Uji Toksisitas Subkronik Ekstrak Etanol Daun Kembang Bulan (Tithonia diversifolia (Hemsley) A.Gray) pada Mencit Jantan Appendix
Lampiran 1. Hasil identifikasi tumbuhan
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Lampiran 2. Gambar tumbuhan dan daun segarkembang bulan (Tithonia
diversifolia (Hemsley) A. Gray)
Tumbuhan kembang bulan
Daun segar tumbuhan kembang bulan
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Lampiran 3.GambarSimplisia dan serbuk simplisia daun kembang bulan
Simplisia daun kembang bulan
Serbuk simplisia daun kembang bulan
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Lampiran 4. Bagan pembuatan ekstrak
Serbuk simplisia
Dimasukkan ke dalam bejana
Ditambah pelarut 5,5 liter etanol 80% (75
bagian)
Dibiarkan selama 5 hari terlindung dari
cahaya sambil sesekali di aduk
Diserkai, diperas
Maserat I
Ampas
Dicuci dengan pelarut
etanol 80% hingga
diperoleh (100 bagian)
Dipindahkan ke dalam
bejana tertutup
Dibiarkan di tempat
sejuk, terlindung dari
cahaya selama 2 hari
Dienap tuangkan dan
di saring
Maserat II
Ampas
Digabung
Dipekatkan dengan alat rotary evaporator 40oC
Dikeringkan dengan alat freeze dryer -40oC
Ekstrak Kental
(71 g)
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Lampiran 5. Bagan alur penelitian uji toksisitas subkronik
EEDKB
`
Hewan Uji
(Mencit)
Kelompok
kontrol (I)
Kelompok
II
Kelompok
III
Kelompok
IV
Kelompok
IV
Diberi
CMC Na
5%
Diberi
EEDKB dosis
250 mg/kg bb
Diberi
EEDKB dosis
500 mg/kg bb
Diberi
EEDKB dosis
750 mg/kg bb
Diberi
EEDKB dosis
1000 mg/kg
bb
Pengamatan 28 Hari
Gejala
toksik
Berat
Badan
Jumlah
pakan
Kematian
Kadar
ALT dan
Kreatinin
Makropa
-tologi
hati dan
ginjal
Histopatologi
hati dan
ginjal
Analisis data
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Lampiran 6. Contoh perhitungan dosis
Berat badan mencit 25 g dengan dosis EEDKB 1000 mg/kg bb
Dosis = 1000 mg/kg bb
= (25 : 1000 kg ) x 1000 mg/kg
= 25 mg
Konsentrasi ekstrak yang dibuat 6% = 6 g/100 mL
= 6000 mg/100 mL
= 60 mg/mL
Jumlah obat yang disuntikkan
= 25 mg : 60 mg/mL
= 0,42 mL
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Lampiran 7. Hasil uji
pendahuluan1. Hasil pengamatan
berat badan
Perlakuan
Kontrol I
Kontrol II
Dosis 250 mg I
Dosis 250 mg II
Dosis 500 mg I
Dosis 500 mg II
Dosis 1000 mg I
Dosis 1000 mg II
Dosis 1500 mg I
Dosis 1500 mg II
Dosis 3000 mg I
Dosis 3000 mg II
0
27,1
26,0
27
26,5
24
25,1
23,5
25,8
24,2
25,2
23
26,3
1
27,2
26,1
27,1
26,5
24
25
23,5
26
24
25
23
26
Berat badan hari ke- (g)
2
3
4
5
26,8 27,0 26,9 27,2
25,9 26,0 26,1 26,2
26,9 26,8 26,8 26,9
26,4 26,7 26,8 27
23,8
24 23,9 23,5
25,1 24,7 25 24,8
24
23,7 23,5 22
25,7 25,4 24,6 25
23
22,2 21
19
24
24,4 24,5 24,2
22,4 22,1 20
18
18
18
18
18
6
7
27,5 27,3
26,5 26,6
27,1 27,3
26,9 26,8
23,6 23,8
25 25,2
22
22
25,1 25,2
Mati
24,1 24
Mati
Mati
2. Hasil perhitungan dan analisa rata-rata berat badan
Hari
0
7
Rata-rata berat badan (g) ± SD
kontrol
26,55 ±0,78
250 mg
26,75±0,35
500 mg
24,55±0,78
1000 mg
24,65±1,63
1500 mg
24,7±0,71
3000 mg
24,65±2,33
26,95±0,49
27,05±0,35
24,5±0,99
23,6±2,26
24,00±
-
3. Hasil pengamatan gejala toksik
Perubahan bulu
Gerak gerik
Jalan
dengan Gelisah
perut
Rontok
Warna
Jalan
mundur
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
+
-
-
+
-
-
-
-
+
+
-
-
+
-
-
-
-
+
Perlakuan
Kejang
Salivasi
Diare
Lemas
Kontrol
Dosis
250 mg
Dosis
500 mg
Dosis
1000 g
Dosis
1500 mg
Dosis
3000 mg
-
-
-
-
-
-
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Lampiran 8. Hasil pengukuran berat badan
1. Berat badan pada minggu pertama
Perlakuan
kontrol I
kontrol II
kontrol III
kontrol IV
kontrol V
Dosis 250 mg I
Dosis 250 mg II
Dosis 250 mg III
Dosis 250 mg IV
Dosis 250 mg V
Dosis 500 mg I
Dosis 500 mg II
Dosis 500 mg III
Dosis 500 mg IV
Dosis 500 mg V
Dosis 750 mg I
Dosis 750 mg II
Dosis 750 mg III
Dosis 750 mg IV
Dosis 750 mg V
Dosis 1000 mg I
Dosis 1000 mg II
Dosis 1000 mg III
Dosis 1000 mg IV
Dosis 1000 mg V
1
23,3
20
21
22,1
26,2
20,2
27,6
24,4
20
26,9
27,8
22,7
23
25,9
22,7
32
27,8
21
25,4
24
25,2
28
26,1
22,4
25
2
24,9
20,1
21
24,1
27,8
22
28
26
20,1
29
30
24,5
25
26,8
25
32,2
30
22,3
27,8
23,4
25
28,8
28,7
21
26,6
Berat badan hari ke- (g)
3
4
5
23,3
23,5
22
20,2
20,1
20
21,2
21,2
21,1
24
25,1
25
28
27,8
28,4
21.5
20,3
21,6
28,5
28,7
29
25,5
25,6
24,6
20,2
20
20,5
28,8
28,9
29
29,8
28,9
31
24
23,8
23,9
24,8
23,9
25
25,8
24,9
25
23,8
22,9
23,5
31,6
31,7
33
30
28,3
29
25
24
23
28
28,9
29
22
22,8
23,7
24,3
22
19
28,9
29,2
30
29
28,9
29
20
20
21
26
25,6
25,7
6
23,5
20
21,5
24
29
21,8
29,1
25
21
28,8
29,8
24
24,7
26,1
23,4
32
28,8
19,5
29
23,5
18,8
31
30
22,2
26
7
24,3
20
22
25
28,7
22
29,3
24,7
20,7
29
30
24
25
25,9
23,6
29
29
20
28,6
23,4
17
30,5
30,3
22,4
26,1
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2. Berat badan pada minggu kedua
Perlakuan
kontrol I
kontrol II
kontrol III
kontrol IV
kontrol V
Dosis 250 mg I
Dosis 250 mg II
Dosis 250 mg III
Dosis 250 mg IV
Dosis 250 mg V
Dosis 500 mg I
Dosis 500 mg II
Dosis 500 mg III
Dosis 500 mg IV
Dosis 500 mg V
Dosis 750 mg I
Dosis 750 mg II
Dosis 750 mg III
Dosis 750 mg IV
Dosis 750 mg V
Dosis 1000 mg I
Dosis 1000 mg II
Dosis 1000 mg III
Dosis 1000 mg IV
Dosis 1000 mg V
1
24
20
21,4
25
29
21
29,6
26
21
28,7
28,4
24
25
26
24
25
30
20
27,6
20
16
30,6
30
22,4
26
2
24
20
21,3
24,5
29
21
30
25,4
20,8
28,5
28,1
22,3
24,5
25
23,8
24
30,2
21,4
24,5
18
15
30,7
30,6
22,1
25,8
Berat badan hari ke- (g)
3
4
5
6
7
23,7
24
23,8
23,6
23,4
20,2
20,1
20,2
20,3
20,7
21,3
21,8
21,4
20,3
20,4
25
24,8
24,9
25,7
26
30
30,3
30,4
30,6
31
20
20
20,5
20,4
20,4
30
29,7
29,5
28,8
30
25,4
25,2
24
23,8
26
21
22
21
21,3
22
28,3
27,8
26,9
27
27
26,8
27
25,8
28
28,2
20
19,4
16,5
18
19
23,6
24
23,7
25
25,2
23,8
23,6
23
24,4
25
24
24,6
24,7
25,3
26
21
20
Mati
29,5
29,6
28,4
30
30,2
21
20,6
21,8
20
21,4
23
25,2
20
27,6
24,5
17
18
16
20
18
15
Mati
31,4
32
30,6
30,7
31,4
31
30
24
Mati
21,6
22,4
22,1
21,6
22
25,6
26
26
25,8
25,6
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3. Berat badan pada minggu ketiga
Perlakuan
kontrol I
kontrol II
kontrol III
kontrol IV
kontrol V
Dosis 250 mg I
Dosis 250 mg II
Dosis 250 mg III
Dosis 250 mg IV
Dosis 250 mg V
Dosis 500 mg I
Dosis 500 mg II
Dosis 500 mg III
Dosis 500 mg IV
Dosis 500 mg V
Dosis 750 mg I
Dosis 750 mg II
Dosis 750 mg III
Dosis 750 mg IV
Dosis 750 mg V
Dosis 1000 mg I
Dosis 1000 mg II
Dosis 1000 mg III
Dosis 1000 mg IV
Dosis 1000 mg V
1
23,6
20,5
20,3
26
31
20,3
30,8
26,4
21,9
27
28,1
19
25
25
26
2
24,3
20,6
20,4
26
31,6
20,4
31,7
26,8
21,8
27,1
27,7
18
25,3
23,8
26
30
23
20,5
13
31
24,5
20,2
32
24
21,9
24,6
22,5
24,7
Berat badan hari ke- (g)
3
4
5
25
24,7
25
20,4
20,8
21
20,2
20,8
20,8
25,8
25,5
26
31,8
32
31,7
20,5
20
20,3
32
31,6
30,5
27
26,7
26,5
21,9
22
21,8
26,7
26,4
26,4
27,6
27,4
27,2
18
18
19
25,7
26
26,5
24,7
25,7
25,6
26,2
26.7
27
Mati
31
29,8
29,9
24,8
23,9
25
