69 LAMPIRAN A SURAT DETERMINASI TANAMAN PUTRI MALU
LAMPIRAN A
SURAT DETERMINASI TANAMAN PUTRI MALU
69
LAMPIRAN B
SERTIFIKAT HEWAN COBA
70
LAMPIRAN C
SERTIFIKAT KODE ETIK
71
LAMPIRAN D
DASAR PENGGUNAAN DOSIS
Dalam penelitian ini penggunaan dosis ditingkatkan berdasarkan
faktor pengali 3,2. Untuk menentukan faktor pengali dapat diketahui dengan
rumus sebagai berikut:
F = faktor pengali
F= √
n = jumlah dosis rentang -1
I=
I=
= 9,09 ~ 9,1
F = √9,1
= x log 9,1
= x 0,96
= 0,48 ~ 0,5
= anti log 0,5
= 3,16 ~ 3,2
Dosis 1 = 550 mg/kg BB
Dosis 2 = 550 x 3,2 = 1760 mg/kg BB, (dosis yang dipakai 1750 mg/kg BB)
Dosis 3 = 1750 x 3,2 = 5600 mg/kg BB, (dosis yang dipakai 5000 mg/kg
BB)
72
LAMPIRAN E
HASIL PERHITUNGAN KADAR ABU SIMPLISIA
No
1.
2.
3.
I.
W (krus
kosong)
(gram)
33,4309
33,9405
32,6390
W
(bahan)
(gram)
2,0077
2,0053
2,0087
Kadar Abu =
(
)
=
II.
Kadar Abu =
,
,
)
,
,
(
)
=
Rata – Rata =
,
,
,
,
= 4,44%
73
4,44% ± 0,135
× 100%
× 100%
× 100%
,
,
Rata – rata ±
SD
× 100% = 4,30%
,
Kadar Abu =
%
Kadar
Abu
4,57
4,30
4,45
× 100% = 4,57%
,
(
=
III.
W (krus
+ abu)
(gram)
33,5226
33,0268
32,7283
× 100% = 4,45
LAMPIRAN F
HASIL PERHITUNGAN KADAR AIR SIMPLISIA
No.
W (cawan W (bahan)W (cawan + % Kadar Rata – rata± SD
kosong)
(gram)
simplisia)
(gram)
Air
(gram)
1.
119,0803
2,0119
120,9250
8,31%
2.
85,2373
2,0049
87,0713
8,49%
I.
–
Kadar Air =
=
,
,
–
=
,
× 100%
× 100% = 8,31%
,
II. Kadar Abu =
Rata – Rata =
8,40%± 0,127
,
,
,
,
= 8,40%
74
× 100%
× 100% = 8,49%
LAMPIRAN G
HASIL PERHITUNGAN KADAR SARI LARUT AIR SIMPLISIA
No.
W (Cawan W (bahan)
kosong)
W
% Kadar Sari Rata – rata± SD
(gram)
Larut
(cawan +
(gram)
Etanol
filtrat)
(gram)
1.
83,5523
5,0037
83,7050
15,26%
2.
79,1089
5,0036
79,2652
15,62%
3.
57,3892
5,0052
57,5441
15,47%
1. Kadar Sari Larut Etanol =
(
=
2.Kadar Sari Larut Etanol =
)–
,
,
(
,
,
(
)–
3.Kadar Sari Larut Etanol =
,
=
,
× 100%
×
,
,
,
,
,
= 15,45%
75
× 100%
× 100% = 15,26%
)–
=
Rata – Rata =
×
,
15,45% ± 0,18
× 100% = 15,62%
× 100%
×
× 100% = 15,47%
LAMPIRAN H
HASIL PERHITUNGAN KADAR SARI LARUT ETANOL SIMPLISIA
No. W (CawanW (bahan)
kosong)
W
% Kadar Sari Rata – rata± SD
(gram) (cawan +
(gram)
Larut Air
filtrat)
(gram)
1.
83,5494
5,0028
83,6828
13,33%
2.
83,1287
5,0079
83,2676
13,87%
1.Kadar Sari Larut Air =
(
)–
=
2.Kadar Sari Larut Air =
,
×
,
(
)–
=
Rata – Rata =
,
,
,
,
×
,
,
= 13,60%
76
13,60%± 0,38
× 100%
× 100% = 13,33 %
× 100%
× 100% = 13,87 %
77
LAMPIRAN I
SKRINING FITOKIMIA
A.
B.
(D)
C.
(E)
Keterangan :
A. Alkaloid dengan pereaksi dragendroff
B. Flavonoid dengan penambahan serbuk Mg, 2 ml alkohol
khlorhidrik, dan amil alkohol
C. Saponin
D. Tanin dengan pereaksi FeCL3 dan larutan gelatin
E. Terpenoid atau steroid
78
LAMPIRAN J
HASIL PERHITUNGAN KADAR ABU EKSTRAK
No
1.
2.
I.
W (krus
kosong)
(gram)
31,7720
33,3549
W
(bahan)
(gram)
2,0009
2,0052
Kadar Abu =
(
)
=
II.
W (krus
+ abu)
(gram)
31,8652
33,4415
,
,
(
)
=
Rata – Rata =
,
,
,
= 4,48%
79
4,48% ± 0,226
× 100%
× 100%
,
,
Rata – rata ±
SD
× 100% = 4,64%
,
Kadar Abu =
%
Kadar
Abu
4,64%
4,32%
× 100% = 4,32%
LAMPIRAN K
HASIL PERHITUNGAN KADAR ABU TIDAK LARUT AIR
No
1.
2.
I.
W
(krus
kosong)
(gram)
Bobot
abu
awal
(gram)
33,3549
31,7720
2,0052
2,0009
W
(Krus
+
bahan)
(gram)
33,4415
31,8652
Kadar Abu =
(
=
II.
Kadar Abu =
,
,
%
Kadar
Abu
Larut
Air
3,68%
3,34%
)
,
,
,
,
× 100%
× 100% = 3,34%
= 3,51%
\
80
3,51% ±0,24
× 100%
)
,
Rata – rata ±
SD
× 100% = 3,68%
,
(
=
Rata – Rata =
W
(Krus +
kertas
saring)
(gram)
33,3677
31,7983
LAMPIRAN L
HASIL PERHITUNGAN KADAR ABU TIDAK LARUT ASAM
No
W
(krus
kosong)
(gram)
Bobot
abu
awal
(gram)
1.
