Uji Toksisitas Akut Ekstrak Etanol Buah Adas Manis (Foeniculum vulgare Mill.) Pada Mencit

Lampiran 1. Surat hasil identifikasi sampel

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Lampiran 2. Gambar tumbuhan dan buah adas manis (Foeniculum vulgare)

Tumbuhan adas manis

Buah adas manis

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Lampiran 3. Rekomendasi persetujuan etik penelitian kesehatan

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Lampiran 4. Bagan kerja penelitian
500g buah adas manis
Dibersihkan
Dimaserasi dengan pelarut etanol 80%
Maserat

Diuapkan dengan alat rotary evaporator pada
temperatur  400C
Ekstrak kental etanol
buah adas manis
Dikeringkan dengan freeze dryer
pada suhu -40oC
Ekstrak kering buah adas
manis

Skrining fitokimia untuk
pemeriksaan :
a. Alkaloida
b. Flavanoida
c. Glikosida
d. Antrakinon glikosida
e. Saponin
f. Tanin
g. Steroida / triterpenoida

Uji toksisitas akut

Hasil

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Lampiran 5. Bagan penelitian uji toksisitas ekstrak etanol buah adas manis
40 ekor mencit jantan

Randomisasi

Aklimatisasi (7 hari)

Kontrol

+
CMC
Na
1%

P1

P2

P3

P4

P5

P6

P7

+
EEBA
M
5
mg/kg
bb

+
EEBAM
50
mg/kg
bb

+
EEBAM
300
mg/kg
bb

+
EEBAM
1000
mg/kg
bb

+
EEBAM

2000
mg/kg
bb

+
EEBAM
4000
mg/kg
bb

+
EEBAM
8000
mg/kg
bb

Gejala klinis, konsumsi makanan dan minuman, berat badan, kematian,
makropatologi organ hewan coba (14 hari)

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Lampiran 6. Contoh perhitungan dosis
Berat badan mencit 25 kg dengan dosis EEBAM 1000 mg/kg bb
Dosis = 1000 mg/kg bb
= (25 : 1000 kg) x 1000 mg/kg
= 25 mg

Konsentrasi ekstrak yang dibuat 8 % = 8 g/ 100 ml
= 8000 mg/100 ml
= 80 mg/ml

Jumlah obat yang disuntik

= 25 mg : 80 mg/ml
= 0,31 ml

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Lampiran 7. Hasil pengukuran berat badan
1.

Berat badan pada minggu pertama
Berat badan hari ke- (g)

Perlakuan
kontrol I

0
25,78

3
28,08

7
28,02

kontrol II

27,09

29,48

28,37

kontrol III

29,98

29,89

29,17

kontrol IV

30,16

30,79

29,93

kontrol V

30,84

30,81

30,66

Dosis 5 mg/kg bb

27,86

28,05

28,28

Dosis 5 mg/kg bb

28,19

28,16

29,25

Dosis 5 mg/kg bb

28,55

29,48

30,56

Dosis 5 mg/kg bb

29,68

29,99

31,39

Dosis 5 mg/kg bb

30,52

31,57

31,77

Dosis 50 mg/kg bb

27,25

27,55

28,78

Dosis 50 mg/kg bb

27,68

28,87

29,69

Dosis 50 mg/kg bb

28,46

29,61

29,84

Dosis 50 mg/kg bb

30,28

29,67

30,56

Dosis 50 mg/kg bb

31,98

31,55

31,03

Dosis 300 mg/kg bb

30,72

32,14

33,18

Dosis 300 mg/kg bb

28,69

29,09

29,24

Dosis 300 mg/kg bb

29,55

29,73

29,83

Dosis 300 mg/kg bb

29,5

31,01

31,13

Dosis 300 mg/kg bb
Dosis 1000 mg/kg bb

29,96
31,02

30,17
31,19

30,59
33,11

Dosis 1000 mg/kg bb

28,97

28,63

28,77

Dosis 1000 mg/kg bb

29,28

31,27

28,15

Dosis 1000 mg/kg bb

25,94

27,42

29,55

Dosis 1000 mg/kg bb

29,03

30,43

29,03

Dosis 2000 mg/kg bb

30,97

32,84

31.69

Dosis 2000 mg/kg bb

28,93

29,01

29,29

Dosis 2000 mg/kg bb

29,03

30,26

31,28

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Lampiran 7. (lanjutan)
1.

Berat badan pada minggu pertama
Perlakuan

Berat badan hari ke- (g)
0

3

7

Dosis 2000 mg/kg bb

29,05

29,65

30,41

Dosis 2000 mg/kg bb

29,09

30,95

30,59

Dosis 4000 mg/kg bb

32,65

31,33

33,98

Dosis 4000 mg/kg bb

28,95

29,45

28,65

Dosis 4000 mg/kg bb

31,09

31,66

31,93

Dosis 4000 mg/kg bb

31,02

30,16

31,13

Dosis 4000 mg/kg bb

30,96

30,86

32,39

Dosis 8000 mg/kg bb

31,26

31,93

33,19

Dosis 8000 mg/kg bb

29,21

30,68

29,26

Dosis 8000 mg/kg bb

30,47

30,54

31,38

Dosis 8000 mg/kg bb

30,81

30,89

31,37

Dosis 8000 mg/kg bb

31,15

31,88

31,53

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Lampiran 7. (lanjutan)
2.

Berat badan pada minggu kedua
Berat badan hari ke- (g)

Perlakuan
kontrol I

0
25,78

10
28,89

14
29,07

kontrol II

27,09

29,02

29,13

kontrol III

29,98

29,16

29,98

kontrol IV

30,16

30,38

31,13

kontrol V

30,84

31,45

31,24

Dosis 5 mg/kg bb

27,86

29,17

29,97

Dosis 5 mg/kg bb

28,19

29,43

30,66

Dosis 5 mg/kg bb

28,55

30,98

30,74

Dosis 5 mg/kg bb

29,68

31,08

32,64

Dosis 5 mg/kg bb

30,52

32,14

32,69

Dosis 50 mg/kg bb

27,25

29,29

29,86

Dosis 50 mg/kg bb

27,68

29,42

30,19

Dosis 50 mg/kg bb

28,46

29,68

30,99

Dosis 50 mg/kg bb

30,28

31,65

31,18

Dosis 50 mg/kg bb

31,98

31,66

31,68

Dosis 300 mg/kg bb

30,72

33,16

34,16

Dosis 300 mg/kg bb

28,69

29,33

30,93

Dosis 300 mg/kg bb

29,55

31,09

32,21

Dosis 300 mg/kg bb

29,5

31,98

33,14

Dosis 300 mg/kg bb
Dosis 1000 mg/kg bb

29,96
31,02

31,58
32,24

32,64
33,72

Dosis 1000 mg/kg bb

28,97

29,19

30,49

Dosis 1000 mg/kg bb

29,28

30,99

32,37

Dosis 1000 mg/kg bb

25,94

30,91

32,43

Dosis 1000 mg/kg bb

29,03

30,88

32,54

Dosis 2000 mg/kg bb

30,97

33,25

33,48

Dosis 2000 mg/kg bb

28,93

29,86

30,44

Dosis 2000 mg/kg bb

29,03

30,63

31,85

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Lampiran 7. (lanjutan)
2.

