Pengaruh Interesterifikasi pada Lemak Sapi dan Minyak Kelapa Sawit terhadap Profil Lipida Marmut
Lampiran 1. Bagan Alur Prosedur Interesterifikasi Kimia
150 ml sampel
Ditambah 15 ml NaOCH3 0,1N
Diaduk dengan kecepatan 4000 rpm
pada suhu 60-70oC selama variasi
waktu (30,60,90,120 menit)
Hasil reaksi
Ditambah asam Sitrat 20% (b/v)
Dimasukkan dalam corong pisah
Ditambah 150 ml n-heksan
Dicuci dengan akuades 3 (tiga) kali
Lapisan atas
Lapisan bawah
Ditambah Na2SO4 anhidrat
Disaring
dirotarievaporasi
Ditentukan titik leburnya
Larutan siap diuji
pada marmut
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Universitas Sumatera Utara
Lampiran 2. Bagan Alur Penentuan Titik Lebur
Sampel cair
Dimasukkan dalam pipa kapiler Ø 1 mm
Dibekukan selama 24 jam dalam
freezer
Dimasukkan dalam alat Melting
Point Aparatus
Dinaikkan suhu pada alat secara
bertahap & perlahan hingga meleleh
Dicatat titik leburnya
Titik lebur sampel
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Universitas Sumatera Utara
Lampiran 3. Bagan Alur Pengujian Efek terhadap Profil Lipida Serum Darah
Marmut
Marmut
Dikondisikan selama 2 minggu
Diukur serum
d h
Diberi perlakuan selama
21 hari
Kadar Profil
Lipida Awal
Diukur kadar profil
lipida pada hari ke-1,7
dan 14
Kadar Profil
Lipida
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Universitas Sumatera Utara
Lampiran 4. Bagan Alur Pengambilan Serum Darah Marmut
Marmut
Dipuasakan selama 10-14 jam
Dipangkas bulu kakinya
Disikat kuku kakinya dengan
sikat gigi basah
Kuku yang bersih
Dibilas dengan alkohol
Dipotong dengan pemotong
kuku sampai berdarah
Darah marmut
Didiamkan selama 10 menit
Disentrifugasi dengan kecepatan
3000 rpm selama 15 menit
Diambil cairan bening
Serum siap diukur
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Universitas Sumatera Utara
Lampiran 5. Bagan Alur Pengukuran Kadar Kolesterol
Serum
Dipipet sebanyak 10 µl
Dimasukkan dalam tabung reaksi
yang berisi larutan reagensia
kolesterol sebanyak 1 ml
Dihomogenkan
Diinkubasi pada suhu kamar
selama 20 menit
Diukur pada mikrolab 300 dengan
panjang gelombang 546 nm
Dicatat hasilnya
Kadar kolesterol total
lxxvii
Universitas Sumatera Utara
Lampiran 6. Bagan Alur Pengukuran Kadar Trigliserida
Serum
Dipipet sebanyak 10 µl
Dimasukkan dalam tabung reaksi
yang berisi larutan reagensia
trigliserida sebanyak 1 ml
Dihomogenkan
Diinkubasi pada suhu kamar
selama 20 menit
Diukur pada mikrolab 300 dengan
panjang gelombang 546 nm
Dicatat hasilnya
Kadar trigliserida
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Universitas Sumatera Utara
Lampiran 7. Bagan Alur Pengukuran Kadar HDL
Serum
Dipipet sebanyak 100 µl
Dimasukkan dalam tabung reaksi
yang berisi larutan reagensia
kolesterol HDL sebanyak 1 ml
Dihomogenkan
Diinkubasi pada suhu kamar
selama 20 menit
Diukur pada mikrolab 300 dengan
panjang gelombang 546 nm
Dicatat hasilnya
Kadar HDL
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Universitas Sumatera Utara
Lampiran 8. Data Kadar Kolesterol Total Serum Darah Marmut (mg/dl)
Perlakuan
A
B
C
D
E
F
G
H
I
J
Kadar Kolesterol (mg/dl)
Berat
Badan
(gram)
Awal
1 hari
7 hari
14 hari
21 hari
256
258
240
236
222
208
204
226
213
254
249
238
227
244
208
266
218
208
257
268
261
265
299
275
211
214
207
241
232
217
34
36
39
48
38
45
41
47
46
43
40
46
48
45
42
49
45
42
47
46
42
45
41
46
34
40
42
45
39
41
51
55
53
62
65
60
81
88
82
82
84
88
84
87
85
51
47
45
55
54
50
76
72
75
72
79
75
70
72
75
71
73
75
77
73
76
96
93
95
99
92
95
97
93
91
53
48
46
58
59
57
51
56
54
56
60
58
61
64
66
76
70
74
74
69
73
63
60
61
64
60
61
66
63
59
48
43
41
46
48
40
42
48
46
44
42
45
43
49
42
78
72
77
68
65
66
60
58
58
61
57
58
63
59
54
46
42
40
43
44
51
40
46
43
42
41
43
40
47
40
lxxx
Universitas Sumatera Utara
Lampiran 9. Data Kadar Trigliserida Serum Darah Marmut (mg/dl)
Berat
Perlakuan Badan
(gram)
A
256
B
C
D
E
F
G
H
I
J
258
240
236
222
208
204
226
213
254
249
238
227
244
208
266
218
208
257
268
261
265
299
275
211
214
207
241
232
217
Kadar Trigliserida (mg/dl)
Awal
1 hari
7 hari
14 hari
21 hari
38
40
43
52
42
49
45
51
50
47
44
50
52
49
46
53
49
46
51
50
46
49
45
50
38
44
46
49
43
45
53
57
55
64
67
62
83
90
84
84
86
90
86
89
87
53
49
47
57
56
52
78
74
77
74
81
77
72
74
77
75
77
79
81
77
80
100
97
99
103
96
99
101
97
95
57
52
50
62
63
61
55
60
58
60
64
62
65
68
70
73
67
71
71
66
70
60
57
58
61
57
58
63
60
56
45
40
38
43
45
37
39
45
43
41
39
42
40
46
39
74
68
73
64
61
62
56
54
54
57
53
54
59
55
50
42
38
36
39
40
47
36
42
39
38
37
39
36
43
36
lxxxi
Universitas Sumatera Utara
Lampiran 10. Data Kadar HDL Serum Darah Marmut (mg/dl)
Perlakuan
A
B
C
D
E
F
G
H
I
J
Kadar HDL (mg/dl)
Berat
Badan
(gram)
Awal
1 hari
7 hari
14 hari
21 hari
256
258
240
236
222
208
226
204
213
254
249
238
227
244
208
266
218
208
257
268
261
265
299
275
211
214
207
241
232
217
11
10
15
16
17
14
11
19
14
15
13
18
13
12
14
16
19
17
12
17
16
14
17
12
14
13
17
15
16
13
11
9
15
16
18
14
11
18
13
15
12
18
14
12
13
16
18
16
12
18
16
14
16
12
13
12
17
15
15
12
12
10
15
17
18
15
11
18
14
16
11
18
14
12
14
17
18
17
12
17
17
13
17
13
12
13
16
16
14
13
11
10
14
16
17
13
12
17
13
16
13
19
15
13
15
24
26
23
20
22
22
16
20
16
14
15
19
20
17
15
12
9
13
15
16
13
13
19
12
15
12
18
15
14
14
29
31
28
24
25
26
22
26
23
20
20
24
25
23
21
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Universitas Sumatera Utara
Lampiran 11. Data Kadar LDL Serum Darah Marmut
Perlakuan
A
B
C
D
E
F
G
H
I
J
Kadar LDL (mg/dl)
Berat
Badan
(gram)
Awal
1 hari
7 hari
14 hari
21 hari
256
250
240
236
222
208
226
204
213
254
249
238
227
244
208
266
218
208
257
268
261
265
299
275
211
214
207
241
232
217
15.4
18
15.4
21.6
12.6
21.2
21
17.8
22
18.6
18.2
18
24.6
23.2
18.8
22.4
16.2
15.8
24.8
19
16.8
21.2
15
24
12.4
18.2
15.8
20.2
14.4
19
29.4
34.6
27
33.2
33.6
33.6
53.4
52
52.2
50.2
54.8
52
52.8
57.2
54.6
24.4
19.2
19.6
31.6
24.8
23.6
46.4
41.2
47.6
44.2
50.8
42.6
40.6
42.2
47.6
44
47.6
44.2
43.8
39.6
45
65
55.6
61.2
62.4
61.8
57.2
62.8
61.6
58
24.6
19.6
19
33.6
29.4
27.8
27
27
29.4
32
34.2
29.6
32
36.4
39
50.4
46.6
45.8
43.8
38.8
46
39
31.6
36.4
35.8
35.6
30.4
38.4
38
32.8
15
9
10.4
17.4
17
10.6
18.2
19
21.4
21.8
19.2
17.6
15
22.8
19.2
51.2
49.4
49.4
40.2
36.8
40.6
35.8
28.2
35.2
34.6
34.4
29.2
36.2
34
30
8.6
3.4
4.8
11.2
11
15.6
10.8
11.6
12.2
14.4
13.6
11.2
7.8
15.4
11.8
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Universitas Sumatera Utara
Lampiran 12. Analisis Statistik Kolesterol Total Pemberian Lemak Sapi
Univariate Analysis of Variance
Dependent Variable:Hasil Pengamatan
Lama
Lama
Reaksi Percoba
(menit) an
0
30
60
90
120
Total
Mean
Std. Deviation
N
0
36.3333
2.51661
3
1
53.0000
2.00000
3
7
73.0000
2.00000
3
14
73.3333
3.05505
3
21
75.6667
3.21455
3
Total
62.2667
16.02439
15
0
43.6667
5.13160
3
1
62.3333
2.51661
3
7
75.3333
2.08167
3
14
72.0000
2.64575
3
21
66.3333
1.52753
3
Total
63.9333
11.75625
15
0
44.6667
3.21455
3
1
83.6667
3.78594
3
7
94.6667
1.52753
3
14
61.3333
1.52753
3
21
58.6667
1.15470
3
Total
68.6000
18.81033
15
0
43.0000
3.00000
3
1
84.6667
3.05505
3
7
95.3333
3.51188
3
14
61.6667
2.08167
3
21
58.6667
2.08167
3
Total
68.6667
19.64567
15
0
45.0000
3.00000
3
1
85.3333
1.52753
3
7
93.6667
3.05505
3
14
62.6667
3.51188
3
21
58.6667
4.50925
3
Total
69.0667
18.71007
15
0
42.5333
4.42181
15
1
73.8000
14.16838
15
7
86.4000
10.60189
15
14
66.2000
5.94258
15
21
63.6000
7.34652
15
Total
66.5067
17.00189
75
lxxxiv
Universitas Sumatera Utara
Lanjutan Lampiran 12.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Pengamatan
Type III
Sum of
Squares
Source
Corrected
Model
Intercept
20983.413a
Menit_Ke
603.013
Hari_Ke
331735.25
3
15483.013
Menit_Ke *
Hari_Ke
Error
Total
Mean
Square
df
F
Sig.
107.32
1
331735.25 4.072E
1
3
4
24
874.309
4
16
407.333
353126.00
0
50
Observed
Powerb
.000
.981
2575.705
1.000
.000
.999 40720.367
1.000
150.753 18.505
.000
.597
74.020
1.000
475.13
3
.000
.974
1900.534
1.000
306.087 37.572
.000
.923
601.152
1.000
4 3870.753
4897.387
Partial Eta Noncent.
Squared Parameter
8.147
75
Corrected
21390.747
74
Total
a. R Squared = .981 (Adjusted R Squared =
.972)
Post Hoc Tests
Lama Reaksi (menit)
Multiple Comparisons
Hasil Pengamatan
Tukey HSD
(I)
Lama
Reaksi
(menit)
(J)
Lama
Reaksi
(menit)
0
30
-1.6667
1.04222
.505
-4.6159
1.2826
60
*
1.04222
.000
-9.2826
-3.3841
*
1.04222
.000
-9.3493
-3.4507
*
-6.8000
1.04222
.000
-9.7493
-3.8507
1.6667
1.04222
.505
-1.2826
4.6159
*
1.04222
.000
-7.6159
-1.7174
*
1.04222
.000
-7.6826
-1.7841
*
1.04222
.000
-8.0826
-2.1841
*
1.04222
.000
3.3841
9.2826
*
1.04222
.000
1.7174
7.6159
90
-.0667
1.04222
1.000
-3.0159
2.8826
120
-.4667
1.04222
.991
-3.4159
2.4826
90
120
30
0
60
90
120
60
0
30
95% Confidence Interval
Mean Difference
(I-J)
-6.3333
-6.4000
-4.6667
-4.7333
-5.1333
6.3333
4.6667
Std. Error
Sig.
Lower Bound
Upper Bound
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Universitas Sumatera Utara
Lanjutan Lampiran 12
90
6.4000*
1.04222
*
1.04222
60
.0667
1.04222
120
-.4000
1.04222
*
*
.4667
0
30
120
4.7333
0
6.8000
30
5.1333
60
3.4507
9.3493
.000
1.7841
7.6826
1.000
-2.8826
3.0159
.995
-3.3493
2.5493
1.04222
.000
3.8507
9.7493
1.04222
.000
2.1841
8.0826
1.04222
.991
-2.4826
3.4159
.995
-2.5493
3.3493
90
.4000
1.04222
Based on observed means.
