Formulasi Dan Uji Efek Anti-Aging Krim Ekstrak Serat Mesokarp Kelapa Sawit

  Lampiran 1. Bagan proses ekstraksi serat mesokarp kelapa sawit 20 kg serat

• dimasukkan ke dalam wadah gelap
• diekstraksi dengan pelarut n -heksan sebanyak 80 L dengan perbandingan>ditutup dengan penutup wadah dan diselotip kemudian didiamkan selama 2×24 jam
• disaring dengan kain kemudian dimasukkan ke dalam jerigen
• ampas serat kemudian dimaserasi kembali selama 2×24 jam sebanyak 2 kali
• digabung semua hasil maserat Ekstrak n-heksan serat mesokarp kelapa sawit
• Diuapkan dengan rotary evaporator pada suhu ±40°C
• disentrifuge untuk mengendapkan asam lemak bebas Ekstrak serat mesokarp kelapa sawit kental

  Keterangan: Ekstrak serat mesokarp kelapa sawit kental tersebut kemudia diuji

  kadar air, asam lemak bebas (ALB), nilai bilangan DOBI, kadar karoten dan diuji aktivitas antioksidan menggunakan metode DPPH (1,1 diphenyl-2-picryl-hidrazyl).

  Lampiran 2. Bagan pembuatan sediaan krim Fase minyak Fase air

• ditimbang 12 gram asam stearat - 5 gram sorbitol
• ditimbang 0,5 gram setil alkohol - 3 gram propilen glikol
• dimasukkan kedalam cawan - 1 gram trietanolamin

  penguap - 0,2 gram metil paraben

• dilebur di atas penangas air pada - dilarutkan dalam air panas bersuhu 70°C yang telah dit>direndam lumpang dan alu dengan air panas dengan suhu 70ºC selama 10 menit
• dikeringkan lumpang dan dimasukkan fase minyak ke dalam lumpang, kemudian ditambahkan fase air Fase minyak + fase air
• digerus konstan di dalam lumpang sampai hampir terbentuk massa krim
• ditambahkan ekstrak serat mesokarp kelapa sawit dengan variasi konsentrasi pada masing-masing formula krim dan 3 tetes oleum citri
• dihomogenkan sampai terbentuk krim Krim ekstrak serat mesokarp kelapa sawit

  Lampiran 3. Gambar proses penghasilan serat mesokarp kelapa sawit

A B C

D E F

G

  

Keterangan: A: Buah kelapa sawit yang akan direbus, B: Alat perebus, C: Alat

pengempa buah kelapa sawit, D: Proses penyaringan minyak mentah, E: Tanki minyak mentah, F: Serat mesokarp kelapa sawit dalam

mesin, G: serat mesokarp kelapa sawit setelah pengeringan

  Lampiran 4. Gambar alat-alat penelitian H

  I J

K L M

N

Keterangan: H: Wadah maserasi, I: Rotary evaporator vacuum (BUCHI), J:

Jerigen maserat, K: Sentrifuge, L: Timbangan analitik (kg), M:

  Timbangan analitik (SATORUS), N: Spektrofotometer UV-Visible Shimadzu 1700

  Lampiran 4. (Lanjutan)

A D C

D E F

G

  

Keterangan: A: Mikropipet (Socorex), B: Labu ukur 10 ml, C: pH meter (Hanna

Instrumen), D: Moisturizer checker (Aramo), E: Skin analyzer (Aramo), F: Viskometer (Brookfield), G: Spidel 64

  

Lampiran 5. Gambar ekstrak serat mesokarp kelapa sawit

  

Lampiran 6. Gambar sediaan krim blanko dan krim ekstrak serat mesokarp

kelapa sawit setelah dibuat sampai 90 hari dalam suhu kamar

• Setelah dibuat di dalam lumpang Dasar krim (blanko) Krim 0,5%

  Krim 1% Krim 2%

  Krim 3% Krim 5%

  Lampiran 6. (Lanjutan)

• Setelah dibuat dalam wadah

F1 F2 F3 F4 F5 F6

• Setelah 7 hari

F1 F2 F3 F4 F5 F6

• Setelah 14 hari

F1 F2 F3 F4 F5 F6

  Lampiran 6. (Lanjutan)

• Setelah 21 hari

F1 F2 F3 F4 F5 F6

• Setelah 28 hari

F1 F2 F3 F4 F5 F6

• Setelah 90 hari

F1 F2 F3 F4 F5 F6

  

