Uji Toksisitas Akut Oral Ekstrak Etanol Daun Srikaya (Annona squamosa L.) Terhadap Mencit (Mus musculus)
Lampiran 1. Surat hasil identifikasi sampel
54
Universitas Sumatera Utara
Lampiran 2. Gambar tumbuhan dan daun segar srikaya (Annona squamosa L.)
Tumbuhan srikaya
Daun segar tumbuhan srikaya
55
Universitas Sumatera Utara
Lampiran 3. Gambar ekstrak etanol daun srikaya (EEDS)
EEDS
Suspensi EEDS
56
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Lampiran 4. Rekomendasi persetujuan etik penelitian kesehatan
57
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Lampiran 5. Bagan penelitian uji toksisitas ekstrak etanol daun srikaya metode fixed dose
Uji Pendahuluan
Dosis awal: 5 mg/kg bb
mulai
1 hewan
300 mg/kg bb
1 hewan
50 mg/kg bb
1 hewan
5 mg/kg bb
C
C
C
:
C
2000
58
Uji utama dengan dosis awal (mg/kg)
1 hewan
2000 mg/kg bb
Keterangan:
Dosis awal: 2000 mg/kg bb
A
≥ 2 mati
B
≥ 1 menunjukkan gejala toksisitas dan tidak
ada kematian
C
= tidak ada gejala toksisitas
Klasifikasi kategori GHS
Uji Utama
mulai
:
5 hewan
2000 mg/kg bb
5 hewan
5000 mg/kg bb
C
C
5/tidak terklasifikasi
Universitas Sumatera Utara
Lampiran 6. Hewan uji
Uji pendahuluan
Uji utama
59
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Lampiran 7. Contoh perhitungan dosis
Contoh perhitungan EEDS yang akan diberikan kepada mencit
-
Dosis suspensi EEDS yang akan dibuat adalah 5, 50, 300, 2000, 5000
mg/kg bb.
-
Cara pembuatan suspensi EEDS:
Timbang 5, 50, 300, 2000, 5000 mg EEDS, masing-masing disuspensikan
dalam 10 ml suspensi Na-CMC 0,5%.
-
Volume suspensi EEDS yang diberikan:
Contoh bb mencit = 25 mg
Jumlah EEDS dosis 5 mg/kg bb =
Volume larutan yang diberikan =
0,125
5
Jumlah EEDS dosis 50 mg/kg bb =
Volume larutan yang diberikan =
50
�
× 25 � = 1,25
�
× 10
�
�
50
300
7,5
× 10
�
1000 �
300
50
2000
Jumlah EEDS dosis 5000 mg/kg bb =
Volume larutan yang diberikan =
�
�
1,25
Jumlah EEDS dosis 2000 mg/kg bb =
Volume larutan yang diberikan =
�
× 25 � = 0,125
1000 �
Jumlah EEDS dosis 300 mg/kg bb =
Volume larutan yang diberikan =
�
5
1000 �
125
5000
�
�
× 25 � = 7,5
�
1000 �
�
�
5000
�
= 0,25
�
�
× 25 � = 50
× 10
1000 �
�
= 0,25
× 10
2000
= 0,25
= 0,25
× 25 � = 125
× 10
= 0,25
�
�
60
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Lampiran 8. Hasil analisis data SPSS berat badan mencit
a.
Uji pendahuluan
Tests of Normality
Kolmogorov-Smirnova
Shapiro-Wilk
Kelompok
Statistic df Sig. Statistic df Sig.
BeratBadan CMC Na 0,5%
.180 7
.200*
.977 7 .942
*
EEDS 50 mg/kg bb
.200 7
.200
.960 7 .822
*
EEDS 100 mg/kg bb
.209 7
.200
.865 7 .168
EEDS 300 mg/kg bb
.279 7
.105
.812 7 .054
EEDS 2000 mg/kg bb
.273 7
.125
.843 7 .106
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
BeratBadan
CMC Na
0,5%
EEDS 50
mg/kg bb
EEDS 100
mg/kg bb
EEDS 300
mg/kg bb
EEDS 2000
mg/kg bb
Total
95% Confidence
Interval for Mean
Std.
Std. Lower Upper
N Mean Deviation Error Bound Bound Minimum Maximum
7 30.014
.9008 .3405 29.181 30.847
28.5
31.4
7 29.600
.7416 .2803 28.914 30.286
28.3
30.7
7 29.971
1.0547 .3986 28.996 30.947
29.0
31.8
7 30.114
.9477 .3582 29.238 30.991
29.2
32.1
7 30.600
1.4059 .5314 29.300 31.900
29.1
33.5
35 30.060
1.0248 .1732 29.708 30.412
28.3
33.5
61
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Lampiran 8. (lanjutan)
ANOVA
BeratBadan
Between Groups
Within Groups
Total
Sum of
Squares
3.613
32.091
35.704
Df
Mean Square
4
.903
30
1.070
34
F
.844
Sig.