Berat badan pada minggu kedua
Perlakuan

Berat badan hari ke- (g)
0

10

14

Dosis 2000 mg/kg bb

29,05

30,63

31,61

Dosis 2000 mg/kg bb

29,09

31,75

32,11

Dosis 4000 mg/kg bb

32,65

34,12

34,17

Dosis 4000 mg/kg bb

28,95

30,91

31,75

Dosis 4000 mg/kg bb

31,09

32,06

33,15

Dosis 4000 mg/kg bb

31,02

31,82

32,98

Dosis 4000 mg/kg bb

30,96

32,66

34,68

Dosis 8000 mg/kg bb

31,26

33,18

33,68

Dosis 8000 mg/kg bb

29,21

32,19

32,78

Dosis 8000 mg/kg bb

30,47

31,54

31,82

Dosis 8000 mg/kg bb

30,81

32,19

32,61

Dosis 8000 mg/kg bb

31,15

32,36

32,55

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Lampiran 8. Perhitungan karakterisasi simplisia buah adas manis
a. Penetapan kadar air

% kadar air 

volume air
 100%
berat sampel

No.

Berat sampel
(g)

Volume air
awal

Volume air akhir

Volume air
sebenarnya

1

5,0000

1,4000

1,7500

0,3500

2

5,1550

2,0000

2,4500

0,4500

3

5,0880

2,4500

2,9000

0,4500

Volume air

= 0,3500 ml

1. Berat sampel

= 5,0000 g

Kadar air

2. Berat sampel

=

= 5,1550 g
Volume air
Kadar air

3. Berat sampel

=

= 0,4500 ml

0,4500
 100%  8,7294%
5,1550

= 5,0880 g
Volume air
Kadar air

Rata-rata kadar air:

0,3500
 100%  7,0000%
5,0000

=

= 0,4500 ml

0,4500
 100%  8,1912%
5,0880

7,0000  8,7294  8,1912
 7,9735%
3

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Lampiran 8. (Lanjutan)
b. Penetapan kadar sari larut etanol

% kadar sari larut etanol 

berat sari larut etanol 100

 100%
berat sampel
20

No.

Berat
sampel (g)

Berat cawan

Berat cawan + sari larut
etanol

Berat sari larut
etanol

1

5,0180

29,6493

29,8053

0,1560

2

5,0270

25,0725

25,2349

0,1624

3

5,0195

37,9111

38,0628

0,1417

1. Berat sampel

= 5,0180 g

Berat sari = 0,1560 g
Kadar sari

=

0,1560 100

 100%  15,5440%
5,0180 20

2. Berat sampel

= 5,0270 g

Berast sari

= 0,1624 g

Kadar sari

=

0,1624 100

 100%  16,1528%
5,0270 20

3. Berat sampel

= 5,0195 g

Berast sari

= 0,1417 g

Kadar sari

=

0,1417 100

 100%  15,1111%
5,0195 20

Rata-rata kadar sari larut etanol:

15,5440  16,1528  15,1111
 15,6026%b / v
3

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Lampiran 8. (Lanjutan)
c. Penetapan kadar sari larut air

% kadar sari larut air 

berat sari larut air 100

 100%
berat sampel
20

No.

Berat cawan

Berat sampel (g)

Berat cawan + sari
larut air

Berat sari
larut air

1

49,7330

5,0235

50,0341

0,3011

2

49,4780

5,0310

49,7325

0,2545

3

47,7360

5,0229

48,0537

0,3177

1. Berat sampel

= 5,0235 g

Berast sari

= 0,3011 g

Kadar sari

=

0,3011 100

 100%  29,9691%b / v
5,0235 20

2. Berat sampel

= 5,0310 g

Berast sari

= 0,2545 g

Kadar sari

=

0,2545 100

 100%  25,2932%b / v
5,0310 20

3. Berat sampel

= 5,0229 g

Berast sari

= 0,3177 g

Kadar sari

=

0,3177 100

 100%  31,6252%b / v
5,0229 20

Rata-rata kadar sari larut air:

29,9691  25,2932  31,6252
 28,9625%b / v
3

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Lampiran 8. (Lanjutan)
d. Penetapan kadar abu total

% kadar abu total 

berat abu
 100%
berat sampel

NO.

Berat krus

Berat sampel (g)

Berat krus + abu

Berat abu

1

53,6220

2,0700

53,7790

0,1570

2

50,1090

2,1530

50,2820

0,1730

3

61,6460

2,0520

61,7950

0,1490

1. Berat sampel

= 2,0700 g

Berat abu

= 0,1570 g

Kadar abu

=

0,1570
 100%  7,5845%
2,0700

2. Berat sampel

= 2,1530 g

Berat abu

= 0,1730 g

Kadar abu

=

0,1730
 100%  8,0350%
2,1530

3. Berat sampel

= 2,0520 g

Berat abu

= 0,1490 g

Kadar abu

=

0,1490
 100%  7,2612%
2,0520

Rata-rata kadar abu total:

7,5845  8,0350  7,2612
 7,6269%
3

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Lampiran 8. (Lanjutan)
e. Penetapan kadar abu tidak larut asam
% kadar abu tidak larut asam 

berat abu tidak larut asam
 100%
berat sampel

No.

Berat sampel (g)

Berat krus

Berat krus + abu
tidak larut asam

Berat abu tidak
larut asam

1

2,0700

53,6220

53,6520

0,0300

2

2,0520

50,1090

50,1300

0,0210

3

2,0520

61,6460

61,6690

0,0230

1. Berat sampel

= 2, 0520 g

Berat abu

= 0,0300 g

Kadar abu

=

0,0300
 100%  1,4493%
2,0520

2. Berat sampel

= 2,0520 g

Berat abu

= 0,0210 g

Kadar abu

=

0,0210
 100%  1,0234%
2,0520

3. Berat sampel

= 2,0520 g

Berat abu

= 0,0230 g

Kadar abu

=

0,0230
 100%  1,1209%
2,0520

Rata-rata kadar abu tidak larut asam =

1,4493  1,0234  1,1209
 1,1979%
3

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Lampiran 9. Perhitungan karakterisasi ekstrak etanol buah adas manis
a. Penetapan kadar air
kadar air 

volume air
 100%
berat sampel

No.

Beratsampel (g)

Volume air 1

Volume air 2

Volume air

1

5,0253

2,1

2,5

0,4000

2

5,1725

2,5

2,9

0,4000

3

5,0623

2,9

3,4

0,4000

1. Berat sampel

= 5,0253 g
Volume air
Kadar air

2. Berat sampel

=

Kadar air

=

= 0,4000 ml

0,4000
 100%  7,7332%
5,1725

= 5,0623 g
Volume air
Kadar air

Rata-rata kadar air:

0,4000
 100%  7,9597%
5,0253

= 5,1725 g
Volume air

3. Berat sampel

= 0,4000 ml

=

= 0,4000 ml

0,4000
 100%  7,9015%
5,0623

7,9597  7,7332  7,9015
 7,8648%
3

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Lampiran 9. (Lanjutan)
b. Penetapan kadar abu total
kadar abu total 

berat abu
100%
berat sampel

No

Berat sampel
(g)

Berat krus

Berat krus + abu

Berat abu total

1

2,0938

63,5387

63,5611

0,0224

2

2,0082

58,2095

58,2241

0,0146

3

2,0179

57,2289

57,2466

0,0177

1. Berat sampel

= 2,0938 g

Berat abu

= 0,0224 g

Kadar abu

=

0,0224
 100%  1,0698%
2,0938

2. Berat sampel

= 2,0082 g

Berat abu

= 0,0146 g

Kadar abu

=

0,0146
 100%  0,7270%
2,0082

3. Berat sampel

= 2,0179 g

Berat abu

= 0,0177 g

Kadar abu

Rata-rata kadar abu total:

=

0,0177
 100%  0,8771%
2,0179

1,0698  0,7270  0,8771
 0,8913%
3

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Lampiran 9. (Lanjutan)
c. Penetapan kadar abu tidak larut asam

% kadar abu tidak larut asam 

berat abu tidak larut asam
 100%
berat sampel

No.

