Formulasi dan Eveluasi Gel Ekstrak Benalu Kopi (Scurrula ferruginea (Jack) Danser) Sebagai Anti-Aging
44
Universitas Sumatera Utara
Lampiran 2. Gambar tumbuhan segar benalu kopi dan simplisia benalu kopi
Benalu kopi segar
Simplisia Benalu kopi
45
Universitas Sumatera Utara
Lampiran 3.Serbuk simplisia benalu kopi dan hasil ekstrak
Serbuk simplisia benalu kopi
ekstrak etanol benalu kopi
46
Universitas Sumatera Utara
Lampiran 4. Gambar alat yang digunakan
pH meter (Exact Instrument)
Moisturizer checker(Aramo)
viscometer Brookfield
Skin analyzer(Aramo)
47
Universitas Sumatera Utara
Lampiran 5. Gambar sediaan gel
Gel setelah dimasukkan ke dalam wadah
Gel setelah 90 hari
48
Universitas Sumatera Utara
Lampiran 6. Gambar hasil pengamatan homogenitas
49
Universitas Sumatera Utara
Lampiran 7. Bagan kerja pembuatan simplisia benalu kopi
Benalu kopi
-
Dibersihkan dari kotoran menggunakan
air mengalir hingga bersih
-
Ditiriskan
-
Dikeringkan
Simplisia benalu kopi
-
Diserbukkan
blender
dengan
menggunakan
Serbuk simplisia Benalu kopi
50
Universitas Sumatera Utara
Lampiran 8. Bagan pembuatan ekstrak benalu kopi
Serbuk simplisia benalu kopi 900 gram
- dimasukkan ke dalam bejana
- dimaserasi dengan 75 bagian etanol 96%
- ditutup wadah dan dibiarkan selama 5 hari
sambil sering di aduk
- dipisahkan maserat dan ampas
- dimaserasi kembali ampas dengan pelarut
etanol 96% sebanyak 25 bagian dan
didiamkan selama 2 hari
- disaring
- digabung semua hasil maserat
Maserat
- diuapkan dengan rotary evaporator pada
suhu 40-50°C
- dipekatkan di atas penangas air
Ekstrak kental 60 g
51
Universitas Sumatera Utara
Lampiran 9. Bagan pembuatan basis gel
Air suling
Ditimbang sebanyak 20 kali berat
Carbomer
Didihkan
Dimasukkan ke dalam lumpang
Ditaburkancarbomersecara
diatasnya
merata
Ditambahkanmetilparaben dan propil
paraben yang telah dilarutkanterlebih
dahulu dengan propilen glikol gerus
homogen
Ditambahkan
sisa
yangdibutuhkan
air
suling
Basis gel
52
Universitas Sumatera Utara
Lampiran 10. Bagan kerja pembuatan, evaluasi penentuan mutu fisik sediaan gel
ekstrak benalu kopi
Ekstrak benalu kopi
Basis gel
Ditimbang masing-masing
Ditimbang
tiapkonsentrasi
Dimasukkan ke dalam lumpang
Bagian I
Bagian II
Dicampur dan digerus homogen
Campuran bagian I dan II
Dimasukkan ke dalam wadah
Sediaan gel
Penentuan mutu fisik:
a. Stabilitas
b. Homogenitas
c. Ph
d. Viskositas
e. Uji iritasi
f. Uji efek anti-aging (Skin
analyzer)
53
Universitas Sumatera Utara
Lampiran 11.
Contoh surat pernyataan sukarelawan yang ikut serta dalam
penelitian
SURAT PERNYATAAN PERSETUJUAN IKUT SERTA DALAM
PENELITIAN
Saya yang bertandatangan dibawah ini:
Nama
:
Umur
:
Alamat
:
Telah mendapat penjelasan secukupnya bahwa saya akan melakukan uji
gel ekstrak etanol benalu kopi terhadap kulit sebagai sediaan gel anti-aging.
Setelah mendapatkan penjelasan secukupnya tentang manfaat penelitian ini dan
efek sampingnya, maka saya menyatakan SETUJU
untuk ikut serta dalam
penelitian dari Ester Pasaribu dengan judul “FORMULASI DAN EVALUASI
GEL EKSTRAK BENALU KOPI (Scurrula ferruginea (Jack) Danser) SEBAGAI
ANTI-AGING”, sebagai usaha untuk mengetahui apakah sediaan gel dari ekstrak
benalu kopi yang dihasilkan mampu memberikan efek anti penuaan dini. Saya
menyatakan sukarela dan bersedia untuk mengikuti prosedur penelitian yang telah
ditetapkan.
Persetujuan ini saya buat dengan penuh kesadaran dan tanpa paksaan dari
pihak manapun. Demikian surat pernyataan ini dibuat untuk dapat dipergunakan
sebagaimana mestinya.