Keterangan: F: Formula, F1: blanko (tanpa ekstrak) dan krim ekstrak serat

mesokarp kelapa sawit F2: 0,5%, F3: 1%, F4: 2%, F5: 3%, F6: 5%

  Lampiran 7. Gambar hasil uji tipe emulsi dan homogenitas sediaan krim

  a. tipe emulsi

Keterangan: Hasil penentuan tipe emulsi krim A= blanko, krim ekstrak serat

mesokarp kelapa sawit B= 0,5%, C= 1%, D= 2%, E= 3% dan F=

  5%

  b. homogenitas

Keterangan: Hasil penentuan tipe emulsi krim A= blanko, krim ekstrak serat

mesokarp kelapa sawit B= 0,5%, C= 1%, D= 2%, E= 3% dan F=

  5%

  

Lampiran 8. Salah satu contoh hasil uji efektivitas anti-aging pada kulit mata

bagian lateral sukarelawan

a.. Hasil pengukuran kadar air (Moisture)

• Kondisi awal
• Pemulihan minggu pertama (Minggu 1)
• Pemulihan minggu kedua (Minggu 2)
• Pemulihan minggu ketiga (Minggu 3)
• Pemulihan minggu keempat (Minggu 4)

  Lampiran 8. (Lanjutan)

b. Hasil pengukuran kehalusan dan besar pori (Evenness/pore)

• Kondisi awal (Minggu 0)
• Pemulihan minggu pertama (Minggu 1)

  Lampiran 8. (Lanjutan)

• Pemulihan minggu kedua (Minggu 2)
• Pemulihan minggu ketiga (Minggu 3)

  Lampiran 8. (Lanjutan)

• - Pemulihan minggu keempat (minggu 4)

  Lampiran 8. (Lanjutan)

c. Hasil pengukuran noda (Spot)

• Kondisi awal (Minggu 0)

• - Pemulihan minggu pertama (minggu 1)

  Lampiran 8. (Lanjutan)

• Pemulihan minggu kedua (minggu 2)
• Pemulihan minggu ketiga (minggu 3)

  Lampiran 8. (Lanjutan)

• - Pemulihan minggu keempat (minggu 4)

  Lampiran 8. (Lanjutan)

d. Hasil pengukuran keriput (Wrinkle) dan kedalaman keriput (Wrinkle’s

  Depth )

• Kondisi awal (Minggu 0)
• Pemulihan minggu pertama (Minggu 1)

  Lampiran 8. (Lanjutan)

• Pemulihan minggu kedua (Minggu 2)
• Pemulihan minggu ketiga (Minggu 3)

  Lampiran 8. (Lanjutan)

• - Pemulihan minggu keempat (Minggu 4)

  

Lampiran 9. Perhitungan hasil uji karaterisasi serat dan ekstrak serat mesokarp

kelapa sawit a. perhitungan kadar air serat

  

Kode BS BW Oven 1 Oven 2 %

SP Pabatu 10,4311 g 214,3254 g 215,1483 g 214,9604 g 4,31

Keterangan: BS = Berat serat, BW = Berat wadah

  W 1 − W2 % Kadar air= 100

  × W 1

  Keterangan: W1 = Berat serat sebelum dioven W2 = Berat serat setelah dioven 224 , 7565 g − 215 , 1483 g × 100

  % Kadar air I = 224 , 7565 g

  = 4,27 % 224 , 7565 g − 214 , 9604 g × 100

  % Kadar air II = 224 , 7565 g

  = 4,35 %

• 4 ,

  27 % 4 , 35 % % Kadar air rata-rata = = 4,31%

  2

  Lampiran 9. (Lanjutan) b. perhitungan kadar air ekstrak

  Kode BE BW Oven 1 Oven 2 % SP Pabatu 5,0226 g 38,4016 g 43,0861 g 41,8578 g 2,19 Keterangan: BE = Berat esktrak, BW = Berat wadah

  W 1 − W2 % Kadar air= 100

  × W 1

  Keterangan: W1 = Berat ekstrak sebelum dioven W2 = Berat ekstrak setelah dioven 43,4242 g - 43,0861 g × 100