Berat sampel
(g)

Berat krus

Berat krus + abu

Berat abu tidak
larut asam

1

2,0938

63,5387

63,5479

0,0092

2

2,0082

58,2095

58,2193

0,0098

3

2,0179

57,2289

57,2385

0,0096

1. Berat sampel

= 2,0938 g

Berat abu

= 0,0092 g

Kadar abu

=

0,0092
 100%  0,4394%
2,0938

2. Berat sampel

= 2,0082 g

Berat abu

= 0,0098 g

Kadar abu

=

0,0098
 100%  0,4880%
2,0082

3. Berat sampel

= 2,0179 g

Berat abu

= 0,0096 g

Kadar abu

=

0,0096
 100%  0,4757%
2,0179

Rata-rata kadar abu tidak larut asam =

0,4394  0,4880  0,4757
 0,4677%
3

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Lampiran 10. Hasil analisis data SPSS berat badan mencit
a. Berat badan mula-mula
Descriptives

Kontrol
5 mg/ kg bb
50 mg/ kg bb
300 mg/ kg bb
1000 mg/ kg bb
2000 mg/ kg bb
4000 mg/ kg bb
8000 mg/ kg bb
Total

N

Mean

Std.
Deviatio
n

5
5
5
5
5
5
5
5
40

28.7700
28.9600
29.1300
29.6840
28.8480
29.4140
30.9340
30.5800
29.5400

2.20474
1.10939
1.97046
.73982
1.83089
.87183
1.31466
.82601
1.53060

Std.
Error
.98599
.49614
.88122
.33086
.81880
.38989
.58793
.36940
.24201

95% Confidence
Interval for Mean
Lower
Upper
Bound
Bound
26.0324
31.5076
27.5825
30.3375
26.6834
31.5766
28.7654
30.6026
26.5746
31.1214
28.3315
30.4965
29.3016
32.5664
29.5544
31.6056
29.0505
30.0295

Min

Max

5
5
5
5
5
5
5
5
40

28.7700
28.9600
29.1300
29.6840
28.8480
29.4140
30.9340
30.5800
29.5400

Test of Homogeneity of Variances
Levene Statistic
df1
df2
Sig.
2.098
7
32
.073
Tukey
(I) perlakuan

kontrol

dosis 5 mg/
kg bb

dosis 50 mg/
kg bb

dosis 300
mg/ kg bb

(J) perlakuan
dosis 5 mg/ kg bb
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 5 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 5 mg/ kg bb
dosis 50 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb

Mean
Difference
(I-J)
-.19000
-.36000
-.91400
-.07800
-.64400
-2.16400
-1.81000
.19000
-.17000
-.72400
.11200
-.45400
-1.97400
-1.62000
.36000
.17000
-.55400
.28200
-.28400
-1.80400
-1.45000
.91400
.72400
.55400
.83600
.27000
-1.25000

Std.
Error

Sig.

.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316

1.000
1.000
.973
1.000
.996
.302
.522
1.000
1.000
.993
1.000
1.000
.414
.653
1.000
1.000
.999
1.000
1.000
.526
.763
.973
.993
.999
.983
1.000
.871

95% Confidence
Interval
Lower
Upper
Bound
Bound
-3.1804
2.8004
-3.3504
2.6304
-3.9044
2.0764
-3.0684
2.9124
-3.6344
2.3464
-5.1544
.8264
-4.8004
1.1804
-2.8004
3.1804
-3.1604
2.8204
-3.7144
2.2664
-2.8784
3.1024
-3.4444
2.5364
-4.9644
1.0164
-4.6104
1.3704
-2.6304
3.3504
-2.8204
3.1604
-3.5444
2.4364
-2.7084
3.2724
-3.2744
2.7064
-4.7944
1.1864
-4.4404
1.5404
-2.0764
3.9044
-2.2664
3.7144
-2.4364
3.5444
-2.1544
3.8264
-2.7204
3.2604
-4.2404
1.7404

Universitas Sumatera Utara

Lampiran 10. (Lanjutan)
onfide
(I) perlakuan

(J) perlakuan

r

29.5200 dosis 1000
29.8500

mg/ kg bb

29.7150
30.6110
29.7550
30.5970 dosis 2000
mg/ kg bb

31.1540
31.2650
40 30.3084
dosis 4000
mg/ kg bb

5

dosis 8000
mg/ kg bb

oups
oups

dosis 8000 mg/ kg bb
kontrol
dosis 5 mg/ kg bb
.48461
28.1745
dosis 50 mg/ kg bb
dosis 300 mg/
kg bb
.64020
28.0725
dosis 2000 mg/ kg bb
dosis
4000 28.2949
mg/ kg bb
.51149
dosis 8000 mg/ kg bb
.60183kontrol
28.9401
dosis 5 mg/ kg bb
.65056 27.9488
dosis 50 mg/ kg bb
dosis 300 mg/
kg bb
.51271
29.1735
dosis 1000 mg/ kg bb
.61529
dosis
4000 29.4457
mg/ kg bb
dosis 8000 mg/ kg bb
.42772kontrol
30.0775
dosis 5 mg/ kg bb
.20693
29.8898
dosis 50 mg/ kg BB
dosis 300 mg/ kg BB
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 8000 mg/ kg bb
Kontrol
dosis 5 mg/ kg bb
kg bb
7dosis 50 mg/32
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb

16.475

7

2.354

50.323

32

1.573

66.798

39

Mean
Difference
(I-J)

Std.
Error

Sig.

.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316
.92316

.975
1.000
1.000
1.000
.983
.998
.346
.576
.996
1.000
1.000
1.000
.998
.720
.906
.302
.414
.526
.871
.346
.720
1.000
.522
.653
.763
.975
.576
.906
1.000

Uppe

-.89600
.07800
-.11200
30.8655
-.28200
-.83600
31.6275
-.56600
-2.08600
31.1351
-1.73200
32.2819
.64400
.45400
31.5612
.28400
-.27000
32.0205
.56600
32.8623
-1.52000
-1.16600
32.4525
2.16400
1.97400
30.7269
1.80400
1.25000
2.08600
1.52000
.35400
1.81000
1.62000
.979 1.45000
.89600
1.73200
1.16600
-.35400

95% Confidence
Interval
Lower
Upper
Bound
Bound
-3.8864
2.0944
-2.9124
3.0684
-3.1024
2.8784
-3.2724
2.7084
-3.8264
2.1544
-3.5564
2.4244
-5.0764
.9044
-4.7224
1.2584
-2.3464
3.6344
-2.5364
3.4444
-2.7064
3.2744
-3.2604
2.7204
-2.4244
3.5564
-4.5104
1.4704
-4.1564
1.8244
-.8264
5.1544
-1.0164
4.9644
-1.1864
4.7944
-1.7404
4.2404
-.9044
5.0764
-1.4704
4.5104
-2.6364
3.3444
-1.1804
4.8004
-1.3704
4.6104
-1.5404
4.4404
-2.0944
3.8864
-1.2584
4.7224
-1.8244
4.1564
-3.3444
2.6364

Universitas Sumatera Utara

Lampiran 10. (Lanjutan)
b. Berat badan minggu I
Descriptives

Kontrol
5 mg/ kg bb
50 mg/ kg bb
300 mg/ kg bb
1000 mg/ kg bb
2000 mg/ kg bb
4000 mg/ kg bb
8000 mg/ kg bb
Total

