Analisis Keragaman Genetik Klon Kelapa Sawit (Elaeis guineensis Jacq.) Plasma Nutfah PT. Socfindo Menggunakan Marka RAPD (Random Amplified Polymorphic DNA)
ABSTRACT
HERMANYANTO LAIA, 2017 : Genetic Diversity of Oil Palm
(Elaeis guinnensis Jacq.) Clones Germaplasm of PT. Socfindo Based on RAPD
(Random Amplified Polymorphic DNA). Supervised by LOLLIE AGUSTINA P.
PUTRI andKHAIRUNISA LUBIS.
The objective of this research was to analyze genetic diversity of Oil Palm Clones
based on RAPD used marker OPD-13, OPD-20 and OPM-01. This research was
conduted at Plant Tissue Culture Laboratory, Faculty of Agriculture, University
of Sumatera Utara, Medan, from March 2017 until June 2017. Genetic materials
were 30 DNA stock of oil palm BTC-60 clone from PT. Socfindo. A total 13 bands
were scored, with the range of band size was 405 bp – 2268 bp and average of
polymorphism was 39,29%. Genetic distance measurement and dendrogram
formation were analyzed using DARwin 6.0 software. Based on the analysis, 30
clones of oil palm grouped into 3 clusters with a total of molecular diversity was
65,89%. The range of dissimilarity coefficient was 0-0,24.
Keywords
: clone, genetic diversity, oil palm, RAPD
i
Universitas Sumatera Utara
ABSTRAK
HERMANYANTO LAIA, 2017 : Analisis Keragaman Genetik Klon Kelapa
Sawit (Elaeis guinnensis Jacq.) Plasma Nutfah PT. Socfindo Menggunakan Marka
RAPD (Random Amplified Polymorphic DNA). Dibimbing oleh LOLLIE
AGUSTINA P. PUTRI dan KHAIRUNISA LUBIS.
Penelitian ini bertujuan mengetahui keragaman genetik klon kelapa sawit
plasma nutfah PT. Socfindo berdasarkan marka Random Amplified Polymorphic
DNA (RAPD) menggunakan primer OPD-13, OPD-20 dan OPM-01. Penelitian ini
dilaksanakan di Laboratorium Kultur Jaringan Tanaman, Fakultas Pertanian
Universitas Sumatera Utara, Medan, dimulai dari bulan Maret 2017 hingga Juni
2017. Materi genetik yang dianalisis merupakan 30 stok DNA kelapa sawit klon
BTC-60 dari PT. Socfindo. Didapatkan 13 pita dengan ukuran berkisar antara
369 bp – 2586 bp dan rataan persentase polimorfisme 39,29%. Perhitungan jarak
dan pembentukan dendrogram dilakukan menggunakan software DARwin 6.0.
Hasil analisis tersebut menunjukan bahwa 30 klon tersebut terbagi atas 3
kelompok utama dengan total keragaman molekuler 65,89%. Koefisien jarak
genetik berkisar antara 0-0,24.
Kata Kunci
: kelapa sawit, keragaman genetik, klon, RAPD
ii
Universitas Sumatera Utara
HERMANYANTO LAIA, 2017 : Genetic Diversity of Oil Palm
(Elaeis guinnensis Jacq.) Clones Germaplasm of PT. Socfindo Based on RAPD
(Random Amplified Polymorphic DNA). Supervised by LOLLIE AGUSTINA P.
PUTRI andKHAIRUNISA LUBIS.
The objective of this research was to analyze genetic diversity of Oil Palm Clones
based on RAPD used marker OPD-13, OPD-20 and OPM-01. This research was
conduted at Plant Tissue Culture Laboratory, Faculty of Agriculture, University
of Sumatera Utara, Medan, from March 2017 until June 2017. Genetic materials
were 30 DNA stock of oil palm BTC-60 clone from PT. Socfindo. A total 13 bands
were scored, with the range of band size was 405 bp – 2268 bp and average of
polymorphism was 39,29%. Genetic distance measurement and dendrogram
formation were analyzed using DARwin 6.0 software. Based on the analysis, 30
clones of oil palm grouped into 3 clusters with a total of molecular diversity was
65,89%. The range of dissimilarity coefficient was 0-0,24.
Keywords
: clone, genetic diversity, oil palm, RAPD
i
Universitas Sumatera Utara
ABSTRAK
HERMANYANTO LAIA, 2017 : Analisis Keragaman Genetik Klon Kelapa
Sawit (Elaeis guinnensis Jacq.) Plasma Nutfah PT. Socfindo Menggunakan Marka
RAPD (Random Amplified Polymorphic DNA). Dibimbing oleh LOLLIE
AGUSTINA P. PUTRI dan KHAIRUNISA LUBIS.
Penelitian ini bertujuan mengetahui keragaman genetik klon kelapa sawit
plasma nutfah PT. Socfindo berdasarkan marka Random Amplified Polymorphic
DNA (RAPD) menggunakan primer OPD-13, OPD-20 dan OPM-01. Penelitian ini
dilaksanakan di Laboratorium Kultur Jaringan Tanaman, Fakultas Pertanian
Universitas Sumatera Utara, Medan, dimulai dari bulan Maret 2017 hingga Juni
2017. Materi genetik yang dianalisis merupakan 30 stok DNA kelapa sawit klon
BTC-60 dari PT. Socfindo. Didapatkan 13 pita dengan ukuran berkisar antara
369 bp – 2586 bp dan rataan persentase polimorfisme 39,29%. Perhitungan jarak
dan pembentukan dendrogram dilakukan menggunakan software DARwin 6.0.
Hasil analisis tersebut menunjukan bahwa 30 klon tersebut terbagi atas 3
kelompok utama dengan total keragaman molekuler 65,89%. Koefisien jarak
genetik berkisar antara 0-0,24.
Kata Kunci
: kelapa sawit, keragaman genetik, klon, RAPD
ii
Universitas Sumatera Utara