Hubungan Ekspresi Imunohistokimia Vascular Endothelial Growth Factor (VEGF) dan Tumor-infiltrating lymphocytes (TILs) dengan Tipe Histopatologi dan Stadium Klinis Karsinoma Nasofaring

LAMPIRAN 1
MASTER DATA

No.

No. PA

Usia/

Stadium

Diagnosa

TILs

IHK

JK

Klinis

1. B/319/12

59/L

Dini

KSCC

High

Negatif

2. B/336/12

36/L

Dini

NKSCC (Differentiated subtype)

High

Ekspresi kuat

3. B/572/12

57/L

Lanjut

NKSCC (Differentiated subtype)

High

Ekspresi lemah

4. B/1074/12

55/L

Dini

NKSCC (Differentiated subtype)

Intermediate

Negatif

5. B/2185/12

53/P

Dini

KSCC

High

Ekspresi lemah

6. B/3170/12

24/P

Lanjut

KSCC

Low

Ekspresi kuat

7.

B/2450/13

51/L

Lanjut

NKSCC (Differentiated subtype)

Intermediate

Negatif

8. B/3063/13

47/L

Lanjut

NKSCC (Undifferentiated subtype)

High

Ekspresi kuat

9. B/3672/13

69/L

Dini

NKSCC (Undifferentiated subtype)

Intermediate

Ekspresi lemah

10. B/3863/13

41/P

Dini

NKSCC (Differentiated subtype)

Low

Negatif

11. B/3976/13

49/L

Lanjut

NKSCC (Undifferentiated subtype)

Intermediate

Ekspresi lemah

12. B/4269/13

59/L

Lanjut

NKSCC (Undifferentiated subtype)

Intermediate

Ekspresi lemah

13. B/4307/13

50/P

Lanjut

NKSCC (Undifferentiated subtype)

High

Negatif

14. B/4426/13

59/L

Dini

NKSCC (Differentiated subtype)

High

Ekspresi lemah

15. B/4456/13

36/P

Lanjut

NKSCC (Differentiated subtype)

Intermediate

Negatif

16. B/4500/13

60/L

Lanjut

NKSCC (Undifferentiated subtype)

Intermediate

Negatif

17. B/4776/13

18/L

Lanjut

NKSCC (Undifferentiated subtype)

Intermediate

Negatif

18. B/4816/13

36/P

Lanjut

NKSCC (Differentiated subtype)

High

Ekspresi lemah

19. B/4925/13

68/P

Dini

NKSCC (Undifferentiated subtype)

Intermediate

Negatif

20. B/4927/13

56/P

Lanjut

NKSCC (Differentiated subtype)

Intermediate

Negatif

21. B/5027/13

37/L

Lanjut

NKSCC (Undifferentiated subtype)

High

Negatif

22. B/5077/13

27/L

Lanjut

NKSCC (Differentiated subtype)

Intermediate

Ekspresi kuat

23. B/5079/13

33/L

Lanjut

NKSCC (Undifferentiated subtype)

High

Negatif

24. B/5143/13

50/L

Lanjut

NKSCC (Undifferentiated subtype)

Low

Negatif

25. B/5145/13

51/L

Dini

NKSCC (Differentiated subtype)

High

Negatif

26. B/5216/13

29/L

Lanjut

NKSCC (Differentiated subtype)

Low

Ekspresi kuat

VEGF

Universitas Sumatera Utara

27. B/5218/13

42/L

Lanjut

NKSCC (Undifferentiated subtype)

High

Negatif

28. B/5443/13

65/P

Lanjut

NKSCC (Undifferentiated subtype)

Intermediate

Ekspresi lemah

29. B/5542/13

41/P

Dini

NKSCC (Undifferentiated subtype)

Intermediate

Ekspresi lemah

30. B/5687/13

46/L

Dini

NKSCC (Undifferentiated subtype)

Low

Negatif

31. B/5764/13

66/L

Dini

NKSCC (Differentiated subtype)