20
19
20
Mati
Mati
Mati
Mati
23
22,8
23,6
24,6
25
24,7
6
25
21,3
21
26,4
31,5
20,5
31
26,6
22
26,2
27
19
27
25,8
26,8
7
24,8
21,4
21
27
30
20,4
31,3
26,8
22,1
26,5
27
18,6
26,8
26
27
30
25,1
21,4
31
25,3
22
24
24,3
23,8
25
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4. Berat badan pada minggu keempat
Perlakuan
kontrol I
kontrol II
kontrol III
kontrol IV
kontrol V
Dosis 250 mg I
Dosis 250 mg II
Dosis 250 mg III
Dosis 250 mg IV
Dosis 250 mg V
Dosis 500 mg I
Dosis 500 mg II
Dosis 500 mg III
Dosis 500 mg IV
Dosis 500 mg V
Dosis 750 mg I
Dosis 750 mg II
Dosis 750 mg III
Dosis 750 mg IV
Dosis 750 mg V
Dosis 1000 mg I
Dosis 1000 mg II
Dosis 1000 mg III
Dosis 1000 mg IV
Dosis 1000 mg V
1
22
25
21,7
21
27
20,7
31
26,4
21,3
27
26,4
20
27,3
26
27,5
2
23
25,5
22
20,7
27,7
21
30,6
27
21
26,8
26
19
29
25,5
28
30,8
25,1
22,5
31
25
23,1
23,6
25
24
25,6
Berat badan hari ke- (g)
3
4
5
6
24
25
26
27
25,8
25,8
26
26,6
21,8
21,6
21,6
21,9
20,8
21
21,3
21,6
28
28
28
29
21,2
21,6
21,5
21,8
30,8
31
30,4
30,7
26
27,1
26,5
26,7
21,7
21,8
21,6
22
25,8
26,4
27
26,5
25,7
26,9
26
26,4
17
17
Mati
28,6
28,8
29
28,5
25
26
27
27,6
26,9
26,5
27
27,2
Mati
30,7
30,9
31,5
31,7
25,4
26
25,3
25,6
23
23,6
25
24,7
Mati
Mati
Mati
Mati
23,7
23,8
24
24,7
25,8
26
26,2
26
7
28
27
22
21,8
29
22
31,1
27,3
22
27
26,5
28,3
28
27,5
32
25
24,5
25
26
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Lampiran 9. Hasil pengukuran kadar ALT dan kreatinin
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Lampiran 9. (lanjutan)
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Lampiran 9.(lanjutan)
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Lampiran 9.(lanjutan)
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Lampiran 9.(lanjutan)
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Lampiran 9.(lanjutan)
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Lampiran 9. (lanjutan)
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Lampiran 9.(lanjutan)
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Lampiran 9.(lanjutan)
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Lampiran 9.(lanjutan)
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Lampiran 9.(lanjutan)
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Lampiran 9.(lanjutan)
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Lampiran 9.(lanjutan)
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Lampiran 9.(lanjutan)
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Lampiran 9.(lanjutan)
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Lampiran 9.(lanjutan)
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Lampiran 9.(lanjutan)
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Lampiran 10. Hasil rata-rata kadar ALT dan kreatinin
1. Hasil rata-rata kadar ALT
Perlakuan
Rata-Rata ±SD
Nilai signifikansi
Kontrol
24,26 ±7,67
-
Dosis 250 mg/kg bb
48,5 ± 7,00
0,09*
Dosis 500 mg/kg bb
73,5 ± 19,46
0,014*
Dosis 750 mg/kg bb
99,5 ± 12,99
0,002*
Dosis 1000 mg/kg bb
151,2 ± 0,80
0,024*
Perlakuan
Rata-Rata ±SD
Nilai signifikansi
Kontrol
0,12 ± 0,05
-
Dosis 250 mg/kg bb
0,18 ± 0,04
0,092*
Dosis 500 mg/kg bb
0,33 ± 0,05
0,014*
Dosis 750 mg/kg bb
0,47 ± 0,05
0,004*
Dosis 1000 mg/kg bb
0,71 ± 0,08
0,024*
2. Hasil rata-rata kadar kreatinin
*) terdapat perbedaan yang signifikan
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Lampiran 11.Pengamatan makropatologi organ hati dan ginjal
Hati dan ginjal mencit kelompok kontrol
Hati dan ginjal mencit dosis 250 mg/kg bb
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Lampiran 11.(lanjutan)
Hati dan ginjal mencit dosis 500 mg/kg bb
Hati dan ginjal mencit dosis 750 mg/kg bb
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Lampiran 11.(lanjutan)
Hati dan ginjal mencit dosis 1000 mg/kg bb
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Lampiran 12. Gambar alat, bahan, dan objek yang digunakan
Oral sonde
Peralatan bedah
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Lampiran 12. (lanjutan)
Mikroskop
Preparat hati dan ginjal
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Lampiran 13. Gambar hewan percobaan yang digunakan
Mencit
7,5 cm
Pembedahan mencit
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Lampiran 14. Hasil analisis SPSS berat badan mencit jantan
Descriptives
N
Lower
Bound
Upper
Bound
Mini Maxim
mum
um
19.5434
25.4966
20.0
0
26.20
dosis 250
mg/kg bb
5
23.82
3.59889 1.6094
7
19.3514
28.2886
20.0
0
27.60
dosis 500
mg/kg bb
5
24.42
2.32099 1.0379
8
21.5381
27.3019
22.7
0
27.80
dosis 750
mg/kg bb
5
26.04
4.14101 1.8519
2
20.8983
31.1817
21.0
0
32.00
dosis
1000
mg/kg bb
5
25.34
2.02682 .90642
22.8234
27.8566
22.4
0
28.00
25
24.42
3.01780 .60356
23.1823
25.6737
32.00
5
24.00
19.9204
28.0796
5
25.14
20.2527
30.0273
5
25.70
22.5149
28.8851
5
26.00
20.8986
31.1014
5
25.26
3.28557 1.4693
5
3.93611 1.7602
8
2.56515 1.1471
7
4.10853 1.8373
9
5.70026 2.5492
4
18.1822
32.3378
20.0
0
20.0
0
20.7
0
23.6
0
20.0
0
17.0
0
25
25.22
3.76740 .75348
23.6649
26.7751
30.50
5
24.30
18.8607
29.7393
5
25.08
20.2659
29.8941
5
24.68
20.4343
28.9257
5
21.30
13.7015
28.8985
5
23.58
4.38064 1.9590
8
3.87711 1.7339
0
3.41936 1.5291
8
6.11964 2.7367
9
6.22712 2.7848
5
15.8480
31.3120
17.0
0
20.4
0
20.4
0
19.0
0
13.0
0
15.0
0
25
23.78
4.71419 .94284
21.8421
25.7339
32.30
5
24.84
20.1171
29.5629
5
25.42
20.0842
30.7558
5
25.08
20.5531
29.6069
5
22.26
3.80368 1.7010
6
4.29732 1.9218
2
3.64582 1.6304
6
6.64364 2.9711
3
14.0108
30.5092
13.0
0
21.0
0
20.4
0
18.6
0
13.0
0
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis
1000
mg/kg bb
Total
kontrol
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis
1000
mg/kg bb
Total
kontrol
berat
badan
mingg
u III
95% Confidence
Interval for Mean
2.39729 1.0721
0
Kontrol
berat
badan
mingg
u II
Std.
Error
22.52
Total
berat
badan
mingg
uI
Std.
Deviation
5
Kontrol
berat
badan
Hari 0
Mean
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
28.70
29.30
30.00
29.00
30.50
31.00
30.00
28.20
30.00
32.30
30.00
31.30
27.00
31.00
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dosis
1000
mg/kg bb
5
22.36
4.14101 1.8519
2
17.2183
27.5017
15.0
0
25.00
25
23.99
4.46318 .89264
22.1497
25.8343
31.30
5
26.16
4.13860 1.8508
4
21.0213
31.2987
13.0
0
21.8
0
dosis 250
mg/kg bb
5
25.88
3.89320 1.7410
9
21.0460
30.7140
22.0
0
31.10
dosis 500
mg/kg bb
5
25.46
4.77839 2.1369
6
19.5268
31.3932
17.0
0
28.30
dosis 750
mg/kg bb
5
22.90
7.00357 3.1320
9
14.2039
31.5961
13.0
0
32.00
dosis
1000
mg/kg bb
5
22.80
4.43847 1.9849
4
17.2889
28.3111
15.0
0
26.00
25
24.64
4.78774 .95755
22.6637
26.6163
13.0
0
32.00
Total
Kontrol
berat
badan
mingg
u Iv
Total
31.00
Test of Homogeneity of Variances
Levene Statistic
berat badan Hari 0
berat badan minggu I
berat badan minggu II
berat badan minggu III
berat badan minggu Iv
df1
1.280
1.383
.347
.481
.471
df2
4
4
4
4
4
Sig.