.508
Multiple Comparisons
BeratBadan
Tukey HSD
(I) Kelompok (J) Kelompok
CMC Na 0,5% EEDS 50
mg/kg bb
EEDS 100
mg/kg bb
EEDS 300
mg/kg bb
EEDS 2000
mg/kg bb
EEDS 50
CMC Na 0,5%
mg/kg bb
EEDS 100
mg/kg bb
EEDS 300
mg/kg bb
EEDS 2000
mg/kg bb
Mean
Difference Std.
(I-J)
Error Sig.
.4143 .5528
.943
95% Confidence
Interval
Lower
Upper
Bound
Bound
-1.189
2.018
.0429
.5528
1.000
-1.561
1.646
-.1000
.5528
1.000
-1.704
1.504
-.5857
.5528
.825
-2.189
1.018
-.4143
-.3714
.5528
.5528
.943
.961
-2.018
-1.975
1.189
1.232
-.5143
.5528
.883
-2.118
1.089
-1.0000
.5528
.387
-2.604
.604
62
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Lampiran 8. (lanjutan)
(I) Kelompok
EEDS 100
mg/kg bb
EEDS 300
mg/kg bb
EEDS 2000
mg/kg bb
Mean
Difference Std.
(J) Kelompok
(I-J)
Error Sig.
CMC Na 0,5%
-.0429 .5528 1.000
EEDS 50
.3714 .5528
.961
mg/kg bb
EEDS 300
-.1429 .5528
.999
mg/kg bb
EEDS 2000
-.6286 .5528
.786
mg/kg bb
CMC Na 0,5%
.1000 .5528 1.000
EEDS 50
.5143 .5528
.883
mg/kg bb
EEDS 100
.1429 .5528
.999
mg/kg bb
EEDS 2000
-.4857 .5528
.903
mg/kg bb
CMC Na 0,5%
.5857 .5528
.825
EEDS 50
1.0000 .5528
.387
mg/kg bb
EEDS 100
.6286 .5528
.786
mg/kg bb
EEDS 300
.4857 .5528
.903
mg/kg bb
95% Confidence
Interval
Lower
Upper
Bound
Bound
-1.646
1.561
-1.232
1.975
-1.746
1.461
-2.232
.975
-1.504
-1.089
1.704
2.118
-1.461
1.746
-2.089
1.118
-1.018
-.604
2.189
2.604
-.975
2.232
-1.118
2.089
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Lampiran 8. (lanjutan)
BeratBadan
Tukey HSDa
Kelompok
N
EEDS 50 mg/kg bb
7
EEDS 100 mg/kg bb
7
CMC Na 0,5%
7
EEDS 300 mg/kg bb
7
EEDS 2000 mg/kg bb
7
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 7.000.
b.
Subset for alpha = 0.05
1
29.600
29.971
30.014
30.114
30.600
.387
Uji utama
Tests of Normality
Kolmogorov-Smirnova
Shapiro-Wilk
Statistic
df
Sig. Statistic
df
Sig.
.137
35
.094
.949
35
.105
*
.114
35
.200
.956
35
.169
Kelompok
BeratBadan CMC Na 0,5%
EEDS 2000
mg/kg bb
EEDS 5000
.123
35
mg/kg bb
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
.198
.961
35
.241
64
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Lampiran 8. (lanjutan)
Descriptives
BeratBadan
CMC Na
0,5%
EEDS 2000
mg/kg bb
EEDS 5000
mg/kg bb
Total
95% Confidence
Interval for Mean
Std.
Std. Lower Upper
N Mean Deviation Error Bound Bound Minimum Maximum
35 29.011 1.4834 .2507 28.502 29.521
26.5
32.3
35 27.831
2.5537 .4317 26.954 28.709
23.8
33.5
35 28.486
2.2488 .3801 27.713 29.258
24.7
34.4
105 28.443
2.1771 .2125 28.022 28.864
23.8
34.4
F
2.663
Sig.
.075
ANOVA
BeratBadan
Between Groups
Within Groups
Total
Sum of
Squares
24.463
468.494
492.957
df
Mean Square
2
12.232
102
4.593
104
Multiple Comparisons
BeratBadan
Tukey HSD
(I) Kelompok (J) Kelompok
CMC Na 0,5% EEDS 2000
mg/kg bb
EEDS 5000
mg/kg bb
EEDS 2000
CMC Na 0,5%
mg/kg bb
EEDS 5000
mg/kg bb
Mean
Difference Std.