Medan,
Mei 2016
Peneliti,
Sukarelawan,
( Ester Pasaribu)
(Ira Maya Sinaga)
54
Universitas Sumatera Utara
Lampiran 12. Contoh hasil pengukuran menggunakan alat Skin analyzerpada
Kulit punggung sukarelawan
Kadar air
Evennes/pori
55
Universitas Sumatera Utara
Lampira 12 (Lanjutan)
Spot (Noda)
Keriput
56
Universitas Sumatera Utara
Lampiran 13.
Data hasil uji statistik kadar air, evennes, pori, spot, dan
keriputgel ekstrak benalu kopi
Kadar air
One-Sample Kolmogorov-Smirnov Test
Minggu_
Minggu_
Minggu_
Minggu_
Minggu_
awal
I
II
III
IV
N
15
15
15
15
15
30,8667
31,9333
32,7333
33,3333
34,1333
,91548
1,33452
1,66762
1,95180
2,32584
Absolute
,242
,158
,137
,166
,210
Positive
,242
,158
,137
,101
,111
Negative
-,172
-,121
-,130
-,166
-,210
Kolmogorov-Smirnov Z
,938
,611
,529
,641
,815
Asymp. Sig. (2-tailed)
,343
,849
,942
,806
,520
a,b
Normal Parameters
Mean
Std. Deviation
Most Extreme Differences
a. Test distribution is Normal.
ANOVA
Sum of Squares
Minggu_awal
Minggu_I
Between Groups
Minggu_III
4
,433
Within Groups
10,000
10
1,000
Total
11,733
14
Between Groups
16,267
4
4,067
8,667
10
,867
Total
24,933
14
Between Groups
26,267
4
6,567
Within Groups
12,667
10
1,267
Total
38,933
14
Between Groups
44,000
4
11,000
9,333
10
,933
Total
53,333
14
Between Groups
70,400
4
17,600
5,333
10
,533
75,733
14
Within Groups
Minggu_IV
Mean Square
1,733
Within Groups
Minggu_II
df
Within Groups
Total
F
Sig.
,433
,782
4,692
,022
5,184
,016
11,786
,001
33,000
,000
57
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Minggu_awal
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Formula 0
3
30,3333
Formula 1
3
30,6667
Formula 2
3
31,0000
Formula 3
3
31,0000
Formula 4
3
31,3333
Sig.
,738
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_I
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 0
3
30,3333
Formula 1
3
31,3333
31,3333
Formula 2
3
32,0000
32,0000
Formula 3
3
32,6667
32,6667
Formula 4
3
33,3333
Sig.
,070
,137
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_II
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 0
3
30,3333
Formula 1
3
32,3333
32,3333
Formula 2
3
33,3333
33,3333
Formula 3
3
33,6667
Formula 4
3
34,0000
Sig.
,052
,417
Means for groups in homogeneous subsets are displayed.
58
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Minggu_III
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 0
3
30,3333
Formula 1
3
33,0000
Formula 2
3
33,3333
Formula 3
3
35,0000
Formula 4
3
35,0000
Sig.
1,000
,158
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_IV
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 0
3
Formula 1
3
33,6667
Formula 2
3
34,3333
Formula 3
3
Formula 4
3
Sig.
4
30,3333
34,3333
35,6667
35,6667
36,6667
1,000
,794
,242
,487
Means for groups in homogeneous subsets are displayed.
a.
Uses Harmonic Mean Sample Size = 3,000.
59
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Evennes
One-Sample Kolmogorov-Smirnov Test
Mingguaw
al
N
a,b
Normal Parameters
Minggu I
Minggu
Minggu
Minggu
II
III
IV
15
15
15
15
15
Mean
39,3333
37,3333
35,9333
34,1333
32,5333
Std. Deviation
1,04654
2,05866
2,65832
3,41983
4,29063
Most Extreme
Absolute
,225
,173
,237
,230
,190
Differences
Positive
,225
,173
,237
,230
,190
Negative
-,175
-,102
-,135
-,123
-,145
Kolmogorov-Smirnov Z
,871
,670
,919
,890
,736
Asymp. Sig. (2-tailed)
,434
,760
,367
,407
,651
a. Test distribution is Normal.
ANOVA
Sum of Squares
Mingguawal
Minggupertama
6,667
4
1,667
Within Groups
8,667
10
,867
Total
15,333
14
Between Groups
52,667
4
13,167
6,667
10
,667
Total
59,333
14
Between Groups
90,267
4
22,567
8,667
10
,867
98,933
14
151,067
4
37,767
12,667
10
1,267
Total
163,733
14
Between Groups
245,733
4
61,433
12,000
10
1,200
257,733
14
Within Groups
Total
Mingguketiga
Between Groups
Within Groups
Minggukeempat
Mean Square
Between Groups
Within Groups
Minggukedua
df
Within Groups
Total
F
Sig.
1,923
,183
19,750
,000
26,038
,000
29,816
,000
51,194
,000
60
Universitas Sumatera Utara
Lampiran 13 (Lanjutan)
Minggua_awal
a
Tukey HSD
kelompok
Subset for alpha = 0.05
N
1
Formula 1
3
38,3333
Formula 3
3
39,0000
Formula 2
3
39,3333
Formula 4
3
39,6667
Formula 0
3
40,3333
Sig.