The error term is Mean Square(Error) = 8.147.
*. The mean difference is significant at the .05 level.
.000
Homogeneous Subsets
Hasil Pengamatan
Tukey HSD
Lama
Reaksi
(menit)
Subset
N
1
2
0
15
62.2667
30
15
63.9333
60
15
68.6000
90
15
68.6667
120
15
69.0667
Sig.
.505
.991
Means for groups in homogeneous subsets are
displayed.
Based on observed means.
The error term is Mean Square(Error) = 8.147.
lxxxvi
Universitas Sumatera Utara
Lampiran 13. Analisis Statistik Trigliserida Pemberian Lemak Sapi
Univariate Analysis of Variance
Dependent Variable:Hasil Pengamatan
Lama
Reaksi
(menit) Lama Percobaan
0
30
60
90
120
Total
Mean
Std. Deviation
N
0
40.3333
2.51661
3
1
55.0000
2.00000
3
7
77.0000
2.00000
3
14
70.3333
3.05505
3
21
71.6667
3.21455
3
Total
62.8667
14.08072
15
0
47.6667
5.13160
3
1
64.3333
2.51661
3
7
79.3333
2.08167
3
14
69.0000
2.64575
3
21
62.3333
1.52753
3
Total
64.5333
10.94706
15
0
48.6667
3.21455
3
1
85.6667
3.78594
3
7
98.6667
1.52753
3
14
58.3333
1.52753
3
21
54.6667
1.15470
3
Total
69.2000
20.23505
15
0
47.0000
3.00000
3
1
86.6667
3.05505
3
7
99.3333
3.51188
3
14
58.6667
2.08167
3
21
54.6667
2.08167
3
Total
69.2667
20.98117
15
0
49.0000
3.00000
3
1
87.3333
1.52753
3
7
97.6667
3.05505
3
14
59.6667
3.51188
3
21
54.6667
4.50925
3
Total
69.6667
20.09146
15
0
46.5333
4.42181
15
1
75.8000
14.16838
15
7
90.4000
10.60189
15
14
63.2000
5.94258
15
21
59.6000
7.34652
15
Total
67.1067
17.47707
75
lxxxvii
Universitas Sumatera Utara
Lanjutan Lampiran 13.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Pengamatan
Type III Sum
of Squares df
Source
Corrected
Model
Intercept
Menit_Ke
Hari_Ke
Menit_Ke *
Hari_Ke
Error
Total
Corrected Total
22195.813a
337747.853
Mean
Square
24
F
Sig.
924.826 113.522 .000
1 337747.853
4.146E
.000
4
Partial Eta Noncent.
Squared Parameter
.982
Observed
Powerb
2724.527
1.000
.999 41458.411
1.000
603.013
4
150.753 18.505 .000
.597
74.020
1.000
16695.413
4
4173.853 512.339 .000
.976
2049.355
1.000
4897.387
16
306.087 37.572 .000
.923
601.152
1.000
407.333
50
360351.000
75
22603.147
74
8.147
Post Hoc Tests
Lama Reaksi (menit)
Multiple Comparisons
Hasil Pengamatan
Tukey HSD
(I)
Lama
Reaksi
(menit)
(J)
Lama
Reaksi
(menit)
0
30
-1.6667
1.04222
.505
-4.6159
1.2826
60
*
1.04222
.000
-9.2826
-3.3841
*
1.04222
.000
-9.3493
-3.4507
*
-6.8000
1.04222
.000
-9.7493
-3.8507
1.6667
1.04222
.505
-1.2826
4.6159
*
1.04222
.000
-7.6159
-1.7174
*
1.04222
.000
-7.6826
-1.7841
*
1.04222
.000
-8.0826
-2.1841
0
6.3333
*
1.04222
.000
3.3841
9.2826
30
4.6667*
1.04222
.000
1.7174
7.6159
90
-.0667
1.04222
1.000
-3.0159
2.8826
120
-.4667
1.04222
.991
-3.4159
2.4826
90
120
30
0
60
90
120
60
95% Confidence Interval
Mean Difference
(I-J)
-6.3333
-6.4000
-4.6667
-4.7333
-5.1333
Std. Error
Sig.
Lower Bound
Upper Bound
lxxxviii
Universitas Sumatera Utara
Lanjutan Lampiran 13.
90
120
0
6.4000*
1.04222
.000
3.4507
9.3493
30
4.7333*
1.04222
.000
1.7841
7.6826
60
.0667
1.04222
1.000
-2.8826
3.0159
120
-.4000
1.04222
.995
-3.3493
2.5493
6.8000
*
1.04222
.000
3.8507
9.7493
5.1333
*
1.04222
.000
2.1841
8.0826
.4667
1.04222
.991
-2.4826
3.4159
.995
-2.5493
3.3493
0
30
60
90
.4000
1.04222
Based on observed means.
The error term is Mean Square(Error) = 8.147.
*. The mean difference is significant at the .05 level.
Homogeneous Subsets
Hasil Pengamatan
Tukey HSD
Lama
Reaksi
(menit)
0
30
60
90
120
Sig.
Subset
N
1
15
15
15
15
15
2
62.8667
64.5333
.505
69.2000
69.2667
69.6667
.991
Means for groups in homogeneous subsets are
displayed.
Based on observed means.
The error term is Mean Square(Error) = 8.147.
lxxxix
Universitas Sumatera Utara
Lampiran 14. Analisis Statistik HDL Pemberian Lemak Sapi
Univariate Analysis of Variance
Descriptive Statistics
Dependent Variable:Hasil
Lama
Reaksi Hari_K
(Menit) e
Mean
0
0
12.0000
2.64575
3
1
11.6667
3.05505
3
7
12.3333
2.51661
3
14
11.6667
2.08167
3
21
11.3333
2.08167
3
Total
11.8000
2.14476
15
0
15.6667
1.52753
3
1
16.0000
2.00000
3
7
16.6667
1.52753
3
14
15.3333
2.08167
3
21
14.6667
1.52753
3
Total
15.6667
1.63299
15
0
14.6667
4.04145
3
1
14.0000
3.60555
3
7
14.3333
3.51188
3
14
14.0000
2.64575
3
21
14.6667
3.78594
3
Total
14.3333
3.01583
15
0
15.3333
2.51661
3
1
15.0000
3.00000
3
7
15.0000
3.60555
3
14
16.0000
3.00000
3
21
15.0000
3.00000
3
Total
30
60
90
120
Total
Std. Deviation
N
15.2667
2.60403
15
0
13.0000
1.00000
3
1
13.0000
1.00000
3
7
13.3333
1.15470
3
14
14.3333
1.15470
3
21
14.3333
.57735
3
Total
13.6000
1.05560
15
0
14.1333
2.61498
15
1
13.9333
2.76371
15
7
14.3333
2.71679
15
14
14.2667
2.46306
15
21
14.0000
2.50713
15
Total
14.1333
2.54863
75
xc
Universitas Sumatera Utara
Lanjutan Lampiran 14.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Type III Sum
Source
of Squares
Partial Eta
df
Mean Square
F
Sig.
Squared
158.667a
24
6.611
1.027
.454
.330
14981.333
1
14981.333
2.326E3
.000
.979
141.067
4
35.267
5.476
.001
.305
1.733
4
.433
.067
.991
.005
15.867
16
.992
.154
1.000
.047
Error
322.000
50
6.440
Total
15462.000
75
480.667
74
Corrected Model
Intercept
Menit_Ke
Hari_Ke
Menit_Ke *
Hari_Ke
Corrected Total
a. R Squared = .330 (Adjusted R Squared = .009)
Post Hoc Tests
Lama Reaksi (Menit)
Multiple Comparisons
Hasil
Tukey HSD
(I)
Lama
Reaksi
(Menit)
0
(J)
Lama
Reaksi Mean Difference
(Menit)
(I-J)
Std. Error
60
Sig.
Lower Bound
Upper Bound
*
.92664
.001
-6.4889
-1.2444
60
-2.5333
.92664
.063
-5.1556
.0889
90
*
.92664
.004
-6.0889
-.8444
-1.8000
.92664
.309
-4.4222
.8222
*
.92664
.001
1.2444
6.4889
60
1.3333
.92664
.606
-1.2889
3.9556
90
.4000
.92664
.993
-2.2222
3.0222
120
2.0667
.92664
.186
-.5556
4.6889
0
2.5333
.92664
.063
-.0889
5.1556
30
-1.3333
.92664
.606
-3.9556
1.2889
90
-.9333
.92664
.851
-3.5556
1.6889
120
.7333
.92664
.932
-1.8889
3.3556
30
120
30
95% Confidence Interval
0
-3.8667
-3.4667
3.8667
xci
Universitas Sumatera Utara
Lanjutan Lampiran 14.
90
120
0
3.4667*
.92664
.004
.8444
6.0889
30
-.4000
.92664
.993
-3.0222
2.2222
60
.9333
.92664
.851
-1.6889
3.5556
120
1.6667
.92664
.386
-.9556
4.2889
0
1.8000
.92664
.309
-.8222
4.4222
30
-2.0667
.92664
.186
-4.6889
.5556
60
-.7333
.92664
.932
-3.3556
1.8889
.386
-4.2889
.9556
90
-1.6667
.92664
Based on observed means.
The error term is Mean Square(Error) = 6.440.
*. The mean difference is significant at the .05 level.
Homogeneous Subsets
Hasil
Tukey HSD
Lama
Reaksi
(Menit)
Subset
N
1
2
0
15
11.8000
120
15
13.6000
13.6000
60
15
14.3333
14.3333
90
15
15.2667
30
15
15.6667
Sig.
.063
.186
Means for groups in homogeneous subsets
are displayed.
Based on observed means.
The error term is Mean Square(Error) =
6.440.
xcii
Universitas Sumatera Utara
Lampiran 15. Analisis Statistik LDL Pemberian Lemak Sapi
Univariate Analysis of Variance
Descriptive Statistics
Dependent Variable:Hasil
Lama
Reaksi Hari_K
(Menit) e
Mean
0
0
16.2667
1.50111
3
1
30.3333
3.88501
3
7
45.2667
2.02320
3
14
47.6000
2.45764
3
21
50.0000
1.03923
3
Total
37.8933
13.40892
15
0
18.4667
5.08462
3
1
33.4667
.23094
3
7
42.8000
2.83549
3
14
42.8667
3.68963
3
21
39.2000
2.08806
3
Total
35.3600
9.82015
15
0
20.2667
2.19393
3
1
52.5333
.75719
3
7
60.6000
4.72864
3
14
35.6667
3.75411
3
21
33.0667
4.22532
3
Total
40.4267
15.18869
15
0
18.2667
.30551
3
1
52.3333
2.31805
3
7
60.4667
2.84488
3
14
33.9333
3.06159
3
21
32.7333
3.06159
3
Total
39.5467
15.72159
15
0
22.2000
3.02655
3
1
54.8667
2.21209
3
7
60.8000
2.49800
3
14
36.4000
3.12410
3
21
33.4000
3.14325
3
Total
41.5333
14.94192
15
0
19.0933
3.21414
15
1
44.7067
11.07993
15
7
53.9867
8.85453
15
14
39.2933
5.98707
15
21
37.6800
7.26825
15
Total
38.9520
13.76996
75
30
60
90
120
Total
Std. Deviation
N
xciii
Universitas Sumatera Utara
Lanjutan Lampiran 15.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Type III Sum
of Squares
Source
df
Mean Square
F
Sig.
Partial Eta
Squared
Corrected Model
13606.441a
24
566.935
66.725
.000
.970
Intercept
113794.373
1
113794.373
1.339E4
.000
.996
348.222
4
87.055
10.246
.000
.450
9828.878
4
2457.219
289.203
.000
.959
3429.341
16
214.334
25.226
.000
.890
424.827
50
8.497
127825.640
75
14031.267
74
Menit_Ke
Hari_Ke
Menit_Ke *
Hari_Ke
Error
Total
Corrected Total
a. R Squared = .970 (Adjusted R Squared = .955)
Post Hoc Tests
Lama Reaksi (Menit)
Multiple Comparisons
Hasil
Tukey HSD
(I)
Lama
Reaksi
(Menit)
(J)
Lama
Reaksi
(Menit)
0
30
2.5333
1.06436
.138
-.4786
5.5453
60
-2.5333
1.06436
.138
-5.5453
.4786
90
-1.6533
1.06436
.534
-4.6653
1.3586
120
*
-3.6400
1.06436
.011
-6.6519
-.6281
0
-2.5333
1.06436
.138
-5.5453
.4786
*
1.06436
.000
-8.0786
-2.0547
*
1.06436
.002
-7.1986
-1.1747
*
-6.1733
1.06436
.000
-9.1853
-3.1614
2.5333
1.06436
.138
-.4786
5.5453
*
5.0667
1.06436
.000
2.0547
8.0786
30
-5.0667
90
-4.1867
0
30
90
Std. Error
Sig.
Lower Bound
Upper Bound
.8800
1.06436
.921
-2.1319
3.8919
-1.1067
1.06436
.836
-4.1186
1.9053
0
1.6533
1.06436
.534
-1.3586
4.6653
30
4.1867
*
1.06436
.002
1.1747
7.1986
60
-.8800
1.06436
.921
-3.8919
2.1319
120
-1.9867
1.06436
.348
-4.9986
1.0253
120
90
Mean Difference
(I-J)
60
120
60
95% Confidence Interval
xciv
Universitas Sumatera Utara
Lanjutan Lampiran 15.
