KARYA TULIS ILMIAH 22. pdf
KARYA TULIS ILMIAH
PEMERIKSAAN KOLIFORM TOTAL TERHADAP
AIR MINUM ISI ULANG MERK DN, FT, NC
DIWILAYAH KECAMATAN
BUDURAN - SIDOARJO
FAHMI ARDIANTI PURNAWIRANITA, S.Farm., Apt
AKADEMI FARMASI MITRA SEHAT MANDIRI
SIDOARJO
2012
ABSTRACT
FAHMI ARDIANTI PURNAWIRANITA, S.Farm., Apt,2012 RESEARCH OF
TOTAL KOLIFORM IN GALLONS DRINKING WATER BRANDED A, B,
AND C IN SIDOARJO REGENCY, AKADEMI FARMASI MITRA SEHAT
MANDIRI.
Gallons drinking water has been investigated branded DN, FT and NC taken from
three locations in Sidoarjo regency. To find the number of total coliform in 100
ml refill gallons of water, we used the method of multiple tube or Most Probable
Number (MPN). From the result obtained the three sample of drinking water
doesnot meet the requirements of the Minister of Health Decree No.
907/MENKES SK/VII/2002 reveiewed in terms of coliform bacteria.
Keywords: Total Koliform , Drinking Water, JPT
ii
DAFTAR ISI
HALAMAN SAMPUL DEPAN ..............................................................................
i
HALAMAN SAMPUL DALAM ............................................................................
ii
HALAMAN LEMBAR PENGESAHAN ...............................................................
iii
HALAMAN LEMBAR PENETAPAN TIM PENGUJI ..........................................
iv
KATA PENGANTAR .............................................................................................
v
ABSTRAK ...............................................................................................................
vii
DAFTAR ISI ............................................................................................................
ix
DAFTAR TABEL ....................................................................................................
xii
DAFTAR GAMBAR .................................................................................................
xiii
DAFTAR LAMPIRAN ............................................................................................
xvi
I.
PENDAHULUAN .........................................................................................
1
1.1.
Latar Belakang .....................................................................................
1
1.2.
Rumusan Masalah ................................................................................
2
1.3.
Tujuan Penelitian ...............................................................................
2
1.4.
Manfaat ...............................................................................................
2
TINJAUAN PUSTAKA .................................................................................
4
2.1.
AIR ......................................................................................................
4
2.1.1. Macam-macam Air ....................................................................
5
2.1.2. Jenis-jenis Air Minum ..............................................................
5
Bakteri ..................................................................................................
7
2.2.1. Morfologi Bakteri ....................................................................
7
2.2.2. Susunan Sel Bakteri .................................................................
9
2.3.
Koliform ..............................................................................................
9
2.4.
Escherichia coli ..................................................................................
11
II.
2.2.
iii
2.4.1. Penyakit yang ditimbulkan .....................................................
11
2.4.2. Manfaat Escherichia coli ........................................................
11
Jumlah Perkiraan Terdekat .................................................................
12
METODE PENELITIAN ...............................................................................
14
3.1.
Jenis Penelitian ....................................................................................
14
3.2.
Populasi dan sampel ............................................................................
14
3.2.1. Populasi .....................................................................................
14
3.2.2. Sampel ....................................................................................
14
Variabel Penelitian ...............................................................................
14
3.3.1. Variabel Bebas ..........................................................................
14
3.3.2. Varaibel Terikat ......................................................................
14
3.3.2. Varaibel kontrol ......................................................................
15
Tempat dan Waktu Penelitian ..............................................................
15
3.4.1. Tempat Penelitian .....................................................................
15
3.4.2. Waktu Penelitian .....................................................................
15
Alat dan Bahan ...................................................................................
15
3.5.1. Alat ..........................................................................................
15
3.5.2. Bahan ......................................................................................
15
3.6.
Sterilisasi Alat ......................................................................................
16
3.7.
Pembuatan Media ...............................................................................
16
3.7.1. Media Lactose Broth ...............................................................
16
3.7.2. Media Brilliant Green Lactose Bill Broth ..............................
16
Pengambilan Sampel ..........................................................................
16
3.8.1. Cara Pengerjaan ......................................................................
16
Penentuan Jumlah Perkiraan Terdekat (JPT) ......................................
17
3.9.1. Tes Perkiraan ..........................................................................
17
3.9.2. Tes Penegasan .........................................................................
17
2.5.
III.
3.3.
3.4.
3.5.
3.8.
3.9.
3.9.3. Skema kerja Penentuan Jumlah Perkiraan Terdekat
Sampel Air Minum Galon Isi Ulang ........................................
IV.
METODE PENELITIAN
4.1.
18
..............................................................................
19
Hasil Pemeriksaan Jumlah Perkiraan Terdekat Bakteri Koliform .........
19
4.1.1. Hasil Pemeriksaan JPT Pada Sampel Air Mimun Isi ULang
iv
Merek DN ......................................................................................
19
4.1.2. Hasil Pemeriksaan JPT Pada Sampel Air Mimun Isi ULang
Merek NC .......................................................................................
20
4.1.2. Hasil Pemeriksaan JPT Pada Sampel Air Mimun Isi ULang
Merek FT ........................................................................................
21
PEMBAHASAN .............................................................................................
22
VI. KESIMPULAN ..................................................................................................
24
VII. SARAN..............................................................................................................
25
RINGKASAN .......................................................................................................
26
DAFTAR PUSTAKA ...........................................................................................