2.
32,1937
31,7524
2,0097
2,0087
I.
W
(Krus
+
bahan)
(gram)
32,2276
31,7941
Kadar Abu =
32,2013
31,7629
(
)
=
II.
W (Krus
+ kertas
saring)
(gram)
,
,
(
)
Rata – Rata =
,
,
,
Rata – rata ±
SD
1,43% ±0,170
× 100%
× 100% = 1,31%
,
Kadar Abu =
=
%
Kadar
Abu
Larut
Air
1,31%
1,55%
,
,
= 1,43%
81
× 100%
× 100% = 1,55%
LAMPIRAN M
HASIL PERHITUNGAN KADAR AIR EKSTRAK
No. W (cawan W (Zat) W (cawan +% Kadar Rata – rata± SD
kosong)
(gram)
simplisia)
(gram)
Air
(gram)
1.
83,5648
5,0054
88,1658
8,01%
2.
88,1440
5,0012
92,7140
8,62%
3.
79,0626
5,0051
83,6179
8,98%
I.
× 100%
Kadar Air =
,
=
II.
,
× 100% = 8,01%
,
× 100%
Kadar Abu =
,
=
III.
8,54%± 0,490
,
× 100% = 8,62%
,
× 100%
Kadar Abu
,
=
Rata – Rata =
,
,
,
,
,
= 8,54%
82
× 100% = 8,98%
LAMPIRAN N
PERHITUNGAN PENIMBANGAN EKSTRAK
Berat Badan Sebelum Perlakuan (gram)
Kelompok Kelompok Perlakuan 1 elompok Perlakuan 1 Kelompok Perlakuan 1
Kontrol
(Dosis 550
(Dosis 1750
(Dosis 5000 mg/kgBB)
mg/kgBB)
mg/kgBB)
26
21
33
28
29
29
28
27
28
26
29
36
17
32
29
33
Berikut adalah perhitungan penimbangan ekstrak untuk mencit jantan
galur Swiss :
Vp =
(
)
(
/
)
,
Keterangan :
Vp : Faktor Pengenceran
D
: Dosis
C
: Konsentrasi,
berdasarkan rumus diatas maka dapat dihitung :
Dosis 550 mg/kgBB :
Vp(2,5ml)=
C=
× 550 : C = 59,4 mg = 0,0594 gram
0,0594 gram
2 ml
= 0,0297 gram/ ml X 5 ml = 0,1485 gram/ 5ml
Dosis 1750 mg/kgBB :
Vp(2,5ml)=
C=
× 1750 : C = 208, 25mg = 0,20825 gram
0,20825 gram
2 ml
= 0,1041 gram/ ml X 5 ml = 0,5206 gram/ 5ml
Dosis 5000 mg/kgBB :
Vp(2,5ml)=
C=
× 5000 : C = 620 mg = 0,62 gram
0,62 gram
2 ml
= 0,31 gram/ ml X 5 ml = 1,55 gram/ 5ml
83
LAMPIRAN O
PENIMBANGAN ORGAN (gram)
Kode
BB
awal
BB akhir
Bobot Organ (gram)
Jantung
Ginjal
Paru
Testis
Limfa
Hati
Vas. Differens
84
Kontrol 1
16
28
0,16
0,48
0,34
0,28
0,42
2,16
0,22
Kontrol 2
17
31
0,14
0,43
0,23
0,23
0,19
1,48
0,22
Kontrol 3
17
31
0,16
0,61
0,29
0,31
0,26
1,99
0,26
Kontrol 4
15
21
0,14
0,23
0,27
0,28
0,24
1,82
0,26
Dosis 550 mg/kgBB
16
25
0,09
0,34
0,19
0,24
0,25
1,31
0,15
Dosis 550 mg/kgBB
17
31
0,14
0,45
0,25
0,28
0,31
2,07
0,38
Dosis 550 mg/kgBB
16
32
0,16
0,33
0,35
0,34
0,13
1,51
0,27
Dosis 550 mg/kgBB
19
34
0,16
0,48
0,24
0,24
0,15
1,44
0,18
Dosis 1750 mg/kgBB
20
35
0,15
0,48
0,22
0,34
0,24
2,05
0,37
Dosis 1750 mg/kgBB
17
30
0,14
0,50
0,24
0,26
0,24
0,94
0,16
Dosis 1750 mg/kgBB
18
33
0,13
0,51
0,25
0,25
0,48
1,75
0,21
Dosis 1750 mg/kgBB
18
32
0,11
0,38
0,31
0,23
0,13
1,39
0,27
Dosis 5000 mg/kgBB
17
29
0,16
0,49
0,26
0,29
0,22
1,84
0,36
Dosis 5000 mg/kgBB
17
29
0,12
0,44
0,21
0,23
0,22
1,75
0,19
Dosis 5000 mg/kgBB
20
34
0,14
0,46
0,32
0,23
0,26
1,76
0,31
Dosis 5000 mg/kgBB
19
34
0,13
0,48
0,24
0,27
0,16
1,49
0,35
85
LAMPIRAN P
PERHITUNGAN INDEKS ORGAN (%)
Jantung
Ginjal
0,16
ontrol 1
Paru
0,48
× 100
28
= 0,57
0,34
× 100
28
= 1,21
28
× 100
= 1,71
Testis
Limpa
0,28
0,42
× 100
28
= 1,00
Hati
Vas Differens
2,16
× 100
28
= 1,50
0,22
× 100
28
= 0,79
28
× 100
= 7,71
86
ontrol 2
45
39
74
74
61
77
71
ontrol 3
52
97
94
00
84
42
84
ontrol 4
67
95
29
33
14
67
24
0,36
1,36
0,76
0,96
1,00
5,24
0,60
Dosis 550 mg/kgBB
0,45
1,45
0,81
0,90
1,00
6,67
1,22
Dosis 550 mg/kgBB
0,50
1,22
1,30
1,26
0,48
5,59
1,00
Dosis 550 mg/kgBB
0,47
1,41
0,71
0,70
0,44
4,24
0,53
0,43
1,37
0,63
0,97
0,69
5,86
1,06
Dosis 1750 mg/kgBB
0,47
1,67
0,80
0,87
0,80
3,13
0,53
Dosis 1750 mg/kgBB
0,39
1,55
0,76
0,76
1,45
5,30
0,64
osis 550 mg/kgBB
osis 1750 mg/kgBB
0,34
Dosis 1750 mg/kgBB
1,19
0,97
0,72
0,41
4,34
0,84
55
69
90
00
76
34
24
Dosis 5000 mg/kgBB
41
52
72
79
76
03
66
Dosis 5000 mg/kgBB
41
35
94
68
76
18
91
Dosis 5000 mg/kgBB