20
20
20
20
20
.311
.276
.843
.750
.757
ANOVA
Sum of
Squares
Between
berat badan Groups
Hari 0
Within Groups
berat badan
minggu I
berat badan
minggu II
berat badan
minggu III
Total
Between
Groups
Within Groups
Total
Between
Groups
Within Groups
Total
Between
Groups
Within Groups
Total
Between
Groups
berat badan
minggu Iv
Within Groups
Total
df
Mean
Square
F
37.202
4
9.301
181.368
20
9.068
218.570
11.676
24
4
2.919
328.964
340.640
44.802
20
24
4
16.448
488.564
533.366
48.026
20
24
4
24.428
430.052
478.078
54.668
20
24
4
21.503
495.472
20
24.774
550.140
24
11.201
12.007
13.667
Sig.
1.026
.418
.177
.947
.459
.765
.558
.695
.552
.700
92
Universitas Sumatera Utara
Post Hoc Tests
Multiple Comparisons
Tukey HSD
Dependent
Variable
(I)
perlakuan
(J)
perlakuan
kontrol
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
berat badan dosis 500
Hari 0
mg/kg bb
dosis 250
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
dosis 1000 mg/kg bb
mg/kg bb dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
kontrol
berat badan
dosis 750
minggu I
mg/kg bb
dosis 1000
mg/kg bb
dosis 250
kontrol
mg/kg bb
Mean
Difference
(I-J)
Std. Error
Sig.
-1.30000
1.90456
.958
-1.90000
1.90456
.853
-3.52000
1.90456
.375
-2.82000
1.90456
.586
1.30000
1.90456
.958
-.60000
1.90456
.998
-2.22000
1.90456
.770
-1.52000
1.90456
.928
1.90000
1.90456
.853
.60000
1.90456
.998
-1.62000
1.90456
.911
-.92000
1.90456
.988
3.52000
1.90456
.375
2.22000
1.90456
.770
1.62000
1.90456
.911
.70000
1.90456
.996
2.82000
1.90456
.586
1.52000
1.90456
.928
.92000
1.90456
.988
-.70000
1.90456
.996
-1.14000
2.56501
.991
-1.70000
2.56501
.962
-2.00000
2.56501
.934
-1.26000
2.56501
.987
1.14000
2.56501
.991
95% Confidence
Interval
Lower
Upper
Bound
Bound
4.3992
6.9992
3.7992
7.5992
2.1792
9.2192
2.8792
8.5192
6.9992
4.3992
5.0992
6.2992
3.4792
7.9192
4.1792
7.2192
7.5992
3.7992
6.2992
5.0992
4.0792
7.3192
4.7792
6.6192
9.2192
2.1792
7.9192
3.4792
7.3192
4.0792
6.3992
4.9992
8.5192
2.8792
7.2192
4.1792
6.6192
4.7792
4.9992
6.3992
6.5355
8.8155
5.9755
9.3755
5.6755
9.6755
6.4155
8.9355
8.8155
6.5355
93
Universitas Sumatera Utara
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 500
mg/kg bb
dosis 250
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
dosis 1000 mg/kg bb
mg/kg bb dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 250
mg/kg bb
kontrol
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
mg/kg bb
berat badan
minggu II
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 500
mg/kg bb
dosis 250
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
-.56000
2.56501
.999
-.86000
2.56501
.997
-.12000
2.56501
1.000
1.70000
2.56501
.962
.56000
2.56501
.999
-.30000
2.56501
1.000
.44000
2.56501
1.000
2.00000
2.56501
.934
.86000
2.56501
.997
.30000
2.56501
1.000
.74000
2.56501
.998
1.26000
2.56501
.987
.12000
2.56501
1.000
-.44000
2.56501
1.000
-.74000
2.56501
.998
-.78000
3.12590
.999
-.38000
3.12590
1.000
3.00000
3.12590
.870
.72000
3.12590
.999
.78000
3.12590
.999
.40000
3.12590
1.000
3.78000
3.12590
.746
1.50000
3.12590
.988
.38000
3.12590
1.000
-.40000
3.12590
1.000
3.38000
3.12590
.814
1.10000
3.12590
.996
-3.00000
3.12590
.870
-3.78000
3.12590
.746
-3.38000
3.12590
.814
kontrol
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
8.2355
8.5355
7.7955
5.9755
7.1155
7.9755
7.2355
5.6755
6.8155
7.3755
6.9355
6.4155
7.5555
8.1155
8.4155
10.133
9
9.7339
6.3539
8.6339
8.5739
8.9539
5.5739
7.8539
8.9739
9.7539
5.9739
8.2539
12.353
9
13.133
9
12.733
9
7.1155
6.8155
7.5555
9.3755
8.2355
7.3755
8.1155
9.6755
8.5355
7.9755
8.4155
8.9355
7.7955
7.2355
6.9355
8.5739
8.9739
12.3539
10.0739
10.1339
9.7539
13.1339
10.8539
9.7339
8.9539
12.7339
10.4539
6.3539
5.5739
5.9739
94
Universitas Sumatera Utara
dosis 1000
mg/kg bb
-2.28000
3.12590
.947
-.72000
3.12590
.999
-1.50000
3.12590
.988
-1.10000
3.12590
.996
2.28000
3.12590
.947
-.58000
2.93275
1.000
-.24000
2.93275
1.000
2.58000
2.93275
.901
2.48000
2.93275
.913
.58000
2.93275
1.000
.34000
2.93275
1.000
3.16000
2.93275
.816
3.06000
2.93275
.832
.24000
2.93275
1.000
-.34000
2.93275
1.000
2.82000
2.93275
.869
2.72000
2.93275
.883
-2.58000
2.93275
.901
-3.16000
2.93275
.816
-2.82000
2.93275
.869
-.10000
2.93275
1.000
-2.48000
2.93275
.913
-3.06000
2.93275
.832
-2.72000
2.93275
.883
.10000
2.93275
1.000
.28000
3.14793
1.000
.70000
3.14793
.999
kontrol
dosis 250
dosis 1000 mg/kg bb
mg/kg bb
dosis 500
mg/kg bb
kontrol
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 500
mg/kg bb
berat badan
minggu III
dosis 250
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
dosis 1000 mg/kg bb
mg/kg bb
dosis 500
mg/kg bb
berat badan
kontrol
minggu Iv
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
11.633
9
10.073
9
10.853
9
10.453
9
7.0739
9.3559
9.0159
6.1959
6.2959
8.1959
8.4359
5.6159
5.7159
8.5359
9.1159
5.9559
6.0559
11.355
9
11.935
9
11.595
9
8.8759
11.255
9
11.835
9
11.495
9
8.6759
9.1398
8.7198
7.0739
8.6339
7.8539
8.2539
11.6339
8.1959
8.5359
11.3559
11.2559
9.3559
9.1159
11.9359
11.8359
9.0159
8.4359
11.5959
11.4959
6.1959
5.6159
5.9559
8.6759
6.2959
5.7159
6.0559
8.8759
9.6998
10.1198
95
Universitas Sumatera Utara
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
3.26000
3.14793
.836
3.36000
3.14793
.821
-.28000
3.14793
1.000
.42000
3.14793
1.000
2.98000
3.14793
.875
3.08000
3.14793
.862
-.70000
3.14793
.999
-.42000
3.14793
1.000
2.56000
3.14793
.923
2.66000
3.14793
.913
-3.26000
3.14793
.836
-2.98000
3.14793
.875
-2.56000
3.14793
.923
.10000
3.14793
1.000
-3.36000
3.14793
.821
-3.08000
3.14793
.862
-2.66000
3.14793
.913
-.10000
3.14793
1.000
kontrol
dosis 500
mg/kg bb
dosis 250
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
dosis 1000 mg/kg bb
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
6.1598
6.0598
9.6998
8.9998
6.4398
6.3398
10.119
8
9.8398
6.8598
6.7598
12.679
8
12.399
8
11.979
8
9.3198
12.779
8
12.499
8
12.079
8
9.5198
12.6798
12.7798
9.1398
9.8398
12.3998
12.4998
8.7198
8.9998
11.9798
12.0798
6.1598
6.4398
6.8598
9.5198
6.0598
6.3398
6.7598
9.3198
96
Universitas Sumatera Utara
Lampiran 15. Hasil analisis SPSS pakan mencit
Oneway
Descriptives
Jumlah pakan(Gram)
95% Confidence
Interval for Mean
Std.
N
Mean
Deviation
Std. Error
Lower
Upper
Minim
Maximu
Bound
Bound
um
m
kontrol
14
38,7929
2,33517
,62410
37,4446
40,1411
35,60
43,20
250 mg
14
39,9857
2,50564
,66966
38,5390
41,4324
35,40
43,20
500 mg
14
36,9571
3,66054
,97832
34,8436
39,0707
31,00
P42,00
750 mg
14
28,9714
7,88059
2,10618
24,4213
33,5215
20,50
46,10
1000 mg
14
24,1286
9,61733
2,57034
18,5757
29,6815
14,80
44,80
Total
70
33,7671
8,50961
1,01709
31,7381
35,7962
14,80
46,10
ANOVA
Jumlah pakan(Gram)
Sum of Squares
df
Mean Square
F
Between Groups
2660,077
4
665,019
Within Groups
2336,458
65
35,946
Total
4996,534
69
Sig.
18,501
,000
Post Hoc Tests
Multiple Comparisons
Dependent Variable: Jumlah pakan(Gram)
(I)
(J)
Perlakua Perlaku
Tukey HSD
95% Confidence Interval
Mean
Difference
Std.