High

Negatif

32. B/5990/13

48/P

Lanjut

NKSCC (Differentiated subtype)

Intermediate

Ekspresi lemah

33. B/5995/13

59/P

Lanjut

NKSCC (Undifferentiated subtype)

High

Ekspresi lemah

34. B/6100/13

63/P

Lanjut

NKSCC (Undifferentiated subtype)

Low

Ekspresi lemah

35. B/6786/13

48/P

Lanjut

NKSCC (Undifferentiated subtype)

Low

Ekspresi lemah

36. B/6823/13

73/P

Lanjut

NKSCC (Undifferentiated subtype)

High

Ekspresi kuat

37. B/7161/13

50/L

Lanjut

NKSCC (Undifferentiated subtype)

High

Ekspresi lemah

38. B/7275/13

36/L

Lanjut

NKSCC (Undifferentiated subtype)

High

Negatif

39. B/1100/13

44/L

Dini

NKSCC (Differentiated subtype)

Low

Ekspresi lemah

40. B/1598/13

54/L

Lanjut

NKSCC (Undifferentiated subtype)

High

Ekspresi lemah

41. B/1529/13

46/L

Lanjut

NKSCC (Undifferentiated subtype)

Low

Ekspresi lemah

42. B/4425/13

58/L

Dini

NKSCC (Differentiated subtype)

High

Ekspresi kuat

Universitas Sumatera Utara

LAMPIRAN 2. ANALISA STATISTIK

-> tabulation of sex
sex

Freq.

Percent

Cum.

laki
perempuan

27
15

64.29
35.71

64.29
100.00

Total

42

100.00

std

Freq.

Percent

Cum.

awal
lanjut

14
28

33.33
66.67

33.33
100.00

Total

42

100.00

-> tabulation of std

-> tabulation of grade
grade

Freq.

Percent

Cum.

KSCC
NKSCC diff
NKSCC undiff

3
16
23

7.14
38.10
54.76

7.14
45.24
100.00

Total

42

100.00

TILs

Freq.

Percent

Cum.

low
Intermediate
high

9
14
19

21.43
33.33
45.24

21.43
54.76
100.00

Total

42

100.00

-> tabulation of tils

-> tabulation of vegf
VEGF

Freq.

Percent

Cum.

neg
low
over

18
16
8

42.86
38.10
19.05

42.86
80.95
100.00

Total

42

100.00

Universitas Sumatera Utara

. sum umur
Variable

Obs

Mean

umur

42

48.78571

Std. Dev.

Min

Max

12.73661

18

73

. tab vegf grade, row

Key
frequency
row percentage

VEGF

KSCC

grade
NKSCC dif

NKSCC und

Total

neg

1
5.56

7
38.89

10
55.56

18
100.00

low

1
6.25

5
31.25

10
62.50

16
100.00

over

1
12.50

4
50.00

3
37.50

8
100.00

Total

3
7.14

16
38.10

23
54.76

42
100.00

. tab vegf std, row

Key
frequency
row percentage

VEGF

std
awal

lanjut

Total

neg

7
38.89

11
61.11

18
100.00

low

5
31.25

11
68.75

16
100.00

over

2
25.00

6
75.00

8
100.00

Total

14
33.33

28
66.67

42
100.00

Universitas Sumatera Utara

. tab grade

tils, row

Key
frequency
row percentage

grade

low

TILs
Intermedi

high

Total

KSCC

1
33.33

0
0.00

2
66.67

3
100.00

NKSCC diff

3
18.75

6
37.50

7
43.75

16
100.00

NKSCC undiff

5
21.74

8
34.78

10
43.48

23
100.00

Total

9
21.43

14
33.33

19
45.24

42
100.00

. tab tils std, row

Key
frequency
row percentage

TILs

std
awal

lanjut

Total

low

3
33.33

6
66.67

9
100.00

Intermediate

4
28.57

10
71.43

14
100.00

high

7
36.84

12
63.16

19
100.00

Total

14
33.33

28
66.67

42
100.00

Universitas Sumatera Utara

. kwallis grade, by (tils)
Kruskal-Wallis equality-of-populations rank test

tils

Obs

Rank Sum

low
Intermediate
high

9
14
19

191.50
317.00
394.50

chi-squared =
probability =

0.193 with 2 d.f.
0.9080

chi-squared with ties =
probability =
0.8837

0.247 with 2 d.f.

