The use of Mangrove Crab (Scylla sp.) shell chitosan as heavy metal lead (Pb) adsorbent - repository civitas UGM
Content Conference Report of IBSC2014 Organizing Committee..........................................................................
1 Committee - National Organizing Committee.......................................................................................
2 - International Advisory Committee....................................................................................
2 - Scientific Program Committee.........................................................................................
2 - Reviewers ……………………………………………………………………………………….
3 - Local Organizing Committee ………………………………………………………………….
4 List of Titles and Authors............................................................................................................................
5 Full Proceedings Track 1: Plant Animal and Environmental Science …………………………....................................
12 Track 2: Engineering and Economy in Agriculture ………………………….....................................
90 Track 3-1: Biotechno logy……………………………………………………........................................ 114
Track 3-2: Food Science & Technology.......................................................................................... 242 Track 4: Bioactive natural products …………………………………………....................................... 299 Track 5: Bio-Sensing Technology and Biomaterials …………………………................................... 375 IBSC2014 proceedings are published in electronic form only.
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Conference Report of IBSC2014 Organizing Committee
On behalf of the organizing committee of The International Bioscience Conference th and the 5 Joint international PSU-UNS Bioscience Conference 2014 between the Prince of Songkla University, Thailand and the University of Novi Sad, the Republics of Serbia, the Prince of Songkla University is delighted to host this Conference in 2014. The International Bioscience Conference is organized every two years to enhance the collaboration among various bioscience disciplines and hence help to st th shape the science of the 21 century together. This year, the 5 International
Bioscience Conference 2014 has its main theme “Achieving Sustainability through Integrated Sciences” and takes place on 29-30 September, 2014, in Phuket, Thailand
Top research workers from the Prince of Songkla University, the University of Novi Sad and other research scientists worldwide are gathering to share their experiences of bioscience research.
There are ninety-three papers from both national and international institutes, to share their knowledge. Hopefully, this conference will encourage and inspire young scientists to develop and strengthen their research for the benefits of mankind. Finally, I would like to express a deep appreciation to our main sponsor the Petroleum Authority of Thailand for their generous support.
Thank you, Associate Professor Dr. Wilaiwan Chotigeat The President of the Organizing Committee of The IBSC2014
Committee National Organizing Committee
1. Assoc. Prof. Dr. Chusak Limsakul President
2. Prof. Dr. Amonrat Phongdara Vice President
3. Assoc. Prof. Dr. Chutamas Satasook Vice President
4. Assoc. Prof. Dr. Wilaiwan Chotigeat
5. Asst. Prof. Taweesak Niyombandith
6. Asst. Prof. Dr. Sirirat Pinsuwan
7. Assoc. Prof. Dr. Wirote Youravong
8. Assoc. Prof. Dr. Anchana Prathep Secretariat
International Advisory Committee
1. Prof. Dr. Miroslav Vesković President
2. Prof. Dr. Neda Mimica- Dukivić
3. Prof. Dr. Slobodanka Pajević
4. Prof. Dr. Saša Orlović
5. Prof. Dr. Branko Ćupina
6. Prof. Dr. Dubravka Štajner
7. Prof. Dr. Jovan Matović 8.
Dr. Anamarija Mandić
9. Dr. Marija Lesjak
10. Miss Gordana Vlahović
Scientific Program Committee
1. Prof. Dr. Supayang Voravuthikunchai Chair of Committee
2. Assoc. Prof. Dr. Anchana Prathep Head of Track 1
3. Dr. Kobchai Worrapimphong Head of Track 2
4. Asst. Prof. Dr. Warapond Wanna Head of Track 3