N

Mean

Std.
Deviation

5
5
5
5
5
5
5
5
40

29.5200
29.8500
29.7150
30.6110
29.7550
30.5970
31.1540
31.2650
30.3084

1.08361
1.43153
1.14373
1.34573
1.45470
1.14645
1.37583
.95641
1.30873

Std.
Error
.48461
.64020
.51149
.60183
.65056
.51271
.61529
.42772
.20693

95% Confidence
Interval for Mean
Lower
Upper
Bound
Bound
28.1745
30.8655
28.0725
31.6275
28.2949
31.1351
28.9401
32.2819
27.9488
31.5612
29.1735
32.0205
29.4457
32.8623
30.0775
32.4525
29.8898
30.7269

Min

Max

28.05
28.17
28.17
29.17
28.49
29.15
29.05
29.97
28.05

30.74
31.67
31.29
32.66
32.15
32.27
32.66
32.56
32.66

Test of Homogeneity of Variances
Levene Statistic
df1
df2
Sig.
.215
7
32
.979
Tukey
(I) perlakuan

kontrol

dosis 5 mg/
kg bb

dosis 50 mg/
kg bb

dosis 300
mg/ kg bb

(J) perlakuan

Mean
Difference
(I-J)

Std.
Error

Sig.

dosis 5 mg/ kg bb
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 5 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 5 mg/ kg bb
dosis 50 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb

-.33000
-.19500
-1.09100
-.23500
-1.07700
-1.63400
-1.74500
.33000
.13500
-.76100
.09500
-.74700
-1.30400
-1.41500
.19500
-.13500
-.89600
-.04000
-.88200
-1.43900
-1.55000
1.09100
.76100
.89600
.85600
.01400
-.54300

.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312

1.000
1.000
.861
1.000
.869
.460
.378
1.000
1.000
.977
1.000
.979
.721
.635
1.000
1.000
.945
1.000
.949
.615
.526
.861
.977
.945
.957
1.000
.997

95% Confidence
Interval
Lower
Upper
Bound
Bound
-2.8991 2.2391
-2.7641 2.3741
-3.6601 1.4781
-2.8041 2.3341
-3.6461 1.4921
-4.2031
.9351
-4.3141
.8241
-2.2391 2.8991
-2.4341 2.7041
-3.3301 1.8081
-2.4741 2.6641
-3.3161 1.8221
-3.8731 1.2651
-3.9841 1.1541
-2.3741 2.7641
-2.7041 2.4341
-3.4651 1.6731
-2.6091 2.5291
-3.4511 1.6871
-4.0081 1.1301
-4.1191 1.0191
-1.4781 3.6601
-1.8081 3.3301
-1.6731 3.4651
-1.7131 3.4251
-2.5551 2.5831
-3.1121 2.0261

Universitas Sumatera Utara

Lampiran 10. (Lanjutan)
onfide
(I) perlakuan

(J) perlakuan

r

29.5200 dosis 1000
mg/ kg bb

29.8500
29.7150
30.6110
50
2000
30.5970 dosis
mg/ kg bb
31.1540

31.2650
40 30.3084
dosis 4000
mg/ kg bb

5 8000
dosis
mg/ kg bb

oups

Mean
Difference
(I-J)

Uppe

dosis 8000 mg/ kg bb
-.65400
kontrol
.23500
dosis 5 mg/ kg bb
-.09500
4615028.1745
dosis
mg/ kg bb 30.8655
.04000
dosis 300 mg/ kg bb
-.85600
.64020
28.0725 31.6275
dosis 2000 mg/ kg bb
-.84200
dosis
4000
mg/
kg
bb
-1.39900
.51149 28.2949 31.1351
dosis 8000 mg/ kg bb
-1.51000
.60183 kontrol
28.9401 32.2819
1.07700
dosis 5 mg/ kg bb
.74700
.65056
dosis 5027.9488
mg/ kg bb 31.5612
.88200
dosis 300 mg/ kg bb
-.01400
.51271 29.1735 32.0205
dosis 1000 mg/ kg bb
.84200
dosis
4000
mg/
kg
bb
-.55700
.61529 29.4457 32.8623
dosis 8000 mg/ kg bb
-.66800
.42772 kontrol
30.0775 32.4525
1.63400
dosis 5 mg/ kg bb
1.30400
.20693
29.8898
30.7269
dosis 50 mg/ kg BB
1.43900
dosis 300 mg/ kg BB
.54300
dosis 1000 mg/ kg bb
1.39900
dosis 2000 mg/ kg bb
.55700
dosis 8000 mg/ kg bb
-.11100
Kontrol
1.74500
dosis 5 mg/ kg bb
1.41500
dosis
50
mg/
kg
bb
7
32
.979 1.55000
dosis 300 mg/ kg bb
.65400
dosis 1000 mg/ kg bb
1.51000
dosis 2000 mg/ kg bb
.66800
dosis 4000 mg/ kg bb
.11100

16.475

7

2.354

50.323

32

573

66.798

39

Std.
Error

Sig.

.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312
.79312

.990
1.000
1.000
1.000
.957
.960
.647
.558
.869
.979
.949
1.000
.960
.996
.989
.460
.721
.615
.997
.647
.996
1.000
.378
.635
.526
.990
.558
.989
1.000

95% Confidence
Interval
Lower
Upper
Bound
Bound
-3.2231 1.9151
-2.3341 2.8041
-2.6641 2.4741
-2.5291 2.6091
-3.4251 1.7131
-3.4111 1.7271
-3.9681 1.1701
-4.0791 1.0591
-1.4921 3.6461
-1.8221 3.3161
-1.6871 3.4511
-2.5831 2.5551
-1.7271 3.4111
-3.1261 2.0121
-3.2371 1.9011
-.9351
4.2031
-1.2651 3.8731
-1.1301 4.0081
-2.0261 3.1121
-1.1701 3.9681
-2.0121 3.1261
-2.6801 2.4581
-.8241
4.3141
-1.1541 3.9841
-1.0191 4.1191
-1.9151 3.2231
-1.0591 4.0791
-1.9011 3.2371
-2.4581 2.6801

Universitas Sumatera Utara

Lampiran 10. (Lanjutan)
c. Berat badan minggu 2
Descriptives

Kontrol
5 mg/ kg bb
50 mg/ kg bb
300 mg/ kg bb
1000 mg/ kg bb
2000 mg/ kg bb
4000 mg/ kg bb
8000 mg/ kg bb
Total

N

Mean

Std.
Deviation

5
5
5
5
5
5
5
5
40

29.9450
30.9500
30.5600
32.0220
31.5760
31.5610
32.8300
32.4900
31.4918

1.05414
1.19435
.94260
1.29405
1.12055
1.19153
1.10909
.62230
1.34686

Std.
Error
.47143
.53413
.42154
.57872
.50112
.53287
.49600
.27830
.21296

95% Confidence
Interval for Mean
Lower
Upper
Bound
Bound
.47143 28.6361
.53413 29.4670
.42154 29.3896
.57872 30.4152
.50112 30.1847
.53287 30.0815
.49600 31.4529
.27830 31.7173
.21296 31.0610

Min

Max

31.2539
32.4330
31.7304
33.6288
32.9673
33.0405
34.2071
33.2627
31.9225

28.98
29.57
29.58
30.13
29.84
30.15
31.33
31.68
28.98

Test of Homogeneity of Variances
Levene
df1
df2
Sig.
Statistic
.483
7
32
.840
Tukey
(I) perlakuan

kontrol

dosis 5 mg/
kg bb

dosis 50 mg/
kg bb

dosis 300
mg/ kg bb

(J) perlakuan

Mean
Difference (IJ)

Std.
Error

Sig.