38
41
71
79
47
38
03
osis 5000 mg/kgBB
87
LAMPIRAN Q
HASIL PENGAMATAN KELOMPOK KONTROL
Keterangan :
+
: Ada Aktivitas
: Tidak Ada Aktivitas
Kj
: Kuning Jernih
P
: Padat
88
89
LAMPIRAN R
HASIL PENGAMATAN KELOMPOK PERLAKUAN DOSIS 550 mg/kgBB
90
91
LAMPIRAN S
HASIL PENGAMATAN KELOMPOK PERLAKUAN DOSIS 1750 mg/kgBB
92
93
LAMPIRAN T
HASIL PENGAMATAN KELOMPOK PERLAKUAN DOSIS 5000 mg/kgBB
94
95
LAMPIRAN U
ORGAN KELOMPOK KONTROL DAN KELOMPOK PERLAKUAN
Kontrol 1
Kontrol 2
96
Kontrol 3
Kontrol 4
97
Dosis 550 mg/kgBB (5)
Dosis 550 mg/kgBB (6)
98
Dosis 550 mg/kgBB (7)
Dosis 550 mg/kgBB (8)
99
Dosis 1750 mg/kgBB (9)
Dosis 1750 mg/kgBB (10)
100
Dosis 1750 mg/kgBB (11)
Dosis 1750 mg/kgBB (12)
101
Dosis 5000 mg/kgBB (13)
Dosis 5000 mg/kgBB (14)
102
Dosis 5000 mg/kgBB (15)
Dosis 5000 mg/kgBB (16)
103
LAMPIRAN V
LAMBUNG KELOMPOK KONTROL DAN KELOMPOK
PERLAKUAN
Kontrol
Perlakuan Dosis 550 mg/kgBB
Perlakuan Dosis 1750 mg/kgBB
Perlakuan Dosis 5000 mg/kgBB
104
LAMPIRAN W
PERHITUNGAN JUMLAH JENGUKAN DENGAN ONE WAY ANOVA
Test of Homogeneity of Variances
Jumlah_Jengukan
Levene Statistic
df1
df2
1.556
3
Sig.
12
.251
ANOVA
Jumlah_Jengukan
Sum of
Squares
Between Groups
Within Groups
Total
Df
8.188
50.250
58.438
Mean Square
3
12
15
F
Sig.
2.729 .652 .597
4.188
Test of Homogeneity of Variances
Jumlah_jengukan
Levene Statistic
df1
7.643
df2
Sig.
3
12
.004
ANOVA
Jumlah_jengukan
Sum of
Squares
Between Groups
Within Groups
Total
10.188
37.750
47.938
Df
3
12
15
Mean Square
3.396
3.146
Test of Homogeneity of Variances
102
F
1.079
Sig.
.395
Jumlah_Jengukan
Levene Statistic
df1
df2
.770
3
Sig.
12
.533
Mean
Square
F
ANOVA
Jumlah_Jengukan
Sum of Squares
Between Groups
Within Groups
Total
Df
15.500
23.500
39.000
3
12
15
Sig.
5.167 2.638 .097
1.958
Test of Homogeneity of Variances
Jumlah_Jengukan
Levene Statistic
.404
df1
df2
3
Sig.
12
.753
ANOVA
Jumlah_Jengukan
Sum of Squares
Mean
Square
Df
Between Groups
29.500
3
Within Groups
Total
11.500
41.000
12
15
103
F
9.833 10.26
1
.958
Sig.
.001
Post Hoc Tests
Multiple Comparisons
Jumlah_Jengukan
Tukey HSD
(I) Parameter
(J) Parameter
Kontrol
Dosis 550 mg/kgBB
Mean
Difference (I-J)
Dosis 1750 mg/kgBB
Dosis 5000 mg/kgBB
Dosis 550
mg/kgBB
104
Dosis 1750
mg/kgBB
Dosis 5000
mg/kgBB
Kontrol
95% Confidence Interval
Std. Error
Sig.
Lower Bound
Upper Bound
-.750
.692
.706
-2.81
*
.692
.016
-4.56
-.44
1.250
.692
.317
-.81
3.31
2.81
-2.500
1.31
.750
.692
.706
-1.31
Dosis 1750 mg/kgBB
-1.750
.692
.105
-3.81
.31
Dosis 5000 mg/kgBB
2.000
.692
.057
-.06
4.06
2.500*
.692
.016
.44
4.56
Dosis 550 mg/kgBB
1.750
.692
.105
-.31
3.81
Dosis 5000 mg/kgBB
3.750*
.692
.001
1.69
5.81
Kontrol
-1.250
.692
.317
-3.31
.81
Dosis 550 mg/kgBB
-2.000
.692
.057
-4.06
.06
Dosis 1750 mg/kgBB
-3.750*
.692
.001
-5.81
-1.69
Kontrol
*. The mean difference is significant at the 0.05 level.
Homogeneous Subsets
Jumlah_Jengukan
Tukey HSDa
Subset for alpha = 0.05
Parameter
N
1
2
Dosis 5000 mg/kgBB
4
1.50
Kontrol
4
2.75
Dosis 550 mg/kgBB
4
3.50
Dosis 1750 mg/kgBB
4
Sig.
.057
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 4,000.
3.50
5.25
.105
Test of Homogeneity of Variances
Jumlah_Jengukan
Levene Statistic
df1
1.493
df2
Sig.
3
12
.266
ANOVA
Jumlah_Jengukan
Sum of
Squares
Between Groups
Within Groups
Total
23.500
36.500
60.000
Df
Mean Square
3
12
15
105
F
7.833 2.575
3.042
Sig.
.103
Test of Homogeneity of Variances
Jumlah_Jengukan
Levene Statistic
df1
df2
1.338
Sig.
3
12
.308
ANOVA
Jumlah_Jengukan
Sum of
Squares
Between Groups
Within Groups
Total
Mean
Square
df
32.188
57.250
89.438
3
12
15
F
10.729
4.771
Sig.