,137
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_I
Tukey HSDa
kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
34,6667
Formula 3
3
36,3333
Formula 2
3
37,3333
Formula 1
3
38,0000
Formula 0
3
36,3333
40,3333
Sig.
,166
,166
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_II
a
Tukey HSD
kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
33,3333
Formula 3
3
34,3333
34,3333
Formula 2
3
35,0000
35,0000
Formula 1
3
Formula 0
3
Sig.
36,6667
40,3333
,257
,070
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
61
Universitas Sumatera Utara
Lampiran 13 (Lanjutan)
Minggu_III
a
Tukey HSD
kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
30,6667
Formula 3
3
32,3333
32,3333
Formula 2
3
33,3333
33,3333
Formula 1
3
Formula 0
3
34,3333
40,0000
Sig.
,091
,263
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_IV
a
Tukey HSD
kelompok
Subset for alpha = 0.05
N
1
2
Formula 4
3
28,6667
Formula 3
3
29,6667
Formula 2
3
31,0000
Formula 1
3
Formula 0
3
Sig.
3
31,0000
33,3333
40,0000
,142
,142
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
62
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Pori
One-Sample Kolmogorov-Smirnov Test
N
Minggu_
Minggu_
Minggu_
Minggu
Minggu_
awal
I
II
III
IV
15
15
15
15
15
Mean
25,8000
23,9333
22,6667
21,0000
19,6000
Std.
1,14642
2,12020
2,82000
3,60555
3,81351
Absolute
,236
,197
,215
,167
,142
Positive
,148
,154
,118
,124
,125
Negative
-,236
-,197
-,215
-,167
-,142
Kolmogorov-Smirnov Z
,914
,761
,833
,645
,549
Asymp. Sig. (2-tailed)
,374
,608
,491
,799
,924
a,b
Normal Parameters
Deviation
Most Extreme Differences
a. Test distribution is Normal.
ANOVA
Sum of Squares
Minggu_awal
Minggu_I
Minggu_II
Minggu_III
Between Groups
Mean Square
,400
4
,100
Within Groups
18,000
10
1,800
Total
18,400
14
Between Groups
44,933
4
11,233
Within Groups
18,000
10
1,800
Total
62,933
14
Between Groups
92,000
4
23,000
Within Groups
19,333
10
1,933
Total
111,333
14
Between Groups
154,000
4
38,500
28,000
10
2,800
Total
182,000
14
Between Groups
184,267
4
46,067
19,333
10
1,933
203,600
14
Within Groups
Minggu_IV
df
Within Groups
Total
F
Sig.
,056
,993
6,241
,009
11,897
,001
13,750
,000
23,828
,000
63
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Minggu_awal
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Formula 0
3
25,6667
Formula 3
3
25,6667
Formula 4
3
25,6667
Formula 1
3
26,0000
Formula 2
3
26,0000
Sig.
,998
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_I
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 4
3
21,0000
Formula 3
3
23,3333
23,3333
Formula 2
3
24,0000
24,0000
Formula 0
3
25,6667
Formula 1
3
25,6667
Sig.
,117
,280
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_II
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 4
3
Formula 3
3
22,3333
Formula 2
3
22,6667
Formula 1
3
24,3333
Formula 0
3
25,6667
Sig.
18,3333
1,000
,087
Means for groups in homogeneous subsets are displayed.
a.
Uses Harmonic Mean Sample
64
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Minggu_III
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
15,6667
Formula 3
3
20,6667
Formula 2
3
21,0000
Formula 1
3
22,0000
Formula 0
3
22,0000
25,6667
Sig.
1,000
,860
,127
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_IV
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
14,3333
Formula 3
3
18,0000
Formula 2
3
19,6667
Formula 1
3
21,0000
Formula 0
3
Sig.
18,0000
25,0000
,055
,135
1,000
Means for groups in homogeneous subsets are displayed.
65
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Spot
One-Sample Kolmogorov-Smirnov Test
N
Normal Parametersa,b
Most Extreme Differences
Mean
Std.
Deviation
Absolute
Positive
Negative
Mingguaw
al
Minggu I
15
15
42,8000 40,1333
1,32017 2,55976
,173
,173
-,160
,671
,760
Kolmogorov-Smirnov Z
Asymp. Sig. (2-tailed)
,187
,187
-,136
,726
,668
Minggu
II
15
36,1333
5,13902
Minggu
III
15
32,4000
7,89032
Minggu
IV
15
29,7333
9,27721
,158
,158
-,156
,613
,847
,120
,120
-,096
,466
,982
,132
,132
-,107
,509
,958
a. Test distribution is Normal.