(I-J)
Error Sig.
1.1800 .5123
.060
95% Confidence
Interval
Lower
Upper
Bound
Bound
-.038
2.398
.5257
.5123
.562
-.693
1.744
-1.1800
-.6543
.5123
.5123
.060
.411
-2.398
-1.873
.038
.564
65
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Lampiran 8. (lanjutan)
(I) Kelompok
EEDS 5000
mg/kg bb
Mean
Difference Std.
(J) Kelompok
(I-J)
Error Sig.
CMC Na 0,5%
-.5257 .5123
.562
EEDS 2000
.6543 .5123
.411
mg/kg bb
95% Confidence
Interval
Lower
Upper
Bound
Bound
-1.744
.693
-.564
1.873
BeratBadan
Tukey HSD
a
Subset for alpha = 0.05
Kelompok
N
1
EEDS 2000 mg/kg bb
35
27.831
EEDS 5000 mg/kg bb
35
28.486
CMC Na 0,5%
35
29.011
Sig.
.060
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 35.000.
66
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Lampiran 9. Hasil analisis data SPSS berat organ relatif
a.
Berat organ relatif hati
Tests of Normality
Kolmogorov-Smirnova
Kelompok
Statistic
CMC Na 0,5%
.230
Berat organ
relatif hati
Df
EEDS 2000
.253
mg/kg bb
EEDS 5000
.279
mg/kg bb
a. Lilliefors Significance Correction
Shapiro-Wilk
Sig. Statistic
3
.
.981
df
Sig.
3
.736
3
.
.964
3
.637
3
.
.939
3
.523
Descriptives
Berat organ relatif hati
CMC Na
0,5%
EEDS 2000
mg/kg bb
95% Confidence
Interval for
Mean
Std.
Std. Lower Upper
N Mean Deviation Error Bound Bound Minimum Maximum
3 5.1900 .94398 .54501 2.8450 7.5350
4.18
6.05
3 5.7633
.06110 .03528 5.6116 5.9151
5.71
5.83
EEDS 5000
mg/kg bb
3 5.0033
.75857 .43796 3.1189 6.8877
4.16
5.63
Total
9 5.3189
.69657 .23219 4.7835 5.8543
4.16
6.05
ANOVA
Berat organ relatif hati
Between Groups
Sum of
Squares
.941
2
Mean
Square
.471
.490
Df
Within Groups
2.941
6
Total
3.882
8
F
Sig.
.960
.435
67
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Lampiran 9. (lanjutan)
Multiple Comparisons
Berat organ relatif hati
Tukey HSD
(I) Kelompok (J) Kelompok
CMC Na 0,5% EEDS 2000
mg/kg bb
EEDS 2000
mg/kg bb
EEDS 5000
mg/kg bb
Mean
Difference Std.
(I-J)
Error Sig.
-.57333 .57160
.602
95% Confidence
Interval
Lower
Upper
Bound
Bound
-2.3272
1.1805
EEDS 5000
mg/kg bb
CMC Na 0,5%
.18667 .57160
.943
-1.5672
1.9405
.57333 .57160
.602
-1.1805
2.3272
EEDS 5000
mg/kg bb
CMC Na 0,5%
EEDS 2000
mg/kg bb
.76000 .57160
.431
-.9938
2.5138
-.18667 .57160
-.76000 .57160
.943
.431
-1.9405
-2.5138
1.5672
.9938
Berat organ relatif hati
Tukey HSDa
Kelompok
EEDS 5000 mg/kg bb
N
Subset for alpha = 0.05
1
3
5.0033
CMC Na 0,5%
3
5.1900
EEDS 2000 mg/kg bb
3
5.7633
Sig.
.431
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
68
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Lampiran 9. (lanjutan)
b.
Berat organ retatif ginjal kanan
Tests of Normality
Kolmogorov-Smirnova
Kelompok
Statistic
Berat organ relatif CMC Na 0,5%
.333
ginjal kanan
EEDS 2000
.314
mg/kg bb
EEDS 5000
.292
mg/kg bb
a. Lilliefors Significance Correction
df
Shapiro-Wilk
Sig. Statistic
3
.
.862
df
Sig.
3 .274
3
.
.893
3
.363
3
.
.923
3
.463
Descriptives
Berat organ relatif ginjal kanan
CMC Na
0,5%
EEDS 2000
mg/kg bb
95% Confidence
Interval for
Mean
Std.