2.249
.135
Test of Homogeneity of Variances
Jumlah_Jengukan
Levene Statistic
df1
2.267
df2
3
Sig.
12
.133
ANOVA
Jumlah_Jengukan
Sum of
Squares
Between Groups
Within Groups
Total
13.688
72.750
86.438
Mean
Square
Df
3
12
15
106
4.563
6.063
F
.753
Sig.
.542
Test of Homogeneity of Variances
Jumlah_Jengukan
Levene Statistic
df1
1.811
df2
3
Sig.
12
.199
ANOVA
Jumlah_Jengukan
Sum of
Squares
Between Groups
Within Groups
Total
7.188
42.250
49.438
Mean
Square
Df
3
12
15
107
2.396
3.521
F
.680
Sig.
.581
LAMPIRAN X
PERHITUNGAN WAKTU MENGGELANTUNG DENGAN ONE WAY
ANOVA
Test of Homogeneity of Variances
Waktu_Menggelantung
Levene Statistic
df1
df2
5.697
Sig.
3
12
.012
ANOVA
Waktu_Menggelantung
Sum of
Squares
Between
Groups
Within Groups
Total
Mean
Square
df
72.610
3
24.203
258.031
330.640
12
15
21.503
F
Sig.
1.126
.378
Test of Homogeneity of Variances
Waktu_Menggelantung
Levene Statistic
df1
34.762
df2
Sig.
3
12
.000
ANOVA
Waktu_Menggelantung
Sum of
Squares
Between Groups
Within Groups
Total
241.470
625.710
867.180
df
Mean Square
3
12
15
108
80.490
52.142
F
1.544
Sig.
.254
Test of Homogeneity of Variances
Waktu_menggelantung
Levene Statistic
df1
df2
.492
Sig.
3
12
.694
ANOVA
Waktu_menggelantung
Sum of
Squares
Between Groups
Within Groups
Total
Mean
Square
Df
82.228
820.385
902.613
3
12
15
F
27.409
68.365
Sig.
.401
.755
Test of Homogeneity of Variances
Waktu_Menggelantung
Levene Statistic
df1
df2
.617
3
Sig.
12
.617
Mean
Square
F
ANOVA
Waktu_Menggelantung
Sum of
Squares
Between Groups
Within Groups
Total
8.383
489.224
497.607
Df
3 2.794
12 40.769
15
109
.069
Sig.
.976
Test of Homogeneity of Variances
Waktu_Menggelantung
Levene Statistic
df1
6.000
df2
3
Sig.
12
.010
ANOVA
Waktu_Menggelantung
Sum of
Squares
Between
Groups
Within Groups
Total
Mean
Square
df
F
30.583
3
10.194
183.493
214.076
12
15
15.291
Sig.
.667
.588
Test of Homogeneity of Variances
Waktu_Menggelantung
Levene Statistic
df1
3.882
df2
3
Sig.
12
.038
ANOVA
Waktu_Menggelantung
Sum of
Squares
Between Groups
Within Groups
Total
57.756
535.608
593.364
Mean
Square
df
3
12
15
F
19.252
44.634
Test of Homogeneity of Variances
Waktu_Menggelantung
Levene Statistic
3.747
df1
df2
3
Sig.
12
110
.041
.431
Sig.
.734
ANOVA
Waktu_Menggelantung
Sum of
Squares
Between Groups
Df
Mean Square
60.182
3
20.061
Within Groups
578.158
12
48.180
Total
638.339
15
F
Sig.
.416
.744
Test of Homogeneity of Variances
Waktu_Menggelantung
Levene Statistic
df1
.711
df2
Sig.
3
12
.564
ANOVA
Waktu_Menggelantung
Sum of
Squares
Between
Groups
Within Groups
Total
Df
Mean Square
208.195
3
69.398
1097.507
1305.701
12
15
91.459
111
F
.759
Sig.
.538
LAMPIRAN Y
PERHITUNGAN INDEKS ORGAN DENGAN ONE WAY ANOVA
Test of Homogeneity of Variances
Organ_Ginjal
Levene Statistic
df1
.573
df2
3
Sig.
12
.644
ANOVA
Organ_Ginjal
Sum of
Squares
Between Groups
Within Groups
Total
Mean
Square
Df
.408
.525
.933
3
12
15
F
.136
.044
3.109
Sig.
.067
Test of Homogeneity of Variances
Organ_Hati
Levene Statistic
df1
.432
df2
3
Sig.
12
.734
ANOVA
Organ_Hati
Sum of
Squares
Between Groups
Within Groups
Total
9.453
17.812
27.265
Mean
Square
Df
3
12
15
112
3.151
1.484
F
2.123
Sig.
.151
Test of Homogeneity of Variances
Organ_Jantung
Levene Statistic
df1
df2
.321
3
Sig.
12
.810
ANOVA
Organ_Jantung
Sum of
Squares
Between Groups
Within Groups
Total
df
.046
.062
.108
Mean
Square
3
12
15
F
.015 2.990
.005
Sig.
.073
Test of Homogeneity of Variances
Organ_Limpa
Levene Statistic
df1
1.367
df2
3
Sig.
12
.300
ANOVA
Organ_Limpa
Sum of
Squares
Between Groups
Within Groups
Total
Mean
Square
df
.281
1.421
1.702
3
12
15
F
.094
.118
.792
Test of Homogeneity of Variances
Organ_Paru
Levene Statistic
1.711
df1
df2
3
Sig.
12
113
.218
Sig.
.521
ANOVA
Organ_Paru
Sum of
Squares
Between Groups
Within Groups
Total
Mean
Square
df
.163
.378
.541
3
12
15
F
.054
.032
1.724
Sig.
.215
Test of Homogeneity of Variances
Organ_Testis
Levene Statistic
df1
df2
.330
3
Sig.
12
.804
ANOVA
Organ_Testis
Sum of
Squares
Between Groups
Within Groups
Total
Mean
Square
df
.121
.301
.422
3
12
15
F
.040
.025
Test of Homogeneity of Variances
Organ_vasDifferen
Levene Statistic
.185
df1
df2
3
Sig.
12
114
.904
1.611
Sig.
.239
ANOVA
Organ_vasDifferen
Sum of
Squares
Between Groups
Within Groups
Total
Mean
Square
df
.094
.797
.892
3
12
15
115
.031
.066
F
.473
Sig.