ANOVA
Sum of Squares
Mingguawal
Minggu I
Minggu II
Between Groups
4
1,767
Within Groups
17,333
10
1,733
Total
24,400
14
Between Groups
71,067
4
17,767
Within Groups
20,667
10
2,067
Total
91,733
14
343,733
4
85,933
26,000
10
2,600
Total
369,733
14
Between Groups
842,933
4
210,733
28,667
10
2,867
871,600
14
1178,933
4
Between Groups
Within Groups
Total
Minggu IV
Mean Square
7,067
Within Groups
Minggu III
df
Between Groups
294,733
F
Sig.
1,019
,443
8,597
,003
33,051
,000
73,512
,000
113,35
,000
9
Within Groups
Total
26,000
10
1204,933
14
2,600
66
Universitas Sumatera Utara
Lampira 13(Lanjutan)
Minggu_awal
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Formula 2
3
42,0000
Formula 4
3
42,3333
Formula 3
3
42,6667
Formula 1
3
43,0000
Formula 0
3
44,0000
Sig.
,394
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_I
Tukey HSDa
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 4
3
37,3333
Formula 2
3
39,3333
Formula 3
3
39,6667
Formula 1
3
40,3333
Formula 0
3
40,3333
44,0000
Sig.
,153
,065
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_II
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
Formula 3
3
34,0000
Formula 2
3
36,3333
Formula 1
3
37,6667
Formula 0
3
Sig.
29,0000
43,6667
1,000
,109
1,000
Means for groups in homogeneous subsets are displayed.
67
Universitas Sumatera Utara
Minggu_II
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
29,0000
Formula 3
3
34,0000
Formula 2
3
36,3333
Formula 1
3
37,6667
Formula 0
3
43,6667
Sig.
1,000
,109
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_III
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
Formula 3
3
29,6667
Formula 2
3
31,3333
Formula 1
3
Formula 0
3
4
21,0000
36,3333
43,6667
Sig.
1,000
,749
1,000
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_IV
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
Formula 3
3
24,3333
Formula 2
3
27,6667
Formula 1
3
Formula 0
3
Sig.
4
18,0000
35,0000
43,6667
1,000
,159
1,000
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
68
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Keriput
One-Sample Kolmogorov-Smirnov Test
Minggua
wal
N
MingguI
MingguI
Minggu
I
II
IV
Minggu I
15
15
15
15
15
24,2667
22,6667
21,7333
21,2667
20,5333
,88372
1,91485
2,65832
2,98727
3,44065
Absolute
,219
,118
,142
,131
,114
Positive
,219
,118
,142
,109
,114
Negative
-,197
-,102
-,136
-,131
-,110
Kolmogorov-Smirnov Z
,847
,458
,550
,508
,443
Asymp. Sig. (2-tailed)
,471
,985
,923
,959
,989
a,b
Normal Parameters
Mean
Std. Deviation
Most Extreme Differences
a. Test distribution is Normal.
ANOVA
Sum of Squares
Minggu awal
Minggu I
Minggu II
Minggu III
Mean Square
F
Sig.
,654
,637
10,333 10,333
,001
Between Groups
2,267
4
,567
Within Groups
8,667
10
,867
Total
10,933
14
Between Groups
41,333
4
Within Groups
10,000
10
Total
51,333
14
Between Groups
80,933
4
Within Groups
18,000
10
Total
98,933
14
108,933
4
16,000
10
Total
124,933
14
Between Groups
149,733
4
16,000
10
165,733
14
Between Groups
Within Groups
Minggu IV
df
Within Groups
Total
1,000
20,233 11,241
,001
1,800
27,233 17,021
,000
1,600
37,433 23,396
,000
1,600
69
Universitas Sumatera Utara
Lampiran 13 (Lanjutan)
Minggu_awal
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Formula 1
3
24,0000
Formula 3
3
24,0000
Formula 4
3
24,0000
Formula 2
3
24,3333
Formula 0
3
25,0000
Sig.
,689
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_I
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
20,3333
Formula 3
3
21,3333
21,3333
Formula 2
3
23,0000
23,0000
23,0000
Formula 1
3
23,6667
23,6667
Formula 0
3
25,0000
Sig.
,052
,098
,179
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_II
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 4
3
18,6667
Formula 3
3
19,6667
Formula 2
3
22,0000
Formula 1
3
23,3333
Formula 0
3
25,0000
Sig.
,073
22,0000
,117
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
70
Universitas Sumatera Utara
Lampiran 13 (Lanjutan)
Minggu_III
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
17,3333
Formula 3
3
19,3333
Formula 2
3
Formula 1
3
23,0000
Formula 0
3
25,0000
19,3333
21,6667
Sig.
,359
21,6667
,235
,055
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_IV
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 4
3
16,0000
Formula 3
3
18,3333
Formula 2
3
Formula 1
3
Formula 0
3
Sig.