5. Assoc. Prof. Dr. Wirote Youravong Head of Track 3
6. Assoc. Prof. Dr. Juraithip Wungsintaweekul Head of Track 4
7. Professor Dr. Vatcharin Rukachaisirikul Head of Track 4
8. Assoc. Prof. Dr. Proespichaya Kanatharana Head of Track 5
9. Assoc. Prof. Dr. Panote Thavarungkul Head of Track 5
10. Asst. Prof. Dr. Suda Chakthong Secretariat
Reviewers Track 1: Plant Animal and Environmental Science
1. Assoc. Prof. Dr. Anchana Prathep
2. Assoc. Prof. Dr. Pornsilp Pholpunthin
3. Dr. James Dominic True
4. Mr. Kitsanai Charoenjit
5. Dr. Raymond James Ritche
6. Asst. Prof. Dr. Jarun Leerativong
7. Dr. Jaruwat Nabhitabhata
8. Prof. Dr. Slobodanka Pajević
Track 2: Engineering and Economy in Agriculture
1. Dr. Kobchai Worrapimphong
2. Assoc. Prof. Dr. Buncha Somboonsuke
3. Assoc. Prof. Dr. Ayut Nissapa
4. Assoc. Prof. Dr. Yutthana Tirawanichakul
5. Asst. Prof. Dr. Chinnawat Surussavadee
6. Prof. Dr. Branko Ćupina
Track 3: Biotechnology and Food Science & Technology
1. Prof. Dr. Amornrat Phongdara
2. Assoc. Prof. Dr. Wilaiwan Chotigeat
3. Asst. Prof. Dr. Warapond Wanna
4. Dr. Monwadee Wonglapsuwan
5. Assoc. Prof. Dr. Duangporn Kantachote
6. Assoc. Prof. Dr. Prasert Suntinanalert
7. Assoc. Prof. Dr. Nongporn Towatana
8. Assoc. Prof. Dr. Sa-ad Riyajan
9. Assoc. Prof. Dr. Pakamas Chetpattananon
10. Dr. Yanisa Laoong-u-thai
11. Prof. Dr. Soottawat Benjakul
12. Asst. Prof. Dr. Mutita Meenune
13. Assoc. Prof. Dr. Suppasil Maneerat
14. Assoc. Prof. Dr. Wirote Youravong
15. Dr. Supachai Pisuchpen
16. Assoc. Prof. Dr. Aran Hanpongkittikun
17. Prof. Dr. Dubravka Štajner
Track 4: Bioactive natural products
1. Assoc. Prof. Dr. Juraithip Wungsintaweekul
2. Assoc. Prof. Dr. Anuchit Plubrukarn
3. Dr. Sukanya Dej-adisai
4. Asst. Prof. Dr.Sirirat Pinsuwan
5. Assoc. Prof. Dr. Pharkphoom Panichayupakaranant
6. Assoc. Prof. Dr. Roongnapa Srichana
7. Assoc. Prof. Dr. Ruedeekorn Wiwattanapatapee
8. Professor Dr. Vatcharin Rukachaisirikul
9. Assoc. Prof. Dr. Souwalak Phongpaichit
10. Dr. Kwanruthai Tadpetch
Reviewers Track 5: Bio-Sensing Technology and Biomaterials
1. Assoc. Prof. Dr. Proespichaya Kanatharana
2. Assoc. Prof. Dr. Panote Thavarungkul
3. Asst. Prof. Dr. Waraporn Limbut
4. Asst. Prof. Dr. Chongdee Thammakhet
5. Asst. Prof. Dr. Chittanon Buranachai
6. Dr. Chutintorn Punwong
7. Dr. Apon Numnuam
Local Organizing Committee
2. Asst. Dr. Kamontam Umsakul
10. Miss Janewadee Hiranrat
3. Dr. Phuvadol Thanakiatkrai
9. Mrs. Nattaya Kanhajaroenpon
4. Dr. Sureerat Homhuan
12. Mrs. Jittima Phosena
5. Mr. Komwit Surachat
13. Miss Thidawan Saelao
6. Miss Kamontorn Prompitak
14. Miss Wassamon Meesen
7. Miss Nittaya Raknun
15. Mr. Isarapab Chumruksa
8. Miss Tharita Sattayarak
16. Mr. Wanicbut Wattanamatiphot
1. Asst. Prof. Dr. Pattara Aiyarak
11. Miss Kusuma Achirasena
List of Titles and Authors Track 1: Plant Animal and Environmental Science No. Title Page
Phytoextraction of elevated heavy metals in soil by using fast Growing trees 13-18 (Salix sp. and Populus sp.)
T1-I1
Authors: Slobodanka Pajević*, Milan Borišev, Nataša Nikolić, Jadranka Luković, Milan Župunski, Danijela Arsenov, Saša Orlović
Forest ecosystems in Serbia 19-22
- –monitoring and adaptation T1-I2
Authors: Saša Orlović*, Zoran Galić, Andrej Pilipović, Srdjan Stojnić, Milan Drekić, Dejan Stojanović
Physiological and yield responses of yam (Dioscorea rotundata -Poir) vine cuttings 23-27
T1-O4 to varying rooting chemicals
Authors: Olusola Jacob Oyetunji, Ezekiel Taiwo Afolayan*
Use of chitosan of shrimp Penaeus sp. as cadmium adsorbent 28-30 T1-O6
- Authors: Swara Yudhasasmita
The use of Mangrove Crab (Scylla sp.) shell chitosan as heavy metal lead (Pb) 31-34 adsorbent T1-O7
Authors: Ilma Fistannisa Zette* , Andhika Puspito Nugroho
Arsenic determination in human hair in Ron Phibun district, Nakhon Si Thammarat 35-39 T1-O8 province by hydride generation atomic absorption spectrometry
Authors: Nucharee Juansai, Thitima Rujiralai, Wilairat Cheewasedtham*
Effects of organic fertilizers on uptake and accumulation of cadmium in Merigold 40-43 T1-O9 (Tagetes erecta L.)
