Syntax NPAR TESTS
K-W=koloni2 BY kel1 7 MISSING ANALYSIS.
Resources Processor Time
a
00:00:00.000 Elapsed Time
00:00:00.000 Number of Cases Allowed
112347 a. Based on availability of workspace memory.
[DataSet1] C:\Users\User\Documents\LAMORA FKG.sav
Kruskal-Wallis Test
Ranks
kel N
Mean Rank koloni2
100 5
13.00 50
5 13.00
25 5
13.00 12,5
5 13.00
6,25 5
28.00 Kontrol -
5 13.00
kontrol + 5
33.00 Total
35
Test Statistics
a,b
koloni2 Chi-Square
33.708 df
6 Asymp. Sig.
.000 a. Kruskal Wallis Test
b. Grouping Variable: kel
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NPar Tests Kruskal-Wallis Test
Ranks
kel N
Mean Rank koloni2
100 5
13.00 50
5 13.00
25 5
13.00 12,5
5 13.00
6,25 5
28.00 Kontrol -
5 13.00
kontrol + 5
33.00 Total
35
Test Statistics
a,b
koloni2 Chi-Square
33.708 Df
6 Asymp. Sig.
.000 a. Kruskal Wallis Test
b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 100
5 5.50
27.50 50
5 5.50
27.50 Total
10
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Test Statistics
b
koloni2 Mann-Whitney U
12.500 Wilcoxon W
27.500 Z
.000 Asymp. Sig. 2-tailed
1.000 Exact Sig. [21-tailed Sig.]
1.000
a
a. Not corrected for ties. b. Grouping Variable: kel
Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 100
5 5.50
27.50 25
5 5.50
27.50 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
12.500 Wilcoxon W
27.500 Z
.000 Asymp. Sig. 2-tailed
1.000 Exact Sig. [21-tailed Sig.]
1.000
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
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kel N
Mean Rank Sum of Ranks
koloni2 100
5 5.50
27.50 12,5
5 5.50
27.50 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
12.500 Wilcoxon W
27.500 Z
.000 Asymp. Sig. 2-tailed
1.000 Exact Sig. [21-tailed Sig.]
1.000
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 100
5 3.00
15.00 6,25
5 8.00
40.00 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
.000 Wilcoxon W
15.000 Z
-2.795 Asymp. Sig. 2-tailed
.005 Exact Sig. [21-tailed Sig.]
.008
a
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a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 100
5 5.50
27.50 Kontrol -
5 5.50
27.50 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
12.500 Wilcoxon W
27.500 Z
.000 Asymp. Sig. 2-tailed
1.000 Exact Sig. [21-tailed Sig.]
1.000
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 100
5 3.00
15.00 kontrol +
5 8.00
40.00 Total
10
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Test Statistics
b
koloni2 Mann-Whitney U
.000 Wilcoxon W
15.000 Z
-2.785 Asymp. Sig. 2-tailed
.005 Exact Sig. [21-tailed Sig.]
.008
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 50
5 5.50
27.50 25
5 5.50
27.50 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
12.500 Wilcoxon W
27.500 Z
.000 Asymp. Sig. 2-tailed
1.000 Exact Sig. [21-tailed Sig.]
1.000
a
a. Not corrected for ties. b. Grouping Variable: kel
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NPar Test Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 50
5 5.50
27.50 12,5
5 5.50
27.50 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
12.500 Wilcoxon W
27.500 Z
.000 Asymp. Sig. 2-tailed
1.000 Exact Sig. [21-tailed Sig.]
1.000
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 50
5 3.00
15.00 6,25
5 8.00
40.00 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
.000
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Wilcoxon W 15.000
Z -2.795
Asymp. Sig. 2-tailed .005
Exact Sig. [21-tailed Sig.] .008
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 50
5 5.50
27.50 Kontrol -
5 5.50
27.50 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
12.500 Wilcoxon W
27.500 Z
.000 Asymp. Sig. 2-tailed
1.000 Exact Sig. [21-tailed Sig.]
1.000
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
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koloni2 50
5 3.00
15.00 kontrol +
5 8.00
40.00 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
.000 Wilcoxon W
15.000 Z
-2.785 Asymp. Sig. 2-tailed
.005 Exact Sig. [21-tailed Sig.]
.008
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 25
5 5.50
27.50 12,5
5 5.50
27.50 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
12.500 Wilcoxon W
27.500 Z
.000 Asymp. Sig. 2-tailed
1.000 Exact Sig. [21-tailed Sig.]
1.000
a
a. Not corrected for ties. b. Grouping Variable: kel
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NPar Tests
Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 25
5 3.00
15.00 6,25
5 8.00
40.00 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
.000 Wilcoxon W
15.000 Z
-2.795 Asymp. Sig. 2-tailed
.005 Exact Sig. [21-tailed Sig.]