. kwallis std , by (tils)
Kruskal-Wallis equality-of-populations rank test

tils

Obs

Rank Sum

low
Intermediate
high

9
14
19

193.50
315.00
394.50

chi-squared =
probability =

0.162 with 2 d.f.
0.9224

chi-squared with ties =
probability =
0.8859

0.242 with 2 d.f.

Universitas Sumatera Utara

. kwallis std , by (vegf)
Kruskal-Wallis equality-of-populations rank test

vegf

Obs

Rank Sum

neg
low
over

18
16
8

366.00
351.00
186.00

chi-squared =
probability =

0.346 with 2 d.f.
0.8412

chi-squared with ties =
probability =
0.7716

0.519 with 2 d.f.

. kwallis grade, by (vegf)
Kruskal-Wallis equality-of-populations rank test

vegf

Obs

Rank Sum

neg
low
over

18
16
8

392.50
369.50
141.00

chi-squared =
probability =

1.079 with 2 d.f.
0.5829

chi-squared with ties =
probability =
0.5009

1.383 with 2 d.f.

.

Universitas Sumatera Utara

LAMPIRAN 3

Universitas Sumatera Utara

LAMPIRAN 4

Universitas Sumatera Utara

LAMPIRAN 5

Universitas Sumatera Utara

LAMPIRAN 6
SURAT TANDA BUKTI
TELAH DILAKUKAN PEMBACAAN ULANG SLAID

Judul Penelitian

: Hubungan Ekspresi Imunohistokimia
Vascular Endothelial Growth Factor (VEGF) dan
Tumor-infiltrating lymphocytes (TILs) dengan
Tipe Histopatologi dan Stadium Klinis
Karsinoma Nasofaring

Nama

: Nancy Sartika Tambunan

NIM

: 107108003

Program Studi

: Program Pendidikan Dokter Spesialis Patologi Anatomik
Fakultas Kedokteran Universitas Sumatera Utara

Telah dilakukan pembacaan ulang slaid pada :
Hari/Tanggal

: Senin, 17 Oktober 2016 dan Selasa, 18 Oktober 2016

Didampingi oleh
Pembimbing

: 1. dr. H. Soekimin, Sp.PA(K)
2. dr. H. Joko S. Lukito, Sp.PA(K)

Medan, 20 Oktober 2016
Pembimbing I

dr. H. Soekimin, Sp.PA(K)

Pembimbing II

dr. H. Joko S. Lukito, Sp.PA(K)

Universitas Sumatera Utara

LAMPIRAN 7. GAMBARAN MIKROSKOPIS EKSPRESI VEGF

Karsinoma nasofaring
VEGF negatif (100x)

Karsinoma nasofaring
VEGF negatif (400x)

Karsinoma nasofaring
VEGF ekspresi lemah (100x)

Karsinoma nasofaring
VEGF ekspresi lemah (400x)

Karsinoma nasofaring
VEGF ekspresi kuat (100x)

Karsinoma nasofaring
VEGF ekspresi kuat (400x)
Universitas Sumatera Utara

GAMBARAN MIKROSKOPIS TILs

Karsinoma nasofaring
Low TILs (HE 200x)

Karsinoma nasofaring
Low TILs (HE 400x)

Karsinoma nasofaring
Intermediate TILs (HE 200x)

Karsinoma nasofaring
Intermediate TILs (HE 400x)

Karsinoma nasofaring
High TILs (HE 200x)

Karsinoma nasofaring
High TILs (HE 400x)
Universitas Sumatera Utara