Efek Ekstrak Etanol Daun Bangun-Bangun (Plectranthus amboinicus (Lour.) Spreng) Terhadap Kadar Nitro Oxide Pada Tikus Jantan yang Diinduksi Doksorubisin
Lampiran 1. Bagan Kerja Penelitian
Dikeringkan dalam lemari pengering
Dihaluskan Ditimbang
A
Daun bangun-bangun segar
Berat kering 589 g
Serbuk simplisia 570 g
Maserat Ampas
Ekstrak kental 42,010 g
Gambaran makroskopik
Penetapan kadar abu total
Penetapan kadar abu tidak
larut dalam asam
Penetapan kadar air
Penetapan kadar sari larut dalam air
Dimaserasi menggunakan etanol 96 %
Dikentalkan menggunakan rotavapor Berat basah 1,83 kg
Skrining fitokimia ekstrak dan karakterisasi ekstrak
Skrining fitokimia
Diberikan pada hewan percobaan Ekstraksi 500 g Dibersihkan dari pengotor Dicuci sampai bersih, dititiskan ditimbang
Plasma darah Pemeriksaan kerusakan organ jantung Diukur kadar
nitrat dan nitrit Karakterisasi simplisia
(2)
(3)
(4)
Lampiran 4. Gambar tumbuhan daun bangun-bangun
(5)
Lampiran 5. Gambar simplisia dan serbuk simplisia daun bangun-bangun
Plectranthus amboinicus (Lour.) Spreng)
(6)
Lampiran 6. Gambaran Mikroskopik simplisia daun bangun-bangun
Keterangan :
a. Kristal kalsium oksalat bentuk prisma b. Rambut penutup multiselluler
c. Rambut kelenjar bentuk glandular d. Pembuluh kayu berbentuk spiral
(7)
(8)
(9)
Lampiran 8. Perhitungan Hasil Penetapan Kadar Air Serbuk Simplisia Daun Bangun-bangun
Kadar air = � �
� � � � %
1. Sampel 1
Berat sampel = 5,011 g
Volume air = 0,4 ml
Kadar air = ,
, � %
= 7,98% 2. Sampel 2
Berat sampel = 5,081 g
Volume air = 0,4 ml
Kadar air = ,
, � %
= 7,87% 3. Sampel 3
Berat sampel = 5,009 g
Volume air = 0,4 ml
Kadar air = ,
, � %
= 7,98%
Kadar air rata - rata = , % + , % + , %
(10)
Lampiran 9. Perhitungan Hasil Penetapan Kadar Sari Larut dalam Air Serbuk Simplisia Daun Bangun-bangun
Kadar sari larut dalam air = � �
� � � �
x
x
100%1. Kadar sari larut dalam air I
Berat cawan = 45,368 g
Berat cawan + berat sari = 48,666 g
Berat sampel = 5,014 g
Berat sari = 0,298 g
Kadar sari larut dalam air = ,
,
x
x
100% = 29,72%2. Kadar sari larut dalam air II
Berat cawan = 47,964 g
Berat cawan + berat sari = 48,259 g
Berat sampel = 5,006 g
Berat sari = 0,295 g
Kadar sari larut dalam air = ,
,
x
x
100% = 29,46%3. Kadar sari larut dalam air III
Berat cawan = 44,349 g
Berat cawan + berat sari = 44,640 g
Berat sampel = 5,003 g
Berat sari = 0,291 g
Kadar sari larut dalam air = ,
,
x
x
100% = 29,07%(11)
Lampiran 10. Perhitungan Hasil Penetapan Kadar Sari Larut dalam Etanol Serbuk Simplisia Daun Bangun-bangun
1.Kadar sari larut dalam etanol I
Berat Cawan = 45,183 g
Berat Cawan + Berat Sari = 45,268 g
Berat Sampel = 5,008 g
Berat sari = 0,085 g
Kadar sari larut dalam etanol = ,
,
x
x
100%=8,49%
2.Kadar sari larut dalam etanol II
Berat Cawan = 45,177 g
Berat Cawan + Berat Sari = 45,260 g
Berat Sampel = 5,003 g
Berat sari = 0,083 g
Kadar sari larut dalam etanol = .
