I solation of……………………………….Rice Bran Candraw ati, et al., 20 14
In the t est of resist ance t o bile salt s as seen in Table 2 show s, that the t en isolat es w ere able to survive and grow on a medium cont aining bile salt s at concent rat ions of 0.2 m M
and 0.4 m M , whereas at a concent rat ion of 0.6 m M no isolat es can survive. M icrobial isolat es resist ance t o bile salt s is used t o assess t he abilit y t o survive t he digest ive t ract
isolat es cont ained bile salt s on the surface of t he int est ine. Probiot ics w ill be dealing w ith the environment in t he sm all int est ine, in which t here are
bile or bile salt s are rem oved by t he liver t hrough t he gall bladder, aft er successfully passing the acidic condit ions in the st om ach. Therefore, in t he process of developing new
probiotic, or a new probiotic candidat e m ust be able t o pass t he t est of resist ance t o bile or bile salt s w ere perform ed in vit ro. Based on t he nat ure of t he resist ance show n by
som e isolat es, st rai-indicat es t hat t he st rain has t he pot ential to be developed int o a pot ent ial probiot ic.
Table 2. Ability of yeast Sa ccharomyces spp isolates w ere resistant living in bile salts.
Isolat e Bile salt concent rat ion absorbance
0.2 mM 0.4 mM
0.6 mM S
1
+ 0.244 + 0.169
- 0.067 S
2
+ 0.248 + 0.168
- 0.059 S
3
+ 0.253 + 0.163
- 0.081 S
4
+ 0.252 + 0.158
- 0.064 S
5
+ 0.243 + 0.166
- 0.073 S
6
+ 0.224 + 0.144
- 0.036 S
7
+ 0.192 + 0.133
- 0.031 S
8
+ 0.145 + 0.151
- 0.051 S
9
+ 0.126 + 0.132
- 0.044 S
10
+ 0.138 + 0.149
- 0.035 Descript ion:
- :
A 0.1
not bear
bile salt
+ :
A 0.1
- 0.5
a bile
salt -resist ant +
+ :
A 0.5
- 1.0
bile salt -resist ant
+ +
+ :
A 1.0
highly resist ant
of bile
salt s S-1 s d S-10 is a Saccharomyces spp isolat es were isolat ed from m anure of beef
Bali cat tle CM C-ase t est is t o t est t he abilit y of t he yeast Saccharomyces spp isolat es in degrading
crude fiber. It can be m easured from t he result ing clear zone diam et er Table 3. From t he result s of t his st udy w ere only yeast Saccharomyces spp isolat es S-5, S-6, S-7, S-8, and the
yeast Saccharomyces spp.S-9 who has the abilit y t o digest fiber. Looks yeast Saccharomyces spp
isolat es S-6 has the most wide clear zone, while the S-8 has a clear zone at least .
J. Biol. Chem. Research. 44
Vol. 3 1, No. 1: 3 9-52 20 14
I solation of……………………………….Rice Bran Candraw ati, et al., 2 014
This m eans t hat isolat es S-6 has kem am puam in digest ing crude fiber t han the highest S- 8. M ore det ailed, clear zone diamet er and difference generat ed by t he t w o isolat es are
presented in Figure 1. Table 4 show s the nut rient the cont ent of crude prot ein and gross energy w ere slight ly
increased by ferm ent at ion. On the ot her hand ferm ent at ion caused decreasing dry mat t er DM , organic M at t er OM , and crude fibre CF of the rice bran. These result s
indicat ed t hat carbohydrat e w as used for m icrobial growt h and the reduct ion of dry mat t er. The result indicat ed that all of nut rient digest ibilit y of ferm ent ed rice bran by
Saccharomyces spp culture w ere increased significantly P0,05 different rat her t han
unferm ent ed rice bran.
Figure 1. Saccha romyces spp.S-8 isolates zone is smaller than the isolate Saccharomyces spp.S-6
right. Table 3. CM C-ase activity test yeast Saccha romyces spp isolates based on the diameter
of the clear zone caused.
Isolat Clear zone diam et er cm
S-
1
- S-
2
- S-
3
- S-
4
- S-
5
3.60 S-
6
4.51 S-
7
4.00 S-
8
1.85 S-
9
3.80 S-
10
- Descript ion: S-1 s d S-10 is a Saccharomyces spp isolat es w ere isolat ed from m anure of
beef Bali cat tle.
J. Biol. Chem. Research. 45
Vol. 3 1, No. 1: 39-5 2 2014
Isolate S
8
Isolate S
6
Clear zone
I solation of……………………………….Rice Bran Candraw ati, et al., 20 14
Table 4 show s t he nut rient digest ibility and m etabolizable energy of unferm ent ed rice bran UFR and ferm ent ed rice bran FR ingredient . The digest ibilit y of crude prot ein,
organic m at t er, and crude fiber, were slight ly increased significant ly different P0,05 by t he ferm ent at ion, bot h by Saccharomyces spp.S-6 and S-7, respectively. The
met abolizable energy of FR w as increased significantly different P005 than met abolizable energy of UFR. Ferm ent ed of feeding ingredient w ere caused increased of
crude prot ein, organic m at t er, and crude fibre digest ibilities, resvect ively t han UFR ingredient s.
Chem ical com posit ion and nut rient digest ibilit y of rice bran ferm ent ed compared t o t he unferm ent ed w ere show n in Table 4 as below:
Table 4. Chemical composition and nutrient digestibility of unfermented and fermented rice bran by Saccha romyces Spp.S-6 and S-7 isolates culture in Dry M atter.
Rice Bran Paramet ers
Unfer- ment ed
UFR Ferm ent ed
by S-6 Isolat e FR
Ferm ent e d by S-7
Isolat e SEM
Chemical composit ion: Dry M at t er
89.75a 89.64a
89.62a 0.453
Organic M att er 90.93a
90.87a 90.22a
0.318 Crude Prot ein
11.88b 14.69a
14.78a 0.149
Crude Fibre 15.96b
17.65a 18.19a
0.285 Gross Energy Kcal kg
3258.31a 3272.22a
3274.97a 44.528
Nut rient digest ibilit y : Dry mat t er digest ibilit y
25.48c 28.24b
29.81a 0.185
Organic M at t er digest ibilit y 26.16b
30.07a 30.25a
0.314 Crude Prot ein digest ibilit y
37.38b 51.77a
53.23a 1.106
Crude fibre digest ibilit y 15.92c
24.10b 26.29a
0.252 M et abolizable energy Kcal kg
1696.38b 1898.44a
1940.69a 19.512
Not e:
UFR = Unferm ent ed rice bran; FR = Ferm ent ed rice brang by 0.20 yeast cult ure
The different superscript at the sam e row is significantly different P0.05
DISCUSSION
The result s show ed t hat only 5 isolat es of Saccharomyces spp pot ent ial as probiotic agent s and have t he CM C-ase act ivit y. To five isolat es show ed resist ance t o acid and bile
salt s t hat is charact erist ic of probiot ic bacteria. Saccharomyces is a unicellular microorganisms lacking chlorophyll, grew well at 30
o
C and pH 4.8. pH value in t he gast roint est inal t ract of poult ry in each sect ion, are: crop 4.5, provent riculus 4.4,
J. Biol. Chem. Research. 46