3
4
18,3333
20,6667
20,6667
22,6667
22,6667
25,0000
,235
,235
,359
,235
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
71
Universitas Sumatera Utara
Universitas Sumatera Utara
Lampiran 2. Gambar tumbuhan segar benalu kopi dan simplisia benalu kopi
Benalu kopi segar
Simplisia Benalu kopi
45
Universitas Sumatera Utara
Lampiran 3.Serbuk simplisia benalu kopi dan hasil ekstrak
Serbuk simplisia benalu kopi
ekstrak etanol benalu kopi
46
Universitas Sumatera Utara
Lampiran 4. Gambar alat yang digunakan
pH meter (Exact Instrument)
Moisturizer checker(Aramo)
viscometer Brookfield
Skin analyzer(Aramo)
47
Universitas Sumatera Utara
Lampiran 5. Gambar sediaan gel
Gel setelah dimasukkan ke dalam wadah
Gel setelah 90 hari
48
Universitas Sumatera Utara
Lampiran 6. Gambar hasil pengamatan homogenitas
49
Universitas Sumatera Utara
Lampiran 7. Bagan kerja pembuatan simplisia benalu kopi
Benalu kopi
-
Dibersihkan dari kotoran menggunakan
air mengalir hingga bersih
-
Ditiriskan
-
Dikeringkan
Simplisia benalu kopi
-
Diserbukkan
blender
dengan
menggunakan
Serbuk simplisia Benalu kopi
50
Universitas Sumatera Utara
Lampiran 8. Bagan pembuatan ekstrak benalu kopi
Serbuk simplisia benalu kopi 900 gram
- dimasukkan ke dalam bejana
- dimaserasi dengan 75 bagian etanol 96%
- ditutup wadah dan dibiarkan selama 5 hari
sambil sering di aduk
- dipisahkan maserat dan ampas
- dimaserasi kembali ampas dengan pelarut
etanol 96% sebanyak 25 bagian dan
didiamkan selama 2 hari
- disaring
- digabung semua hasil maserat
Maserat
- diuapkan dengan rotary evaporator pada
suhu 40-50°C
- dipekatkan di atas penangas air
Ekstrak kental 60 g
51
Universitas Sumatera Utara
Lampiran 9. Bagan pembuatan basis gel
Air suling
Ditimbang sebanyak 20 kali berat
Carbomer
Didihkan
Dimasukkan ke dalam lumpang
Ditaburkancarbomersecara
diatasnya
merata
Ditambahkanmetilparaben dan propil
paraben yang telah dilarutkanterlebih
dahulu dengan propilen glikol gerus
homogen
Ditambahkan
sisa
yangdibutuhkan
air
suling
Basis gel
52
Universitas Sumatera Utara
Lampiran 10. Bagan kerja pembuatan, evaluasi penentuan mutu fisik sediaan gel
ekstrak benalu kopi
Ekstrak benalu kopi
Basis gel
Ditimbang masing-masing
Ditimbang
tiapkonsentrasi
Dimasukkan ke dalam lumpang
Bagian I
Bagian II
Dicampur dan digerus homogen
Campuran bagian I dan II
Dimasukkan ke dalam wadah
Sediaan gel
Penentuan mutu fisik:
a. Stabilitas
b. Homogenitas
c. Ph
d. Viskositas
e. Uji iritasi
f. Uji efek anti-aging (Skin
analyzer)
53
Universitas Sumatera Utara
Lampiran 11.
Contoh surat pernyataan sukarelawan yang ikut serta dalam
penelitian
SURAT PERNYATAAN PERSETUJUAN IKUT SERTA DALAM
PENELITIAN
Saya yang bertandatangan dibawah ini:
Nama
:
Umur
:
Alamat
:
Telah mendapat penjelasan secukupnya bahwa saya akan melakukan uji
gel ekstrak etanol benalu kopi terhadap kulit sebagai sediaan gel anti-aging.
Setelah mendapatkan penjelasan secukupnya tentang manfaat penelitian ini dan
efek sampingnya, maka saya menyatakan SETUJU
untuk ikut serta dalam
penelitian dari Ester Pasaribu dengan judul “FORMULASI DAN EVALUASI
GEL EKSTRAK BENALU KOPI (Scurrula ferruginea (Jack) Danser) SEBAGAI
ANTI-AGING”, sebagai usaha untuk mengetahui apakah sediaan gel dari ekstrak
benalu kopi yang dihasilkan mampu memberikan efek anti penuaan dini. Saya
menyatakan sukarela dan bersedia untuk mengikuti prosedur penelitian yang telah
ditetapkan.
Persetujuan ini saya buat dengan penuh kesadaran dan tanpa paksaan dari
pihak manapun. Demikian surat pernyataan ini dibuat untuk dapat dipergunakan
sebagaimana mestinya.
Medan,
Mei 2016
Peneliti,
Sukarelawan,
( Ester Pasaribu)
(Ira Maya Sinaga)
54
Universitas Sumatera Utara
Lampiran 12. Contoh hasil pengukuran menggunakan alat Skin analyzerpada
Kulit punggung sukarelawan
Kadar air
Evennes/pori
55
Universitas Sumatera Utara
Lampira 12 (Lanjutan)
Spot (Noda)
Keriput
56
Universitas Sumatera Utara
Lampiran 13.