dosis 5 mg/ kg bb
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 5 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 5 mg/ kg bb
dosis 50 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb

-1.00500
-.61500
-2.07700
-1.63100
-1.61600
-2.88500
-2.54500
1.00500
.39000
-1.07200
-.62600
-.61100
-1.88000
-1.54000
.61500
-.39000
-1.46200
-1.01600
-1.00100
-2.27000*
-1.93000
2.07700
1.07200
1.46200
.44600
.46100
-.80800

.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533

.819
.984
.080
.285
.295
.360
.320
.819
.999
.767
.983
.985
.146
.352
.984
.999
.417
.811
.822
.042
.126
.080
.767
.417
.998
.997
.932

95% Confidence
Interval
Lower
Upper
Bound Bound
-3.2250 1.2150
-2.8350 1.6050
-4.2970 .1430
-3.8510 .5890
-3.8360 .6040
-5.1050 -.6650
-4.7650 -.3250
-1.2150 3.2250
-1.8300 2.6100
-3.2920 1.1480
-2.8460 1.5940
-2.8310 1.6090
-4.1000 .3400
-3.7600 .6800
-1.6050 2.8350
-2.6100 1.8300
-3.6820 .7580
-3.2360 1.2040
-3.2210 1.2190
-4.4900 -.0500
-4.1500 .2900
-.1430 4.2970
-1.1480 3.2920
-.7580 3.6820
-1.7740 2.6660
-1.7590 2.6810
-3.0280 1.4120

Universitas Sumatera Utara

Lampiran 10. (Lanjutan)
onfide
(I) perlakuan

Mean
Difference (IJ)

(J) perlakuan

r

29.5200 dosis 1000
mg/ kg bb

29.8500
29.7150
30.6110
29.7550
2000
.5970 dosis
mg/ kg bb
31.1540

31.2650
40 30.3084
dosis 4000
mg/ kg bb

5 8000
dosis
mg/ kg bb

oups
oups

dosis 8000 mg/ kg bb
kontrol
dosis 5 mg/ kg bb
.48461
28.1745
dosis 50 mg/
kg bb
dosis 300 mg/ kg bb
020 28.0725
dosis 2000 mg/ kg bb
dosis 400028.2949
mg/ kg bb
.51149
dosis 8000 mg/ kg bb
.60183kontrol
28.9401
dosis 5 mg/ kg bb
.65056
27.9488
dosis 50 mg/
kg bb
dosis 300 mg/ kg bb
.51271 29.1735
dosis 1000 mg/ kg bb
dosis 400029.4457
mg/ kg bb
.61529
dosis 8000 mg/ kg bb
.42772kontrol
30.0775
dosis 5 mg/ kg bb
.20693
29.8898
dosis 50 mg/
kg BB
dosis 300 mg/ kg BB
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 8000 mg/ kg bb
Kontrol
dosis 5 mg/ kg bb
kg bb
7dosis 50 mg/32
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb

16.475

7

2.354

50.323

32

1.573

66.798

39

Uppe

-.46800
1.63100
.62600
30.8655
1.01600
-.44600
31.6275
.01500
-1.25400
31.1351
-.91400
32.2819
1.61600
.61100
31.5612
1.00100
-.46100
32.0205
-.01500
-1.26900
32.8623
-.92900
32.4525
-1.88500
1.88000
30.7269
-1.27000
.80800
1.25400
1.26900
.34000
-1.54500
1.54000
1.93000
.979
.46800
.91400
.92900
-.34000

Std.
Error

Sig.

.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533

.997
.285
.983
.811
.998
1.000
.606
.879
.295
.985
.822
.997
1.000
.592
.870
.360
.146
.420
.932
.606
.592
1.000
.320
.352
.126
.997
.879
.870
1.000

95% Confidence
Interval
Lower
Upper
Bound Bound
-2.6880 1.7520
-.5890 3.8510
-1.5940 2.8460
-1.2040 3.2360
-2.6660 1.7740
-2.2050 2.2350
-3.4740 .9660
-3.1340 1.3060
-.6040 3.8360
-1.6090 2.8310
-1.2190 3.2210
-2.6810 1.7590
-2.2350 2.2050
-3.4890 .9510
-3.1490 1.2910
.6650
5.1050
-.3400 4.1000
.0500
4.4900
-1.4120 3.0280
-.9660 3.4740
-.9510 3.4890
-1.8800 2.5600
.3250
4.7650
-.6800 3.7600
-.2900 4.1500
-1.7520 2.6880
-1.3060 3.1340
-1.2910 3.1490
-2.5600 1.8800

Universitas Sumatera Utara

Lampiran 11. Hasil analisis data SPSS konsumsi makanan mencit
a. Berat Makanan minggu I
Descriptives

Kontrol
5 mg/ kg bb
50 mg/ kg bb
300 mg/ kg bb
1000 mg/ kg bb
2000 mg/ kg bb
4000 mg/ kg bb
8000 mg/ kg bb
Total

N

Mean

Std.
Deviation

7
7
7
7
7
7
7
7
56

3.5900
3.6857
3.7057
3.7671
3.8486
3.8286
3.9857
4.0414
3.8066

.12055
.22875
.16390
.41548
.52863
.11127
.18274
.20828
.29997

Std.
Error
.04557
.08646
.06195
.15704
.19980
.04206
.06907
.07872
.04008

95% Confidence
Interval for Mean
Lower
Upper
Bound
Bound
3.4785
3.7015
3.4742
3.8973
3.5541
3.8573
3.3829
4.1514
3.3597
4.3375
3.7257
3.9315
3.8167
4.1547
3.8488
4.2341
3.7263
3.8869

Min

Max

3.46
3.41
3.53
3.16
3.28
3.70
3.70
3.79
3.16

7
7
7
7
7
7
7
7
56

ANOVA

Between
Groups
Within Groups
Total

Sum of
Squares

df

Mean Square

F

Sig.

1.139

7

.163

2.050

.068

3.810
4.949

48
55

.079

Tukey
(I) perlakuan

kontrol

dosis 5 mg/
kg bb

dosis 50 mg/
kg bb

dosis 300
mg/ kg bb

(J) perlakuan

Mean
Difference
(I-J)

Std.
Error

Sig.

dosis 5 mg/ kg bb
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
Kontrol
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
Kontrol
dosis 5 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
Kontrol
dosis 5 mg/ kg bb

-.09571
-.11571
-.17714
-.25857
-.23857
-.39571
-.45143
.09571
-.02000
-.08143
-.16286
-.14286
-.30000
-.35571
.11571
.02000
-.06143
-.14286
-.12286
-.28000
-.33571
.17714
.08143

.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059

.998
.994
.935
.676
.757
.171
.076
.998
1.000
.999
.958
.979
.498
.283
.994
1.000
1.000
.979
.991
.584
.353
.935
.999

95% Confidence
Interval
Lower
Upper
Bound
Bound
-.5728
.3814
-.5928
.3614
-.6542
.3000
-.7357
.2185
-.7157
.2385
-.8728
.0814
-.9285
.0257
-.3814
.5728
-.4971
.4571
-.5585
.3957
-.6400
.3142
-.6200
.3342
-.7771
.1771
-.8328
.1214
-.3614
.5928
-.4571
.4971
-.5385
.4157
-.6200
.3342
-.6000
.3542
-.7571
.1971
-.8128
.1414
-.3000
.6542
-.3957
.5585

Universitas Sumatera Utara

Lampiran 11. (Lanjutan)
onfide
(I) perlakuan

Mean
Difference
(I-J)