120
*
1.06436
.011
.6281
6.6519
30
*
6.1733
1.06436
.000
3.1614
9.1853
60
1.1067
1.06436
.836
-1.9053
4.1186
90
1.9867
1.06436
.348
-1.0253
4.9986
0
3.6400
Based on observed means.
The error term is Mean Square(Error) = 8.497.
*. The mean difference is significant at the .05 level.
Homogeneous Subsets
Hasil
Tukey HSD
Lama
Reaksi
(Menit)
Subset
N
1
2
3
30
15
35.3600
0
15
37.8933
37.8933
90
15
39.5467
39.5467
60
15
40.4267
40.4267
120
15
41.5333
Sig.
.138
.138
.348
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 8.497.
xcv
Universitas Sumatera Utara
Lampiran 16. Analisis Statistik Kolesterol Pemberian Minyak Kelapa Sawit
Univariate Analysis of Variance
Descriptive Statistics
Dependent Variable:Hasil Pengamatan
Lama
Lama
Reaksi Percoba
(menit) an
0
30
60
90
120
Total
Mean
Std. Deviation
N
0
45.3333
3.51188
3
1
47.6667
3.05505
3
7
49.0000
3.60555
3
14
44.0000
3.60555
3
21
42.6667
3.05505
3
Total
45.7333
3.73146
15
0
45.0000
2.64575
3
1
53.0000
2.64575
3
7
58.0000
1.00000
3
14
44.6667
4.16333
3
21
46.0000
4.35890
3
Total
49.3333
6.11400
15
0
44.0000
2.64575
3
1
74.3333
2.08167
3
7
53.6667
2.51661
3
14
45.3333
3.05505
3
21
43.0000
3.00000
3
Total
52.0667
12.37778
15
0
38.6667
4.16333
3
1
75.3333
3.51188
3
7
58.0000
2.00000
3
14
43.6667
1.52753
3
21
42.0000
1.00000
3
Total
51.5333
14.27719
15
0
41.6667
3.05505
3
1
72.3333
2.51661
3
7
63.6667
2.51661
3
14
44.6667
3.78594
3
21
42.3333
4.04145
3
Total
52.9333
13.37090
15
0
42.9333
3.76955
15
1
64.5333
12.39739
15
7
56.4667
5.48852
15
14
44.4667
2.89992
15
21
43.2000
3.16679
15
Total
50.3200
10.85551
75
xcvi
Universitas Sumatera Utara
Lanjutan Lampiran 16.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Pengamatan
Type III
Sum of
Squares
Source
Corrected
Model
Intercept
F
Sig.
Partial Eta Noncent.
Squared Parameter
Observed
Powerb
8252.320a
24
343.847 36.736
.000
.946
881.658
1.000
189907.68
0
1
189907.68 2.029E
0
4
.000
.998 20289.282
1.000
500.453
4
125.113 13.367
.000
.517
53.467
1.000
151.97
1
.000
.924
607.883
1.000
128.880 13.769
.000
.815
220.308
1.000
Menit_Ke
Hari_Ke
5689.787
Menit_Ke *
Hari_Ke
Error
Total
Mean
Square
df
4 1422.447
2062.080
16
468.000
50
198628.00
0
75
9.360
Corrected
8720.320
74
Total
a. R Squared = .946 (Adjusted R Squared =
.921)
Post Hoc Tests
Lama Reaksi (menit)
Multiple Comparisons
Hasil Pengamatan
Tukey HSD
(I)
Lama
Reaksi
(menit)
0
(J)
Lama
Reaksi
(menit)
Std. Error
Sig.
Lower Bound
Upper Bound
1.11714
.018
-6.7613
-.4387
*
1.11714
.000
-9.4946
-3.1720
*
1.11714
.000
-8.9613
-2.6387
*
1.11714
.000
-10.3613
-4.0387
0
3.6000
*
1.11714
.018
.4387
6.7613
60
-2.7333
1.11714
.120
-5.8946
.4280
90
-2.2000
1.11714
.296
-5.3613
.9613
*
1.11714
.018
-6.7613
-.4387
*
1.11714
.000
3.1720
9.4946
30
2.7333
1.11714
.120
-.4280
5.8946
90
.5333
1.11714
.989
-2.6280
3.6946
120
-.8667
1.11714
.936
-4.0280
2.2946
30
90
120
120
60
Mean Difference
(I-J)
*
60
30
95% Confidence Interval
0
-3.6000
-6.3333
-5.8000
-7.2000
-3.6000
6.3333
xcvii
Universitas Sumatera Utara
Lanjutan Lampiran 16.
90
120
0
5.8000*
1.11714
.000
30
2.2000
1.11714
60
-.5333
1.11714
120
-1.4000
7.2000
*
3.6000
*
.8667
0
30
60
2.6387
8.9613
.296
-.9613
5.3613
.989
-3.6946
2.6280
1.11714
.720
-4.5613
1.7613
1.11714
.000
4.0387
10.3613
1.11714
.018
.4387
6.7613
1.11714
.936
-2.2946
4.0280
.720
-1.7613
4.5613
90
1.4000
1.11714
Based on observed means.
The error term is Mean Square(Error) = 9.360.
*. The mean difference is significant at the .05 level.
Homogeneous Subsets
Hasil Pengamatan
Tukey HSD
Lama
Reaksi
(menit)
Subset
N
1
2
3
0
15
45.7333
30
15
49.3333
90
15
51.5333
51.5333
60
15
52.0667
52.0667
120
15
52.9333
Sig.
1.000
.120
.720
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 9.360.
xcviii
Universitas Sumatera Utara
Lampiran 17. Analisis Statistik Trigliserida Pemberian Minyak Kelapa Sawit
Univariate Analysis of Variance
Descriptive Statistics
Dependent Variable:Hasil Pengamatan
Lama
Lama
Reaksi Percoba
(menit) an
0
30
60
90
120
Total
Mean
Std. Deviation
N
0
49.3333
3.51188
3
1
49.6667
3.05505
3
7
53.0000
3.60555
3
14
41.0000
3.60555
3
21
38.6667
3.05505
3
Total
46.3333
6.37704
15
0
49.0000
2.64575
3
1
55.0000
2.64575
3
7
62.0000
1.00000
3
14
41.6667
4.16333
3
21
42.0000
4.35890
3
Total
49.9333
8.50602
15
0
48.0000
2.64575
3
1
76.3333
2.08167
3
7
57.6667
2.51661
3
14
42.3333
3.05505
3
21
39.0000
3.00000
3
Total
52.6667
14.07463
15
0
42.6667
4.16333
3
1
77.3333
3.51188
3
7
62.0000
2.00000
3
14
40.6667
1.52753
3
21
38.0000
1.00000
3
Total
52.1333
15.88740
15
0
45.6667
3.05505
3
1
74.3333
2.51661
3
7
67.6667
2.51661
3
14
41.6667
3.78594
3
21
38.3333
4.04145
3
Total
53.5333
15.36167
15
0
46.9333
3.76955
15
1
66.5333
12.39739
15
7
60.4667
5.48852
15
14
41.4667
2.89992
15
21
39.2000
3.16679
15
Total
50.9200
12.57062
75
xcix
Universitas Sumatera Utara
Lanjutan Lampiran 17.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Pengamatan
Type III Sum
Source
of Squares
Corrected
Mean
df
Square
F
11225.520a
24
194463.480
1
500.453
4
125.113
8662.987
4
2062.080
Error
Total
Model
Intercept
Observed
Squared
Parameter
Powerb
1.000
.998 20776.013
1.000
13.367 .000
.517
53.467
1.000
2165.747
231.383 .000
.949
925.533
1.000
16
128.880
13.769 .000
.815
220.308
1.000
468.000
50
9.360
206157.000
75
11693.520
74
Hari_Ke
Menit_Ke
* Hari_Ke
Corrected
Total
194463.48
0
49.971 .000
Noncent.
1199.308
Menit_Ke
467.730
Sig.
Partial Eta
2.078E4 .000
.960
a. R Squared = .960 (Adjusted R Squared = .941)
b. Computed using alpha = .05
Post Hoc Tests
Lama Reaksi (menit)
Multiple Comparisons
Hasil Pengamatan
Tukey HSD
(J)
(I) Lama Lama
Reaksi Reaksi
(menit) (menit)
0
Mean Difference
(I-J)
Std. Error
Sig.
Lower Bound
Upper Bound
*
1.11714
.018
-6.7613
-.4387
*
1.11714
.000
-9.4946
-3.1720
*
1.11714
.000
-8.9613
-2.6387
*
1.11714
.000
-10.3613
-4.0387
0
3.6000
*
1.11714
.018
.4387
6.7613
60
-2.7333
1.11714
.120
-5.8946
.4280
90
-2.2000
1.11714
.296
-5.3613
.9613
*
1.11714
.018
-6.7613
-.4387
30
60
90
120
30
95% Confidence Interval
120
-3.6000
-6.3333
-5.8000
-7.2000
-3.6000
c
Universitas Sumatera Utara
Lanjutan Lampiran 17.
60
90
120
0
6.3333*
1.11714
.000
30
2.7333
1.11714
90
.5333
1.11714
120
-.8667
*
30
3.1720
9.4946
.120
-.4280
5.8946
.989
-2.6280
3.6946
1.11714
.936
-4.0280
2.2946
1.11714
.000
2.6387
8.9613
2.2000
1.11714
.296
-.9613
5.3613
60
-.5333
1.11714
.989
-3.6946
2.6280
120
-1.4000
1.11714
.720
-4.5613
1.7613
*
1.11714
.000
4.0387
10.3613
*
1.11714
.018
.4387
6.7613
.8667
1.11714
.936
-2.2946
4.0280
.720
-1.7613
4.5613
0
5.8000
0
7.2000
30
3.6000
60
90
1.4000
1.11714
Based on observed means.
The error term is Mean Square(Error) = 9.360.
*. The mean difference is significant at the .05 level.
Homogeneous Subsets
Hasil Pengamatan
Tukey HSD
Lama
Reaksi
(menit)
Subset
N
1
2
3
0
15
46.3333
30
15
49.9333
90
15
52.1333
52.1333
60
15
52.6667
52.6667
120
15
53.5333
Sig.
1.000
.120
.720
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 9.360.
ci
Universitas Sumatera Utara
Lampiran 18. Analisis Statistik HDL Pemberian Minyak Kelapa Sawit
Univariate Analysis of Variance
Dependent Variable:Hasil
Lama
Reaksi Hari_K
(Menit) e
Mean
0
0
17.3333
1.52753
3
1
16.6667
1.15470
3
7
17.3333
.57735
3
14
24.3333
1.52753
3
21
29.3333
1.52753
3
Total
21.0000
5.31843
15
0
15.0000
2.64575
3
1
15.3333
3.05505
3
7
15.3333
2.88675
3
14
21.3333
1.15470
3
21
25.0000
1.00000
3
Total
18.4000
4.64143
15
0
14.3333
2.51661
3
1
14.0000
2.00000
3
7
14.3333
2.30940
3
14
17.3333
2.30940
3
21
23.6667
2.08167
3
Total
16.7333
4.25049
15
0
14.6667
2.08167
3
1
14.0000
2.64575
3
7
13.6667
2.08167
3
14
16.0000
2.64575
3
21
21.3333
2.30940
3
Total
15.9333
3.53486
15
0
14.6667
1.52753
3
1
14.0000
1.73205
3
7
14.3333
1.52753
3
14
17.3333
2.51661
3
21
23.0000
2.00000
3
Total
16.6667
3.84831
15
0
15.2000
2.11119
15
1
14.8000
2.17781
15
7
15.0000
2.17124
15
14
19.2667
3.67359
15
21
24.4667
3.20416
15
Total
17.7467
4.62103
75
30
60
90
120
Total
Std. Deviation
N
cii
Universitas Sumatera Utara
Lanjutan Lampiran 18.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Type III Sum
Source
of Squares
Partial Eta
df
Mean Square
F
Sig.
Squared
Corrected Model
1366.187a
24
56.924
13.300
.000
.865
Intercept
23620.813
1
23620.813
5.519E3
.000
.991
247.387
4
61.847
14.450
.000
.536
1052.720
4
263.180
61.491
.000
.831
66.080
16
4.130
.965
.507
.236
Error
214.000
50
4.280
Total
25201.000
75
1580.187
74
Menit_Ke
Hari_Ke
Menit_Ke *
Hari_Ke
Corrected Total
a. R Squared = .865 (Adjusted R Squared = .800)
Post Hoc Tests
Lama Reaksi (Menit)
Multiple Comparisons
Hasil
Tukey HSD
(I)
Lama
Reaksi
(Menit)
0
(J)
Lama
Reaksi Mean Difference
(Menit)
(I-J)
Std. Error
Upper Bound
.75542
.010
.4623
4.7377
.75542
.000
2.1290
6.4044
*
.75542
.000
2.9290
7.2044
*
.75542
.000
2.1956
6.4710
*
-2.6000
.75542
.010
-4.7377
-.4623
60
1.6667
.75542
.194
-.4710
3.8044
90
*
.75542
.016
.3290
4.6044
1.7333
.75542
.164
-.4044
3.8710
*
.75542
.000
-6.4044
-2.1290
30
-1.6667
.75542
.194
-3.8044
.4710
90
.8000
.75542
.826
-1.3377
2.9377
120
.0667
.75542
1.000
-2.0710
2.2044
*
.75542
.000
-7.2044
-2.9290
*
.75542
.016
-4.6044
-.3290
60
-.8000
.75542
.826
-2.9377
1.3377
120
-.7333
.75542
.867
-2.8710
1.4044
120
0
120
90
Lower Bound
*
30
90
60
Sig.
