

-.341943

.218409

4000 mg/Kg BB

-.096299

.1393469

.493

-.376475

.183877

2000 mg/Kg BB Kontrol

.088136

.1393469

.530

-.192040

.368312

50 mg/Kg BB

.002626

.1393469

.985

-.277550

.282802

500 mg/Kg BB

-.127576

.1393469

.364

-.407752

.152600

1000 mg/Kg BB

.061767

.1393469

.660

-.218409

.341943

4000 mg/Kg BB

-.034532

.1393469

.805

-.314708

.245644

.122668

.1393469

.383

-.157508

.402844

50 mg/Kg BB

.037158

.1393469

.791

-.243018

.317334

500 mg/Kg BB

-.093044

.1393469

.508

-.373220

.187132

1000 mg/Kg BB

.096299

.1393469

.493

-.183877

.376475

2000 mg/Kg BB

.034532

.1393469

.805

-.245644

.314708

4000 mg/Kg BB Kontrol

Based on observed means.
The error term is Mean Square(Error) = ,097.

73

Tests of Between-Subjects Effects
Dependent Variable:Berat Organ Relatif Hati
Source

Type III Sum of
Squares

Df

Mean Square

F

Sig.

a

11

.133

1.370

.218

1716.002

1

1716.002

1.767E4

.000

Dosis

.290

5

.058

.598

.701

Sex

.274

1

.274

2.825

.099

Dosis * Sex

.899

5

.180

1.852

.121

Error

4.660

48

.097

Total

1722.126

60

6.124

59

Corrected Model
Intercept

Corrected Total

1.464

a. R Squared = ,239 (Adjusted R Squared = ,065)

74