Std. Lower Upper
N Mean Deviation Error Bound Bound Minimum Maximum
3 .6000 .07000 .04041 .4261
.7739
.52
.65
3 .5700
.02646 .01528
.5043
.6357
.54
.59
EEDS 5000
mg/kg bb
3 .5633
.02082 .01202
.5116
.6150
.54
.58
Total
9 .5778
.04236 .01412
.5452
.6103
.52
.65
.569
Sig.
.594
ANOVA
Berat organ relatif ginjal kanan
Between Groups
Sum of
Squares
.002
df
Mean Square
2
.001
Within Groups
.012
6
Total
.014
8
F
.002
69
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Lampiran 9. (lanjutan)
Multiple Comparisons
Berat organ relatif ginjal kanan
Tukey HSD
(I) Kelompok (J) Kelompok
CMC Na 0,5% EEDS 2000
mg/kg bb
EEDS 5000
mg/kg bb
CMC Na 0,5%
EEDS 2000
mg/kg bb
EEDS 5000
mg/kg bb
CMC Na 0,5%
EEDS 5000
mg/kg bb
EEDS 2000
mg/kg bb
Mean
Difference Std.
(I-J)
Error Sig.
.03000 .03662
.706
95% Confidence
Interval
Lower
Upper
Bound
Bound
-.0823
.1423
.03667 .03662
.603
-.0757
.1490
-.03000 .03662
.706
-.1423
.0823
.00667 .03662
.982
-.1057
.1190
-.03667 .03662
.603
-.1490
.0757
-.00667 .03662
.982
-.1190
.1057
Berat organ relatif ginjal kanan
Tukey HSD
a
Subset for alpha = 0.05
Kelompok
N
1
EEDS 5000 mg/kg bb
3
.5633
EEDS 2000 mg/kg bb
3
.5700
CMC Na 0,5%
3
.6000
Sig.
.603
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
70
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Lampiran 9. (lanjutan)
c.
Berat organ relatif ginjal kiri
Tests of Normality
Kolmogorov-Smirnova
Kelompok
Statistic
Berat organ relatif CMC Na 0,5%
.216
ginjal kiri
EEDS 2000
.304
mg/kg bb
EEDS 5000
.292
mg/kg bb
a. Lilliefors Significance Correction
df
Shapiro-Wilk
Sig. Statistic
3
.
.989
df
Sig.
3 .797
3
.
.907
3
.407
3
.
.923
3
.463
Descriptives
Berat organ relatif ginjal kiri
CMC Na
0,5%
EEDS 2000
mg/kg bb
95% Confidence
Interval for
Mean
Std.
Std. Lower Upper
N Mean Deviation Error Bound Bound Minimum Maximum
3 .5767 .13577 .07839 .2394
.9139
.45
.72
3 .5933
.04726 .02728
.4759
.7107
.54
.63
EEDS 5000
mg/kg bb
3 .5567
.02082 .01202
.5050
.6084
.54
.58
Total
9 .5756
.07435 .02478
.5184
.6327
.45
.72
.144
Sig.
.869
ANOVA
Berat organ relatif ginjal kiri
Between Groups
Sum of
Squares
.002
df
Mean Square
2
.001
Within Groups
.042
6
Total
.044
8
F
.007
71
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Lampiran 9. (lanjutan)
Multiple Comparisons
Berat organ relatif ginjal kiri
Tukey HSD
(I) Kelompok (J) Kelompok
CMC Na 0,5% EEDS 2000
mg/kg bb
EEDS 2000
mg/kg bb
EEDS 5000
mg/kg bb
Mean
Difference Std.
(I-J)
Error Sig.
-.01667 .06848
.968
95% Confidence
Interval
Lower
Upper
Bound
Bound
-.2268
.1934
EEDS 5000
mg/kg bb
CMC Na 0,5%
.02000 .06848
.954
-.1901
.2301
.01667 .06848
.968
-.1934
.2268
EEDS 5000
mg/kg bb
CMC Na 0,5%
.03667 .06848
.857
-.1734
.2468
-.02000 .06848
.954
-.2301
.1901
-.03667 .06848
.857
-.2468
.1734
EEDS 2000
mg/kg bb
Berat organ relatif ginjal kiri
Tukey HSD
a
Subset for alpha = 0.05
Kelompok
N
1
EEDS 5000 mg/kg bb
3
.5567
CMC Na 0,5%
3
.5767
EEDS 2000 mg/kg bb
3
.5933
Sig.
.857
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
72
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54
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Lampiran 2. Gambar tumbuhan dan daun segar srikaya (Annona squamosa L.)
