Uji Sensitivitas Staphylococcus aureus dan Escherichia coli terhadap Kotrimoksazol dalam Sediaan Tablet
UJI SENSITIVITAS Staphylococcus aureus DAN
Escherichia coli TERHADAP KOTRIMOKSAZOL DALAM
SEDIAAN TABLET
ABSTRAK
Kotrimoksazol merupakan kombinasi sulfametoksazol dan trimetoprim
dengan perbandingan 5 : 1. Spektrum kerja kotrimoksazol lebih luas
dibandingkan sulfonamida. Produk tablet kotrimoksazol tersedia dalam
berbagai nama dagang dari produsen yang berbeda. Efektivitas kotrimoksazol
terhadap bakteri dapat berubah selama distribusi dan penyimpanan, sehingga
perlu dievaluasi melalui pengujian secara mikrobiologi. Penelitian ini
dilakukan untuk menguji sensitivitas bakteri Staphylococcus aureus dan
Escherichia coli terhadap beberapa produk tablet kotrimoksazol.
Penelitian ini dilakukan terhadap lima produk tablet kotrimoksazol
yang diambil secara purposif. Sampel terdiri dari satu produk tidak bermerek
yang diberi kode A dan empat produk bermerek dengan kode masing-masing
B, C, D, dan E. Kelima produk tersebut dibuat dengan berbagai konsentrasi
dengan perbandingan trimetoprim : sulfametoksazol 25:125, 50:250, 100:500,
200:1000, 300:1500, 400:2000, 500:2500 µg/ml. Pengujian sensitivitas
Staphylococcus aureus dan Escherichia coli terhadap produk kotrimoksazol
dilakukan menggunakan metode Kirby-Bauer.
Hasil penelitian menunjukkan bahwa bakteri Staphylococcus aureus
dan Escherichia coli tidak sensitif (resisten) terhadap kelima produk
kotrimoksazol dan kotrimoksazol baku pada konsentrasi 25:125 µg/ml. Namun
pada konsentrasi 100:500 µg/ml, seluruh produk kotrimoksazol dan
kotrimoksazol baku efektif menghambat pertumbuhan Staphylococcus aureus
dan Escherichia coli dengan diameter zona hambat masing-masing
18,86 – 21,13 mm dan 18,10 - 20,13 mm. Analisis secara statistika
menggunakan uji Kolmogorov-Smirnov dan Kruskal-Wallis menununjukkan
bahwa produk kotrimoksazol A, B, C, D, dan E serta kotrimoksazol baku
memiliki mutu yang sama secara mikrobiologi (p>0,05).
Kata Kunci: Uji sensitivitas, kotrimoksazol, sulfametoksazol, trimetoprim,
Escherichia coli, Staphylococcus aureus
Universitas Sumatera Utara
SUSCEPTIBILITY TEST OF Staphylococcus aureus AND
Escherichia coli TO COTRIMOXAZOLE TABLET
ABSTRACT
Cotrimoxazole is a sulfamethoxazole and trimethoprim combination in
ratio of 1:5. The work spectrum of cotrimoxazole is broader than
sulfanilamide. Cotrimoxazole tablet is available in several branded names from
different manufacturers. The effectivity of cotrimoxazole againts bacteria can
be changed during distribution and storage, so it needs to be evaluated by
microbiological testing. This study was carried out to investigate the
susceptibility of Staphylococcus aureus and Escherichia coli to cotrimoxazole
tablets.
This study was conducted by collecting 5 cotrimoxazole tablet products
purposively. The samples consisted of an unbranded product which was
labelled as A and 4 branded products that were labelled as B, C, D, and E,
respectively. All of products were prepared by diluting cotrimoxazole at the
different concentrations of trimethoprim : sufametoxazole in ratio of 25:125,
50:250, 100:500, 200:1000, 300:1500, 400:2000, and 500:2500 μg/ml,
respectively. Susceptibility testing of Staphylococcus aureus and Escherichia
coli to cotrimoxazole products was evaluated using Kirby-Bauer method.
The results showed that Staphylococcus aureus and Escherichia coli
resistant to all of cotrimoxazole tablets and standard cotrimoxazole at the
concentration of 25:125 μg/ml. However, at the concentration of 100:500
μg/ml, cotrimoxazole tablets and standard cotrimoxazole effectively inhibited
the growth of Staphylococcus aureus and Escherichia coli with the diameter of
inhibition zone were 18.86 – 21.13 mm and 18.10 – 20.13 mm, respectively.
Statistical analysis using Kolmogorov-Smirnov and Kruskal-Wallis tests
indicated that the cotrimoxazole tablets A, B, C, D, E and standard
cotrimoxazole had microbiologically equal quality (p >0.05).
Keywords: Susceptibility
trimethoprim,
test,
cotrimoxazole,
sulfamethoxazole,
Escherichia coli, Staphylococcus aureus.