  % Kadar air I = 43 , 4242 g

  = 0,78 %

• 43,4242 g 41,8578g × 100

  % Kadar air II = 43 , 4242 g

  = 3,61 % 78 % + , 3 , 61 %

  % Kadar air rata-rata = = 2,19 %

  2

  Lampiran 9. (Lanjutan) c. perhitungan kadar asam lemak bebas (ALB) ekstrak

  Kode sampel Berat sampel

  B. Titrasi % ALB SP1 Pabatu 1,0404 g 3,1 ml 9,46 SP2 Pabatu 1,0019 g 3,4 ml

  VT × N ×BM asam palmitat

  % ALB=

  BS

  Keterangan: N = Normalitas KOH BM asam palmitat = 25,6 BS = Berat sampel

  VT = Volum titrasi × × 3 ,

  1 ml , 1161 N 25 ,

  6 % ALB SP1 =

  1 , 0404 g = 8,85 %

  3 , 4 ml × , 1161 N × 25 ,

  

6

% ALB SP2 =

  1 , 0019 g

  = 10,08 %

  85 % 10 , 08 % % ALB rata-rata = 9,46 %

• 8 ,

  =

  2 d. perhitungan nilai bilangan DOBI ekstrak

  

Kode sampel DOBI Rerata Deviasi

λ 446 λ 269 SP Pabatu 1 0,0319 0,0088

  3.63 3.63 0,00 0,0319 0,0088

  3.63 Absorbansi

  λ=446 nm

  DOBI =

  Absorbansi λ=269 nm

  , 0319 DOBI = = 3,63

  , 0088

  Lampiran 9. (Lanjutan) e.

  

Perhitungan kadar karotenoid di dalam ekstrak serat mesokarp kelapa sawit

Kode sampel Berat sampel WL 446 nm ppm Karoten

  

ELSMKS 0,0445 g 0,04456 3835

Keterangan : ELSMKS = ekstrak limbah serat mesokarp kelapa sawit Rumus: Total karotenoid (ppm) =

  B BM Abs V × ×

  Keterangan: V = Volume pengenceran Abs = Absorbansi BM = Berat molekul (karoten 383) B = Berat sampel yang ditimbang

  Total karotenoid ESMKS (ppm) = 0445 , , 383 04456

  10 × × =

  0445 , 170 6648 ,

= ppm 3835

  Lampiran 10. Perhitungan IC 50 ekstrak serat mesokarp kelapa sawit Absorbansi % peredaman Konsentrasi Abs 1 Abs 2 Abs 1 Abs 2 Rata-rata

  DPPH 1,01001 1,11523

0,5 ppm 0,95215 1,00623 5,72 9,77 7,75

2 ppm 0,81274 0,97241 19,53 12,80 16,17 4 ppm 0,65039 0,70581 35,60 36,71 36,16 8 ppm 0,4426 0,46826 56,17 58,01 57,1 10 ppm 0,40955 0,44531 60,64 60,07 60,36

  12 ppm 0,3501 0,37915 65,33 66,00 65,67 15 ppm 0,30298 0,31946 70,00 71,35 70,68 −

  Ablanko Asampel Rumus % peredaman = × 100

  Ablanko Data IC

  50

  2 X Y

  XY

  X

0,5 7,75 3,875 0,25

2 16,17 32,34

  4 4 36,16 144,64 16 8 57,1 456,8 64

10 60,36 603,6 100

  

12 65,67 788,04 144

15 70,68 1060,2 225

  2 ∑X= 51,5 ∑Y= 313,89 ∑XY= 3089,495 ∑X =553,25

  X = 6,44 Y = 39,23 Keterangan: = konsentrasi (ppm) dan Y= % peredaman

  Lampiran 10. (Lanjutan)

  XY

  X Y n − /

  ∑ ∑ ∑ = a

  2

  2 X X n − /

  ( ) ∑ ∑

  3089,495 − ( ) 51 , 5 ( 313,89 ) /

  8 =

  2 553 , 25 − 51 , 5 /

  8

( )

  3089,495 2020 , 667 − =

  553 , 25 331 ,

  

53

− 1068 ,

  83 =

  221 ,

  72 =

  4,82 Y = a X + b Y

  X b = - a b = 39,23 - (4,82) (6,44) b = 39,23 – 31,04 b = 8,19

Jadi persamaan garis regresi Y= 4,82 X + 8,19

Nilai IC

  50 = Y= 4,82 X + 8,19 50= 4,82 X + 8,19 X= 8,67

  IC

50 = 8,67

  ≈ 10 ppm

  Lampiran 11. Perhitungan variasi dosis berdasarkan IC 50 untuk sediaan krim ekstrak serat mesokarp kelapa sawit sebagai anti-aging Telah diketahui: Kadar karotenoid ekstrak = 3835 ppm, IC

  2 100 / 3835 /

  5 100 / 3835 / 200

= ×

  6. F6 = 200 ppm = g g g g g g

  µ µ

  3 100 / 3835 / 120

= ×

  5. F5 = 120 ppm = g g g g g g

  80 = × µ µ

  4. F4 = 80 ppm = g g g g g g

  50 = 8,67 ≈ 10 ppm untuk 100% antioksidan.