*
60
30
95% Confidence Interval
0
0
30
2.6000
4.2667
5.0667
4.3333
2.4667
-4.2667
-5.0667
-2.4667
ciii
Universitas Sumatera Utara
Lanjutan Lampiran 18.
120
0
-4.3333*
.75542
30
-1.7333
60
-.0667
.000
-6.4710
.75542
.164
-3.8710
.4044
.75542
1.000
-2.2044
2.0710
.867
-1.4044
2.8710
90
.7333
.75542
Based on observed means.
The error term is Mean Square(Error) = 4.280.
*. The mean difference is significant at the .05 level.
-2.1956
Homogeneous Subsets
Hasil
Tukey HSD
Lama
Reaksi
(Menit)
Subset
N
1
2
3
90
15
15.9333
120
15
16.6667
16.6667
60
15
16.7333
16.7333
30
15
18.4000
0
15
21.0000
Sig.
.826
.164
1.000
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 4.280.
civ
Universitas Sumatera Utara
Lampiran 19. Analisis Statistik LDL Pemberian Minyak Kelapa Sawit
Univariate Analysis of Variance
Descriptive Statistics
Dependent Variable:Hasil
Lama
Reaksi Hari_K
(Menit) e
Mean
0
0
18.1333
3.70045
3
1
21.0667
2.89367
3
7
21.0667
3.07463
3
14
11.4667
3.13900
3
21
5.6000
2.69072
3
Total
15.4667
6.79692
15
0
20.2000
4.13280
3
1
26.6667
4.31432
3
7
30.2667
2.99555
3
14
15.0000
3.81576
3
21
12.6000
2.60000
3
Total
20.9467
7.59453
15
0
20.0667
4.60579
3
1
45.0667
3.40196
3
7
27.8000
1.38564
3
14
19.5333
1.66533
3
21
11.5333
.70238
3
Total
24.8000
11.99286
15
0
15.4667
2.91433
3
1
45.8667
4.34665
3
7
31.9333
2.30072
3
14
19.5333
2.11975
3
21
13.0667
1.66533
3
Total
25.1733
12.87142
15
0
17.8667
3.06159
3
1
43.4667
3.66788
3
7
35.8000
3.53836
3
14
19.0000
3.90384
3
21
11.6667
3.80175
3
Total
25.5600
12.79603
15
0
18.3467
3.63119
15
1
36.4267
11.26102
15
7
29.3733
5.58512
15
14
16.9067
4.20942
15
21
10.8933
3.51963
15
Total
22.3893
11.14033
75
30
60
90
120
Total
Std. Deviation
N
cv
Universitas Sumatera Utara
Lanjutan Lampiran 19.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Type III Sum
Source
of Squares
Partial Eta
df
Mean Square
F
Sig.
Squared
Corrected Model
8668.791a
24
361.200
35.060
.000
.944
Intercept
37596.169
1
37596.169
3.649E3
.000
.986
Menit_Ke
1104.295
4
276.074
26.797
.000
.682
Hari_Ke
6365.757
4
1591.439
154.473
.000
.925
1198.739
16
74.921
7.272
.000
.699
Error
515.120
50
10.302
Total
46780.080
75
9183.911
74
Menit_Ke *
Hari_Ke
Corrected Total
a. R Squared = .944 (Adjusted R Squared = .917)
Post Hoc Tests
Lama Reaksi (Menit)
Multiple Comparisons
Hasil
Tukey HSD
(I)
Lama
Reaksi
(Menit)
0
(J)
Lama
Reaksi Mean Difference
(Menit)
(I-J)
Std. Error
Lower Bound
Upper Bound
1.17203
.000
-8.7966
-2.1634
*
1.17203
.000
-12.6500
-6.0167
*
1.17203
.000
-13.0233
-6.3900
*
1.17203
.000
-13.4100
-6.7767
*
1.17203
.000
2.1634
8.7966
*
1.17203
.015
-7.1700
-.5367
90
*
-4.2267
1.17203
.006
-7.5433
-.9100
120
-4.6133*
1.17203
.002
-7.9300
-1.2967
9.3333*
1.17203
.000
6.0167
12.6500
*
1.17203
.015
.5367
7.1700
90
-.3733
1.17203
.998
-3.6900
2.9433
120
-.7600
1.17203
.966
-4.0766
2.5566
30
90
120
0
60
60
Sig.
*
60
30
95% Confidence Interval
0
30
-5.4800
-9.3333
-9.7067
-10.0933
5.4800
-3.8533
3.8533
cvi
Universitas Sumatera Utara
Lanjutan Lampiran 19.
90
9.7067*
1.17203
.000
*
1.17203
60
.3733
1.17203
120
-.3867
*
*
.7600
0
30
120
4.2267
0
10.0933
30
4.6133
60
6.3900
13.0233
.006
.9100
7.5433
.998
-2.9433
3.6900
1.17203
.997
-3.7033
2.9300
1.17203
.000
6.7767
13.4100
1.17203
.002
1.2967
7.9300
1.17203
.966
-2.5566
4.0766
.997
-2.9300
3.7033
90
.3867
1.17203
Based on observed means.
The error term is Mean Square(Error) = 10.302.
*. The mean difference is significant at the .05 level.
Homogeneous Subsets
Hasil
Tukey HSD
Lama
Reaksi
(Menit)
Subset
N
1
2
3
0
15
15.4667
30
15
20.9467
60
15
24.8000
90
15
25.1733
120
15
25.5600
Sig.
1.000
1.000
.966
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 10.302.
cvii
Universitas Sumatera Utara
Lampiran 20. Pengujian Beda Nilai Rata-Rata Kadar Kolesterol Total Setelah
Pemberian Lemak Sapi dan Minyak Kelapa Sawit Hasil
Interesterifikasi
Rata-rata Kadar (n=3) Kolesterol total (mg/dl)
Hari keLemak Sapi
Minyak Kelapa Sawit
0
44.67
44.00
1
83.67
74.33
7
94.67
53.67
14
61.33
45.33
21
58.67
43.00
Total
343.01
260.33
Mean
X a = 68.60
X b = 52.07
St. Deviasi
STD a = 20.19
STD b = 13.14
Kedua sampel adalah independen, maka dilakukan uji t beda antara dua mean dengan
taraf kepercayaan 95% untuk mengetahui apakah rata-rata kedua sampel sama (σ 1 =
σ 2 ) atau berbeda (σ 1 ≠ σ 2 ) .
1. H o : σ 1 = σ 2
H1 : σ 1 ≠ σ 2
2. Nilai kritis t yang diperoleh dari tabel (t 0,05/2 (8))adalah = 2,3060
Daerah kritis penerimaan : -2,3060 ≤ t o ≤ 2,3060
Daerah kritis penolakan
: t o < -2,3060 dan t o > 2,3060
3. T hitung antara dua mean
cviii
Universitas Sumatera Utara
Lanjutan Lampiran 20.
4. Karena t o = 2.81 > 2.3060, maka hipotesa H o ditolak. Berarti terdapat
perbedaan signifikan rata-rata kadar kolesterol total lemak sapi dan minyak
kelapa sawit hasil interesterifikasi.
cix
Universitas Sumatera Utara
Lampiran 21. Pengujian Beda Nilai Rata-Rata Kadar Trigliserida Setelah Pemberian
Lemak Sapi dan Minyak Kelapa Sawit Hasil Interesterifikasi
Rata-rata Kadar (n=3) Trigliserida (mg/dl)
Hari keLemak Sapi
Minyak Kelapa Sawit
0
48.67
48.00
1
85.67
76.33
7
98.67
57.67
14
58.33
42.33
21
54.67
39.00
Total
346.01
263.33
Mean
X a = 69.20
X b = 52.67
St. Deviasi
STD a = 21.74
STD b = 15.00
Kedua sampel adalah independen, maka dilakukan uji t beda antara dua mean dengan
taraf kepercayaan 95% untuk mengetahui apakah rata-rata kedua sampel sama (σ 1 =
σ 2 ) atau berbeda (σ 1 ≠ σ 2 ) .
1. H o : σ 1 = σ 2
H1 : σ 1 ≠ σ 2
2. Nilai kritis t yang diperoleh dari tabel (t 0,05/2 (8))adalah = 2,3060
Daerah kritis penerimaan : -2,3060 ≤ t o ≤ 2,3060
Daerah kritis penolakan
: t o < -2,3060 dan t o > 2,3060
3. T hitung antara dua mean
cx
Universitas Sumatera Utara
Lanjutan Lampiran 21.
4. Karena t o = 2.55 > 2.3060, maka hipotesa H o ditolak. Berarti terdapat
perbedaan signifikan rata-rata kadar trigliserida lemak sapi dan minyak kelapa
sawit hasil interesterifikasi.
cxi
Universitas Sumatera Utara
Lampiran 22. Pengujian Beda Nilai Rata-Rata Kadar HDL Setelah Pemberian
Lemak Sapi dan Minyak Kelapa Sawit Hasil Interesterifikasi
Rata-rata Kadar (n=3) HDL (mg/dl)
Hari keLemak Sapi
Minyak Kelapa Sawit
0
14.67
14.33
1
14.00
14.00
7
14.33
14.33
14
14.00
17.33
21
14.67
23.67
Total
71.67
83.66
Mean
X a = 14.33
X b = 16.73
St. Deviasi
STD a = 0.34
STD b = 4.11
Kedua sampel adalah independen, maka dilakukan uji t beda antara dua mean dengan
taraf kepercayaan 95% untuk mengetahui apakah rata-rata kedua sampel sama (σ 1 =
σ 2 ) atau berbeda (σ 1 ≠ σ 2 ) .
1. H o : σ 1 = σ 2
H1 : σ 1 ≠ σ 2
2. Nilai kritis t yang diperoleh dari tabel (t 0,05/2 (8))adalah = 2,3060
Daerah kritis penerimaan : -2,3060 ≤ t o ≤ 2,3060
Daerah kritis penolakan
: t o < -2,3060 dan t o > 2,3060
3. T hitung antara dua mean
cxii
Universitas Sumatera Utara
Lanjutan Lampiran 22.
4. Karena t o = 3.05 > 2.3060, maka hipotesa H o ditolak. Berarti terdapat
perbedaan signifikan rata-rata kadar HDL lemak sapi dan minyak kelapa sawit
hasil interesterifikasi.
cxiii
Universitas Sumatera Utara
Lampiran 23. Pengujian Beda Nilai Rata-Rata Kadar LDL Setelah Pemberian
Lemak Sapi dan Minyak Kelapa Sawit Hasil Interesterifikasi
Rata-rata Kadar (n=3) LDL (mg/dl)
Hari keLemak Sapi
Minyak Kelapa Sawit
0
20.27
20.07
1
52.53
45.07
7
60.60
27.80
14
35.67
19.53
21
33.06
11.53
Total
202.13
124.00
Mean
X a = 40.43
X b = 24.80
St. Deviasi
STDa =16.10
STD b = 12.71
Kedua sampel adalah independen, maka dilakukan uji t beda antara dua mean dengan
taraf kepercayaan 95% untuk mengetahui apakah rata-rata kedua sampel sama (σ 1 =
σ 2 ) atau berbeda (σ 1 ≠ σ 2 ) .
1. H o : σ 1 = σ 2
H1 : σ 1 ≠ σ 2
2. Nilai kritis t yang diperoleh dari tabel (t 0,05/2 (8))adalah = 2,3060
Daerah kritis penerimaan : -2,3060 ≤ t o ≤ 2,3060
Daerah kritis penolakan
: t o < -2,3060 dan t o > 2,3060
3. T hitung antara dua mean
cxiv
Universitas Sumatera Utara
Lanjutan Lampiran 23.
