Penetapan Kadar Bahan Baku Betametason Valerat Dengan Metode HPLC (High Performance Liquid Chromatography)

Lampiran 1
Perhitungan Kadar Bahan Baku Betametason Valerat
Kadar bahan baku betametason valerat dihitung dengan rumus sebagai berikut:

AUC sp
× St BPFI %
AUC st
Keterangan:
AUC sp : Luas Area Sampel
AUC st
: Luas Area Standar
St BPFI % : Standar Baku Pembanding Primer
a. Data Luas Area Standar
No.
Sample Name
1.
Std. Betamethason 1
2.
Std. Betamethason 2
3.
Std. Betamethason 3

4.
Std. Betamethason 4
5.
Std. Betamethason 5
6.
Std. Betamethason 6
Mean
Std. Dev.
%RSD

Betamethason
348611
348752
349940
349690
349585
349510
349348
532
0,2

b. Data Luas Area Sampel
No.
Sample Name
1.
BB 1 (a)
2.
BB 1 (b)
Mean
Std. Dev.
%RSD

Betamethason
350001
349665
349833
237
0,1

• Kadar Bahan Baku Betametason valerat BB 1 (a)

=

AUC sp
× St BPFI %
AUC st

=

350001
× 100,917 %
349348

= 101,106%
• Kadar Bahan Baku Betametason valerat BB 1 (b)
=

AUC sp
× St BPFI %
AUC st

=

349665
× 100,917 %
349348

= 101,009%
Kadar rata − rata =

101,106 % + 101,009 %
= 101,057%
2

Kadar bahan baku betametason valerat adalah sebesar 101,057%

c. Standar Deviasi Larutan Standar Betametason Valerat BPFI
No.
1.
2.
3.

4.
5.
6.
∑
Rata-rata

SD = �
SD = �
SD = �

�
348611
348752
349940
349690
349585
349510
2096088
349348

Ʃ (� − �)²
n−1
1448226
6−1

1448226
5

SD = �289645,2
SD = 538,1
SD = 538

(� − �)
737
596
-592
-342
-237
-162
0

0

(� − �)²
543169
355216
350464
116964
56169
26244
1448226
241371

• % RSD (Relative Standard Deviation)
SD
× 100%
�
538
% RSD =
× 100%
349348

% RSD = 0,2%
% RSD =

d. Standar Deviasi Larutan Standar Betametason Valerat
No.
1.
2.
∑
Rata-rata

SD = �
SD = �
SD = �

�
350001
349665
699666
349833

(� − �)
-168
168
0
0

Ʃ (� − �)²
n−1
56448
2−1

56448
1

SD = √56448
SD = 237
• % RSD (Relative Standard Deviation)
SD
× 100%
�

237
× 100%
% RSD =
349833
% RSD = 0,1%

% RSD =

(� − �)²
28224
28224
56448
28224

Lampiran 2
Hasil Kadar Bahan Baku Betametason Valerat

Lampiran 3
Gambar alat-alat yang digunakan:
a. Alat HPLC (High Performance Liquid Chromatography)

Merk WATERS DETECTOR 2489, PUMP 1525.

b. Timbangan Analitik Digital (Digital Analitycal Balance)
Merk SARTORIUS-AG Tipe MSE225P-100-DU

c. Ultrasonic Digital
Merk ELMA Tipe D-78224