  40 = × µ µ

  

1 100

/ 3835 /

  3. F3 = 40 ppm = g g g g g g

  20 = × µ µ

  100 5 , / 3835 /

  1. F1 = Blanko (tanpa ekstrak) 2. F2 = 20 ppm = g g g g g g

  µ µ

  

Lampiran 12. Contoh perhitungan viskositas sediaan krim ekstrak serat

mesokarp kelapa sawit Rumus: Dial reading × factor = ..... centipoise/mPa.s

  Telah diketahui: - Spindel = 64

• Speed = 12
• Faktor perkalian = 500

  No Formula Pembacaan

  7

  14

  21

  28

  90

  1 F1

  27 27 26,5 25 24,5 19,5

  2 F2

  25 25 24,5

  23 22 19,5

  3 F3 21,5 21,5 20,5 20 19,5 17,5

  4 F4 19,5 19,5 19 18,5

  18

  13

  5 F5

  18

  18 18 17,5

  17

  12

  6 F6 17,5 17,5 17,5

  17

  17

  12

  1. F1 = 27 × 500 = 13500 cps = 135p

  2. F2 = 25 × 500 = 12500 cps = 125p

  3. F3 = 21,5 × 500 = 10750 cps = 107,5p

  4. F4 = 19,5 × 500 = 9750 cps = 97,5p

  5. F5 = 18 × 500 = 9000 cps = 90p

  6. F6 = 17,5 × 500 = 8750 cps = 87,5p

Keterangan: F: Formula, F1: blanko (tanpa ekstrak) dan sediaan krim ekstrak

serat mesokarp kelapa sawit F2: 0,5%, F3: 1%, F4: 2%, F5: 3%, F6: 5%

  

Lampiran 13. Hasil variansi (ANAVA) dan Tukey untuk pemulihan kulit mata

bagian lateral sukarelawan selama 4 minggu

a. Kadar air (Moisture) ANOVA Sum of Squares df Mean Square F Sig.

  Sebelum Between Groups 3.611 5 .722 2.600 .081 Within Groups 3.333 12 .278 Total 6.944

  17 MingguI Between Groups 15.833 5 3.167 8.143 .001 Within Groups 4.667 12 .389 Total 20.500

  17 MingguII Between Groups 17.111 5 3.422 6.844 .003 Within Groups 6.000 12 .500 Total 23.111

  17 MingguIII Between Groups 13.778 5 2.756 6.200 .005 Within Groups 5.333 12 .444 Total 19.111

  17 MingguIV Between Groups 18.278 5 3.656 8.225 .001 Within Groups 5.333 12 .444 Total 23.611

  17 Sebelum PEMULIHAN Subset for alpha = 0.05

  N

  1

  a

  Tukey HSD Krim Blanko 3 27,33 Krim 0,5% 3 28,00 Krim 1% 3 27,67 Krim 2% 3 28,67 Krim 3% 3 27,67 Krim 5% 3 28,33 Sig.

  ,339 Means for groups in homogeneous subsets are displayed.

  a. Uses Harmonic Mean Sample Size = 3,000.

  Lampiran 13. (Lanjutan) MingguI

  PEMULIHAN Subset for alpha = 0.05 N

  1

  2

  a

  Tukey HSD Krim Blanko 3 28,33 Krim 0,5% 3 30,00 30,00 Krim 1% 3 30,00 30,00 Krim 2% 3 30,67 Krim 3% 3 30,67 Krim 5% 3 31,33 Sig. ,0,58 ,166 Means for groups in homogeneous subsets are displayed.

  a. Uses Harmonic Mean Sample Size = 3,000.

  MingguII

  PEMULIHAN Subset for alpha = 0.05 N

  1

  2

  a

  Tukey HSD Krim Blanko 3 29,00 Krim 0,5% 3 30,67 30,67 Krim 2% 3 30,67 30,67 Krim 1% 3 31,00 Krim 3% 3 31,00 Krim 5% 3 32,33 Sig. ,290 ,506 Means for groups in homogeneous subsets are displayed.

  a. Uses Harmonic Mean Sample Size = 3,000.