,
x
x
100%=8,30%
3.Kadar sari larut dalam etanol III
Berat Cawan = 43,101g
Berat Cawan + Berat Sari = 43,183g
Berat Sampel = 5,005 g
Berat sari = 0.082 g
Kadar sari larut dalam etanol = ,
,
x
x
100% = 8,19%Kadar sari larut dalam etanol
=
e i
(12)
Lampiran 11. Perhitungan Hasil Penetapan Kadar Abu Total Serbuk Simplisia Daun Bangun-bangun
1. Sampel I
Berat simplisia = 2,0010 g
Berat abu = 0,0180 g
Kadar abu total = ,
,
x
100% = 0,89%2. Sampel II
Berat simplisia = 2,0013 g
Berat abu = 0,0179 g
Kadar abu total = ,
,
x
100%= 0,89%3. Sampel III
Berat simplisia = 2,0011 g
Berat abu = 0,0168 g
Kadar abu total = ,
,
x
100% = 0,84%Kadarabu total rata-rata = , %+ , %+ , %
= 0,87%
Kadar abu total
=
e(13)
Lampiran 12. Perhitungan Hasil Penetapan Kadar Abu Total Larut Asam Serbuk Simplisia Daun Bangun-bangun
1. Sampel I
Berat simplisia = 2,0010 g
Berat abu = 0,0300 g
Kadar abu tidak larut asam = ,
,
x
100%= 1,49% 2. Sampel II
Berat simplisia = 2,0013 g
Berat abu = 0,0088 g
Kadar abu tidak larut asam = ,
, X 100%
= 0,44% 3. Sampel III
Berat simplisia = 2,0011 g
Berat abu = 0,0090 g
Kadar abu tidak larut asam = ,
,
x
100%= 0,45%
, % + , % + . %
Kadar abu tidak larut dalam asam
=
e(14)
Lampiran 13. Contoh Perhitungan Volume Suspensi Ekstrak dan Larutan Doksorubisin yang Diberikan pada Hewan Percobaan
Berat badan hewan = 229,4 g
a. Suspensi EEDBB 5% = 5 g / 100 ml
= 50 mg / ml
Dosis EEDBB = 300 mg/ kkBB
Dosis yang diberikan = x mg kg
kg g g / 300 / 1000 4 , 229
= 68,82 mg
Volume yang diberikan = x ml
mg 1
50 82 , 68
= 1,4 ml
b. Larutan Doksorubisin = 10 mg / 5 ml
= 2 mg / ml
Dosis Doksorubisn = 10 mg / kg BB
Dosis yang diberikan = x mg kg
kg g g / 10 / 1000 4 , 229
= 2,294 mg
Volume yang diinjeksikan = x ml
mg mg 1 2 294 , 2
(15)
Lampiran 14. Kurva Panjang Gelombang Maksimum Natrium Nitrit Baku
(16)
(17)
Lampiran 17. Perhitungan Persamaan Regresi Kurva Kalibrasi Nitrit
X Y X2 Y2 XY
0,000 0,006 0,000 0,000000 0,000000
0,050 0,040 0,003 0,001600 0,002000
0,100 0,065 0,010 0,004225 0,006500
0,150 0,090 0,023 0,008100 0,013500
0,200 0,119 0,040 0,014161 0,023800
0,400 0,225 0,160 0,050625 0,090000
0,600 0,330 0,360 0,108900 0,198000
0,800 0,436 0,640 0,190096 0,348800
1,000 0,535 1,000 0,286225 0,535000
1,200 0,640 1,440 0,409600 0,768000
∑X = 4,500 ∑Y = 2,486 ∑X2
= 3,675 ∑Y
2 = 1,073532 ∑XY = 1,985600
X = 0,450 = 0,248
a =
X
X
n n Y X XY / / 2 2
= 10 / ) 5 , 4 ( 675 , 3 10 / ) 480 , 2 )( 5 , 4 ( 9856 , 1 2 = 65 , 1 866936 , 0 = 0,525416 b = Y - aX= 0,2486 – 0,525416 (0,45) = 0,012157
r =
X X n
Y
Y n
n Y XY XY / / / ) )( ( 2 2 2
2
(18)
Lampiran 18. Contoh Perhitungan dan Tabel Kadar Nitrit dalam Plasma Pada kelompok blanko diperoleh absorbansi (Y) = 0,048
Persamaan regresi : Y = 0,525416X +0,012157 X = 525416 , 0 012157 , 0 Y X = 525416 , 0 012157 , 0 048 . 0 X= 0,068218