.707
SURAT DETERMINASI TANAMAN PUTRI MALU
69
LAMPIRAN B
SERTIFIKAT HEWAN COBA
70
LAMPIRAN C
SERTIFIKAT KODE ETIK
71
LAMPIRAN D
DASAR PENGGUNAAN DOSIS
Dalam penelitian ini penggunaan dosis ditingkatkan berdasarkan
faktor pengali 3,2. Untuk menentukan faktor pengali dapat diketahui dengan
rumus sebagai berikut:
F = faktor pengali
F= √
n = jumlah dosis rentang -1
I=
I=
= 9,09 ~ 9,1
F = √9,1
= x log 9,1
= x 0,96
= 0,48 ~ 0,5
= anti log 0,5
= 3,16 ~ 3,2
Dosis 1 = 550 mg/kg BB
Dosis 2 = 550 x 3,2 = 1760 mg/kg BB, (dosis yang dipakai 1750 mg/kg BB)
Dosis 3 = 1750 x 3,2 = 5600 mg/kg BB, (dosis yang dipakai 5000 mg/kg
BB)
72
LAMPIRAN E
HASIL PERHITUNGAN KADAR ABU SIMPLISIA
No
1.
2.
3.
I.
W (krus
kosong)
(gram)
33,4309
33,9405
32,6390
W
(bahan)
(gram)
2,0077
2,0053
2,0087
Kadar Abu =
(
)
=
II.
Kadar Abu =
,
,
)
,
,
(
)
=
Rata – Rata =
,
,
,
,
= 4,44%
73
4,44% ± 0,135
× 100%
× 100%
× 100%
,
,
Rata – rata ±
SD
× 100% = 4,30%
,
Kadar Abu =
%
Kadar
Abu
4,57
4,30
4,45
× 100% = 4,57%
,
(
=
III.
W (krus
+ abu)
(gram)
33,5226
33,0268
32,7283
× 100% = 4,45
LAMPIRAN F
HASIL PERHITUNGAN KADAR AIR SIMPLISIA
No.
W (cawan W (bahan)W (cawan + % Kadar Rata – rata± SD
kosong)
(gram)
simplisia)
(gram)
Air
(gram)
1.
119,0803
2,0119
120,9250
8,31%
2.
85,2373
2,0049
87,0713
8,49%
I.
–
Kadar Air =
=
,
,
–
=
,
× 100%
× 100% = 8,31%
,
II. Kadar Abu =
Rata – Rata =
8,40%± 0,127
,
,
,
,
= 8,40%
74
× 100%
× 100% = 8,49%
LAMPIRAN G
HASIL PERHITUNGAN KADAR SARI LARUT AIR SIMPLISIA
No.
W (Cawan W (bahan)
kosong)
W
% Kadar Sari Rata – rata± SD
(gram)
Larut
(cawan +
(gram)
Etanol
filtrat)
(gram)
1.
83,5523
5,0037
83,7050
15,26%
2.
79,1089
5,0036
79,2652
15,62%
3.
57,3892
5,0052
57,5441
15,47%
1. Kadar Sari Larut Etanol =
(
=
2.Kadar Sari Larut Etanol =
)–
,
,
(
,
,
(
)–
3.Kadar Sari Larut Etanol =
,
=
,
× 100%
×
,
,
,
,
,
= 15,45%
75
× 100%
× 100% = 15,26%
)–
=
Rata – Rata =
×
,
15,45% ± 0,18
× 100% = 15,62%
× 100%
×
× 100% = 15,47%
LAMPIRAN H
HASIL PERHITUNGAN KADAR SARI LARUT ETANOL SIMPLISIA
No. W (CawanW (bahan)
kosong)
W
% Kadar Sari Rata – rata± SD
(gram) (cawan +
(gram)
Larut Air
filtrat)
(gram)
1.
83,5494
5,0028
83,6828
13,33%
2.
83,1287
5,0079
83,2676
13,87%
1.Kadar Sari Larut Air =
(
)–
=
2.Kadar Sari Larut Air =
,
×
,
(
)–
=
Rata – Rata =
,
,
,
,
×
,
,
= 13,60%
76
13,60%± 0,38
× 100%
× 100% = 13,33 %
× 100%
× 100% = 13,87 %
77
LAMPIRAN I
SKRINING FITOKIMIA
A.
B.
(D)
C.
(E)
Keterangan :
A. Alkaloid dengan pereaksi dragendroff
B. Flavonoid dengan penambahan serbuk Mg, 2 ml alkohol
khlorhidrik, dan amil alkohol
C. Saponin
D. Tanin dengan pereaksi FeCL3 dan larutan gelatin
E. Terpenoid atau steroid
78
LAMPIRAN J
HASIL PERHITUNGAN KADAR ABU EKSTRAK
No
1.
2.
I.
W (krus
kosong)
(gram)
31,7720
33,3549
W
(bahan)
(gram)
2,0009
2,0052
Kadar Abu =
(
)
=
II.
W (krus
+ abu)
(gram)
31,8652
33,4415
,
,
(
)
=
Rata – Rata =
,
,
,
= 4,48%
79
4,48% ± 0,226
× 100%
× 100%
,
,
Rata – rata ±
SD
× 100% = 4,64%
,
Kadar Abu =
%
Kadar
Abu
4,64%
4,32%
× 100% = 4,32%
LAMPIRAN K
HASIL PERHITUNGAN KADAR ABU TIDAK LARUT AIR
No
1.
2.
I.
W
(krus
kosong)
(gram)
Bobot
abu
awal
(gram)
33,3549
31,7720
2,0052
2,0009
W
(Krus
+
bahan)
(gram)
33,4415
31,8652
Kadar Abu =
(
=
II.
Kadar Abu =
,
,
%
Kadar
Abu
Larut
Air
3,68%
3,34%
)
,
,
,
,
× 100%
× 100% = 3,34%
= 3,51%
\
80
3,51% ±0,24
× 100%
)
,
Rata – rata ±
SD
× 100% = 3,68%
,
(
=
Rata – Rata =
W
(Krus +
kertas
saring)
(gram)
33,3677
31,7983
LAMPIRAN L
HASIL PERHITUNGAN KADAR ABU TIDAK LARUT ASAM
No
W
(krus
kosong)
(gram)
Bobot
abu
awal
(gram)
1.