  MingguIII

  PEMULIHAN Subset for alpha = 0.05 N

  1

  2

  3

  a

  Tukey HSD Krim Blanko 3 30,33 Krim 0,5% 3 31,00 31,00 Krim 1% 3 32,00 32,00 32,00 Krim 3% 3 32,00 32,00 32,00 Krim 2% 3 32,33 32,33 Krim 5% 3 33,00 Sig. ,109 ,109 ,538 Means for groups in homogeneous subsets are displayed.

  a. Uses Harmonic Mean Sample Size = 3,000.

  MingguIV

  PEMULIHAN Subset for alpha = 0.05 N

  1

  2

  a

  Tukey HSD Krim Blanko 3 30,33 Krim 0,5% 3 31,67 31,67 Krim 2% 3 32,33 32,33 Krim 1% 3 32,67 Krim 3% 3 33,00 Krim 5% 3 33,33 Sig. ,214 ,082 Means for groups in homogeneous subsets are displayed.

  a. Uses Harmonic Mean Sample Size = 3,000.

  Lampiran 13. (Lanjutan)

b. Kehalusan (Evenness) ANOVA

  Mean Square F Sig. Sebelum Between Groups 7,611 5 1,522 1,522 ,255

  ,214 Means for groups in homogeneous subsets are displayed.

  a. Uses Harmonic Mean Sample Size = 3,000.

  ,131 Means for groups in homogeneous subsets are displayed.

  Krim 5% 3 33,67 Krim 3% 3 33,67 Krim 2% 3 34,67 Krim 1% 3 35,33 Krim 0,5% 3 35,00 Krim Blanko 3 35,67 Sig.

  a

  1 Tukey HSD

  PEMULIHAN N Subset for alpha = 0.05

  MingguI

  a. Uses Harmonic Mean Sample Size = 3,000.

  Krim 5% 3 34,00 Krim 3% 3 35,00 Krim 2% 3 35,67 Krim 1% 3 35,67 Krim 0,5% 3 35,33 Krim Blanko 3 36,00 Sig.

  Within Groups 12,000 12 1,000 Total 19,611

  Sum of Squares df

  1 Tukey HSD

  Subset for alpha = 0.05

  17 Sebelum PEMULIHAN N

  17 MingguIV Between Groups 71,333 5 14,267 16,050 ,000 Within Groups 10,667 12 ,889 Total 82,000

  17 MingguIII Between Groups 41,333 5 8,267 9,300 ,001 Within Groups 10,667 12 ,889 Total 52,000

  17 MingguII Between Groups 36,944 5 7,389 6,045 ,005 Within Groups 14,667 12 1,222 Total 51,611

  17 MingguI Between Groups 15,778 5 3,156 2,272 ,113 Within Groups 16,667 12 1,389 Total 32,444

  a

  Lampiran 13. (Lanjutan) MingguII

  a

  Sig. ,086 ,538 ,086 Means for groups in homogeneous subsets are displayed.

  Krim 5% 3 28,67 Krim 3% 3 29,33 Krim 2% 3 30,33 30,33 Krim 1% 3 31,33 Krim 0,5% 3 31,67 31,67 Krim Blanko 3 34,00

  a

  3 Tukey HSD

  2

  1

  PEMULIHAN N Subset for alpha = 0.05

  MingguIV

  a. Uses Harmonic Mean Sample Size = 3,000.

  Sig. ,086 ,086 ,171 Means for groups in homogeneous subsets are displayed.

  Krim 3% 3 30,67 Krim 5% 3 31,67 31,67 Krim 2% 3 32,33 32,33 32,33 Krim 0,5% 3 33,67 33,67 Krim 1% 3 34,00 34,00 Krim Blanko 3 34,33

  3 Tukey HSD

  PEMULIHAN N Subset for alpha = 0.05

  2

  1

  PEMULIHAN N Subset for alpha = 0.05

  MingguIII

  a. Uses Harmonic Mean Sample Size = 3,000.

  Sig. ,053 ,053 ,475 Means for groups in homogeneous subsets are displayed.

  Krim 5% 3 32,00 Krim 3% 3 32,67 32,67 Krim 2% 3 33,67 33,67 33,67 Krim 0,5% 3 34,67 34,67 34,67 Krim 1% 3 35,00 35,00 Krim Blanko 3 35,33

  a

  3 Tukey HSD

  2

  1

  a. Uses Harmonic Mean Sample Size = 3,000.