28
LAMPIRAN .........................................................................................................
29
V.
v
PEMERIKSAAN KOLIFORM TOTAL TERHADAP
AIR MINUM ISI ULANG MERK DN, FT, NC
DIWILAYAH KECAMATAN
BUDURAN - SIDOARJO
FAHMI ARDIANTI PURNAWIRANITA, S.Farm., Apt
AKADEMI FARMASI MITRA SEHAT MANDIRI
SIDOARJO
2012
ABSTRACT
FAHMI ARDIANTI PURNAWIRANITA, S.Farm., Apt,2012 RESEARCH OF
TOTAL KOLIFORM IN GALLONS DRINKING WATER BRANDED A, B,
AND C IN SIDOARJO REGENCY, AKADEMI FARMASI MITRA SEHAT
MANDIRI.
Gallons drinking water has been investigated branded DN, FT and NC taken from
three locations in Sidoarjo regency. To find the number of total coliform in 100
ml refill gallons of water, we used the method of multiple tube or Most Probable
Number (MPN). From the result obtained the three sample of drinking water
doesnot meet the requirements of the Minister of Health Decree No.
907/MENKES SK/VII/2002 reveiewed in terms of coliform bacteria.
Keywords: Total Koliform , Drinking Water, JPT
ii
DAFTAR ISI
HALAMAN SAMPUL DEPAN ..............................................................................
i
HALAMAN SAMPUL DALAM ............................................................................
ii
HALAMAN LEMBAR PENGESAHAN ...............................................................
iii
HALAMAN LEMBAR PENETAPAN TIM PENGUJI ..........................................
iv
KATA PENGANTAR .............................................................................................
v
ABSTRAK ...............................................................................................................
vii
DAFTAR ISI ............................................................................................................
ix
DAFTAR TABEL ....................................................................................................
xii
DAFTAR GAMBAR .................................................................................................
xiii
DAFTAR LAMPIRAN ............................................................................................
xvi
I.
PENDAHULUAN .........................................................................................
1
1.1.
Latar Belakang .....................................................................................
1
1.2.
Rumusan Masalah ................................................................................
2
1.3.
Tujuan Penelitian ...............................................................................
2
1.4.
Manfaat ...............................................................................................
2
TINJAUAN PUSTAKA .................................................................................
4
2.1.
AIR ......................................................................................................
4
2.1.1. Macam-macam Air ....................................................................
5
2.1.2. Jenis-jenis Air Minum ..............................................................
5
Bakteri ..................................................................................................
7
2.2.1. Morfologi Bakteri ....................................................................
7
2.2.2. Susunan Sel Bakteri .................................................................
9
2.3.
Koliform ..............................................................................................
9
2.4.
Escherichia coli ..................................................................................
11
II.
2.2.
iii
2.4.1. Penyakit yang ditimbulkan .....................................................
11
2.4.2. Manfaat Escherichia coli ........................................................
11
Jumlah Perkiraan Terdekat .................................................................
12
METODE PENELITIAN ...............................................................................
14
3.1.
Jenis Penelitian ....................................................................................
14
3.2.
Populasi dan sampel ............................................................................
14
3.2.1. Populasi .....................................................................................
14
3.2.2. Sampel ....................................................................................
14
Variabel Penelitian ...............................................................................
14
3.3.1. Variabel Bebas ..........................................................................
14
3.3.2. Varaibel Terikat ......................................................................
14
3.3.2. Varaibel kontrol ......................................................................
15
Tempat dan Waktu Penelitian ..............................................................
15
3.4.1. Tempat Penelitian .....................................................................
15
3.4.2. Waktu Penelitian .....................................................................
15
Alat dan Bahan ...................................................................................
15
3.5.1. Alat ..........................................................................................
15
3.5.2. Bahan ......................................................................................
15
3.6.
Sterilisasi Alat ......................................................................................
16
3.7.
Pembuatan Media ...............................................................................
16
3.7.1. Media Lactose Broth ...............................................................
16
3.7.2. Media Brilliant Green Lactose Bill Broth ..............................
16
Pengambilan Sampel ..........................................................................
16
3.8.1. Cara Pengerjaan ......................................................................
16
Penentuan Jumlah Perkiraan Terdekat (JPT) ......................................
17
3.9.1. Tes Perkiraan ..........................................................................
17
3.9.2. Tes Penegasan .........................................................................
17
2.5.
III.
3.3.
3.4.
3.5.
3.8.
3.9.
3.9.3. Skema kerja Penentuan Jumlah Perkiraan Terdekat
Sampel Air Minum Galon Isi Ulang ........................................
IV.
METODE PENELITIAN
4.1.
18
..............................................................................
19
Hasil Pemeriksaan Jumlah Perkiraan Terdekat Bakteri Koliform .........
19
4.1.1. Hasil Pemeriksaan JPT Pada Sampel Air Mimun Isi ULang
iv
Merek DN ......................................................................................
19
4.1.2. Hasil Pemeriksaan JPT Pada Sampel Air Mimun Isi ULang
Merek NC .......................................................................................
20
4.1.2. Hasil Pemeriksaan JPT Pada Sampel Air Mimun Isi ULang
Merek FT ........................................................................................
21
PEMBAHASAN .............................................................................................
22
VI. KESIMPULAN ..................................................................................................
24
VII. SARAN..............................................................................................................
25
RINGKASAN .......................................................................................................
26
DAFTAR PUSTAKA ...........................................................................................
28
LAMPIRAN .........................................................................................................
29
V.
v