2.
32,1937
31,7524
2,0097
2,0087
I.
W
(Krus
+
bahan)
(gram)
32,2276
31,7941
Kadar Abu =
32,2013
31,7629
(
)
=
II.
W (Krus
+ kertas
saring)
(gram)
,
,
(
)
Rata – Rata =
,
,
,
Rata – rata ±
SD
1,43% ±0,170
× 100%
× 100% = 1,31%
,
Kadar Abu =
=
%
Kadar
Abu
Larut
Air
1,31%
1,55%
,
,
= 1,43%
81
× 100%
× 100% = 1,55%
LAMPIRAN M
HASIL PERHITUNGAN KADAR AIR EKSTRAK
No. W (cawan W (Zat) W (cawan +% Kadar Rata – rata± SD
kosong)
(gram)
simplisia)
(gram)
Air
(gram)
1.
83,5648
5,0054
88,1658
8,01%
2.
88,1440
5,0012
92,7140
8,62%
3.
79,0626
5,0051
83,6179
8,98%
I.
× 100%
Kadar Air =
,
=
II.
,
× 100% = 8,01%
,
× 100%
Kadar Abu =
,
=
III.
8,54%± 0,490
,
× 100% = 8,62%
,
× 100%
Kadar Abu
,
=
Rata – Rata =
,
,
,
,
,
= 8,54%
82
× 100% = 8,98%
LAMPIRAN N
PERHITUNGAN PENIMBANGAN EKSTRAK
Berat Badan Sebelum Perlakuan (gram)
Kelompok Kelompok Perlakuan 1 elompok Perlakuan 1 Kelompok Perlakuan 1
Kontrol
(Dosis 550
(Dosis 1750
(Dosis 5000 mg/kgBB)
mg/kgBB)
mg/kgBB)
26
21
33
28
29
29
28
27
28
26
29
36
17
32
29
33
Berikut adalah perhitungan penimbangan ekstrak untuk mencit jantan
galur Swiss :
Vp =
(
)
(
/
)
,
Keterangan :
Vp : Faktor Pengenceran
D
: Dosis
C
: Konsentrasi,
berdasarkan rumus diatas maka dapat dihitung :
Dosis 550 mg/kgBB :
Vp(2,5ml)=
C=
× 550 : C = 59,4 mg = 0,0594 gram
0,0594 gram
2 ml
= 0,0297 gram/ ml X 5 ml = 0,1485 gram/ 5ml
Dosis 1750 mg/kgBB :
Vp(2,5ml)=
C=
× 1750 : C = 208, 25mg = 0,20825 gram
0,20825 gram
2 ml
= 0,1041 gram/ ml X 5 ml = 0,5206 gram/ 5ml
Dosis 5000 mg/kgBB :
Vp(2,5ml)=
C=
× 5000 : C = 620 mg = 0,62 gram
0,62 gram
2 ml
= 0,31 gram/ ml X 5 ml = 1,55 gram/ 5ml
83
LAMPIRAN O
PENIMBANGAN ORGAN (gram)
Kode
BB
awal
BB akhir
Bobot Organ (gram)
Jantung
Ginjal
Paru
Testis
Limfa
Hati
Vas. Differens
84
Kontrol 1
16
28
0,16
0,48
0,34
0,28
0,42
2,16
0,22
Kontrol 2
17
31
0,14
0,43
0,23
0,23
0,19
1,48
0,22
Kontrol 3
17
31
0,16
0,61
0,29
0,31
0,26
1,99
0,26
Kontrol 4
15
21
0,14
0,23
0,27
0,28
0,24
1,82
0,26
Dosis 550 mg/kgBB
16
25
0,09
0,34
0,19
0,24
0,25
1,31
0,15
Dosis 550 mg/kgBB
17
31
0,14
0,45
0,25
0,28
0,31
2,07
0,38
Dosis 550 mg/kgBB
16
32
0,16
0,33
0,35
0,34
0,13
1,51
0,27
Dosis 550 mg/kgBB
19
34
0,16
0,48
0,24
0,24
0,15
1,44
0,18
Dosis 1750 mg/kgBB
20
35
0,15
0,48
0,22
0,34
0,24
2,05
0,37
Dosis 1750 mg/kgBB
17
30
0,14
0,50
0,24
0,26
0,24
0,94
0,16
Dosis 1750 mg/kgBB
18
33
0,13
0,51
0,25
0,25
0,48
1,75
0,21
Dosis 1750 mg/kgBB
18
32
0,11
0,38
0,31
0,23
0,13
1,39
0,27
Dosis 5000 mg/kgBB
17
29
0,16
0,49
0,26
0,29
0,22
1,84
0,36
Dosis 5000 mg/kgBB
17
29
0,12
0,44
0,21
0,23
0,22
1,75
0,19
Dosis 5000 mg/kgBB
20
34
0,14
0,46
0,32
0,23
0,26
1,76
0,31
Dosis 5000 mg/kgBB
19
34
0,13
0,48
0,24
0,27
0,16
1,49
0,35
85
LAMPIRAN P
PERHITUNGAN INDEKS ORGAN (%)
Jantung
Ginjal
0,16
ontrol 1
Paru
0,48
× 100
28
= 0,57
0,34
× 100
28
= 1,21
28
× 100
= 1,71
Testis
Limpa
0,28
0,42
× 100
28
= 1,00
Hati
Vas Differens
2,16
× 100
28
= 1,50
0,22
× 100
28
= 0,79
28
× 100
= 7,71
86
ontrol 2
45
39
74
74
61
77
71
ontrol 3
52
97
94
00
84
42
84
ontrol 4
67
95
29
33
14
67
24
0,36
1,36
0,76
0,96
1,00
5,24
0,60
Dosis 550 mg/kgBB
0,45
1,45
0,81
0,90
1,00
6,67
1,22
Dosis 550 mg/kgBB
0,50
1,22
1,30
1,26
0,48
5,59
1,00
Dosis 550 mg/kgBB
0,47
1,41
0,71
0,70
0,44
4,24
0,53
0,43
1,37
0,63
0,97
0,69
5,86
1,06
Dosis 1750 mg/kgBB
0,47
1,67
0,80
0,87
0,80
3,13
0,53
Dosis 1750 mg/kgBB
0,39
1,55
0,76
0,76
1,45
5,30
0,64
osis 550 mg/kgBB
osis 1750 mg/kgBB
0,34
Dosis 1750 mg/kgBB
1,19
0,97
0,72
0,41
4,34
0,84
55
69
90
00
76
34
24
Dosis 5000 mg/kgBB
41
52
72
79
76
03
66
Dosis 5000 mg/kgBB
41
35
94
68
76
18
91
Dosis 5000 mg/kgBB