(I-J)
Error
Lower
n
an
kontrol
250 mg
-1,19286
2,26607
,984
-7,5511
5,1653
500 mg
1,83571
2,26607
,927
-4,5225
8,1939
750 mg
9,82143
*
2,26607
,000
3,4632
16,1796
*
2,26607
,000
8,3061
21,0225
kontrol
1,19286
2,26607
,984
-5,1653
7,5511
500 mg
3,02857
2,26607
,670
-3,3296
9,3868
750 mg
11,01429
*
2,26607
,000
4,6561
17,3725
15,85714
*
2,26607
,000
9,4989
22,2153
kontrol
-1,83571
2,26607
,927
-8,1939
4,5225
250 mg
-3,02857
2,26607
,670
-9,3868
3,3296
1000 mg
250 mg
1000 mg
500 mg
14,66429
Sig.
Bound
Upper Bound
97
Universitas Sumatera Utara
*
2,26607
,007
1,6275
14,3439
*
2,26607
,000
6,4704
19,1868
*
2,26607
,000
-16,1796
-3,4632
*
2,26607
,000
-17,3725
-4,6561
*
2,26607
,007
-14,3439
-1,6275
4,84286
2,26607
,217
-1,5153
11,2011
-14,66429
*
2,26607
,000
-21,0225
-8,3061
250 mg
-15,85714
*
2,26607
,000
-22,2153
-9,4989
500 mg
-12,82857
*
2,26607
,000
-19,1868
-6,4704
750 mg
-4,84286
2,26607
,217
-11,2011
1,5153
250 mg
-1,19286
2,26607
,600
-5,7185
3,3328
500 mg
1,83571
2,26607
,421
-2,6899
6,3614
750 mg
*
2,26607
,000
5,2958
14,3471
*
2,26607
,000
10,1386
19,1899
kontrol
1,19286
2,26607
,600
-3,3328
5,7185
500 mg
3,02857
2,26607
,186
-1,4971
7,5542
750 mg
11,01429
*
2,26607
,000
6,4886
15,5399
15,85714
*
2,26607
,000
11,3315
20,3828
kontrol
-1,83571
2,26607
,421
-6,3614
2,6899
250 mg
-3,02857
2,26607
,186
-7,5542
1,4971
750 mg
*
2,26607
,001
3,4601
12,5114
*
2,26607
,000
8,3029
17,3542
*
2,26607
,000
-14,3471
-5,2958
*
2,26607
,000
-15,5399
-6,4886
-7,98571
*
2,26607
,001
-12,5114
-3,4601
4,84286
*
2,26607
,036
,3172
9,3685
-14,66429
*
2,26607
,000
-19,1899
-10,1386
-15,85714
*
2,26607
,000
-20,3828
-11,3315
-12,82857
*
2,26607
,000
-17,3542
-8,3029
*
2,26607
,036
-9,3685
-,3172
750 mg
750 mg
1000 mg
12,82857
kontrol
-9,82143
250 mg
-11,01429
500 mg
-7,98571
1000 mg
1000 mg kontrol
LSD
kontrol
1000 mg
250 mg
1000 mg
500 mg
1000 mg
750 mg
7,98571
kontrol
250 mg
500 mg
1000 mg
1000 mg kontrol
250 mg
500 mg
750 mg
9,82143
14,66429
7,98571
12,82857
-9,82143
-11,01429
-4,84286
*. The mean difference is significant at the 0.05 level.
98
Universitas Sumatera Utara
Lampiran 16. Hasil analisis SPSS kadar ALT dan kreatinin
Descriptives
95% Confidence Interval for
N
Mean
Std.
Std.
Deviation
Error
Mean
Lower Bound
Upper Bound
Min
Max
kadar
kontrol
5
24,2600
7,67222 3,43112
14,7337
33,7863
11,90
31,60
ALT
250 mg
5
48,5000
7,00321 3,13193
39,8044
57,1956
36,60
55,10
500 mg
4
73,5000
19,46244 9,73122
42,5309
104,4691
55,80
92,00
750 mg
3
99,5000
12,99038 7,50000
67,2301
131,7699
92,00
114,50
2
151,1000
153,6412 150,90
151,30
19
66,2368
1000
,28284
,20000
148,5588
40,97605 9,40055
46,4870
85,9867
11,90
151,30
mg
Total
kadar
kontrol
5
,1200
,04637
,02074
,0624
,1776
,05
,17
kreati
250 mg
5
,1840
,04450
,01990
,1287
,2393
,13
,23
500 mg
4
,3325
,05252
,02626
,2489
,4161
,30
,41
750 mg
3
,4667
,04933
,02848
,3441
,5892
,41
,50
2
,7100
,08485
,06000
-,0524
1,4724
,65
,77
19
,2984
,19577
,04491
,2041
,3928
,05
,77
nin
1000
mg
Total
Tests of Normality
a
Kolmogorov-Smirnov
Perlakuan
kadar
kreatinin
kadar ALT
Statistic
kontrol
df
,267
Shapiro-Wilk
Sig.
5
Statistic
df
Sig.
,200
*
,930
5
,593
*
,877
5
,298
250 mg
,249
5
,200
500 mg
,344
4
.
,753
4
,041
750 mg
,349
3
.
,832
3
,194
1000 mg
,260
2
.
kontrol
,249
5
,200
*
,898
5
,399
250 mg
,362
5
,031
,823
5
,123
500 mg
,293
4
.
,794
4
,092
750 mg
,385
3
.
,750
3
,000
1000 mg
,260
2
.
*. This is a lower bound of the true significance.
a. Lilliefors Significance Correction
99
Universitas Sumatera Utara
Lampiran 16.(lanjutan)
NPAR TESTS
/K-W=kadar kdr BY perlakuan(1 5)
/MISSING ANALYSIS.
NPar Tests
Kruskal-Wallis Test
Ranks
Perlakuan
kadar ALT
N
Kontrol
5
3,00
250 mg
5
8,00
500 mg
4
12,75
750 mg
3
15,67
1000 mg
2
18,50
Total
kadar kreatinin
Mean Rank
19
Kontrol
5
3,90
250 mg
5
7,10
500 mg
4
12,63
750 mg
3
15,83
1000 mg
2
18,50
Total
19
a,b
Test Statistics
kadar ALT
Chi-Square
16,989
15,930
4
4
,002
,003
Df
Asymp. Sig.
kadar kreatinin
a. Kruskal Wallis Test
b. Grouping Variable: Perlakuan
100
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NPAR TESTS
/M-W= kadar kdr BY perlakuan(1 2)
/MISSING ANALYSIS.
NPar Tests
Mann-Whitney Test
Ranks
Perlakuan
kadar ALT
N
Mean Rank
kontrol
5
3,00
15,00
250 mg
5
8,00
40,00
Total
kadar kreatinin
Sum of Ranks
10
kontrol
5
3,90
19,50
250 mg
5
7,10
35,50
Total
10
a
Test Statistics
kadar ALT
Mann-Whitney U
kadar kreatinin
,000
4,500
Wilcoxon W
15,000
19,500
Z
-2,611
-1,687
,009
,092
Asymp. Sig. (2-tailed)
Exact Sig. [2*(1-tailed Sig.)]
,008
b
,095
b
a. Grouping Variable: Perlakuan
b. Not corrected for ties.
NPAR TESTS
/M-W= kadar kdr BY perlakuan(1 3)
/MISSING ANALYSIS.
NPar Tests
Mann-Whitney Test
Ranks
Perlakuan
kadar ALT
kadar kreatinin
N
Mean Rank
Sum of Ranks
kontrol
5
3,00
15,00
500 mg
4
7,50
30,00
Total
9
kontrol
5
3,00
15,00
500 mg
4
7,50
30,00
Total
9
101
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a
Test Statistics
kadar ALT
Mann-Whitney U
kadar kreatinin
,000
,000
Wilcoxon W
15,000
15,000
Z
-2,449
-2,470
,014
,014
Asymp. Sig. (2-tailed)
Exact Sig. [2*(1-tailed Sig.)]
,016
b
,016
b
a. Grouping Variable: Perlakuan
b. Not corrected for ties.
NPAR TESTS
/K-W=kadar kdr BY perlakuan(1 4)
/STATISTICS DESCRIPTIVES
/MISSING ANALYSIS.
NPar Tests
Kruskal-Wallis Test
Ranks
Perlakuan
kadar ALT
N
Kontrol
5
3,00
250 mg
5
8,00
500 mg
4
12,75
750 mg
3
15,67
Total
kadar kreatinin
Mean Rank
17
Kontrol
5
3,90
250 mg
5
7,10
500 mg
4
12,63
750 mg
3
15,83
Total
17
a,b
Test Statistics
kadar ALT
Chi-Square
14,762
13,445
3
3
,002
,004
Df
Asymp. Sig.
kadar kreatinin
a. Kruskal Wallis Test
b. Grouping Variable: Perlakuan
102
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NPAR TESTS
/M-W= kadar kdr BY perlakuan(1 5)
/MISSING ANALYSIS.
NPar Tests
Mann-Whitney Test
Ranks
Perlakuan
kadar ALT
kadar kreatinin
N
Mean Rank
Sum of Ranks
Kontrol
5
3,00
15,00
1000 mg
3
7,00
21,00
Total
8
Kontrol
5
3,00
15,00
1000 mg
3
7,00
21,00
Total
8
a
Test Statistics
kadar ALT
Mann-Whitney U
kadar kreatinin
,000
,000
Wilcoxon W
15,000
15,000
Z
-2,249
-2,263
,024
,024
Asymp. Sig. (2-tailed)
Exact Sig. [2*(1-tailed Sig.)]
,036
b
,036
b
a. Grouping Variable: Perlakuan
b. Not corrected for ties.