Tumbuhan srikaya
Daun segar tumbuhan srikaya
55
Universitas Sumatera Utara
Lampiran 3. Gambar ekstrak etanol daun srikaya (EEDS)
EEDS
Suspensi EEDS
56
Universitas Sumatera Utara
Lampiran 4. Rekomendasi persetujuan etik penelitian kesehatan
57
Universitas Sumatera Utara
Lampiran 5. Bagan penelitian uji toksisitas ekstrak etanol daun srikaya metode fixed dose
Uji Pendahuluan
Dosis awal: 5 mg/kg bb
mulai
1 hewan
300 mg/kg bb
1 hewan
50 mg/kg bb
1 hewan
5 mg/kg bb
C
C
C
:
C
2000
58
Uji utama dengan dosis awal (mg/kg)
1 hewan
2000 mg/kg bb
Keterangan:
Dosis awal: 2000 mg/kg bb
A
≥ 2 mati
B
≥ 1 menunjukkan gejala toksisitas dan tidak
ada kematian
C
= tidak ada gejala toksisitas
Klasifikasi kategori GHS
Uji Utama
mulai
:
5 hewan
2000 mg/kg bb
5 hewan
5000 mg/kg bb
C
C
5/tidak terklasifikasi
Universitas Sumatera Utara
Lampiran 6. Hewan uji
Uji pendahuluan
Uji utama
59
Universitas Sumatera Utara
Lampiran 7. Contoh perhitungan dosis
Contoh perhitungan EEDS yang akan diberikan kepada mencit
-
Dosis suspensi EEDS yang akan dibuat adalah 5, 50, 300, 2000, 5000
mg/kg bb.
-
Cara pembuatan suspensi EEDS:
Timbang 5, 50, 300, 2000, 5000 mg EEDS, masing-masing disuspensikan
dalam 10 ml suspensi Na-CMC 0,5%.
-
Volume suspensi EEDS yang diberikan:
Contoh bb mencit = 25 mg
Jumlah EEDS dosis 5 mg/kg bb =
Volume larutan yang diberikan =
0,125
5
Jumlah EEDS dosis 50 mg/kg bb =
Volume larutan yang diberikan =
50
�
× 25 � = 1,25
�
× 10
�
�
50
300
7,5
× 10
�
1000 �
300
50
2000
Jumlah EEDS dosis 5000 mg/kg bb =
Volume larutan yang diberikan =
�
�
1,25
Jumlah EEDS dosis 2000 mg/kg bb =
Volume larutan yang diberikan =
�
× 25 � = 0,125
1000 �
Jumlah EEDS dosis 300 mg/kg bb =
Volume larutan yang diberikan =
�
5
1000 �
125
5000
�
�
× 25 � = 7,5
�
1000 �
�
�
5000
�
= 0,25
�
�
× 25 � = 50
× 10
1000 �
�
= 0,25
× 10
2000
= 0,25
= 0,25
× 25 � = 125
× 10
= 0,25
�
�
60
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Lampiran 8. Hasil analisis data SPSS berat badan mencit
a.
Uji pendahuluan
Tests of Normality
Kolmogorov-Smirnova
Shapiro-Wilk
Kelompok
Statistic df Sig. Statistic df Sig.
BeratBadan CMC Na 0,5%
.180 7
.200*
.977 7 .942
*
EEDS 50 mg/kg bb
.200 7
.200
.960 7 .822
*
EEDS 100 mg/kg bb
.209 7
.200
.865 7 .168
EEDS 300 mg/kg bb
.279 7
.105
.812 7 .054
EEDS 2000 mg/kg bb
.273 7
.125
.843 7 .106
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Descriptives
BeratBadan
CMC Na
0,5%
EEDS 50
mg/kg bb
EEDS 100
mg/kg bb
EEDS 300
mg/kg bb
EEDS 2000
mg/kg bb
Total
95% Confidence
Interval for Mean
Std.
Std. Lower Upper
N Mean Deviation Error Bound Bound Minimum Maximum
7 30.014
.9008 .3405 29.181 30.847
28.5
31.4
7 29.600
.7416 .2803 28.914 30.286
28.3
30.7
7 29.971
1.0547 .3986 28.996 30.947
29.0
31.8
7 30.114
.9477 .3582 29.238 30.991
29.2
32.1
7 30.600
1.4059 .5314 29.300 31.900
29.1
33.5
35 30.060
1.0248 .1732 29.708 30.412
28.3
33.5
61
Universitas Sumatera Utara
Lampiran 8. (lanjutan)
ANOVA
BeratBadan
Between Groups
Within Groups
Total
Sum of
Squares
3.613
32.091
35.704
Df
Mean Square
4
.903
30
1.070
34
F
.844
Sig.