Data hasil uji statistik kadar air, evennes, pori, spot, dan
keriputgel ekstrak benalu kopi
Kadar air
One-Sample Kolmogorov-Smirnov Test
Minggu_
Minggu_
Minggu_
Minggu_
Minggu_
awal
I
II
III
IV
N
15
15
15
15
15
30,8667
31,9333
32,7333
33,3333
34,1333
,91548
1,33452
1,66762
1,95180
2,32584
Absolute
,242
,158
,137
,166
,210
Positive
,242
,158
,137
,101
,111
Negative
-,172
-,121
-,130
-,166
-,210
Kolmogorov-Smirnov Z
,938
,611
,529
,641
,815
Asymp. Sig. (2-tailed)
,343
,849
,942
,806
,520
a,b
Normal Parameters
Mean
Std. Deviation
Most Extreme Differences
a. Test distribution is Normal.
ANOVA
Sum of Squares
Minggu_awal
Minggu_I
Between Groups
Minggu_III
4
,433
Within Groups
10,000
10
1,000
Total
11,733
14
Between Groups
16,267
4
4,067
8,667
10
,867
Total
24,933
14
Between Groups
26,267
4
6,567
Within Groups
12,667
10
1,267
Total
38,933
14
Between Groups
44,000
4
11,000
9,333
10
,933
Total
53,333
14
Between Groups
70,400
4
17,600
5,333
10
,533
75,733
14
Within Groups
Minggu_IV
Mean Square
1,733
Within Groups
Minggu_II
df
Within Groups
Total
F
Sig.
,433
,782
4,692
,022
5,184
,016
11,786
,001
33,000
,000
57
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Minggu_awal
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Formula 0
3
30,3333
Formula 1
3
30,6667
Formula 2
3
31,0000
Formula 3
3
31,0000
Formula 4
3
31,3333
Sig.
,738
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_I
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 0
3
30,3333
Formula 1
3
31,3333
31,3333
Formula 2
3
32,0000
32,0000
Formula 3
3
32,6667
32,6667
Formula 4
3
33,3333
Sig.
,070
,137
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_II
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 0
3
30,3333
Formula 1
3
32,3333
32,3333
Formula 2
3
33,3333
33,3333
Formula 3
3
33,6667
Formula 4
3
34,0000
Sig.
,052
,417
Means for groups in homogeneous subsets are displayed.
58
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Minggu_III
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 0
3
30,3333
Formula 1
3
33,0000
Formula 2
3
33,3333
Formula 3
3
35,0000
Formula 4
3
35,0000
Sig.
1,000
,158
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_IV
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 0
3
Formula 1
3
33,6667
Formula 2
3
34,3333
Formula 3
3
Formula 4
3
Sig.
4
30,3333
34,3333
35,6667
35,6667
36,6667
1,000
,794
,242
,487
Means for groups in homogeneous subsets are displayed.
a.
Uses Harmonic Mean Sample Size = 3,000.
59
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Evennes
One-Sample Kolmogorov-Smirnov Test
Mingguaw
al
N
a,b
Normal Parameters
Minggu I
Minggu
Minggu
Minggu
II
III
IV
15
15
15
15
15
Mean
39,3333
37,3333
35,9333
34,1333
32,5333
Std. Deviation
1,04654
2,05866
2,65832
3,41983
4,29063
Most Extreme
Absolute
,225
,173
,237
,230
,190
Differences
Positive
,225
,173
,237
,230
,190
Negative
-,175
-,102
-,135
-,123
-,145
Kolmogorov-Smirnov Z
,871
,670
,919
,890
,736
Asymp. Sig. (2-tailed)
,434
,760
,367
,407
,651
a. Test distribution is Normal.
ANOVA
Sum of Squares
Mingguawal
Minggupertama
6,667
4
1,667
Within Groups
8,667
10
,867
Total
15,333
14
Between Groups
52,667
4
13,167
6,667
10
,667
Total
59,333
14
Between Groups
90,267
4
22,567
8,667
10
,867
98,933
14
151,067
4
37,767
12,667
10
1,267
Total
163,733
14
Between Groups
245,733
4
61,433
12,000
10
1,200
257,733
14
Within Groups
Total
Mingguketiga
Between Groups
Within Groups
Minggukeempat
Mean Square
Between Groups
Within Groups
Minggukedua
df
Within Groups
Total
F
Sig.
1,923
,183
19,750
,000
26,038
,000
29,816
,000
51,194
,000
60
Universitas Sumatera Utara
Lampiran 13 (Lanjutan)
Minggua_awal
a
Tukey HSD
kelompok
Subset for alpha = 0.05
N
1
Formula 1
3
38,3333
Formula 3
3
39,0000
Formula 2
3
39,3333
Formula 4
3
39,6667
Formula 0
3
40,3333
Sig.