(J) perlakuan

r

29.5200
29.8500
29.7150 dosis 1000
mg/ kg bb
30.6110
29.7550
30.5970
31.1540
31.2650 dosis 2000
40 30.3084

mg/ kg bb

dosis 4000
mg/ kg bb

5

dosis 8000
mg/ kg bb

oups
oups

dosis 50 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
.48461
dosis 400028.1745
mg/ kg bb
dosis 8000 mg/ kg bb
.64020kontrol
28.0725
dosis
kg bb
11495 mg/
28.2949
dosis 50 mg/ kg bb
dosis 300 28.9401
mg/ kg bb
.60183
dosis 2000 mg/ kg bb
.65056
dosis 400027.9488
mg/ kg bb
dosis 8000 mg/ kg bb
.51271 29.1735
kontrol
dosis
kg bb
.615295 mg/
29.4457
dosis 50 mg/ kg bb
.42772
dosis 300 30.0775
mg/ kg bb
dosis 1000 mg/ kg bb
.20693
dosis 400029.8898
mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 5 mg/ kg bb
dosis 50 mg/ kg BB
dosis 300 mg/ kg BB
dosis 1000 mg/ kg bb
dosis
7 2000 mg/2kg bb
dosis 8000 mg/ kg bb
Kontrol
dosis 5 mg/ kg bb
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb

16.475

7

2.354

50.323

32

1.573

66.798

39

Uppe

.06143
-.08143
-.06143
30.8655
-.21857
-.27429
31.6275
.25857
.16286
31.1351
.14286
.08143
32.2819
.02000
31.5612
-.13714
-.19286
32.0205
.23857
.14286
32.8623
.12286
32.4525
.06143
-.02000
30.7269
-.15714
-.21286
.39571
.30000
.28000
.21857
.13714
.979.15714
-.05571
.45143
.35571
.33571
.27429
.19286
.21286
.05571

Std.
Error

Sig.

.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059
.15059

1.000
.999
1.000
.828
.609
.676
.958
.979
.999
1.000
.984
.902
.757
.979
.991
1.000
1.000
.965
.847
.171
.498
.584
.828
.984
.965
1.000
.076
.283
.353
.609
.902
.847
1.000

95% Confidence
Interval
Lower
Upper
Bound
Bound
-.4157
.5385
-.5585
.3957
-.5385
.4157
-.6957
.2585
-.7514
.2028
-.2185
.7357
-.3142
.6400
-.3342
.6200
-.3957
.5585
-.4571
.4971
-.6142
.3400
-.6700
.2842
-.2385
.7157
-.3342
.6200
-.3542
.6000
-.4157
.5385
-.4971
.4571
-.6342
.3200
-.6900
.2642
-.0814
.8728
-.1771
.7771
-.1971
.7571
-.2585
.6957
-.3400
.6142
-.3200
.6342
-.5328
.4214
-.0257
.9285
-.1214
.8328
-.1414
.8128
-.2028
.7514
-.2842
.6700
-.2642
.6900
-.4214
.5328

Universitas Sumatera Utara

Lampiran 11. (Lanjutan)
b. Berat makanan minggu II
Descriptives

Kontrol
5 mg/ kg bb
50 mg/ kg bb
300 mg/ kg bb
1000 mg/ kg bb
2000 mg/ kg bb
4000 mg/ kg bb
8000 mg/ kg bb
Total

N

Mean

7
7
7
7
7
7
7
7
56

3.6986
3.7529
3.8900
3.9129
4.0200
4.1300
4.2129
4.3357
3.9941

Std.
Deviation
.68943
.81418
.52767
.52175
.52249
.10456
.11116
.07569
.51118

Std.
Error
.26058
.30773
.19944
.19720
.19748
.03952
.04202
.02861
.06831

95% Confidence
Interval for Mean
Lower
Upper
Bound
Bound
3.0610
4.3362
2.9999
4.5058
3.4020
4.3780
3.4303
4.3954
3.5368
4.5032
4.0333
4.2267
4.1100
4.3157
4.2657
4.4057
3.8572
4.1310

Min

Max

3.08
3.06
3.27
3.31
3.23
3.93
3.99
4.23
3.06

4.66
4.89
4.50
4.68
4.60
4.24
4.32
4.45
4.89

ANOVA
Sum of
Squares
Between
Groups
Within Groups
Total

df

Mean Square

2.427

7

.347

11.945
14.372

48
55

.249

F
1.393

Sig.
.230

Tukey
(I) perlakuan

kontrol

dosis 5 mg/
kg bb

dosis 50 mg/
kg bb

dosis 300
mg/ kg bb

(J) perlakuan

Mean
Difference
(I-J)

Std.
Error

Sig.

dosis 5 mg/ kg bb
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
Kontrol
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
Kontrol
dosis 5 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
Kontrol
dosis 5 mg/ kg bb

-1.00500
-.61500
-2.07700
-1.63100
-1.61600
-2.88500
-2.54500
1.00500
.39000
-1.07200
-.62600
-.61100
-1.88000
-1.54000
.61500
-.39000
-1.46200
-1.01600
-1.00100
-1.27000
-1.93000
2.07700
1.07200

.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533

.819
.984
.080
.285
.295
.360
.320
.819
.999
.767
.983
.985
.146
.352
.984
.999
.417
.811
.822
.420
.126
.080
.767

95% Confidence
Interval
Lower
Upper
Bound
Bound
-3.2250
1.2150
-2.8350
1.6050
-4.2970
.1430
-3.8510
.5890
-3.8360
.6040
-5.1050
-.6650
-4.7650
-.3250
-1.2150
3.2250
-1.8300
2.6100
-3.2920
1.1480
-2.8460
1.5940
-2.8310
1.6090
-4.1000
.3400
-3.7600
.6800
-1.6050
2.8350
-2.6100
1.8300
-3.6820
.7580
-3.2360
1.2040
-3.2210
1.2190
-4.4900
-.0500
-4.1500
.2900
-.1430
4.2970
-1.1480
3.2920

Universitas Sumatera Utara

Lampiran 11. (Lanjutan)
onfide
(I) perlakuan

(J) perlakuan

r

29.5200
29.8500
29.7150

dosis 1000

30.6110 mg/ kg bb
29.7550
30.5970
31.1540
31.2650 dosis 2000
40 30.3084 mg/ kg bb

dosis 4000
mg/ kg bb

5

dosis 8000
mg/ kg bb

oups
oups

dosis 50 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
.48461 28.1745
dosis 4000 mg/ kg bb
dosis
8000 28.0725
mg/ kg bb
.64020
kontrol
.51149
28.2949
dosis 5 mg/
kg bb
dosis 50 mg/ kg bb
.60183
28.9401
dosis 300 mg/
kg bb
dosis
2000 27.9488
mg/ kg bb
.65056
dosis 4000 mg/ kg bb
dosis
8000 29.1735
mg/ kg bb
.51271
kontrol
.61529
29.4457
dosis 5 mg/
kg bb
dosis 50 mg/ kg bb
.42772
30.0775
dosis 300 mg/ kg bb
dosis
1000 29.8898
mg/ kg bb
.20693
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 5 mg/ kg bb
dosis 50 mg/ kg BB
dosis 300 mg/ kg BB
dosis 1000 mg/ kg bb
7 2000 mg/32kg bb
dosis
dosis 8000 mg/ kg bb
Kontrol
dosis 5 mg/ kg bb
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb

16.475

7

2.354

50.323

32

1.573

66.798

39

Mean
Difference
(I-J)

Std.
Error

Sig.