Authors: Natthapong Pechampai, Weeradej Meeinkuirt*, Theerawut Phusantisampan, Jittawan Kubola
Species Composition and Host Specificity of Fig Wasps in Ficus schwarzii Koord. 44-47 T1-O11
Authors: Jirapan Yimkaew*, Chutamas Satasook
Seaweed and seagrass mapping in Thailand measured by using landsat 8 optical 48-52 T1-O14 properties c
Authors: Satomi Kakuta*, Wataru Takeuchi, Anchana Prathep
Vitamin E role as heavy metal (cadmium chloride) antitoxic on albino male rat 53-56 (Rattus norvegicus, Berkenhout, 1769) hepatic cells
T1-O21
Authors: Alfian Silvia Krisnasari*, Selvi Rahmawati, Qonita Shalihah Firdausyiah, Swara Yudhasasmita,
Dita NurtjahyaRecruit pattern, mortality and exploitation ratio values of Rhinoclavis sinensis 57-60 Gmelin 1791 (Gastropoda: Cerithiidae) from Krakal Beach, Yogyakarta, Indonesia
T1-P2
Authors: Felicia Zahida*, Jusup Subagja, Jesmandt Situmorang, Kamiso H. Nitimulyo
Photosynthetic performance carbohydrate contents and yield affected by kaolin 61-65 T1-P6 particle film application in grape
Authors: Teerapat Tepkaew, Sutsawat Duangsrisai, Pilanee Vaithanomsat, Kanapol Jutamanee*
Induction of polyploid in in vitro shoot of vetiver grass by colchicine for improving 66-70 T1-P7 salt tolerance
Authors: Mattanaporn Maikami, Lily Kaveeta, Surin Peyachoknagul, Malee Nanakorn*
Effect of initial pH and alkalinity on hydrogen production from palm oil mill effluent 71-75 by thermophilic batch fermentation T1-P8
Authors: Sukonlarat Chanthong*, Sompong O-Thong, Poonsuk Prasertsan
Track 1: Plant Animal and Environmental Science
No. Title Page
Effect of magnetic irrigation on plant height and seed protein of mung bean 76-78 T1-P9 (Vigna radiata L.) under water stress conditions
Authors: Omid Sadeghipour*
Screening of RR141 decolorizing bacteria from Khao Yai National Park, Thailand 79-83 T1-P13
Authors: Chalermwoot Sompark, Theerachai Thananata, Niramol Sakkayawong*
Point and non-point sources of pollution of Tisza River (Serbia) 84-89 T1-P14
Authors: Srdjan Kolakovic, Svetlana Vujovic*, Borislav Masic, Slobodan Kolakovic,Goran Jeftenic Track 2: Engineering and Economy in Agriculture
No. Title Page
Contribution of Phytocrene palmata to the Research and Development of plant 91-94 ingredients for the cosmetic industry
T2-01
Authors: Eric Gardette*, Pramoth Kheowvongsri, Theerayut Meelap
Effect of sludge pretreatment methods to improve methane production from palm oil 95-98 T2-02 mill effluent
Authors: Wiyada Khangkhachit*, Wiriya Duangsuwan, Poonsuk Prasertsan
Potential tuber of wild type banana (Musa acuminata Colla and Musa balbisiana 99-102 T2-03 Colla) for alternative food and bioethanol
Authors: Zanne Sandriati P.*, Ruly Budiono, Moh. Nurzaman
Potential of heat recovery from air compressor and benefits of its utilization for a 103-109 greenhouse heating T2-P1
Authors: Milovan Medojević* , Jovan Petrović, MilanaPerić
The potential use of agricultural biomass in order to satisfy energy needs of 110-113 T2-P2 municipal building
Authors: Milana Perić*, Milovan Medojević, Marko Pavlov Track 3-1: Biotechnology
No. Title Page
Isolation and characterization of potential probiotic bacteria for swine feeding 115-118 T3-OB4
Authors: Uriwan Parneesong, Kornkamol Udomsri, Kesinee Pluamjai, Natchanok Pachara, Kamoltip Promnares*
Comparative study of 2,3-butanediol production in C5 and C6 sugar by 119-122 T3-OB7 solventogenic Clostridium spp.