38
41
71
79
47
38
03
osis 5000 mg/kgBB
87
LAMPIRAN Q
HASIL PENGAMATAN KELOMPOK KONTROL
Keterangan :
+
: Ada Aktivitas
: Tidak Ada Aktivitas
Kj
: Kuning Jernih
P
: Padat
88
89
LAMPIRAN R
HASIL PENGAMATAN KELOMPOK PERLAKUAN DOSIS 550 mg/kgBB
90
91
LAMPIRAN S
HASIL PENGAMATAN KELOMPOK PERLAKUAN DOSIS 1750 mg/kgBB
92
93
LAMPIRAN T
HASIL PENGAMATAN KELOMPOK PERLAKUAN DOSIS 5000 mg/kgBB
94
95
LAMPIRAN U
ORGAN KELOMPOK KONTROL DAN KELOMPOK PERLAKUAN
Kontrol 1
Kontrol 2
96
Kontrol 3
Kontrol 4
97
Dosis 550 mg/kgBB (5)
Dosis 550 mg/kgBB (6)
98
Dosis 550 mg/kgBB (7)
Dosis 550 mg/kgBB (8)
99
Dosis 1750 mg/kgBB (9)
Dosis 1750 mg/kgBB (10)
100
Dosis 1750 mg/kgBB (11)
Dosis 1750 mg/kgBB (12)
101
Dosis 5000 mg/kgBB (13)
Dosis 5000 mg/kgBB (14)
102
Dosis 5000 mg/kgBB (15)
Dosis 5000 mg/kgBB (16)
103
LAMPIRAN V
LAMBUNG KELOMPOK KONTROL DAN KELOMPOK
PERLAKUAN
Kontrol
Perlakuan Dosis 550 mg/kgBB
Perlakuan Dosis 1750 mg/kgBB
Perlakuan Dosis 5000 mg/kgBB
104
LAMPIRAN W
PERHITUNGAN JUMLAH JENGUKAN DENGAN ONE WAY ANOVA
Test of Homogeneity of Variances
Jumlah_Jengukan
Levene Statistic
df1
df2
1.556
3
Sig.
12
.251
ANOVA
Jumlah_Jengukan
Sum of
Squares
Between Groups
Within Groups
Total
Df
8.188
50.250
58.438
Mean Square
3
12
15
F
Sig.
2.729 .652 .597
4.188
Test of Homogeneity of Variances
Jumlah_jengukan
Levene Statistic
df1
7.643
df2
Sig.
3
12
.004
ANOVA
Jumlah_jengukan
Sum of
Squares
Between Groups
Within Groups
Total
10.188
37.750
47.938
Df
3
12
15
Mean Square
3.396
3.146
Test of Homogeneity of Variances
102
F
1.079
Sig.
.395
Jumlah_Jengukan
Levene Statistic
df1
df2
.770
3
Sig.
12
.533
Mean
Square
F
ANOVA
Jumlah_Jengukan
Sum of Squares
Between Groups
Within Groups
Total
Df
15.500
23.500
39.000
3
12
15
Sig.
5.167 2.638 .097
1.958
Test of Homogeneity of Variances
Jumlah_Jengukan
Levene Statistic
.404
df1
df2
3
Sig.
12
.753
ANOVA
Jumlah_Jengukan
Sum of Squares
Mean
Square
Df
Between Groups
29.500
3
Within Groups
Total
11.500
41.000
12
15
103
F
9.833 10.26
1
.958
Sig.
.001
Post Hoc Tests
Multiple Comparisons
Jumlah_Jengukan
Tukey HSD
(I) Parameter
(J) Parameter
Kontrol
Dosis 550 mg/kgBB
Mean
Difference (I-J)
Dosis 1750 mg/kgBB
Dosis 5000 mg/kgBB
Dosis 550
mg/kgBB
104
Dosis 1750
mg/kgBB
Dosis 5000
mg/kgBB
Kontrol
95% Confidence Interval
Std. Error
Sig.
Lower Bound
Upper Bound
-.750
.692
.706
-2.81
*
.692
.016
-4.56
-.44
1.250
.692
.317
-.81
3.31
2.81
-2.500
1.31
.750
.692
.706
-1.31
Dosis 1750 mg/kgBB
-1.750
.692
.105
-3.81
.31
Dosis 5000 mg/kgBB
2.000
.692
.057
-.06
4.06
2.500*
.692
.016
.44
4.56
Dosis 550 mg/kgBB
1.750
.692
.105
-.31
3.81
Dosis 5000 mg/kgBB
3.750*
.692
.001
1.69
5.81
Kontrol
-1.250
.692
.317
-3.31
.81
Dosis 550 mg/kgBB
-2.000
.692
.057
-4.06
.06
Dosis 1750 mg/kgBB
-3.750*
.692
.001
-5.81
-1.69
Kontrol
*. The mean difference is significant at the 0.05 level.
Homogeneous Subsets
Jumlah_Jengukan
Tukey HSDa
Subset for alpha = 0.05
Parameter
N
1
2
Dosis 5000 mg/kgBB
4
1.50
Kontrol
4
2.75
Dosis 550 mg/kgBB
4
3.50
Dosis 1750 mg/kgBB
4
Sig.
.057
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 4,000.
3.50
5.25
.105
Test of Homogeneity of Variances
Jumlah_Jengukan
Levene Statistic
df1
1.493
df2
Sig.
3
12
.266
ANOVA
Jumlah_Jengukan
Sum of
Squares
Between Groups
Within Groups
Total
23.500
36.500
60.000
Df
Mean Square
3
12
15
105
F
7.833 2.575
3.042
Sig.
.103
Test of Homogeneity of Variances
Jumlah_Jengukan
Levene Statistic
df1
df2
1.338
Sig.
3
12
.308
ANOVA
Jumlah_Jengukan
Sum of
Squares
Between Groups
Within Groups
Total
Mean
Square
df
32.188
57.250
89.438
3
12
15
F
10.729
4.771
Sig.