.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533
.68533

.417
.998
.997
.932
.997
.285
.983
.811
.998
1.000
.606
.879
.295
.985
.822
.997
1.000
.592
.870
.360
.146
.420
.932
.606
.592
1.000
.610
.352
.126
.997
.879
.870
1.000

Uppe

1.46200
.44600
.46100
30.8655
-.80800
-.46800
31.6275
1.63100
31.1351
.62600
1.01600
32.2819
-.44600
.01500
31.5612
-1.25400
-.91400
32.0205
1.61600
32.8623
.61100
1.00100
32.4525
-.46100
-.01500
30.7269
-1.26900
-.92900
2.88500
1.88000
2.27000
.80800
1.25400
.979
1.26900
.34000
2.54500
1.54000
1.93000
.46800
.91400
.92900
-.34000

95% Confidence
Interval
Lower
Upper
Bound
Bound
-.7580
3.6820
-1.7740
2.6660
-1.7590
2.6810
-3.0280
1.4120
-2.6880
1.7520
-.5890
3.8510
-1.5940
2.8460
-1.2040
3.2360
-2.6660
1.7740
-2.2050
2.2350
-3.4740
.9660
-3.1340
1.3060
-.6040
3.8360
-1.6090
2.8310
-1.2190
3.2210
-2.6810
1.7590
-2.2350
2.2050
-3.4890
.9510
-3.1490
1.2910
.6650
5.1050
-.3400
4.1000
.0500
4.4900
-1.4120
3.0280
-.9660
3.4740
-.9510
3.4890
-1.8800
2.5600
.3250
4.7650
-.6800
3.7600
-.2900
4.1500
-1.7520
2.6880
-1.3060
3.1340
-1.2910
3.1490
-2.5600
1.8800

Universitas Sumatera Utara

Lampiran 12. Hasil analisis data SPSS konsumsi minuman mencit
a. Volume minuman minggu I
Descriptives

Kontrol
5 mg/ kg bb
50 mg/ kg bb
300 mg/ kg bb
1000 mg/ kg bb
2000 mg/ kg bb
4000 mg/ kg bb
8000 mg/ kg bb
Total

N

Mean

Std.
Deviation

7
7
7
7
7
7
7
7
56

4.5043
4.5400
4.7600
4.9000
4.9329
5.0300
5.0900
5.1100
4.8584

.74355
.90011
.37488
.37709
.37053
.59175
.28042
.37996
.55380

Std.
Error
.28103
.34021
.14169
.14253
.14005
.22366
.10599
.14361
.07400

95% Confidence
Interval for Mean
Lower
Upper
Bound
Bound
3.8166
5.1920
3.7075
5.3725
4.4133
5.1067
4.5512
5.2488
4.5902
5.2755
4.4827
5.5773
4.8307
5.3493
4.7586
5.4614
4.7101
5.0067

Min

Max

3.03
3.63
4.40
4.45
4.32
4.09
4.73
4.51
3.03

5.20
5.58
5.27
5.32
5.33
5.61
5.41
5.62
5.62

ANOVA

Between
Groups
Within Groups
Total

Sum of
Squares

df

Mean Square

F

Sig.

2.731

7

.390

1.325

.260

14.138
16.868

48
55

.295

Tukey
(I) perlakuan

kontrol

dosis 5 mg/
kg bb

dosis 50 mg/
kg bb

dosis 300
mg/ kg bb

(J) perlakuan

Mean
Difference
(I-J)

Std.
Error

Sig.

dosis 5 mg/ kg bb
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
Kontrol
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 5 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 5 mg/ kg bb

-.03571
-.25571
-.39571
-.42857
-.52571
-.58571
-.60571
.03571
-.22000
-.36000
-.39286
-.49000
-.55000
-.57000
.25571
.22000
-.14000
-.17286
-.27000
-.33000
-.35000
.39571
.36000

.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009

1.000
.986
.869
.815
.615
.480
.437
1.000
.994
.915
.873
.694
.560
.515
.986
.994
1.000
.999
.981
.945
.926
.869
.915

95% Confidence
Interval
Lower
Upper
Bound
Bound
-.9548
.8834
-1.1748
.6634
-1.3148
.5234
-1.3477
.4905
-1.4448
.3934
-1.5048
.3334
-1.5248
.3134
-.8834
.9548
-1.1391
.6991
-1.2791
.5591
-1.3119
.5262
-1.4091
.4291
-1.4691
.3691
-1.4891
.3491
-.6634
1.1748
-.6991
1.1391
-1.0591
.7791
-1.0919
.7462
-1.1891
.6491
-1.2491
.5891
-1.2691
.5691
-.5234
1.3148
-.5591
1.2791

Universitas Sumatera Utara

Lampiran 12. (Lanjutan)
onfide
(I) perlakuan

Mean
Difference
(I-J)

(J) perlakuan

r

29.5200
29.8500
29.7150 dosis 1000
30.6110 mg/ kg bb
29.7550
30.5970
31.1540
31.2650 dosis 2000
0 30.3084

mg/ kg bb

dosis 4000
mg/ kg bb

5

dosis 8000
mg/ kg bb

oups
oups

Uppe

dosis 50 mg/ kg bb
.14000
dosis 1000 mg/ kg bb
-.03286
dosis 2000 mg/ kg bb
-.13000
.48461
dosis 400028.1745
mg/ kg bb 30.8655
-.19000
dosis 8000 mg/ kg bb
-.21000
.64020kontrol
28.0725 31.6275
.42857
dosis
5
mg/
kg
bb
.39286
.51149 28.2949 31.1351
dosis 50 mg/ kg bb
.17286
.60183
dosis 30028.9401
mg/ kg bb 32.2819
.03286
dosis 2000 mg/ kg bb
-.09714
.65056
dosis 400027.9488
mg/ kg bb 31.5612
-.15714
dosis 8000 mg/ kg bb
-.17714
.51271 29.1735 32.0205
kontrol
.52571
dosis
5
mg/
kg
bb
.49000
.61529 29.4457 32.8623
dosis 50 mg/ kg bb
.27000
.42772
dosis 30030.0775
mg/ kg bb 32.4525
.13000
dosis 1000 mg/ kg bb
.09714
.20693
29.8898
30.7269
dosis 4000 mg/ kg bb
-.06000
dosis 8000 mg/ kg bb
-.08000
kontrol
.58571
dosis 5 mg/ kg bb
.55000
dosis 50 mg/ kg BB
.33000
dosis 300 mg/ kg BB
.19000
dosis 1000 mg/ kg bb
.15714
dosis
2000
mg/
kg
bb
7
32
.979.06000
dosis 8000 mg/ kg bb
-.02000
Kontrol
.60571
dosis 5 mg/ kg bb
.57000
dosis 50 mg/ kg bb
.35000
dosis 300 mg/ kg bb
.21000
dosis 1000 mg/ kg bb
.17714
dosis 2000 mg/ kg bb
.08000
dosis 4000 mg/ kg bb
.02000

16.475

7

2.354

50.323

32

1.573

66.798

39

Std.
Error

Sig.