4. Karena t o = 3.07 > 2.3060, maka hipotesa H o ditolak. Berarti terdapat
perbedaan signifikan rata-rata kadar LDL lemak sapi dan minyak kelapa sawit
hasil interesterifikasi.
cxv
Universitas Sumatera Utara
Lampiran 24. Tabel Nilai Kritik Distribusi t
cxvi
Universitas Sumatera Utara
Lampiran 25. Gambar Alat
Microlab 300 (Vital Scientific)
Melting Point Aparatus (Stuart)
cxvii
Universitas Sumatera Utara
Lampiran 26. Surat Keterangan Pemakaian Laboratorium Kesehatan Dinas
Kesehatan Propinsi Sumatera Utara
cxviii
Universitas Sumatera Utara
Lampiran 27. Surat Keterangan Pemakaian Laboratorium Penelitian Fakultas
Farmasi USU
cxix
Universitas Sumatera Utara
Lampiran 28. Etichal Clearance Komite Etik Penelitian Hewan
cxx
Universitas Sumatera Utara
150 ml sampel
Ditambah 15 ml NaOCH3 0,1N
Diaduk dengan kecepatan 4000 rpm
pada suhu 60-70oC selama variasi
waktu (30,60,90,120 menit)
Hasil reaksi
Ditambah asam Sitrat 20% (b/v)
Dimasukkan dalam corong pisah
Ditambah 150 ml n-heksan
Dicuci dengan akuades 3 (tiga) kali
Lapisan atas
Lapisan bawah
Ditambah Na2SO4 anhidrat
Disaring
dirotarievaporasi
Ditentukan titik leburnya
Larutan siap diuji
pada marmut
lxxiii
Universitas Sumatera Utara
Lampiran 2. Bagan Alur Penentuan Titik Lebur
Sampel cair
Dimasukkan dalam pipa kapiler Ø 1 mm
Dibekukan selama 24 jam dalam
freezer
Dimasukkan dalam alat Melting
Point Aparatus
Dinaikkan suhu pada alat secara
bertahap & perlahan hingga meleleh
Dicatat titik leburnya
Titik lebur sampel
lxxiv
Universitas Sumatera Utara
Lampiran 3. Bagan Alur Pengujian Efek terhadap Profil Lipida Serum Darah
Marmut
Marmut
Dikondisikan selama 2 minggu
Diukur serum
d h
Diberi perlakuan selama
21 hari
Kadar Profil
Lipida Awal
Diukur kadar profil
lipida pada hari ke-1,7
dan 14
Kadar Profil
Lipida
lxxv
Universitas Sumatera Utara
Lampiran 4. Bagan Alur Pengambilan Serum Darah Marmut
Marmut
Dipuasakan selama 10-14 jam
Dipangkas bulu kakinya
Disikat kuku kakinya dengan
sikat gigi basah
Kuku yang bersih
Dibilas dengan alkohol
Dipotong dengan pemotong
kuku sampai berdarah
Darah marmut
Didiamkan selama 10 menit
Disentrifugasi dengan kecepatan
3000 rpm selama 15 menit
Diambil cairan bening
Serum siap diukur
lxxvi
Universitas Sumatera Utara
Lampiran 5. Bagan Alur Pengukuran Kadar Kolesterol
Serum
Dipipet sebanyak 10 µl
Dimasukkan dalam tabung reaksi
yang berisi larutan reagensia
kolesterol sebanyak 1 ml
Dihomogenkan
Diinkubasi pada suhu kamar
selama 20 menit
Diukur pada mikrolab 300 dengan
panjang gelombang 546 nm
Dicatat hasilnya
Kadar kolesterol total
lxxvii
Universitas Sumatera Utara
Lampiran 6. Bagan Alur Pengukuran Kadar Trigliserida
Serum
Dipipet sebanyak 10 µl
Dimasukkan dalam tabung reaksi
yang berisi larutan reagensia
trigliserida sebanyak 1 ml
Dihomogenkan
Diinkubasi pada suhu kamar
selama 20 menit
Diukur pada mikrolab 300 dengan
panjang gelombang 546 nm
Dicatat hasilnya
Kadar trigliserida
lxxviii
Universitas Sumatera Utara
Lampiran 7. Bagan Alur Pengukuran Kadar HDL
Serum
Dipipet sebanyak 100 µl
Dimasukkan dalam tabung reaksi
yang berisi larutan reagensia
kolesterol HDL sebanyak 1 ml
Dihomogenkan
Diinkubasi pada suhu kamar
selama 20 menit
Diukur pada mikrolab 300 dengan
panjang gelombang 546 nm
Dicatat hasilnya
Kadar HDL
lxxix
Universitas Sumatera Utara
Lampiran 8. Data Kadar Kolesterol Total Serum Darah Marmut (mg/dl)
Perlakuan
A
B
C
D
E
F
G
H
I
J
Kadar Kolesterol (mg/dl)
Berat
Badan
(gram)
Awal
1 hari
7 hari
14 hari
21 hari
256
258
240
236
222
208
204
226
213
254
249
238
227
244
208
266
218
208
257
268
261
265
299
275
211
214
207
241
232
217
34
36
39
48
38
45
41
47
46
43
40
46
48
45
42
49
45
42
47
46
42
45
41
46
34
40
42
45
39
41
51
55
53
62
65
60
81
88
82
82
84
88
84
87
85
51
47
45
55
54
50
76
72
75
72
79
75
70
72
75
71
73
75
77
73
76
96
93
95
99
92
95
97
93
91
53
48
46
58
59
57
51
56
54
56
60
58
61
64
66
76
70
74
74
69
73
63
60
61
64
60
61
66
63
59
48
43
41
46
48
40
42
48
46
44
42
45
43
49
42
78
72
77
68
65
66
60
58
58
61
57
58
63
59
54
46
42
40
43
44
51
40
46
43
42
41
43
40
47
40
lxxx
Universitas Sumatera Utara
Lampiran 9. Data Kadar Trigliserida Serum Darah Marmut (mg/dl)
Berat
Perlakuan Badan
(gram)
A
256
B
C
D
E
F
G
H
I
J
258
240
236
222
208
204
226
213
254
249
238
227
244
208
266
218
208
257
268
261
265
299
275
211
214
207
241
232
217
Kadar Trigliserida (mg/dl)
Awal
1 hari
7 hari
14 hari
21 hari
38
40
43
52
42
49
45
51
50
47
44
50
52
49
46
53
49
46
51
50
46
49
45
50
38
44
46
49
43
45
53
57
55
64
67
62
83
90
84
84
86
90
86
89
87
53
49
47
57
56
52
78
74
77
74
81
77
72
74
77
75
77
79
81
77
80
100
97
99
103
96
99
101
97
95
57
52
50
62
63
61
55
60
58
60
64
62
65
68
70
73
67
71
71
66
70
60
57
58
61
57
58
63
60
56
45
40
38
43
45
37
39
45
43
41
39
42
40
46
39
74
68
73
64
61
62
56
54
54
57
53
54
59
55
50
42
38
36
39
40
47
36
42
39
38
37
39
36
43
36
lxxxi
Universitas Sumatera Utara
Lampiran 10. Data Kadar HDL Serum Darah Marmut (mg/dl)
Perlakuan
A
B
C
D
E
F
G
H
I
J
Kadar HDL (mg/dl)
Berat
Badan
(gram)
Awal
1 hari
7 hari
14 hari
21 hari
256
258
240
236
222
208
226
204
213
254
249
238
227
244
208
266
218
208
257
268
261
265
299
275
211
214
207
241
232
217
11
10
15
16
17
14
11
19
14
15
13
18
13
12
14
16
19
17
12
17
16
14
17
12
14
13
17
15
16
13
11
9
15
16
18
14
11
18
13
15
12
18
14
12
13
16
18
16
12
18
16
14
16
12
13
12
17
15
15
12
12
10
15
17
18
15
11
18
14
16
11
18
14
12
14
17
18
17
12
17
17
13
17
13
12
13
16
16
14
13
11
10
14
16
17
13
12
17
13
16
13
19
15
13
15
24
26
23
20
22
22
16
20
16
14
15
19
20
17
15
12
9
13
15
16
13
13
19
12
15
12
18
15
14
14
29
31
28
24
25
26
22
26
23
20
20
24
25
23
21
lxxxii
Universitas Sumatera Utara
Lampiran 11. Data Kadar LDL Serum Darah Marmut
Perlakuan
A
B
C
D
E
F
G
H
I
J
Kadar LDL (mg/dl)
Berat
Badan
(gram)
Awal
1 hari
7 hari
14 hari
21 hari
256
250
240
236
222
208
226
204
213
254
249
238
227
244
208
266
218
208
257
268
261
265
299
275
211
214
207
241
232
217
15.4
18
15.4
21.6
12.6
21.2
21
17.8
22
18.6
18.2
18
24.6
23.2
18.8
22.4
16.2
15.8
24.8
19
16.8
21.2
15
24
12.4
18.2
15.8
20.2
14.4
19
29.4
34.6
27
33.2
33.6
33.6
53.4
52
52.2
50.2
54.8
52
52.8
57.2
54.6
24.4
19.2
19.6
31.6
24.8
23.6
46.4
41.2
47.6
44.2
50.8
42.6
40.6
42.2
47.6
44
47.6
44.2
43.8
39.6
45
65
55.6
61.2
62.4
61.8
57.2
62.8
61.6
58
24.6
19.6
19
33.6
29.4
27.8
27
27
29.4
32
34.2
29.6
32
36.4
39
50.4
46.6
45.8
43.8
38.8
46
39
31.6
36.4
35.8
35.6
30.4
38.4
38
32.8
15
9
10.4
17.4
17
10.6
18.2
19
21.4
21.8
19.2
17.6
15
22.8
19.2
51.2
49.4
49.4
40.2
36.8
40.6
35.8
28.2
35.2
34.6
34.4
29.2
36.2
34
30
8.6
3.4
4.8
11.2
11
15.6
10.8
11.6
12.2
14.4
13.6
11.2
7.8
15.4
11.8
lxxxiii
Universitas Sumatera Utara
Lampiran 12. Analisis Statistik Kolesterol Total Pemberian Lemak Sapi
Univariate Analysis of Variance
Dependent Variable:Hasil Pengamatan
Lama
Lama
Reaksi Percoba
(menit) an
0
30
60
90
120
Total
Mean
Std. Deviation
N
0
36.3333
2.51661
3
1
53.0000
2.00000
3
7
73.0000
2.00000
3
14
73.3333
3.05505
3
21
75.6667
3.21455
3
Total
62.2667
16.02439
15
0
43.6667
5.13160
3
1
62.3333
2.51661
3
7
75.3333
2.08167
3
14
72.0000
2.64575
3
21
66.3333
1.52753
3
Total
63.9333
11.75625
15
0
44.6667
3.21455
3
1
83.6667
3.78594
3
7
94.6667
1.52753
3
14
61.3333
1.52753
3
21
58.6667
1.15470
3
Total
68.6000
18.81033
15
0
43.0000
3.00000
3
1
84.6667
3.05505
3
7
95.3333
3.51188
3
14
61.6667
2.08167
3
21
58.6667
2.08167
3
Total
68.6667
19.64567
15
0
45.0000
3.00000
3
1
85.3333
1.52753
3
7
93.6667
3.05505
3
14
62.6667
3.51188
3
21
58.6667
4.50925
3
Total
69.0667
18.71007
15
0
42.5333
4.42181
15
1
73.8000
14.16838
15
7
86.4000
10.60189
15
14
66.2000
5.94258
15
21
63.6000
7.34652
15
Total
66.5067
17.00189
75
lxxxiv
Universitas Sumatera Utara
Lanjutan Lampiran 12.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Pengamatan
Type III
Sum of
Squares
Source
Corrected
Model
Intercept
20983.413a
Menit_Ke
603.013
Hari_Ke
331735.25
3
15483.013
Menit_Ke *
Hari_Ke
Error
Total
Mean
Square
df
F
Sig.
107.32
1
331735.25 4.072E
1
3
4
24
874.309
4
16
407.333
353126.00
0
50
Observed
Powerb
.000
.981
2575.705
1.000
.000
.999 40720.367
1.000
150.753 18.505
.000
.597
74.020
1.000
475.13
3
.000
.974
1900.534
1.000
306.087 37.572
.000
.923
601.152
1.000
4 3870.753
4897.387
Partial Eta Noncent.
Squared Parameter
8.147
75
Corrected
21390.747
74
Total
a. R Squared = .981 (Adjusted R Squared =
.972)
Post Hoc Tests
Lama Reaksi (menit)
Multiple Comparisons
Hasil Pengamatan
Tukey HSD
(I)
Lama
Reaksi
(menit)
(J)
Lama
Reaksi
(menit)
0
30
-1.6667
1.04222
.505
-4.6159
1.2826
60
*
1.04222
.000
-9.2826
-3.3841
*
1.04222
.000
-9.3493
-3.4507
*
-6.8000
1.04222
.000
-9.7493
-3.8507
1.6667
1.04222
.505
-1.2826
4.6159
*
1.04222
.000
-7.6159
-1.7174
*
1.04222
.000
-7.6826
-1.7841
*
1.04222
.000
-8.0826
-2.1841
*
1.04222
.000
3.3841
9.2826
*
1.04222
.000
1.7174
7.6159
90
-.0667
1.04222
1.000
-3.0159
2.8826
120
-.4667
1.04222
.991
-3.4159
2.4826
90
120
30
0
60
90
120
60
0
30
95% Confidence Interval
Mean Difference
(I-J)
-6.3333
-6.4000
-4.6667
-4.7333
-5.1333
6.3333
4.6667
Std. Error
Sig.
Lower Bound
Upper Bound
lxxxv
Universitas Sumatera Utara
Lanjutan Lampiran 12
90
6.4000*
1.04222
*
1.04222
60
.0667
1.04222
120
-.4000
1.04222
*
*
.4667
0
30
120
4.7333
0
6.8000
30
5.1333
60
3.4507
9.3493
.000
1.7841
7.6826
1.000
-2.8826
3.0159
.995
-3.3493
2.5493
1.04222
.000
3.8507
9.7493
1.04222
.000
2.1841
8.0826
1.04222
.991
-2.4826
3.4159
.995
-2.5493
3.3493
90
.4000
1.04222
Based on observed means.
The error term is Mean Square(Error) = 8.147.