Faktor pengenceran = 50
Perhitungan kadar = X (konsentrasi) x Faktor pengenceran = 0,068218 x 50
= 3,4109 µg/ml
Kelompok Absorbansi (Y) Konsentrasi (X) Kadar (µ g/ml)
Blanko
0,047 0,066315 3,31575
0,049 0,070122 3,50610
0,048 0,068218 3,41090
Kontrol
0,044 0,060605 3,03025
0,045 0,062509 3,12545
0,043 0,058702 2,93510
Doxo
0,077 0,123413 6,17065
0,084 0,136735 6,83675
0,079 0,127219 6,36095
EEDBB
0,037 0,047283 2,36415
0,034 0,041573 2,07865
0,033 0,039669 1,98345
EEDBB + Doxo
0,060 0,091057 4,55285
0,061 0,092961 4,64805
(19)
Lampiran 19. Contoh Perhitungan danTabel Kadar Nitrat dalam Plasma Pada kelompok blanko diperoleh absorbansi (Y) = 0,050
Persamaan regresi : Y = 0,525416X +0,012157 X = 525416 , 0 012157 , 0 Y X = 525416 , 0 012157 , 0 050 , 0
X = 0,072025
Faktor pengenceran = 50
Perhitungan kadar = X (konsentrasi) x Faktor pengenceran = 0,072025 x 50
= 3,60125
Kelompok Absorbansi (Y) Konsentrasi (X) Kadar (µ g/ml)
Blanko
0,050 0,072025 3,60125
0,051 0,073928 3,69640
0,048 0,068218 3,41090
Kontrol
0,046 0,064418 3,22090
0,045 0,062509 3,12545
0,046 0,064418 3,22090
Doxo
0,083 0,134932 6,74660
0,085 0,138639 6,93195
0,082 0,132929 6,64645
EEDBB
0,038 0,049186 2,45930
0,039 0,051089 2,55445
0,032 0,037766 1,88830
EEDBB + Doxo
0,064 0,098670 4,93350
0,065 0,100574 5,02870
(20)
Lampiran 20. Hasil Analisis ANOVA
Descriptives
Kadar Nitrat dalam Plasma
N Mean
Std.
Deviation Std. Error
95% Confidence Interval for Mean
Minimum Maximum Lower
Bound
Upper Bound
Blanko 3 3.4109167E0 .09517500 .05494931 3.1744889 3.6473445 3.31575 3.50610 Kontrol 3 3.0302667E0 .09517500 .05494931 2.7938389 3.2666945 2.93510 3.12545 Doxo 3 6.4561167E0 .34309594 .19808653 5.6038191 7.3084142 6.17065 6.83675 EEDBB 300 mg 3 2.1420833E0 .19811856 .11438380 1.6499296 2.6342371 1.98345 2.36415 EEDBB 300 mg +
Doxo 10 ml 3 4.6797500E0 .14536585 .08392701 4.3186412 5.0408588 4.55285 4.83835 Total 15 3.9438267E0 1.55945438 .40264939 3.0802296 4.8074237 1.98345 6.83675 Kadar Nitrat dalam Plasma
N Mean
Std.
Deviation Std. Error
95% Confidence Interval for Mean
Minimum Maximum Lower
Bound
Upper Bound
Blanko 3 3.5695167E0 .14537130 .08393016 3.2083943 3.9306390 3.41090 3.69640 Kontrol 3 3.1890833E0 .05510808 .03181667 3.0521873 3.3259794 3.12545 3.22090 Doxo 3 6.7750000E0 .36028932 .08363110 6.4151644 7.1348356 6.64645 6.93195 EEDBB 300 mg 3 2.3006833E0 .14485331 .20801313 1.4056751 3.1956916 1.88830 2.55445 EEDBB 300 mg +
Doxo 10 ml 3 4.9018000E0 .14536585 .08392701 4.5406912 5.2629088 4.74320 5.02870 Total 15 4.1472167E0 1.62129652 .41861696 3.2493726 5.0450608 1.88830 6.93195
ANOVA
Kadar Nitrit dalam Plasma
Sum of Squares Df Mean Square F Sig.
Between Groups 33.654 4 8.414 214.397 .000
Within Groups .392 10 .039
Total 34.047 14
Kadar Nitrat dalam Plasma
Sum of
Squares df Mean Square F Sig.