2.249
.135
Test of Homogeneity of Variances
Jumlah_Jengukan
Levene Statistic
df1
2.267
df2
3
Sig.
12
.133
ANOVA
Jumlah_Jengukan
Sum of
Squares
Between Groups
Within Groups
Total
13.688
72.750
86.438
Mean
Square
Df
3
12
15
106
4.563
6.063
F
.753
Sig.
.542
Test of Homogeneity of Variances
Jumlah_Jengukan
Levene Statistic
df1
1.811
df2
3
Sig.
12
.199
ANOVA
Jumlah_Jengukan
Sum of
Squares
Between Groups
Within Groups
Total
7.188
42.250
49.438
Mean
Square
Df
3
12
15
107
2.396
3.521
F
.680
Sig.
.581
LAMPIRAN X
PERHITUNGAN WAKTU MENGGELANTUNG DENGAN ONE WAY
ANOVA
Test of Homogeneity of Variances
Waktu_Menggelantung
Levene Statistic
df1
df2
5.697
Sig.
3
12
.012
ANOVA
Waktu_Menggelantung
Sum of
Squares
Between
Groups
Within Groups
Total
Mean
Square
df
72.610
3
24.203
258.031
330.640
12
15
21.503
F
Sig.
1.126
.378
Test of Homogeneity of Variances
Waktu_Menggelantung
Levene Statistic
df1
34.762
df2
Sig.
3
12
.000
ANOVA
Waktu_Menggelantung
Sum of
Squares
Between Groups
Within Groups
Total
241.470
625.710
867.180
df
Mean Square
3
12
15
108
80.490
52.142
F
1.544
Sig.
.254
Test of Homogeneity of Variances
Waktu_menggelantung
Levene Statistic
df1
df2
.492
Sig.
3
12
.694
ANOVA
Waktu_menggelantung
Sum of
Squares
Between Groups
Within Groups
Total
Mean
Square
Df
82.228
820.385
902.613
3
12
15
F
27.409
68.365
Sig.
.401
.755
Test of Homogeneity of Variances
Waktu_Menggelantung
Levene Statistic
df1
df2
.617
3
Sig.
12
.617
Mean
Square
F
ANOVA
Waktu_Menggelantung
Sum of
Squares
Between Groups
Within Groups
Total
8.383
489.224
497.607
Df
3 2.794
12 40.769
15
109
.069
Sig.
.976
Test of Homogeneity of Variances
Waktu_Menggelantung
Levene Statistic
df1
6.000
df2
3
Sig.
12
.010
ANOVA
Waktu_Menggelantung
Sum of
Squares
Between
Groups
Within Groups
Total
Mean
Square
df
F
30.583
3
10.194
183.493
214.076
12
15
15.291
Sig.
.667
.588
Test of Homogeneity of Variances
Waktu_Menggelantung
Levene Statistic
df1
3.882
df2
3
Sig.
12
.038
ANOVA
Waktu_Menggelantung
Sum of
Squares
Between Groups
Within Groups
Total
57.756
535.608
593.364
Mean
Square
df
3
12
15
F
19.252
44.634
Test of Homogeneity of Variances
Waktu_Menggelantung
Levene Statistic
3.747
df1
df2
3
Sig.
12
110
.041
.431
Sig.
.734
ANOVA
Waktu_Menggelantung
Sum of
Squares
Between Groups
Df
Mean Square
60.182
3
20.061
Within Groups
578.158
12
48.180
Total
638.339
15
F
Sig.
.416
.744
Test of Homogeneity of Variances
Waktu_Menggelantung
Levene Statistic
df1
.711
df2
Sig.
3
12
.564
ANOVA
Waktu_Menggelantung
Sum of
Squares
Between
Groups
Within Groups
Total
Df
Mean Square
208.195
3
69.398
1097.507
1305.701
12
15
91.459
111
F
.759
Sig.
.538
LAMPIRAN Y
PERHITUNGAN INDEKS ORGAN DENGAN ONE WAY ANOVA
Test of Homogeneity of Variances
Organ_Ginjal
Levene Statistic
df1
.573
df2
3
Sig.
12
.644
ANOVA
Organ_Ginjal
Sum of
Squares
Between Groups
Within Groups
Total
Mean
Square
Df
.408
.525
.933
3
12
15
F
.136
.044
3.109
Sig.
.067
Test of Homogeneity of Variances
Organ_Hati
Levene Statistic
df1
.432
df2
3
Sig.
12
.734
ANOVA
Organ_Hati
Sum of
Squares
Between Groups
Within Groups
Total
9.453
17.812
27.265
Mean
Square
Df
3
12
15
112
3.151
1.484
F
2.123
Sig.
.151
Test of Homogeneity of Variances
Organ_Jantung
Levene Statistic
df1
df2
.321
3
Sig.
12
.810
ANOVA
Organ_Jantung
Sum of
Squares
Between Groups
Within Groups
Total
df
.046
.062
.108
Mean
Square
3
12
15
F
.015 2.990
.005
Sig.
.073
Test of Homogeneity of Variances
Organ_Limpa
Levene Statistic
df1
1.367
df2
3
Sig.
12
.300
ANOVA
Organ_Limpa
Sum of
Squares
Between Groups
Within Groups
Total
Mean
Square
df
.281
1.421
1.702
3
12
15
F
.094
.118
.792
Test of Homogeneity of Variances
Organ_Paru
Levene Statistic
1.711
df1
df2
3
Sig.
12
113
.218
Sig.
.521
ANOVA
Organ_Paru
Sum of
Squares
Between Groups
Within Groups
Total
Mean
Square
df
.163
.378
.541
3
12
15
F
.054
.032
1.724
Sig.
.215
Test of Homogeneity of Variances
Organ_Testis
Levene Statistic
df1
df2
.330
3
Sig.
12
.804
ANOVA
Organ_Testis
Sum of
Squares
Between Groups
Within Groups
Total
Mean
Square
df
.121
.301
.422
3
12
15
F
.040
.025
Test of Homogeneity of Variances
Organ_vasDifferen
Levene Statistic
.185
df1
df2
3
Sig.
12
114
.904
1.611
Sig.
.239
ANOVA
Organ_vasDifferen
Sum of
Squares
Between Groups
Within Groups
Total
Mean
Square
df
.094
.797
.892
3
12
15
115
.031
.066
F
.473
Sig.
.707