*. The mean difference is significant at the .05 level.
.000
Homogeneous Subsets
Hasil Pengamatan
Tukey HSD
Lama
Reaksi
(menit)
Subset
N
1
2
0
15
62.2667
30
15
63.9333
60
15
68.6000
90
15
68.6667
120
15
69.0667
Sig.
.505
.991
Means for groups in homogeneous subsets are
displayed.
Based on observed means.
The error term is Mean Square(Error) = 8.147.
lxxxvi
Universitas Sumatera Utara
Lampiran 13. Analisis Statistik Trigliserida Pemberian Lemak Sapi
Univariate Analysis of Variance
Dependent Variable:Hasil Pengamatan
Lama
Reaksi
(menit) Lama Percobaan
0
30
60
90
120
Total
Mean
Std. Deviation
N
0
40.3333
2.51661
3
1
55.0000
2.00000
3
7
77.0000
2.00000
3
14
70.3333
3.05505
3
21
71.6667
3.21455
3
Total
62.8667
14.08072
15
0
47.6667
5.13160
3
1
64.3333
2.51661
3
7
79.3333
2.08167
3
14
69.0000
2.64575
3
21
62.3333
1.52753
3
Total
64.5333
10.94706
15
0
48.6667
3.21455
3
1
85.6667
3.78594
3
7
98.6667
1.52753
3
14
58.3333
1.52753
3
21
54.6667
1.15470
3
Total
69.2000
20.23505
15
0
47.0000
3.00000
3
1
86.6667
3.05505
3
7
99.3333
3.51188
3
14
58.6667
2.08167
3
21
54.6667
2.08167
3
Total
69.2667
20.98117
15
0
49.0000
3.00000
3
1
87.3333
1.52753
3
7
97.6667
3.05505
3
14
59.6667
3.51188
3
21
54.6667
4.50925
3
Total
69.6667
20.09146
15
0
46.5333
4.42181
15
1
75.8000
14.16838
15
7
90.4000
10.60189
15
14
63.2000
5.94258
15
21
59.6000
7.34652
15
Total
67.1067
17.47707
75
lxxxvii
Universitas Sumatera Utara
Lanjutan Lampiran 13.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Pengamatan
Type III Sum
of Squares df
Source
Corrected
Model
Intercept
Menit_Ke
Hari_Ke
Menit_Ke *
Hari_Ke
Error
Total
Corrected Total
22195.813a
337747.853
Mean
Square
24
F
Sig.
924.826 113.522 .000
1 337747.853
4.146E
.000
4
Partial Eta Noncent.
Squared Parameter
.982
Observed
Powerb
2724.527
1.000
.999 41458.411
1.000
603.013
4
150.753 18.505 .000
.597
74.020
1.000
16695.413
4
4173.853 512.339 .000
.976
2049.355
1.000
4897.387
16
306.087 37.572 .000
.923
601.152
1.000
407.333
50
360351.000
75
22603.147
74
8.147
Post Hoc Tests
Lama Reaksi (menit)
Multiple Comparisons
Hasil Pengamatan
Tukey HSD
(I)
Lama
Reaksi
(menit)
(J)
Lama
Reaksi
(menit)
0
30
-1.6667
1.04222
.505
-4.6159
1.2826
60
*
1.04222
.000
-9.2826
-3.3841
*
1.04222
.000
-9.3493
-3.4507
*
-6.8000
1.04222
.000
-9.7493
-3.8507
1.6667
1.04222
.505
-1.2826
4.6159
*
1.04222
.000
-7.6159
-1.7174
*
1.04222
.000
-7.6826
-1.7841
*
1.04222
.000
-8.0826
-2.1841
0
6.3333
*
1.04222
.000
3.3841
9.2826
30
4.6667*
1.04222
.000
1.7174
7.6159
90
-.0667
1.04222
1.000
-3.0159
2.8826
120
-.4667
1.04222
.991
-3.4159
2.4826
90
120
30
0
60
90
120
60
95% Confidence Interval
Mean Difference
(I-J)
-6.3333
-6.4000
-4.6667
-4.7333
-5.1333
Std. Error
Sig.
Lower Bound
Upper Bound
lxxxviii
Universitas Sumatera Utara
Lanjutan Lampiran 13.
90
120
0
6.4000*
1.04222
.000
3.4507
9.3493
30
4.7333*
1.04222
.000
1.7841
7.6826
60
.0667
1.04222
1.000
-2.8826
3.0159
120
-.4000
1.04222
.995
-3.3493
2.5493
6.8000
*
1.04222
.000
3.8507
9.7493
5.1333
*
1.04222
.000
2.1841
8.0826
.4667
1.04222
.991
-2.4826
3.4159
.995
-2.5493
3.3493
0
30
60
90
.4000
1.04222
Based on observed means.
The error term is Mean Square(Error) = 8.147.
*. The mean difference is significant at the .05 level.
Homogeneous Subsets
Hasil Pengamatan
Tukey HSD
Lama
Reaksi
(menit)
0
30
60
90
120
Sig.
Subset
N
1
15
15
15
15
15
2
62.8667
64.5333
.505
69.2000
69.2667
69.6667
.991
Means for groups in homogeneous subsets are
displayed.
Based on observed means.
The error term is Mean Square(Error) = 8.147.
lxxxix
Universitas Sumatera Utara
Lampiran 14. Analisis Statistik HDL Pemberian Lemak Sapi
Univariate Analysis of Variance
Descriptive Statistics
Dependent Variable:Hasil
Lama
Reaksi Hari_K
(Menit) e
Mean
0
0
12.0000
2.64575
3
1
11.6667
3.05505
3
7
12.3333
2.51661
3
14
11.6667
2.08167
3
21
11.3333
2.08167
3
Total
11.8000
2.14476
15
0
15.6667
1.52753
3
1
16.0000
2.00000
3
7
16.6667
1.52753
3
14
15.3333
2.08167
3
21
14.6667
1.52753
3
Total
15.6667
1.63299
15
0
14.6667
4.04145
3
1
14.0000
3.60555
3
7
14.3333
3.51188
3
14
14.0000
2.64575
3
21
14.6667
3.78594
3
Total
14.3333
3.01583
15
0
15.3333
2.51661
3
1
15.0000
3.00000
3
7
15.0000
3.60555
3
14
16.0000
3.00000
3
21
15.0000
3.00000
3
Total
30
60
90
120
Total
Std. Deviation
N
15.2667
2.60403
15
0
13.0000
1.00000
3
1
13.0000
1.00000
3
7
13.3333
1.15470
3
14
14.3333
1.15470
3
21
14.3333
.57735
3
Total
13.6000
1.05560
15
0
14.1333
2.61498
15
1
13.9333
2.76371
15
7
14.3333
2.71679
15
14
14.2667
2.46306
15
21
14.0000
2.50713
15
Total
14.1333
2.54863
75
xc
Universitas Sumatera Utara
Lanjutan Lampiran 14.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Type III Sum
Source
of Squares
Partial Eta
df
Mean Square
F
Sig.
Squared
158.667a
24
6.611
1.027
.454
.330
14981.333
1
14981.333
2.326E3
.000
.979
141.067
4
35.267
5.476
.001
.305
1.733
4
.433
.067
.991
.005
15.867
16
.992
.154
1.000
.047
Error
322.000
50
6.440
Total
15462.000
75
480.667
74
Corrected Model
Intercept
Menit_Ke
Hari_Ke
Menit_Ke *
Hari_Ke
Corrected Total
a. R Squared = .330 (Adjusted R Squared = .009)
Post Hoc Tests
Lama Reaksi (Menit)
Multiple Comparisons
Hasil
Tukey HSD
(I)
Lama
Reaksi
(Menit)
0
(J)
Lama
Reaksi Mean Difference
(Menit)
(I-J)
Std. Error
60
Sig.
Lower Bound
Upper Bound
*
.92664
.001
-6.4889
-1.2444
60
-2.5333
.92664
.063
-5.1556
.0889
90
*
.92664
.004
-6.0889
-.8444
-1.8000
.92664
.309
-4.4222
.8222
*
.92664
.001
1.2444
6.4889
60
1.3333
.92664
.606
-1.2889
3.9556
90
.4000
.92664
.993
-2.2222
3.0222
120
2.0667
.92664
.186
-.5556
4.6889
0
2.5333
.92664
.063
-.0889
5.1556
30
-1.3333
.92664
.606
-3.9556
1.2889
90
-.9333
.92664
.851
-3.5556
1.6889
120
.7333
.92664
.932
-1.8889
3.3556
30
120
30
95% Confidence Interval
0
-3.8667
-3.4667
3.8667
xci
Universitas Sumatera Utara
Lanjutan Lampiran 14.
90
120
0
3.4667*
.92664
.004
.8444
6.0889
30
-.4000
.92664
.993
-3.0222
2.2222
60
.9333
.92664
.851
-1.6889
3.5556
120
1.6667
.92664
.386
-.9556
4.2889
0
1.8000
.92664
.309
-.8222
4.4222
30
-2.0667
.92664
.186
-4.6889
.5556
60
-.7333
.92664
.932
-3.3556
1.8889
.386
-4.2889
.9556
90
-1.6667
.92664
Based on observed means.
The error term is Mean Square(Error) = 6.440.
*. The mean difference is significant at the .05 level.
Homogeneous Subsets
Hasil
Tukey HSD
Lama
Reaksi
(Menit)
Subset
N
1
2
0
15
11.8000
120
15
13.6000
13.6000
60
15
14.3333
14.3333
90
15
15.2667
30
15
15.6667
Sig.
.063
.186
Means for groups in homogeneous subsets
are displayed.
Based on observed means.
The error term is Mean Square(Error) =
6.440.
xcii
Universitas Sumatera Utara
Lampiran 15. Analisis Statistik LDL Pemberian Lemak Sapi
Univariate Analysis of Variance
Descriptive Statistics
Dependent Variable:Hasil
Lama
Reaksi Hari_K
(Menit) e
Mean
0
0
16.2667
1.50111
3
1
30.3333
3.88501
3
7
45.2667
2.02320
3
14
47.6000
2.45764
3
21
50.0000
1.03923
3
Total
37.8933
13.40892
15
0
18.4667
5.08462
3
1
33.4667
.23094
3
7
42.8000
2.83549
3
14
42.8667
3.68963
3
21
39.2000
2.08806
3
Total
35.3600
9.82015
15
0
20.2667
2.19393
3
1
52.5333
.75719
3
7
60.6000
4.72864
3
14
35.6667
3.75411
3
21
33.0667
4.22532
3
Total
40.4267
15.18869
15
0
18.2667
.30551
3
1
52.3333
2.31805
3
7
60.4667
2.84488
3
14
33.9333
3.06159
3
21
32.7333
3.06159
3
Total
39.5467
15.72159
15
0
22.2000
3.02655
3
1
54.8667
2.21209
3
7
60.8000
2.49800
3
14
36.4000
3.12410
3
21
33.4000
3.14325
3
Total
41.5333
14.94192
15
0
19.0933
3.21414
15
1
44.7067
11.07993
15
7
53.9867
8.85453
15
14
39.2933
5.98707
15
21
37.6800
7.26825
15
Total
38.9520
13.76996
75
30
60
90
120
Total
Std. Deviation
N
xciii
Universitas Sumatera Utara
Lanjutan Lampiran 15.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Type III Sum
of Squares
Source
df
Mean Square
F
Sig.
Partial Eta
Squared
Corrected Model
13606.441a
24
566.935
66.725
.000
.970
Intercept
113794.373
1
113794.373
1.339E4
.000
.996
348.222
4
87.055
10.246
.000
.450
9828.878
4
2457.219
289.203
.000
.959
3429.341
16
214.334
25.226
.000
.890
424.827
50
8.497
127825.640
75
14031.267
74
Menit_Ke
Hari_Ke
Menit_Ke *
Hari_Ke
Error
Total
Corrected Total
a. R Squared = .970 (Adjusted R Squared = .955)
Post Hoc Tests
Lama Reaksi (Menit)
Multiple Comparisons
Hasil
Tukey HSD
(I)
Lama
Reaksi
(Menit)
(J)
Lama
Reaksi
(Menit)
0
30
2.5333
1.06436
.138
-.4786
5.5453
60
-2.5333
1.06436
.138
-5.5453
.4786
90
-1.6533
1.06436
.534
-4.6653
1.3586
120
*
-3.6400
1.06436
.011
-6.6519
-.6281
0
-2.5333
1.06436
.138
-5.5453
.4786
*
1.06436
.000
-8.0786
-2.0547
*
1.06436
.002
-7.1986
-1.1747
*
-6.1733
1.06436
.000
-9.1853
-3.1614
2.5333
1.06436
.138
-.4786
5.5453
*
5.0667
1.06436
.000
2.0547
8.0786
30
-5.0667
90
-4.1867
0
30
90
Std. Error
Sig.
Lower Bound
Upper Bound
.8800
1.06436
.921
-2.1319
3.8919
-1.1067
1.06436
.836
-4.1186
1.9053
0
1.6533
1.06436
.534
-1.3586
4.6653
30
4.1867
*
1.06436
.002
1.1747
7.1986
60
-.8800
1.06436
.921
-3.8919
2.1319
120
-1.9867
1.06436
.348
-4.9986
1.0253
120
90
Mean Difference
(I-J)
60
120
60
95% Confidence Interval
xciv
Universitas Sumatera Utara
Lanjutan Lampiran 15.