Between Groups 36.408 4 9.102 232.087 .000
Within Groups .392 10 .039
(21)
Lampiran 20. (Lanjutan)
Kadar Nitrit dalam Plasma
Perlakuan N
Subset for alpha = 0.05
1 2 3 4 5
Duncana EEDBB
300 mg 3
2.1420833 E0 Kontrol
3 3.0302667
E0 Blanko
3 3.4109167
E0 EEDBB
300 mg + Doxo 10 ml
3 4.6797500
E0
Doxo 10 ml 3 6.4561167
E0
Sig. 1.000 1.000 1.000 1.000 1.000
Kadar Nitrat dalam Plasma
Perlakuan N
Subset for alpha = 0.05
1 2 3 4 5
Duncana EEDBB
300 mg 3
2.3006833 E0 Kontrol
3 3.1890833
E0 Blanko
3 3.5695167
E0 EEDBB
300 mg + Doxo 10 ml
3 4.9018000
E0
Doxo 10 ml 3 6.7750000
(22)
Lampiran 20. (Lanjutan)
Multiple Comparisons
Kadar Nitrat dalam Plasma
(I) Perlakuan (J) Perlakuan
Mean Difference
(I-J)
Std.
Error Sig.
95% Confidence Interval Lower
Bound Upper Bound LSD Blanko Kontrol
.38065000*.1617461
3 .040 .0202572 .7410428 Doxo
-3.04520000*.1617461
3 .000 -3.4055928 -2.6848072 EEDBB 300 mg
1.26883333*.1617461
3 .000 .9084405 1.6292262 EEDBB 300 mg+
Doxo 10 ml -1.26883333
*.1617461
3 .000 -1.6292262 -.9084405 Kontrol Blanko
-.38065000*.1617461
3 .040 -.7410428 -.0202572 Doxo
-3.42585000*.1617461
3 .000 -3.7862428 -3.0654572 EEDBB 300 mg
.88818333*.1617461
3 .000 .5277905 1.2485762 EEDBB 300 mg+
Doxo 10 ml -1.64948333
*.1617461
3 .000 -2.0098762 -1.2890905 Doxo Blanko
3.04520000*.1617461
3 .000 2.6848072 3.4055928 Kontrol
3.42585000*.1617461
3 .000 3.0654572 3.7862428 EEDBB 300 mg
4.31403333*.1617461
3 .000 3.9536405 4.6744262 EEDBB 300 mg+
Doxo 10 ml 1.77636667
*.1617461
3 .000 1.4159738 2.1367595 EEDBB Blanko
-1.26883333*.1617461
3 .000 -1.6292262 -.9084405 Kontrol
-.88818333*.1617461
3 .000 -1.2485762 -.5277905 Doxo 10 ml
-4.31403333*.1617461
3 .000 -4.6744262 -3.9536405 EEDBB 300 mg+
Doxo 10 ml -2.53766667
*.1617461
3 .000 -2.8980595 -2.1772738 EEBDBB
+ Doxo
Blanko
1.26883333*.1617461
(23)
Lampiran 20. (Lanjutan)
Multiple Comparisons
Kadar Nitrat dalam Plasma
(I) Perlakuan (J) Perlakuan
Mean Difference
(I-J)
Std.
Error Sig.
95% Confidence Interval Lower
Bound Upper Bound LSD Blanko Kontrol
.38065000*.1617461
3 .040 .0202572 .7410428 Doxo
-3.04520000*.1617461
3 .000 -3.4055928 -2.6848072 EEDBB 300 mg
1.26883333*.1617461
3 .000 .9084405 1.6292262 EEDBB 300 mg+
Doxo 10 ml -1.26883333
*.1617461
3 .000 -1.6292262 -.9084405 Kontrol Blanko
-.38065000*.1617461
3 .040 -.7410428 -.0202572 Doxo
-3.42585000*.1617461
3 .000 -3.7862428 -3.0654572 EEDBB 300 mg
.88818333*.1617461
3 .000 .5277905 1.2485762 EEDBB 300 mg+
Doxo 10 ml -1.64948333
*.1617461
3 .000 -2.0098762 -1.2890905 Doxo Blanko
3.04520000*.1617461
3 .000 2.6848072 3.4055928 Kontrol
3.42585000*.1617461
3 .000 3.0654572 3.7862428 EEDBB 300 mg
4.31403333*.1617461
3 .000 3.9536405 4.6744262 EEDBB 300 mg+
Doxo 10 ml 1.77636667
*.1617461
3 .000 1.4159738 2.1367595 EEDBB Blanko
-1.26883333*.1617461
3 .000 -1.6292262 -.9084405 Kontrol
-.88818333*.1617461
3 .000 -1.2485762 -.5277905 Doxo 10 ml
-4.31403333*.1617461
3 .000 -4.6744262 -3.9536405 EEDBB 300 mg+
Doxo 10 ml -2.53766667
*.1617461
3 .000 -2.8980595 -2.1772738
(24)
Lampiran 20. (Lanjutan)
Multiple Comparisons
Kadar Nitrat dalam Plasma
(I) Perlakuan (J) Perlakuan
Mean Difference
(I-J)
Std.
