Isolasi dan Karakterisasi Bakteri dan Jamur Pengkontaminasi Beberapa Kultur Jaringan dan Ruangan Kultur Departemen Biologi FMIPA USU

ISOLASI DAN KARAKTERISASI BAKTERI DAN
JAMURPENGKONTAMINASI BEBERAPA KULTUR
JARINGAN DAN RUANGAN KULTUR DEPARTEMEN
BIOLOGI FMIPA USU

ABSTRAK

Penelitian keanekaragaman bakteri dan jamur pengkontaminasikultur dan ruangan
Kultur Jaringan Tumbuhan Departemen Biologi FMIPA USU dan efisiensi
formalin 4% dalam mengurangi jumlah populasi bakteri pengkontaminan telah
dilakukan. Hasil penelitian menunjukkan bahwa genus bakteri dan jamur yang
diisolasi dari udara sama dengan yang diisolasi dari kultur eksplan yang
terkontaminasi.
Bakteri
pengkontaminasi
merupakanBacillussp.,
Staphylococcussp.,
Escherichiasp.,Streptococcussp.,
dan
Pseudomonassp.,sedangkan
jamur

pengkontaminasi
ialahAspergillussp.,
Fusariumsp., Mucorsp., Neurosporasp. dan Penicilliumsp.. Fumigasiruangan
dengan formalin 4% denjgan cara penyemprotan setiap minggu yang dilakukan
selama 6 minggu mampu menurunkan 50% jumlah bakteri pada hari pertama
setelah fumigasi dan hamper 100% jumlah bakteri sebelum difumigasi pada akhir
pengamatan (hari ke5 setelah fumigasi). Hasil ini menunjukkan bahwa perlakuan
fumigasi harus dilakukan secara berkala di Laboratorium Kultur Jaringan
Departemen Biologi FMIPA USU untuk menghindari terjadinya kontaminasi
kultur.

Kata Kunci : keanekargaman bakteri dan jamur pengkontaminan, kultur jaringan,
efisiensi fumigasi, formalin 4%

iv

Universitas Sumatera Utara

ISOLATION AND CHARACTERIZATION OF BACTERIA AND FUNGI
WHICH CONTAMINATED PLANT TISSUE CULTURES AND THE TISSUE

CULTURE LABORATORY DEPARTMENT BIOLOGY FMIPA USU

ABSTRACT

A study on the diversity of bacteria and fungi which contaminated plant tissue
cultures and the Plant Tisssue Culture Laboratory Department Biology FMIPA
USU and the efficiency of fumigation used formalin 4% for 6 weeks to reduce the
contaminants have been done. The results showed that the genus of bacteria and
fungal contaminants which isolated from the air was as same as isolated from the
plant tissue cultures contaminated.The bacterial contaminants were Bacillussp.,
Staphylococcussp., Escherichiasp., Streptococcussp. and Pseudomonassp.. The
fungal contaminants were Aspergillussp., Fusariumsp., Mucorsp., Neurosporasp.
and Penicilliumsp.. The fumigation in the room with spraying used formalin 4%
could reduce 50% bacteria on the first day after fumigation and nearly 100% as
same as the population bacteria before fumigation at the end of observation (the
5th day after fumigation). The results showed that fumigation should be done
regularly in the Tissue Culture Laboratory Department Biology FMIPA US to
prevent the contamination in the culture.

Keywords : Diversity of bacteria and fungi contaminants, tissue culture, efficiency

of fumigation, formalin 4%

v

Universitas Sumatera Utara