  Lampiran 13. (Lanjutan)

c. Pori (Pore) ANOVA

  Sum of Mean Squares df Square F Sig. Sebelum Between Groups 198,444 5 39,689 13,230 ,000

  Within Groups 36,000 12 3,000 Total 234,444

  17 MingguI Between Groups 308,278 5 61,656 29,205 ,000 Within Groups 25,333 12 2,111 Total 333,611

  17 MingguII Between Groups 301,333 5 60,267 31,906 ,000 Within Groups 22,667 12 1,889 Total 324,000

  17 MingguIII Between Groups 373,778 5 74,756 26,912 ,000 Within Groups 33,333 12 2,778 Total 407,111

  17 MingguIV Between Groups 388,944 5 77,789 26,419 ,000 Within Groups 35,333 12 2,944 Total 424,278

  17 Sebelum PEMULIHAN Subset for alpha = 0.05

  N

  1

  2

  3

  a

  Tukey HSD Krim 5% 3 25,67 Krim 3% 3 29,00 29,00 Krim 2% 3 31,00 31,00 Krim 1% 3 33,33 33,33 Krim 0,5% 3 34,67 Krim Blanko 3 35,00

  Sig. ,245 ,082 ,119 Means for groups in homogeneous subsets are displayed.

  a. Uses Harmonic Mean Sample Size = 3,000.

  MingguI

  PEMULIHAN Subset for alpha = 0.05 N

  1

  2

  3

  a

  Tukey HSD Krim 5% 3 22,67 Krim 3% 3 29,00 Krim 2% 3 30,67 Krim 1% 3 32,67 32,67 Krim Blanko 3 34,67 Krim 0,5% 3 34,67

  Sig. 1,000 ,078 ,564 Means for groups in homogeneous subsets are displayed.

  a. Uses Harmonic Mean Sample Size = 3,000.

  Lampiran 13. (Lanjutan) MingguII

  a

  Sig. 1,000 ,114 ,078 Means for groups in homogeneous subsets are displayed.

  Krim 5% 3 18,67 Krim 3% 3 24,33 Krim 2% 3 28,33 28,33 Krim 1% 3 30,00 Krim 0,5% 3 30,33 Krim Blanko 3 32,67

  a

  3 Tukey HSD

  2

  1

  PEMULIHAN N Subset for alpha = 0.05

  MingguIV

  a. Uses Harmonic Mean Sample Size = 3,000.

  Sig. 1,000 ,100 ,214 Means for groups in homogeneous subsets are displayed.

  Krim 5% 3 19,33 Krim 3% 3 25,67 Krim 1% 3 29,67 29,67 Krim 2% 3 30,33 Krim 0,5% 3 31,33 Krim Blanko 3 33,00

  3 Tukey HSD

  PEMULIHAN N Subset for alpha = 0.05

  2

  1

  PEMULIHAN N Subset for alpha = 0.05

  MingguIII

  a. Uses Harmonic Mean Sample Size = 3,000.

  Sig. 1,000 ,095 ,358 ,238 Means for groups in homogeneous subsets are displayed.

  Krim 5% 3 22,00 Krim 3% 3 26,33 Krim 2% 3 29,67 29,67 Krim 1% 3 31,67 31,67 Krim 0,5% 3 32,00 32,00 Krim Blanko 3 34,33

  a

  4 Tukey HSD

  3

  2

  1

  a. Uses Harmonic Mean Sample Size = 3,000.

  Lampiran 13. (Lanjutan)

d. Noda (Spot) ANOVA

  Mean Square F Sig. Sebelum Between Groups 20,667 5 4,133 2,566 ,084

  a. Uses Harmonic Mean Sample Size = 3,000.

  a. Uses Harmonic Mean Sample Size = 3,000.

  Sig. ,314 ,076 Means for groups in homogeneous subsets are displayed.

  Krim 2% 3 25,00 Krim 5% 3 25,00 Krim 3% 3 25,33 25,33 Krim 1% 3 25,67 25,67 Krim 0,5% 3 27,33 27,33 Krim Blanko 3 28,67

  a

  2 Tukey HSD

  1

  PEMULIHAN N Subset for alpha = 0.05

  MingguI

  ,063 Means for groups in homogeneous subsets are displayed.