Error Sig.
95% Confidence Interval Lower
Bound Upper Bound LSD Blanko Kontrol
.38043333* .1616959
4 .040 .0201523 .7407143 Doxo
-3.20548333* .1616959
4 .000 -3.5657643 -2.8452023 EEDBB 300 mg
1.26883333* .1616959
4 .000 .9085523 1.6291143 EEDBB 300 mg+
Doxo 10 ml -1.33228333
* .1616959
4 .000 -1.6925643 -.9720023 Kontrol Blanko
-.38043333* .1616959
4 .040 -.7407143 -.0201523 Doxo
-3.58591667* .1616959
4 .000 -3.9461977 -3.2256357 EEDBB 300 mg
.88840000* .1616959
4 .000 .5281190 1.2486810 EEDBB 300 mg+
Doxo 10 ml -1.71271667
* .1616959
4 .000 -2.0729977 -1.3524357 Doxo Blanko
3.20548333* .1616959
4 .000 2.8452023 3.5657643 Kontrol
3.58591667* .1616959
4 .000 3.2256357 3.9461977 EEDBB 300 mg
4.47431667* .1616959
4 .000 4.1140357 4.8345977 EEDBB 300 mg+
Doxo 10 ml 1.87320000
* .1616959
4 .000 1.5129190 2.2334810 EEDBB Blanko
-1.26883333* .1616959
4 .000 -1.6291143 -.9085523 Kontrol
-.88840000* .1616959
4 .000 -1.2486810 -.5281190 Doxo 10 ml
-4.47431667* .1616959
4 .000 -4.8345977 -4.1140357 EEDBB 300 mg+
Doxo 10 ml -2.60111667
* .1616959
4 .000 -2.9613977 -2.2408357 EEBDBB
+ Doxo
Blanko
1.33228333* .1616959
(25)
Lampiran 21. Data Orientasi Kadar Nitrit dalam Plasma
Kelompok Absorbansi Konsentrasi Kadar (µ g/ml)
Kontrol 0,047 0,066315 3,31575
0,048 0,068218 3,41090
100 mg/kgBB 0,042 0,056799 2,83995
0,041 0,054896 2,74480
200 mg/kgBB 0,040 0,052992 2,64960
0,038 0,049186 2,45930
300 mg/kgBB 0,032 0,037766 1,88830
0,034 0,041573 2,07865
400 mg/kgBB 0,028 0,030153 1,50765
0,027 0,028249 1,41245
500 mg/kgBB 0,025 0,028249 1,22215
(26)
Lampiran 22. Data Orientasi Kadar Nitrat dalam Plasma
Kelompok Absorbansi Konsentrasi Kadar (µ g/ml)
Kontrol 0,049 0,070122 3,50610
0,046 0,064418 3,22090
100 mg/kgBB 0,043 0,058702 2,93510
0,045 0,062509 3,12545
200 mg/kgBB 0,044 0,060605 3,03025
0,041 0,054896 2,74480
300 mg/kgBB 0,035 0,043476 2,17380
0,038 0,049186 2,45930
400 mg/kgBB 0,026 0,026347 1,31735
0,030 0,033959 1,69795
500 mg/kgBB 0,024 0,022540 1,12700
(1)
Lampiran 20. (Lanjutan)
Kadar Nitrit dalam Plasma
Perlakuan N
Subset for alpha = 0.05
1 2 3 4 5
Duncana EEDBB
300 mg 3
2.1420833 E0 Kontrol
3 3.0302667
E0 Blanko
3 3.4109167
E0 EEDBB
300 mg + Doxo 10 ml
3 4.6797500
E0
Doxo 10 ml 3 6.4561167
E0
Sig. 1.000 1.000 1.000 1.000 1.000
Kadar Nitrat dalam Plasma
Perlakuan N
Subset for alpha = 0.05
1 2 3 4 5
Duncana EEDBB
300 mg 3
2.3006833 E0 Kontrol
3 3.1890833
E0 Blanko
3 3.5695167
E0 EEDBB
300 mg + Doxo 10 ml
3 4.9018000
E0
Doxo 10 ml 3 6.7750000
E0
Sig. 1.000 1.000 1.000 1.000 1.000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
(2)
71
Lampiran 20. (Lanjutan)
Multiple Comparisons
Kadar Nitrat dalam Plasma
(I) Perlakuan (J) Perlakuan
Mean Difference
(I-J)
Std.