.508
Multiple Comparisons
BeratBadan
Tukey HSD
(I) Kelompok (J) Kelompok
CMC Na 0,5% EEDS 50
mg/kg bb
EEDS 100
mg/kg bb
EEDS 300
mg/kg bb
EEDS 2000
mg/kg bb
EEDS 50
CMC Na 0,5%
mg/kg bb
EEDS 100
mg/kg bb
EEDS 300
mg/kg bb
EEDS 2000
mg/kg bb
Mean
Difference Std.
(I-J)
Error Sig.
.4143 .5528
.943
95% Confidence
Interval
Lower
Upper
Bound
Bound
-1.189
2.018
.0429
.5528
1.000
-1.561
1.646
-.1000
.5528
1.000
-1.704
1.504
-.5857
.5528
.825
-2.189
1.018
-.4143
-.3714
.5528
.5528
.943
.961
-2.018
-1.975
1.189
1.232
-.5143
.5528
.883
-2.118
1.089
-1.0000
.5528
.387
-2.604
.604
62
Universitas Sumatera Utara
Lampiran 8. (lanjutan)
(I) Kelompok
EEDS 100
mg/kg bb
EEDS 300
mg/kg bb
EEDS 2000
mg/kg bb
Mean
Difference Std.
(J) Kelompok
(I-J)
Error Sig.
CMC Na 0,5%
-.0429 .5528 1.000
EEDS 50
.3714 .5528
.961
mg/kg bb
EEDS 300
-.1429 .5528
.999
mg/kg bb
EEDS 2000
-.6286 .5528
.786
mg/kg bb
CMC Na 0,5%
.1000 .5528 1.000
EEDS 50
.5143 .5528
.883
mg/kg bb
EEDS 100
.1429 .5528
.999
mg/kg bb
EEDS 2000
-.4857 .5528
.903
mg/kg bb
CMC Na 0,5%
.5857 .5528
.825
EEDS 50
1.0000 .5528
.387
mg/kg bb
EEDS 100
.6286 .5528
.786
mg/kg bb
EEDS 300
.4857 .5528
.903
mg/kg bb
95% Confidence
Interval
Lower
Upper
Bound
Bound
-1.646
1.561
-1.232
1.975
-1.746
1.461
-2.232
.975
-1.504
-1.089
1.704
2.118
-1.461
1.746
-2.089
1.118
-1.018
-.604
2.189
2.604
-.975
2.232
-1.118
2.089
63
Universitas Sumatera Utara
Lampiran 8. (lanjutan)
BeratBadan
Tukey HSDa
Kelompok
N
EEDS 50 mg/kg bb
7
EEDS 100 mg/kg bb
7
CMC Na 0,5%
7
EEDS 300 mg/kg bb
7
EEDS 2000 mg/kg bb
7
Sig.
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 7.000.
b.
Subset for alpha = 0.05
1
29.600
29.971
30.014
30.114
30.600
.387
Uji utama
Tests of Normality
Kolmogorov-Smirnova
Shapiro-Wilk
Statistic
df
Sig. Statistic
df
Sig.
.137
35
.094
.949
35
.105
*
.114
35
.200
.956
35
.169
Kelompok
BeratBadan CMC Na 0,5%
EEDS 2000
mg/kg bb
EEDS 5000
.123
35
mg/kg bb
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
.198
.961
35
.241
64
Universitas Sumatera Utara
Lampiran 8. (lanjutan)
Descriptives
BeratBadan
CMC Na
0,5%
EEDS 2000
mg/kg bb
EEDS 5000
mg/kg bb
Total
95% Confidence
Interval for Mean
Std.
Std. Lower Upper
N Mean Deviation Error Bound Bound Minimum Maximum
35 29.011 1.4834 .2507 28.502 29.521
26.5
32.3
35 27.831
2.5537 .4317 26.954 28.709
23.8
33.5
35 28.486
2.2488 .3801 27.713 29.258
24.7
34.4
105 28.443
2.1771 .2125 28.022 28.864
23.8
34.4
F
2.663
Sig.
.075
ANOVA
BeratBadan
Between Groups
Within Groups
Total
Sum of
Squares
24.463
468.494
492.957
df
Mean Square
2
12.232
102
4.593
104
Multiple Comparisons
BeratBadan
Tukey HSD
(I) Kelompok (J) Kelompok
CMC Na 0,5% EEDS 2000
mg/kg bb
EEDS 5000
mg/kg bb
EEDS 2000
CMC Na 0,5%
mg/kg bb
EEDS 5000
mg/kg bb
Mean
Difference Std.