Authors: Chompunuch Glinwong, Jantarush Comwien, Angwara Vudthi, Warawut Chulalaksananukul*
Optimizing oleaginous biomass production of Yarrowia lipolytica Po1g for biodiesel 123-131 T3-OB8 feedstock
Authors: Thu T. Phan* , Thien D. Tran, Huong T. Nguyen
Optimization of harvesting process and characterization of marine Chlorella sp. 132-136 T3-OB9 biomass
Authors: Yohanis Irenius Mandik, Benjamas Cheirsilp*
Track 3-1: Biotechnology
No. Title Page
Cultivation of oleaginous fungi Aspergillus tubingensis TSIP9 on palm empty fruit 137-141 T3-OB10 bunches for enzymes and lipid production
Authors: Chonikan Junied, Benjamas Cheirsilp*
Cultivation of oleaginous microalgae for carbon dioxide fixation and lipid production 142-145 T3-OB11
Authors: Tipawan Thawechai, Benjamas Cheirsilp*
Optimization of hydrogen production from crude glycerol by Chlorella sp. 146-149 T3-OB12
Authors: Dennapa Sengmee*, Benjamas Cheirsilp
The effect of heat treatment on physical-chemical characteristics of chitosan 150-153 * T3-OB13
Authors: Goragot Supanakorn, Sorada Kanokpanont
Evaluation of oxidative stability and biological activities of liposome-encapsulated 154-158 T3-PB24 curcumin
Authors: Panchika Prangkio*, Supamas Supasri
Effect of Phagocytosis Activating Protein (PAP) gene against Aeromonas 159-162 T3-PB25 hydrophila in Cyprinus carpio by oral immunization
Pataraporn Kongmee*, Naraid Saunyuk, Panchalika Dechamag, Wilaiwan Chotigeat*
Graphene-conducting polymer nanocomposite modified electrochemical sensor for 163-166 free radical scavenger screening of ascorbic acid T3-PB26
Authors: Ruksuda Tirawattanakoson, Poomrat Rattanarat, Nadnudda Rodthongkum*, Nattaya Ngamrojanavanich*, Orawon Chailapakul*
Physicochemical and antioxidant properties of curry leaves (Murraya koenegii) 167-171 chutney powder T3-PB27
Authors: Palanivel Ganesan*, Yogeswari Murugan, Anuthida Phaiphan, Badlishah Sham Baharin
Characterization of alternative transcript of the 14-3-3 epsilon gene 172-175 T3-PB28
Authors: Chutintorn Chauywong, Warapond Wanna*
The optimization of primary cell culture systems from the ovarian and lymphoid 176-179 T3-PB29 organ of Banana shrimp
Authors: Nathakan Jaturonlak, Wilaiwan Chotigeat*
Study of cytokine gene expression during chikungunya virus infection 180-183 T3-PB30
Authors: Pattra Chalaem, Kamoltip Promnares* Induction of ovarian maturation in Banana shrimp by Emilia sonchifolia (L.) DC.
184-187 T3-PB31 plant extract
Authors: Lamai Maikaeo, Wilawan Mahabusarakam, Wilaiwan Chotigeat*
Conversion of pentoses, hexoses and disaccharide to be acetone-butanol-ethanol 188-192 (ABE) by Clostridium sp. Hdi
T3-PB32
Authors: Supattra Lertsriwong, Nassapat Boonvitthya, Warawut Chulaluksananukul, Chompunuch Glinwong*
Screening of lipase-producing thermophilic bacteria from hot springs in northern 193-197 T3-PB33 Thailand
Authors: Sukumarn Kittivisarn, Tikamporn Yongvanich, Warawut Chulalaksananukul*
Optimization of biodiesel production by mutant catalytic cells of Candida 198-202 orthopsilosis from crude palm oil using Response Surface Methodology T3-PB34
Authors: Wannisa Pansuk, Marisa Jatupornpiput, Aree Rittiboon*
Functional analysis of shrimp 14-3-3 protein involved in apoptosis 203-206 T3-PB35
Authors: Kittisak Thongpijit, Warapond Wanna*
Track 3-1: Biotechnology
No. Title Page
Adsorption of iron(III) and copper(II) by bacterial cellulose produced from 207-210 T3-PB36 Rhodococcus sp. MI 2
Authors: Pariyaporn Yingkong, Somporn Tanskul*
Dust mites detection by multiplex PCR 211-214
T3-PB37
Authors: Thanyarat Teerawattananon, Sirinthra Rittipong, Thatsanee Thet-Em, Nat Malainual*
Using crude glycerol for growth and lipid production by Mortierella elongata NR06 215-218 T3-PB38
Authors: Supavadee deelai, Panida Unagul, Niramol Sakkayawong*
Polyhydroxyalkanoates production from municipal wastes by Bacillus licheniformis 219-221 T3-PB39
Authors: Kanokphorn Sangkharak*, Poonsuk Prasertsan
The production of cholesterol-lowering agent (mevastatin) by solid-state cultivation 222-225 T3-PB40 of Monascus sp. SS14
Authors: Pharita Thongsuknok, Somchai Krairak*
Structural analysis and heterologous expression of human ROBO1 226-229 T3-PB41
Authors: Kanokporn Boonsirichai*, Suwimol Jetawattana
Design and testing of long throat venturi- and jet-type bubble generators for using in 230-233 T3-PB42 palm oil industry
Authors: Wiriya Duangsuwan*, Poonsuk Prasertsan, Annop Chawalitsittikul