Universitas Sumatera Utara
Escherichia coli TERHADAP KOTRIMOKSAZOL DALAM
SEDIAAN TABLET
ABSTRAK
Kotrimoksazol merupakan kombinasi sulfametoksazol dan trimetoprim
dengan perbandingan 5 : 1. Spektrum kerja kotrimoksazol lebih luas
dibandingkan sulfonamida. Produk tablet kotrimoksazol tersedia dalam
berbagai nama dagang dari produsen yang berbeda. Efektivitas kotrimoksazol
terhadap bakteri dapat berubah selama distribusi dan penyimpanan, sehingga
perlu dievaluasi melalui pengujian secara mikrobiologi. Penelitian ini
dilakukan untuk menguji sensitivitas bakteri Staphylococcus aureus dan
Escherichia coli terhadap beberapa produk tablet kotrimoksazol.
Penelitian ini dilakukan terhadap lima produk tablet kotrimoksazol
yang diambil secara purposif. Sampel terdiri dari satu produk tidak bermerek
yang diberi kode A dan empat produk bermerek dengan kode masing-masing
B, C, D, dan E. Kelima produk tersebut dibuat dengan berbagai konsentrasi
dengan perbandingan trimetoprim : sulfametoksazol 25:125, 50:250, 100:500,
200:1000, 300:1500, 400:2000, 500:2500 µg/ml. Pengujian sensitivitas
Staphylococcus aureus dan Escherichia coli terhadap produk kotrimoksazol
dilakukan menggunakan metode Kirby-Bauer.
Hasil penelitian menunjukkan bahwa bakteri Staphylococcus aureus
dan Escherichia coli tidak sensitif (resisten) terhadap kelima produk
kotrimoksazol dan kotrimoksazol baku pada konsentrasi 25:125 µg/ml. Namun
pada konsentrasi 100:500 µg/ml, seluruh produk kotrimoksazol dan
kotrimoksazol baku efektif menghambat pertumbuhan Staphylococcus aureus
dan Escherichia coli dengan diameter zona hambat masing-masing
18,86 – 21,13 mm dan 18,10 - 20,13 mm. Analisis secara statistika
menggunakan uji Kolmogorov-Smirnov dan Kruskal-Wallis menununjukkan
bahwa produk kotrimoksazol A, B, C, D, dan E serta kotrimoksazol baku
memiliki mutu yang sama secara mikrobiologi (p>0,05).
Kata Kunci: Uji sensitivitas, kotrimoksazol, sulfametoksazol, trimetoprim,
Escherichia coli, Staphylococcus aureus
Universitas Sumatera Utara
SUSCEPTIBILITY TEST OF Staphylococcus aureus AND
Escherichia coli TO COTRIMOXAZOLE TABLET
ABSTRACT
Cotrimoxazole is a sulfamethoxazole and trimethoprim combination in
ratio of 1:5. The work spectrum of cotrimoxazole is broader than
sulfanilamide. Cotrimoxazole tablet is available in several branded names from
different manufacturers. The effectivity of cotrimoxazole againts bacteria can
be changed during distribution and storage, so it needs to be evaluated by
microbiological testing. This study was carried out to investigate the
susceptibility of Staphylococcus aureus and Escherichia coli to cotrimoxazole
tablets.
This study was conducted by collecting 5 cotrimoxazole tablet products
purposively. The samples consisted of an unbranded product which was
labelled as A and 4 branded products that were labelled as B, C, D, and E,
respectively. All of products were prepared by diluting cotrimoxazole at the
different concentrations of trimethoprim : sufametoxazole in ratio of 25:125,
50:250, 100:500, 200:1000, 300:1500, 400:2000, and 500:2500 μg/ml,
respectively. Susceptibility testing of Staphylococcus aureus and Escherichia
coli to cotrimoxazole products was evaluated using Kirby-Bauer method.
The results showed that Staphylococcus aureus and Escherichia coli
resistant to all of cotrimoxazole tablets and standard cotrimoxazole at the
concentration of 25:125 μg/ml. However, at the concentration of 100:500
μg/ml, cotrimoxazole tablets and standard cotrimoxazole effectively inhibited
the growth of Staphylococcus aureus and Escherichia coli with the diameter of
inhibition zone were 18.86 – 21.13 mm and 18.10 – 20.13 mm, respectively.
Statistical analysis using Kolmogorov-Smirnov and Kruskal-Wallis tests
indicated that the cotrimoxazole tablets A, B, C, D, E and standard
cotrimoxazole had microbiologically equal quality (p >0.05).
Keywords: Susceptibility
trimethoprim,
test,
cotrimoxazole,
sulfamethoxazole,
Escherichia coli, Staphylococcus aureus.
Universitas Sumatera Utara