  Within Groups 19,333 12 1,611 Total 40,000

  Sum of Squares df

  a

  1 Tukey HSD

  Subset for alpha = 0.05

  17 Sebelum PEMULIHAN N

  17 MingguIV Between Groups 119,111 5 23,822 10,459 ,000 Within Groups 27,333 12 2,278 Total 146,444

  17 MingguIII Between Groups 83,611 5 16,722 13,682 ,000 Within Groups 14,667 12 1,222 Total 98,278

  17 MingguII Between Groups 45,833 5 9,167 8,684 ,001 Within Groups 12,667 12 1,056 Total 58,500

  17 MingguI Between Groups 33,833 5 6,767 3,929 ,024 Within Groups 20,667 12 1,722 Total 54,500

  Krim 5% 3 26,33 Krim 3% 3 26,33 Krim 2% 3 25,33 Krim 1% 3 26,00 Krim 0,5% 3 27,33 Krim Blanko 3 28,67 Sig.

  Lampiran 13. (Lanjutan) MingguII

  Krim 5% 3 22,33 Krim 3% 3 23,00 Krim 2% 3 23,33 Krim 1% 3 24,00 24,00 Krim 0,5% 3 26,67 26,67 Krim Blanko 3 28,33

  Sig. ,145 ,219 ,600 Means for groups in homogeneous subsets are displayed.

  Krim 5% 3 20,00 Krim 3% 3 21,33 Krim 2% 3 22,67 22,67 Krim 1% 3 22,33 22,33 Krim 0,5% 3 25,67 25,67 Krim Blanko 3 27,67

  a

  3 Tukey HSD

  2

  1

  PEMULIHAN N Subset for alpha = 0.05

  MingguIV

  a. Uses Harmonic Mean Sample Size = 3,000.

  Sig. ,475 ,098 ,475 Means for groups in homogeneous subsets are displayed.

  a

  PEMULIHAN N Subset for alpha = 0.05

  3 Tukey HSD

  2

  1

  PEMULIHAN N Subset for alpha = 0.05

  MingguIII

  a. Uses Harmonic Mean Sample Size = 3,000.

  Sig. ,129 ,129 Means for groups in homogeneous subsets are displayed.

  Krim 5% 3 24,33 Krim 3% 3 24,67 Krim 2% 3 24,00 Krim 1% 3 25,00 Krim 0,5% 3 26,33 26,33 Krim Blanko 3 28,67

  a

  2 Tukey HSD

  1

  a. Uses Harmonic Mean Sample Size = 3,000.

  Lampiran 13. (Lanjutan)

e. Keriput (Wrinkle) ANOVA

  Sum of Mean Squares df Square F Sig. Sebelum Between Groups 3,611 5 ,722 1,300 ,327

  Within Groups 6,667 12 ,556 Total 10,278

  17 MingguI Between Groups 19,778 5 3,956 3,956 ,058 Within Groups 12,000 12 1,000 Total 31,778

  17 MingguII Between Groups 51,333 5 10,267 6,600 ,004 Within Groups 18,667 12 1,556 Total 70,000

  17 MingguIII Between Groups 115,611 5 23,122 11,561 ,000 Within Groups 24,000 12 2,000 Total 139,611

  17 MingguIV Between Groups 145,333 5 29,067 32,700 ,000 Within Groups 10,667 12 ,889 Total 156,000

  17 Sebelum PEMULIHAN Subset for alpha = 0.05

  N

  1 Krim 5% 3 27,00 Krim 3% 3 27,00 Krim 2% 3 27,67 Krim 1% 3 28,00

  a

  Tukey HSD Krim 0,5% 3 28,00 Krim Blanko 3 28,00 Sig.

  ,589 Means for groups in homogeneous subsets are displayed.

  a. Uses Harmonic Mean Sample Size = 3,000. MingguI

  PEMULIHAN Subset for alpha = 0.05 N

  1 Krim 5% 3 25,33 Krim 3% 3 26,00 Krim 2% 3 27,67 Krim 1% 3 27,67

  a

  Tukey HSD Krim 0,5% 3 28,00 Krim Blanko 3 28,00 Sig.

  ,058 Means for groups in homogeneous subsets are displayed.

  a. Uses Harmonic Mean Sample Size = 3,000.

  Lampiran 13. (Lanjutan)

  a

  Sig. ,086 ,320 Means for groups in homogeneous subsets are displayed.

  Krim 5% 3 20,00 Krim 3% 3 22,33 Krim 2% 3 26,67 Krim 1% 3 26,33 Krim 0,5% 3 26,67 Krim Blanko 3 28,00

  a

  2 Tukey HSD

  1

  PEMULIHAN N Subset for alpha = 0.05

  a. Uses Harmonic Mean Sample Size = 3,000. MingguIV

  Sig. ,068 ,068 Means for groups in homogeneous subsets are displayed.