,137
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_I
Tukey HSDa
kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
34,6667
Formula 3
3
36,3333
Formula 2
3
37,3333
Formula 1
3
38,0000
Formula 0
3
36,3333
40,3333
Sig.
,166
,166
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_II
a
Tukey HSD
kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
33,3333
Formula 3
3
34,3333
34,3333
Formula 2
3
35,0000
35,0000
Formula 1
3
Formula 0
3
Sig.
36,6667
40,3333
,257
,070
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
61
Universitas Sumatera Utara
Lampiran 13 (Lanjutan)
Minggu_III
a
Tukey HSD
kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
30,6667
Formula 3
3
32,3333
32,3333
Formula 2
3
33,3333
33,3333
Formula 1
3
Formula 0
3
34,3333
40,0000
Sig.
,091
,263
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_IV
a
Tukey HSD
kelompok
Subset for alpha = 0.05
N
1
2
Formula 4
3
28,6667
Formula 3
3
29,6667
Formula 2
3
31,0000
Formula 1
3
Formula 0
3
Sig.
3
31,0000
33,3333
40,0000
,142
,142
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
62
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Pori
One-Sample Kolmogorov-Smirnov Test
N
Minggu_
Minggu_
Minggu_
Minggu
Minggu_
awal
I
II
III
IV
15
15
15
15
15
Mean
25,8000
23,9333
22,6667
21,0000
19,6000
Std.
1,14642
2,12020
2,82000
3,60555
3,81351
Absolute
,236
,197
,215
,167
,142
Positive
,148
,154
,118
,124
,125
Negative
-,236
-,197
-,215
-,167
-,142
Kolmogorov-Smirnov Z
,914
,761
,833
,645
,549
Asymp. Sig. (2-tailed)
,374
,608
,491
,799
,924
a,b
Normal Parameters
Deviation
Most Extreme Differences
a. Test distribution is Normal.
ANOVA
Sum of Squares
Minggu_awal
Minggu_I
Minggu_II
Minggu_III
Between Groups
Mean Square
,400
4
,100
Within Groups
18,000
10
1,800
Total
18,400
14
Between Groups
44,933
4
11,233
Within Groups
18,000
10
1,800
Total
62,933
14
Between Groups
92,000
4
23,000
Within Groups
19,333
10
1,933
Total
111,333
14
Between Groups
154,000
4
38,500
28,000
10
2,800
Total
182,000
14
Between Groups
184,267
4
46,067
19,333
10
1,933
203,600
14
Within Groups
Minggu_IV
df
Within Groups
Total
F
Sig.
,056
,993
6,241
,009
11,897
,001
13,750
,000
23,828
,000
63
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Minggu_awal
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Formula 0
3
25,6667
Formula 3
3
25,6667
Formula 4
3
25,6667
Formula 1
3
26,0000
Formula 2
3
26,0000
Sig.
,998
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_I
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 4
3
21,0000
Formula 3
3
23,3333
23,3333
Formula 2
3
24,0000
24,0000
Formula 0
3
25,6667
Formula 1
3
25,6667
Sig.
,117
,280
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_II
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 4
3
Formula 3
3
22,3333
Formula 2
3
22,6667
Formula 1
3
24,3333
Formula 0
3
25,6667
Sig.
18,3333
1,000
,087
Means for groups in homogeneous subsets are displayed.
a.
Uses Harmonic Mean Sample
64
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Minggu_III
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
15,6667
Formula 3
3
20,6667
Formula 2
3
21,0000
Formula 1
3
22,0000
Formula 0
3
22,0000
25,6667
Sig.
1,000
,860
,127
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_IV
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
14,3333
Formula 3
3
18,0000
Formula 2
3
19,6667
Formula 1
3
21,0000
Formula 0
3
Sig.
18,0000
25,0000
,055
,135
1,000
Means for groups in homogeneous subsets are displayed.
65
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Spot
One-Sample Kolmogorov-Smirnov Test
N
Normal Parametersa,b
Most Extreme Differences
Mean
Std.
Deviation
Absolute
Positive
Negative
Mingguaw
al
Minggu I
15
15
42,8000 40,1333
1,32017 2,55976
,173
,173
-,160
,671
,760
Kolmogorov-Smirnov Z
Asymp. Sig. (2-tailed)
,187
,187
-,136
,726
,668
Minggu
II
15
36,1333
5,13902
Minggu
III
15
32,4000
7,89032
Minggu
IV
15
29,7333
9,27721
,158
,158
-,156
,613
,847
,120
,120
-,096
,466
,982
,132
,132
-,107
,509
,958
a. Test distribution is Normal.
ANOVA
Sum of Squares
Mingguawal
Minggu I
Minggu II
Between Groups
4
1,767
Within Groups
17,333
10
1,733
Total
24,400
14
Between Groups
71,067
4
17,767
Within Groups
20,667
10
2,067
Total
91,733
14
343,733
4
85,933
26,000
10
2,600
Total
369,733
14
Between Groups
842,933
4
210,733
28,667
10
2,867
871,600
14
1178,933
4
Between Groups
Within Groups
Total
Minggu IV
Mean Square
7,067
Within Groups
Minggu III
df
Between Groups
294,733
F
Sig.