103
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56
Universitas Sumatera Utara
Lampiran 2. Gambar tumbuhan dan daun segarkembang bulan (Tithonia
diversifolia (Hemsley) A. Gray)
Tumbuhan kembang bulan
Daun segar tumbuhan kembang bulan
57
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Lampiran 3.GambarSimplisia dan serbuk simplisia daun kembang bulan
Simplisia daun kembang bulan
Serbuk simplisia daun kembang bulan
58
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Lampiran 4. Bagan pembuatan ekstrak
Serbuk simplisia
Dimasukkan ke dalam bejana
Ditambah pelarut 5,5 liter etanol 80% (75
bagian)
Dibiarkan selama 5 hari terlindung dari
cahaya sambil sesekali di aduk
Diserkai, diperas
Maserat I
Ampas
Dicuci dengan pelarut
etanol 80% hingga
diperoleh (100 bagian)
Dipindahkan ke dalam
bejana tertutup
Dibiarkan di tempat
sejuk, terlindung dari
cahaya selama 2 hari
Dienap tuangkan dan
di saring
Maserat II
Ampas
Digabung
Dipekatkan dengan alat rotary evaporator 40oC
Dikeringkan dengan alat freeze dryer -40oC
Ekstrak Kental
(71 g)
59
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Lampiran 5. Bagan alur penelitian uji toksisitas subkronik
EEDKB
`
Hewan Uji
(Mencit)
Kelompok
kontrol (I)
Kelompok
II
Kelompok
III
Kelompok
IV
Kelompok
IV
Diberi
CMC Na
5%
Diberi
EEDKB dosis
250 mg/kg bb
Diberi
EEDKB dosis
500 mg/kg bb
Diberi
EEDKB dosis
750 mg/kg bb
Diberi
EEDKB dosis
1000 mg/kg
bb
Pengamatan 28 Hari
Gejala
toksik
Berat
Badan
Jumlah
pakan
Kematian
Kadar
ALT dan
Kreatinin
Makropa
-tologi
hati dan
ginjal
Histopatologi
hati dan
ginjal
Analisis data
60
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Lampiran 6. Contoh perhitungan dosis
Berat badan mencit 25 g dengan dosis EEDKB 1000 mg/kg bb
Dosis = 1000 mg/kg bb
= (25 : 1000 kg ) x 1000 mg/kg
= 25 mg
Konsentrasi ekstrak yang dibuat 6% = 6 g/100 mL
= 6000 mg/100 mL
= 60 mg/mL
Jumlah obat yang disuntikkan
= 25 mg : 60 mg/mL
= 0,42 mL
61
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Lampiran 7. Hasil uji
pendahuluan1. Hasil pengamatan
berat badan
Perlakuan
Kontrol I
Kontrol II
Dosis 250 mg I
Dosis 250 mg II
Dosis 500 mg I
Dosis 500 mg II
Dosis 1000 mg I
Dosis 1000 mg II
Dosis 1500 mg I
Dosis 1500 mg II
Dosis 3000 mg I
Dosis 3000 mg II
0
27,1
26,0
27
26,5
24
25,1
23,5
25,8
24,2
25,2
23
26,3
1
27,2
26,1
27,1
26,5
24
25
23,5
26
24
25
23
26
Berat badan hari ke- (g)
2
3
4
5
26,8 27,0 26,9 27,2
25,9 26,0 26,1 26,2
26,9 26,8 26,8 26,9
26,4 26,7 26,8 27
23,8
24 23,9 23,5
25,1 24,7 25 24,8
24
23,7 23,5 22
25,7 25,4 24,6 25
23
22,2 21
19
24
24,4 24,5 24,2
22,4 22,1 20
18
18
18
18
18
6
7
27,5 27,3
26,5 26,6
27,1 27,3
26,9 26,8
23,6 23,8
25 25,2
22
22
25,1 25,2
Mati
24,1 24
Mati
Mati
2. Hasil perhitungan dan analisa rata-rata berat badan
Hari
0
7
Rata-rata berat badan (g) ± SD
kontrol
26,55 ±0,78
250 mg
26,75±0,35
500 mg
24,55±0,78
1000 mg
24,65±1,63
1500 mg
24,7±0,71
3000 mg
24,65±2,33
26,95±0,49
27,05±0,35
24,5±0,99
23,6±2,26
24,00±
-
3. Hasil pengamatan gejala toksik
Perubahan bulu
Gerak gerik
Jalan
dengan Gelisah
perut
Rontok
Warna
Jalan
mundur
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
+
-
-
+
-
-
-
-
+
+
-
-
+
-
-
-
-
+
Perlakuan
Kejang
Salivasi
Diare
Lemas
Kontrol
Dosis
250 mg
Dosis
500 mg
Dosis
1000 g
Dosis
1500 mg
Dosis
3000 mg
-
-
-
-
-
-
62
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Lampiran 8. Hasil pengukuran berat badan
1. Berat badan pada minggu pertama
Perlakuan
kontrol I
kontrol II
kontrol III
kontrol IV
kontrol V
Dosis 250 mg I
Dosis 250 mg II
Dosis 250 mg III
Dosis 250 mg IV
Dosis 250 mg V
Dosis 500 mg I
Dosis 500 mg II
Dosis 500 mg III
Dosis 500 mg IV
Dosis 500 mg V
Dosis 750 mg I
Dosis 750 mg II
Dosis 750 mg III
Dosis 750 mg IV
Dosis 750 mg V
Dosis 1000 mg I
Dosis 1000 mg II
Dosis 1000 mg III
Dosis 1000 mg IV
Dosis 1000 mg V
1
23,3
20
21
22,1
26,2
20,2
27,6
24,4
20
26,9
27,8
22,7
23
25,9
22,7
32
27,8
21
25,4
24
25,2
28
26,1
22,4
25
2
24,9
20,1
21
24,1
27,8
22
28
26
20,1
29
30
24,5
25
26,8
25
32,2
30
22,3
27,8
23,4
25
28,8
28,7
21
26,6
Berat badan hari ke- (g)
3
4
5
23,3
23,5
22
20,2
20,1
20
21,2
21,2
21,1
24
25,1
25
28
27,8
28,4
21.5
20,3
21,6
28,5
28,7
29
25,5
25,6
24,6
20,2
20
20,5
28,8
28,9
29
29,8
28,9
31
24
23,8
23,9
24,8
23,9
25
25,8
24,9
25
23,8
22,9
23,5
31,6
31,7
33
30
28,3
29
25
24
23
28
28,9
29
22
22,8
23,7
24,3
22
19
28,9
29,2
30
29
28,9
29
20
20
21
26
25,6
25,7
6
23,5
20
21,5
24
29
21,8
29,1
25
21
28,8
29,8
24
24,7
26,1
23,4
32
28,8
19,5
29
23,5
18,8
31
30
22,2
26
7
24,3
20
22
25
28,7
22
29,3
24,7
20,7
29
30
24
25
25,9
23,6
29
29
20
28,6
23,4
17
30,5
30,3
22,4
26,1
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2. Berat badan pada minggu kedua
Perlakuan
kontrol I
kontrol II
kontrol III
kontrol IV
kontrol V
Dosis 250 mg I
Dosis 250 mg II
Dosis 250 mg III
Dosis 250 mg IV
Dosis 250 mg V
Dosis 500 mg I
Dosis 500 mg II
Dosis 500 mg III
Dosis 500 mg IV
Dosis 500 mg V
Dosis 750 mg I
Dosis 750 mg II
Dosis 750 mg III
Dosis 750 mg IV
Dosis 750 mg V
Dosis 1000 mg I
Dosis 1000 mg II
Dosis 1000 mg III
Dosis 1000 mg IV
Dosis 1000 mg V
1
24
20
21,4
25
29
21
29,6
26
21
28,7
28,4
24
25
26
24
25
30
20
27,6
20
16
30,6
30
22,4
26
2
24
20
21,3
24,5
29
21
30
25,4
20,8
28,5
28,1
22,3
24,5
25
23,8
24
30,2
21,4
24,5
18
15
30,7
30,6
22,1
25,8
Berat badan hari ke- (g)
3
4
5
6
7
23,7
24
23,8
23,6
23,4
20,2
20,1
20,2
20,3
20,7
21,3
21,8
21,4
20,3
20,4
25
24,8
24,9
25,7
26
30
30,3
30,4
30,6
31
20
20
20,5
20,4
20,4
30
29,7
29,5
28,8
30
25,4
25,2
24
23,8
26
21
22
21
21,3
22
28,3
27,8
26,9
27
27
26,8
27
25,8
28
28,2
20
19,4
16,5
18
19
23,6
24
23,7
25
25,2
23,8
23,6
23
24,4
25
24
24,6
24,7
25,3
26
21
20
Mati
29,5
29,6
28,4
30
30,2
21
20,6
21,8
20
21,4
23
25,2
20
27,6
24,5
17
18
16
20
18
15
Mati
31,4
32
30,6
30,7
31,4
31
30
24
Mati
21,6
22,4
22,1
21,6
22
25,6
26
26
25,8
25,6
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3. Berat badan pada minggu ketiga
Perlakuan
kontrol I
kontrol II
kontrol III
kontrol IV
kontrol V
Dosis 250 mg I
Dosis 250 mg II
Dosis 250 mg III
Dosis 250 mg IV
Dosis 250 mg V
Dosis 500 mg I
Dosis 500 mg II
Dosis 500 mg III
Dosis 500 mg IV
Dosis 500 mg V
Dosis 750 mg I
Dosis 750 mg II
Dosis 750 mg III
Dosis 750 mg IV
Dosis 750 mg V
Dosis 1000 mg I
Dosis 1000 mg II
Dosis 1000 mg III
Dosis 1000 mg IV
Dosis 1000 mg V
1
23,6
20,5
20,3
26
31
20,3
30,8
26,4
21,9
27
28,1
19
25
25
26
2
24,3
20,6
20,4
26
31,6
20,4
31,7
26,8
21,8
27,1
27,7
18
25,3
23,8
26
30
23
20,5
13
31
24,5
20,2
32
24
21,9
24,6
22,5
24,7
Berat badan hari ke- (g)
3
4
5
25
24,7
25
20,4
20,8
21
20,2
20,8
20,8
25,8
25,5
26
31,8
32
31,7
20,5
20
20,3
32
31,6
30,5
27
26,7
26,5
21,9
22
21,8
26,7
26,4
26,4
27,6
27,4
27,2
18
18
19
25,7
26
26,5
24,7
25,7
25,6
26,2
26.7
27
Mati
31
29,8
29,9
24,8
23,9
25
20
19
20
Mati
Mati
Mati
Mati
23
22,8
23,6
24,6
25
24,7
6
25
21,3
21
26,4
31,5
20,5
31