.008
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 25
5 5.50
27.50 Kontrol -
5 5.50
27.50 Total
10
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Test Statistics
b
koloni2 Mann-Whitney U
12.500 Wilcoxon W
27.500 Z
.000 Asymp. Sig. 2-tailed
1.000 Exact Sig. [21-tailed Sig.]
1.000
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests
Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 25
5 5.50
27.50 Kontrol -
5 5.50
27.50 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
12.500 Wilcoxon W
27.500 Z
.000 Asymp. Sig. 2-tailed
1.000 Exact Sig. [21-tailed Sig.]
1.000
a
a. Not corrected for ties. b. Grouping Variable: kel
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NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 25
5 3.00
15.00 kontrol +
5 8.00
40.00 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
.000 Wilcoxon W
15.000 Z
-2.785 Asymp. Sig. 2-tailed
.005 Exact Sig. [21-tailed Sig.]
.008
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 12,5
5 3.00
15.00 6,25
5 8.00
40.00 Total
10
Test Statistics
b
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koloni2 Mann-Whitney U
.000 Wilcoxon W
15.000 Z
-2.795 Asymp. Sig. 2-tailed
.005 Exact Sig. [21-tailed Sig.]
.008
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 12,5
5 5.50
27.50 Kontrol -
5 5.50
27.50 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
12.500 Wilcoxon W
27.500 Z
.000 Asymp. Sig. 2-tailed
1.000 Exact Sig. [21-tailed Sig.]
1.000
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
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Ranks
kel N
Mean Rank Sum of Ranks
koloni2 12,5
5 3.00
15.00 kontrol +
5 8.00
40.00 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
.000 Wilcoxon W
15.000 Z
-2.785 Asymp. Sig. 2-tailed
.005 Exact Sig. [21-tailed Sig.]
.008
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 6,25
5 8.00
40.00 Kontrol -
5 3.00
15.00 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
.000 Wilcoxon W
15.000 Z
-2.795 Asymp. Sig. 2-tailed
.005
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Exact Sig. [21-tailed Sig.] .008
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 6,25
5 3.00
15.00 kontrol +
5 8.00
40.00 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
.000 Wilcoxon W
15.000 Z
-2.619 Asymp. Sig. 2-tailed
.009 Exact Sig. [21-tailed Sig.]
.008
a
a. Not corrected for ties. b. Grouping Variable: kel
NPar Tests Mann-Whitney Test
Ranks
kel N
Mean Rank Sum of Ranks
koloni2 Kontrol -
5 3.00
15.00 kontrol +
5 8.00
40.00
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Ranks
kel N
Mean Rank Sum of Ranks
koloni2 Kontrol -
5 3.00
15.00 kontrol +
5 8.00
40.00 Total
10
Test Statistics
b
koloni2 Mann-Whitney U
.000 Wilcoxon W
15.000 Z
-2.785 Asymp. Sig. 2-tailed
.005 Exact Sig. [21-tailed Sig.]
.008
a
a. Not corrected for ties. b. Grouping Variable: kel
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DAFTAR PUSTAKA
1. Fatinos S.Panagos, Robin M.David. Gingival Disease- Their Etiologi Prevention and
Treatment. INTECH Open acess Publisher; 2011: 2-87. 2.
Preshaw P. Etiology of Periodontal disease. In: Newmen MG, Takei HH, Klokkwvold PR, Carranza FA. Eds. Carranza’s Clinical Periodontology, 11
th
ed. Philadelphia:St. Louis Elsevier, 2012:193-98 3.
Shalu Bathia. Periodontal Revisited. New Dehli:Jaypee Brothers Medical PublisherP LTD 2011:65-70.
4. Shantipriya Reddy. Essential Of Clinical Periodontology and Periodontis. New delhi:
Jaypee Brothers Medical Publisher P LTD 2011:65-70. 5.
Shagenobu kimura, Pathogenesis and Treatment of Periodontitis: INTECH Open acess Publisher 2011; 2-8.
6. Debby J. Suhanda.Gambaran Kebutuhan Perawatan Periodontal Pada Perokok Di Desa
Matungkas Kecamatan Dimembe.2015;31:108-09. 7.
Melak Aris Wahyukundari. Perbedaan Kadar MMF-8 Setelah Skalling dan Pemberian Tetrasiklin pada gingival crevicular Fluid Periodontitis Kronis. J PDGI 2008; 581: 1-6.