(I-J)
Error Sig.
1.1800 .5123
.060
95% Confidence
Interval
Lower
Upper
Bound
Bound
-.038
2.398
.5257
.5123
.562
-.693
1.744
-1.1800
-.6543
.5123
.5123
.060
.411
-2.398
-1.873
.038
.564
65
Universitas Sumatera Utara
Lampiran 8. (lanjutan)
(I) Kelompok
EEDS 5000
mg/kg bb
Mean
Difference Std.
(J) Kelompok
(I-J)
Error Sig.
CMC Na 0,5%
-.5257 .5123
.562
EEDS 2000
.6543 .5123
.411
mg/kg bb
95% Confidence
Interval
Lower
Upper
Bound
Bound
-1.744
.693
-.564
1.873
BeratBadan
Tukey HSD
a
Subset for alpha = 0.05
Kelompok
N
1
EEDS 2000 mg/kg bb
35
27.831
EEDS 5000 mg/kg bb
35
28.486
CMC Na 0,5%
35
29.011
Sig.
.060
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 35.000.
66
Universitas Sumatera Utara
Lampiran 9. Hasil analisis data SPSS berat organ relatif
a.
Berat organ relatif hati
Tests of Normality
Kolmogorov-Smirnova
Kelompok
Statistic
CMC Na 0,5%
.230
Berat organ
relatif hati
Df
EEDS 2000
.253
mg/kg bb
EEDS 5000
.279
mg/kg bb
a. Lilliefors Significance Correction
Shapiro-Wilk
Sig. Statistic
3
.
.981
df
Sig.
3
.736
3
.
.964
3
.637
3
.
.939
3
.523
Descriptives
Berat organ relatif hati
CMC Na
0,5%
EEDS 2000
mg/kg bb
95% Confidence
Interval for
Mean
Std.
Std. Lower Upper
N Mean Deviation Error Bound Bound Minimum Maximum
3 5.1900 .94398 .54501 2.8450 7.5350
4.18
6.05
3 5.7633
.06110 .03528 5.6116 5.9151
5.71
5.83
EEDS 5000
mg/kg bb
3 5.0033
.75857 .43796 3.1189 6.8877
4.16
5.63
Total
9 5.3189
.69657 .23219 4.7835 5.8543
4.16
6.05
ANOVA
Berat organ relatif hati
Between Groups
Sum of
Squares
.941
2
Mean
Square
.471
.490
Df
Within Groups
2.941
6
Total
3.882
8
F
Sig.
.960
.435
67
Universitas Sumatera Utara
Lampiran 9. (lanjutan)
Multiple Comparisons
Berat organ relatif hati
Tukey HSD
(I) Kelompok (J) Kelompok
CMC Na 0,5% EEDS 2000
mg/kg bb
EEDS 2000
mg/kg bb
EEDS 5000
mg/kg bb
Mean
Difference Std.
(I-J)
Error Sig.
-.57333 .57160
.602
95% Confidence
Interval
Lower
Upper
Bound
Bound
-2.3272
1.1805
EEDS 5000
mg/kg bb
CMC Na 0,5%
.18667 .57160
.943
-1.5672
1.9405
.57333 .57160
.602
-1.1805
2.3272
EEDS 5000
mg/kg bb
CMC Na 0,5%
EEDS 2000
mg/kg bb
.76000 .57160
.431
-.9938
2.5138
-.18667 .57160
-.76000 .57160
.943
.431
-1.9405
-2.5138
1.5672
.9938
Berat organ relatif hati
Tukey HSDa
Kelompok
EEDS 5000 mg/kg bb
N
Subset for alpha = 0.05
1
3
5.0033
CMC Na 0,5%
3
5.1900
EEDS 2000 mg/kg bb
3
5.7633
Sig.
.431
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
68
Universitas Sumatera Utara
Lampiran 9. (lanjutan)
b.
Berat organ retatif ginjal kanan
Tests of Normality
Kolmogorov-Smirnova
Kelompok
Statistic
Berat organ relatif CMC Na 0,5%
.333
ginjal kanan
EEDS 2000
.314
mg/kg bb
EEDS 5000
.292
mg/kg bb
a. Lilliefors Significance Correction
df
Shapiro-Wilk
Sig. Statistic
3
.
.862
df
Sig.
3 .274
3
.
.893
3
.363
3
.
.923
3
.463
Descriptives
Berat organ relatif ginjal kanan
CMC Na
0,5%
EEDS 2000
mg/kg bb
95% Confidence
Interval for
Mean
Std.