26,6
22
26,2
27
19
27
25,8
26,8
7
24,8
21,4
21
27
30
20,4
31,3
26,8
22,1
26,5
27
18,6
26,8
26
27
30
25,1
21,4
31
25,3
22
24
24,3
23,8
25
65
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4. Berat badan pada minggu keempat
Perlakuan
kontrol I
kontrol II
kontrol III
kontrol IV
kontrol V
Dosis 250 mg I
Dosis 250 mg II
Dosis 250 mg III
Dosis 250 mg IV
Dosis 250 mg V
Dosis 500 mg I
Dosis 500 mg II
Dosis 500 mg III
Dosis 500 mg IV
Dosis 500 mg V
Dosis 750 mg I
Dosis 750 mg II
Dosis 750 mg III
Dosis 750 mg IV
Dosis 750 mg V
Dosis 1000 mg I
Dosis 1000 mg II
Dosis 1000 mg III
Dosis 1000 mg IV
Dosis 1000 mg V
1
22
25
21,7
21
27
20,7
31
26,4
21,3
27
26,4
20
27,3
26
27,5
2
23
25,5
22
20,7
27,7
21
30,6
27
21
26,8
26
19
29
25,5
28
30,8
25,1
22,5
31
25
23,1
23,6
25
24
25,6
Berat badan hari ke- (g)
3
4
5
6
24
25
26
27
25,8
25,8
26
26,6
21,8
21,6
21,6
21,9
20,8
21
21,3
21,6
28
28
28
29
21,2
21,6
21,5
21,8
30,8
31
30,4
30,7
26
27,1
26,5
26,7
21,7
21,8
21,6
22
25,8
26,4
27
26,5
25,7
26,9
26
26,4
17
17
Mati
28,6
28,8
29
28,5
25
26
27
27,6
26,9
26,5
27
27,2
Mati
30,7
30,9
31,5
31,7
25,4
26
25,3
25,6
23
23,6
25
24,7
Mati
Mati
Mati
Mati
23,7
23,8
24
24,7
25,8
26
26,2
26
7
28
27
22
21,8
29
22
31,1
27,3
22
27
26,5
28,3
28
27,5
32
25
24,5
25
26
66
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Lampiran 9. Hasil pengukuran kadar ALT dan kreatinin
67
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Lampiran 9. (lanjutan)
68
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Lampiran 9.(lanjutan)
69
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Lampiran 9.(lanjutan)
70
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Lampiran 9.(lanjutan)
71
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Lampiran 9.(lanjutan)
72
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Lampiran 9. (lanjutan)
73
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Lampiran 9.(lanjutan)
74
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Lampiran 9.(lanjutan)
75
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Lampiran 9.(lanjutan)
76
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Lampiran 9.(lanjutan)
77
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Lampiran 9.(lanjutan)
78
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Lampiran 9.(lanjutan)
79
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Lampiran 9.(lanjutan)
80
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Lampiran 9.(lanjutan)
81
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Lampiran 9.(lanjutan)
82
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Lampiran 9.(lanjutan)
83
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Lampiran 10. Hasil rata-rata kadar ALT dan kreatinin
1. Hasil rata-rata kadar ALT
Perlakuan
Rata-Rata ±SD
Nilai signifikansi
Kontrol
24,26 ±7,67
-
Dosis 250 mg/kg bb
48,5 ± 7,00
0,09*
Dosis 500 mg/kg bb
73,5 ± 19,46
0,014*
Dosis 750 mg/kg bb
99,5 ± 12,99
0,002*
Dosis 1000 mg/kg bb
151,2 ± 0,80
0,024*
Perlakuan
Rata-Rata ±SD
Nilai signifikansi
Kontrol
0,12 ± 0,05
-
Dosis 250 mg/kg bb
0,18 ± 0,04
0,092*
Dosis 500 mg/kg bb
0,33 ± 0,05
0,014*
Dosis 750 mg/kg bb
0,47 ± 0,05
0,004*
Dosis 1000 mg/kg bb
0,71 ± 0,08
0,024*
2. Hasil rata-rata kadar kreatinin
*) terdapat perbedaan yang signifikan
84
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Lampiran 11.Pengamatan makropatologi organ hati dan ginjal
Hati dan ginjal mencit kelompok kontrol
Hati dan ginjal mencit dosis 250 mg/kg bb
85
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Lampiran 11.(lanjutan)
Hati dan ginjal mencit dosis 500 mg/kg bb
Hati dan ginjal mencit dosis 750 mg/kg bb
86
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Lampiran 11.(lanjutan)
Hati dan ginjal mencit dosis 1000 mg/kg bb
87
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Lampiran 12. Gambar alat, bahan, dan objek yang digunakan
Oral sonde
Peralatan bedah
88
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Lampiran 12. (lanjutan)
Mikroskop
Preparat hati dan ginjal
89
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Lampiran 13. Gambar hewan percobaan yang digunakan
Mencit
7,5 cm
Pembedahan mencit
90
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Lampiran 14. Hasil analisis SPSS berat badan mencit jantan
Descriptives
N
Lower
Bound
Upper
Bound
Mini Maxim
mum
um
19.5434
25.4966
20.0
0
26.20
dosis 250
mg/kg bb
5
23.82
3.59889 1.6094
7
19.3514
28.2886
20.0
0
27.60
dosis 500
mg/kg bb
5
24.42
2.32099 1.0379
8
21.5381
27.3019
22.7
0
27.80
dosis 750
mg/kg bb
5
26.04
4.14101 1.8519
2
20.8983
31.1817
21.0
0
32.00
dosis
1000
mg/kg bb
5
25.34
2.02682 .90642
22.8234
27.8566
22.4
0
28.00
25
24.42
3.01780 .60356
23.1823
25.6737
32.00
5
24.00
19.9204
28.0796
5
25.14
20.2527
30.0273
5
25.70
22.5149
28.8851
5
26.00
20.8986
31.1014
5
25.26
3.28557 1.4693
5
3.93611 1.7602
8
2.56515 1.1471
7
4.10853 1.8373
9
5.70026 2.5492
4
18.1822
32.3378
20.0
0
20.0
0
20.7
0
23.6
0
20.0
0
17.0
0
25
25.22
3.76740 .75348
23.6649
26.7751
30.50
5
24.30
18.8607
29.7393
5
25.08
20.2659
29.8941
5
24.68
20.4343
28.9257
5
21.30
13.7015
28.8985
5
23.58
4.38064 1.9590
8
3.87711 1.7339
0
3.41936 1.5291
8
6.11964 2.7367
9
6.22712 2.7848
5
15.8480
31.3120
17.0
0
20.4
0
20.4
0
19.0
0
13.0
0
15.0
0
25
23.78
4.71419 .94284
21.8421
25.7339
32.30
5
24.84
20.1171
29.5629
5
25.42
20.0842
30.7558
5
25.08
20.5531
29.6069
5
22.26
3.80368 1.7010
6
4.29732 1.9218
2
3.64582 1.6304
6
6.64364 2.9711
3
14.0108
30.5092
13.0
0
21.0
0
20.4
0
18.6
0
13.0
0
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis
1000
mg/kg bb
Total
kontrol
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis
1000
mg/kg bb
Total
kontrol
berat
badan
mingg
u III
95% Confidence
Interval for Mean
2.39729 1.0721
0
Kontrol
berat
badan
mingg
u II
Std.
Error
22.52
Total
berat
badan
mingg
uI
Std.
Deviation
5
Kontrol
berat
badan
Hari 0
Mean
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
28.70
29.30
30.00
29.00
30.50
31.00
30.00
28.20
30.00
32.30
30.00
31.30
27.00
31.00
91
Universitas Sumatera Utara
dosis
1000
mg/kg bb
5
22.36
4.14101 1.8519
2
17.2183
27.5017
15.0
0
25.00
25
23.99
4.46318 .89264
22.1497
25.8343
31.30
5
26.16
4.13860 1.8508
4
21.0213
31.2987
13.0
0
21.8
0
dosis 250
mg/kg bb
5
25.88
3.89320 1.7410
9
21.0460
30.7140
22.0
0
31.10
dosis 500
mg/kg bb
5
25.46
4.77839 2.1369
6
19.5268
31.3932
17.0
0
28.30
dosis 750
mg/kg bb
5
22.90
7.00357 3.1320
9
14.2039
31.5961
13.0
0
32.00
dosis
1000
mg/kg bb
5
22.80
4.43847 1.9849
4
17.2889
28.3111
15.0
0
26.00
25
24.64
4.78774 .95755
22.6637
26.6163
13.0
0
32.00
Total
Kontrol
berat
badan
mingg
u Iv
Total
31.00
Test of Homogeneity of Variances
Levene Statistic
berat badan Hari 0
berat badan minggu I
berat badan minggu II
berat badan minggu III
berat badan minggu Iv
df1
1.280
1.383
.347
.481
.471
df2
4
4
4
4
4
Sig.