8. C.Aswini Somu. Efficiancy Of Herbal Of Herbal Extract Gel In The Treatment Of
Gingivitis: A clinical Study.J Ayur Integr Med 2012; 32 : 85-90. 9.
H Nilofer Farjana, SC Chandrasekaran, Bagadvad Gita. Effect of Oral Curcoma Gel In Gingivitis Management. J Clin Diagn Res 2014;812: 1-5.
10. Pertamuan Simanjumlah, STUDI Kimia dan Farmakologi Tanaman Kunyit Sebagai
Tubuhan Obat Serba Guna.J Kimia Mulawarna 2011;91:1-5. 11.
Raja Nivetha, Karthigeyan, Asuwks. The Efeect Of Natural Product Oral Health.Asian J Pharm Cli Res 2015; 71:1-4.
12. Madhu bhatia, Shisha sanesh. Anoval Therapatic Approachfor the Treatment of
Periodontic by Curcumin. J Clin Diagram 2014;812:1-5. 13.
S.Subasree, Karthikeyan,Sri Pradhas, Effect Of Tumeric on Oral Health. Int J Pharm Sci Health Care 2014;21:1-9.
14. H.S Grover, Himashhu Deswal, Amit Bhardwaj. Curcumin A medical Plant and Its
Effect In Medicine and Dentistry.Int J Contemporary Dental and Medical Review 2015;31:1-3.
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15. Sana Mukhtar. Antibacterial Activity Of Aqueous And Ethanololic Exracts Of Garlic
Cinnamon And Turmeric Against Escherichia Coli And Basillus Subtilitis. J Applied Biology and Pharmaceutical Technology 2012;31:131-136.
16. Akmane. Anaerobic Bacteria in Subject with Chronic Periodontitis and Periodontal
Health. JOHCD 2009;33:49-51 17.
Reshman Suvarna. Antibacterial Activity of Tumeric Against Entrococus Feacealis.Int J Curr.microbio 2014;32: 448-504.
18. Praveenkumar S. Mandroli. An in-vitro Evaluation of Antibacterial Activity Of
Curcumin Against Comman Endodontic Bacteria. J Pharm Sci 2013;310:106-108. 19.
Universal taxonomic service. Taxon: porphromonas gingivalis. http:www.uniprot.orgtaxonomy837.28 januari 2016.
20. Astir I. periodontitis kronis. Htt:www.scribd.comdoc134494473A januari 28 2016.
21. Muborokah S.outer membrane protein 49,4 Kda dari Porphromonas gingivalis
merupakan protein Hemaglutinin dan Adhesin terhadap Netrofil. J Kedokteraan Brawijaya 2009;252:49-59.
22. Alexa M.G The impact of virulence factors of Porphyromonas Gingivalis on wound
healing in vitro. J oral microbiology 2015; 71:1-8. 23.
Morten Enerson. Porphyromonas gingivalis Fimbriae. Journal of oral microbiology;2013:1-8.
24. BM Eley.TD Monson Periodontics Fifth Edition Wright IMPRINTED of Elevier Ltd:
2004, 8-10. 25.
Valerie Clerhugh. Aradna Tugnait. Periodontology at a Glance: Willey Blackwell Publication; 2009:1-3.
26. Dakshita Jay Sharma. Comparision of Antibacterial Effect of Propolis,Neem, Tea
Tree Oil, turmeric and Sodium Hypochrite. On Candida Albicans Biofilm.J Pharm Biomed science 2015;56:469-74.
27. Amita. Comparative Evalution Of 0.1 Turmeric Mouthwash With 0.2 Chlorohexide
Gluconate In Prevention Of Plaque And Gingivitis A Clinical And Microbiological Study. J Ind Soc 2012; 163:386-391.
28. Nayyar Nadhini.
Comparative evaluation of 1 Curcumin solution and 0.2 Chlorhexidine Irrigation As An Adjunct To Scaling And Root Planing In Management Of
Chronic Periodontitis: A clinico-microbiological study. JPBMS 2012;145:1-7.
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29. Sruthma. Effectiveness Of Sub Gingival Irrigation An Indigenous 1 Curcumin
Solution On Clinical Microbiology Parameter Chronic Periodontitis Patients: A Pilot And Rondamized Clinical Trial. CCD 2013;42:185-191.
30. Sandeep A. Lawande. Therapeutic Application Of Tumeric In Dentistry A Promising
Future. J Pharm Biomed Sci 2013; 2727: 580-91. 31.
Sharmila Devi,Prassanah Neelkantan. Curcoma Pharmacological Action and Its Role in Dentistry,APJRC;91:1-4.