Std. Lower Upper
N Mean Deviation Error Bound Bound Minimum Maximum
3 .6000 .07000 .04041 .4261
.7739
.52
.65
3 .5700
.02646 .01528
.5043
.6357
.54
.59
EEDS 5000
mg/kg bb
3 .5633
.02082 .01202
.5116
.6150
.54
.58
Total
9 .5778
.04236 .01412
.5452
.6103
.52
.65
.569
Sig.
.594
ANOVA
Berat organ relatif ginjal kanan
Between Groups
Sum of
Squares
.002
df
Mean Square
2
.001
Within Groups
.012
6
Total
.014
8
F
.002
69
Universitas Sumatera Utara
Lampiran 9. (lanjutan)
Multiple Comparisons
Berat organ relatif ginjal kanan
Tukey HSD
(I) Kelompok (J) Kelompok
CMC Na 0,5% EEDS 2000
mg/kg bb
EEDS 5000
mg/kg bb
CMC Na 0,5%
EEDS 2000
mg/kg bb
EEDS 5000
mg/kg bb
CMC Na 0,5%
EEDS 5000
mg/kg bb
EEDS 2000
mg/kg bb
Mean
Difference Std.
(I-J)
Error Sig.
.03000 .03662
.706
95% Confidence
Interval
Lower
Upper
Bound
Bound
-.0823
.1423
.03667 .03662
.603
-.0757
.1490
-.03000 .03662
.706
-.1423
.0823
.00667 .03662
.982
-.1057
.1190
-.03667 .03662
.603
-.1490
.0757
-.00667 .03662
.982
-.1190
.1057
Berat organ relatif ginjal kanan
Tukey HSD
a
Subset for alpha = 0.05
Kelompok
N
1
EEDS 5000 mg/kg bb
3
.5633
EEDS 2000 mg/kg bb
3
.5700
CMC Na 0,5%
3
.6000
Sig.
.603
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
70
Universitas Sumatera Utara
Lampiran 9. (lanjutan)
c.
Berat organ relatif ginjal kiri
Tests of Normality
Kolmogorov-Smirnova
Kelompok
Statistic
Berat organ relatif CMC Na 0,5%
.216
ginjal kiri
EEDS 2000
.304
mg/kg bb
EEDS 5000
.292
mg/kg bb
a. Lilliefors Significance Correction
df
Shapiro-Wilk
Sig. Statistic
3
.
.989
df
Sig.
3 .797
3
.
.907
3
.407
3
.
.923
3
.463
Descriptives
Berat organ relatif ginjal kiri
CMC Na
0,5%
EEDS 2000
mg/kg bb
95% Confidence
Interval for
Mean
Std.
Std. Lower Upper
N Mean Deviation Error Bound Bound Minimum Maximum
3 .5767 .13577 .07839 .2394
.9139
.45
.72
3 .5933
.04726 .02728
.4759
.7107
.54
.63
EEDS 5000
mg/kg bb
3 .5567
.02082 .01202
.5050
.6084
.54
.58
Total
9 .5756
.07435 .02478
.5184
.6327
.45
.72
.144
Sig.
.869
ANOVA
Berat organ relatif ginjal kiri
Between Groups
Sum of
Squares
.002
df
Mean Square
2
.001
Within Groups
.042
6
Total
.044
8
F
.007
71
Universitas Sumatera Utara
Lampiran 9. (lanjutan)
Multiple Comparisons
Berat organ relatif ginjal kiri
Tukey HSD
(I) Kelompok (J) Kelompok
CMC Na 0,5% EEDS 2000
mg/kg bb
EEDS 2000
mg/kg bb
EEDS 5000
mg/kg bb
Mean
Difference Std.
(I-J)
Error Sig.
-.01667 .06848
.968
95% Confidence
Interval
Lower
Upper
Bound
Bound
-.2268
.1934
EEDS 5000
mg/kg bb
CMC Na 0,5%
.02000 .06848
.954
-.1901
.2301
.01667 .06848
.968
-.1934
.2268
EEDS 5000
mg/kg bb
CMC Na 0,5%
.03667 .06848
.857
-.1734
.2468
-.02000 .06848
.954
-.2301
.1901
-.03667 .06848
.857
-.2468
.1734
EEDS 2000
mg/kg bb
Berat organ relatif ginjal kiri
Tukey HSD
a
Subset for alpha = 0.05
Kelompok
N
1
EEDS 5000 mg/kg bb
3
.5567
CMC Na 0,5%
3
.5767
EEDS 2000 mg/kg bb
3
.5933
Sig.
.857
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3.000.
72
Universitas Sumatera Utara