Detection of oil palm variety tenera by Loop-Mediated Isothermal Amplification 234-237 T3-PB43 Method (LAMP)
Authors: Sarawut wannapat*, Alisa Nakkaew, Amornrat Phongdara
Development of a simple test for detecting the White Spot Syndrome Virus (WSSV) 238-241 T3-PB44
- Authors: Nittaya Bunsanong, Benyapa Intharasiri, Piyaporn Khunthongkae, Suchera Thananimit
Track 3-2: Food Science & Technology
No. Title Page
Antioxidants in Serbian functional foods 243-246
T3-IF1
Authors: Dubravka Štajner*, Boris M. Popović, Jasna Čanadanović-Brunet, Nataša Milić
Feasibility of utilizing skimmilk powder and whey protein powder in Greek-style 247-250 yogurt production T3-OF6
Authors: Chanokphat Phadungath*
Development of seaweed sorbet using Kappaphycus alvarezii 251-255 T3-OF7
Authors: Liyana Mohammed*, Sharifudin Md Shaarani
Nutritional composition, antioxidant activity and total phenolic content of bread 256-262 incorporated with seaweed (Sargassum Polycystum sp.) T3-OF8
Authors: Haini N*, Matanjun P, Song T.T, Yasmin O.B.H
Histopathological alterations and antioxidant enzymes activities in organs of 263-269 T3-OF9 hyperlipidaemic rats fed red seaweed, Gracilaria changii powder
Authors: Pei Teng Chan*, Patricia Matanjun, Suhaimi Md Yasir, Tek Song Tan
Pomergranate seed oil encapsulation in the zein nanoparticles 270-273 T3-OF10
Authors: Hamid Tavakolipour*, Ashkan Madadlou, Mohsen Mokhtarian
Track 3-2: Food Science & Technology
No. Title Page
Characterization of digestive enzyme extracted from Nile tilapia (Oreochromis 274-277 T3-PF7 niloticus) Viscera
Authors: Tatchaporn Chaijaroen*, Chakree Thongraung
Effect of sodium alginate coating on product characteristics, water loss, solid gain, 278-281 T3-PF8 ascorbic acid degradation during the osmotic dehydration of guava
Authors: Opasinee Kongmaneepitak, Saowanee Lertworasirikul*
Effect of sorbitol stress on polyamine content and agmatinase activity in wild type 282-285 T3-PF9 and ∆speB2 mutant of Synechocystis sp. PCC 6803
Authors: Supapid Eknikom, Aran Incharoensakdi, Saowarath Jantaro*
Characterization of GABA-producing probiotic isolated from Thai fermented foods 286-289 T3-PF10
Authors: Nantana Yuthaworawit*, Chalat Santivarangkna*
Polyhydroxybutyrate (PHB) content and its biosynthetic gene expression in 290-293 cyanobacterium Synechosystis sp. PCC 6803 under mineral nutrient conditions T3-PF11
Authors: Suthira Utharn, Aran Incharoensakdi, Saowarath Jantaro*
Molecular characterization of estrogen receptor gene in the black tiger shrimp 294-298 T3-PF12 (Penaeus monodon)
Authors: Sripapan Tharanat, Natthaporn Khwanmuang, Rachanimuk Hiransuchalert* Track 4: Bioactive natural products
No. Title Page
Native plants in Serbia-Opportunity for new herbal drugs 300-304 PL-2
Authors: Neda Mimica- Dukić*, Ivana Beara, Marija Lesjak, Emilija Jovin, Nataša Simin
Protective role of N-trans-feruloyltyramine in hydrogen peroxide-induced cell death 305-309 T4-O2
Authors: Ei Ei Phyo Myint, Rungtip Soi-ampornkul, Wanphen Katanyoo, Wipawan Thangnipon, Patoomratana Tuchinda, Saksit Nobsathian, Chalermchai Mitrpant*
Extraction and determination the effectivess of teh-tehan leaf (Acalypha 310-312
siamensis) against Staphylococcus aureus bacteria
T4-O3
Authors: Anggraeni Wulandari*, Nikmahtul Eviana, Reni Desiriana*, Arniz Hanifa, Munji Lestari
Anti-HIV proteins from two varieties of Momordica charantia cultivated in Thailand 313-314
T4-O5
Authors: Wichet Leelamanit*, Thitidaj Luetrakull
Setting time, surface pH and fluoride release of novel chitosan modified glass- 315-318 ionomer cement T4-P3
Authors: Sareepah Manmoo*, Ureporn Kedjarune-Leggat , Chanothai Hengtrakool
Effects of Musa sapientum extract on biofilm formation and acidogenicity of 319-322 T4-P4 Streptococcus mutans
Authors: Uraiwan Sungchum*, Sissada Tannukit, Suwanna Jitpukdeebodintra
Antimicrobial activities of Zingiberaceae extracts against fungi producing aflatoxin 323-327 T4-P5 isolated from stored rice seeds and grains of Khoa Dawk Mali 105
Authors: Puntip Tan-a-ram*, Pituk Maolee, Anusara Janklang, Anuchida Chokmongkon, Oratai Faina
Track 4: Bioactive natural products
No. Title Page
Effect of degree of hydrolysis on the antioxidant activities of Skipjack tuna skin 328-332 T4-P6
Authors: Worrapanit Chansuwan, Pavinee Chinachoti*
-Glucosidase inhibitors from Java tea Orthosiphon aristatus 333-337 T4-P7 (Blume) Miq.