20
20
20
20
20
.311
.276
.843
.750
.757
ANOVA
Sum of
Squares
Between
berat badan Groups
Hari 0
Within Groups
berat badan
minggu I
berat badan
minggu II
berat badan
minggu III
Total
Between
Groups
Within Groups
Total
Between
Groups
Within Groups
Total
Between
Groups
Within Groups
Total
Between
Groups
berat badan
minggu Iv
Within Groups
Total
df
Mean
Square
F
37.202
4
9.301
181.368
20
9.068
218.570
11.676
24
4
2.919
328.964
340.640
44.802
20
24
4
16.448
488.564
533.366
48.026
20
24
4
24.428
430.052
478.078
54.668
20
24
4
21.503
495.472
20
24.774
550.140
24
11.201
12.007
13.667
Sig.
1.026
.418
.177
.947
.459
.765
.558
.695
.552
.700
92
Universitas Sumatera Utara
Post Hoc Tests
Multiple Comparisons
Tukey HSD
Dependent
Variable
(I)
perlakuan
(J)
perlakuan
kontrol
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
berat badan dosis 500
Hari 0
mg/kg bb
dosis 250
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
dosis 1000 mg/kg bb
mg/kg bb dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
kontrol
berat badan
dosis 750
minggu I
mg/kg bb
dosis 1000
mg/kg bb
dosis 250
kontrol
mg/kg bb
Mean
Difference
(I-J)
Std. Error
Sig.
-1.30000
1.90456
.958
-1.90000
1.90456
.853
-3.52000
1.90456
.375
-2.82000
1.90456
.586
1.30000
1.90456
.958
-.60000
1.90456
.998
-2.22000
1.90456
.770
-1.52000
1.90456
.928
1.90000
1.90456
.853
.60000
1.90456
.998
-1.62000
1.90456
.911
-.92000
1.90456
.988
3.52000
1.90456
.375
2.22000
1.90456
.770
1.62000
1.90456
.911
.70000
1.90456
.996
2.82000
1.90456
.586
1.52000
1.90456
.928
.92000
1.90456
.988
-.70000
1.90456
.996
-1.14000
2.56501
.991
-1.70000
2.56501
.962
-2.00000
2.56501
.934
-1.26000
2.56501
.987
1.14000
2.56501
.991
95% Confidence
Interval
Lower
Upper
Bound
Bound
4.3992
6.9992
3.7992
7.5992
2.1792
9.2192
2.8792
8.5192
6.9992
4.3992
5.0992
6.2992
3.4792
7.9192
4.1792
7.2192
7.5992
3.7992
6.2992
5.0992
4.0792
7.3192
4.7792
6.6192
9.2192
2.1792
7.9192
3.4792
7.3192
4.0792
6.3992
4.9992
8.5192
2.8792
7.2192
4.1792
6.6192
4.7792
4.9992
6.3992
6.5355
8.8155
5.9755
9.3755
5.6755
9.6755
6.4155
8.9355
8.8155
6.5355
93
Universitas Sumatera Utara
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 500
mg/kg bb
dosis 250
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
dosis 1000 mg/kg bb
mg/kg bb dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 250
mg/kg bb
kontrol
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
mg/kg bb
berat badan
minggu II
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 500
mg/kg bb
dosis 250
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
-.56000
2.56501
.999
-.86000
2.56501
.997
-.12000
2.56501
1.000
1.70000
2.56501
.962
.56000
2.56501
.999
-.30000
2.56501
1.000
.44000
2.56501
1.000
2.00000
2.56501
.934
.86000
2.56501
.997
.30000
2.56501
1.000
.74000
2.56501
.998
1.26000
2.56501
.987
.12000
2.56501
1.000
-.44000
2.56501
1.000
-.74000
2.56501
.998
-.78000
3.12590
.999
-.38000
3.12590
1.000
3.00000
3.12590
.870
.72000
3.12590
.999
.78000
3.12590
.999
.40000
3.12590
1.000
3.78000
3.12590
.746
1.50000
3.12590
.988
.38000
3.12590
1.000
-.40000
3.12590
1.000
3.38000
3.12590
.814
1.10000
3.12590
.996
-3.00000
3.12590
.870
-3.78000
3.12590
.746
-3.38000
3.12590
.814
kontrol
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
8.2355
8.5355
7.7955
5.9755
7.1155
7.9755
7.2355
5.6755
6.8155
7.3755
6.9355
6.4155
7.5555
8.1155
8.4155
10.133
9
9.7339
6.3539
8.6339
8.5739
8.9539
5.5739
7.8539
8.9739
9.7539
5.9739
8.2539
12.353
9
13.133
9
12.733
9
7.1155
6.8155
7.5555
9.3755
8.2355
7.3755
8.1155
9.6755
8.5355
7.9755
8.4155
8.9355
7.7955
7.2355
6.9355
8.5739
8.9739
12.3539
10.0739
10.1339
9.7539
13.1339
10.8539
9.7339
8.9539
12.7339
10.4539
6.3539
5.5739
5.9739
94
Universitas Sumatera Utara
dosis 1000
mg/kg bb
-2.28000
3.12590
.947
-.72000
3.12590
.999
-1.50000
3.12590
.988
-1.10000
3.12590
.996
2.28000
3.12590
.947
-.58000
2.93275
1.000
-.24000
2.93275
1.000
2.58000
2.93275
.901
2.48000
2.93275
.913
.58000
2.93275
1.000
.34000
2.93275
1.000
3.16000
2.93275
.816
3.06000
2.93275
.832
.24000
2.93275
1.000
-.34000
2.93275
1.000
2.82000
2.93275
.869
2.72000
2.93275
.883
-2.58000
2.93275
.901
-3.16000
2.93275
.816
-2.82000
2.93275
.869
-.10000
2.93275
1.000
-2.48000
2.93275
.913
-3.06000
2.93275
.832
-2.72000
2.93275
.883
.10000
2.93275
1.000
.28000
3.14793
1.000
.70000
3.14793
.999
kontrol
dosis 250
dosis 1000 mg/kg bb
mg/kg bb
dosis 500
mg/kg bb
kontrol
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 500
mg/kg bb
berat badan
minggu III
dosis 250
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
dosis 1000 mg/kg bb
mg/kg bb
dosis 500
mg/kg bb
berat badan
kontrol
minggu Iv
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
11.633
9
10.073
9
10.853
9
10.453
9
7.0739
9.3559
9.0159
6.1959
6.2959
8.1959
8.4359
5.6159
5.7159
8.5359
9.1159
5.9559
6.0559
11.355
9
11.935
9
11.595
9
8.8759
11.255
9
11.835
9
11.495
9
8.6759
9.1398
8.7198
7.0739
8.6339
7.8539
8.2539
11.6339
8.1959
8.5359
11.3559
11.2559
9.3559
9.1159
11.9359
11.8359
9.0159
8.4359
11.5959
11.4959
6.1959
5.6159
5.9559
8.6759
6.2959
5.7159
6.0559
8.8759
9.6998
10.1198
95
Universitas Sumatera Utara
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
3.26000
3.14793
.836
3.36000
3.14793
.821
-.28000
3.14793
1.000
.42000
3.14793
1.000
2.98000
3.14793
.875
3.08000
3.14793
.862
-.70000
3.14793
.999
-.42000
3.14793
1.000
2.56000
3.14793
.923
2.66000
3.14793
.913
-3.26000
3.14793
.836
-2.98000
3.14793
.875
-2.56000
3.14793
.923
.10000
3.14793
1.000
-3.36000
3.14793
.821
-3.08000
3.14793
.862
-2.66000
3.14793
.913
-.10000
3.14793
1.000
kontrol
dosis 500
mg/kg bb
dosis 250
mg/kg bb
dosis 750
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 750
mg/kg bb
dosis 250
mg/kg bb
dosis 500
mg/kg bb
dosis 1000
mg/kg bb
kontrol
dosis 250
dosis 1000 mg/kg bb
mg/kg bb
dosis 500
mg/kg bb
dosis 750
mg/kg bb
6.1598
6.0598
9.6998
8.9998
6.4398
6.3398
10.119
8
9.8398
6.8598
6.7598
12.679
8
12.399
8
11.979
8
9.3198
12.779
8
12.499
8
12.079
8
9.5198
12.6798
12.7798
9.1398
9.8398
12.3998
12.4998
8.7198
8.9998
11.9798
12.0798
6.1598
6.4398
6.8598
9.5198
6.0598
6.3398
6.7598
9.3198
96
Universitas Sumatera Utara
Lampiran 15. Hasil analisis SPSS pakan mencit
Oneway
Descriptives
Jumlah pakan(Gram)
95% Confidence
Interval for Mean
Std.
N
Mean
Deviation
Std. Error
Lower
Upper
Minim
Maximu
Bound
Bound
um
m
kontrol
14
38,7929
2,33517
,62410
37,4446
40,1411
35,60
43,20
250 mg
14
39,9857
2,50564
,66966
38,5390
41,4324
35,40
43,20
500 mg
14
36,9571
3,66054
,97832
34,8436
39,0707
31,00
P42,00
750 mg
14
28,9714
7,88059
2,10618
24,4213
33,5215
20,50
46,10
1000 mg
14
24,1286
9,61733
2,57034
18,5757
29,6815
14,80
44,80
Total
70
33,7671
8,50961
1,01709
31,7381
35,7962
14,80
46,10
ANOVA
Jumlah pakan(Gram)
Sum of Squares
df
Mean Square
F
Between Groups
2660,077
4
665,019
Within Groups
2336,458
65
35,946
Total
4996,534
69
Sig.
18,501
,000
Post Hoc Tests
Multiple Comparisons
Dependent Variable: Jumlah pakan(Gram)
(I)
(J)
Perlakua Perlaku
Tukey HSD
95% Confidence Interval
Mean
Difference
Std.
