a. Kuersetin
i. Konsentrasi 1µgml
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ii. Konsentrasi 2µgml
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iii. Konsentrasi 3µgml
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iv. Konsentrasi 4µgml
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v. Konsentrasi 5µgml
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b. Ekstrak etanolik biji C. moschata
i. Konsentrasi 25µgml
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ii. Konsentrasi 50µgml
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iii. Konsentrasi 75µgml
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iv. Konsentrasi 100µgml
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v. Konsentrasi 125µgml
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vi. Konsentrasi 150µgml
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4. Kurva Regresi Linier Hubungan Inhibisi dengan Seri Kadar
Ektrak Etanolik Biji C. moschata
a. Kuersetin
b. Ekstrak etanolik biji C. moschata
5. Perhitungan IC
50
a. Kuersetin
Aktivitas hambat antioksidan kuersetin mencapai 50 pada konsentrasi 2,55 µgml.
y = 18.192x + 3.578 R² = 0.9963
20 40
60 80
100
2 4
6
in h
ib isi
Konsentrasi µgml
kuersetin Linear kuersetin
y = 0.1113x + 3.2447 R² = 0.9904
5 10
15 20
25
50 100
150 200
i nh
ibis i
Konsentrasi µgml
ekstrak etanolik biji C. moschata
Linear ekstrak etanolik biji C. moschata
b. Ekstrak etanolik biji C. moschata
Maka aktivitas hambat antioksidan ekstrak etanolik biji C. moschata mencapai 50 pada konsentrasi 420,08 µgml.
Lampiran 5. Uji Aktivitas Antibakteri
Ekstrak etanolik biji C. moschata dilarutkan dalam DMSO
1. Hasil pengamatan DZI uji antimikroba pembanding tetrasiklin
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2. Hasil pengamatan DZI uji antimikroba pembanding ciprofloksasin
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Naskah Publikasi Karya Tulis Ilmiah
Ika Dewi Rahmawati 20120350024 Farmasi UMY
Page 1
Uji Aktivitas Antioksidan dan Antibakteri Ekstrak Etanolik Biji Labu Kuning
Cucurbita moschata Duch Poir Antioxidant and Antibacterial Activity of Pumpkin Seeds
Cucurbita moschata Duch Poir Ethanolic Extract
Sri Tasminatun, M.Si., Apt, Rifki Febriansah, M.Sc., Apt, Ika Dewi Rahmawati, Dr. Alfaina Wahyuni, Sp. OG
Pharmacy Study Program, Faculty of Medical and Health Sciences, Muhammadiyah University of Yogyakarta
ABSTRACT
Pumpkin Cucurbita moschata Duch Poir is one of the plants that are easily found in Indonesia, but has not been used optimally. C. moschata seeds are
known to contain many different active compounds such as flavonoid and alkaloid. Flavonoid and alkaloid known to give the effect of antibacterial and anti
radical. Free radicals is one of the main causes of the occurrence of degenerative diseases. The infection caused by bacteria may also aggravate a degenerative
disease complications. This research aims to identify and investigate the effect of flavonoid and alkaloid compounds of ethanolic extract seeds of C. moschata as
antioxidant and antibacteria.
The seeds of C. moschata were extracted using maceration method with ethanol 70. The extract of C. moschata seeds was identified flavonoid and
alkaloid compounds using thin layer chromatography methods. Then tested its antioxidant activity using DPPH method and the antibacteria was tested by disc
diffusion method. The data inhibition got on the antioxidant activity testing poured into a curve between the inhibition and the concentration to get the
equivalence of linear regression. The equivalence of linear regression was used to decide Inhibitory Concentration IC
50
. On antibacteria activity testing, it was done with diameter zone of inhibition measurement method toward
Staphylococcus aureus bacteria, and then compared with the standard value of antibacterial activity.
The results of the identification showed the extract ethanolic of C. moschata seeds contain of flavonoid and alkaloid compounds with Rf 0.87 and
0.56. Compared to standard quercetin and kuinin compounds that showed Rf 0.75 and 0.56. This study showed that extracts ethanolic of C. moschata seeds had
weak antioxidant activity with IC
50
values of 420.08 µgml and has antibacterial effects on concentration of 25 1.31 mm and 50 2.24 toward the S. aureus
bacteria.
Key words: antibacteria, antioxidant, Cucurbita moschata, Staphylococcus aureus.