Authors: Doungkamon Toumsuk, Preecha Phuwapraisirisan*
Anti-HIV-1 integrase activity of Albizia procera (Roxb.) Benth bark 338-339 T4-P8
Authors: Pattarapan Panthong , Chatchai Wattanapiromsakul, Supinya Tewtrakul*
Antioxidant and antimicrobial activities of Delonix regia (Bojer) Raf. Extracts 340-344 T4-P9
Authors: Supakorn Boonyuen*, Niramol Sakayawong, Pariya Na Nakorn, Thanatphat phetcharat, Kamonthip Mungkalasawat
Biological activities of Aquilaria crassna leaf extracts 345-348 T4-P10
Authors: Tipakorn Mungprom*, Kanokporn Boonsirichai, Wichet Leelamanit
Biological activities of Momordica cochinchinensis oil seed extract 349-352 T4-P11
Authors: Nhunpong Daechacupt*, Wichet Leelamanit
Effects of polymer coating on drug granules for controlling drug delivery systems 353-357 T4-P12
Authors: Boonyong Punantapong*, Sudarath Suntaropas
A glucose/mannose-specific lectin with alpha-glucosidase inhibitory activity from 358-362 T4-P13 Sterculia monosperma Vent seeds
Authors: Aphichart Karnchanatat*, Polkit Sangvanich
Effects of ethanolic extracts of some plants of Zingiberaceae and Lamiaceae on 363-365 T4-P14 the growth of pathogenic and probiotic bacteria
Authors: Monton Visutthi*
Metabolite profiles of two cultivars of Thai rice Oryza sativa L. that are different in 366-369 salt tolerance T4-P15
Authors: Kwankao Karnpakdee*
Screening of potential anti-fungal enzymatic activity in elicited callus culture of 370-374 T4-P16 Drumstrick tree (Moringa oleifera Lam.)
Authors: Wanutchaya Duanginta, Srisulak Dheeranupattana, Nopakarn Chandet, Lalida Shank* Track 5: Bio-Sensing Technology and Biomaterials
No. Title Page
Raman spectroscopy: A new alternative tool for food safety 376-379 T5-O1 Authors: Philippe Daniel*, Fabienne Lagarde-Abribat, Jean-François Pilard, Gérald Thouand,
Pranee Phinyocheep
Preliminary results of texture analysis in breast cancer microscopic image 380-383 T5-O2
Authors: Sirinapa Jitaree, Pornchai Phukpattaranont*
Graphene/polyaniline nanocomposite modified electrode for biosensors 384-387 * T5-P4
Authors: Jutiporn Yukird, Ratthapol Rangkupan, Trairak Pisitkun, Nadnudda Rodthongkum , Orawon
Chailapakul*Track 5: Bio-Sensing Technology and Biomaterials
No. Title Page
Colorimetric detection of trinitrotoluene via glutathione-capped silver nanaporticles 388-392 T5-P5
Authors: Pichaya Wilaivarangkul, Panote Thavarungkul, Proespichaya Kanatharana, Warakorn Limbut*
Cryogel entrapped acetylacetone sol-gel sensor for formalin detection 393-396 T5-P6 Authors: Lapasrada Aksornneam, Proespichaya Kanatharana, Panote Thavarungkul,
Chongdee Thammakhet*
397-400 Mixed-mode cation exchange for the extraction of -agonists
T5-P7
Authors: Supattri Noosang, Opas Bunkoed, Panote Thavarungkul, Proespichaya Kanatharana*
Electrochemical detection of trinitrotoluene using a poly(1,3,5-triazine-2,4,6- 401-404 triamine) modified glassy carbon electrode T5-P8
Authors: Suparat Cotchim, Sirinun Kanjun, Panote Thavarungkul, Proespichaya Kanatharana, Warakorn Limbut*
Bismuth-modified poly(glutamic acid)/glassy carbon electrode for zinc detection by 405-409 anodic stripping voltammetry T5-P9
Authors: Anisah Dueraning, Proespichaya Kanatharana, Panote Thavarungkul, Warakorn Limbut*
Electrochemical sensor for carbaryl detection based on ordered mesoporous 410-414 carbon modified electrode T5-P10
Authors: Jiranan Sangduangyang, Panote Thavarungkul, Proespichaya Kanatharana, Apon Numnuam*
Silver nanodendrite/carbon nanotubes modified electrode for hydrogen peroxide 415-419 T5-P11 detection
Authors: Jittima Choosang, Proespichaya Kanatharana, Panote Thavarungkul, Apon Numnuam*
Extracted substances of mangosteen pericarps from methanol extraction and their 420-423 T5-P12 monomer types analyzed from UPLC/MS
Authors: Narita Khundamri, Chahinez Aouf, H élène Fulcrand, Eric Dubreucq, Varaporn Tanrattanakul*
Synthesis of waterborne polyurethane containing natural rubber and poly(ethylene 424-427 glycol) T5-P13
Authors: Kanokporn Puttaro*, Chuanpit Khaokong
Track 1: Plant Animal and Environmental Science
The use of Mangrove Crab (Scylla sp.) shell chitosan as heavy metal lead
(Pb) adsorbent
a, a aIlma Fistannisa Zette * , Andhika Puspito Nugroho
Faculty of Biology, University Gadjah Meda, Jalan Teknika Selatan, Sekip Utara, Yogyakarta, Indonesia 55281
*Corresponding author. Tel: +6281215521422. E-mail address: [email protected]Abstract. Heavy metal pollution of lead (Pb) in the environment can be addressed by lowering the concentration of Pb using
adsorption techniques. Chitosan is a biopolymer chitin deacetylation modifies it into a potential heavy metal adsorbent. One source is the chitosan shell from mud crab (Scylla sp.) common as household eating waste. This study aims to determine the efficiency of the use of mud crab shells as raw material for chitosan and to determine the most optimal chitosan concentration and stirring time for the adsorption of Pb. Isolation of chitin done with deproteination and demineralization of mud crab shell powder. Chitosan is obtained from chitin deacetylation. Adsorption by chitosan performed on 27 samples of 1 ppm Pb 10 ml with chitosan concentration variation of 0.1;0.3;0.5 grams and stirring time for 5;10;15 minutes. Determination of heavy metal content in the samples was done using Atomic Absorption Spectrophotometer (AAS). The results showed that the efficiency of the mangrove crab shell chitosan as raw materials amounted to 29.67%. The most optimal chitosan concentration and stirring time for 1 ppm Pb adsorption is 0.5 grams chitosan with 15 minutes stirring time.