(I-J)
Error
Lower
n
an
kontrol
250 mg
-1,19286
2,26607
,984
-7,5511
5,1653
500 mg
1,83571
2,26607
,927
-4,5225
8,1939
750 mg
9,82143
*
2,26607
,000
3,4632
16,1796
*
2,26607
,000
8,3061
21,0225
kontrol
1,19286
2,26607
,984
-5,1653
7,5511
500 mg
3,02857
2,26607
,670
-3,3296
9,3868
750 mg
11,01429
*
2,26607
,000
4,6561
17,3725
15,85714
*
2,26607
,000
9,4989
22,2153
kontrol
-1,83571
2,26607
,927
-8,1939
4,5225
250 mg
-3,02857
2,26607
,670
-9,3868
3,3296
1000 mg
250 mg
1000 mg
500 mg
14,66429
Sig.
Bound
Upper Bound
97
Universitas Sumatera Utara
*
2,26607
,007
1,6275
14,3439
*
2,26607
,000
6,4704
19,1868
*
2,26607
,000
-16,1796
-3,4632
*
2,26607
,000
-17,3725
-4,6561
*
2,26607
,007
-14,3439
-1,6275
4,84286
2,26607
,217
-1,5153
11,2011
-14,66429
*
2,26607
,000
-21,0225
-8,3061
250 mg
-15,85714
*
2,26607
,000
-22,2153
-9,4989
500 mg
-12,82857
*
2,26607
,000
-19,1868
-6,4704
750 mg
-4,84286
2,26607
,217
-11,2011
1,5153
250 mg
-1,19286
2,26607
,600
-5,7185
3,3328
500 mg
1,83571
2,26607
,421
-2,6899
6,3614
750 mg
*
2,26607
,000
5,2958
14,3471
*
2,26607
,000
10,1386
19,1899
kontrol
1,19286
2,26607
,600
-3,3328
5,7185
500 mg
3,02857
2,26607
,186
-1,4971
7,5542
750 mg
11,01429
*
2,26607
,000
6,4886
15,5399
15,85714
*
2,26607
,000
11,3315
20,3828
kontrol
-1,83571
2,26607
,421
-6,3614
2,6899
250 mg
-3,02857
2,26607
,186
-7,5542
1,4971
750 mg
*
2,26607
,001
3,4601
12,5114
*
2,26607
,000
8,3029
17,3542
*
2,26607
,000
-14,3471
-5,2958
*
2,26607
,000
-15,5399
-6,4886
-7,98571
*
2,26607
,001
-12,5114
-3,4601
4,84286
*
2,26607
,036
,3172
9,3685
-14,66429
*
2,26607
,000
-19,1899
-10,1386
-15,85714
*
2,26607
,000
-20,3828
-11,3315
-12,82857
*
2,26607
,000
-17,3542
-8,3029
*
2,26607
,036
-9,3685
-,3172
750 mg
750 mg
1000 mg
12,82857
kontrol
-9,82143
250 mg
-11,01429
500 mg
-7,98571
1000 mg
1000 mg kontrol
LSD
kontrol
1000 mg
250 mg
1000 mg
500 mg
1000 mg
750 mg
7,98571
kontrol
250 mg
500 mg
1000 mg
1000 mg kontrol
250 mg
500 mg
750 mg
9,82143
14,66429
7,98571
12,82857
-9,82143
-11,01429
-4,84286
*. The mean difference is significant at the 0.05 level.
98
Universitas Sumatera Utara
Lampiran 16. Hasil analisis SPSS kadar ALT dan kreatinin
Descriptives
95% Confidence Interval for
N
Mean
Std.
Std.
Deviation
Error
Mean
Lower Bound
Upper Bound
Min
Max
kadar
kontrol
5
24,2600
7,67222 3,43112
14,7337
33,7863
11,90
31,60
ALT
250 mg
5
48,5000
7,00321 3,13193
39,8044
57,1956
36,60
55,10
500 mg
4
73,5000
19,46244 9,73122
42,5309
104,4691
55,80
92,00
750 mg
3
99,5000
12,99038 7,50000
67,2301
131,7699
92,00
114,50
2
151,1000
153,6412 150,90
151,30
19
66,2368
1000
,28284
,20000
148,5588
40,97605 9,40055
46,4870
85,9867
11,90
151,30
mg
Total
kadar
kontrol
5
,1200
,04637
,02074
,0624
,1776
,05
,17
kreati
250 mg
5
,1840
,04450
,01990
,1287
,2393
,13
,23
500 mg
4
,3325
,05252
,02626
,2489
,4161
,30
,41
750 mg
3
,4667
,04933
,02848
,3441
,5892
,41
,50
2
,7100
,08485
,06000
-,0524
1,4724
,65
,77
19
,2984
,19577
,04491
,2041
,3928
,05
,77
nin
1000
mg
Total
Tests of Normality
a
Kolmogorov-Smirnov
Perlakuan
kadar
kreatinin
kadar ALT
Statistic
kontrol
df
,267
Shapiro-Wilk
Sig.
5
Statistic
df
Sig.
,200
*
,930
5
,593
*
,877
5
,298
250 mg
,249
5
,200
500 mg
,344
4
.
,753
4
,041
750 mg
,349
3
.
,832
3
,194
1000 mg
,260
2
.
kontrol
,249
5
,200
*
,898
5
,399
250 mg
,362
5
,031
,823
5
,123
500 mg
,293
4
.
,794
4
,092
750 mg
,385
3
.
,750
3
,000
1000 mg
,260
2
.
*. This is a lower bound of the true significance.
a. Lilliefors Significance Correction
99
Universitas Sumatera Utara
Lampiran 16.(lanjutan)
NPAR TESTS
/K-W=kadar kdr BY perlakuan(1 5)
/MISSING ANALYSIS.
NPar Tests
Kruskal-Wallis Test
Ranks
Perlakuan
kadar ALT
N
Kontrol
5
3,00
250 mg
5
8,00
500 mg
4
12,75
750 mg
3
15,67
1000 mg
2
18,50
Total
kadar kreatinin
Mean Rank
19
Kontrol
5
3,90
250 mg
5
7,10
500 mg
4
12,63
750 mg
3
15,83
1000 mg
2
18,50
Total
19
a,b
Test Statistics
kadar ALT
Chi-Square
16,989
15,930
4
4
,002
,003
Df
Asymp. Sig.
kadar kreatinin
a. Kruskal Wallis Test
b. Grouping Variable: Perlakuan
100
Universitas Sumatera Utara
NPAR TESTS
/M-W= kadar kdr BY perlakuan(1 2)
/MISSING ANALYSIS.
NPar Tests
Mann-Whitney Test
Ranks
Perlakuan
kadar ALT
N
Mean Rank
kontrol
5
3,00
15,00
250 mg
5
8,00
40,00
Total
kadar kreatinin
Sum of Ranks
10
kontrol
5
3,90
19,50
250 mg
5
7,10
35,50
Total
10
a
Test Statistics
kadar ALT
Mann-Whitney U
kadar kreatinin
,000
4,500
Wilcoxon W
15,000
19,500
Z
-2,611
-1,687
,009
,092
Asymp. Sig. (2-tailed)
Exact Sig. [2*(1-tailed Sig.)]
,008
b
,095
b
a. Grouping Variable: Perlakuan
b. Not corrected for ties.
NPAR TESTS
/M-W= kadar kdr BY perlakuan(1 3)
/MISSING ANALYSIS.
NPar Tests
Mann-Whitney Test
Ranks
Perlakuan
kadar ALT
kadar kreatinin
N
Mean Rank
Sum of Ranks
kontrol
5
3,00
15,00
500 mg
4
7,50
30,00
Total
9
kontrol
5
3,00
15,00
500 mg
4
7,50
30,00
Total
9
101
Universitas Sumatera Utara
a
Test Statistics
kadar ALT
Mann-Whitney U
kadar kreatinin
,000
,000
Wilcoxon W
15,000
15,000
Z
-2,449
-2,470
,014
,014
Asymp. Sig. (2-tailed)
Exact Sig. [2*(1-tailed Sig.)]
,016
b
,016
b
a. Grouping Variable: Perlakuan
b. Not corrected for ties.
NPAR TESTS
/K-W=kadar kdr BY perlakuan(1 4)
/STATISTICS DESCRIPTIVES
/MISSING ANALYSIS.
NPar Tests
Kruskal-Wallis Test
Ranks
Perlakuan
kadar ALT
N
Kontrol
5
3,00
250 mg
5
8,00
500 mg
4
12,75
750 mg
3
15,67
Total
kadar kreatinin
Mean Rank
17
Kontrol
5
3,90
250 mg
5
7,10
500 mg
4
12,63
750 mg
3
15,83
Total
17
a,b
Test Statistics
kadar ALT
Chi-Square
14,762
13,445
3
3
,002
,004
Df
Asymp. Sig.
kadar kreatinin
a. Kruskal Wallis Test
b. Grouping Variable: Perlakuan
102
Universitas Sumatera Utara
NPAR TESTS
/M-W= kadar kdr BY perlakuan(1 5)
/MISSING ANALYSIS.
NPar Tests
Mann-Whitney Test
Ranks
Perlakuan
kadar ALT
kadar kreatinin
N
Mean Rank
Sum of Ranks
Kontrol
5
3,00
15,00
1000 mg
3
7,00
21,00
Total
8
Kontrol
5
3,00
15,00
1000 mg
3
7,00
21,00
Total
8
a
Test Statistics
kadar ALT
Mann-Whitney U
kadar kreatinin
,000
,000
Wilcoxon W
15,000
15,000
Z
-2,249
-2,263
,024
,024
Asymp. Sig. (2-tailed)
Exact Sig. [2*(1-tailed Sig.)]
,036
b
,036
b
a. Grouping Variable: Perlakuan
b. Not corrected for ties.
103
Universitas Sumatera Utara