Biol. Chem. Research. 41 Isolation of Saccharomyces Spp from Manure of Beef Bali Cattle as a Probiotics Properties and has CMC-ase Activity to Improve Nurient Quality of Rice Bran.
I solation of……………………………….Rice Bran Candraw ati, et al., 20 14
These NSPs are know n t o increase t he gut viscosit y, reduce nut rient absorpt ion in the intest ine and affect ed indirect ly t he growt h and perform ance of birds Rhames et al.
2006; Cao et al., 2003. M any st udies have clearly dem onst rat ed t hat , supplem ented of probiotics t o diet s rich in NSP result s in a significant reduction in t he int est inal viscosit y,
enhances energy, and prot ein ut ilization Bidura et al., 2012; Bidura et al., 2009. Probiot ics are defined as the viable microorganisms t hat exhibit a beneficial effect on
healt h of the host by improving it s int est inal microbial balance. Yeast culture is one of t he most ext ensively st udied probiotic. Tradit ionally, S.cerevesiae have been used for food
product s such as baking indust ries, “ t ape” , and are considered as organisms t hat can be used also for a pharmaceut ical Ahmad, 2005. The use of antibiot ics as routine feed
additives has been baned in sam e count ries because of public concern over possible antibiot ics residual effect s and the developm ent of drug-resistent bact eria. Probiotics
have been int roduced as an alt ernative t o ant ibiot ics; how ever, t heir effect s on poult ry product ion are not consist ent , result ing in uncert aint ies and skept icism for developm ent
of the product s. There are many t ypes of probiot ic preparat ions in the market . M any st udies have been conducted to t est t he afficacy of such preparat ions on anim al growt h
and performance. Several st udies w ith broiler have indicat ed that probiotics preparat ions im prove live w eight gain and feed conversion rat e Bidura et al., 2012; 2009; 2008;
Sut arpa et al., 2011. Hong et al. 2004 report ed t hat ferment at ion of feed using Aspergilus oryzae
increased digest ibilit y of it s DM and CP. Therefore, it is susgest ed t hat ferm ent ed of rice bran feeding by Saccharomyces spp
isolat es as a CM C-ase akct ivit y isolat ion from manure of beef Bali cat tle can be used in order t o alleviat e t he negative effect of rice bran as feed.
M ETERIAL AND M ETHODS
Animals and experimental design
Eight een of Lohman Brown laying hens w as assigned t o three t reat m ent s in a complet ely randomized design. Each t reat ment has six replications w ith one bird per replicat ion
individual cage. All of t he birds were fed experim ent al diet s for t w o days. The t reat m ent s w ere i unferm ent ed rice bran as control; ii ferment ed rice bran by
Saccharomyces spp.S-6 isolat e ; iii ferm ent ed rice bran by Saccharomyces spp.S-7 isolat e;
respect ively. The object ives of t his st udy are t o det ermine t he nut rient digest ibilit y and t he M E value of rice bran using Lohm an Brownn laying hens at 42 w eeks of age.
Probiotics properties Acid tolerance
: to det erm ine t he t rancsit t olerance t o low pH, the m et hod of Corzo and Gilliland 1999 was used with slight m odificat ions. St rain was grow n in M RS brot h at 37
C for 24 h. A 0.5 ml aliquot of t he bact erial culture w as inoculat ed in 10 m l of phosphat e
buffered saline adjust ed t o pH 2 with 4 N HCl. Phosphat e buffer w as prepared by dissolving NaCl 9 g l, Na
2
HPO
4
2H
2
O 9 g l, and KH
2
PO
4
1.5 g l in dest iled w at er. The initial bact erial concent ration w as 10
6-8
cfu m l. Cult ure w as incubat ed at 37 C. Aft er 0;
0.5; 1; 2; and 4 h incubation, cell w ere serially dilut ed t enfold in 0.1 M sodium phosphat e buffer pH 7.2, and t he viable cells w ere enumerat ed on M RS agar plat e at 37
C for 2 days.