Hubungan Ekspresi Imunohistokimia Vascular Endothelial Growth Factor (VEGF) dan Tumor-infiltrating lymphocytes (TILs) dengan Tipe Histopatologi dan Stadium Klinis Karsinoma Nasofaring

ABSTRAK
HUBUNGAN EKSPRESI IMUNOHISTOKIMIA
VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) DAN
TUMOR-INFILTRATING LYMPHOCYTES (TILs) DENGAN TIPE
HISTOPATOLOGI DAN STADIUM KLINIS KARSINOMA NASOFARING
Nancy Sartika Tambunan1, Soekimin1, Joko S. Lukito1
1

Departemen Patologi Anatomik Fakultas Kedokteran USU

Latar belakang. Karsinoma nasofaring merupakan karsinoma yang berasal dari mukosa
nasofaring yang secara mikroskopis tampak berupa diferensiasi skuamosa. Beberapa target
molekuler telah diidentifikasi dalam spesimen tumor penderita karsinoma nasofaring. VEGF
merupakan faktor proangiogenik, yang berperan dalam angiogenesis untuk pertumbuhan
tumor, invasi, dan metastasis tumor. Limfosit T dikenal sebagai main effector dari respon
imun anti tumor, yang sering dihubungkan dengan gambaran klinis yang lebih baik. Penderita
karsinoma nasofaring dapat diklasifikasikan berdasarkan stadium klinis dengan
menggunakan sistem TNM.
Tujuan. Untuk mengetahui hubungan ekspresi imunohistokimia VEGF dan Tumorinfiltrating lymphocytes (TILs) dengan tipe histopatologi dan stadium klinis karsinoma
nasofaring.
Bahan dan Metode. Penelitian ini merupakan penelitian deskriptif analitik dengan

pendekatan cross sectional. Sampel pada penelitian ini adalah slaid dan blok parafin dari
jaringan nasofaring yang telah didagnosa sebagai karsinoma nasofaring. Blok parafin tersebut
kemudian dipulas dengan Hematoxilin-Eosin dan imunohistokimia VEGF. Ekepresinya akan
dianalisa dengan Kruskal-Wallis untuk melihat hubungannya dengan tipe histopatologi dan
stadium klinis karsinoma nasofaring.
Hasil. Berdasarkan analisa statistik Kruskal-Wallis terhadap 42 sampel kasus karsinoma
nasofaring menguji hubungan ekspresi imunohistokimia VEGF dengan tipe histopatologi
karsinoma nasofaring, diperoleh p-value = 0,501 (p>0,05), hubungan ekspresi
imunohistokimia VEGF dengan stadium klinis karsinoma nasofaring, diperoleh p-value =
0,772 (p>0,05), hubungan TILs dengan tipe histopatologi karsinoma nasofaring, diperoleh pvalue = 0,884 (p>0,05) dan hubungan TILs dengan stadium klinis karsinoma nasofaring,
diperoleh p-value = 0,886 (p>0,05).
Simpulan. Tidak ada hubungan yang bermakna antara ekspresi imunohistokimia VEGF dan
TILs dengan tipe histopatologi dan stadium klinis karsinoma nasofaring.
Kata kunci : karsinoma nasofaring, VEGF, TILs, tipe histopatologi, stadium klinis

Universitas Sumatera Utara

ABSTRACT
CORRELATION BETWEEN THE EXPRESSION OF
IMMUNOHISTOCHEMISTRY VASCULAR ENDOTHELIAL GROWTH

FACTOR (VEGF) AND TUMOR-INFILTRATING LYMPHOCYTES (TILs)
WITH HISTOPATHOLOGICAL TYPE AND CLINICAL STAGING OF
NASOPHARYNGEAL CARCINOMA
Nancy Sartika Tambunan1, Soekimin1, Joko S. Lukito1
Department of Anatomical Pathology, Faculty of Medicine, USU

1

Background. Nasopharyngeal carcinoma is a carcinoma arising in the nasopharyngeal
mucosa that shows light microscopic of squamous differentiation. Several molecular targets
have been identified in tumour specimen of patients with nasopharyngeal carcinoma. VEGF
is an angiogenesis factor, which contributes to angiogenesis and subsequent growth, invasion
and metastasis of tumours. T lymphocytes are recognized as the main effectors of anti tumor
immune responses. They often associated with better clinical outcomes. Nasopharyngeal
carcinoma patients can be classified according to their clinical stage in the system. The
system that we used are TNM stage, T-stage and metastasis of nasopharyngeal carcinoma.
Objective. To investigate correlation between the expression of immunohistochemistry
Vascular Endothelial Growth Factor (VEGF) and Tumor-infiltrating lymphocytes (TILs) with
histopathological type and clinical staging of nasopharyngeal carcinoma.
Material and Methods. This is an analytical descriptive study with cross sectional approach.

The sample of this study are slides and paraffin blocks of nasopharyngeal tissue which
diagnose histopatologically as nasopharyngeal carcinoma. Then the samples were stained
with Hematoxilin Eosin and VEGF. Their expressions will be analyzed with Kruskal-Wallis
statistical analysis to investigate the correlation with histopathological type and clinical
staging of nasopharyngeal carcinoma.
Results. Based on an analysis statistic Kruskal-Wallis test to 42 sample cases nasopharyngeal
carcinoma to evaluated the correlation between the expression of VEGF with
histopathological type with p-value = 0,501 (p>0,005), the correlation between the expression
of VEGF with clinical staging with p-value = 0,772 (p>0,005), the correlation between TILs
with histopathological type with p-value = 0,884 (p>0,005), the correlation between TILs
with clinical staging with p-value = 0,886 (p>0,005).

Conclution. There is no significant correlation between the expression of
immunohistochemistry VEGF and TILs with histopathological type and clinical staging of
nasopharyngeal carcinoma.
Keywords: nasopharyngeal carcinoma, VEGF, TILs, histopathological type, clinical staging

Universitas Sumatera Utara