120
*
1.06436
.011
.6281
6.6519
30
*
6.1733
1.06436
.000
3.1614
9.1853
60
1.1067
1.06436
.836
-1.9053
4.1186
90
1.9867
1.06436
.348
-1.0253
4.9986
0
3.6400
Based on observed means.
The error term is Mean Square(Error) = 8.497.
*. The mean difference is significant at the .05 level.
Homogeneous Subsets
Hasil
Tukey HSD
Lama
Reaksi
(Menit)
Subset
N
1
2
3
30
15
35.3600
0
15
37.8933
37.8933
90
15
39.5467
39.5467
60
15
40.4267
40.4267
120
15
41.5333
Sig.
.138
.138
.348
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 8.497.
xcv
Universitas Sumatera Utara
Lampiran 16. Analisis Statistik Kolesterol Pemberian Minyak Kelapa Sawit
Univariate Analysis of Variance
Descriptive Statistics
Dependent Variable:Hasil Pengamatan
Lama
Lama
Reaksi Percoba
(menit) an
0
30
60
90
120
Total
Mean
Std. Deviation
N
0
45.3333
3.51188
3
1
47.6667
3.05505
3
7
49.0000
3.60555
3
14
44.0000
3.60555
3
21
42.6667
3.05505
3
Total
45.7333
3.73146
15
0
45.0000
2.64575
3
1
53.0000
2.64575
3
7
58.0000
1.00000
3
14
44.6667
4.16333
3
21
46.0000
4.35890
3
Total
49.3333
6.11400
15
0
44.0000
2.64575
3
1
74.3333
2.08167
3
7
53.6667
2.51661
3
14
45.3333
3.05505
3
21
43.0000
3.00000
3
Total
52.0667
12.37778
15
0
38.6667
4.16333
3
1
75.3333
3.51188
3
7
58.0000
2.00000
3
14
43.6667
1.52753
3
21
42.0000
1.00000
3
Total
51.5333
14.27719
15
0
41.6667
3.05505
3
1
72.3333
2.51661
3
7
63.6667
2.51661
3
14
44.6667
3.78594
3
21
42.3333
4.04145
3
Total
52.9333
13.37090
15
0
42.9333
3.76955
15
1
64.5333
12.39739
15
7
56.4667
5.48852
15
14
44.4667
2.89992
15
21
43.2000
3.16679
15
Total
50.3200
10.85551
75
xcvi
Universitas Sumatera Utara
Lanjutan Lampiran 16.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Pengamatan
Type III
Sum of
Squares
Source
Corrected
Model
Intercept
F
Sig.
Partial Eta Noncent.
Squared Parameter
Observed
Powerb
8252.320a
24
343.847 36.736
.000
.946
881.658
1.000
189907.68
0
1
189907.68 2.029E
0
4
.000
.998 20289.282
1.000
500.453
4
125.113 13.367
.000
.517
53.467
1.000
151.97
1
.000
.924
607.883
1.000
128.880 13.769
.000
.815
220.308
1.000
Menit_Ke
Hari_Ke
5689.787
Menit_Ke *
Hari_Ke
Error
Total
Mean
Square
df
4 1422.447
2062.080
16
468.000
50
198628.00
0
75
9.360
Corrected
8720.320
74
Total
a. R Squared = .946 (Adjusted R Squared =
.921)
Post Hoc Tests
Lama Reaksi (menit)
Multiple Comparisons
Hasil Pengamatan
Tukey HSD
(I)
Lama
Reaksi
(menit)
0
(J)
Lama
Reaksi
(menit)
Std. Error
Sig.
Lower Bound
Upper Bound
1.11714
.018
-6.7613
-.4387
*
1.11714
.000
-9.4946
-3.1720
*
1.11714
.000
-8.9613
-2.6387
*
1.11714
.000
-10.3613
-4.0387
0
3.6000
*
1.11714
.018
.4387
6.7613
60
-2.7333
1.11714
.120
-5.8946
.4280
90
-2.2000
1.11714
.296
-5.3613
.9613
*
1.11714
.018
-6.7613
-.4387
*
1.11714
.000
3.1720
9.4946
30
2.7333
1.11714
.120
-.4280
5.8946
90
.5333
1.11714
.989
-2.6280
3.6946
120
-.8667
1.11714
.936
-4.0280
2.2946
30
90
120
120
60
Mean Difference
(I-J)
*
60
30
95% Confidence Interval
0
-3.6000
-6.3333
-5.8000
-7.2000
-3.6000
6.3333
xcvii
Universitas Sumatera Utara
Lanjutan Lampiran 16.
90
120
0
5.8000*
1.11714
.000
30
2.2000
1.11714
60
-.5333
1.11714
120
-1.4000
7.2000
*
3.6000
*
.8667
0
30
60
2.6387
8.9613
.296
-.9613
5.3613
.989
-3.6946
2.6280
1.11714
.720
-4.5613
1.7613
1.11714
.000
4.0387
10.3613
1.11714
.018
.4387
6.7613
1.11714
.936
-2.2946
4.0280
.720
-1.7613
4.5613
90
1.4000
1.11714
Based on observed means.
The error term is Mean Square(Error) = 9.360.
*. The mean difference is significant at the .05 level.
Homogeneous Subsets
Hasil Pengamatan
Tukey HSD
Lama
Reaksi
(menit)
Subset
N
1
2
3
0
15
45.7333
30
15
49.3333
90
15
51.5333
51.5333
60
15
52.0667
52.0667
120
15
52.9333
Sig.
1.000
.120
.720
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 9.360.
xcviii
Universitas Sumatera Utara
Lampiran 17. Analisis Statistik Trigliserida Pemberian Minyak Kelapa Sawit
Univariate Analysis of Variance
Descriptive Statistics
Dependent Variable:Hasil Pengamatan
Lama
Lama
Reaksi Percoba
(menit) an
0
30
60
90
120
Total
Mean
Std. Deviation
N
0
49.3333
3.51188
3
1
49.6667
3.05505
3
7
53.0000
3.60555
3
14
41.0000
3.60555
3
21
38.6667
3.05505
3
Total
46.3333
6.37704
15
0
49.0000
2.64575
3
1
55.0000
2.64575
3
7
62.0000
1.00000
3
14
41.6667
4.16333
3
21
42.0000
4.35890
3
Total
49.9333
8.50602
15
0
48.0000
2.64575
3
1
76.3333
2.08167
3
7
57.6667
2.51661
3
14
42.3333
3.05505
3
21
39.0000
3.00000
3
Total
52.6667
14.07463
15
0
42.6667
4.16333
3
1
77.3333
3.51188
3
7
62.0000
2.00000
3
14
40.6667
1.52753
3
21
38.0000
1.00000
3
Total
52.1333
15.88740
15
0
45.6667
3.05505
3
1
74.3333
2.51661
3
7
67.6667
2.51661
3
14
41.6667
3.78594
3
21
38.3333
4.04145
3
Total
53.5333
15.36167
15
0
46.9333
3.76955
15
1
66.5333
12.39739
15
7
60.4667
5.48852
15
14
41.4667
2.89992
15
21
39.2000
3.16679
15
Total
50.9200
12.57062
75
xcix
Universitas Sumatera Utara
Lanjutan Lampiran 17.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Pengamatan
Type III Sum
Source
of Squares
Corrected
Mean
df
Square
F
11225.520a
24
194463.480
1
500.453
4
125.113
8662.987
4
2062.080
Error
Total
Model
Intercept
Observed
Squared
Parameter
Powerb
1.000
.998 20776.013
1.000
13.367 .000
.517
53.467
1.000
2165.747
231.383 .000
.949
925.533
1.000
16
128.880
13.769 .000
.815
220.308
1.000
468.000
50
9.360
206157.000
75
11693.520
74
Hari_Ke
Menit_Ke
* Hari_Ke
Corrected
Total
194463.48
0
49.971 .000
Noncent.
1199.308
Menit_Ke
467.730
Sig.
Partial Eta
2.078E4 .000
.960
a. R Squared = .960 (Adjusted R Squared = .941)
b. Computed using alpha = .05
Post Hoc Tests
Lama Reaksi (menit)
Multiple Comparisons
Hasil Pengamatan
Tukey HSD
(J)
(I) Lama Lama
Reaksi Reaksi
(menit) (menit)
0
Mean Difference
(I-J)
Std. Error
Sig.
Lower Bound
Upper Bound
*
1.11714
.018
-6.7613
-.4387
*
1.11714
.000
-9.4946
-3.1720
*
1.11714
.000
-8.9613
-2.6387
*
1.11714
.000
-10.3613
-4.0387
0
3.6000
*
1.11714
.018
.4387
6.7613
60
-2.7333
1.11714
.120
-5.8946
.4280
90
-2.2000
1.11714
.296
-5.3613
.9613
*
1.11714
.018
-6.7613
-.4387
30
60
90
120
30
95% Confidence Interval
120
-3.6000
-6.3333
-5.8000
-7.2000
-3.6000
c
Universitas Sumatera Utara
Lanjutan Lampiran 17.
60
90
120
0
6.3333*
1.11714
.000
30
2.7333
1.11714
90
.5333
1.11714
120
-.8667
*
30
3.1720
9.4946
.120
-.4280
5.8946
.989
-2.6280
3.6946
1.11714
.936
-4.0280
2.2946
1.11714
.000
2.6387
8.9613
2.2000
1.11714
.296
-.9613
5.3613
60
-.5333
1.11714
.989
-3.6946
2.6280
120
-1.4000
1.11714
.720
-4.5613
1.7613
*
1.11714
.000
4.0387
10.3613
*
1.11714
.018
.4387
6.7613
.8667
1.11714
.936
-2.2946
4.0280
.720
-1.7613
4.5613
0
5.8000
0
7.2000
30
3.6000
60
90
1.4000
1.11714
Based on observed means.
The error term is Mean Square(Error) = 9.360.
*. The mean difference is significant at the .05 level.
Homogeneous Subsets
Hasil Pengamatan
Tukey HSD
Lama
Reaksi
(menit)
Subset
N
1
2
3
0
15
46.3333
30
15
49.9333
90
15
52.1333
52.1333
60
15
52.6667
52.6667
120
15
53.5333
Sig.
1.000
.120
.720
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 9.360.
ci
Universitas Sumatera Utara
Lampiran 18. Analisis Statistik HDL Pemberian Minyak Kelapa Sawit
Univariate Analysis of Variance
Dependent Variable:Hasil
Lama
Reaksi Hari_K
(Menit) e
Mean
0
0
17.3333
1.52753
3
1
16.6667
1.15470
3
7
17.3333
.57735
3
14
24.3333
1.52753
3
21
29.3333
1.52753
3
Total
21.0000
5.31843
15
0
15.0000
2.64575
3
1
15.3333
3.05505
3
7
15.3333
2.88675
3
14
21.3333
1.15470
3
21
25.0000
1.00000
3
Total
18.4000
4.64143
15
0
14.3333
2.51661
3
1
14.0000
2.00000
3
7
14.3333
2.30940
3
14
17.3333
2.30940
3
21
23.6667
2.08167
3
Total
16.7333
4.25049
15
0
14.6667
2.08167
3
1
14.0000
2.64575
3
7
13.6667
2.08167
3
14
16.0000
2.64575
3
21
21.3333
2.30940
3
Total
15.9333
3.53486
15
0
14.6667
1.52753
3
1
14.0000
1.73205
3
7
14.3333
1.52753
3
14
17.3333
2.51661
3
21
23.0000
2.00000
3
Total
16.6667
3.84831
15
0
15.2000
2.11119
15
1
14.8000
2.17781
15
7
15.0000
2.17124
15
14
19.2667
3.67359
15
21
24.4667
3.20416
15
Total
17.7467
4.62103
75
30
60
90
120
Total
Std. Deviation
N
cii
Universitas Sumatera Utara
Lanjutan Lampiran 18.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Type III Sum
Source
of Squares
Partial Eta
df
Mean Square
F
Sig.
Squared
Corrected Model
1366.187a
24
56.924
13.300
.000
.865
Intercept
23620.813
1
23620.813
5.519E3
.000
.991
247.387
4
61.847
14.450
.000
.536
1052.720
4
263.180
61.491
.000
.831
66.080
16
4.130
.965
.507
.236
Error
214.000
50
4.280
Total
25201.000
75
1580.187
74
Menit_Ke
Hari_Ke
Menit_Ke *
Hari_Ke
Corrected Total
a. R Squared = .865 (Adjusted R Squared = .800)
Post Hoc Tests
Lama Reaksi (Menit)
Multiple Comparisons
Hasil
Tukey HSD
(I)
Lama
Reaksi
(Menit)
0
(J)
Lama
Reaksi Mean Difference
(Menit)
(I-J)
Std. Error
Upper Bound
.75542
.010
.4623
4.7377
.75542
.000
2.1290
6.4044
*
.75542
.000
2.9290
7.2044
*
.75542
.000
2.1956
6.4710
*
-2.6000
.75542
.010
-4.7377
-.4623
60
1.6667
.75542
.194
-.4710
3.8044
90
*
.75542
.016
.3290
4.6044
1.7333
.75542
.164
-.4044
3.8710
*
.75542
.000
-6.4044
-2.1290
30
-1.6667
.75542
.194
-3.8044
.4710
90
.8000
.75542
.826
-1.3377
2.9377
120
.0667
.75542
1.000
-2.0710
2.2044
*
.75542
.000
-7.2044
-2.9290
*
.75542
.016
-4.6044
-.3290
60
-.8000
.75542
.826
-2.9377
1.3377
120
-.7333
.75542
.867
-2.8710
1.4044
120
0
120
90
Lower Bound
*
30
90
60
Sig.