.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009
.29009

1.000
1.000
1.000
.998
.996
.815
.873
.999
1.000
1.000
.999
.999
.615
.694
.981
1.000
1.000
1.000
1.000
.480
.560
.945
.998
.999
1.000
1.000
.437
.515
.926
.996
.999
1.000
1.000

95% Confidence
Interval
Lower
Upper
Bound
Bound
-.7791
1.0591
-.9519
.8862
-1.0491
.7891
-1.1091
.7291
-1.1291
.7091
-.4905
1.3477
-.5262
1.3119
-.7462
1.0919
-.8862
.9519
-1.0162
.8219
-1.0762
.7619
-1.0962
.7419
-.3934
1.4448
-.4291
1.4091
-.6491
1.1891
-.7891
1.0491
-.8219
1.0162
-.9791
.8591
-.9991
.8391
-.3334
1.5048
-.3691
1.4691
-.5891
1.2491
-.7291
1.1091
-.7619
1.0762
-.8591
.9791
-.9391
.8991
-.3134
1.5248
-.3491
1.4891
-.5691
1.2691
-.7091
1.1291
-.7419
1.0962
-.8391
.9991
-.8991
.9391

Universitas Sumatera Utara

Lampiran 12. (Lanjutan)
b. Volume minuman minggu II
Descriptives
Std.
Error

95% Confidence
Interval for Mean
Lower
Upper
Bound
Bound

N

Mean

Std.
Deviation

Kontrol

7

4.8986

.42128

.15923

4.5089

7

4.8986 .42128

5 mg/ kg bb

7

5.0586

.40523

.15316

4.6838

7

5.0586 .40523

50 mg/ kg bb

7

5.1000

.39226

.14826

4.7372

7

5.1000 .39226

300 mg/ kg bb

7

5.1343

.36837

.13923

4.7936

7

5.1343 .36837

1000 mg/ kg bb

7

5.1657

.23387

.08839

4.9494

7

5.1657 .23387

2000 mg/ kg bb

7

5.2357

.28053

.10603

4.9763

7

5.2357 .28053

4000 mg/ kg bb

7

5.3643

.13176

.04980

5.2424

7

5.3643 .13176

8000 mg/ kg bb

7

5.3586

.16708

.06315

5.2041

7

5.3586 .16708

Total

56

5.1645

.33159

.04431

5.0757

56

5.1645 .33159

Min

Max

ANOVA

Between
Groups
Within Groups
Total

Sum of
Squares

df

Mean Square

F

Sig.

1.188

7

.170

1.676

.138

4.860
6.047

48
55

.101

Tukey
(I) perlakuan

kontrol

dosis 5 mg/
kg bb

dosis 50 mg/
kg bb

(J) perlakuan

Mean
Difference (IJ)

Std.
Error

Sig.

dosis 5 mg/ kg bb
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 5 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb

-.16000
-.20143
-.23571
-.26714
-.33714
-.46571
-.46000
.16000
-.04143
-.07571
-.10714
-.17714
-.30571
-.30000
.20143
.04143
-.03429
-.06571
-.13571

.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008

.980
.933
.859
.765
.504
.136
.146
.980
1.000
1.000
.998
.965
.625
.646
.933
1.000
1.000
1.000
.992

95% Confidence
Interval
Lower
Upper
Bound
Bound
-.6988
-.16000
-.7403
-.20143
-.7746
-.23571
-.8060
-.26714
-.8760
-.33714
-1.0046
-.46571
-.9988
-.46000
-.3788
.16000
-.5803
-.04143
-.6146
-.07571
-.6460
-.10714
-.7160
-.17714
-.8446
-.30571
-.8388
-.30000
-.3374
.20143
-.4974
.04143
-.5731
-.03429
-.6046
-.06571
-.6746
-.13571

Universitas Sumatera Utara

Lampiran 12. (Lanjutan)
onfide
(I) perlakuan

Mean
Difference (IJ)

(J) perlakuan

r

29.5200

dosis 300

29.8500 mg/ kg bb
29.7150
30.6110
29.7550
dosis 1000

0 mg/ kg bb
31.1540
31.2650
40 30.3084
dosis 2000
mg/ kg bb

5
dosis 4000
mg/ kg bb

oups
oups

dosis 8000
mg/ kg bb

16.475
50.323
66.798

dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
.48461
28.1745
dosis 5 mg/
kg bb
dosis 50 mg/ kg bb
.64020
28.0725
dosis
1000 mg/
kg bb
dosis
2000
mg/
kg bb
.51149 28.2949
dosis 4000 mg/ kg bb
dosis
8000 mg/
kg bb
.60183
28.9401
kontrol
.65056
27.9488
dosis 5 mg/
kg bb
dosis 50 mg/ kg bb
.51271 29.1735
dosis 300 mg/ kg bb
dosis
2000 mg/
kg bb
.61529
29.4457
dosis 4000 mg/ kg bb
dosis
8000 mg/
kg bb
.42772
30.0775
kontrol
.20693
29.8898
dosis 5 mg/
kg bb
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis 1000 mg/ kg bb
dosis 4000 mg/ kg bb
dosis 8000 mg/ kg bb
kontrol
dosis 5 mg/ kg bb
7
32
dosis 50 mg/ kg BB
dosis 300 mg/ kg BB
dosis 1000 mg/ kg bb
dosis 2000 mg/ kg bb
dosis 8000 mg/ kg bb
Kontrol
dosis 5 mg/ kg bb
dosis 50 mg/ kg bb
dosis 300 mg/ kg bb
dosis7 1000 mg/2.354
kg bb
dosis 2000 mg/ kg bb
32 4000 mg/1.573
dosis
kg bb

Uppe
-.26429
-.25857
.23571
30.8655
.07571
.03429
31.6275
-.03143
-.10143
31.1351
-.23000
-.22429
32.2819
.26714
31.5612
.10714
.06571
32.0205
.03143
-.07000
32.8623
-.19857
-.19286
32.4525
.33714
30.7269
.17714
.13571
.10143
.07000
-.12857
-.12286
.46571
.30571
.979
.26429
.23000
.19857
.12857
.00571
.46000
.30000
.25857
.22429
.19286
.12286
-.00571

Std.
Error

Sig.

.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008
.17008

.774
.793
.859
1.000
1.000
1.000
.999
.874
.887
.765
.998
1.000
1.000
1.000
.937
.946
.504
.965
.992
.999
1.000
.995
.996
.136
.625
.774
.874
.937
.995
1.000
.146
.646
.793
.887
.946
.996
1.000

95% Confidence
Interval
Lower
Upper
Bound
Bound
-.8031
-.26429
-.7974
-.25857
-.3031
.23571
-.4631
.07571
-.5046
.03429
-.5703
-.03143
-.6403
-.10143
-.7688
-.23000
-.7631
-.22429
-.2717
.26714
-.4317
.10714
-.4731
.06571
-.5074
.03143
-.6088
-.07000
-.7374
-.19857
-.7317
-.19286
-.2017
.33714
-.3617
.17714
-.4031
.13571
-.4374
.10143
-.4688
.07000
-.6674
-.12857
-.6617
-.12286
-.0731
.46571
-.2331
.30571
-.2746
.26429
-.3088
.23000
-.3403
.19857
-.4103
.12857
-.5331
.00571
-.0788
.46000
-.2388
.30000
-.2803
.25857
-.3146
.22429
-.3460
.19286
-.4160
.12286
-.5446
-.00571

39

Universitas Sumatera Utara

Lampiran 13. Gambar makropatologi organ hati dan ginjal mencit
1. Organ hati

CMC Na 1%

EEBAM 5 mg/kg bb

EEBAM 50 mg/kg bb

EEBAM 300 mg/kg bb

EEBAM 1000 mg/kg bb

EEBAM 2000 mg/kg bb

EEBAM 4000 mg/kg bb

EEBAM 8000 mg/kg bb

Universitas Sumatera Utara

Lampiran 13. (Lanjutan)
2. Organ ginjal

CMC Na 1%

EEBAM 5 mg/kg bb

EEBAM 50 mg/kg bb

EEBAM 300 mg/kg bb

EEBAM 1000 mg/kg bb

EEBAM 2000 mg/kg bb

EEBAM 4000 mg/kg bb

EEBAM 8000 mg/kg bb

Universitas Sumatera Utara