  Krim 5% 3 20,67 Krim 3% 3 24,33 24,33 Krim 2% 3 27,00 Krim 1% 3 27,00 Krim 0,5% 3 27,33 Krim Blanko 3 28,00

  2 Tukey HSD

  MingguII PEMULIHAN N

  1

  Subset for alpha = 0.05

  MingguIII PEMULIHAN N

  a. Uses Harmonic Mean Sample Size = 3,000.

  Sig. ,269 ,269 Means for groups in homogeneous subsets are displayed.

  Krim 5% 3 23,00 Krim 3% 3 25,33 25,33 Krim 1% 3 27,00 Krim 0,5% 3 27,33 Krim Blanko 3 27,67 Krim 2% 3 27,67

  a

  2 Tukey HSD

  1

  Subset for alpha = 0.05

  a. Uses Harmonic Mean Sample Size = 3,000.

  Lampiran 13. (Lanjutan)

f. Kedalaman keriput (Wrinkle’s Depth) ANOVA

  Sum of Mean Squares df Square F Sig. Sebelum Between Groups 76,944 5 15,389 8,147 ,001

  Within Groups 22,667 12 1,889 Total 99,611

  17 MingguI Between Groups 106,278 5 21,256 13,193 ,000 Within Groups 19,333 12 1,611 Total 125,611

  17 MingguII Between Groups 107,167 5 21,433 12,056 ,000 Within Groups 21,333 12 1,778 Total 128,500

  17 MingguIII Between Groups 167,111 5 33,422 31,663 ,000 Within Groups 12,667 12 1,056 Total 179,778

  17 MingguIV Between Groups 239,167 5 47,833 26,906 ,000 Within Groups 21,333 12 1,778 Total 260,500

  17 Sebelum

  a

  Tukey HSD PEMULIHAN Subset for alpha = 0.05

  N

  1

  2 Krim 5% 3 38,67 Krim 3% 3 41,00 41,00 Krim 0,5% 3 41,67 41,67 Krim 1% 3 43,67 Krim Blanko 3 44,00 Krim 2% 3 44,67 Sig. ,152 ,058 Means for groups in homogeneous subsets are displayed.

  a. Uses Harmonic Mean Sample Size = 3,000.

  MingguI

  PEMULIHAN Subset for alpha = 0.05 N

  1

  2

  a

  Tukey HSD Krim 5% 3 37,67 Krim 3% 3 41,33 Krim 0,5% 3 41,67 Krim 1% 3 44,00 Krim Blanko 3 44,33 Krim 2% 3 44,67 Sig. 1,000 ,063 Means for groups in homogeneous subsets are displayed.

  a. Uses Harmonic Mean Sample Size = 3,000.

  Lampiran 13. (Lanjutan) MingguII

  a

  Sig. 1,000 1,000 ,134 Means for groups in homogeneous subsets are displayed.

  Krim 5% 3 32,67 Krim 3% 3 37,00 Krim 0,5% 3 40,67 Krim 1% 3 41,33 Krim 2% 3 41,67 Krim Blanko 3 43,67

  a

  3 Tukey HSD

  2

  1

  PEMULIHAN N Subset for alpha = 0.05

  MingguIV

  Uses Harmonic Mean Sample Size = 3,000.

  a.

  Sig. 1,000 ,833 ,129 ,402 Means for groups in homogeneous subsets are displayed.

  Krim 5% 3 35,00 Krim 3% 3 39,67 Krim 0,5% 3 40,67 40,67 Krim 1% 3 42,67 42,67 Krim 2% 3 43,00 43,00 Krim Blanko 3 44,33

  4 Tukey HSD

  PEMULIHAN N Subset for alpha = 0.05

  3

  2

  1

  PEMULIHAN N Subset for alpha = 0.05

  MingguIII

  Uses Harmonic Mean Sample Size = 3,000.

  a.

  Sig. ,134 ,134 ,214 Means for groups in homogeneous subsets are displayed.

  Krim 5% 3 37,33 Krim 3% 3 40,33 40,33 Krim 0,5% 3 41,67 41,67 Krim 1% 3 43,33 43,33 Krim 2% 3 44,00 Krim Blanko 3 44,33

  a

  3 Tukey HSD

  2

  1

  a. Uses Harmonic Mean Sample Size = 3,000.

  

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