Biol. Chem. Research. 43 Isolation of Saccharomyces Spp from Manure of Beef Bali Cattle as a Probiotics Properties and has CMC-ase Activity to Improve Nurient Quality of Rice Bran.
I solation of……………………………….Rice Bran Candraw ati, et al., 20 14 Calculations
The data w ere used t o calculat e AM E value according to t he follow ing form ulat e M ust afa et al., 2004: AM E apparent m et abolizable energy = IE – FE. Where IE = ingest ed energy;
FE = fecal energy voided by the fed birds.
St atistical Analysis
All dat a w ere subject ed t o a one-w ay analysis of variance t est St eel and Torrie, 1989. St at ist ical significances among t reat m ent means w ere det erm ined by m et hod of New
M ult iple Range Test of Duncan when t he F value w as significant at 5 level
RESULTS
Resilience isolat e Saccharomyces spp. t he low pH is one of t he charact erist ics required t o be met by a candidat e or a probiot ic that could be developed into a pot ential probiotic.
On t his t est , t he m edium pH w as adjust ed t o 1.5, 3.0, 4.5, and 6.0 by using HCl hydrochloric acid, because HCl has charact erist ics similar t o stom ach acid. Resist ant to
highly acidic nature needs t o be ow ned by t he candidat e probiotic, because t he applicat ion lat er, t his probiot ic candidat e must pass very acidic st omach conditions,
before reaching t he colon. Observat ions as present ed in Table 1. In this st udy, as m any as 10 isolat es Saccharomyces
spp . isolat ed from catt le feces w as t est ed resist ance t o various pH, at pH 1.5, 3.0, 4.5, and
6. Two isolat es w ere unable t o grow at pH 1.5, w hich isolates t he S-1 and S-2. Saccharomyces spp
.S-1 isolat es even sim ply could not grow at all pH t reat m ents given. Only 8 isolat es w ere able t o grow w ell, nam ely Saccharomyces spp.S-4 t o S-10 isolat es.
There is a t endency of t he higher pH, especially at pH 6, most of t he isolat es has decreased t he number of colony life. Saccharomyces spp colonies grow w ell at pH 3, even
isolat e S-3 and S-5 Saccharomyces spp colony number reached 5.531 and 5.093 log colony per gram .
Table 1. The number of colony isolates Sa ccharomyces spp Log colony g at various pH.
Code Isolat e pH
1.5 3
4.5 6
S
1
- -
- -
S
2
- 3.98
3.88 3.32
S
3
3.45 5.53
4.41 4.13
S
4
4.33 3.65
3.58 4.56
S
5
4.12 5.09
4.99 4.12
S
6
4.19 4.35
4.36 4.40
S
7
4.29 4.42
3.99 3.58
S
8
4.06 3.617
4.318 3.86
S
9
4.31 4.41
3.95 3.92
S
10
4.16 4.33
3.97 3.87
Descript ions: S-1 s d S-10 is a Saccharomyces spp isolat es w ere isolat ed from manure of beef Bali cat tle