Skrining Fitokimia dan Uji Aktivitas Antibakteri Ekstrak Daun Benalu Duku (Loranthus Sp)

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SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIBAKTERI
EKSTRAK DAUN BENALU DUKU (Loranthus Sp)

ABSTRAK

Telah dilakukan penelitian mengenai skrining fitokimia, aktivitas antibakteri dari
ekstrak etil asetat dari daun benalu duku (Loranthus Sp). Serbuk daun benalu duku
diekstraksi maserasi menggunakan pelarut metanol sehingga dihasilkan ekstrak
metanol sebanyak 50 g, ekstrak metanol dilarutkan dengan etil asetat dan
dihasilkan ekstrak etil asetat sebanyak 20 g. Berdasarkan skrining fitokimia
ekstrak metanol mengandung senyawa alkaloid, flavonoid, terpenoid dan tanin
sedangkan ekstrak etil asetat mengandung senyawa flavonoid. Terhadap ekstrak
etil asetat yang diperoleh dilakukan uji aktivitas antibakteri dengan metode difusi
cakram dengan konsentrasi 10%, 20%, 30%, 40%, dan sebagai pembanding
digunakan DMSO. Hasil uji ekstrak etil asetat menunjukkan aktivitas daya hambat
yang berbeda terhadap tumbuhan bakteri Escherichia coli dan Staphylococcus
aureus. Ekstrak etil asetat dengan konsentrasi 20%, 30%, 40% mampu
menghambat pertumbuhan bakteri Staphylococcus aureus 9,3 mm, 10,3 mm, 10,7
mm tetapi pada bakteri Escherichia coli tidak menunjukkan adanya zona hambat

pada bakteri.
Kata kunci : Daun benalu duku, Ekstrak Metanol, Ekstrak Etil Asetat, Antibakteri

Universitas Sumatera Utara

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PHYTOCHEMICAL SCREENING AND THE TESTED
ANTIBACTERIAL ACTIVITIES FROM EXTRACT LANSEH PARASITIC
LEAF (Loranthus Sp)

ABSTRACT

It had done the experiment of phytochemical screening, the antibacterial activity of
ethyl acetate extracts by lanseh parasite leaf (Loranthus Sp). The powder of lanseh
parasite leaf is extracted maceration using methanol to produce as much as 50 g of
methanol extract, methanol extract is diluted with ethyl acetate and ethyl acetate
extracts produced as much as 20 g. Based on phytochemical screening of the
methanol extract contains coumponds by alkaloids, flavonoids, terpenoids and
tannins, while on the ethyl acetate extract containing flavonoids. Against the ethyl

acetate extract obtained antibacterial activity test performed by disc diffusion
method with a concentration of 10%, 20%, 30%, 40%, and as a comparison is used
DMSO. The test results ethyl acetate extracts showing activity in distinct
inhibition against the growth of bacteria Escherichia coli and Staphylococcus
aureus. The ethyl acetate extract with a concentration of 20%, 30%, 40% were able
to inhibit the growth of Staphylococcus aureus 9.3 mm, 10.3 mm, 10.7 mm but the
bacteria Escherichia coli not show any inhibition zone in bacteria.
Keywords: Lanseh Parasite Leaf , Extract Methanol, Ethyl Acetate Extract,
Antibacterial

Universitas Sumatera Utara