Keywords: chitosan; lead; Scylla sp.
Heavy metal pollution especially in water is very harmful to organisms. Heavy metals have toxic effects that influence biological processes and even can cause death. This is because heavy metals are not able to be broken down through biological processes and tend to settle to the bottom of waters associated with organic compounds in the sediment. Various cases of heavy metal pollution affects humans. One of the first known cases of the world is the death of cats and crows in Minamata Bay area, Japan and unexplainable disease among some residents of surrounding Jintsu River. They suffered from mercury poisoning from the Chisso factory wastewater (Iyoda, 2010). Heavy metals into the aquatic environment is usually derived from industrial waste. Concentration increases due to continuous discharge unchecked will degrade water quality (Banerjee, 2002). One of the heavy metals is lead (Pb) which is one of the most dangerous heavy metals for human health (Scoullos et al., 2001).
Lead is a heavy metal that is bluish-gray. Lead is present in nature in rocks, soil, and vegetation. While lead is released as a result from mining, smelting, refining, and secondary reprocessing (Anies, 2006). Lead is used as a material for producing products such as ammunition, wire coatings, pipes and solder, chemicals, dyes, and others. However, lead is most widely used to manufacture batteries. In addition in the form of particles, lead also we often encounter in the form of gas (metal fumes). Gases that pollute the air with lead is largely tetraethyl and tetramethyl- Pb-Pb derived from the combustion of motor vehicle gasoline additives (Fardiaz, 1992).
To overcome Pb pollution caused by industry requires an effort. Efforts are made to reduce the concentration of pollutants to below the threshold of environmental standards before being discharged into the environment. The method that is themost inexpensive and practical is an adsorption technique using a low-cost adsorbent such as seafood processing waste (Umaningrum et al., 2010). Waste crab shell waste is one that can be used as an adsorbent for heavy metal Pb. Chitin can be converted to chitosan by a deacetylation process. Chitosan is able to absorb heavy metal ions cations effectively because it has a hydroxyl group and an amino along the polymer chain (Sanjaya and Yuanita, 2007).
Chitosan is a biopolymer produced from deacetylation of chitin. Chitosan structure is composed of glucosamine and N-acetylglucosamine. Chitosan is also called poly (1,4)-2-amine-2-deoxy- β-D-glucose (Kurniasih and Kartika,
2011). Chitosan is widely used in various industries and is made from seafood industry waste which is available in large quantities and is a non-toxic and biodegradable (Suhardi, 1992). Chitosan is an abundant source material. Chitosan has been used extensively with a wide range of utilization. Chitosan is utilized as a a food preservative, in slimming drugs, cosmetics, as an anti-microbial, and in sewage treatment, etc. (Mahatmanti et al., 2010).
In the manufacture of chitosan, there are three main processes deproteinisation, demineralization, and deacetylation. Deproteinisation and demineralization is achieved by using a solution of acids and bases, to remove proteins and minerals in order to obtain purified chitin. Chitin is then converted into chitosan by deacetylation by heating in an alkaline solution (Rege and Lawrence, 1999; Tolaimatea, et al., 2003)
Mangrove crabs are crabs that live in coastal waters, particularly in the mangrove forests (mangroves). Indonesia is known as a large exporter of crabs. Mangrove crab (Scylla sp.) is one of Indonesia’s fishery commodities that is
in demand both at home and abroad. Consumption is high due to their delicious taste and are highly nutritious. However, the waste generated from the consumption of shell mud crab cannot be used optimally. Mangrove crab shells contain chitin and are readily available, therefore we have studied the adsorption of chitosan from mangrove crab shells against one particularly dangerous heavy metal, namely lead (Pb).
Based on information from a variety of references, the efficiency of mud crab shells as a source of chitosan is 50- 60%. In addition, the concentration and the duration of stirring in the adsorption stage metal lead (Pb) by chitosan will affect a decrease in metal content in the solution, ie the higher the concentration and stirring time, the adsorption of Pb will be even greater. This study aims to determine the efficiency of mud crab shells as raw material for chitosan and to determine the most optimum chitosan concentration and stirring time for the adsorption of Pb.