Error Sig.
95% Confidence Interval Lower
Bound Upper Bound LSD Blanko Kontrol
.38065000*.1617461
3 .040 .0202572 .7410428 Doxo
-3.04520000*.1617461
3 .000 -3.4055928 -2.6848072 EEDBB 300 mg
1.26883333*.1617461
3 .000 .9084405 1.6292262 EEDBB 300 mg+
Doxo 10 ml -1.26883333
*.1617461
3 .000 -1.6292262 -.9084405 Kontrol Blanko
-.38065000*.1617461
3 .040 -.7410428 -.0202572 Doxo
-3.42585000*.1617461
3 .000 -3.7862428 -3.0654572 EEDBB 300 mg
.88818333*.1617461
3 .000 .5277905 1.2485762 EEDBB 300 mg+
Doxo 10 ml -1.64948333
*.1617461
3 .000 -2.0098762 -1.2890905 Doxo Blanko
3.04520000*.1617461
3 .000 2.6848072 3.4055928 Kontrol
3.42585000*.1617461
3 .000 3.0654572 3.7862428 EEDBB 300 mg
4.31403333*.1617461
3 .000 3.9536405 4.6744262 EEDBB 300 mg+
Doxo 10 ml 1.77636667
*.1617461
3 .000 1.4159738 2.1367595 EEDBB Blanko
-1.26883333*.1617461
3 .000 -1.6292262 -.9084405 Kontrol
-.88818333*.1617461
3 .000 -1.2485762 -.5277905 Doxo 10 ml
-4.31403333*.1617461
3 .000 -4.6744262 -3.9536405 EEDBB 300 mg+
Doxo 10 ml -2.53766667
*.1617461
3 .000 -2.8980595 -2.1772738 EEBDBB
+ Doxo
Blanko
1.26883333*.1617461
3 .000 .9084405 1.6292262 Kontrol
1.64948333*.1617461
3 .000 1.2890905 2.0098762 Doxo 10 ml
-1.77636667*.1617461
3 .000 -2.1367595 -1.4159738 EEDBB 300 mg
2.53766667*.1617461
3 .000 2.1772738 2.8980595
(3)
Lampiran 20. (Lanjutan)
Multiple Comparisons
Kadar Nitrat dalam Plasma
(I) Perlakuan (J) Perlakuan
Mean Difference
(I-J)
Std.
Error Sig.