32. Tiwarani Ranjana,Trisaathi. Theraupathic Effect Of Haridra In General Oral
Health.AYUSHARA 2014;12:40-5. 33.
S.Subasree, Karthikeyan,Sri Pradhas, Effect Of Tumeric on Oral Health. International Journal Of Pharmacuetical Science And Health Care 2014;21:1-9.
34. Ashok Kumar Population,Bangrahan Pagala. Extraction Of Curcumin From Tumeric
Root.IJRIS 2015;21:1-11. 35.
Nita Chainani. Safety Inflammatary Activity Of Curcumin A component Of Tumeric.JACM2003;91:101-08.
36. Prianca Madi Reddi. A Touch Of Tumeric Examming an Ayuvedic Tresure.Science
Research 2013;31: 91-5. 37.
Duggi Shrishali, Handrak Harish, Handrak Ravichandra. Tumeric Natural Preceious Medicine. Asia J Pharm Clin Res.2013;61:1-7.
38. Prof. Dr Mithra. Evaluation of Antimicrobial Activity of Aqueous and Hydro-
Alcoholic Curcoma Longa Extract against Endodontic Pathogens. J Pharm 2012; 22: 192-198.
39. Shagufta Naz. Antibacterial Activity of Curcuma Longa Variety against Different Strain
Of Bacterial. Pak J Bot 2010 ;421: 455-62.
40. Najah A.Mohammed. Evaluation Of Antimicrobial Activity of Curcumin against Two
Oral Bacteria. Auto Contr Intel Sys 2015;31-2:18-20.
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BAB 3 METODOLOGI PENELITIAN
3.1 Jenis Penelitian
Penelitian ini adalah penelitian kuasi eksperimental laboratorium dengan rancangan penelitian post test only control group design dan dilaksanakan secara in vitro.
3.2 Tempat dan Waktu Penelitian 3.2.1 Tempat Penelitian :
1. Laboratorium Obat Tradisional Fakultas Farmasi USU
2. Laboratorium Biologi Oral Universitas Airlangga
3.2.2 Waktu Penelitian : Januari 2016-Februari 2016
3.3 Sampel dan Besar Sampel Penelitian 3.3.1 Sampel Penelitian
Koloni Porphyromonas gingivalis ATCC 33277 yang telah diisolasi dan dibiakkan dalam media Nutrient Agar NA.
3.3.2 Besar Sampel Penelitian
Penentuan besar sampel dilakukan berdasarkan SOP Standard Operational Procedure di Laboratorium Biologi Oral Universitas Airlangga. Jumlah pengulangan yang
dilakukan pada penelitian ini menggunakan rumus Federer yaitu:
Keterangan: t = jumlah kelompok perlakuan dalam penelitian
r = banyak replikasi perlakuan ulang Pada penelitian ini dilakukan 5 perlakuan, yakni ekstrak kunyit dengan konsentrasi
100, 50, 25, 12,5, dan 6,25. Oleh karena itu, banyak replikasi pada penelitian ini adalah sebagai berikut:
t-1r-1 ≥ 15
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t-1r-1 ≥ 15
5-1r-1 ≥ 15
4 r-1 ≥ 15
r-1 ≥ 3,75
r ≥ 4,75
Jumlah perlakuan ulang r yang digunakan dalam penelitian ini adalah 5 kali perulangan.
a. Penentuan nilai KHM
− Kelompok I
: ekstrak dengan konsentrasi 100 = 5 sampel −
Kelompok II : ekstrak dengan konsentrasi 50 = 5 sampel
− Kelompok III : ekstrak dengan konsentrasi 25
= 5 sampel −
Kelompok IV : ekstrak dengan konsentrasi 12,5 = 5 sampel −
Kelompok V : ekstrak dengan konsentrasi 6,25 = 5 sampel −
Kelompok VI : kontrol Mc Farland = 5 sampel
Jumlah sampel = 30 sampel
b. Penentuan nilai KBM
− Kelompok I
: ekstrak dengan konsentrasi 100 = 5 sampel −
Kelompok II : ekstrak dengan konsentrasi 50 = 5 sampel
− Kelompok III : ekstrak dengan konsentrasi 25
= 5 sampel −
Kelompok IV : ekstrak dengan konsentrasi 12,5 = 5 sampel −
Kelompok V : ekstrak dengan konsentrasi 6,25 = 5 sampel −
Kelompok VI : kontrol Mc Farland = 5 sampel
Jumlah sampel = 30 sampel
3.4 Variabel Penelitian 3.4.1 Variabel Bebas