2. Materials and Methods
The mud crab shells were collected from a local market in Pati, Central Java, Indonesia. The viscera and tissues were carefully removed. The shells were washed and dried with no sun light. The sample was then deproteinized with 3% NaOH at a ratio of 1:6. The mixture was heated to 80-85 °C for 30 min. After it was filtered, the residue was washed with distilled water until no longer alkaline. The powder was dried in an oven at a temperature of 70-80 °C for 24 hours. Crab shell powder that has undergone deproteinisation was then demineralized at 1.25 N HCl in the ratio 1:10. This mixture is stirred using a magnetic stirrer for 2 hours. The residue was heated in a temperature of 70-75 °C for 1 hour. The resulting product ischitin in the form of a pale cream-colored powder. Chitin was deacetylated with 50% NaOH at a ratio of 1:10 and then heated at a temperature of 95-100 °C for 30 min. Chitosan powder obtained was washed with distilled water until neutral, then filtered and dried in an oven at a temperature of 80 °C for 24 hours. The chitosan yield was calculated by comparing the weight measurements of the raw material to the chitosan obtained after treatment. Adsorption by chitosan performed on 27 samples of 1 ppm Pb 10 ml with chitosan concentration variation of 0.1;0.3;0.5 grams and stirring time for 5;10;15 minutes. The results were quantitatively and qualitatively analyzed. Quantitative comparison of the data was Pb concentration of the control and treatment group that analyzed using Atomic Absorption Spectrophotometry. The qualitative data were analyzed by one way ANOVA and Tukey HSD test.
3. Results and Discussion
Pb concentration in each treatment was significance tested and the results indicated that there was significant difference between each treatment. The data presented in the graph (fig.1) shows the correlation between time stirring with a decrease in the concentration of Pb. In addition to the effect of different chitosan concentrations the decrease in the concentration of Pb is also different.
Fig. 1. Influence of stirring time and chitosan concentration on the residual concentrations of Pb. Figure 1 shows the effect of chitosan on the Pb concentration. Control treatment did not alter the concentration of 1 ppm Pb treatment while using chitosan is able to decrease the concentration of chitosan. Sequentially the biggest drop was in 0.5 g chitosan followed by 0.3 grams and 0.1 grams. In each chitosan concentration decreased Pb concentration and binding increased over stirring time, so that the most significant time decrease the concentration of Pb is 15 minutes. Overall, the most optimal treatment lowers the concentration of Pb is chitosan concentration of 0.5 g for 15 min stirring time, amounting to 93.6% completion of absorption
Table 1. The efficiency of mud crab shell as raw material of chitosan.
Weight of mud crab shell Weight of chitosan yield Efficiency
powder 30 gram 8,9 gram 29,67% The use of mud crab shells as raw material chitosan is an effort in the utilization of the waste feed of crab shells.From this study the efficiency of the mangrove crab shell chitosan as raw material for absorption is relatively low (29.67%) (Table1). The accuracy of the method can affect the weight of the resulting chitosan. Deacetylation reaction of chitin into chitosan is influenced by many factors that most chitin may not be perfectly transformed into chitosan.
The ability of chitosan as an adsorbent of heavy metals, particularly Pb characterized by a decrease in the concentration of Pb in solution. In this study 1 ppm Pb concentration decreased with the addition of chitosan (Figure 1). The higher the concentration of chitosan used, the greater the reduction in Pb concentration. The largest concentration of chitosan, which resulted in a decrease of 0.5 grams of the greatest concentrations of Pb. While the smallest chitosan concentration (0.1 grams), resulted in the lowest decrease in the concentration of Pb smallest. Chitosan has electrons of nitrogen in the amine group capable of covalent bonds with transition metal ions. As an electron donor, the concentration of chitosan affects the number of electrons available. The more electrons are available, the more covalent bonds are formed with metal ions. The bond formed in chitosan-metal complexes that can reduce the effects of environmental pollutants such as Pb (Suharsih, 2008).
In an adsorption process, influencing factors, among others, the stirring speed and contact time. Stirring speed will determine the speed of the adsorption process, while the contact time can produce the maximum adsorption capacity (Syauqiah et al., 2011). The longer the contact time, the greater the adsorption. From this research it has been found that the contact time of 15 minutes is an optimal time in Pb heavy metal adsorption by chitosan (Figure 1). It is not known whether this time is a time of equilibrium that produces the maximum adsorption capacity. Thus need to conduct further research with a wider range of time.
4. Conclusions
The efficiency of the mangrove crab shell as raw materials of chitosan amounted to a yield of 29.67 %. The greater the concentration of chitosan and long stirring time, the greater the concentration of Pb will decrease .The most optimal chitosan concentration and stirring time for 1 ppm Pb adsorption is 0.5 grams chitosan with 15 minutes stirring time.
5. Acknowledgements
The authors gratefully acknowledge the contributions of Faculty of Biology UGM and LPPT UGM for supporting the research.
6. Conflict of interest Conflict of interest: none.
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