95% Confidence Interval Lower
Bound Upper Bound LSD Blanko Kontrol
.38065000*.1617461
3 .040 .0202572 .7410428 Doxo
-3.04520000*.1617461
3 .000 -3.4055928 -2.6848072 EEDBB 300 mg
1.26883333*.1617461
3 .000 .9084405 1.6292262 EEDBB 300 mg+
Doxo 10 ml -1.26883333
*.1617461
3 .000 -1.6292262 -.9084405 Kontrol Blanko
-.38065000*.1617461
3 .040 -.7410428 -.0202572 Doxo
-3.42585000*.1617461
3 .000 -3.7862428 -3.0654572 EEDBB 300 mg
.88818333*.1617461
3 .000 .5277905 1.2485762 EEDBB 300 mg+
Doxo 10 ml -1.64948333
*.1617461
3 .000 -2.0098762 -1.2890905 Doxo Blanko
3.04520000*.1617461
3 .000 2.6848072 3.4055928 Kontrol
3.42585000*.1617461
3 .000 3.0654572 3.7862428 EEDBB 300 mg
4.31403333*.1617461
3 .000 3.9536405 4.6744262 EEDBB 300 mg+
Doxo 10 ml 1.77636667
*.1617461
3 .000 1.4159738 2.1367595 EEDBB Blanko
-1.26883333*.1617461
3 .000 -1.6292262 -.9084405 Kontrol
-.88818333*.1617461
3 .000 -1.2485762 -.5277905 Doxo 10 ml
-4.31403333*.1617461
3 .000 -4.6744262 -3.9536405 EEDBB 300 mg+
Doxo 10 ml -2.53766667
*.1617461
3 .000 -2.8980595 -2.1772738 EEBDBB
+ Doxo
Blanko
1.26883333*.1617461
3 .000 .9084405 1.6292262 Kontrol
1.64948333*.1617461
3 .000 1.2890905 2.0098762 Doxo 10 ml
-1.77636667*.1617461
3 .000 -2.1367595 -1.4159738 EEDBB 300 mg
2.53766667*.1617461
3 .000 2.1772738 2.8980595
*. The mean difference is significant at the 0.05 level.
(4)
71
Lampiran 20. (Lanjutan)
Multiple Comparisons
Kadar Nitrat dalam Plasma
(I) Perlakuan (J) Perlakuan
Mean Difference
(I-J)
Std.
Error Sig.
95% Confidence Interval Lower
Bound Upper Bound LSD Blanko Kontrol
.38043333* .1616959
4 .040 .0201523 .7407143 Doxo
-3.20548333* .1616959
4 .000 -3.5657643 -2.8452023 EEDBB 300 mg
1.26883333* .1616959
4 .000 .9085523 1.6291143 EEDBB 300 mg+
Doxo 10 ml -1.33228333
* .1616959
4 .000 -1.6925643 -.9720023 Kontrol Blanko
-.38043333* .1616959
4 .040 -.7407143 -.0201523 Doxo
-3.58591667* .1616959
4 .000 -3.9461977 -3.2256357 EEDBB 300 mg
.88840000* .1616959
4 .000 .5281190 1.2486810 EEDBB 300 mg+
Doxo 10 ml -1.71271667
* .1616959
4 .000 -2.0729977 -1.3524357 Doxo Blanko
3.20548333* .1616959
4 .000 2.8452023 3.5657643 Kontrol
3.58591667* .1616959
4 .000 3.2256357 3.9461977 EEDBB 300 mg
4.47431667* .1616959
4 .000 4.1140357 4.8345977 EEDBB 300 mg+
Doxo 10 ml 1.87320000
* .1616959
4 .000 1.5129190 2.2334810 EEDBB Blanko
-1.26883333* .1616959
4 .000 -1.6291143 -.9085523 Kontrol
-.88840000* .1616959
4 .000 -1.2486810 -.5281190 Doxo 10 ml
-4.47431667* .1616959
4 .000 -4.8345977 -4.1140357 EEDBB 300 mg+
Doxo 10 ml -2.60111667
* .1616959
4 .000 -2.9613977 -2.2408357 EEBDBB
+ Doxo
Blanko
1.33228333* .1616959
4 .000 .9720023 1.6925643 Kontrol
1.71271667* .1616959
4 .000 1.3524357 2.0729977 Doxo 10 ml
-1.87320000* .1616959
4 .000 -2.2334810 -1.5129190 EEDBB 300 mg
2.60111667* .1616959
4 .000 2.2408357 2.9613977
*. The mean difference is significant at the 0.05 level.
(5)
Lampiran 21. Data Orientasi Kadar Nitrit dalam Plasma
Kelompok
Absorbansi
Konsentrasi
Kadar (µ g/ml)
Kontrol
0,047
0,066315
3,31575
0,048
0,068218
3,41090
100 mg/kgBB
0,042
0,056799
2,83995
0,041
0,054896
2,74480
200 mg/kgBB
0,040
0,052992
2,64960
0,038
0,049186
2,45930
300 mg/kgBB
0,032
0,037766
1,88830
0,034
0,041573
2,07865
400 mg/kgBB
0,028
0,030153
1,50765
0,027
0,028249
1,41245
500 mg/kgBB
0,025
0,028249
1,22215
(6)