*
60
30
95% Confidence Interval
0
0
30
2.6000
4.2667
5.0667
4.3333
2.4667
-4.2667
-5.0667
-2.4667
ciii
Universitas Sumatera Utara
Lanjutan Lampiran 18.
120
0
-4.3333*
.75542
30
-1.7333
60
-.0667
.000
-6.4710
.75542
.164
-3.8710
.4044
.75542
1.000
-2.2044
2.0710
.867
-1.4044
2.8710
90
.7333
.75542
Based on observed means.
The error term is Mean Square(Error) = 4.280.
*. The mean difference is significant at the .05 level.
-2.1956
Homogeneous Subsets
Hasil
Tukey HSD
Lama
Reaksi
(Menit)
Subset
N
1
2
3
90
15
15.9333
120
15
16.6667
16.6667
60
15
16.7333
16.7333
30
15
18.4000
0
15
21.0000
Sig.
.826
.164
1.000
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 4.280.
civ
Universitas Sumatera Utara
Lampiran 19. Analisis Statistik LDL Pemberian Minyak Kelapa Sawit
Univariate Analysis of Variance
Descriptive Statistics
Dependent Variable:Hasil
Lama
Reaksi Hari_K
(Menit) e
Mean
0
0
18.1333
3.70045
3
1
21.0667
2.89367
3
7
21.0667
3.07463
3
14
11.4667
3.13900
3
21
5.6000
2.69072
3
Total
15.4667
6.79692
15
0
20.2000
4.13280
3
1
26.6667
4.31432
3
7
30.2667
2.99555
3
14
15.0000
3.81576
3
21
12.6000
2.60000
3
Total
20.9467
7.59453
15
0
20.0667
4.60579
3
1
45.0667
3.40196
3
7
27.8000
1.38564
3
14
19.5333
1.66533
3
21
11.5333
.70238
3
Total
24.8000
11.99286
15
0
15.4667
2.91433
3
1
45.8667
4.34665
3
7
31.9333
2.30072
3
14
19.5333
2.11975
3
21
13.0667
1.66533
3
Total
25.1733
12.87142
15
0
17.8667
3.06159
3
1
43.4667
3.66788
3
7
35.8000
3.53836
3
14
19.0000
3.90384
3
21
11.6667
3.80175
3
Total
25.5600
12.79603
15
0
18.3467
3.63119
15
1
36.4267
11.26102
15
7
29.3733
5.58512
15
14
16.9067
4.20942
15
21
10.8933
3.51963
15
Total
22.3893
11.14033
75
30
60
90
120
Total
Std. Deviation
N
cv
Universitas Sumatera Utara
Lanjutan Lampiran 19.
Tests of Between-Subjects Effects
Dependent Variable:Hasil
Type III Sum
Source
of Squares
Partial Eta
df
Mean Square
F
Sig.
Squared
Corrected Model
8668.791a
24
361.200
35.060
.000
.944
Intercept
37596.169
1
37596.169
3.649E3
.000
.986
Menit_Ke
1104.295
4
276.074
26.797
.000
.682
Hari_Ke
6365.757
4
1591.439
154.473
.000
.925
1198.739
16
74.921
7.272
.000
.699
Error
515.120
50
10.302
Total
46780.080
75
9183.911
74
Menit_Ke *
Hari_Ke
Corrected Total
a. R Squared = .944 (Adjusted R Squared = .917)
Post Hoc Tests
Lama Reaksi (Menit)
Multiple Comparisons
Hasil
Tukey HSD
(I)
Lama
Reaksi
(Menit)
0
(J)
Lama
Reaksi Mean Difference
(Menit)
(I-J)
Std. Error
Lower Bound
Upper Bound
1.17203
.000
-8.7966
-2.1634
*
1.17203
.000
-12.6500
-6.0167
*
1.17203
.000
-13.0233
-6.3900
*
1.17203
.000
-13.4100
-6.7767
*
1.17203
.000
2.1634
8.7966
*
1.17203
.015
-7.1700
-.5367
90
*
-4.2267
1.17203
.006
-7.5433
-.9100
120
-4.6133*
1.17203
.002
-7.9300
-1.2967
9.3333*
1.17203
.000
6.0167
12.6500
*
1.17203
.015
.5367
7.1700
90
-.3733
1.17203
.998
-3.6900
2.9433
120
-.7600
1.17203
.966
-4.0766
2.5566
30
90
120
0
60
60
Sig.
*
60
30
95% Confidence Interval
0
30
-5.4800
-9.3333
-9.7067
-10.0933
5.4800
-3.8533
3.8533
cvi
Universitas Sumatera Utara
Lanjutan Lampiran 19.
90
9.7067*
1.17203
.000
*
1.17203
60
.3733
1.17203
120
-.3867
*
*
.7600
0
30
120
4.2267
0
10.0933
30
4.6133
60
6.3900
13.0233
.006
.9100
7.5433
.998
-2.9433
3.6900
1.17203
.997
-3.7033
2.9300
1.17203
.000
6.7767
13.4100
1.17203
.002
1.2967
7.9300
1.17203
.966
-2.5566
4.0766
.997
-2.9300
3.7033
90
.3867
1.17203
Based on observed means.
The error term is Mean Square(Error) = 10.302.
*. The mean difference is significant at the .05 level.
Homogeneous Subsets
Hasil
Tukey HSD
Lama
Reaksi
(Menit)
Subset
N
1
2
3
0
15
15.4667
30
15
20.9467
60
15
24.8000
90
15
25.1733
120
15
25.5600
Sig.
1.000
1.000
.966
Means for groups in homogeneous subsets are displayed.
Based on observed means.
The error term is Mean Square(Error) = 10.302.
cvii
Universitas Sumatera Utara
Lampiran 20. Pengujian Beda Nilai Rata-Rata Kadar Kolesterol Total Setelah
Pemberian Lemak Sapi dan Minyak Kelapa Sawit Hasil
Interesterifikasi
Rata-rata Kadar (n=3) Kolesterol total (mg/dl)
Hari keLemak Sapi
Minyak Kelapa Sawit
0
44.67
44.00
1
83.67
74.33
7
94.67
53.67
14
61.33
45.33
21
58.67
43.00
Total
343.01
260.33
Mean
X a = 68.60
X b = 52.07
St. Deviasi
STD a = 20.19
STD b = 13.14
Kedua sampel adalah independen, maka dilakukan uji t beda antara dua mean dengan
taraf kepercayaan 95% untuk mengetahui apakah rata-rata kedua sampel sama (σ 1 =
σ 2 ) atau berbeda (σ 1 ≠ σ 2 ) .
1. H o : σ 1 = σ 2
H1 : σ 1 ≠ σ 2
2. Nilai kritis t yang diperoleh dari tabel (t 0,05/2 (8))adalah = 2,3060
Daerah kritis penerimaan : -2,3060 ≤ t o ≤ 2,3060
Daerah kritis penolakan
: t o < -2,3060 dan t o > 2,3060
3. T hitung antara dua mean
cviii
Universitas Sumatera Utara
Lanjutan Lampiran 20.
4. Karena t o = 2.81 > 2.3060, maka hipotesa H o ditolak. Berarti terdapat
perbedaan signifikan rata-rata kadar kolesterol total lemak sapi dan minyak
kelapa sawit hasil interesterifikasi.
cix
Universitas Sumatera Utara
Lampiran 21. Pengujian Beda Nilai Rata-Rata Kadar Trigliserida Setelah Pemberian
Lemak Sapi dan Minyak Kelapa Sawit Hasil Interesterifikasi
Rata-rata Kadar (n=3) Trigliserida (mg/dl)
Hari keLemak Sapi
Minyak Kelapa Sawit
0
48.67
48.00
1
85.67
76.33
7
98.67
57.67
14
58.33
42.33
21
54.67
39.00
Total
346.01
263.33
Mean
X a = 69.20
X b = 52.67
St. Deviasi
STD a = 21.74
STD b = 15.00
Kedua sampel adalah independen, maka dilakukan uji t beda antara dua mean dengan
taraf kepercayaan 95% untuk mengetahui apakah rata-rata kedua sampel sama (σ 1 =
σ 2 ) atau berbeda (σ 1 ≠ σ 2 ) .
1. H o : σ 1 = σ 2
H1 : σ 1 ≠ σ 2
2. Nilai kritis t yang diperoleh dari tabel (t 0,05/2 (8))adalah = 2,3060
Daerah kritis penerimaan : -2,3060 ≤ t o ≤ 2,3060
Daerah kritis penolakan
: t o < -2,3060 dan t o > 2,3060
3. T hitung antara dua mean
cx
Universitas Sumatera Utara
Lanjutan Lampiran 21.
4. Karena t o = 2.55 > 2.3060, maka hipotesa H o ditolak. Berarti terdapat
perbedaan signifikan rata-rata kadar trigliserida lemak sapi dan minyak kelapa
sawit hasil interesterifikasi.
cxi
Universitas Sumatera Utara
Lampiran 22. Pengujian Beda Nilai Rata-Rata Kadar HDL Setelah Pemberian
Lemak Sapi dan Minyak Kelapa Sawit Hasil Interesterifikasi
Rata-rata Kadar (n=3) HDL (mg/dl)
Hari keLemak Sapi
Minyak Kelapa Sawit
0
14.67
14.33
1
14.00
14.00
7
14.33
14.33
14
14.00
17.33
21
14.67
23.67
Total
71.67
83.66
Mean
X a = 14.33
X b = 16.73
St. Deviasi
STD a = 0.34
STD b = 4.11
Kedua sampel adalah independen, maka dilakukan uji t beda antara dua mean dengan
taraf kepercayaan 95% untuk mengetahui apakah rata-rata kedua sampel sama (σ 1 =
σ 2 ) atau berbeda (σ 1 ≠ σ 2 ) .
1. H o : σ 1 = σ 2
H1 : σ 1 ≠ σ 2
2. Nilai kritis t yang diperoleh dari tabel (t 0,05/2 (8))adalah = 2,3060
Daerah kritis penerimaan : -2,3060 ≤ t o ≤ 2,3060
Daerah kritis penolakan
: t o < -2,3060 dan t o > 2,3060
3. T hitung antara dua mean
cxii
Universitas Sumatera Utara
Lanjutan Lampiran 22.
4. Karena t o = 3.05 > 2.3060, maka hipotesa H o ditolak. Berarti terdapat
perbedaan signifikan rata-rata kadar HDL lemak sapi dan minyak kelapa sawit
hasil interesterifikasi.
cxiii
Universitas Sumatera Utara
Lampiran 23. Pengujian Beda Nilai Rata-Rata Kadar LDL Setelah Pemberian
Lemak Sapi dan Minyak Kelapa Sawit Hasil Interesterifikasi
Rata-rata Kadar (n=3) LDL (mg/dl)
Hari keLemak Sapi
Minyak Kelapa Sawit
0
20.27
20.07
1
52.53
45.07
7
60.60
27.80
14
35.67
19.53
21
33.06
11.53
Total
202.13
124.00
Mean
X a = 40.43
X b = 24.80
St. Deviasi
STDa =16.10
STD b = 12.71
Kedua sampel adalah independen, maka dilakukan uji t beda antara dua mean dengan
taraf kepercayaan 95% untuk mengetahui apakah rata-rata kedua sampel sama (σ 1 =
σ 2 ) atau berbeda (σ 1 ≠ σ 2 ) .
1. H o : σ 1 = σ 2
H1 : σ 1 ≠ σ 2
2. Nilai kritis t yang diperoleh dari tabel (t 0,05/2 (8))adalah = 2,3060
Daerah kritis penerimaan : -2,3060 ≤ t o ≤ 2,3060
Daerah kritis penolakan
: t o < -2,3060 dan t o > 2,3060
3. T hitung antara dua mean
cxiv
Universitas Sumatera Utara
Lanjutan Lampiran 23.
4. Karena t o = 3.07 > 2.3060, maka hipotesa H o ditolak. Berarti terdapat
perbedaan signifikan rata-rata kadar LDL lemak sapi dan minyak kelapa sawit
hasil interesterifikasi.
cxv
Universitas Sumatera Utara
Lampiran 24. Tabel Nilai Kritik Distribusi t
cxvi
Universitas Sumatera Utara
Lampiran 25. Gambar Alat
Microlab 300 (Vital Scientific)
Melting Point Aparatus (Stuart)
cxvii
Universitas Sumatera Utara
Lampiran 26. Surat Keterangan Pemakaian Laboratorium Kesehatan Dinas
Kesehatan Propinsi Sumatera Utara
cxviii
Universitas Sumatera Utara
Lampiran 27. Surat Keterangan Pemakaian Laboratorium Penelitian Fakultas
Farmasi USU
cxix
Universitas Sumatera Utara
Lampiran 28. Etichal Clearance Komite Etik Penelitian Hewan
cxx
Universitas Sumatera Utara