1,019
,443
8,597
,003
33,051
,000
73,512
,000
113,35
,000
9
Within Groups
Total
26,000
10
1204,933
14
2,600
66
Universitas Sumatera Utara
Lampira 13(Lanjutan)
Minggu_awal
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Formula 2
3
42,0000
Formula 4
3
42,3333
Formula 3
3
42,6667
Formula 1
3
43,0000
Formula 0
3
44,0000
Sig.
,394
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_I
Tukey HSDa
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 4
3
37,3333
Formula 2
3
39,3333
Formula 3
3
39,6667
Formula 1
3
40,3333
Formula 0
3
40,3333
44,0000
Sig.
,153
,065
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_II
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
Formula 3
3
34,0000
Formula 2
3
36,3333
Formula 1
3
37,6667
Formula 0
3
Sig.
29,0000
43,6667
1,000
,109
1,000
Means for groups in homogeneous subsets are displayed.
67
Universitas Sumatera Utara
Minggu_II
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
29,0000
Formula 3
3
34,0000
Formula 2
3
36,3333
Formula 1
3
37,6667
Formula 0
3
43,6667
Sig.
1,000
,109
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_III
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
Formula 3
3
29,6667
Formula 2
3
31,3333
Formula 1
3
Formula 0
3
4
21,0000
36,3333
43,6667
Sig.
1,000
,749
1,000
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_IV
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
Formula 3
3
24,3333
Formula 2
3
27,6667
Formula 1
3
Formula 0
3
Sig.
4
18,0000
35,0000
43,6667
1,000
,159
1,000
1,000
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
68
Universitas Sumatera Utara
Lampiran 13(Lanjutan)
Keriput
One-Sample Kolmogorov-Smirnov Test
Minggua
wal
N
MingguI
MingguI
Minggu
I
II
IV
Minggu I
15
15
15
15
15
24,2667
22,6667
21,7333
21,2667
20,5333
,88372
1,91485
2,65832
2,98727
3,44065
Absolute
,219
,118
,142
,131
,114
Positive
,219
,118
,142
,109
,114
Negative
-,197
-,102
-,136
-,131
-,110
Kolmogorov-Smirnov Z
,847
,458
,550
,508
,443
Asymp. Sig. (2-tailed)
,471
,985
,923
,959
,989
a,b
Normal Parameters
Mean
Std. Deviation
Most Extreme Differences
a. Test distribution is Normal.
ANOVA
Sum of Squares
Minggu awal
Minggu I
Minggu II
Minggu III
Mean Square
F
Sig.
,654
,637
10,333 10,333
,001
Between Groups
2,267
4
,567
Within Groups
8,667
10
,867
Total
10,933
14
Between Groups
41,333
4
Within Groups
10,000
10
Total
51,333
14
Between Groups
80,933
4
Within Groups
18,000
10
Total
98,933
14
108,933
4
16,000
10
Total
124,933
14
Between Groups
149,733
4
16,000
10
165,733
14
Between Groups
Within Groups
Minggu IV
df
Within Groups
Total
1,000
20,233 11,241
,001
1,800
27,233 17,021
,000
1,600
37,433 23,396
,000
1,600
69
Universitas Sumatera Utara
Lampiran 13 (Lanjutan)
Minggu_awal
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
Formula 1
3
24,0000
Formula 3
3
24,0000
Formula 4
3
24,0000
Formula 2
3
24,3333
Formula 0
3
25,0000
Sig.
,689
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_I
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
20,3333
Formula 3
3
21,3333
21,3333
Formula 2
3
23,0000
23,0000
23,0000
Formula 1
3
23,6667
23,6667
Formula 0
3
25,0000
Sig.
,052
,098
,179
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_II
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 4
3
18,6667
Formula 3
3
19,6667
Formula 2
3
22,0000
Formula 1
3
23,3333
Formula 0
3
25,0000
Sig.
,073
22,0000
,117
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
70
Universitas Sumatera Utara
Lampiran 13 (Lanjutan)
Minggu_III
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
3
Formula 4
3
17,3333
Formula 3
3
19,3333
Formula 2
3
Formula 1
3
23,0000
Formula 0
3
25,0000
19,3333
21,6667
Sig.
,359
21,6667
,235
,055
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
Minggu_IV
a
Tukey HSD
Kelompok
Subset for alpha = 0.05
N
1
2
Formula 4
3
16,0000
Formula 3
3
18,3333
Formula 2
3
Formula 1
3
Formula 0
3
Sig.
3
4
18,3333
20,6667
20,6667
22,6667
22,6667
25,0000
,235
,235
,359
,235
Means for groups in homogeneous subsets are displayed.
a. Uses Harmonic Mean Sample Size = 3,000.
71
Universitas Sumatera Utara